Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AE015451_phaM15,097,2400AC32.1% 268.3 / 16.0 196intergenic (+231/+61)PP_mr66 ncRNA (P6)/acsA‑I CDS (acetyl‑CoA synthetase)–/–
Reads supporting (aligned to +/- strand):  ref base A (115/16);  new base C (62/0);  total (180/16)
Fisher's exact test for biased strand distribution p-value = 1.79e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 200 total aligned reads displayed.

CACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCTTAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCGAG  >  AE015451_phaM1/5097105‑5097376
                                                                                                                                       |                                                                                                                                        
 aCCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCACGAATGCGTGAGGTCAGGGGACAGCTTAGc                                                                                                                                        >  1:16726591/1‑137 (MQ=255)
    gAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGATTGCCCGCGAATGCGTGAGGTCAGGGGACAGCTTaacatg                                                                                                                                     >  2:15704650/1‑132 (MQ=255)
    gAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGATTGCCCACGAATGCGTGAGGTCAGGGGACAGCTTCGCCTg                                                                                                                                     >  1:8221941/1‑137 (MQ=255)
      aaCGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGANAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGATTTCCCTCGAATGTGTGAGGTCAGGGGACAGTTTAGCCTGaa                                                                                                                                   >  1:16424219/1‑137 (MQ=255)
      aaCGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGGTTTCCCGCGAATGTGTGAGGTCAGGGGAAAGCTTCACCTgga                                                                                                                                   >  1:7543894/1‑135 (MQ=255)
      aaCGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGGTTGCCCACGAATGCGTGAGGTCAAGGGACAGCTTCGCCTTaa                                                                                                                                   >  1:10510626/1‑137 (MQ=255)
      aaCGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGGGGTTAGGGGGCAGCTTAGCCTGaa                                                                                                                                   >  2:6420607/1‑137 (MQ=255)
      aaCGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCTTAGCCTGaa                                                                                                                                   <  2:18086997/137‑1 (MQ=255)
      aaCGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGATTTCCCACGAATGCGTGGGGTCAGGGGGCAGCTTCGCCCGaa                                                                                                                                   >  1:7296813/1‑137 (MQ=255)
         gggCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCTTAGCCTGAACTg                                                                                                                                <  1:19215309/137‑1 (MQ=255)
         gggCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCTTAGCCTGAACTg                                                                                                                                <  1:4211433/137‑1 (MQ=255)
         gggCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCTTAGCCTGAACTg                                                                                                                                <  2:6991534/137‑1 (MQ=255)
         gggCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCTTAGCCTGAACTg                                                                                                                                <  2:2304692/137‑1 (MQ=255)
             cGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGATTGTCCGCGAACGAGTGAGGTTAGGGGACAACTTCGCCTGAACTCACGc                                                                                                                            >  1:14944551/1‑137 (MQ=255)
             cGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGATTGCCCGCGAATGCGTGAGGTCAGGGGACAGCTTCGCCTGAACTGACGc                                                                                                                            >  1:17555347/1‑137 (MQ=255)
             cGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCAGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCAAATGCGTGAGGTCAAGGGGCAGCTTGGCCTTAAATGACGc                                                                                                                            >  2:3161408/1‑137 (MQ=255)
              ggAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCTTAGCCTGAACTGACGCa                                                                                                                           <  2:1519437/137‑1 (MQ=255)
              ggAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTGGCCCTCGAATGCGTGAGGTCAGGGGACAGCTTAGCCTGAACTGACGCa                                                                                                                           <  1:3841974/137‑1 (MQ=255)
              ggAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGATTGTCCACGAATGCGTGGGGTCAGGGGACAGCTTCGTCTGAACTGACCCa                                                                                                                           >  1:4388945/1‑137 (MQ=255)
               gggttGTTTTCTGGCTCCCCAAACCTGTTCTGGGCCGGGGACAGAACAGTACCCGATGATTGTCGGGTAACAGTGCAAAAGTGGGCGTGCCCGCGAATGCGTGAGGTTGGGGGACGGCTGAGCCTGAACGGACGCAt                                                                                                                          <  2:13928027/134‑1 (MQ=255)
                        ccTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCACGAATGCGTGAGGTCAGGGGAAAGCTTAGCATGAAATGACACACTCACgggaa                                                                                                                 >  1:4398049/1‑135 (MQ=255)
                             cgcCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGGTTGGCCACGAACGCGTGAGGTCAGGGGAACGCCTAGCCTGAACCGACACATTCGCTGGCAAGccc                                                                                                            >  1:12376466/1‑137 (MQ=255)
                               ccccGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGATTAGCCTGAACTTACACCTTCTCGGGCAAGCCCGc                                                                                                          >  2:10300313/1‑137 (MQ=255)
                                  cGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTTCCCGCGAACGCGTGAGGTCAGGGGACAGACTCGCCCGAACTGACGCCTTCGCAGGAAAACCCACTcc                                                                                                       >  1:6771444/1‑137 (MQ=255)
                                  cGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCACGAATGCGTGAGGTCAGGGGAAAGCTTAGCCTGAACTGACGCATTTGCGGGCAAGCCCGCTcc                                                                                                       >  2:1101088/1‑137 (MQ=255)
                                     aCCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGGGTGAGGTCAGGGGACAGATTCACCCGAACTGACCCATTCGTGGGCAAGACCGTTCCTGc                                                                                                    >  1:17044497/1‑137 (MQ=255)
                                       cTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAGCAATGCAAAAGCGGGGTTGCCCGCGAATGTGTGGGGTCAGGGGAAAGATTCGCCTGAACTGACGCAGTCGCAGGCAAGCCCGCTCCTGccg                                                                                                  >  2:5191537/1‑135 (MQ=255)
                                             ggggCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTTCCCGCGAATGCGTGAGGTCAGGGGACAGCTTAGCCTGAACCGACGCATTCGCGGGCAAGCCCGCTCCTTCAGGGGGTa                                                                                            >  2:6804761/1‑137 (MQ=255)
                                             ggggCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTTCCCGCGAATGCGTGAGGTCAGGGGAAAGCTTCGCCTGAACTTACGCCCTCCCTGGCAAACCCCCTCCTGCCggggggc                                                                                            >  2:5250695/1‑135 (MQ=255)
                                             ggggCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTTCCCGCGAATGAGTGAGGTCAGGGGACAGCTTCGCCTGAACCGACGCACTCTCGGGTCAACCCACTCCTGCAGGGGGtg                                                                                            >  1:9169072/1‑136 (MQ=255)
                                             ggggCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTTCCCACGAATGCGTGAGGTCAGGGGAAAGCTTCGCCCGGACTGACACATTCGCGGGCAAACCCGCTCCTGCAGGGGGTa                                                                                            >  2:19120709/1‑137 (MQ=255)
                                             ggggCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGGGAATGCGTGAGGTCAGGGGACAGCTTAGCCTGAACTGACTCATTCGCGGGCAAACCCGCTCCTGCAGGGGGTa                                                                                            >  2:18501409/1‑137 (MQ=255)
                                             ggggCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGAAAGCTTCGCCTGAACTGACGCATTCTCGGGCAAGCCCGCTCCTGGAgggggga                                                                                            >  2:2257509/1‑135 (MQ=39)
                                             ggggCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGAAAGCTTAGACTGAACTGACGCATTCGCGGGAAAGCCCACTCCTGCAGGGGGTa                                                                                            >  1:12184992/1‑137 (MQ=255)
                                             ggggCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAAGGGACAGATTCGCCCGAACCGACGTATTCTCGGGCAAACCCGCTCCTGCCgggggga                                                                                            >  2:8583149/1‑135 (MQ=255)
                                             ggggCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAACGCGTGAGGTCCGGGGACAGCTTAAACTGAACCGACGCACTCGAGGGCAAGCCCGCTCCTGCAgggggga                                                                                            >  1:3590108/1‑135 (MQ=255)
                                             ggggCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCCTGCCCCCGAATACCCTAGGTCAAGGGACAGCTTCCCCCAAACCGACGCCCTCACTGGCAAACCCCCCCCCGCAgggggga                                                                                            >  1:5136910/1‑135 (MQ=255)
                                             ggggCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGATTTCCCGCGAATGCGTGAGGTCAGGGGAAAAATTCGCCTGAACCGACGCATTCTCGGGCAAACCCCCTCCTGCAAGGGGTa                                                                                            >  1:4186681/1‑137 (MQ=255)
                                             ggggCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGATTGCCCGCGAATGAGTGAGGTCAGGGGAAAACTTAGCATGAAATGACGCATTCGCGAGCAAGCCAGCTCCTGCAgggggga                                                                                            >  2:10057240/1‑135 (MQ=255)
                                             ggggCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGATTGCCCGCGAACGCGTGAGGGCAGGGGAAAGATTCGCCCGAACCGACACACTCTCGGGCAAACCCACTCCTTCAAAGGGTa                                                                                            >  1:3190682/1‑137 (MQ=255)
                                             ggggCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGATTGCCCAGGACTGCGTGAGGTCAAGGGAAAGCCTCGCCCGCACCGACTCATTCGCAGGCCAGCCCGCTCCTGTAGGGGGTa                                                                                            >  1:17117251/1‑137 (MQ=255)
                                             ggggCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGATTGCCCACGAATGCGTGAGGTCAGGGGACAGATTAGCCCGAACCGACGCACTCTCGGGCAAACCCACTCCTGCAAGGGGtg                                                                                            >  1:17425030/1‑136 (MQ=255)
                                                 ccGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCACGAATGCGTGAGGTCAGGGGACAGCTTAGCCTGAACTGACACATTCGCGGGCAAGCCCGCTCCCGCAGGGGGTATTGc                                                                                        >  2:14050463/1‑137 (MQ=255)
                                                 ccGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCACGCAAATGCGTGAGGTCAGGGGACAGCTTCGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGc                                                                                        >  2:12776624/1‑137 (MQ=255)
                                                  cGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGGTTGCCCGCGAATGCGTGAGGTCCGGGGACAACTTAGCCTGAAATGACGCACTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGcc                                                                                       >  2:2772505/1‑137 (MQ=255)
                                                  cGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTTCCCGCGAATGAATGAGGTCAGGGGAAAGCTTCGACTGAACTTACACATTCGCGGGCAAGACCTCTCCTGCAAGGGGTATTTcc                                                                                       >  1:8901571/1‑137 (MQ=255)
                                                  cGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGGGGTCAGGGGACAGCTTCGCCCGAACCTACACCTTCTCAGGCAAACCCACTCCCGCAAGGGGTATTGcc                                                                                       >  1:12717518/1‑137 (MQ=255)
                                                  cGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTTAGGGGACAACTTCGCCTGAACTGGCGCACTCGCGGGCAAACCCTCTCCTACAGGGGGTATTGcc                                                                                       >  1:3636596/1‑137 (MQ=255)
                                                  cGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCTTAGCCTGAACTGACGCATTAGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGcc                                                                                       >  1:15460977/1‑137 (MQ=255)
                                                  cGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCACGAATGCGTGAGGTCAGGGGACAGCTTCGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTTcc                                                                                       >  2:12241512/1‑137 (MQ=255)
                                                  cGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCACGAATGCGTGAGGTCAGGGGACAGCTTAGCCTGAAACGACGCACTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGcc                                                                                       >  2:16063614/1‑137 (MQ=255)
                                                    gTGACAGAACAGTAACCGATGATTGTCGGGTAACAATTCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCTTAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCgg                                                                                     >  1:16172323/1‑137 (MQ=255)
                                                    gTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCCGGGGACAGCTTAGCCTGAACTGACGCACTCGCGGGCAAGCCCACTCCTGCCAGGGGTATTGCCgg                                                                                     >  2:12984569/1‑137 (MQ=255)
                                                       aCAGAACAGTAACCGATGATTGTCGGGTAACAATTCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCTTAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGaa                                                                                  >  2:15396367/1‑137 (MQ=255)
                                                       aCAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTTCCCGCGAACGCGTGAGGTCAGGGGACAGCTTCGCCTGAACTGACGCATTCGCGGGCAAGCCCACTCCTGCAAGGGGTATTGCCgggga                                                                                  >  1:1594062/1‑135 (MQ=39)
                                                       aCAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCTTAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAAGGGGTATTGCCAGGaa                                                                                  >  2:6253257/1‑137 (MQ=255)
                                                       aCAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAAGGGACAACCTCACCTTAACTTGCACATTCTTGGGCAAGCCCGTTCCTGCAGGGGGTATTGCCgggga                                                                                  >  2:13280823/1‑135 (MQ=255)
                                                       aCAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAACGCGTGAGGTCCGGGGACAGCTTCGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGAAGGGGGTATTTCCGGGaa                                                                                  >  2:9764483/1‑137 (MQ=39)
                                                       aCAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAACGCGTGAGGTCCAGGGACAGGTTAGCCTGAACTGACACACTTGCGGGCAAACCCACTCCTGCAGGGGGTATTGCCGGGaa                                                                                  >  1:6455876/1‑137 (MQ=255)
                                                       aCAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCATGAATGCGTGAGGTCAGGGGAAAGCTTAACCTGAACTGACGCACTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCAGGaa                                                                                  >  2:8686337/1‑137 (MQ=255)
                                                       aCAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCACGAATGCGTGAGGTCGGGGGACAGCTTCGCCCTAACTGACGCACTCTCGGGCCAGCCCTCTCCTGCAGGGGGTGTTGCCGGGaa                                                                                  >  1:136207/1‑137 (MQ=255)
                                                       aCAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGATTGCCCGCGAATGCGTGNGGTTAGGGGACAGCTTAGCCCGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCCGGGGGGATTGCCGGGaa                                                                                  >  1:18003461/1‑137 (MQ=255)
                                                       aCAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGATTGCCCGCGAATGCGTGAGGTCAGGGGAAAGCTTAGCCTGGACTGACGCATTCGCGGGTAAACCCGCTCCTTCAGGGGGTATTGCCGGGaa                                                                                  >  1:16680481/1‑137 (MQ=39)
                                                            aCAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGTTTTCCCGCGAATGCGTTAGGTTAGGGGACAGCTTCGCCTTAAACGACGCATTCGCTGGCAAGCCCGTTCCTTCAGGGGGGATTGCCCGGAAGGGtt                                                                             >  1:12855589/1‑137 (MQ=255)
                                                            aCAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTTCCCGCGAATGCGTGAGGTCAGGGGACAGCTTAGCCTGAACTGACGCATTCGCGGGCAAACCCACTCCTGTAGGGGGTATTGCCGGGAAGGGtt                                                                             >  2:11597501/1‑137 (MQ=255)
                                                              aGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCTTCGCCTGAACCGACGCATTCGCGGGCAAACCCGCTCCTTCAGGGGGTATTGCCCGGAAGGGtttc                                                                           >  1:10050805/1‑136 (MQ=39)
                                                              aGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAACTTCGCCCGAACTGACACACTCGCGGGCAAACCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGtttc                                                                           >  1:8286612/1‑136 (MQ=255)
                                                              aGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCAAACGCGTGAGGTCAGGGGACAGCTTAACCCGAACTGACGCACTCCCGGGCAAACCCACTCCTGCAAGGGGTATTTCCGGGAAGGGtttc                                                                           >  1:7585028/1‑136 (MQ=255)
                                                              aGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCACGAATGCGTGAGGTCCGGGGACAACTTCGCCTGAACCAACGCCTTCTCGGGCAAGCCCACTCCCGCAAGGGGTATTGCCGGGAAGGGtttc                                                                           >  1:1409316/1‑136 (MQ=255)
                                                              aGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGATTGCCCGCGAATGAGTGAGGTTAGGGGGCAGATTAACCCGAACCGACGCACTCCCCGGAAAACCCACTCCCTCCGGGGGTATTGCCAGGAAGGGtttc                                                                           >  1:5055269/1‑136 (MQ=255)
                                                                 aaCCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGAAAGATTCGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGc                                                                        >  2:17180434/1‑137 (MQ=255)
                                                                  aCCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTTCCCCCAAATGAATGAAGTCCGGGGACAACTTCGCCTGAACCGACGCCTTCTCGGGCAAGCCCGCTTCTTCAGGGGGTATTGCCAGGAAGGGTTTCCGcc                                                                       >  2:7155896/1‑137 (MQ=255)
                                                                    cGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCACGAACGCGTGAGGTCAGGGGACAGCTTAGCCCGAAATGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTCTTGCCGGGAAAGGTTTCCGCCGa                                                                     >  2:9329147/1‑137 (MQ=255)
                                                                          ttGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAACGCGTGAAGTCAGGGGACAACTTAACCTGAAATAAAGCCTTTGCGGGAAAACCCGCCCCCACAAGGGGTATTGCCTGGAAGGGGTTCCGCCGCCGCCaa                                                               >  2:12516386/1‑136 (MQ=255)
                                                                           tGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCTTAGCCTTAACTGACGTATTCTCGGGCAAGCCCACTCCTGCAGGGGGTATTGCCGGGAAGGGTTTCCTCCCAAGCCaag                                                              >  2:2537817/1‑135 (MQ=255)
                                                                                        tGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCTTAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCCGCAGGGGGTGTTGCCGGGAAGGGTTTACGCCGAAACCAGGTTCATCGCCTtgt                                                 >  2:10999894/1‑137 (MQ=255)
                                                                                        tGCAAAAGCGGGCTTGCCCACGAATGCGTGAGGTCAGGGGACAGCTTCGCCTGAACTGACGCATTTGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAACCAAGTTCACCGCATtgt                                                 >  2:14027377/1‑137 (MQ=255)
                                                                                          cattcgCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCTTAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTgg                                               >  2:3341620/6‑137 (MQ=255)
                                                                                                     tttcccGCGAATGCGTGAGGTCAGGGGACAGCTTCGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGTAGGGGGTATTGCCGGGAAGGGTTTCCGCCGAAGCCAGGTTCATCGACTTGTGGGTATCGATCAg                                    >  2:4390091/4‑137 (MQ=255)
                                                                                                          cgcgAATGCGTGAGGTCAGGGGACAGCTTAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCt                               >  1:5845683/1‑137 (MQ=255)
                                                                                                                     gAGGTCAGGGGACAGCTTAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACAccc                    >  1:6937932/1‑137 (MQ=255)
                                                                                                                     gAGGTCAGGGGACAGCTTAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACAccc                    >  1:6687307/1‑137 (MQ=255)
                                                                                                                              ggACAGCTTAGCCTGAACTGACGCATTCGCGGGCCAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTCTCGATCAGGTGCTGTACCACACCCGGGTCAGcc           >  1:9165859/1‑137 (MQ=255)
                                                                                                                              ggACAGCTTAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGcc           >  2:13160623/1‑137 (MQ=255)
                                                                                                                              ggACAGCTTAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGcc           >  1:16930088/1‑137 (MQ=255)
                                                                                                                              ggACAGCTTAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGcc           >  2:14658100/1‑137 (MQ=255)
                                                                                                                              ggACAGCTTAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGcc           >  2:15308103/1‑137 (MQ=255)
                                                                                                                               gACAGCTTAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCa          >  1:19017766/1‑137 (MQ=255)
                                                                                                                               gACAGCTTAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCa          >  1:14289616/1‑137 (MQ=255)
                                                                                                                                aCAGCTTAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCTGGTCAGCCaa         >  1:11735624/1‑137 (MQ=255)
                                                                                                                                     ttAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCg    >  2:5917169/1‑137 (MQ=255)
                                                                                                                                     ttAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCg    >  1:13221239/1‑137 (MQ=255)
                                                                                                                                     ttAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCg    >  2:3438220/1‑137 (MQ=255)
                                                                                                                                     ttAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCg    >  2:9433228/1‑137 (MQ=255)
                                                                                                                                     ttAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCg    >  1:19447899/1‑137 (MQ=255)
                                                                                                                                      tAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCga   >  1:12464162/1‑137 (MQ=255)
                                                                                                                                      tAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCga   >  1:15542376/1‑137 (MQ=255)
                                                                                                                                      tAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCga   >  2:9125765/1‑137 (MQ=255)
                                                                                                                                      tAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCga   >  1:4587448/1‑137 (MQ=255)
                                                                                                                                       aGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCgag  >  1:6208261/1‑137 (MQ=255)
                                                                                                                                       |                                                                                                                                        
CACCGAAACGGGCCGGAATTGTTTCCTGGCGCCCCGAACCTGTGCGGGGCCGGTGACAGAACAGTAACCGATGATTGTCGGGTAACAATGCAAAAGCGGGCTTGCCCGCGAATGCGTGAGGTCAGGGGACAGCTTAGCCTGAACTGACGCATTCGCGGGCAAGCCCGCTCCTGCAGGGGGTATTGCCGGGAAGGGTTTACGCCGAAGCCAGGTTCATCGCCTTGTGGGTATCGATCAGGTGCTGTACCACACCCGGGTCAGCCAAGGTCGAG  >  AE015451_phaM1/5097105‑5097376

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑