| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | AE015451_phaM1 | 1,499,498 | 1 bp→CG | intergenic (+75/‑89) | trpS CDS (Tryptophan‑‑tRNA ligase) → / → zapE CDS (putative enzyme with nucleoside triphosphate hydrolase domain) | –/– |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | AE015451_phaM1 | 1,499,497 | 1 | . | C | 77.7% | 573.3 / 277.5 | 313 | intergenic (+74/‑90) | trpS CDS (Tryptophan‑‑tRNA ligase)/zapE CDS (putative enzyme with nucleoside triphosphate hydrolase domain) | –/– |
| Reads supporting (aligned to +/- strand): ref base . (31/38); new base C (172/68); total (203/110) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.19e-05 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| * | AE015451_phaM1 | 1,499,498 | 0 | T | G | 84.1% | 532.9 / 36.3 | 313 | intergenic (+75/‑89) | trpS CDS (Tryptophan‑‑tRNA ligase)/zapE CDS (putative enzyme with nucleoside triphosphate hydrolase domain) | –/– |
| Reads supporting (aligned to +/- strand): ref base T (33/11); new base G (159/74); total (203/110) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.76e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
Only 100 of 324 total aligned reads displayed.
TGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCCAC‑GGCTTTGCCGGTG‑T‑T‑T‑CGGG‑CAC‑G‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACT > AE015451_phaM1/1499363‑1499632 ||| aatcccTTGATGCGGCCGTTAAGAGCCTGAACGAATCCTTGGAACCACAGCAGGACTGAGGCTGCAAACCGGTGGGAGCGGGCGTGCCTGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGCTTTGCCGGTG‑t‑t‑tgcg < 2:358662/134‑4 (MQ=21) cGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGCTTTGCCGGTG‑T‑TCG‑CGGG‑CAc > 2:6058457/1‑137 (MQ=37) gATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGGGTGTCCGCGAAACAAGAGCCGCGGTGCATGGCACC‑GGTTTTTCCGGTG‑T‑T‑TACGGG‑CAc‑g‑cc > 1:6044929/1‑137 (MQ=255) gATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGCTTTGTCGGTG‑T‑TCA‑CGGG‑CAc‑g‑cc > 2:9254722/1‑137 (MQ=25) gATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCACGAAACAGGCGCCGCGGTGCACGGCACC‑GGCCTTGCCAGTG‑T‑TCG‑CGGG‑CAc‑g‑cc > 2:16777977/1‑137 (MQ=25) gATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGAGTGGCCGCGAAAAAAGAGCCACAGTGCCCGGCACC‑GAATTTTCCAGTGTT‑T‑T‑CGGG‑CAc‑a‑cc > 1:7548722/1‑137 (MQ=255) tGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGTCGCGGTGCATGGCACC‑GGGCTTGCCGGTG‑T‑TCG‑CGGG‑CAc‑gtccg > 2:11577767/1‑133 (MQ=25) ctaTTCAGAGCTTGAACGAAGCCTGGGCACCACAGCAGGACTGAGGCTGCCAAACGGGGGGAGTGGGTGTGCCCGCGAAACGGGCGCTGCTGGGCCTGGCACT‑GGTTTTGCTGGTG‑T‑TAG‑CGGG‑CAC‑GCCCGCTccc < 2:12141575/135‑1 (MQ=255) aTTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCAGTGCACGGCACC‑GGCTTTGCCGGTG‑T‑TCG‑CGGG‑CAC‑GCCCGCTCCcac > 2:14394989/1‑137 (MQ=21) aTTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCAAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGCTTTGCCGGTG‑T‑TCG‑CGGG‑CAC‑GTCCGCTCCcac > 2:6933881/1‑137 (MQ=21) gAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCAAAAAAAGCGCCACGGTGCACGGCCCC‑TGATCTGCCGGTT‑T‑TCG‑CGGG‑CAC‑GCCCGCTCACCCAGAATGATTGc > 1:14574739/1‑137 (MQ=19) aCGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCATGCCCACGAAACAAGCGCCGCGGTGCATGGCACC‑GGCTTTGCCGGTG‑T‑TCG‑CGGG‑CAC‑GCCCGTTCCCACAAACTGAGTGCCa > 2:16935062/1‑137 (MQ=18) cGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGCTTTGCCGGTG‑T‑TCG‑CGGG‑CAC‑GCCCGCTCCCACAGACTGATTGCCAt > 2:4475608/1‑137 (MQ=21) cGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAAAAAGCGCCACGGTGCCTGGCACC‑GGCTTTGTCGGTG‑T‑TCT‑CGGG‑CCC‑GCCCGCTCCCACAGACTGATTGCCAt > 2:10207892/1‑137 (MQ=21) cGAAGCCTTGGCACCAAAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGCAACAAGCGCCTCGGTGCATGGCAAC‑GGTTTTGCCAGTA‑T‑TCG‑CGGG‑CACAA‑CCGCTCCCACAGACCGGTTTCCAt > 2:12339615/1‑137 (MQ=18) ggcaccTTGTCACCACAATAATACTGAGTTGACCAAATGTTGGAGGCGGGAGTGCCCGCGAAAAAGGCGCCGTGGTGCAGGGCCCT‑GGTTGTTTAGGTG‑T‑T‑C‑GGGGACAA‑GCCCGCTCCCACAGACTGATTGCCAtt < 2:5112977/133‑1 (MQ=255) gAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGTGTGTCCGCGAAAAAGGCGCCACGGTGCCTGGCACC‑AGCTTTTTCGGTG‑T‑TCT‑CAGG‑CACAC‑CCACCCCCACAAAATGATTGCCAtt > 1:14486795/1‑137 (MQ=255) gAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGGGTGTCCGCGAAACAAGAGCCGCAGTGCCTGGCACC‑GGCTTTTCCGGTGTT‑T‑T‑TGGG‑CAC‑GCCCGCTCCCCCAAAACGAGTGCCact > 1:13031249/1‑135 (MQ=255) gAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGGGTGTCCGCGAAAAAAGCGCCGTGGTGCCCGGCACC‑TGCTTTGCCGGTG‑T‑T‑TGCGGG‑CAC‑GCCCGCTCCCACCAACCGAGTGCCAtt > 1:17826374/1‑137 (MQ=19) gAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCTCGAAAAAGGAGCCGCGGTGCATGGCACC‑GGCTTTGCCGGTG‑T‑TCG‑CGGG‑CAC‑GCCCGCTCCCACAGACTGATTGCCAtt > 1:13518502/1‑137 (MQ=21) gAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGTATGGCACC‑GGCTTTGCCGGTG‑T‑TCG‑CGGG‑CAC‑GCCCGCTCCCACAGACTGATTGCCAtt > 2:17841217/1‑137 (MQ=21) gAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCACGAAACAGGCGCCGCGGTGCCTGGCACC‑GGCTTTGCCGGTG‑T‑TCG‑CGGG‑CAC‑GCCCGCTCCCACAGAATGATTGCCAtt > 2:17929630/1‑137 (MQ=21) ccTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAAAAGGCGCCGCGGTGCATGGCAAC‑GGATTTGCCAGTG‑T‑TCG‑CGGG‑CAC‑GCCCGCTCCCACAGACCGATTGCCATTCAGc > 2:17165786/1‑137 (MQ=21) ccTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCACGAAAAAAGCGCCACGGTGCACGGCACC‑GGATTTTTCGGTG‑T‑TCT‑CGGG‑CAC‑GCCCACTCCCACAAACTGATTGCCCTTAAga > 1:9116470/1‑136 (MQ=255) ttGGCACCACAGAAGGATTGGGGTGGCAAAATGGTGGGAGTGGGTGTTCCCGAGAAACAGGCGCTG‑GGGGGAGGGGCACGGGTTTTGCTGGTG‑T‑T‑TGGGGG‑CAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCgg < 1:1105792/137‑1 (MQ=255) gCACCACAGCAGGACTGAGGCTGCCAAACGGGGGGAGCGGGCGGGCCCGCGAAAAAGGCGCCGCGGTGCATGGCACT‑GGCTTTCCCGGTG‑T‑TCT‑CGGG‑CAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGcc < 2:2605680/137‑1 (MQ=25) gCACCAAAGCAGGACTGAGGTTGCCAAACGGTGGGAGCGGGCGTGCCTGCGAAAAGGGCGCCGCGGTGCATGGCACC‑GGCTTTGCCGGTG‑T‑T‑CGCGGG‑CAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGcc < 2:4859200/137‑1 (MQ=18) gCACCAAAGAAGGATTGAGGCGGCAAAATGGTGGGAGTGGGTGTTCCCGCGAAAAGGGCCCGGTGGTG‑AGGGGAACTGGTTTTGCTGGTG‑T‑T‑TGTGGG‑CAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGcc < 1:18697115/137‑1 (MQ=255) gCACAACAGCAGGATTGGGGTTGCCAAATGGTGGGACTGGGCGGGCCCGGGAAACGGGCTCTGGGGGGCGGGGCACT‑GGTTTTGCTGGTG‑T‑T‑TGTGGG‑CAC‑TCCCGCTCCCACAGACTGATTGCCATTAAGCGGGcc < 1:13896797/137‑1 (MQ=255) caccacAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGGCCGTGAAACAGGCGCCGCGGTGCATGGCACC‑GGCTTTGCCGGTG‑T‑TCG‑CAGG‑CAC‑ACCCGCCCCCACAGACTGATTGCCATTCAGCGGGCCt > 2:3166273/1‑137 (MQ=21) caccacAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGCTTTGTCGGTG‑T‑TCG‑CGGG‑CAC‑GCCCGCTCCCACAGACTGATTGCCCTTCAGCGGGCCt > 2:13300600/1‑137 (MQ=18) caccacAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGCTTTGCCGGTG‑T‑T‑CGCGGG‑CAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGACt > 2:19088371/1‑137 (MQ=21) caccacAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAAAAGGCGCCGCGGTGCCTGGCACC‑GGCTTTGCCGGTG‑T‑T‑CGCGGG‑CAC‑GCCCGATCCCACAGACTGATTGCCCTTAAGCGGGctt > 2:8626533/1‑135 (MQ=18) aaccacAGAAGGACGGAGGCTACAAAATGGGGGGAGCGGGTGTGCCTGCGAAACGGGCGCCGCGGTGCATGGAACC‑GGTTTTGCTGGTG‑T‑TCG‑CGGG‑CAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCt < 2:7794666/136‑1 (MQ=16) accacAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAAGCGCCACGGTGCACGGCACC‑AAATTTTCCAGTG‑T‑TCG‑CGGG‑CAC‑GCCCACCCCCCCAGACTGATTGCCATTCAGCGGGCCTa > 1:7062510/1‑137 (MQ=16) acaGCAGGACTAAGGCTGCCAAACGGTGGGAATGGGCGTTCCCGCAAAACATGCGCCGCGGTACATGACACC‑GGCTTTGCCGGTG‑T‑TCG‑CGGG‑CAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCg < 2:7616976/137‑1 (MQ=21) acaGCAGAACGGAGGTGGCAAAAGGGGGGGGGTGGGTGTGCCCGAAAAACAGGCACCGGGGGGCATGGCACC‑GGTTTTGCTGGTG‑T‑C‑CGAGGG‑CAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCg < 1:18358297/137‑1 (MQ=12) gcagGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGCTTTGCCGGTG‑T‑TCG‑CGGG‑CAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTa > 2:9120134/1‑137 (MQ=21) gcagGACTGAGGCTGCCAAACGGTGGGAGCGGGAGTGCCCGCGAAACAAGCGCCGCGGTGTACGGCACC‑AGCTTTTCCGGTG‑T‑TCG‑CGGG‑CACAC‑CCGCTCCCCCAAACCGACTTCCACTCAGCGGGCCTATCGCtc > 2:1613244/1‑136 (MQ=255) gACTGAGGCTGCCAAACGGTGGGAGCGGGAGTGCCCACGAAAAAAGCGCCGCGGTGCATGGCCCC‑GGCCTTTCCGGTG‑T‑TCG‑CGGG‑CAC‑ACCCACCCCCACAAACCGATTTCCCTTCAACAGGCCTCTCGCTAccat > 1:9433310/1‑134 (MQ=255) tGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAAGAGCCACGGTTTATTGCCCC‑CGCTTTTCCGGTG‑T‑TCG‑CTGG‑CAC‑GCCCGCTCCCACAGACCGATTGCCATTCAGCGGGCCTGTTGCTACCGTGAc > 2:14682684/1‑137 (MQ=12) ctacaaaaCGGGGGGGGGGGGGGTGCCCGAGAAACAGGCGCTGCGGGGAATGGCACT‑GTTTTTTCCGGTG‑T‑TCG‑TGGG‑CAC‑CCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGccc < 1:17479890/132‑1 (MQ=255) cTGCCAAACGGTGGGAGCGGGCGTGCCCTCGAAACAGGCGCCACGGTGTATGGCACC‑GGCTTTGCCGGTG‑T‑TCG‑CGGG‑CAC‑GCCCGCTCCCACAAACTGATTGCCATTCAGCGGGCCTCTCGCTCCAATGACGGccc > 2:14412365/1‑137 (MQ=17) cTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGCTTTGCCGGTG‑T‑TCG‑CGGG‑CAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGccc > 2:19273542/1‑137 (MQ=21) cTGCCAAACGGTGGGAGCGGGCGTGCCCACGAAACAAGCGCCGCGGTGCATGGCACC‑GGCTTTTCCGGTG‑T‑TCT‑CGGG‑CACAC‑CCGCTCCCACAGACTGATTGCCACTCAACGGGCCTCTCGCTCCAGTGACAGccc > 2:13153187/1‑137 (MQ=17) gCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGATTTGTCGGTG‑T‑TCG‑CGGG‑AACAC‑CCACTCCCAAAAACTGAGTGCCATTAAACGGGCCTCTCGCTACAGTGGCGGcccat > 2:13582731/1‑135 (MQ=12) gCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCACCGCGGTGCATGGCACC‑GGATTTGCCGGTG‑T‑T‑CGCGGG‑CAC‑GCCCGCTCCAAAAGAATGATTGCCATTAAGCGGGCCTATCGTTACAGTGGAGGCCCGt > 2:18591763/1‑137 (MQ=17) aaaCGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCAGTGCATGGCCCC‑GGCTTTGCCGGTG‑T‑TCG‑CGGG‑CAC‑GCCCGCTCCCACAGACTGACTGCCATTAAGAAGGACTATTGCTAAAGTGACGGTCCGtttt > 2:9729314/1‑137 (MQ=17) aaCGGTGGGAGCGGGCGTGTCCACGAAACAGGCGCCGCGGTGCATGGCACC‑GGCCTTGCCGGTG‑T‑TCG‑CAGG‑CACAC‑CCACTCCCACAAACCGATTGCCACTCAACAGGCCCATCACTCCAGTGACAGCCCAttttt > 2:6589226/1‑137 (MQ=9) ggAGCGGGCGGGCCCGCGAAACAGGCGCCGCGGTGCATGGCACC‑GGCTTTTCCGGTG‑T‑TCG‑CGGG‑CAC‑TCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGcc < 2:9485577/137‑1 (MQ=21) gtgTGCCTTCGAAACGTGCGCCGTGGTGCGTGGCACT‑GTCTTTGCTGGTG‑T‑T‑CGTGGG‑CAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTaa < 1:2380521/135‑1 (MQ=19) cccGCGAAACAGGCGCCGTGGCGCATGGCACC‑GGCTTTGCCGGTG‑T‑TCG‑CGGG‑CAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAAt < 2:12603422/137‑1 (MQ=25) cgtgAAACAGGCGCCATGGTGCATGGCACC‑GGTTTTTCTGGTG‑T‑TCG‑TGGG‑CAT‑TCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCa < 2:8974696/134‑1 (MQ=255) cgcgAAACAGGCGCCGCGGTGCATGCCACC‑GGTTTTGCCGTTG‑T‑T‑CGCGGG‑CAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCTTCGCTAACGAATCa < 2:16155409/137‑1 (MQ=25) cgcgAAACAGCCTCCGCGGTGCATGCCACC‑GGTTTTGCTGTTG‑T‑TCG‑CGGC‑CAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCa < 1:4241438/137‑1 (MQ=255) cgcgAAAAAGGCGCCGGGTGGCATGGCACC‑GGTTTTGCCGTTG‑T‑TCG‑CGGT‑CAT‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGTCCGTTTTTTGTTGCCTCGCTAACGAATCa < 2:16563317/137‑1 (MQ=255) cgcaAAACGGGCGCTTCGGTGCATGGCACC‑GGTTTTGTTGGGG‑T‑TCG‑TGGG‑CAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCa < 2:18421284/137‑1 (MQ=25) cgagAAAAAGGCGTCGTGGTGCAGGGCACT‑GGTTTTGCTGGTG‑T‑TCG‑CGGG‑CAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCa < 1:16714405/134‑1 (MQ=25) agagAAACAGTCGCCGCGGTGCATGGCACT‑GGCTTTGCAGGTG‑T‑T‑CGCGGG‑CAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCa < 2:7872206/134‑1 (MQ=25) gcgAAACGGGCGCCGCGGTGCATGGCACC‑GGCTTTGCCGGTG‑T‑TCG‑CGGG‑CAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCAt < 2:13636591/137‑1 (MQ=25) gcgAAACAGGCGCCGCGGGGCATGGCACC‑GGCTTTGCCGGTG‑T‑T‑CGCGGG‑CAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCAt < 2:19466132/137‑1 (MQ=37) gagAAACAGGCGCCGCGGGGCATGGCACC‑GGCTTTGCCGGTG‑T‑TCG‑AGGG‑CAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCAt < 2:9210811/135‑1 (MQ=37) aaCAGGCGCCGCGGTGCATGGCACC‑GGCTTTGCCGGTG‑T‑TCG‑CGGG‑CAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCa > 1:540212/1‑132 (MQ=37) aaCAGGCGCCGCGGTGCATGGCACC‑GGCTTTGCCGGTG‑T‑T‑CGCGGG‑CAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCa < 2:540212/132‑1 (MQ=25) acggtcgccgcGGTGCATTGCACC‑GGTTTTGCTGGGG‑T‑TCG‑TGGG‑CAC‑CCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTc < 1:19440212/132‑1 (MQ=255) aCAGGCGCCGCGGTGCATGGCACC‑GGCTTTGCCGGTG‑T‑TCG‑CGGG‑CAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACta > 2:10908353/1‑136 (MQ=37) aCAGGCGCCGCGGTGCATGGCACC‑GGCTTTGCCGGTG‑T‑TCG‑CGGG‑CAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTc > 2:2124070/1‑137 (MQ=37) aCAGGCGCCGCGGTGCATGGCACC‑GGCTTTGCCGGTG‑T‑TCG‑CGGG‑CAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTc > 1:7424000/1‑137 (MQ=37) cgccgtGGGGCATGGCACT‑GGTTTTGCTGGTG‑T‑T‑C‑GGGGACAC‑CCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTa < 1:1905471/137‑1 (MQ=255) cgccgcGGTGCATGGCACC‑GGCTTTGCTGGTT‑TCC‑A‑CGGG‑CAC‑ACCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTa < 2:13934561/137‑1 (MQ=37) ctgcgGGGCAGGGCACC‑GGTTTTGCCGGTG‑T‑TCG‑AGGG‑CAC‑TCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGa < 1:16125544/135‑1 (MQ=255) ccgcGGTGCATGGCACC‑GGCTTTGCCGGTG‑T‑TCG‑CGGG‑CAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGa > 1:10129997/1‑137 (MQ=37) ggcacc‑GGCTTTGCCGGTG‑T‑TCG‑CGGG‑CAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAg > 1:3699995/6‑137 (MQ=37) acc‑GGCTTTGCCGGTG‑T‑TCG‑CGGG‑CAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAg > 2:5839534/3‑137 (MQ=38) acc‑GGCTTTGCCGGTG‑T‑TCG‑CGGG‑CAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAg > 2:5691744/3‑137 (MQ=38) c‑GGCTTTGCCGGTG‑T‑TCG‑CGGG‑CAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGAt > 2:11730762/1‑137 (MQ=38) c‑GGCTTTGCCGGTG‑T‑TCG‑CGGG‑CAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGAt > 1:7617038/1‑137 (MQ=38) ggCTTTGCCGGTG‑T‑TCG‑CGGG‑CAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATc > 1:12129885/1‑137 (MQ=38) cTTTGCCGGTG‑T‑TCG‑CGGG‑CAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAATGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTg > 2:8436614/1‑137 (MQ=38) cTTTGCCGGTG‑T‑TCG‑CGGG‑CAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCtt > 2:18932193/1‑115 (MQ=38) cTTTGCCGGTG‑T‑TCG‑CGGG‑CAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGCACGCTATCAAGCAGATCTg > 1:1231647/1‑137 (MQ=38) cTTTGCCGGTG‑T‑TCG‑CGGG‑CAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTg > 1:14072479/1‑137 (MQ=39) cTTTGCCGGTG‑T‑TCG‑CGGG‑CAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTg > 1:14747819/1‑137 (MQ=39) cTTTGCCGGTG‑T‑TCG‑CGGG‑CAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTg > 2:1515140/1‑137 (MQ=39) cTTTGCCGGTG‑T‑TCG‑CGGG‑CAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTg > 1:13438155/1‑137 (MQ=39) cTTTGCCGGTG‑T‑TCG‑CGGG‑CAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTg > 2:15959612/1‑137 (MQ=39) cTTTGCCGGTG‑T‑TCG‑CGGG‑CAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTg > 2:14936048/1‑137 (MQ=39) cTTTGCCGGTG‑T‑TCG‑CGGG‑CAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTg > 1:8721701/1‑137 (MQ=39) cTTTGCCGGTG‑T‑TCG‑CGGG‑CAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTg > 1:2849596/1‑137 (MQ=39) cTTTGCCGGTG‑T‑T‑CGCGGG‑CAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTg > 1:15344151/1‑137 (MQ=39) cTTTGCAGGTG‑T‑TCG‑CGGG‑CAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGTCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCACTTAGAACGCTATCAAGCAGATCTg > 2:17095145/1‑137 (MQ=38) tttGCCGGTG‑T‑TCG‑CGGG‑CAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGa > 1:5238019/1‑137 (MQ=39) ttGCCGGTG‑T‑TCG‑CGGG‑CAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGaa > 2:10649380/1‑137 (MQ=39) ttGCCGGTG‑T‑TCG‑CGGG‑CAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGaa > 1:18545355/1‑137 (MQ=39) ttGCCGGTG‑T‑TCG‑CGGG‑CAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGaa > 2:11982096/1‑137 (MQ=39) ttGCCGGTG‑T‑TCG‑CGGG‑CAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGaa > 2:12869465/1‑137 (MQ=39) ttGCCGGTG‑T‑TCG‑CGGG‑CAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACAAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGaa > 2:16670651/1‑137 (MQ=39) tgttcgcGGG‑CAC‑TCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTccc < 2:11222890/131‑1 (MQ=255) tcgcGGG‑CAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGAc > 1:10605428/4‑137 (MQ=255) cgcGGG‑CAC‑GCCCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGAct > 2:15337319/3‑137 (MQ=255) ||| TGAAGCCCGTGATGCGGCCATTCAGAGCCTGAACGAAGCCTTGGCACCACAGCAGGACTGAGGCTGCCAAACGGTGGGAGCGGGCGTGCCCGCGAAACAGGCGCCGCGGTGCATGGCCAC‑GGCTTTGCCGGTG‑T‑T‑T‑CGGG‑CAC‑G‑CCGCTCCCACAGACTGATTGCCATTAAGCGGGCCTATCGCTACAGTGACGGCCCGTTTTTTGTTGCCTCGCTAACGAATCATGACTCCCTTAGAACGCTATCAAGCAGATCTGAAACGTCCCGACT > AE015451_phaM1/1499363‑1499632 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |