BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	886,754	A→C	10.4%	V45G (GTT→GGT)	EO946_RS04490 ←	MFS transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	886,754	0	A	C	10.4%	451.0 / 7.2	188	V45G (GTT→GGT)	EO946_RS04490	MFS transporter
Reads supporting (aligned to +/- strand): ref base A (118/42); new base C (2/26); total (120/68)
Fisher's exact test for biased strand distribution p-value = 1.71e-11
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 192 total aligned reads displayed.

CCAAGTCCGAACAGGCAAACCTCAATGAGAAAAAGTTTCTTTCTGCCGTAGCGGTCGGAAAGCTTTCCGACGATCGGCACACTGACGCTAAGGCCGAGCGTATAGAGGGTAATGCCCCATGACCCCCAGGAAGGAGAAACGGCAA‑ACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCTTTTCTGCTCTGTCAATGCTTCAGACATATTC  >  NZ_CP034943/886616‑886891

ccACGTCCGAACAGGCAAACCTCAATGAGAAAAAGTTTCTTTCTGCCGTAGCGGTCGGAAAGCTTTCCGACGATCGGCACACTGACGCTCAGGCCGAGCGTATAGAGGGTAATGCCCCATGACCCCCCGGAAGGAGAAAc                                                                                                                                           >  2:74020/1‑140 (MQ=255)

ccAAGTCCGAACAGGCAAACCTCAATGAGAAAAAGTTTCTTTCTGCCGTAGCGGTCGGAACGCTTTCCGACGATCGGCACACTGACGCTAAGGCCGAGCGTCTAGAGGGTAATGCCCCATGACCCCCAGGAAGGAGAAAc                                                                                                                                           >  2:2443441/1‑140 (MQ=255)

        gAACAGGCAAACCTCAATGAGAAAAAGTTTCTTTCTGCCGTAGCGGTCGGAACGCTTTCCGACGATCGGCACACTGACGCTATGGCCGAGCGTATAGAGGGGAAGGCCCCATGTCCCCCCGGCAGGAGAAACGGCAA‑ACg                                                                                                                                  >  1:965026/1‑140 (MQ=255)

              gCACACCTCAATGAGAAAAAGTTTCTTTCTGCCGTAGCGGTCGGAAAGCTTTCCGACGATCGGCACACTGACGCTAGGGCCGAGCGTATAGAGGGTAATGCCCCATGACCCCCAGGAAGGTGAAACGGCAA‑ACGACTCGt                                                                                                                            >  1:2210745/1‑140 (MQ=255)

              gCAAACCTCAATGAGAAAAAGTTTCTTTCTGCCGTAGCGGTCGGAAAGCTTTCCGACGATCGGCACACTGACGCTAAGGCCGAGCGTCTAGAGGGTAATGCCCCATGACCCCCCGGAAGGAGAAACGGCAA‑ACGACTCgg                                                                                                                            >  1:2199735/1‑139 (MQ=255)

              gCAAACCTCAATGAGAAAAAGTTTCTTTCTGCCGTAGCGGTCGGAAAGCTTTCCGACGATCGGCACACTGACGCTAAGGCCGAGCGTATAGAGGGTAATGCCCCATGACCCCCAGGAAGGAGAAACGGCAA‑ACGACTCGt                                                                                                                            >  1:1402558/1‑140 (MQ=255)

              gCAAACCTCAATGAGAAAAAGTTTCTTTCTGCCGTAGCGGTCGGAAAGCTTTCCGACGATCGGCACACTGACGCTAAGGCCGAGCGTATAGAGGGTAATGCCCCATGACCCCCAGGAAGGAGAAACGGCAA‑ACGACTCGt                                                                                                                            >  2:671846/1‑140 (MQ=255)

                             aaaaaGTTTCTTTCTGCCGTAGCGGTCGGAAAGCTTTCCGACGATCGGCACACTGACGCTAAGGCCGAGCGTATAGAGGGTAATGCCCCATGACCCCCAGGAAGGAGAAACGGCAA‑ACGACTCGTTTATCGTTGTTAAag                                                                                                             >  2:4432553/1‑140 (MQ=255)

                             aaaaaGTTTCTTTCTGCCGTAGCGGTCGGAAAGCTTTCCGACGATCGGCACACTGACGCTAAGGCCGAGCGTATAGAGGGTAATGCCCCATGACCCCCAGGAAGGAGAAACGGCAA‑ACGACTCGTTTATCGTTGTTAAag                                                                                                             >  2:323482/1‑140 (MQ=255)

                                     tctttctGCCGTAGCGGTCGGCAAGCTTTCCGACGATAGGCCCACAGACCCTAAGGCCCAGCGTATAAAGTGTAATGACCCATGAACCCCAGGAACGAGAAACGGCAA‑AAGACTCGTTTATCGTTGTTAAAGCAGCAGaa                                                                                                     >  1:1620444/1‑140 (MQ=255)

                                         tgtGCCTTAGCGTTCGTAACGCTTTCCTACTTTCGGCACACTGACCAAAAGGCCTAGATTATAGTGGTTAATCCCCCATTACCCCCTGTAAGGACAACCGGCAA‑ACGACTCTTTTATCGTTTTTAAAGCAGCAGAaataa                                                                                                 <  2:2260139/138‑1 (MQ=255)

                                                        ttatcggtttCCTACGATCGTCAAACTGACGCTAAGCCCGATCGTATAGCGTGTAAGCCCCCATGACCCCCAGGAAGTACAAA‑GGCAACACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAgcg                                                                                  <  1:3359999/133‑1 (MQ=255)

                                                        ttaatggtttCCGACGATCGCCACACTGACGATAAGGCCGAGCTTATCGAGGTTACTGCCACATCACCCCCAGGAAGTAGAAACGCCAA‑ACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAgcg                                                                                  <  2:1563110/133‑1 (MQ=255)

                                                        gtatcgCTTTCCGCGGTTGGGAACACTGACGCTACGGCGGCGGTTATTGTGGGTATGGCCCCATCCCCCCCAGCACTGCGACAGGTAAC‑ACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAgcg                                                                                  <  1:2073206/135‑1 (MQ=255)

                                                        gtaacgCTTTCCGACTATCGGCACCCGGACGCTAAGGCCGAGCGTATTGAGGGTAATGCCCCATGACCCCCAGGAAGGAGAAACGGCAA‑ACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAgcg                                                                                  <  2:1242008/135‑1 (MQ=255)

                                                        gtaacgCTTCCCGACTATCGGAACACTCCCTCTAAGCCGGAGAGTATTGAGGTTAATCCCCCTTGACCCCCAGGACGCACAACCGGAAA‑ACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAgcg                                                                                  <  1:2449370/135‑1 (MQ=255)

                                                        ggAACGCTTTCCGACTATCGGCACCCTGCCGCTAAGGCCGAGCTTATAGCGGGTAATGCCCAATGACCCCCCGGAAGCATAAACGGCAA‑ACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAgcg                                                                                  <  2:806729/140‑1 (MQ=255)

                                                        gcaacggtttCCGACTTTCGGCACACTGCCGCTAAGGCCGAGCGTATAGTGGTTAATGCCCCATGACCCCCAGGACGGAGAAACGGAAA‑ACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAgcg                                                                                  <  2:2997617/133‑1 (MQ=255)

                                                        gcaacccTTGCCGACGATCGGAACCGTGCCGCTACGGCCTCGCTTTTCGCGGGTAAGGCCCCATGACCCCCCGGAAGCAGAAACGCCAC‑ACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAgcg                                                                                  <  1:4072270/134‑1 (MQ=255)

                                                        gacacgCTTCCCCACGCTCGGCACCCGCACCCTACGGCGGCGGGAATAGTGGTTAAGGCCCCTGCACCCCATGGACGCAGAAACGGCAA‑ACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAgcg                                                                                  <  1:4694182/135‑1 (MQ=255)

                                                           acccTTCCCCACGATCGCCACACTGCCGCTAAGGCCGAGGTTTAAGAGTGTAATCCCCCATCACCCCCAGCACGTACAACCGGCAA‑ACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAg                                                                               <  1:367708/137‑1 (MQ=255)

                                                            aGCTTTCCGACGATCGGCACACTGACTCTAAGGCCGAGCGTATAGAGGTTAATGCCCCATGACCCCCAGGAAGCAGAAACGGCAA‑ACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGc                                                                              <  2:4069374/140‑1 (MQ=255)

                                                                ttCCGACGATCGGCACACTGACGCTAAGGCCGAGCGTATAGAGGGTAATGCCCCATGACCCCCAGGAAGGAGAAACGGCAA‑ACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATa                                                                          >  1:1030999/1‑140 (MQ=255)

                                                                  ccGACGATGGGCACACTGACGCTAAGGCCGAGCGTATAGAGGTTAATGCCCCTTGACCCCCAGGAAGGAGAACCGGCAA‑ACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATaaa                                                                        <  1:4839506/140‑1 (MQ=255)

                                                                       gcacGGCACCGTGCCGCTACGGCCGTGGTTTTAGTGGTTTTTGCCCAAGGACCCCCAGGAGGGTTAACGGGCAC‑ACGACTAGTTTATCGTTGTTAAAGCAGCAGAAATAATTGGGTTATCCAGCGCAGCCATAAacacac                                                                   <  1:4040248/137‑1 (MQ=255)

                                                                       gaTGGGCACACGGACGCTACGGCCGAGGTTATAGCGGGTAATGCCCCATGACCCCCCGGAAGGAGAAACGGCAA‑ACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAacacac                                                                   <  1:817366/140‑1 (MQ=255)

                                                                       gaTGGGCAAACTGCCGCTACGGCCGAGCGTATAGTGGTTAATGCCCCATGACCCCAAGCAAGTAGAAACGTCAC‑ACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAacacac                                                                   <  2:2678642/140‑1 (MQ=255)

                                                                        aTCGGCACACTGACGCTAAGGCCGAGCGTATAGAGGGTAATGCCCCATGACCCCCAGGAAGGAGAAACGGCAA‑ACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACAcc                                                                  >  1:4510616/1‑140 (MQ=255)

                                                                                 cgtaCGCTAAGGCCGATCGTATAGAGGGTAATCCCCCATCCCCCCCAGCACGGAAAAACGGAAA‑ACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTaaa                                                         <  2:3233420/137‑1 (MQ=255)

                                                                                          aGGCCGAGCGTATAGAGGGTAATGCCCCATGACCCCCAGGAAGGAGAAACGGCAA‑ACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGAt                                                >  2:108902/1‑140 (MQ=255)

                                                                                          aGGCCGAGCGTATAGAGGGTAATGCCCCATGACCCCCAGGAAGGAGAAACGGCAA‑ACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGAt                                                >  2:121636/1‑140 (MQ=255)

                                                                                              gtagggTATAGAGGGTAATGCCCCAGCACCCCCAGGAAGGAGAAACGGAAA‑ACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGcc                                            <  2:4584997/135‑1 (MQ=255)

                                                                                              cgcgcttataGCGGGTACTGCCCCATCACCCCCCGGACGCCCAACCGGAAA‑ACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGcc                                            <  1:1569748/134‑1 (MQ=255)

                                                                                              cGAGTGTTTAGAGTGTAACGCCCCATGCCCCCCAGGACGGATACAGGGCAA‑ACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGcc                                            <  1:2817827/140‑1 (MQ=255)

                                                                                              cGAGCTTATAGTGGGTATTGCCCAATGACCCCCAGCAAGTAGAAACGGCAA‑ACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGcc                                            <  1:3398937/140‑1 (MQ=255)

                                                                                                aGCGTATAGAGGGTAATGCCCCATGACCCCCAGGAAGGAGAAACGGCAA‑ACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAacacac                                                                   >  1:1360596/1‑115 (MQ=255)

                                                                                                aGCGTATAGAGGGTAATGCCCCATGACCCCCAGGAAGGAGAAACGGCAA‑ACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCa                                          >  1:837709/1‑140 (MQ=255)

                                                                                                aGCGTATAGAGGGTAATGCCCCATGACCCCCAGGAAGGAGAAACGGCAA‑ACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCa                                          >  1:731413/1‑140 (MQ=255)

                                                                                                aGCGTATAGAGGGTAATGCCCCATGACCCCCAGGAAGGAGAAACGGCAA‑ACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCa                                          >  1:64346/1‑140 (MQ=255)

                                                                                                aGCGTATAGAGGGTAATGCCCCATGACCCCCAGGAAGGAGAAACGGCAA‑ACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCa                                          >  1:759876/1‑140 (MQ=255)

                                                                                                aGCGTATAGAGGGTAATGCCCCATGACCCCCAGGAAGGAGAAACGGCAA‑ACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCa                                          >  1:268950/1‑140 (MQ=255)

                                                                                                aGCGTATAGAGGGTAATGCCCCATGACCCCCAGGAAGGAGAAACGGCAA‑ACGACTCGTGTATCGGTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCAGAAACACACCAATCGTAAACAGGCTGATCGCCCa                                          >  2:4411177/1‑140 (MQ=255)

                                                                                                      taGAGGGTAATGCCCCATGACCCCCAGGAAGGAGAAACGGCAA‑ACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTg                                    >  1:689169/1‑140 (MQ=255)

                                                                                                      taGAGGGTAATGCCCCATGACCCCCAGGAAGGAGAAACGGCAA‑ACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTg                                    >  1:3763157/1‑140 (MQ=255)

                                                                                                       cggggtaaaTCCCCCATGACCCCCAGGACGGACAAACGCCAA‑ACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAAGTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTgg                                   <  1:1608234/133‑1 (MQ=255)

                                                                                                       cgagGGTAAGCCCCCCTGACCCCCAGGCAGTACAAACGCCAC‑ACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTgg                                   <  1:52698/139‑1 (MQ=255)

                                                                                                       agagGGTAAGGCCCCATGCCCCCCAGGACGGAGAACCGGCAC‑ACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTgg                                   <  1:3420186/140‑1 (MQ=255)

                                                                                                           ggTACTGCCCCATGACCCCCAGGAAGGAGAAACGGCAA‑ACGACTCGTGTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTGTTGGCttt                               >  1:3166953/1‑140 (MQ=255)

                                                                                                           ggTAATGCCCCATGACCCCCAGGAAGGAGAACCGGCAA‑ACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAgcg                                                                                  <  2:3163118/89‑1 (MQ=255)

                                                                                                           ggTAATGCCCCATGACCCCCAGGAAGGAGAAACGGCAA‑ACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCttt                               >  2:839680/1‑140 (MQ=255)

                                                                                                           ggTAATGCCCCATGACCCCCAGGAAGGAGAAACGGCAA‑ACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCttt                               >  2:1917193/1‑140 (MQ=255)

                                                                                                           ggTAATGCCCCATGACCCCCAGGAAGGAGAAACGGCAA‑ACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCttt                               >  2:2073342/1‑140 (MQ=255)

                                                                                                           ggTAATGCCCCATGACCCCCAGGAAGGAGAAACGGCAA‑ACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCttt                               >  2:1595793/1‑140 (MQ=255)

                                                                                                           ggTAATGCCCCATGACCCCCAGGAAGGAGAAACGGCAA‑ACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCttt                               >  2:3735914/1‑140 (MQ=255)

                                                                                                           ggTAATGCCCCATGACCCCCAGGAAGGAGAAACGGCAA‑ACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCttt                               >  2:1132483/1‑140 (MQ=255)

                                                                                                           ggTAATGCCCCATGACCCCCAGGAAGGAGAAACGGCAA‑ACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCttt                               >  2:3997547/1‑140 (MQ=255)

                                                                                                           ggTAATGCCCCATGACCCCCAGGAAGGAGAAACGGCAA‑ACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCttt                               >  2:4797283/1‑140 (MQ=255)

                                                                                                           ggTAATGCCCCATGACCCCCAGGAAGGAGAAACGGCAA‑ACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCttt                               >  2:4933983/1‑140 (MQ=255)

                                                                                                           ggTAATGCCCCATGACCCCCAGGAAGGAGAAACGGCAA‑ACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCAAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCttt                               >  1:3506443/1‑140 (MQ=255)

                                                                                                           ggTAATGCCCCATGACCCCCAGGAAGGAGAAACGGCAA‑ACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCAGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACATGCTGATCGCCCACTGTTGGCttt                               >  1:3692038/1‑140 (MQ=255)

                                                                                                           ggTAATGCCCCATGACCCCCAGGAAGGAGAAACGGCAA‑ACGACTCGTGTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTACACAGGCTGATCGCCCACTTTTGGCttt                               >  1:1158149/1‑140 (MQ=255)

                                                                                                              aaTGCCCCATGACCCCCAGGAAGGAGAAACGGCAA‑ACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCTTTTCt                            >  1:986123/1‑140 (MQ=255)

                                                                                                              aaTGCCCCATGACCCCCAGGAAGGAGAAACGGCAA‑ACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCTTTTCt                            >  1:1696387/1‑140 (MQ=255)

                                                                                                              aaTGCCCCATGACCCCCAGGAAGGAGAAACGGCAA‑ACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCTTTTCt                            >  2:4663645/1‑140 (MQ=255)

                                                                                                              aaTGCCCCATGACCCCCAGGAAGGAGAAACGGCAA‑ACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCTTTTCt                            >  1:4657901/1‑140 (MQ=255)

                                                                                                               aTGCCCCATGACCCCCAGGAAGGAGAAACGGCAC‑AAGACTCGTTTGTCGTTGTTAAAGCGGCAGACATAAGTCCGTGATCCAGCGCAGGCATATACACCCGAATAGTAAACAGGCTGATCGCCCACGTGTGGCTTGTCTg                           >  1:2407442/1‑140 (MQ=255)

                                                                                                               aTGCCCCATGACCCCCAGGAAGGAGAAACGGCAA‑ACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCAGAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCTTTTCTg                           >  1:3726774/1‑140 (MQ=255)

                                                                                                               aTGCCCCATCACCCCCAGCAAGAAGAAACGCCAA‑ACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCTTTTCTg                           <  1:4331660/140‑1 (MQ=255)

                                                                                                                tGCCCCATGACCCCCAGGAAGTATAAACGGCAA‑ACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCTTTTCTGc                          <  1:1026810/140‑1 (MQ=255)

                                                                                                                     cATGACCCCCAGGAAGGAGAAACGGCAA‑ACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATc                                                                                       >  1:1763624/1‑74 (MQ=255)

                                                                                                                     cATGACCCCCAGGAAGGAGAAACGGCAA‑ACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCTTTTCTGCTCTGt                     >  1:4129353/1‑140 (MQ=255)

                                                                                                                     cATGACCCCCAGGAAGGAGAAACGGCAA‑ACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCTTTTCTGCTCTGt                     >  1:4308490/1‑140 (MQ=255)

                                                                                                                     cATGACCCCCAGGAAGGAGAAACGGCAA‑ACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCTTTTCTGCTCTGt                     >  1:4642361/1‑140 (MQ=255)

                                                                                                                     cATGACCCCCAGGAAGGAGAAACGGCAA‑ACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCTTTTCTGCTCTGt                     >  1:2977520/1‑140 (MQ=255)

                                                                                                                     cATGACCCCCAGGAAGGAGAAACGGCAA‑ACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCTTTTCTGCTATGt                     >  2:40985/1‑140 (MQ=255)

                                                                                                                       tGACCCCCAGGAAGGATAAACGGCAA‑ACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCTTTTCTGCTCTGTCa                   <  2:1369689/140‑1 (MQ=255)

                                                                                                                        gACCCCCCGTACGGACAAACGGAAC‑ACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCTTTTCTGCTCTGTCaa                  <  1:3553683/140‑1 (MQ=255)

                                                                                                                        gACCCCCAGTAAGCCGAACCGCAAA‑ACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCTTTTCTGCTCTGTCaa                  <  2:2572232/140‑1 (MQ=255)

                                                                                                                        gACCCCCAGTAAGCACAACCCCCAA‑ACGACTCGTTTATCGTTGTTAAAGCAGCACAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCTTTTCTGCTCTGTCaa                  <  2:3839941/140‑1 (MQ=255)

                                                                                                                        gACCCCCAGGACGGACAACCGGCAA‑ACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCTTTTCTGCTCTGTCaa                  <  1:2581867/140‑1 (MQ=255)

                                                                                                                        ccccccccGGCAGCACAAAGGGCAC‑ACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCTTTTCTGCTCTGTCaa                  <  2:217522/138‑1 (MQ=255)

                                                                                                                        caCCCCCAGGAAGGTGAAACGCCAA‑ACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCTTTTCTGCTCTGTCaa                  <  2:341578/139‑1 (MQ=255)

                                                                                                                           ccccCGGCAGGAGAAACGGCAA‑ACGACTCGTTTATCGGTGTTAAAGCAGCAGACATAATTCCGTTATCCAGCGCCGCCCTAATCACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCTGTTCTGCTCTGTCAATGc               >  2:3699119/1‑140 (MQ=255)

                                                                                                                           ccccAGGAAGGAGAAACGGCAA‑ACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCTTTTCTGCTCTGTCAATGc               >  2:1643090/1‑140 (MQ=255)

                                                                                                                             ccAGGAAGGAGAAACGGCAA‑ACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCCCTTTTGGCTTTTCTGCTCTGTCAATGCtt             >  1:907168/1‑140 (MQ=255)

                                                                                                                             ccAGGAAGGAGAAACGGCAA‑ACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCTTTTCTGCTCTGTCAATGCtt             >  1:2648548/1‑140 (MQ=255)

                                                                                                                               aggacggaGAAACGGCAA‑ACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCTTTTCTGCTCTGTCAATGCTTCa           <  2:2837101/140‑1 (MQ=255)

                                                                                                                                     gagaAACGGCAA‑ACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGcc                                                                             <  1:1113211/68‑1 (MQ=255)

                                                                                                                                     gagaAACGGCAA‑ACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGc                                                                              >  2:2030519/1‑67 (MQ=255)

                                                                                                                                     gagaAACGGCAA‑ACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCTTTTCTGCTCTGTCAATGCTTCAGACata     >  1:1854271/1‑140 (MQ=255)

                                                                                                                                     gagaAACGGCAA‑ACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCTTTTCTGCTCTGTCAATGCTTCAGACata     >  2:158617/1‑140 (MQ=255)

                                                                                                                                     gagaAACGGCAA‑ACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCTTTTCTGCTCTGTCAATGCTTCAGACata     >  1:1695647/1‑140 (MQ=255)

                                                                                                                                     gagaAACGGCAA‑ACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCTGTTCTGCTCTGTCACTGCTTCAGACata     >  2:2649652/1‑140 (MQ=255)

                                                                                                                                     cacaaaGGGCAA‑ACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCTTTTCTGCTCTGTCAATGCTTCAGACata     <  1:4412574/137‑1 (MQ=255)

                                                                                                                                      agaAACGGCAA‑ACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCTTTTCTGCTCTGTCAATGCTTCAGACatat    >  1:4206123/1‑140 (MQ=255)

                                                                                                                                      agaAACGGCAA‑ACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCTTTTCTGCTCTGTCAATGCTTCAGACatat    >  1:783470/1‑140 (MQ=255)

                                                                                                                                      agaAACGGCAA‑ACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCTTTTCTGCTCTGTCAATGCTTCAGACatat    >  1:1529368/1‑140 (MQ=255)

                                                                                                                                      agaAACGGCAA‑ACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCTTTTCTGCTCTGTCAATGCTTCAGACatat    <  2:1854271/140‑1 (MQ=255)

                                                                                                                                        aaaCGGCAA‑ACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCTTTTCTGCTCTGTCAATGCTTCAGACATAttc  >  2:3648469/1‑140 (MQ=255)

                                                                                                                                        aaaCGGCAA‑ACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCTTTTCTGCTCTGTCAATGCTTCAGACATAttc  >  2:2720623/1‑140 (MQ=255)

CCAAGTCCGAACAGGCAAACCTCAATGAGAAAAAGTTTCTTTCTGCCGTAGCGGTCGGAAAGCTTTCCGACGATCGGCACACTGACGCTAAGGCCGAGCGTATAGAGGGTAATGCCCCATGACCCCCAGGAAGGAGAAACGGCAA‑ACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCTTTTCTGCTCTGTCAATGCTTCAGACATATTC  >  NZ_CP034943/886616‑886891

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`