BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	1,514,935	G→T	5.5%	Q44K (CAA→AAA)	EO946_RS07920 ←	YlaI family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	1,514,935	0	G	T	5.5%	691.1 / 14.4	241	Q44K (CAA→AAA)	EO946_RS07920	YlaI family protein
Reads supporting (aligned to +/- strand): ref base G (31/193); new base T (15/2); total (46/195)
Fisher's exact test for biased strand distribution p-value = 2.01e-10
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 247 total aligned reads displayed.

AAAAGACTCAGCTGAGAAGCTGAGTCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTC‑TTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGCACATATATGTATGGATTGGGCGGTTTCGAAGCCGTTTCGCGATCAGGGTATCATCATCAATTGTTTCAATTTTATCGCAAATAGAACATTGGACCCTCATCATATCACC  >  NZ_CP034943/1514796‑1515070

aaaaGACTCAGCTGAGAAGCTGAGACTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTC‑TTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTg                                                                                                                                         <  1:1810722/140‑1 (MQ=255)

  aaGACTCAGCTGAGAAGCTGAGTCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTC‑TTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTggg                                                                                                                                       <  1:2806985/140‑1 (MQ=255)

  aaGACTCAGCTGAGAAGCTGAGTCTTTTTTTGTGTTATGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTC‑TTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTggg                                                                                                                                       <  1:3813675/140‑1 (MQ=255)

  aaGACTCAGCTGAGAAGCTGAGTCTTATTTTGTGTATTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTCTTTC‑TTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTggg                                                                                                                                       <  1:4516661/140‑1 (MQ=255)

   aGACTCAGCTGAGAAGCTGAGTCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTC‑TTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGc                                                                                                                                      >  2:3535541/1‑140 (MQ=255)

       tCAGCTGAGAAGCTGAGTCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTC‑TTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATc                                                                                                                                  <  1:1248160/140‑1 (MQ=255)

         aGCTGAGAAGCTGAGTCTTTTGTTGTGTTTTGTATGGTAAAGCGGCCGCTGTATTTTTTGTCTTTTTC‑TTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCg                                                                                                                                >  2:2186527/1‑140 (MQ=255)

                 aGCTGAGTCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTC‑TTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGc                                                                                                                        <  2:2241533/140‑1 (MQ=255)

                 aGCTGAGTCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTC‑TTTTTTTTGCTGTCCCGGGTGAAAAGTGAATGTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGc                                                                                                                        <  1:194559/140‑1 (MQ=255)

                   cTGAGTCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTTACTTTTTG‑TTTTTTTTTCTTTCCCGGGTGAAAAGTGGATTTTCCCGTTTTAAGCCGTTCTTCTGTTTTTTTGGCAATAAGGTCGTGGCAt                                                                                                                      >  1:2292476/1‑140 (MQ=255)

                   cTGAGTCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTG‑TTTTTTTTTCTGTCCCGGGTGAAAAGTGAATTTTCACGTTTTAATCCGTTATTATTTTTTTTGGGAAATACGGTCGTGGCAt                                                                                                                      >  2:4479281/1‑140 (MQ=255)

                   cTGAGTCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTA‑TTTTTTTTGCTGTACCGGGTGAAAAGTTAATTTTCCCGTTTTAAGCCGGTCATATGTTTTTTGTGCAAACAGGTCGTTGCAt                                                                                                                      >  2:2686773/1‑140 (MQ=255)

                   cTGAGTCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCTTTAATTTTTTGCTTTTTC‑TTTTTTTTGCTGTCCCGGGTGAAAAGTTAATTTTCCCGGTTTCAGCCGTTCTTATTTTTTTTGGGCAATCCGGTCGTTGCAt                                                                                                                      >  2:350950/1‑140 (MQ=255)

                   cTGAGTCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCGTTTTTTTTTTTCTTTTTG‑TTTTTTTTTGTGTGCCCGGTGTAAAGTGAATTTTTGGGTTTTGAGCCGGTGTTCTGTTTTTTTGGGAATGCGGtggtggggt                                                                                                                      >  1:2594446/1‑137 (MQ=255)

                     gAGTCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTC‑TTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTc                                                                                                                    <  2:1085690/140‑1 (MQ=255)

                     gAGTCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTC‑TTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTAAGCCGTTCTTATGTTTTTTTGGCAATCCGGTCGTGGCAtta                                                                                                                    >  2:2005245/1‑139 (MQ=255)

                      agccTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTC‑TTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTcc                                                                                                                   <  2:1930590/137‑1 (MQ=255)

                      aGTCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTCTTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTc                                                                                                                    <  2:1192297/140‑1 (MQ=255)

                        tCTTTTTTTGTTTTTTGTTTGGTAAACCGGCCGATTTATTTTTTGACTTTTTC‑TTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTg                                                                                                                 <  2:1677750/140‑1 (MQ=255)

                        tCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTC‑TTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTg                                                                                                                 <  1:498617/140‑1 (MQ=255)

                         cTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTC‑TTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTgg                                                                                                                <  1:2686773/140‑1 (MQ=255)

                         cTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTC‑TTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTgg                                                                                                                <  2:686750/140‑1 (MQ=255)

                         cTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTC‑TTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTgg                                                                                                                <  2:3802165/140‑1 (MQ=255)

                         cTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTC‑TTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTgg                                                                                                                <  1:4859646/140‑1 (MQ=255)

                          tttttttGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTC‑‑TTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGca                                                                                                              <  2:4575011/140‑1 (MQ=255)

                           ttttttGTGTTTTGTATGGTAACGCGGCCGCTTTATTTTTTGACTTTTTC‑TTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGca                                                                                                              <  1:4237624/140‑1 (MQ=255)

                           ttttttGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTC‑TTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGca                                                                                                              <  2:4016135/140‑1 (MQ=255)

                           ttttttGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTC‑TTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGca                                                                                                              <  1:3676649/140‑1 (MQ=255)

                           ttttttGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTC‑TTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCCTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGca                                                                                                              <  2:437638/140‑1 (MQ=255)

                           ttttttGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTC‑TTTTTTTTGATGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGca                                                                                                              <  1:72182/140‑1 (MQ=255)

                           tattttGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTC‑TTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGca                                                                                                              <  2:819304/138‑1 (MQ=255)

                             ttttGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTC‑TTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGcaca                                                                                                            <  2:1443649/140‑1 (MQ=255)

                             ttttGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTGTTC‑TTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGGTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGcaca                                                                                                            <  2:4664278/140‑1 (MQ=255)

                              tttGTGTTTTGTATGGTAAAGAGGCCTCTTTATTTTTTTACTTTTTC‑TTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCATTTTTCAGCAGTTCTTCTGTTTTTTGGGCAATGCGGTCGTGGCATTCCTGGCACat                                                                                                           <  1:636908/140‑1 (MQ=255)

                              tttGTGTTTTGTATGGGACAGCGGCCGCTTTATTTTTTGACTTTTTC‑TTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTGTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGCACat                                                                                                           <  1:2989507/140‑1 (MQ=255)

                               ttGTGTTTTGTATGGTACAGCGGCCGCTTTATTTTTTGACTTTTTC‑TTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGCACata                                                                                                          <  1:3308309/140‑1 (MQ=255)

                               ttGTGTTTTGTATGGTAACGCGGCCGCTTTATTTATTGACTTTGTC‑TTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGCACata                                                                                                          <  1:4406457/140‑1 (MQ=255)

                               ttGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTC‑TTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGCACata                                                                                                          <  1:683515/140‑1 (MQ=255)

                               ttGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTC‑TTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGCACata                                                                                                          <  2:4937451/140‑1 (MQ=255)

                               ttGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTC‑TTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGCACata                                                                                                          <  2:3604484/140‑1 (MQ=255)

                               ttGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTC‑TTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGCACata                                                                                                          <  1:22383/140‑1 (MQ=255)

                               ttGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTC‑TTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGCACata                                                                                                          <  2:2653264/140‑1 (MQ=255)

                               gtgtgTTTTGTATGGTACAGCGGCCGCTTTATTTTTTGACTTTTTC‑TTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGCACata                                                                                                          <  2:4884472/139‑1 (MQ=255)

                                tgtgTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTC‑TTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGCACatat                                                                                                         <  2:122899/140‑1 (MQ=255)

                                tgtgTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTC‑TTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGCACatat                                                                                                         <  2:1539661/140‑1 (MQ=255)

                                tgtgTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTC‑TTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGCACatat                                                                                                         <  2:2148246/140‑1 (MQ=255)

                                    ttttGTATGGTAAAGCGGCCGCGTTATTTTTTTACTTTTTC‑TTTTTTTTTCTTTCACCGGTGAAAAATGAAATTTAACCTTTTAAGCCCTTATTATTTTTTTTTGTGAAAACGGTAGTTGCATTACATGCACaaataatt                                                                                                     >  1:4652102/1‑137 (MQ=255)

                                     tttGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTC‑TTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGCACATAtatgta                                                                                                    <  2:1230844/140‑1 (MQ=255)

                                              tAAAGCGGCCGCTTTATTTTTTGCCTTTTTC‑TTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGCACATATATGTATGGATTggg                                                                                           <  2:1164254/140‑1 (MQ=255)

                                              tAAAGCGGCCGCTTTATTTTTTGACTTTTTC‑TTTTTTTTGCTGTCCCGGGTGACAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGCACATATATGTATGGATTggg                                                                                           <  2:34703/140‑1 (MQ=255)

                                              tAAAGCGGCCGCTTTATTTTTTGACTTTTTC‑TTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGCACATATATGTATGGATTggg                                                                                           <  2:229526/140‑1 (MQ=255)

                                              tAAAGCGGCCGCTTTATTTTTTGACTTTTTC‑TTTTTTGTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGCACATATATGTATGGATTggg                                                                                           <  2:4927677/140‑1 (MQ=255)

                                              tAAAGCGGCCGCTATATTTTTTGACTTTTTC‑TTTTTTTTGCTGTCCCGGGTGAAAAGTGAAGTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGCACATATATGTATGGATTggg                                                                                           <  2:3326870/140‑1 (MQ=255)

                                               aaaGCGGCCGCTTTATTTTTTGACTTTTTC‑TTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGGCGTGGCATTCCTGGCACATATATGTATGGATTGGGc                                                                                          <  2:3980120/140‑1 (MQ=255)

                                                   cGGCCGCTTTATTTTTTGACTTTTTC‑TTTTTTTTGCTTTCCCGGGTGAAAAGTGAATTTTACCGTTTTCAGCCGTTCTTCTGTTTTTTTGGCAATCCGGTCGTGGCATTCCGGGCACCTATATGTATGGATTTGGCGGtt                                                                                      >  2:2377079/1‑140 (MQ=255)

                                                                    gACTTTTTC‑‑TTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGCACATATATGTATGGATTGGGCGGTTTCGAAGCCGTTTCGCGAt                                                                    <  2:282893/140‑1 (MQ=255)

                                                                     cctttttc‑tttttTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGCACATATATGTATGGATTGGGCGGTTTCGAAGCCGTTTCGCGAt                                                                    <  2:1867946/139‑1 (MQ=255)

                                                                     aCTTTTTC‑TTTTTTTTGCTGTCCCGGGTGAACAGTGAATTTTGCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCTTTCATGGCACATATATGTCTGGATTGGGCGGTTTCGAAGCCGTTTCGCGAt                                                                    <  1:2039777/140‑1 (MQ=255)

                                                                     aCTTTTTC‑TTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGCACATATATGTATGGATTGGGCGGTTTCGAAGCCTTTTCGCGAt                                                                    <  1:1078867/140‑1 (MQ=255)

                                                                     aCTTTTTC‑TTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGCACATATATGTATGGATTGGGCGGTTTCGAAGCCGTTTCGCGAt                                                                    <  2:3125722/140‑1 (MQ=255)

                                                                     aCTTTTTC‑TTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGCACATATATGTATGGATTGGGCGGTTTCGAAGCCGTTTCGCGAt                                                                    <  1:358324/140‑1 (MQ=255)

                                                                     aCTTTTTC‑TTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGCACATATATGTATGGATTGGGCGGTTTCGAAGCCGTTTCGCGAt                                                                    <  1:3138244/140‑1 (MQ=255)

                                                                     aCTTTTTC‑TTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGCACATATATGTATGGATTGGGCGGTTTCGAAGCCGTTTCGCGAt                                                                    <  1:2438865/140‑1 (MQ=255)

                                                                     aCTTTTTC‑TTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGCACATATATGTATGGATTGGGCGGTTTCGAAGCCGTTTCGCGAt                                                                    <  2:1762585/140‑1 (MQ=255)

                                                                     aCTTTTTC‑TTTTTTTTGCTGTCCCGGGTGAAAAGTGAATGTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGCACATATATGTATGGATTGGGCGGTTTCGAAGCCGTTTCGCGAt                                                                    <  2:1043852/140‑1 (MQ=255)

                                                                     aCTTTTTC‑TTTTTTGTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGCACATATATGTATGGATTGGGCGGTTTCGAAGCCGTTTCGCGAt                                                                    <  1:349401/140‑1 (MQ=255)

                                                                     aCTTTTTC‑TTTTTTCTGCTGTCCCGGGTGAAAAGTAAATTTTCCCGTTTTCAGCCGTTCCTCTGTTTTTTTGGCAATCCGATCGGGGCATTCCTGGCACATATATGGATGGATCGGGCGGTTTCGAAGCCGTTTCGCGAt                                                                    <  2:154944/140‑1 (MQ=255)

                                                                               tttttttGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGCACATATATGTATGGATTGGGCGGTTTCGAAGCCGTTTCGCGATCAGGGTatc                                                           <  1:1477791/140‑1 (MQ=255)

                                                                               tttttttGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGCACATATATGTATGGATTGGGCGGTTTCGAAGCCGTTTCGCGATCAGGGTatc                                                           <  2:3046979/140‑1 (MQ=255)

                                                                               tttttttGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGCACATATATGTATGGATTGGGCGGTTTCGAAGCCGTTTCGCGATCAGGGTatc                                                           <  1:2005245/140‑1 (MQ=255)

                                                                                                    aaaGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGCACata                                                                                                          >  2:2769832/1‑72 (MQ=255)

                                                                                                    aaaGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGCACATATATGTATGGATTGGGCGGTTTCGAAGCCGTTTCGCGATCAGGGTATCATCATCAATTGTTTCAAtttt                                      >  1:98995/1‑140 (MQ=255)

                                                                                                         gAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGCACATATATGTATGGATTGGGCGGTTTCGAAGCCGTTTCGCGATCAGGGTATCATCATCAATTGTTTCAATTTTATCGc                                 <  1:2186527/140‑1 (MQ=255)

                                                                                                              ttCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGCACATATATGTATGGATTGGGCGGTTTCGAAGCCGTTTCGCGATCAGGGTATCATCATCAATTGTTTCAATTTTATCGCAAATa                            <  1:1038901/140‑1 (MQ=255)

                                                                                                              ttCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGCACATATATGTATGGATTGGGCGGTTTCGAAGCCGTTTCGCGATCAGGGTATCATCATCAATTGTTTCAATTTTATCGCAAATa                            <  1:1619905/140‑1 (MQ=255)

                                                                                                               tCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGCACATATATGTATGGATTGGGCGGTTTCGAAGCCGTTTCGCGATCAGGGTATCATCATCAATTGTTTCAATTTTATCGCAAATAg                           <  2:922400/140‑1 (MQ=255)

                                                                                                               tCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGCACATATATGTATGGATTGGGCGGTTTCGAAGCCGTTTCGCGATCAGGGTATCATCATCAATTGTTTCAATTTTATCGCAAATAg                           <  1:2352608/140‑1 (MQ=255)

                                                                                                               tCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGCACATATATGTATGGATTGGGCGGTTTCGAAGCCGTTTCGCGATCAGGGTATCATCATCAATTGTTTCAATTTTATCGCAAATAg                           <  2:622486/140‑1 (MQ=255)

                                                                                                               tCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGCACATATATGTATGGATTGGGCGGTTTCGAAGCCGTTTCGCGATCAGGGTATCATCATCAATTGTTTCAATTTTATCGCAAATAg                           <  2:2090216/140‑1 (MQ=255)

                                                                                                               tCCCGTTTTCAGCCGTTCTTCTGGTTTTTGGGCAATCCGGTCGTGGCATTCCTGGCACATATATGTATGGATTGGGCGGTTTCGAAGCCGTTTCGCGATCAGGGTATCATCATCAATTGTTTCAAGTTTATCGCAAATAg                           <  2:2580412/140‑1 (MQ=255)

                                                                                                                cccGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGCACATATATGTATGGATTGGGCGGTTTCGAAGCCGTTTCGCGATCAGGGTATCATCATCAATTGTTTCAATTTTATCGCAAATAGa                          <  2:2028581/140‑1 (MQ=255)

                                                                                                                  cGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGCACATATATGTATGGATTGGGCGGTTTCGAAGCCGTTTCGCGATCAGGGTATCATCATCAATTGTTTCAATTTTATCGCAAATAGAAc                        >  1:811654/1‑140 (MQ=255)

                                                                                                                    ttttCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGCACATATATGTATGGATTGGGCGGTTTCGAAGCCGTTTCGCGATCAGGGTATCATCATCAATTGTTTCAATTTTATCGCAAATAGAACAt                      >  2:4131189/1‑140 (MQ=255)

                                                                                                                      ttCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGCACATATATGTATGGATTGGGCGGTTTCGAAGCCGTTTCGCGATCAGGGTATCATCATCAATTGTTTCAATTTTATCGCAAATAGAACATTg                    <  2:4564745/140‑1 (MQ=255)

                                                                                                                      ttCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGCACATATATGTATGGATTGGGCGGTTTCGAAGCCGTTTCGCGATCAGGGTATCATCATCAATTGTTTCAATTTTATCGCAAATAGAACATTg                    <  2:2222069/140‑1 (MQ=255)

                                                                                                                      ttCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGCACATATATGTATGGATTGGGCGGTTTCGAAGCCGTTTCGCGATCAGGGTATCATCATCAATTGTTTCAATTTTATCGCAAATAGAACATTg                    <  2:3411893/140‑1 (MQ=255)

                                                                                                                          gCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGCACATATATGTATGGATTGGGCGGTTTCGAAGCCGTTTCGCGATCAGGGTATCATCATCAATTGTTTCAATTTTATCGCAAATAGAACATTGGAcc                <  2:2881561/140‑1 (MQ=255)

                                                                                                                          gCCGTTCTTCTGTTTTGTGGGCAATCCGGTCGTGGCATTCCTGGCACATAGATGTATGGATTGGGCGGTTTCGAAGCCGTTTCGCGATCAGGGTATCATCATCAATTGTTTCACTTTTATCGCAAATAGAACATTGGAcc                <  1:4931245/140‑1 (MQ=255)

                                                                                                                           ccGTTC‑TCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGCACATATATGTATGGATTGGGCGGTTTCGAAGCCGTTTCGCGATCAGGGTATCATCATCAATTGTTTCAATTTTATCGCAAATAGAACATTGGACCCt              <  2:4647751/140‑1 (MQ=255)

                                                                                                                            cGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGCACATATATGTATGGATTGGGCGGTTTCGAAGCCGTTTCGCGATCAGGGTATCATCATCAATTGTTTCAATTTTATCGCAAATAGAACATTGGACCCt              <  2:1102643/140‑1 (MQ=255)

                                                                                                                            cGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGCACATATATGTATGGATTGGGCGGTTTCGAAGCCGTTTCGCGATCAGGGTATCATCATCAATTGTTTCAATTTTATCGCAAATAGAACATTGGACCCt              <  2:3435777/140‑1 (MQ=255)

                                                                                                                            agTTCTTGTGTTTTTTGGGCAATCCGGGCGTGTCATTCCTGGCACATATATGTATGGATTGGGCGGTTTCGAAGCCGTTTCGCGAGCAGGGTATCATCATCAATTGTTTCAATTTTATCGCAAATAGAACATTGGACCCt              <  2:4109761/139‑1 (MQ=255)

                                                                                                                               tcttctGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGCACATATCTGTATGGATTGGGCGGTTTCGAAGCCGTTTCGCGATCAGGGTATCATCATCAATTGTTTCAATTTTATCGCAAATAGAACATTGGACCCtcat           <  2:3140724/140‑1 (MQ=255)

                                                                                                                               tcttctGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGCACATATATGTATGGATTGGGCGGTTTCGAAGCCGTTTCGCGATCAGGGTATCATCATCAATTGTTTCAATTTTATCGCAAATAGAACATTGGACCCtcat           <  2:3668930/140‑1 (MQ=255)

                                                                                                                                     gTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGCACATATATGTATGGATTGGGCGGTTTCGAAGCCGTTTCGCGATCAGGGTATCATCATCAATTGTTTCAATTTTATCGCAAATAGAACATTGGAcc                <  2:1260789/129‑1 (MQ=255)

                                                                                                                                      ttttttGGGCAATCCGGTCGTGGCATTCCTGGCACATATATGTATGGATTGGGCGGTTTCGAAGCCGTTTCGCGATCAGGGTATCATCATCAATTGTTTCAATTTTATCGCAAATAGAACATTGGACCCTCATCATATCa    <  2:555032/140‑1 (MQ=255)

                                                                                                                                       tttttGGGCAATCCGGTCGTGGCATTCCTGGCACATATATGTATGGATTGGGCGGTTTCGAAGCCGTTTCGCGATCAGGGTATCATCATCAATTGTTTCAATTTTATCGCAAATAGAACATTGGACCCTCATCATATCAc   <  2:4272052/140‑1 (MQ=255)

                                                                                                                                       tttttGGGCAATCCGGTCGTGGCATTCCTGGCACATATATGTATGGATTGGGCGGTTTCGAAGCCGTTTCGCGATCAGGGTATCATCATCAATTGTTTCAATTTTATCGCAAATAGAACATTGGACCCTCATCATATCAc   <  2:2321498/140‑1 (MQ=255)

                                                                                                                                        ttttGGGCAATCCGGTCGTGGCATTCCTGGCACATATATGTATGGATTGGGCGGTTTCGAAGCCGTTTCGCGATCAGGGTATCATCATCAATTGTTTCAATTTTATCGCAAATAGAACATTGGACCCTCATCATATCAcc  <  2:3799451/140‑1 (MQ=255)

                                                                                                                                        tagtGGGCAATCCCGTCGTGGCATTCCTTGCACATATATGTATGGATTGGGCGGTTTCGAAGACGTTTCGAGATCAGGTTATCATCATCAATTGTTTCAATTTTATCGCAAATAGAACATTGGACCCTCATCATATCAcc  <  2:2515545/137‑1 (MQ=255)

AAAAGACTCAGCTGAGAAGCTGAGTCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTTTTC‑TTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGCACATATATGTATGGATTGGGCGGTTTCGAAGCCGTTTCGCGATCAGGGTATCATCATCAATTGTTTCAATTTTATCGCAAATAGAACATTGGACCCTCATCATATCACC  >  NZ_CP034943/1514796‑1515070

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`