BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	1,968,161	G→T	17.6%	D78Y (GAT→TAT)	EO946_RS09780 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	1,968,161	0	G	T	17.6%	200.6 / 7.1	104	D78Y (GAT→TAT)	EO946_RS09780	hypothetical protein
Reads supporting (aligned to +/- strand): ref base G (27/55); new base T (20/2); total (47/57)
Fisher's exact test for biased strand distribution p-value = 1.03e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 113 total aligned reads displayed.

CTTCTGCAAAAGAAGTAAACCCGAATGTAAATATCTTTCTCAATTCAGCTGTAGAAAATTTGAACCCTGATGACGTTAAACAAACCCTAAAGACAATTAAAGAGTCGATTATCTTAAAAAACGGTGAATATTCATTTGATGAATCGAAAGCAATAAAACTAGGGCTTGAGAAAGAT‑GTAATTGATAACGTTAAGAACCTAGAATCTAATGTCTTAGAGCAATTCTACAATCTTAAAGATAGTGAAATTGAAACAGGCTCAGAGCAATTACAAGGAGA  >  NZ_CP034943/1968024‑1968300

cTTCTGCAAAAGAAGTAAACCCTAATGTAAATATCTTTCTCAATTCAGCTGTAGAAAATTTGAACCCTGATGACGTTAAACAAACCCTAAAGACAATTAAAGAGTCGATTATCTTAAAAAACGGTGAATATTCATTtgat                                                                                                                                            <  2:3803471/140‑1 (MQ=255)

cTTCTGCAAAAGAAGTAAACCCGAATGTAAATATCTTTCTCAATTCAGCTGTAGAAAATTTGAACCCTGATGACGTTAAACAAACCCTAAAGACAATTAAAGAGTCGATTATCTTAAAAAACGGTGAATATTCATTtgat                                                                                                                                            >  1:1754435/1‑140 (MQ=255)

cTTCTGCAAAAGAAGTAAACCCGAATGTAAATATCTTTCTCAATTCAGCTGTAGAAAATTTGAACCCTGATGACGTTAAACAAACCCTAAAGACAATTAAAGAGTCGATTATCTTAAAAAACGGTGAATATTCATTtgat                                                                                                                                            >  2:3311298/1‑140 (MQ=255)

  tCTGCAAAAGAAGTAAACCCGAATGTAAATATCTTTCTCAATTCAGCTGTAGAAAATTTGAACCCTGATGACGTTAAACAAACCCTAAAGACAATTAAAGAGTCGAGTTTCTTAAAAAACGGTGAATATTAATTttatga                                                                                                                                          >  1:2001038/1‑140 (MQ=255)

    tGCAAAAGAAGTAAACCCGAATGTAAATATCTTTCTCAATTCAGCTGTAGAAAATTTGAACCCTGATGACGTTAAACAAACCCTAAAGACAATTAAAGATTCGATTATCTTAAAAAACGGTGAATATTGATTTGATTAAt                                                                                                                                        >  2:4932977/1‑140 (MQ=255)

    tGCAAAAGAAGTAAACCCGAATGTAAATATCTTTCTCAATTCAGCTGTAGAAAATTTGAACCCTGATGACGTTAAACAAACCCTAAAGACAATTAAAGAGTCGATTATGTTTAAAAAAGGTGAATATTAATTttattatt                                                                                                                                        >  2:3093324/1‑136 (MQ=255)

    tGCAAAAGAAGTAAACCCGAATGTAAATATCTTTCTCAATTCAGCTGTAGAAAATTTGAACCCTGATGACGTTAAACAAACCCTAAAGACAATTAAAGAGTCGATTATGTTAAAAAACGGTGCATATTCATTTGATGAAt                                                                                                                                        >  2:601105/1‑140 (MQ=255)

    tGCAAAAGAAGTAAACCCGAATGTAAATATCTTTCTCAATTCAGCTGTAGAAAATTTGAACCCTGATGACGTTAAACAAACCCTAAAGACAATTAAAGAGTCGATTATCTTTAAAAACGGTGAAAATTCATTTTATTAAt                                                                                                                                        >  1:2961183/1‑140 (MQ=255)

    tGCAAAAGAAGTAAACCCGAATGTAAATATCTTTCTCAATTCAGCTGTAGAAAATTTGAACCCTGATGACGTTAAACAAACCCTAAAGACAATTAAAGAGTCGATTATCTTAAAAAACGGTGAATATTGATTttatttat                                                                                                                                        >  1:2893808/1‑136 (MQ=255)

    tGCAAAAGAAGTAAACCCGAATGTAAATATCTTTCTCAATTCAGCTGTAGAAAATTTGAACCCTGATGACGTTAAACAAACCCTAAAGACAATTAAAGAGTCGATTATCTTAAAAAACGGGGAATATTAATTTTATTAAt                                                                                                                                        >  2:347576/1‑140 (MQ=255)

    tGCAAAAGAAGTAAACCCGAATGTAAATATCTTTCTCAATTCAGCTGTAGAAAATTTGAACCCTGATGACGTTAAACAAACCCTAAAGACAATTAAAGAGTCGAGTATCTTAAAAAACGGGGAAAATTGATTTTATGTAt                                                                                                                                        >  2:4285806/1‑140 (MQ=255)

    tGCAAAAGAAGTAAACCCGAATGTAAATATCTTTCTCAATTCAGCTGTAGAAAATTTGAACCCTGATGACGTTAAACAAACCCTAAAGACAATTAAAGAGTCGAGTATAGTAAAAAACGGGGAATATTGAGTtgatgtaa                                                                                                                                        >  1:2658771/1‑137 (MQ=255)

        aaaGAAGTAAACCCGAATGTAAATATCTTTCTCAATTCAGCTGTAGAAAATTTGAACCCTGATGACGTTAAACAAACCCTAAAGACAATTAAAGAGTCGATTATCTTAAAAAACGGTGAATATTCATTTGATGAATCGaa                                                                                                                                    <  2:1182773/140‑1 (MQ=255)

        aaaGAAGTAAACCCGAATGTAAATATCTTTCTCAATTCAGCTGTAGAAAATTTGAACCCTGATGACGTTAAACAAACCCTAAAGACAATTAAAGAGTCGATTATCTTAAAAAACGGTGAATATTCATTTGATGAATCGaa                                                                                                                                    <  2:722369/140‑1 (MQ=255)

        aaaGAAGTAAACCCGAATGTAAATATCTTTCTCAATTCAGCTGTAGAAAATTTGAACCCTGATGACGTTAAACAAACCCTAAAGACAATTAAAGAGTCGATTATCTTAAAAAACGGTGAATATTCATTTGATGAATCGaa                                                                                                                                    <  2:4951624/140‑1 (MQ=255)

        aaaGAAGTAAACCCGAATGTAAATATCTTTCTCAATTCAGCTGTAGAAAATTTGAACCCTGATGACGTTAAACAAACCCTAAAGACAATTAAAGAGTCGATTATCTTAAAAAACGGTGAATATTCATTTGATGAATCGaa                                                                                                                                    <  2:1754435/140‑1 (MQ=255)

             agTAAACCCGAATGTAAATATCTTTCTCAATTCAGCTGTAGAAAATTTGAACCCTGATGACGTTAAACAAACCCTAAAGACAATTAAAGAGTCGATTATCTTAAAAAACGGTGAATATTCATTTGATGAATCGAAAGCaa                                                                                                                               <  2:1934103/140‑1 (MQ=255)

             agTAAACCCGAATGTAAATATCTTTCTCAATTCAGCTGTAGAAAATTTGAACCCTGATGACGTTAAACAAACCCTAAAGACAATTAAAGAGTCGATTATCTTAAAAAACGGTGAATATTCATTTGATGAATCGAAAGCaa                                                                                                                               <  2:4115944/140‑1 (MQ=255)

              ttAAACCCGAATGTAAATATCTTTCTCAATTCAGCTGTAGAAAATTTGAACCCTGATGACGTTAAACAAACCCTAAAGACAATTAAAGAGTCGATTATCTTAAAAAACGGTGAATATTCATTTGATGAATCGAAAGCAAt                                                                                                                              <  1:4386076/139‑1 (MQ=255)

              ttAAACCCGAATGTAAATATCTTTCTCAATTCAGCTGTAGAAAATTTGAACCCTGATGACGTTAAACAAACCCTAAAGACAATTAAAGAGTCGATTATCTTAAAAAACGGTGAATATTCATTTGATGAATCGAAAGCAAt                                                                                                                              <  1:3091566/139‑1 (MQ=255)

              gTAAACCCGAATCTAAATATCTTTCTCAATTCAGCTGTAGAAAATTTGAACCCTGATGACGTTACACAAACCCTACAGACAATTAAAGAGTCGATTATCTTAAAAAACGGTGAATATTCATTTGATGAATCGAAAGCAAt                                                                                                                              <  1:3012461/140‑1 (MQ=255)

                    ccGAATGTAAATATCTTTCTCAATTCAGCTGTAGAAAATTTGAACCCTGATGACGTTAAACAAACCCTAAAGACAATTAAAGAGTCGATTATCTTAAAAAACGGTGAATATTCATTTGATGAATCGAAAGCAATAAAACt                                                                                                                        <  1:3387370/140‑1 (MQ=255)

                       aaTGTAAATATCTTTCTCAATTCAGCTGTAGAAAATTTGAACCCTGATGACGTTAAACAAACCCTAAAGACAATTAAAGAGTCGATTATGTTATAAAACGGTGAATATTGATTTGATGTATCGAAAGCAATaaaaagatg                                                                                                                     >  2:466935/1‑135 (MQ=255)

                         tGTAAATATCTTTCTCAATTCAGCTGTAGAAAATTTGAACCCTGATGACGTTAAACAAACCCTAAAGACAATTAAAGAGTCGATTATCTTAAAAAAAGGTGAATATTGATTTGGTGTATCGAAAGCAATTAAACTAtggg                                                                                                                   >  2:1218765/1‑139 (MQ=255)

                              ctatctatcTCAATTCAGCTCTAGAAAAATAGCACCCTGACGACGAGAAACCACCCCTAAACCCACTTAACGAGTCGATTATCTTAAAAAACGGTGAATATTCATTTGATGAATCGAAAGCAATAAAACTAGGGCTTgag                                                                                                              <  1:236702/139‑1 (MQ=255)

                              atatatTTCTCAATTCAGCTTTAGAAAATTTGAACCCTGATGACGTTAAACAAACCCTAAAGACAATTAAAGAGTCGATTATCTTAAAAAACGGTGAATATTCATTTGATGAATCGAAAGCAATAAAACTAGGGCTTgag                                                                                                              <  1:148403/140‑1 (MQ=255)

                              atatCTTTCTCAATTCAGCTGTAGAAAATTTGAACCCTGATGACGTTAAACAAACCCTAAAGACAATTAAAGAGTCGATTATCTTAAAAAACGGTGAATATTCATTTGATGAATCGAAAGCAATAAAACTAGGGCTTgag                                                                                                              <  1:4317308/140‑1 (MQ=255)

                               tatCTTTCTCAATTCAGCTGTATAAAATTTGAACCCTGATGACGTTATACAAACCCTAAAGACAATTAAAGAGTCGATTATCTTAAAAAACGGTGAATATTCATTTTATGAATCGAAAGCAATAAAACTAGGGCTTgaga                                                                                                             <  2:3902663/140‑1 (MQ=255)

                               tatCTTTCTCAATTCAGCTGTAGACAATTTGAACCCTGATGAAGTTAAACAAACCCTAACGACAATTAAAGAGTCGATGATCTTAAAAAACGGTGAATATTCATTGGATGAATCGAAAGCAATAAAACTAGGGCTTgaga                                                                                                             <  2:3901866/140‑1 (MQ=255)

                               tatCTTTCTCAATTCAGCTGTAGAAAATTTGAACCCTGATGACGTTAAACAAACCCTAAAGACAATTAAAGAGTCGATTATCTTAAAAAACGGTGAATATTCATTTGATGAATCGAAAGCAATAAAACTAGGGCTTgaga                                                                                                             <  2:2314108/140‑1 (MQ=255)

                                atCTTTCTCAATTCAGCTGTAGAAAATTTGAACCCTGATGACGTTAAACAAACCCTAAAGACAATTAAAGAGTCGATTATCTTAAAAAACGGTGAATATTCATTTGATGAATCGAAAGCAATAAAACTAGGGCTTGAGaa                                                                                                            <  1:1785714/140‑1 (MQ=255)

                                  cTTTCTCAATTCAGCTGTAGAAAATTTGAACCCTGATGACGTTAAACAAACCCTAAAGACAATTAAAGAGTCGATTATCTTAACAAACGGTGAATATTCATTTGATGAATAGAAAGCAATAAAACTAGGGGGTGGGAAAg                                                                                                          >  2:1405358/1‑140 (MQ=255)

                                  cTTTCTCAATTCAGCTGTAGAAAATTTGAACCCTGATGACGTTAAACAAACCCTAAAGACAATTAAAGAGTCGATTATCTTAAAAAACGGTGAATATTGATTTGATTAATCGAAATCAAAAAAAATATGGCGTGAGAAAg                                                                                                          >  2:1257826/1‑140 (MQ=255)

                                  cTTTCTCAATTCAGCTGTAGAAAATTTGAACCCTGATGACGTTAAACAAACCCTAAAGACAATTAAAGAGTCGAGTATATTAAAAAAAGGTGAATATTGATTTGATGAATCGAAAGGAATAAAACTAGGGGTTGAGAAAg                                                                                                          >  1:2729153/1‑140 (MQ=255)

                                     tctcAATTCAGCTGTAGAAAATTTGAACCCTGATGACGTTAAACAAACCCTAAAGACAATTAAAGAGTCGATTATCTTAAAAAACGGTGAATATTCATTTTATGAATCGAAAGCAATAAAAAAAGGGCTTTAGAAAAAtt                                                                                                       >  1:1710275/1‑139 (MQ=255)

                                     tctcAATTCAGCTGTAGAAAATTTGAACCCTGATGACGTTAAACAAACCCTAAAGACAATTAAAGAGTCGATTATCTTAAAAAACCGTGTATATTCATTTGTTGAATAGAAAGCAATAAAACTAGGGCTTGTGAAAGAtt                                                                                                       >  1:60852/1‑139 (MQ=255)

                                     tctcAATTCAGCTGTAGAAAATTTGAACCCTGATGACGTTAAACAAACCCTAAAGACAATTAAAGAGTCGATTATCTTAAAAAACCGTGAAAATTCATTTTATGAATAGAAAGCAAAAAAAATAGGGCTTGAGAAAAAtt                                                                                                       >  1:3071117/1‑139 (MQ=255)

                                     tctcAATTCAGCTGTAGAAAATTTGAACCCTGATGACGTTAAACAAACCCTAAAGACAATTAAAGAGTCGATTATCTTAAAAAAAGGTGTATATTCATTTTATGAATCGAAAGCAATAAAAATAGGGCGTGAGGAatatt                                                                                                       >  1:3925960/1‑139 (MQ=255)

                                     tctcAATTCAGCTGTAGAAAATTTGAACCCTGATGACGTTAAACAAACCCTAAAGACAATTAAAGAGTCGAGTTTCTTAAAAAAAGGTGAATATTGATTTGATGAATAGAAAGGAATAAAACAAGGGGGTGAGAAAGAtt                                                                                                       >  1:374754/1‑139 (MQ=255)

                                     tctcAATTCAGCTGTAGAAAATTTGAACCCTGATGACGTTAAACAAACCCTAAAGACAATTAAAGAGTCGAGTATCTTAAAAAAAGGTGAATATTAATTTGGTGAATCGAAAGCAATAAAAATAGGGCTTGAGAAAGAT‑g                                                                                                      >  1:2154277/1‑140 (MQ=255)

                                           ttCAGCTGTAGAAAATTTGAACCCTGATGACGTTAAACAAACCCTAAAGACAATTAAAGAGTCGATTATCTTTAAAAACGGGGAAAATTCAGTTGATGAAAAGAAACCAATAAAACTAGGGCTTTACAAAAAT‑GTAATTg                                                                                                >  2:2465558/1‑140 (MQ=255)

                                           ttCAGCTGTAGAAAATTTGAACCCTGATGACGTTAAACAAACCCTAAAGACAATTAAAGAGTCGAGTAACTTTAAAAAAGGGGAATATTGATATGTTGTATAGAAATAAAAAAAAATGGGGGTTGAGGAAGCT‑GTTATTg                                                                                                >  2:2506032/1‑140 (MQ=255)

                                             cAGCTGTAGAAAATTTGAACCCTGATGACGTTAAACAAACCCTAAAGACAATTAAAGAGTCGATTATCTTAAAAAACGGTGAATATTCATTTGATGAATCGAAAGCAATAAAACTAGGGCTTGAGAAAGAT‑GTAATTGAt                                                                                              <  1:1969346/140‑1 (MQ=255)

                                              aGCTGTAGAAAATTTGAACCCTGATGACGTTAAACAAACCCTAAAGACAATTAAAGAGTCGATTATCTTAAAAAACGGTGAATATTCATTTGATGAATCGAAAGCAATAAAACTAGGGCTTGAGAAAGAT‑GTAATTGATa                                                                                             <  1:270479/140‑1 (MQ=255)

                                                     gAAAATTTGAACCCTGATGACGTTAAACAAACCCTAAAGACAATTAAAGAGTCGATTATCTTAAAAAACGGTGAATATTAATTTGATGAAACGAAAGCAATAAAAATAGGGCGTGAGAAAGAT‑GTAATTTATAACGTTaa                                                                                      >  2:1545421/1‑140 (MQ=255)

                                                     gAAAATTTGAACCCTGATGACGTTAAACAAACCCTAAAGACAATTAAAGAGTCGATTATCTTAAAAAACGGTGAATATTAAATTTATTAATCGAAATAAATAAAAATAGGGCTTTAGAAAGAT‑GTAATTGATAACGTTaa                                                                                      >  2:4173187/1‑140 (MQ=255)

                                                     gAAAATTTGAACCCTGATGACGTTAAACAAACCCTAAAGACAATTAAAGAGTCGATTATCTTAAAAAACGGTGAAAATTCATTTTATGAATCGAAAGCAATAAAACAAGGGCTTGAGAAAAAT‑GTAATTTATAACGATaa                                                                                      >  2:2396851/1‑140 (MQ=255)

                                                     gAAAATTTGAACCCTGATGACGTTAAACAAACCCTAAAGACAATTAAAGAGTCGATTATCTTAAAAAACAGTGAATAATAATTTTATTAAAAGAAAGCAAAAAAAATAGTGCGTGAGACAGAT‑TTAATTTATAAACTTaa                                                                                      >  2:2598637/1‑140 (MQ=255)

                                                          tttGAACCCTGATGACGTTAAACAAACCCTAAAGACAATTAAAGAGTCGATTATATTAAAAAAAGGTGAAAATTCATTTGATGGATCGAAAGCAAAAAAACTATGGCGTGAGAAAGAT‑TTAATTGATAAACTTAAGaaac                                                                                 >  1:4594443/1‑138 (MQ=255)

                                                          tttGAACCCTGATGACGTTAAACAAACCCTAAAGACAATTAAAGAGTCGAGTATCTTAAAAAACGGGGGAAATTCAGTTTAAGAATCCAAAGCAAAAAAAAGACGGCGTGTGTAAAAT‑GTAAGTGATAACGTTAAGAAcc                                                                                 >  1:4260771/1‑140 (MQ=255)

                                                          tttGAACCCTGATGACGTTAAACAAACCCTAAAGACAATTAAAGAGTCGAGTATCTTAAAAAACCGTGAATATTGATTTGATGAATCGAAAGCCAAAAAAATAGGGCGTGGGGAAGAT‑GTGATTGATAAAGTTAAGAAcc                                                                                 >  1:3304378/1‑140 (MQ=255)

                                                          tttGAACCCTGATGACGTTAAACAAACCCTAAAGACAATTAAAGAGTCGAGTATCTTAAAAAAAGGTGAATATTAATTTTATGAATCGAAAGCAAAAAAACTAGGGCTTGATAAACAT‑GTAATTGATAACCTTAATAAcc                                                                                 >  1:3851044/1‑140 (MQ=255)

                                                          tttGAACCCTGATGACGTTAAACAAACCCTAAAGACAATTAAAGAGTCGAGTATATTAAAAAACGGTGAAAAATAATTTGATGTAAAGAAAACAATAAAACAAGGGGATGAGAAACAT‑TTAAATTATAAACTTAATAAca                                                                                 >  1:2748516/1‑139 (MQ=255)

                                                          tttGAACCCTGATGACGGTAAACAAACCCTAAAGACAATTAAAGAGTCGATTATCTTAAAAAACGGGGAAAATTCATTTTTTGAATAGAAAGCAAAAAAACTAGTGCGTGAGAAAGAT‑GTAATTTATAAAGTTaattacc                                                                                 >  1:4379789/1‑135 (MQ=255)

                                                            tGAACCCTGATGACGTTAAACAAACCCTAAAGACAATTAAAGAGTCGATTATCTTAAAAAACGGTGAATATTCATTTGATGAATCGAAAGCAATAAAACTAGGGCTTGAGAAAGAT‑GTAATTGATAACGTTAAGAACCTa                                                                               <  2:58752/140‑1 (MQ=255)

                                                            tGAACCCTGATGACGTTAAACAAACCCTAAAGACAATTAAAGAGTCGATTATCTTAAAAAACGGTGAATATTCATTTGATGAATCGAAAGCAATAAAACTAGGGCTTGAGAAAGAT‑GTAATTGATAACGTTAAGAACCTa                                                                               <  2:425970/140‑1 (MQ=255)

                                                             gAACCCTGATGACGTTAAACAAACCCTAAAGACAATTAAAGAGTCGATTATCTTAAAAAACGGTGAATATTCATTTGATTAATCGAAAGCAATAAAACTAGGGCGTGAGAAAGAT‑TTAATTGATAACGTTAAGAACCTAg                                                                              >  1:2757526/1‑140 (MQ=255)

                                                             gAACCCTGATGACGTTAAACAAACCCTAAAGACAATTAAAGAGTCGAGTATCTTAAAAAACGGTGAAAATTAATTTTATGAATAGAAAGCAAAAAAAATAGGGCGTGTGAAAGAT‑TTAATTGATAACGTTGAAAAACTAg                                                                              >  1:3366421/1‑140 (MQ=255)

                                                             gAACCCTGATGACGTTAAACAAACCCTAAAGACAATTAAAGAGTCGAGTATCTTAAAAAACACTAAATAATCAATTTATGAATCGAAAGCAATAAAAATAGGGGTGGAGAAAAAT‑TTAATTGATTACGTTAAGAACCGAg                                                                              >  1:158535/1‑140 (MQ=255)

                                                               aaccTGATGACGTTAAACAAACCCTAAATACAATTAAAGAGTCGATTATCTTAAAAAACGGTGAATATTCATTTGATGAATCGAAAGCAATAAAACTAGGGCTTGAGAAAGAT‑GTAATTGATAACGTTAAGAACCTAGaa                                                                            <  2:4472106/138‑1 (MQ=255)

                                                               aCCCTGATGACGTTAAACAAACCCTAAAGACAATTAAAGAGTCGATTATCTTAAAAAACGGTGAATATTCATTTGATGAATCGAAAGCAATAAAACTAGGGCTTGAGAAAGAT‑GTAATTGATAACGTTAAGAACCTAGaa                                                                            <  2:766154/140‑1 (MQ=255)

                                                                cccTGATGACGTTAAACAAACCCTAAAGACAATTAAAGAGTCGATTTTGTTAAAAAAAGGTGTATATTCAATTGATGTATCGAAAGCAAAAAAACAAGGGCTTGAGAAAGAT‑TTAATTTTTAACGTTAAGAACCaataat                                                                           >  1:2421610/1‑140 (MQ=255)

                                                                   tgatgaCGTTAAACAAACCCTAACGACAATTAAAGAGTCGATTATCTTAAAAAACGGTGAATATTCATTTGATGAATCGAAAGCAATAAAACTAGGGCTTGAGAAAGAT‑GTAATTGATAACGTTAAGAACCTAGAATCTa                                                                        <  2:1130865/140‑1 (MQ=255)

                                                                   tgatgaCGTTAAACAAACCCTAAAGACAATTAAAGAGTCGATTATCTTAAAAAACGGTGAATATTCATTTGATGAATCGAAAGCAATAAAACTAGGGCTTGAGAAAGAT‑GTAATTGATAACGTTAAGAACCTAGAATCTa                                                                        <  2:3412123/140‑1 (MQ=255)

                                                                   tgatgaCGTTAAACAAACCCTAAAGACAATTAAAGAGTCGATTATCTTAAAAAACGGTGAATATTCATTTGATGAATCGAAAGCAATAAAACTAGGGCTTGAGAAAGAT‑GTAATTGATAACGTTAAGAACCTAGAATCTa                                                                        <  2:2620564/140‑1 (MQ=255)

                                                                   tgatgaCGTTAAACAAACCCTAAAGACAATTAAAGAGTCGATTATCTTAAAAAACGGTGAATATTCATTTGATGAATCGAAAGCAATAAAACTAGGGCTTGAGAAAGAT‑GTAATTGATAACGTTAAGAACCTAGAATCTa                                                                        <  2:4763300/140‑1 (MQ=255)

                                                                   tgatgaCGTTAAACAAACCCTAAAGACAAATAAAGAGTCGATTATCTTAAAAAACGGTGAATATTCATTTGATGAATCGAAAGCAATAAAACTAGGGCTTGAGAAAGAT‑GTAATTGATAACGTTAAGAACCTAGAATCTa                                                                        <  2:1187422/140‑1 (MQ=255)

                                                                   tgatgaCGTTAAACAAACCCTAAAGAAAATTAAAGAGTCGATTATCTTAAAAAACGGTGAATATTCATTTGATGAATCGAAAGCAATAAAACTAGGGCTTGAGAAAGAT‑GTAATTGATAACGTTAAGAACCTAGAATCTa                                                                        <  2:1649690/140‑1 (MQ=255)

                                                                   tgaggaCGTTAAACAAACCCTAAACACAATTAAAGAGTCGATTATCTTAAAAAACGGTGAATAGTCATTTGATGAATCGAAAGCAATAAAACTAGGGCTTGAGAAAGAT‑GTAATTGATAACGTTAAGAACCTAGAATCTa                                                                        <  2:1457094/140‑1 (MQ=255)

                                                                    gatgaCGTTAAACAAACCCTAAAGACAATTAAAGAGTCGATTTTGTTAAAAAAAGGTGAATATTAATTTGTTTAATCGAAAACAATAAAACTAGGGCTTTAGTAAGAT‑TTAATTGATAACGTTAACAACCGAGaaaaaaa                                                                       >  1:2459146/1‑135 (MQ=255)

                                                                            tAAACAAACCCTAAAGACAATTAAAGAGTCGATTATCTTAAAAAACGGTGAATATTCATTTGATGAATCGAAAGCAATAAAACTAGGGCTTGAGAAAGAT‑GTAATTGATAACGTTAAGAACCTAGAATCTAATGTCTTag                                                               <  2:4688774/140‑1 (MQ=255)

                                                                              aacaaacCCTAAAGACAATTAAAGAGTCGATTATCTTAAAAAACGGTGAATATTCATTTGATGAATCGAAAGCAATAAAACTAGGGCTTGAGAAAGAT‑GTAATTGATAACGTTAAGAACCTAGAATCTAATGTCTTagag                                                             <  1:3643024/140‑1 (MQ=255)

                                                                              aacaaacCCTAAAGACAATTAAAGAGTCGATTATCTTAAAAAACGGTGAATATTCATTTGATGAATCGAAAGCAATAAAACTAGGGCTTGAGAAAGAT‑GTAATTGATAACGTTAAGAACCTAGAATCTAATGTCTTagag                                                             <  1:4284342/140‑1 (MQ=255)

                                                                                   acCCTAAAGACAATTAAAGAGTCGATTGTCTTAAAAAAAGGTGAATATTAAATTGTTGAATGGAAAGCCAAAAAACAAGGGCGTGAGTAAGATGGTAATT‑ATAACGGTAAAAAACTAGAATCTAAAGTCTTTGAGCAAtt                                                        >  1:3417920/1‑140 (MQ=255)

                                                                                   acCCTAAAGACAATTAAAGAGTCGATTATCTTAAAAAACGGGGAATAATCATTTTGTGAATCGAAAGCAATAAAACTAGGGGTTGAGAAATAT‑TTAAATGATAAAGTTAAGAACCTAGAATCAAAATTCTTAGAGCAAtt                                                        >  2:3764211/1‑140 (MQ=255)

                                                                                   acCCTAAAGACAATTAAAGAGTCGATTATCTTAAAAAAAGGTGAATATTAATTTGATTAATAGAAAGCAATAAAAATAGGGCTTTAGAAAGAT‑GTAATTTATAACGTTAAGAACCTATAATCTAATTTCTTAGAGCAAtt                                                        >  2:3763657/1‑140 (MQ=255)

                                                                                      cTAAAGACAATTAAAGAGTCGATTATCTTAAAAAACGGTGAATATTCATTTGATGAATCGAAAGCAATAAAACTAGGGCTTGAGAAAGAT‑GTAATTGATAACGTTAAGAACCTAGAATCTAATGTCTTAGAGCAATTCTa                                                     <  2:1842117/140‑1 (MQ=255)

                                                                                      cTAAAGACAATTAAAGAGTCGATTATCTTAAAAAACGGTGAATATTCATTTGATGAATCGAAAGCAATAAAACTAGGGCTTGAGAAAGAT‑GTAATTGATAACGTTAAGAACCTAGAATCTAATGTCTTAGAGCAATTCTa                                                     <  2:2001038/140‑1 (MQ=255)

                                                                                      cTAAAGACAATTAAAGAGTCGATTATCTTAAAAAACGGTGAATATTCATTTGATGAATCGAAAGCAATAAAACTAGGGCTTGAGAAAGAT‑GTAATTGATAACGTTAAGAACCTAGAATCTAATGTCTTAGAGCAATTCTa                                                     <  1:379595/140‑1 (MQ=255)

                                                                                        aaaGACAATTAAAGAGTCGATTAAAATAAAAAAACGTGAAAAATCATTTTATGAAAAGAAACAAAAAAAACAAGGGGTTTAGAAAGAT‑GTAAATGATAACCTTAACAAACAAGAATATAATGTCTTAGAGCAATtataaa                                                   >  2:3490895/1‑138 (MQ=255)

                                                                                               aTTAAAGAGTCGAGTATCTTAAAAAACCGTGAAAATTCATTTTATGAATCGAAAGCCATAAAACAAGGGCGGGAGAAAGAA‑GTAATTGATAACGTTAAGAACCTAGAATCTAATGTATTATAGCAAATATACAATCTTaa                                            >  2:4467137/1‑140 (MQ=255)

                                                                                                        tCGAGTATCTTAAAAAACGGTGAATATTAATTTTATGAATAGAAAGCAATAAAACAAGGGGTTTAGGAAGAT‑GTAATTGATAACGGTAATAACCTATAATATAATGTCTTTGAGCAAATCTACAAAATTAAAGATAGTGa                                   >  2:2435433/1‑140 (MQ=255)

                                                                                                            ttATCTTAAAAAACGGTGATTATTCATTTGATGTATCGAAAGCAATAAAACTAGGGCTTGATAAAGAT‑GTAATTGATAACGTTAAGAACCTAGAATCTTATGTCTTAGAGCAATTCTACAATCTTAAAGATAGTGAAAtt                               <  1:3490895/140‑1 (MQ=255)

                                                                                                                 ttAAAAAACGGTGAATATTCATTTGATGAATCGAAAGCAATAAAACTAGGGCTTGAGAAAGAT‑GTAATTGATAACGTTAAGAACCTAGAATCTAATGTCTTAGAGCAATTCTACAATCTTAAAGATAGTGAAATTGAAAc                          <  1:466935/140‑1 (MQ=255)

                                                                                                                 ttAAAAAACGGTGAATATTCATTGGATGAATCGAAAGCAATAAAACTAGGGCTTGAGAAAGAT‑GTAATTGATAACGTTAAGAACCTAGAATCTAATGTCTTAGAGCAATTCTACAATCTTAAAGATAGTGAAATTGAAAc                          <  1:4598185/140‑1 (MQ=255)

                                                                                                                  tAAAAAACGGTGAATATTCATTTGATGAATCGAAAGCAATAAAACTAGGGCTTGAGAAAGAT‑GTAATTGATAACGTTAAGAACCTAGAATCTAATGTCTTAGAGCAATTCTACAATCTTAAAGATAGTGAAATTGAAACa                         >  1:4573969/1‑140 (MQ=255)

                                                                                                                  tAAAAAACGGTGAATATTCATTTGATGAATCGAAAGCAATAAAACTAGGGCTTGAGAAAGAT‑GTAATTGATAACGTTAAGAACCTAGAATCTAATGTCTTAGAGCAATTATACAATCTTAAAGATAGTGAAATTGAAACa                         >  1:1831779/1‑140 (MQ=255)

                                                                                                                  tAAAAAACGGTGAATATTCATTTGATGAATCGAAAGCAATAAAACTAGGGCTTGAGAAAGAT‑GTAATTGATAACGTTAAGAACCTAGAATCTAATGTCTTAGAGAAATTCTACAATCTTAAACATAGTGAAATTGAAACa                         >  1:2702299/1‑140 (MQ=255)

                                                                                                                      aaaCGGTGACTATTCATTTGATGAATCGAAAGCAATAAAACGAGGGCTTGAGAAAGAT‑GTAATTGATAACGTTAAGAACCTAGAATCTAAGGTCTTAGAGCAATTCTACAATCTTAAAGATAGTGAAATTGAAACAGGCt                     <  1:4936795/140‑1 (MQ=255)

                                                                                                                      aaaCGGTGAATATTCATTTTATTAATCGAAAGCAATATAACTATGTCTTTAGAAAGAT‑GTAATTGATAACTTTAAGAACCTAGAATCTAATGTCTTAGAGCAATTCTACAATCTTAAAGATAGTTTAATTGAAACAGGCt                     <  1:2804161/140‑1 (MQ=255)

                                                                                                                      aaaCGGTGAATATTCATTTGATGACTCGAAAGCAATAAAACTAGGGCTTGAGAAAGAT‑GTAATTGATAACGTTAAGAACCTAGAATCTAATGTCTTAGAGCAATTCTACAATCTTAAAGATAGTGAAATTGAAACAGGCt                     <  2:4145160/140‑1 (MQ=255)

                                                                                                                      aaaCGGTGAATATTCATTTGATGAATCGAAAGCAATAAAACTAGGGCTTGAGAAAGAT‑GTAATTGATAACGTTAAGAACCTAGAATCTAATGTCTTAGAGCAATTCTACAATCTTAAAGATAGTGAAATTGAAACAGGCt                     <  1:519533/140‑1 (MQ=255)

                                                                                                                      aaaCGGTGAATATTCATTTGATGAATCGAAAGCAATAAAACTAGGGCTTGAGAAAGAT‑GTAATTGATAACGTTAAGAACCTAGAATCTAATGTCTTAGAGCAATTCTACAATCTTAAAGATAGTGAAATTGAAACAGGCt                     <  2:1710275/140‑1 (MQ=255)

                                                                                                                      aaaCGGTGAATATTCATTTGATGAATCGAAAGCAATAAAACTAGGGCTTGAGAAAGAT‑GTAATTGATAACGTTAAGAACCTAGAATCTAATGTCTTAGAGCAATTCTACAATCTTAAAGATAGTGAAATTGAAACAGGCt                     <  2:2154277/140‑1 (MQ=255)

                                                                                                                      aaaCGGTGAATATACATTTGATGAATCGAAAGCAATAAAACTAGGGCTTGAGAAAGAT‑GTAATTGATAACGTTAAGAACCTAGAATCTAATGTCTTAGAGCAATTCTACAATCTTAAAGATAGTGAAATTGAAACAGGCt                     <  2:4517647/140‑1 (MQ=255)

                                                                                                                         cGGTGAATATTCATTTGATGAATCGAAAGCAATAAAACTAGGGCTTGAGAAAGAT‑GTAATTGATAACGTTAAGAACCTAGAATCTAATGTCTTAGAGCAATTCTACAATCTTAAAGATATTGAAATTGAAACAGGCTCag                  >  2:3611687/1‑140 (MQ=255)

                                                                                                                         cGGTGAATATTCATTTGATGAATCGAAAGCAATAAAACTAGGGCTTGAGAAAGAT‑GTAATTGATAACGTTAAGAACCTAGAATCTAATGTCTTAGAGCAATTCTACAATCTTAAAGATAGTGAAATTGAAACAGGCTCag                  >  2:3152998/1‑140 (MQ=255)

                                                                                                                                     aTTTGATGAATCGAAAGCAATAAAACTAGGGCTTGAGAAAGAT‑GTAATTGATAACGTTAAGAAACTAGAATCTAATGTCTTAGAGCAATTCTACAATCTTAAAGATAGTGAAATTGAAACAGGCTCAGAGCAATTACAAg      <  2:3312219/140‑1 (MQ=255)

                                                                                                                                     aTTTGATGAATCGAAAGCAATAAAACGAGGGCTTGAGACAGAT‑GTAATTGATAACGTTAAGAACCTAGAATCTAATGTCTTAGAGCAATTCTACAATCTTAAAGATAGTGAAATTGAAACAGGCTCAGAGCAATTACAAg      <  2:3257372/140‑1 (MQ=255)

                                                                                                                                      tttGATGAATCGAAAGCAATAAAACTAGGGCTTGAGAAAGAT‑GTAATTGATAACGTTAAGAACCTAGAATCTAATGTCTTAGAGCAATTCTACAATCTTAAAGATAGTGAAATTGAAACAGGCTCAGAGCAATTACAAgg     <  2:637789/140‑1 (MQ=255)

CTTCTGCAAAAGAAGTAAACCCGAATGTAAATATCTTTCTCAATTCAGCTGTAGAAAATTTGAACCCTGATGACGTTAAACAAACCCTAAAGACAATTAAAGAGTCGATTATCTTAAAAAACGGTGAATATTCATTTGATGAATCGAAAGCAATAAAACTAGGGCTTGAGAAAGAT‑GTAATTGATAACGTTAAGAACCTAGAATCTAATGTCTTAGAGCAATTCTACAATCTTAAAGATAGTGAAATTGAAACAGGCTCAGAGCAATTACAAGGAGA  >  NZ_CP034943/1968024‑1968300

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`