BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	2,350,852	G→T	5.7%	pseudogene (929/1182 nt)	EO946_RS12020 ←	IS3 family transposase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	2,350,852	0	G	T	5.7%	557.4 / 3.3	197	pseudogene (929/1182 nt)	EO946_RS12020	IS3 family transposase
Reads supporting (aligned to +/- strand): ref base G (21/158); new base T (16/2); total (37/160)
Fisher's exact test for biased strand distribution p-value = 1.19e-11
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 201 total aligned reads displayed.

TTCGGTTTGACACGTTGAAAAATATGCATTCTCTGTTTTGAGATGCGAGAAAAACCATTCGATACAGGCATTATCTAGGCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGATATGCTTTTG‑TTGTGTATTGGAAGCCTTGGTCAGAATGGAGGATAGCTCCTTGGACGT‑CCCGTTGGGCTGTCATACTTTCCACTGTCTTCATGACCAGTTCAAGATCATTTCGTTTTGACAGCTTCCATGCGACAATCTCATT  >  NZ_CP034943/2350713‑2350991

ttCGGTTTGACACGTTGAAAAATATGCATTCTCTGTTTTGAGATGCGAGAAAAACCATTCGATACAGGCATTATCTAGGCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGATATg                                                                                                                                               <  2:3786858/140‑1 (MQ=255)

      ttGACACTTTGAAAAATATGCATTATCTGTTTTGAGATGCGAGAAAAACCATTCGATACAGGCATTATCTAGGCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGATATGCTTttg                                                                                                                                         <  1:2748041/140‑1 (MQ=255)

      ttGACACGTTGAAAAATATGCATTCTCTGTTTTGAGATGCGAGAAAAACCATTCGATACAGGCATTATCTAGGCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGATATGCTTttg                                                                                                                                         <  1:4364480/140‑1 (MQ=255)

      ttGACACGTTGAAAAATATGCATTCTCTGTTTTGAGATGCGAGAAAAACCATTCGATACAGGCATTATCTAGGCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGATATGCTTttg                                                                                                                                         <  2:694304/140‑1 (MQ=255)

       tGACACGTTGAAAAATATGCATTCTCTGTTTTGAGATGCGAGAAAAACCATTCGATACAGGCATTATCTAGGCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGTTGTGGTTttg‑t                                                                                                                                       >  1:2884170/1‑140 (MQ=255)

       tGACACGTTGAAAAATATGCATTCTCTGTTTTGAGATGCGAGAAAAACCATTCGATACAGGCATTATCTAGGCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCGTTTTATATTCtttttt                                                                                                                                        >  1:6419/1‑138 (MQ=255)

       tGACACGTTGAAAAATATGCATTCTCTGGTTTGAGATGCGCGAAAAACCATTCGATACAGGCATTATCTAGGCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGGTGTTTGTATGGtttttt                                                                                                                                        >  2:3860489/1‑138 (MQ=255)

               tGAAAAATATGCATTCTCTGTTTTGAGATGCGAGAAAAACCATTCGATACAGGCATTATCTAGGCAGTTTGCCTTACGGGAATGGCTGCGTTTCAAACCAACGGTTTCGAGTCGCTTTTGATAGGGTGTTG‑GTGGGTAtt                                                                                                                               >  2:1119996/1‑140 (MQ=255)

                 aaaaaTATGCATTCTCTGTTTTGAGATGCGAGAAAAACCATTCGATACAGGCATTATCTAGGCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGATATGCTTTTG‑TTGTGTATTgg                                                                                                                             <  2:1228668/140‑1 (MQ=255)

                      tatGCATTCTCTGTTTTGAGATGCGAGAAAAACCATTCGATACAGGCATTATCTAGGCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGATATGCTTTTG‑TTGTGTATTGGAAGcc                                                                                                                        <  2:1623263/140‑1 (MQ=255)

                      tatGCATTCTCTGTTTTGAGATGCGAGAAAAACCATTCGATACAGGCATTATCTAGGCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGATATGCTTTTG‑TTGTGTATTGGAAGcc                                                                                                                        <  2:436360/140‑1 (MQ=255)

                      tatGCATTCTCTGTTTTGAGATGCGAGAAAAACCATTCGATACAGGCATTATCTAGGCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGATATGCTTTTG‑TTGTGTATTGGAAGcc                                                                                                                        <  2:2604344/140‑1 (MQ=255)

                      tatGCATTCTCTGTTTTGAGATGCGAGAAAAACCATTCGATACAGGCATTATCTAGGCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGATATGCTTTTG‑TTGTGTATTGGAAGcc                                                                                                                        <  2:2836652/140‑1 (MQ=255)

                           aTTCTCTGTTTTGAGATGCGAGAAAAACCATTCGATACAGGCATTATCTAGGCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGATATGCTTTTG‑TTGTGTATTGGAAGCCTTGGt                                                                                                                   <  2:786225/140‑1 (MQ=255)

                           aTTCTCTGTTTTGAGATGCGAGAAAAACCATTCGATACAGGCATTATCTAGGCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGATATGCTTTTG‑TTGTGTATTGGAAGCCTTGGt                                                                                                                   <  2:1285972/140‑1 (MQ=255)

                           aTTCTCTGTTTTGAGATGCGAGAAAAACCATTCGATACAGGCATTATCTAGGCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGATATGCTTTTG‑TTGTGTATTGGAAGCCTTGGt                                                                                                                   <  2:259353/140‑1 (MQ=255)

                                ctGTTTTGAGATGCGAGAAAAACCATTCGATACAGGCATTATCTAGGCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGATATGCTTTTG‑TTGTGTATTGGAAGCCTTGGTCAGaa                                                                                                              <  2:1811921/140‑1 (MQ=255)

                                ctGTTTTGAGATGCGAGAAAAACCATTCGATACAGGCATTATCTAGGCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCGTTTGATATTGTTTTG‑TTGTGTATTTGAAGCCTTGGTGAGaa                                                                                                              >  2:4641545/1‑140 (MQ=255)

                                  gTTTTGAGATGCGAGAAAAACCATTCGATACAGGCATTATCTAGGCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGATATGCTTTTG‑TTGTGTATTGGAAGCCTTGGTCAGAATg                                                                                                            <  1:4476992/140‑1 (MQ=255)

                                  gTTTTGAGATGCGAGAAAAACCATTCGATACAGGCATTATCTAGGCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGATATGCTTTTG‑TTGTGTATTGGAAGCCTTGGTCAGAATg                                                                                                            <  2:158318/140‑1 (MQ=255)

                                       gagaTGCGAGAAAAACCATTCGATACAGGCATTATCTAGGCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGATATGCTTTTG‑TTGTGTATTGGAAGCCTTGGTCAGAATggagga                                                                                                       <  2:1376118/140‑1 (MQ=255)

                                         gaTGCGAGAAAAACCATTCGATACAGGCATTATCTAGGCAGTTTGCCTTACGGGCATGGCTGCCTTTCAAACCAACGGGTTCGAGTCGCTTTTGATATGCTTTTG‑TGGTGTATTGGAAGCCTTGGTCAGAATGGAGGATa                                                                                                     <  1:3841611/140‑1 (MQ=255)

                                         gaTGCGAGAAAAACCATTCGATACAGGCATTATCTAGGCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGATATGCTTTTG‑TTGTGTATTGGAAGCCTTGGTCAGAATGGAGGATa                                                                                                     <  1:1476629/140‑1 (MQ=255)

                                         gaTGCGAGAAAAACCATTCGAGACAGGCATTATCTAGGCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGATATGCTTTTG‑TTGTGTATTGGAAGCCTTGGTCAGAATGGAGGATa                                                                                                     <  1:4570554/140‑1 (MQ=255)

                                          aTGCGAGAAAAACCATTCGATACAGGCATTATCTAGGCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGATATGCTTTTG‑TTGTGTATTGGAAGCCTTGGTCAGAATGGAGGATAg                                                                                                    <  1:790485/140‑1 (MQ=255)

                                           tGCGAGAAAAACCATTCGATACAGGCATTATCTAGGCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTTTTGTGCGTTTG‑TTGTTTTTTTGGAGGCGTGGGGACAATGGGGGATAGc                                                                                                   >  1:825474/1‑140 (MQ=255)

                                                  aaaaCCATTCGATACAGGCATTATCTAGGCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGATATGCTTTTG‑TTGTGTATTGGAAGCCTTGGTCAGAATGGAGGATAGCTCCTTgg                                                                                            <  2:325615/140‑1 (MQ=255)

                                                  aaaaCCATTCGATACAGGCATTATCTAGGCAGTGTGCCTTACGGGAATGGCTGCCAGTCAAACCAACGGTTTCGAGTCGCTTTTGATATGCTTTTG‑TTGTGTATTGGAAGCCTTGGTCAGAATGGAGGATAGCTCCTTgg                                                                                            <  2:525455/140‑1 (MQ=255)

                                                     aCCATTCGATACAGGCATTATCTAGGCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGATATGCTTTTG‑TTGTGTATTGGAAGCCTTGGTCAGAATGGAGGATAGCTCCTTGGACg                                                                                         <  1:4730179/140‑1 (MQ=255)

                                                       cATTCGATACAGGCATTATCTAGGCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGATATGCTTTTG‑TTGTGTATTGGAAGCCTTGGTCAGAATGGAGGATAGCTCCTTGGACGT‑c                                                                                      <  1:4915835/140‑1 (MQ=255)

                                                          tCGATACAGGCATTATCTAGGCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCGTTTGATATTCGTTTG‑TTTTTTATTTGAAGCCTTGGTCAGAATGGTGGTTAGCGCCTTGGACGT‑CCCg                                                                                   >  2:4522507/1‑140 (MQ=255)

                                                             aTACAGGCATTATCTAGGCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGATATTCTTTTG‑TTGTGTATTGGAAGCCTTGGTCAGAATGGAGGATAGCTCCTTGGACGT‑CCCGTTg                                                                                <  2:3600188/140‑1 (MQ=255)

                                                             aTACAGGCATTATCTAGGCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGATATGCTTTTG‑TTGTGTATTGGAAGCCTTGGTCAGAATGGAGGATAGCTCCTTGGACGT‑CCCGTTg                                                                                <  2:3842004/140‑1 (MQ=255)

                                                             aTACAGGCATTATCTAGGCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGATATGCTTTTG‑TTGTGTATTGGAAGCCTTGGTCAGAATGGAGGATAGCTCCTTGGACGT‑CCCGTTg                                                                                <  1:4674440/140‑1 (MQ=255)

                                                             aTACAGGCATTATCTAGGCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGATATGCTTTTG‑TTGTGTATTGGAAGCCTTGGTCAGAATGGAGGATAGCTCCTTGGACGT‑CCCGTTg                                                                                <  1:2784945/140‑1 (MQ=255)

                                                               aCAGGCATTATCTAGGCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGATATGCTTTTG‑TTGTGTATTGGAAGCCTTGGTCAGAATGGAGGATAGCTCCTTGGACGT‑CCCGTTggg                                                                              <  2:1959287/140‑1 (MQ=255)

                                                               aCAGGCATTATCTAGGCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGATATGCTTTTG‑TTGTGTATTGGAAGCCTTGGTCAGAATGGAGGATAGCTCCTTGGACGT‑CCCGTTggg                                                                              <  1:2100234/140‑1 (MQ=255)

                                                               aCAGGCATTATCGAGGCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGATATGCTTTTG‑TTGTGTATTGGAAGCCTTGGTCAGAATGGAGGATAGCTCCTTGGACGT‑CCCGTTggg                                                                              <  2:724826/140‑1 (MQ=255)

                                                               aCAGGCATTATCGAGGCAGTTTGCCTTACGGGAATGGCTGCCATTCAAACCAACGGTTTCGAGTCGCTTTTGATATGCTTTTG‑TTGTGTATTGGAAGCCTTGGTCAGAATGGAGGATAGCTCCTTGGACGT‑CCCGTTggg                                                                              <  2:1522665/140‑1 (MQ=255)

                                                                    cATTATCTAGGCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGATATGCTTTTG‑TTGTGTATTGGAAGCCTTGGTCAGAATGGAGGATAGCTCCTTGGACGT‑CCCGTTGGGCTGTc                                                                         <  2:4210234/140‑1 (MQ=255)

                                                                    cATTATCTAGGCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGATATGCTTTTG‑TTGTGTATTGGAAGCCTTGGTCAGAATGGAGGATAGCTCCTTGGACGT‑CCCGTTGGGCTGTc                                                                         <  1:1215208/140‑1 (MQ=255)

                                                                    cATTATCTAGGCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGATATGCTTTTG‑TTGTGTATTGGAAGCCTTGGTCAGAATGGAGGATAGCTCCTTGGACGT‑CCCGTTGGGCTGTc                                                                         <  1:547491/140‑1 (MQ=255)

                                                                    cATTATCTAGGCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGATATGCTTTTG‑TTGTGTATTGGAAGCCTTGGTCAGAATGGAGGATAGCTCCTTGGACGT‑CCCGTTGGGCTGTc                                                                         <  1:4209240/140‑1 (MQ=255)

                                                                    cATTATCTAGGCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGATATGCTTTTG‑TTGTGTATTGGAAGCCTTGGTCAGAATGGAGGATAGCTCCTTGGACGT‑CCCGTTGGGCTGTc                                                                         <  1:303144/140‑1 (MQ=255)

                                                                    cATTATCTAGGCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGATATGCTTTTG‑TTGTGTATTGGAAGCCTTGGTCAGAATGGAGGATAGCTCCTTGGACGT‑CCCGTTGGGCTGTc                                                                         <  1:1688358/140‑1 (MQ=255)

                                                                      ttATCTAGGCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGATATGCTTTTG‑TTGTGTATTGGAAGCCTTGGTCAGAATGGAGGATAGCTCCTTGGACGT‑CCCGTTGGGCTGTCAt                                                                       <  1:1380989/140‑1 (MQ=255)

                                                                      ttATCTAGGCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGATATGCTTTTG‑TTGTGTATTGGAAGCCTTGGTCAGAATGGAGGATAGCTCCTTGGACGT‑CCCGTTGGGCTGTCAt                                                                       <  1:2350879/140‑1 (MQ=255)

                                                                      ttATCTAGGCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGATATGCTTTTG‑TTGTGTATTGGAAGCCTTGGTCAGAATGGAGGATAGCTCCTTGGACGT‑CCCGTTGGGCTGTCAt                                                                       <  1:4628672/140‑1 (MQ=255)

                                                                      ttATCTAGGCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGATATGCTTTTG‑TTGTGTATTGGAAGCCTTGGTCAGAATGGAGGATAGCTCCTTGGACGT‑CCCGTTGGGCTGTCAt                                                                       <  1:223160/140‑1 (MQ=255)

                                                                      ttATCTAGGCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGATATGCTTTTG‑TTGTGTATTGGAAGCCTTGGTCAGAATGGAGGATAGCTCCTTGGACGT‑CCCGTTGGGCTGTCAt                                                                       <  1:1933163/140‑1 (MQ=255)

                                                                      ttATCTAGGCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGATATGCTTTTG‑TTGTGGATTGGAAGCCTTGGTCAGAATGGAGGATAGCTCCTTGGACGT‑CCCGTTGGGCTGTCAt                                                                       <  1:1861927/140‑1 (MQ=255)

                                                                        aTCTAGGCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGATATGCTTTTG‑TTGTGTATTGGAAGCCTTGGTCAGAATGGAGGATAGCTCCTTGGACGT‑CCCGTTGGGCTGTCATAc                                                                     <  2:3168835/140‑1 (MQ=255)

                                                                        aTCTAGGCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGATATGCTTTTG‑TTGTGTATTGGAAGCCTTGGTCAGAATGGAGGATAGCTCCTTGGACGT‑CCCGTTGGGCTGTCATAc                                                                     <  1:3345525/140‑1 (MQ=255)

                                                                        aTCTAGGCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGATATGCTTTTG‑TTGTGTATTGGAAGCCTTGGTCAGAATGGAGGATAGCTCCTTGGACGT‑CCCGTTGGGCTGTCATAc                                                                     <  1:2028620/140‑1 (MQ=255)

                                                                        aTCTAGGCAGTTTGCCGTACGGGACTGGCTGCCTTTCAAACCAACGGTTTCGTTTCGCGGTTGATATGGTTTTG‑GTTTGTTTTGGAAGCCGTGGTACCACTGGAGGATATCTGCATTGAAGT‑CCCCGTGGGCGGTCATaa                                                                     >  2:4016422/1‑139 (MQ=255)

                                                                             ggCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGTGATTGGTTTT‑TTTTGTATTGGTAGCCGTGGGGAGAATGGGGGGTATCTCCGTGGGCGT‑CCCGGTGGGGTGTCATAAtttga                                                                >  1:3949803/1‑138 (MQ=255)

                                                                             ggCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGATATGCTTTTG‑TTGTGTATTgg                                                                                                                             <  1:2801094/80‑1 (MQ=255)

                                                                             ggCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCGTTTGTTTTTGTTTTG‑TTGTTTTTTGGTATGCTTGGTGAGAATTGTGGGTAGCTCCTTGGAGGT‑CCCGGTTGGGTGTGATACtttac                                                                >  1:2319395/1‑138 (MQ=255)

                                                                             ggCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCGTTTGTTATTCGTTTT‑TTGTGTTTTTTTAGCCTTGGGGAGAATTTGGGTTAGGGCCTTGGACGT‑ACCGTTGGGGTGTGATACtttac                                                                >  1:4894862/1‑138 (MQ=255)

                                                                             ggCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCGTTTGTTATGCGTTTG‑TTGTGTTTTTGTATCCATTGTGAGAATTGAGGGTATCGCCGTGGTCGT‑CCCCTTGGGGTGTCATACatttc                                                                >  1:4802350/1‑138 (MQ=255)

                                                                             ggCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCGTGTGTTATGGTTTTGTTTTTGTTTTGG‑AGCCATGGTCCGAATTGGGGCTAGGTCCATGGACGTGCCCGTTGGG‑GGTGATACtttac                                                                >  1:2144637/1‑138 (MQ=255)

                                                                              gCAGTTTGCCTTACGGGTATGGCTGCCTTTCAAACCTACGGTTTCGAGTCGCTTTTTATATGCTTTTG‑TTTTGTATTGGAAGCCTTGGTCAGAATGGAGGATAGCTCCTTGGACGT‑CCCGTTGGGCTGTCATACTTTCCa                                                               <  2:3712308/140‑1 (MQ=255)

                                                                              gCAGTTTGCCTTACGGGCATGGCTGCCTTTCAAACCAACGGTTTCTAGTCGCTTTTGATATGCTTTTG‑TTGTGTATTGGAAGCCTTGGTCAGAATGGAGGATAGCTCCTTGGACGT‑CCCGTTGGGCTGTCATACTTTCCa                                                               <  2:3774253/140‑1 (MQ=255)

                                                                              gCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGATATGCTTTTG‑TTGTGTATTGGAAGCCTTGGTCAGAATGGAGGATAGCTCCTTGGACGT‑CCCGTTGGGCTGTCATACTTTCCa                                                               <  2:671533/140‑1 (MQ=255)

                                                                              gCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGATATGCTTTTG‑TTGTGTATTGGAAGCCTTGGTCAGAATGGAGGATAGCTCCTTGGACGT‑CCCGTTGGGCTGTCATACTTTCCa                                                               <  2:3188163/140‑1 (MQ=255)

                                                                                aGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGATATGCTTTTG‑TTGTGTATTGGAAGCCTTGGTCAGAATGGAGGATAGCTCCTTGGACGT‑CCCGTTGGGCTGTCATACTTTCCACt                                                             <  2:2180143/140‑1 (MQ=255)

                                                                                aGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGATATGCTTTTG‑TTGTGTATTGGAAGCCTTGGTCAGAATGGAGGATAGCTCCTTGGACGT‑CCCGTTGGGCTGTCATACTTTCCACt                                                             <  1:3024275/140‑1 (MQ=255)

                                                                                aGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGATATGCTTTTG‑TTGTGTATTGGAAGCCTTGGTCAGAATGGAGGATAGCTCCTTGGACGT‑CCCGTTGGGCTGTCATACTTTCCACt                                                             <  2:2976166/140‑1 (MQ=255)

                                                                                aGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGATATGCTTTTG‑TTGTGTATTGGAAGCCTTGGTCAGAATGGAGGATAGCTCCTTGGACGT‑CCCGTTGGGCTGTCATACTTTCCACt                                                             <  2:3080722/140‑1 (MQ=255)

                                                                                aGTTTGCCTTACGGGAATGGCGGCCTTGCAAACCAACGGTTTCGAGTCGCTTTTGATATGCTTTTG‑TTGTGTATTGGAAGCCTTGGTCAGAATGGAGGATAGCTCCTTGGACGT‑CCCGTTGGGCTGTCATACTTTCCACt                                                             <  2:4167407/140‑1 (MQ=255)

                                                                                  tttGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTTATATGCTGTTT‑TTTTGTATTGGAAGCCTTTGTCACAATGTATGAAATCTCCTTGGACTG‑CCCCATTGTCTGTCAAAAATTCCACAGt                                                           >  2:3470371/1‑140 (MQ=255)

                                                                                  tttGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCGTTTTGTATTGTTTTG‑TTTTGTTTTTTGAGCCGTTGTGAGCATTGGGGGTTGCTCCGTGGGCGT‑ACCGGTGGGGTGTCGTACTTTcaccagt                                                           >  1:522517/1‑134 (MQ=255)

                                                                                  tttGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCGTTTGTTTTTGTTTTT‑TTGTGTATTGGTAGCCGTTGTGAGAATTGGGGGTAGCTCCGTGGACGT‑CCCGGTGGGGGGTGATACtttgccatgt                                                           >  1:3983612/1‑133 (MQ=255)

                                                                                  tttGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCGTTTGATATGCTTTTG‑TTGTGTATTTGAAGCCTTTGTCAGAATGGAGGATAGCTCCTTGGACGT‑CCCGTTGGGCTGTCATACTTTCCACTGt                                                           >  2:4981653/1‑140 (MQ=255)

                                                                                  tttGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCGTTTGAAATGCTGTTG‑GTGTGGTTTGGAACCCGTGGGGAGAATGGAGGATAGCTGCGTGGACGT‑CCCGGTGGGCTGTCATACTTTCCACGGt                                                           >  2:4606577/1‑140 (MQ=255)

                                                                                       cTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGATATGCTTTTT‑TTGTGTATTGGAAGCCTTGGTCAGAATGGAGGATAGCTCCTTGGACGT‑CCCGTTGGGCTGTCATACTTTCCACTGTCTTCa                                                      <  2:2736548/140‑1 (MQ=255)

                                                                                       cTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGATATGCTTTTG‑TTGTGTATTGGAAGCCTTGGTCAGAATGGAGGATAGCTCCTTGGACGT‑CCCGTTGGGCTGTCATACTTTCCACTGTCTTCa                                                      <  2:809031/140‑1 (MQ=255)

                                                                                       cTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGATATGCTTTTG‑TTGTGTATTGGAAGCCTTGGTCAGAATGGAGGATAGCTCCTTGGACGT‑CCCGTTGGGCTGTCATACTTTCCACTGTCTTCa                                                      <  2:1690425/140‑1 (MQ=255)

                                                                                           cGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGATATGCTTTTG‑TTGTGTATTGGAAGCCTTGGTCAGAATGGAGGATAGCTCCTTGGACGT‑CCCGTTGGGCTGTCATACTTTCCACTGTCTTCATGAc                                                  <  2:337858/140‑1 (MQ=255)

                                                                                           cGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGATATGCTTTTG‑TTGTGTATTGGAAGCCTTGGTCAGAATGGAGGATAGCTCCTTGGACGT‑CCCGTTGGGCTGTCATACTTTCCACTGTCTTCATGAc                                                  <  2:551676/140‑1 (MQ=255)

                                                                                           cGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGATATGCTTTTG‑TTGTGTATTGGAAGCCTTGGTCAGAATGGAGGATAGCTCCTTGGACGT‑CCCGTTGGGCTGTCATACTTTCCACTGTCTTCATGAc                                                  <  1:3063976/140‑1 (MQ=255)

                                                                                           cGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGATATGCTTTTG‑TTGTGTATTGGAAGCCTTGGTCAGAATGGAGGATAGCTCCTTGGACGT‑CCCGTTGGGCTGTCATACTTTCCACTGTCTTCATGAc                                                  <  1:3590142/140‑1 (MQ=255)

                                                                                           cGGGAAGGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTGTGATATGCTTTTG‑TTGTGTATTGGAAGCCTTGGTCAGAATGGAGGATAGCTCCTTGGACGT‑CCCGTTGGGCTGTCATACTTTCCACTGTCTTCATGAc                                                  <  2:577028/140‑1 (MQ=255)

                                                                                                aTGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGATATGCTTTTG‑TTGTGTATTGGAAGCCTTGGTCAGAATGGAGGATAGCTCCTTGGACGT‑CCCGTTGGGCTGTCATACTTTCCACTGTCTTCATGACCAGtt                                             <  1:928070/140‑1 (MQ=255)

                                                                                                  ggCTGCCTTTCACACCAACGGTTTCGAGTCGCTTTTGATATGCTTTTG‑TTGTGTATTGGAAGCCTTGGTCAGAATGGAGGATAGCTCCTTGGACGT‑CCCGTTGGGCTGTCATACTTTCCACTGTCTTCATGACCAGTTCa                                           <  2:4388923/140‑1 (MQ=255)

                                                                                                                      gTTTCGATTCGCTTTTGATATGCTTTTG‑TTGTGTATTGGAAGCCTTGGTCAGAATGGAGGATAGCTCCTTGGACGT‑CCCGTTGGGCTGTCATACTTTCCACGGTCTTCATGACCAGTTCAAGATCATTTCGTTTTGACAg                       <  2:1847842/140‑1 (MQ=255)

                                                                                                                      gTTTCGAGTCGCTTTTGATATGCTTTTG‑TTGTGTATTGGAAGCCTTGGTCAGAATGGAGGATAGCTCCTTGGACGT‑CCCGTTGGGCTGTCATACTTTCCACTGTCTTCATGACCAGTTCAAGATCATTTCGTTTTGACAg                       <  2:2772758/140‑1 (MQ=255)

                                                                                                                                 cTTTTGATATGCTTTTG‑TTGTGTATTGGAAGCCTTGGTCAGAATGGAGGATAGCTCCTTGGACGT‑CCCGTTGGGCTGTCATACTTTCCACTGTCTTCATGACCAGTTCAAGATCATTTCGTTTTGACAGCTTCCATGCGa            <  1:2599846/140‑1 (MQ=255)

                                                                                                                                  ttttGATATGCTTTTG‑TTGTGTATTGGAAGCCTTGGTCAGAATGGAGGATAGCTCCTTGGACGT‑CCCGTTGGGCTGTCATACTTTCCACTGTCTTCATGACCAGTTCAAGATCATTTCGTTTTGACAGCTTCCATGCGAc           <  1:2442434/140‑1 (MQ=255)

                                                                                                                                     tgttaagCTTTTG‑TTGTGTATTTGAACCCTTGGTCAGAATGGAGGATAGCTCCGTGGACGT‑CCCGTTGGGCTGTCATAATTTCCACTGTGTTCAAGACCAGTTCAATATCATTTGGTTTTTTCAGCTTACAATCGACaaa        >  2:2299046/7‑139 (MQ=255)

                                                                                                                                     tGATATGCTTTTG‑TTGTGTATTGGAAGCCTTGGTCAGAATTGAGGATAGCTCCTTGGACGT‑CCCGTTGGGCTGTCATAATTTCCACTGTCTTCATTACCAGTTAAAGATCATTTCGTTTTTAAAGCTTCCATTCGACAAt        >  2:4872197/1‑140 (MQ=255)

                                                                                                                                         atGCTTTTG‑TTGTGTATTGGAAGCCTTTGTCAGAATGGAGGATAGCTCCTTGGACGT‑CCCGTTGGGCTGTCATACTTTCCACTGTCTTCATGACCAGTTCAAGATCATTTCGTTTTGACAGCTTCCATGAGACAATCTCa    >  2:2490918/1‑140 (MQ=255)

                                                                                                                                         atGCTTTTG‑TTGTGTATTGGAAGCCTTGGTCAGAATGGAGGATAGCTCCTTGGACGT‑CCCGTTGGGCTGTCATACTTTCCACTGTCTTCATGACCAGTTCACGATCATTTCGTTTTGACAGCTTCCATGCGACAATCTCa    <  2:1596383/140‑1 (MQ=255)

                                                                                                                                         atGCTTTTG‑TTGTGTATTGGAAGCCTTGGTCAGAATGGAGGATAGCTCCTTGGACGT‑CCCGTTGGGCTGTCATACTTTCCACTGTCTTCATGACCAGTTCAAGATCATTTCGTTTTGACAGCTTCCATGCGACAATCTCa    <  1:3326093/140‑1 (MQ=255)

                                                                                                                                         atGCTTTTG‑TTGTGTATTGGAAGCCTTGGTCAGAATGGAGGATAGCTCCTTGGACGT‑CCCGTTGGGCTGTCATACTTTCCACTGTCTTCATGACCAGTTCAAGATCATTTCGTTTTGACAGCTTCCATGCGACAATCTCa    <  1:4861003/140‑1 (MQ=255)

                                                                                                                                         atGCTTTTG‑TTGTGTATTGGAAGCCTTGGTCAGAATGGAGGATAGCTCCTTGGACGT‑CCCGTTGGGCTGTCATACTTTCCACTGTCTTCATGACCAGTTCAAGATCATTTCGTTTTGACAGCTTCCATGCGACAATCTCa    <  1:103526/140‑1 (MQ=255)

                                                                                                                                         atGCTTTTG‑TTGTGTATTGGAAGCCTTGGTCAGAATGGAGGATAGCTCCTTGGACGT‑CCCGTTGGGCGGTCATACTTTCCACTGTCTTCATGACCAGTTCAAGATCATTTCGTTTTGACAGCTTCCATGCGACAATCTCa    <  1:4028370/140‑1 (MQ=255)

                                                                                                                                         atGCTTTTG‑TTGTGTATTGGAAGCCTTGGTCAGAATGGAGGATAGCTCCTTGGACGT‑CCCGGTGGGCTGTCATACTTTCCACTGTCTTCATGACCAGTTCAAGATCATTTCGTTTTGACAGCTTCCATGCGACAATCTCa    <  1:3267655/140‑1 (MQ=255)

                                                                                                                                         atGCTTTTG‑GTGTGTATTGGAAGCCTTGGTCAGAATGGAGGATAGCTCCTTGGACGT‑CCCGTTGGGCTGTCATACTTTCCACTGTCTTCATGACCAGTTCAAGATCATTTCGTTTTGACAGCTTCCATGCGACAATCTCa    <  1:2857402/140‑1 (MQ=255)

                                                                                                                                           gCTTTTG‑TTGTGTATTGGAAGCCTTGGTCAGAATGGAGGATAGCTCCTTGGACGT‑CCCGTTGGGCTGTCATACTTTCCACTGTCTTCATGACCAGTTCAAGATCATTTCGTTTTGACAGCTTCCATGCGACAATCTCAtt  >  1:1262251/1‑140 (MQ=255)

TTCGGTTTGACACGTTGAAAAATATGCATTCTCTGTTTTGAGATGCGAGAAAAACCATTCGATACAGGCATTATCTAGGCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGATATGCTTTTG‑TTGTGTATTGGAAGCCTTGGTCAGAATGGAGGATAGCTCCTTGGACGT‑CCCGTTGGGCTGTCATACTTTCCACTGTCTTCATGACCAGTTCAAGATCATTTCGTTTTGACAGCTTCCATGCGACAATCTCATT  >  NZ_CP034943/2350713‑2350991

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`