BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	2,991,245	G→T	18.0%	intergenic (+46/‑161)	gbsR → / → bslA	transcriptional regulator GbsR/biofilm‑surface layer protein BslA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	2,991,245	0	G	T	18.0%	314.6 / 38.1	177	intergenic (+46/‑161)	gbsR/bslA	transcriptional regulator GbsR/biofilm‑surface layer protein BslA
Reads supporting (aligned to +/- strand): ref base G (50/91); new base T (33/2); total (83/94)
Fisher's exact test for biased strand distribution p-value = 7.01e-11
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 180 total aligned reads displayed.

CTCTAAAGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAA‑ACGCCTGGGAAACCGGGCGT‑TTTTTTGTT‑C‑TTTTTTATGTAATTAC‑T‑AC‑AAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCGGGCAGGCCTGAATATTTTTATAA  >  NZ_CP034943/2991109‑2991384

ctctAAAGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAA‑ACGCCTGGGAAACCGGGCTT‑TTTTTTTTT‑G‑TTTTTTATTTaa                                                                                                                                             >  2:772234/1‑140 (MQ=255)

ctctAAAGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAA‑ACGCCTGGGAAACCGGGCGT‑TTTTTTGTT‑C‑TTTTTTATGTaa                                                                                                                                             >  2:4001350/1‑140 (MQ=255)

ctctAAAGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAA‑ACGCCTGGGAAACCGGGCGT‑GTTTTTGTT‑A‑TTTTTTTTGTaa                                                                                                                                             >  2:211437/1‑140 (MQ=255)

ctctAAAGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAA‑ACGCCTGGGAAACCGGGCGT‑GGTTTTGTT‑A‑TTTTTTATGTaa                                                                                                                                             >  2:4187722/1‑140 (MQ=255)

 tctAAAGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAACACAAAC‑ACGCCTGGGAAACCGGGCGT‑TTTTTTGTT‑C‑TTTTTTATGTAAt                                                                                                                                            <  2:4931872/140‑1 (MQ=255)

     aaGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAA‑ACGCCTGGGAAACCGGGCGT‑TTTTTTTTT‑G‑TTTTTTTTGttattaat                                                                                                                                        >  2:2461420/1‑138 (MQ=255)

        cTTACTTTTATTGGTTGAAACGGCTTGCTTAATCGGTGCAGAGCGGGTAAATTTTTAAATTTATACCGATTTAAACAAAGTAAAACAAAA‑ACGCCTGGGAAACCGGGCGT‑TTTTTTGTT‑C‑TTTTTTATGTAATTAC‑T‑AC‑a                                                                                                                                  <  2:3835830/140‑1 (MQ=255)

        cTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAA‑ACGCCTGGGAAACCGGGCGT‑TTTTTTGTT‑C‑TTTTTTATGTAATTAC‑T‑AC‑a                                                                                                                                  <  2:3547669/140‑1 (MQ=255)

        cTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAA‑ACGCCTGGGAAACCGGGCGT‑TTTTTTGTT‑C‑TTTTTTATGTAATTAC‑T‑AC‑a                                                                                                                                  <  2:2073320/140‑1 (MQ=255)

        cTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAAAAAAA‑ACGCCTGGGAAACCGGGCGT‑TTTTTTGTT‑C‑TTTTTTATGTAATTAC‑T‑AC‑a                                                                                                                                  <  2:4789226/140‑1 (MQ=255)

          tACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATAGCGATTGAAACAAATTAAAACAAAA‑ACTCCTGGGAAACCGGGCGT‑TTTTTTTTT‑G‑TTTTTTTTGTAATTAA‑T‑Aa‑aaa                                                                                                                                >  2:4581832/1‑140 (MQ=255)

          tACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAA‑ACGCCTGGTAAACCGCGCGT‑TTTATTTTT‑C‑TTTTTTATGTAATTAA‑T‑Aa‑aaa                                                                                                                                >  2:4133209/1‑140 (MQ=255)

          tACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAA‑ACGCCTGGGAAACCGGGCGT‑TTTTTTTTT‑G‑TTTTTTATGTAATTAA‑T‑Aa‑aaa                                                                                                                                >  2:2753671/1‑140 (MQ=255)

          tACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAA‑ACGCCTGGGAAACCGGGCGT‑TTTTTTTTT‑A‑TTTTTTATGTAATTAC‑A‑AC‑aaa                                                                                                                                >  2:901683/1‑140 (MQ=255)

          tACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAA‑ACGCCTGGGAAACCGGGCGT‑TTTTTTGTT‑C‑TTTTTTATGTAATTAC‑T‑AC‑aaa                                                                                                                                >  2:2496994/1‑140 (MQ=255)

          tACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAA‑ACGCCTGGGAAACCGGGCGT‑GTTTTTGTT‑A‑TTTTTTTTTTAATTAA‑T‑Aa‑aaa                                                                                                                                >  1:2321722/1‑140 (MQ=255)

          tACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGAAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAATCAAAA‑ACGCCGGGGAAACCGGGCGT‑TTTTTTGTT‑G‑TTTTTTATTTACATAG‑T‑AC‑aaa                                                                                                                                >  2:2006236/1‑140 (MQ=255)

           aatattatTGGCTGAAACGGATTGCTGAATCGGTGCAGAGCGGGTAAATTTTTAAATTTATACCGATTGAAACAAATTAAAACAAAA‑ACGCCTGGGAAACCGGGCGT‑TTTTTTGTT‑C‑TTTTTTATGTAATTAC‑T‑AC‑AAAt                                                                                                                               <  2:691007/138‑1 (MQ=255)

           aCTATGATTGGCTGAAACGGCTTGCTTAATCGTTGCAGAGCGTGTAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAA‑ACGCCTGGGAAACCGGGCGT‑TTTTTTGTT‑C‑TTTTTTATGTAATTAC‑T‑AC‑AAAt                                                                                                                               <  2:3143486/140‑1 (MQ=255)

               tgtttGGCTGAAAGGGCTTGCTTAATCGTTGCAGAGCGGGGAATTTTTTAAATTTATACCGATTGAAAAAAAGTAAAACAAAA‑ACGCCTGGGAAACCGGGCG‑‑TTTTTTGTT‑C‑TTTTTTATGTAATTAC‑T‑AC‑AAATTTGaa                                                                                                                          <  1:3712138/137‑1 (MQ=255)

                 aTTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAA‑ACGCCTGGGAAACCGGGCGT‑TTTTTTTTT‑T‑TTTTTTTTTTAATTAA‑T‑AA‑AAAtttttaa                                                                                                                         >  1:3468458/1‑136 (MQ=255)

                 aTTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAA‑ACGCCTGGGAAACCGGGCGT‑GTTTTTTTT‑C‑TTTTTTTTTTAATTAA‑T‑AA‑AAAattttaa                                                                                                                         >  1:4820925/1‑136 (MQ=255)

                  ttGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAA‑ACGCCTGTGAAACCGTGCGT‑TTTTTTGTT‑G‑TTTTTTATTTTATTAC‑T‑AC‑AAAatttataa                                                                                                                        >  2:366408/1‑135 (MQ=255)

                  ttGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAA‑ACGCCTGGGAAACCGGGCGT‑GGTTTTGTT‑G‑TTTTTTTTGTTATTAA‑T‑AC‑AAAtttttaaa                                                                                                                        >  1:427266/1‑135 (MQ=255)

                    ggCTTAAACGGGTTGCTGAATCGGTGCACAGGGGGGAAATTTTTAAATTTATACCGATTGAAAAAAATTAAAACAAAC‑ACGCCTGGGAAACCGGGCGT‑TTTTTTGTT‑C‑TTTTTTATGTAATTAC‑T‑AC‑AAATTTGAAAAAt                                                                                                                      <  1:2089939/140‑1 (MQ=255)

                    ggCTTAAACGGATTGCTTAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAATTAAAACAAAA‑ACGCCTGGGAAACCGGGCGT‑TTTTTTGTT‑C‑TTTTTTATGTAATTAC‑T‑AC‑AAATTTGAAAAAt                                                                                                                      <  1:4852119/140‑1 (MQ=255)

                    ggCTGAAACGGCTTGCTGAATCGGTGCAGAGGGGTTAAATTTTTAAATTTATACCTATTTAAACAAAGTAAAAAAAAA‑CCGCCTGGGAAACCGGGCGT‑TTTTTTGTT‑C‑TTTTTTATGTAATTAC‑T‑AC‑AAATTTGAAAAAt                                                                                                                      <  1:1500065/140‑1 (MQ=255)

                     gCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAA‑ACGCCTGGGAAACCGGGCGT‑GTTTTTGTT‑G‑TTTTTTATTTTATTTC‑T‑AC‑AAATTTGtaaaaaa                                                                                                                     >  1:3924839/1‑138 (MQ=255)

                     gCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAA‑ACGCCTGGGAAACCGGGCGT‑GTTTTTGTT‑G‑TTTTTTATGTAATTAC‑T‑AC‑AAATTTTAAAAATa                                                                                                                     >  2:3436546/1‑140 (MQ=255)

                     gCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAA‑ACGCCTGGGAAACCGGGCGT‑GTTTTTGTT‑A‑TTTTTTTTGTAATTAA‑T‑AA‑AAAATTTAAAAATa                                                                                                                     >  2:2215579/1‑140 (MQ=255)

                     gCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAA‑ACGCCTGGGAAACCGGGCGT‑GTTTTTGTT‑A‑TTTTTTATTTAATTAC‑T‑AA‑AAATTTCaaaaaaa                                                                                                                     >  2:3124377/1‑138 (MQ=255)

                     gCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAA‑ACGCCTGGGAAACCGGGCGT‑GTGTTTGTT‑G‑TTTTTTTTGTAATTAA‑T‑AA‑AAATTTTTAAAATa                                                                                                                     >  2:1832313/1‑140 (MQ=255)

                       tGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAA‑ACGCCTGGGAAACCGGGCGT‑TGTTTTTTT‑G‑TTTTTTTTTTTATTAA‑TAAA‑AAATTTTaaaaaaag                                                                                                                    >  1:3606234/1‑137 (MQ=255)

                       tGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAA‑ACGCCTGGGAAACCGGGCGT‑GTTTTTGTT‑C‑TTTTTTATGTAATTAA‑T‑AC‑AAAATTTAAAAATaag                                                                                                                   >  2:4690356/1‑138 (MQ=255)

                           aCGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAA‑ACGCCTGGGAAACCGGGCGT‑TTTTTTGTT‑G‑TTTTTTATGTAATTAC‑T‑AC‑AAATTTTAAAAATAAgggca                                                                                                               >  2:4239498/1‑138 (MQ=255)

                               cTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAA‑ACGCCTGGGAAACCGGGCGT‑GGTTTTGTT‑G‑TTTTTTTTGTAATTAA‑T‑AC‑AAATTTGAAAAATAAGGGaaaaaa                                                                                                           >  2:4370844/1‑139 (MQ=255)

                               cTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACAGATTGAAACAAAGTAAAACAAAA‑ACGCCTGGGAAACCGGGCGG‑GTTTTTGTT‑G‑TTTTTTATGTTATTAG‑T‑AC‑AACTGTGAAAAATAAGCGtaaaaa                                                                                                           >  2:2893908/1‑139 (MQ=255)

                                 tGCTGAATCGGTGCAGAGCGGGGAAATTTTTAATTTTATACCGATTGAAACAAAGTAAAACAAAA‑ACGCCTGGGAAACCGGGCGT‑GGTTTTGTT‑G‑TTTTTTTTGTTGTTTCTT‑AC‑AAATTGAAAAAAAAGGGGAAAGAct                                                                                                          >  1:4377655/1‑139 (MQ=255)

                                  gCTTAATCTGTGCATAGCGGGTAAATTTTTAAATTTATACCGTTTGAAAAAAAGTAAAACAAAA‑ACGCCTGGGAAACCGGGCGT‑TTTTTTGTT‑C‑TTTTTTATGTAATTAC‑T‑AC‑AAATTTGAAAAATACGGGAAAAACATc                                                                                                        <  2:1704942/140‑1 (MQ=255)

                                       aTCGGTGCAGAGCGGGGAAAGTTTTCAATTTATACCGATTGAAACAAAGTAAAACAAAC‑ACGCCTGGGAAACCGGGCGT‑TTTTTTGTT‑C‑TTTTTTATGTAATTAC‑T‑AC‑AAATTTGAAAAATACGGGAAAAACATCCCAtt                                                                                                   <  2:4020551/140‑1 (MQ=255)

                                            tGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAA‑ACTCCTGGGAAACCGGGCGT‑GTTTTTTTT‑A‑TTTTTTATTTACTTAA‑T‑AA‑AAATTTTAAAAATAACGGAAAAAAATACCATTTAAtt                                                                                              >  2:3435750/1‑140 (MQ=255)

                                            tGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAA‑ACGCCTGGGAAACCGGGCGT‑GTTTTTGTT‑A‑TTTTTTATGTAATTAC‑T‑AC‑AAATTTTTACAATTAGGGAAAAAGATGCCAttttatt                                                                                              >  2:3435739/1‑136 (MQ=255)

                                               agagCGGGCAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAA‑ACGCCTGGGAAACCGGGCGT‑TTTTTTGTT‑C‑TTTTTTATGTAATTAC‑T‑AC‑AAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCa                                                                                           <  1:3436479/140‑1 (MQ=255)

                                                 agCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAA‑ACGCCTGGGAAACCGGGCGT‑TTTTTTGTT‑G‑TTTTTTATTTTATTAA‑T‑AA‑AAAATTGAAAAATACGGGGAAAACATCACATTTAATTCCaaa                                                                                         >  2:4299963/1‑140 (MQ=255)

                                                   cGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAA‑ACGCCTGGGAAACCGGGCGT‑GTTTTTTTT‑G‑TTTTTTTTTTAATTAA‑TAAA‑AAATTTTAAAATTATGGAAAAAAAAAACAATTT‑CTTACAAAat                                                                                       >  1:3660193/1‑140 (MQ=255)

                                                      ggAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAA‑TCGCCTGGGAAACCGGGCGT‑GTGTTTGTTGG‑TTTTTTTTTTAGTTA‑‑T‑AC‑AAATTTTTAAAAAACGGGAAAAAAAACCCATTTACTTGCAAAatagt                                                                                    >  1:2312248/1‑138 (MQ=255)

                                                      ggAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAA‑ACGCCTGGGAAACCGGGCGTGGTTTTTTTT‑T‑TTTTTTTTTTATTTAA‑T‑CA‑AAATTTTAAAATAATGGAAAAAAAAACACATTTA‑TTACAAAAAAtt                                                                                    >  1:1372827/1‑140 (MQ=255)

                                                      ggAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAA‑ACGCCTGGGAAACCGGGCGT‑TGTTTTGTT‑G‑TTTTTTATGTAATTAA‑T‑AC‑AAATTTGAAAAATACGGGGAAAACATCCCCTTTTAGTCCAAAATAtt                                                                                    >  2:4644800/1‑140 (MQ=255)

                                                      ggAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAA‑ACGCCTGGGAAACCGGGCGT‑GTTTTTTTT‑T‑TTTTTTTTTTAATTAA‑T‑AA‑AAAATTTAAAAAAAAAGGCAAAACATAAAAAATAATTCGAAAATAtt                                                                                    >  1:1986751/1‑140 (MQ=255)

                                                      ggAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAA‑ACGCCTGGGAAACCGGGCGT‑GTTTTTTTT‑G‑TTTTTTTTTTAATATA‑A‑AA‑AAATTTTAAAAATAAAGGCAAAAAAAACCAGATAATTAAAAAATAtt                                                                                    >  1:1715971/1‑140 (MQ=255)

                                                      ggAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAA‑ACGCCTGGGAAACCGGGCGT‑GTTTTTGTT‑T‑TTTTTTTTTTTATTTA‑T‑ACAAAATTTTAAAAAAAGGGGAAAAA‑ATACCCATTACTTACAAAATAtt                                                                                    >  1:1774745/1‑140 (MQ=255)

                                                      ggAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAA‑ACGCCTGGGAAACCGGGCGT‑GTTTTTGTT‑T‑TTTTTTGTTTAATTAA‑TAAA‑AAATTTAAAAAAAACGGGAAAAAAACCCCA‑TTACATGCAAAAAAtt                                                                                    >  1:275484/1‑140 (MQ=255)

                                                      ggAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAA‑ACGCCTGGGAAACCGGGCGT‑GTTTTTGTT‑G‑TTTTTTATGTAAGTAC‑T‑AA‑AAATTTTTAAAATAAGGGAAAAACATCCCATTTTCTTCCaaagaatg                                                                                    >  2:2661306/1‑135 (MQ=255)

                                                       taaaTTTTTAATTTATTACCGTTTGAAAAAAATAAAAAAAAAA‑ACTCCTGGTAAACAGGGCTT‑TTTTTTGTT‑C‑TTTTTTTTTTAATTAA‑G‑AC‑AAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATAttt                                                                                   <  1:706648/139‑1 (MQ=255)

                                                           tttttAAATTTATACCTATTTAAACAAATTAAAACAAAA‑ACGCCTGGGAAACCGGGCGT‑TTTTTTGTT‑C‑TTTTTTATGTAATTAC‑T‑AC‑AAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATg                                                                               <  2:759017/140‑1 (MQ=255)

                                                            ttttAAATTTATACCGATTGAAACAAAGAAAAACAACA‑ACGCCTGGGAAACCGGGCGT‑TTTTTTGTT‑C‑TTTTTTATGTAATTAC‑T‑AC‑AAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATgg                                                                              <  2:3154816/140‑1 (MQ=255)

                                                                 aaTTTATACCGATTGAAACAAAGTAAAACAAAA‑ACGCCTGGGAAACCGGGCGT‑TTTTTTGTT‑C‑TTTTTTATGTAATTAC‑T‑AC‑AAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAAtt                                                                         <  2:3605014/140‑1 (MQ=255)

                                                                    ttATACCGATTGAAACAAAGTAAACCAAAA‑ACGCCTGGGAAACCGGGCGT‑TTTTTTGTT‑C‑TTTTTTATGTAATTAC‑T‑AC‑AAATTTGAAAAATACGGGAAAAACATCCCATTTACGTCCAAAATATTTGATGGTAATTTTa                                                                      <  2:1793273/140‑1 (MQ=255)

                                                                      ataCCGATTGAAACAAAGTAAAACAAAA‑ACGCCTGGGAAACCGGGCGT‑GTTTTTGTT‑A‑TTTTTTATGTAATTAC‑T‑AC‑AAATTTGAAAAATACGGGAAAAACATACCATTTTATTACAAAATATTTGTTGGTAAttttttt                                                                    >  2:3314180/1‑137 (MQ=255)

                                                                           gATTCAAACATCGTAAAACAAAC‑ACGCCTGGGACACCGGGCGT‑TTTTTTGTT‑C‑TTTTTTATGTAATTAC‑T‑AC‑AAATTTGAACAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGt                                                               <  2:3729423/140‑1 (MQ=255)

                                                                                aaaCAAAGTAAAAAAAAC‑ACGCCTGGGAAACCGGGCGT‑TTTTTTGTT‑C‑TTTTTTATGTAATTAC‑T‑AC‑AAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAAt                                                          <  1:4570575/140‑1 (MQ=255)

                                                                                      aGTAAAACAAAA‑ACGCCTGGGAAACCGGGCGT‑TTTTTTTTT‑A‑TTTTTTTTGTAATTAA‑T‑AC‑AAAATTGAAAAAAAAGGGGAAAACATCCCATTTTAGTGCAAAATATTTGATGTTAATTTTATTTTGGTAAAATTTGttt                                                    >  2:4553194/1‑138 (MQ=255)

                                                                                      aGTAAAACAAAA‑ACGCCTGGGAAACCGGGCGT‑TTTTTTGGT‑A‑TTTTTTATGTAATTAA‑A‑AC‑AAATTTGTAAAAAACCGGAAAAACAACCCATATAAATCCAAAAAATTTTATGGTAAATTTATTTAGGTAAAATTTGTAt                                                    >  2:2347976/1‑140 (MQ=255)

                                                                                          aaccAAAA‑ACGCCTGGGAAACCGGGCGT‑TTTTTTGTT‑C‑TTTTTTATGTAATTAC‑T‑AC‑AAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                                                <  2:1295608/137‑1 (MQ=255)

                                                                                          aaccAAAA‑ACGCCTGGGAAACCGGGCGT‑TTTTTTGTT‑C‑TTTTTTATGTAATTAC‑T‑AC‑AAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                                                <  2:943486/137‑1 (MQ=255)

                                                                                          aaacaaac‑ACGCCTGGGAAACCGGGCGT‑TTTTTTGTT‑C‑TTTTTTATGTAATTAC‑T‑AC‑AAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                                                <  2:4194424/140‑1 (MQ=255)

                                                                                          aaacaaac‑ACGCCTGGGAAACCGGGCGT‑TTTTTTGTT‑C‑TTTTTTATGTAATTAC‑T‑AC‑AAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                                                <  1:1406023/140‑1 (MQ=255)

                                                                                          aaacaaac‑ACGCCTGGGAAACCGGGCGT‑TTTTTTGTT‑C‑TTTTTTATGTAATTAC‑T‑AC‑AAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                                                <  1:1978613/140‑1 (MQ=255)

                                                                                          aaacaaac‑ACGCCTGGGAAACCGGGCGT‑TTTTTTGTT‑C‑TTTTTTATGTAATTAC‑T‑AC‑AAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                                                <  1:2887597/140‑1 (MQ=255)

                                                                                          aaacaaac‑ACGCCTGGGAAACCGGGCGT‑TTTTTTGTT‑C‑TTTTTTATGTAATTAC‑T‑AC‑AAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                                                <  1:2379900/140‑1 (MQ=255)

                                                                                          aaaCAAAA‑ACGCCTGGGAAACCGGGCGT‑TTTTTTGTT‑G‑TTTTTTATGTAATTAA‑T‑AA‑AAAATTTAAAAAAAAGGGAAAAACATCCCAATTACTTGCCAAATATTTTTTGGTAATTTTATGTAGGTAAAATTTGTATTTgg                                                >  2:2379900/1‑140 (MQ=255)

                                                                                          aaaCAAAA‑ACGCCTGGGAAACCGGGCGT‑TTTTTTGTT‑C‑TTTTTTATGTAATTAC‑T‑AC‑AAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                                                <  2:4777695/140‑1 (MQ=255)

                                                                                          aaaCAAAA‑ACGCCTGGGAAACCGGGCGT‑TTTTTTGTT‑C‑TTTTTTATGTAATTAC‑T‑AC‑AAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                                                <  2:3064426/140‑1 (MQ=255)

                                                                                          aaaCAAAA‑ACGCCTGGGAAACCGGGCGT‑TTTTTTGTT‑C‑TTTTTTATGTAATTAC‑T‑AC‑AAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                                                <  2:154463/140‑1 (MQ=255)

                                                                                          aaaCAAAA‑ACGCCTGGGAAACCGGGCGT‑TTTTTTGTT‑C‑TTTTTTATGTAATTAC‑T‑AC‑AAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                                                <  2:1716296/140‑1 (MQ=255)

                                                                                          aaaCAAAA‑ACGCCTGGGAAACCGGGCGT‑TTTTTTGTT‑C‑TTTTTTATGTAATTAC‑T‑AC‑AAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                                                <  2:1774745/140‑1 (MQ=255)

                                                                                          aaaCAAAA‑ACGCCTGGGAAACCGGGCGT‑TTTTTTGTT‑C‑TTTTTTATGTAATTAC‑T‑AC‑AAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                                                <  2:1974181/140‑1 (MQ=255)

                                                                                          aaaCAAAA‑ACGCCTGGGAAACCGGGCGT‑TTTTTTGTT‑C‑TTTTTTATGTAATTAC‑T‑AC‑AAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                                                <  2:2157775/140‑1 (MQ=255)

                                                                                          aaaCAAAA‑ACGCCTGGGAAACCGGGCGT‑TTTTTTGTT‑C‑TTTTTTATGTAATTAC‑T‑AC‑AAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                                                <  2:2317719/140‑1 (MQ=255)

                                                                                           aaCAAAACACGCCTGGGAAACCGGGCGT‑TTTTTTGTT‑C‑TTTTTTATGTAATTAC‑T‑AC‑AAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                                                <  1:211437/140‑1 (MQ=255)

                                                                                           aaCAAAA‑ACGCCTGGGAAACCGGGCGT‑TTTTTTGTT‑CTTTTTTTATGTAATTAC‑T‑AC‑AAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                                                <  2:49003/140‑1 (MQ=255)

                                                                                             cAAAA‑ACGCCTGGGAAACCGGGCGT‑TTTTTGGTT‑C‑TTTTTTATGTAATTAC‑T‑AC‑AAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGCTAATTTTATGTAGGTAAAATTTGTATTTGGCCg                                             <  2:4565239/140‑1 (MQ=255)

                                                                                             cAAAA‑ACCCCTGCGAAACCGGGAGT‑TTTTTTGTT‑C‑TTTTTAATGTAATTAC‑T‑AC‑AAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCg                                             <  2:1042704/140‑1 (MQ=255)

                                                                                               aaa‑aCGCCTGGGAAACCGGGCGT‑TTTTTTGTT‑C‑TTTTTTATGTAATTAC‑T‑AC‑AAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGtt                                           <  1:2461582/140‑1 (MQ=255)

                                                                                               aaa‑aCGCCTGGGAAACCGGGCGT‑TTTTTTGTT‑C‑TTTTTTATGTAATTAC‑T‑AC‑AAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGtt                                           <  1:3728181/140‑1 (MQ=255)

                                                                                                     cccTGGGAAACCGGGCGC‑TTTTTTTTT‑C‑TTTTTTATGTAATTAC‑T‑AC‑AAATTTGAAAAATACGGGAACACCATCCCATGTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCagag                                      <  1:532542/139‑1 (MQ=255)

                                                                                                      ccTGGGAAACCGGGCGT‑TTTTTTGTT‑C‑TTTTTTATGTAATTAC‑T‑AC‑AAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATGTGTATTTGGCCGTTCAgagg                                     <  1:2496994/140‑1 (MQ=255)

                                                                                                         gggAAACCGGGCGT‑GTTTTTGTT‑G‑TTTTTTTTGTAATTAC‑T‑AA‑AAATTTGAAAAAAACGGGAAAAAAATACCATTTTCTTACCAAAAATTTTATGGTAATTTTGTGTAGGTAAAATTTGTATTTTGGCGTTgagatgctg                                  >  2:1628618/1‑135 (MQ=255)

                                                                                                                    ttt‑ttttttttt‑C‑TTTTTTATTTAATTAC‑T‑AA‑AAATTTTAAAAATACGGTAAAAACATCCTATTTACTTCAAAAATTTTTGTTTTTAATTTTATTTAGTTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCggg                       <  2:1227600/138‑1 (MQ=255)

                                                                                                                    ctt‑ttttttGTT‑C‑TTTTTTATGTAATTAC‑T‑AC‑AAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCggg                       <  1:4143502/138‑1 (MQ=255)

                                                                                                                    cGT‑TTTTTTGTT‑C‑TTTTTTATGTAATTAC‑T‑AC‑AAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCggg                       <  2:1000701/140‑1 (MQ=255)

                                                                                                                    cGT‑TTTTTTGTT‑C‑TTTTTTATGTAATTAC‑T‑AC‑AAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCggg                       <  1:2176758/140‑1 (MQ=255)

                                                                                                                    cGT‑TTTTTTGTT‑C‑TTTTTTATGTAATTAC‑T‑AC‑AAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCggg                       <  1:4716459/140‑1 (MQ=255)

                                                                                                                    cGT‑TTTTTTGTT‑C‑TTTTTTATGTAATTAC‑T‑AC‑AAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCggg                       <  2:3381270/140‑1 (MQ=255)

                                                                                                                      t‑ttttttGTT‑C‑TTTTTTATGTAATTAC‑T‑AC‑AAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCGGGCa                     <  1:3361430/140‑1 (MQ=255)

                                                                                                                          ttttGTT‑C‑TTTTTTATGTAATTAC‑T‑AC‑AAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCGGGCAGGc                  <  2:1871861/140‑1 (MQ=255)

                                                                                                                            ttttt‑C‑TTTTTTATGTAATTAC‑T‑AC‑AAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCGGGCAGGCCt                <  2:791932/137‑1 (MQ=255)

                                                                                                                                t‑C‑TTTTTTATGTAATTAC‑T‑AC‑AAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCGGGCAGGCCTGAat            <  2:2169533/140‑1 (MQ=255)

                                                                                                                                           tGTAATTAC‑T‑AC‑AAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTTGCCGTTCCGAGGAGGTGAAAAGCGGGCCGGCCTGAATATTTTtata   >  2:2365176/1‑140 (MQ=255)

                                                                                                                                           tGTAATTAC‑T‑AC‑AAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCGGGCAGGCCTGAATATTTTtata   >  2:2634982/1‑140 (MQ=255)

CTCTAAAGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAA‑ACGCCTGGGAAACCGGGCGT‑TTTTTTGTT‑C‑TTTTTTATGTAATTAC‑T‑AC‑AAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCGGGCAGGCCTGAATATTTTTATAA  >  NZ_CP034943/2991109‑2991384

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`