BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	3,219,794	G→T	7.3%	V378F (GTT→TTT)	oxdC →	oxalate decarboxylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	3,219,794	0	G	T	7.3%	569.2 / 4.4	241	V378F (GTT→TTT)	oxdC	oxalate decarboxylase
Reads supporting (aligned to +/- strand): ref base G (125/86); new base T (2/27); total (128/113)
Fisher's exact test for biased strand distribution p-value = 3.62e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 241 total aligned reads displayed.

AATCTTTAAAGACGATCATTATGCCGATGTATCTTTAAACCAATGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGTTGTAAAAAAGAAATGCA‑GTAAATAAAAAACGACCAGCTTGCAGAGAGCAATCGTTCTCTGCAAGCCTTTATAAGGAGATGTACCAGTGAAACATCAAATTTCTTCAAAACGCTTATTGCGGCTCTTTATAAAGT  >  NZ_CP034943/3219661‑3219929

    tttAAAGACGATCATTATGCCGATGTATCTTTAAACCAATGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGAATCTTGATTTAGGAAAAGACTTTAATGATATTCTTTCGAAATAAAAGAATCCGGTTGTaaaaaa                                                                                                                                >  1:2688337/1‑140 (MQ=255)

             gATCATTATGCCGATGTATCTTTAAACCAATGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGTTGTAAAAAAGAAATGCA‑g                                                                                                                      >  1:4912134/1‑140 (MQ=255)

             gATCATTATGCCGATGTATCTTTAAACCAATGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGTTGTAAAAAAGAAATGCA‑g                                                                                                                      >  1:1791538/1‑140 (MQ=255)

              aTCATTATGCCGATGTATCTTTAAACCAATGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGTTGTAAAAAAGAAATGCA‑Gt                                                                                                                     >  2:800182/1‑140 (MQ=255)

               tCATTATGCCGATGTATCTTTAAACCAATGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGTTGTAAAAAAGAAATGCA‑Gta                                                                                                                    <  1:2485567/140‑1 (MQ=255)

               tCATTATGCCGATGTATCTTTAAACCAATGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGTTGTAAAAAAGAAATGCA‑Gta                                                                                                                    <  1:2935576/140‑1 (MQ=255)

                       ccGATGTATCTTTAAACCAATGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCGTGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGTTTTAAAAAAGAAATGCA‑GTAAATaaaaa                                                                                                            >  1:3421680/1‑140 (MQ=255)

                          aTGTATCTTTAAACCAATGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGTTGTAAAAAAGAAATGCA‑GTAAATAAAAAACg                                                                                                         >  2:597353/1‑140 (MQ=255)

                          aTGTATCTTTAAACCAATGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGTTGTAAAAAAGAAATGCA‑GTAAATAAAAAACg                                                                                                         >  1:1935928/1‑140 (MQ=255)

                          aTGTATCTTTAAACCAATGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGTTGTAAAAAAGAAATGCA‑GTAAATAAAAAACg                                                                                                         >  1:1561431/1‑140 (MQ=255)

                          aTGTATCTTTAAACCAATGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGTTGTAAAAAAGAAATGCA‑GTAAATAAAAAACg                                                                                                         >  1:2113017/1‑140 (MQ=255)

                          aTGTATCTTTAAACCAATGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGTTGTAAAAAAGAAATGCA‑GTAAATAAAAAACg                                                                                                         >  2:2332210/1‑140 (MQ=255)

                          aTGTATCTTTAAACCAATGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGTTGTAAAAAAGAAATGCA‑GTAAATAAAAAACg                                                                                                         >  2:617375/1‑140 (MQ=255)

                          aTGTATCTTTAAACCAATGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGTTGTAAAAAAGAAATGCA‑GTAAATAAAAAACg                                                                                                         >  2:2110117/1‑140 (MQ=255)

                          aTGTATCTTTAAACCAATGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGTTGTAAAAAAGAAATGCA‑GTAAATAAAAAACg                                                                                                         >  1:837728/1‑140 (MQ=255)

                           tttataTTTAAACAATTGGGTTCCATTGCTCCCTCAACCATTATTTAAAACGCATATTTATTTAGCAAAAGAATTAACTGATATTCTTTCGAAAGAAAAGCATCCGTTTGAAAAAAAGAAAGGCA‑GTAAATAAAAAACGa                                                                                                        <  2:2791893/138‑1 (MQ=255)

                           tGTATTTTTAAAACAATGGATTGAATTGTTTCCTGAATCTTTTGTAAAACAGCATGTTGATTTAGCAAAATACTTTACTGATTTTCTTTCTAAAGAAAAGCATCCGGTTGTAAAAAAGAAATGCA‑TTAAATAAAACACGa                                                                                                        <  2:2877581/140‑1 (MQ=255)

                           tGTATCTTTAAACCAATGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAGGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGTTGTAAAAAAGAAATGCA‑GTAAATAAAAAACGa                                                                                                        >  2:26299/1‑140 (MQ=255)

                           tGTATCTTTAAACCAATGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGTTGTAAAAAAGAAATGCA‑GTAAATAAAAAACGa                                                                                                        >  2:470857/1‑140 (MQ=255)

                           tGTATCTTTAAACCAATGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGTTGTAAAAAAGAAATGCA‑GTAAATAAAAAACGa                                                                                                        >  2:2414724/1‑140 (MQ=255)

                           tGTATCTTTAAACCAATGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGTTGTAAAAAAGAAATGCA‑GTAAATAAAAAACGa                                                                                                        >  2:4793156/1‑140 (MQ=255)

                           tGTATCTTTAAACCAATGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCTGTCGAAAGAAAAGCAAACGGTTGTAAAAAAGAAATGCA‑GTAACTAAAAAACGa                                                                                                        >  1:2919042/1‑140 (MQ=255)

                           tGTATCTTTAAACCAATGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGAATTAGGAAAAGACTTTACTGATATTCTTACCAAATAAAAGCATCAGGATGTAAAAAAGAAATCCA‑GTAAATAAAAAACGa                                                                                                        >  2:4948267/1‑140 (MQ=255)

                                ttttAAACAAAGGGTTTTAATTGATTCCTTAAACATTAGTTCAAAATCATTTTTATTTTGTAAAAGATTTTTCTGATTTTATTTCGACAGAAAATCATCGGTTTTTAAAAAAGAAATGCA‑TTAAATAAAAAACGACCAGc                                                                                                   <  2:2688337/139‑1 (MQ=255)

                                cTTTAAACAAATGTCTTGAAATGCTTCCTGAAAAATTATTTAAACACAATATTTATTTAGTAAAAGACTTTACTGAAATTCTTTCTAAAGAAAAGCAACCGGTTTTAAAAAATAAATCCA‑TTAAATAAAAAACGACCAGc                                                                                                   <  2:1208356/140‑1 (MQ=255)

                                atttAAACCAATGGCTTGCAATGCTTCCTGAAACATTATTTCAACAGCATCTTGATTTAGTAAAAGACTTTACTGTTATTCTTTCGAAAGAAAAGCATCCGGTTGTAAAAAAGATATGCA‑GTAAATAAAAAACGACCAGc                                                                                                   <  2:3227661/139‑1 (MQ=255)

                                attaaaaCCAATGTTTTCCATTTCTTCCTTAAACATTATTTCAAAACAATATTTTTTTAGAAAAAGAATTTACTTATTTTCTTAAGAAACAAAACCATCCGGTTTTAAAAAACAATTCCA‑TTAAATAAAAAACGACCAGc                                                                                                   <  2:1843118/136‑1 (MQ=255)

                                         aaTGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGTTGTAAAAAAGAAATGCA‑GTAAATAAAAAACGACCAGCTTGCagaga                                                                                          >  2:3942797/1‑140 (MQ=255)

                                          aTGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGTTGTAAAAAAGCAATGCA‑GTAAATAAAATACGACCAGCTTGCagagag                                                                                         >  1:36319/1‑140 (MQ=255)

                                          aTGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGTTGTAAAAAAGAAATGCA‑GTAAATAAAAAACGACCAGCTTGCagagag                                                                                         >  1:773773/1‑140 (MQ=255)

                                          aTGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGTTGTAAAAAAGAAATGCA‑GTAAATAAAAAACGACCAGCTTGCagagag                                                                                         >  2:2042449/1‑140 (MQ=255)

                                          aTGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGTTGTAAAAAAGAAATGCA‑GTAAATAAAAAACGACCAGCTTGCagagag                                                                                         >  2:2205864/1‑140 (MQ=255)

                                          aTGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGTTGTAAAAAAGAAATGCA‑GTAAATAAAAAACGACCAGCTTGCagagag                                                                                         >  2:4049164/1‑140 (MQ=255)

                                          aTGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGTTGTAAAAAAGAAATGCA‑GTAAATAAAAAACGACCAGCTTGCagagag                                                                                         >  1:817734/1‑140 (MQ=255)

                                          aTGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGTTGTAAAAAAGAAATGCA‑GTAAATAAAAAACGACCAGCTTGCagagag                                                                                         >  1:411258/1‑140 (MQ=255)

                                           tGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGTTGTAAAAAAGAAATGCA‑GTAAATAAAAAACGACCAGCTTGCAGAGAGc                                                                                        >  1:242604/1‑140 (MQ=255)

                                             gCTTGAAATGCTTCCTGAAAAATTAGTACAACAGCATCTTTATTTTTTAAAAGACTTTAATGATATTCTTTCCAAACAAAACCATCCGGTTTTAAAAAATAAATGCA‑GAAAATAAAAAACGACCAGCTTGCAGAGAGCaa                                                                                      <  2:2005439/140‑1 (MQ=255)

                                             gCTGGCATTGCTTCCTGAAACATTAGTACAACAGCTTCTTGTTTTAGGAAAAGACTTTACTGATTTTCTTTCGAAAGAAAAGCACCCGGTTGTAAAAAATAAATGCA‑GTAATTAAAACACGACCAGCTTGCAGAGAGCaa                                                                                      <  2:2743793/140‑1 (MQ=255)

                                             gCTGGCAATGATTCCTCAAAAATTATTTCAACAGCATCTTTATTTAGTAAAACACTTTACTGATATTCTTTCCAAAGAAAAGCATCCGGTTTTAAAAAAGAAATGCA‑GTAAATAAAAAACGACCAGCTTGCAGAGAGCaa                                                                                      <  2:3086871/140‑1 (MQ=255)

                                                  cattggttCCTGAAACATTCGTTCAACCGCTTCTTGATTTCGGAAAAGACTTTACTGTTATGGTTTCGAAACAAAAGCAGCCGGTTTTAAAAAATAACTGCT‑GTAAATAAAACACGACCAGCTTGCAGAGAGCAATCGtt                                                                                 <  2:424906/134‑1 (MQ=255)

                                                  cAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGTTGTAAAAAAGAAATGCA‑GTAAATAAAAAACTACCAGCTTGCAGAGAGCAATCGtt                                                                                 >  1:4373720/1‑140 (MQ=255)

                                                  cAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGTTGTAAAAAAGAAATGCA‑GTAAATAAAAAACGACCAGCTTGCAGAGAGCACTCGtt                                                                                 >  1:3408418/1‑140 (MQ=255)

                                                  cAATGCTTCCTGAAAATTTAGTTCAACAGCATCTTGATTTAGGAAAATACTTTACTTATATTCTTTCGAAAGAAAAGCATCCGGTTGTAAAAAAGAAATGCA‑GTAAATAAAAAACGACCAGCTTGCAGAGAGCAATCGtt                                                                                 <  2:3516468/140‑1 (MQ=255)

                                                  cAATGCTTCCTCAAACATTTTTTAAACAGCAACTTTATTTAGCAAAAGAATTTACTGATATTCTTTCTAACTAAAAGAATCCGGTTGTAAAAAAGAAAGGCA‑GTAAATAAAAAACGACCAGCTTGCAGAGAGCAATCGtt                                                                                 <  2:2142711/140‑1 (MQ=255)

                                                  cAATGCTTCCTCAAACATTATTTAAACAGCATCTTTTTTTAGCAAAAGACTTTACTGATATTCTTCCTAAACAAAAGCATCCGGTTGTAAAAAACAAATGCA‑GTAAATAAAAAACGACCAGCTTGCAGAGAGCAATCGtt                                                                                 <  2:872053/140‑1 (MQ=255)

                                                  cAATGCTTCCTCAAAAATTAGTAAAACAGCATCTTTATTTCCCAAAACAATTCACTGATATTCTTACGAAATAAAACAAACCGGTTGAAAAAAAGAAATGCA‑GAAAATAAAAAACGACCAGCTTGCAGAGAGCAATCGtt                                                                                 <  2:573990/140‑1 (MQ=255)

                                                  cAATGATTCATTAAACTTTAGTACAACATAATATTTATTTAGCAAAATATTTTACTCATATACTTTCTAAACAAAAGCATCCGTTTGTAAAAAACAAATGAA‑TTAAATAAAAAACGACCAGCTTGCAGAGAGCAATCGtt                                                                                 <  2:3766827/140‑1 (MQ=255)

                                                  ataTGCTTCATCAAAATTTAGTTCAACAGAATCTTGTTTTTTCAATCTACTTTACTGTTATTATTTGGAAAGAAATGCTTCCGTTTGTAAAAAACAAATGCA‑GAAAATAAAACACGACCAGCTTGCAGAGAGCAATCGtt                                                                                 <  2:311946/138‑1 (MQ=255)

                                                  aaaTGCTTCCTCAAACATTATTTCAAAAGCTTTTTTATTAAGTAAAAGAATTTACTTATATCATTTCCAAAGAAAAGCAGCGTGTTTAAAAAAAAAAATCAA‑TTAAATAAAAAACGACCAGCTTGCAGAGAGCAATCGtt                                                                                 <  2:715443/139‑1 (MQ=255)

                                                       cttacttaAACATTAGTTAAAAAGAATCTTTATTTATGAAAATAATTTATTTATATGCTTTCCAAATAAACGAATCCTGTTTTAAAAAATAAATGCA‑GTAAATAAAAAACGACCAGCTTGCAGAGAGCAATCGTTCTCTg                                                                            <  2:3174618/140‑1 (MQ=255)

                                                       cTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGTTGTAAAAAAGAAATGCA‑GTAAATAAAAAACGACCAGCTTGCAGAGAGCAATCGTTCTCTg                                                                            >  2:2119/1‑140 (MQ=255)

                                                       cTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGTTGTAAAAAAGAAATGCA‑GTAAATAAAAAACGACCAGCTTGCAGAGAGCAATCGTTCTCTg                                                                            >  2:2163327/1‑140 (MQ=255)

                                                       cTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATAAGTTTGTAAAAAAGAAATGCA‑GTAAATAAAAAACGACCATCTTGCATAGAGCAATCGTTCTCTg                                                                            >  2:1264173/1‑140 (MQ=255)

                                                       cTTCCTGAAACATTAGTTCAAAAGAAGCTTGAATTAGGAAAAGACGTTACTGATATTCTTTCTAAAGAAAAGCAACCGGATGTAAAAAAGAAATGCA‑GTAAATAAAAAACGACCACATTGAAGAGAGAAATCTTTctctc                                                                            >  1:320551/1‑139 (MQ=255)

                                                        ttCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGTTGTAAAAAAGAAATGCA‑GTAAATAAAAAACGACCAGCTTGCAGAGAGCAATCGTTCTCTGc                                                                           >  2:3666355/1‑140 (MQ=255)

                                                        gtcataaaaaATTAGTTAAACAGTTTTTTTTTTTCCAAAAAGATTTTCCTTCTTTTCTTTATATAAAAAAGC‑TCCGGTTTTTAAAAAACAATGCATTTAAAAAAAACACGACCAGCTTGCAGAGAGCAATCGTTCTCTGc                                                                           <  1:3817901/134‑1 (MQ=255)

                                                         tacttaaaCTTTAGTAAAACAGCATCTTTATTTAGTAAAATAGTTTACTGATTTTTTTTCGAAATAAACCCATCGGGTTTTAAAAAATAATTGCA‑GTAAATAAAAAACGACCAGCTTGCAGAGAGCAATCGTTCTCTGCa                                                                          <  2:4857892/135‑1 (MQ=255)

                                                         tCCTTAAACATTATTAAAAAACAATTTTGATTTAGCAAAATATTTTACTTATATTTTTTCTAAACAAAAGACCCCGGTTTAAAAAAACAAATCCA‑TAAAATAAAACACGACCAGCTTGCAGAGAGCAATCGTTCTCTGCa                                                                          <  1:1493875/140‑1 (MQ=255)

                                                         tCCTGAAAAATTAGTTCAACACCATCTTGATTTAGTAAAAGAATTTAATTATATTCTTTCTAAAGAAAAGAATCCGGTTGTAAAAAATAAATGAA‑GTAAATAAAAAACGACCAGCTTGCAGAGAGCAATCGTTCTCTGCa                                                                          <  2:3379502/140‑1 (MQ=255)

                                                         tCCTAAAAAATTAGTACAACAGGATCTTGATTTACCAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCAGCCGGTTGTAAAAAACAAATGCA‑GTAAATAAAAAACGACCAGCTTGCAGAGAGCAATCGTTCTCTGCa                                                                          <  2:4137579/140‑1 (MQ=255)

                                                          ccTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGTTGTAAAAAAGAAATGCA‑GTAAATAAAAAACGACCAGCTTGCAGAGAGCAATCGTTCTCTGCaa                                                                         >  2:1480531/1‑140 (MQ=255)

                                                           cttaaaaATTAGTTAAACAGATTCTTTATTTATTAAAATAATTTACTGATATTCTTTCTAAATAAAAGCATCCGGTTTTAAAAAATAAATGCA‑GTAAATAAAACACGACCAGCTTGCAGAGAGCAATCGTTCTCTGCAAg                                                                        <  2:1096693/137‑1 (MQ=255)

                                                               aaCATTAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGTTGTAAAAAAGAAATGCA‑GTAAATAAAAAACGACCAGCTTGCAGAGAGCAATCGTTCTCTGCAAGCCtt                                                                    >  1:1050897/1‑140 (MQ=255)

                                                               aaCATTAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGTTGTAAAAAAGAAATGCA‑GTAAATAAAAAACGACCAGCTTGCAGAGAGCAATCGTTCTCTGCAAGCCtt                                                                    >  2:466830/1‑140 (MQ=255)

                                                                 cATTAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGTTGTAAAAAAGAAATGCA‑GTAAATAAAAAACGACCAGCTTGCAGAGAGCAATCGTTCTCTGCAAGCCTTta                                                                  >  2:2974097/1‑140 (MQ=255)

                                                                   ttATTTAAACAGAATTTTTATTTAGTAAAATAATTTACTGATATTCTTTCGAAACAAAAGCATCGGGTTTTAAAAAATAAATGCA‑TTAAATAAAAAACGACCAGCTTGCAGAGAGCAATCGTTCTCTGCAAGCCTTtata                                                                <  2:1935928/140‑1 (MQ=255)

                                                                   ttAGTTAAACAGAATGTTTATTTTCTAAAATATTTTATTTATATTCTTTCTAAACAAAAGACTGCGGTTTAAAAAAAGAAATGCT‑TTAAATAAAACACGACCAGCTTGCAGAGAGCAATCGTTCTCTGCAAGCCTTtata                                                                <  1:3156577/140‑1 (MQ=255)

                                                                   ttAGTCCAACCGAATGTTTATTTCGGAAACAACATTCCTCATATTCTTTCTAACTAAAAGCATCCGGTTTTAAAAAACAAATGCA‑CTAAATAAAACACGACCAGCTTGCAGAGAGCAATCGTTCTCTGCAAGCCTTtata                                                                <  1:1224221/140‑1 (MQ=255)

                                                                    tAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGTTGTAAAAAAGAAATGCA‑GTAAATAAAAAACGACCAGCTTGCAGAGAGCAATCGTTCTCTGCAAGCCTTTATaa                                                               >  2:3092521/1‑140 (MQ=255)

                                                                    tAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGTTGTAAAAAAGAAATGCA‑GTAAATAAAAAACGACCAGCTTGCAGAGAGCAATCGTTCTCTGCAAGCCTGTATaa                                                               >  2:2454149/1‑140 (MQ=255)

                                                                    tAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATACTTTCGAAAGAAAAGCATCCGGTTGTAAAAAAGAAATGCA‑GTAAATAAAAAACGACCAGCTTGCAGAGAGCAATCGTTATCTGCAAGCCTTTATaa                                                               >  2:4524761/1‑140 (MQ=255)

                                                                        tCAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGTTGTAAAAAAGAAATGCA‑GTAAATAAAAAACGACCAGCTTGCAGAGAGCAATCGTTCTCTGCAAGCCTGTATAAGgag                                                           >  1:3567489/1‑140 (MQ=255)

                                                                           aCAGAATCTTGATTTAGCAAAATAATTTACTTATATTCTTTCTAAACAAAAGCATCGGTTTTTAAAAAAGAAATGAA‑TTAAATAAAAAACGACCAGCTTGCAGAGAGCAATCGTTCTCTGCAAGCCTTTATAAGGAGATg                                                        <  2:1911485/140‑1 (MQ=255)

                                                                            cAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGTTGTAAAAAAGAAATGCA‑GTAAATAAAAAACGACCAGCTTGCAGAGAGCAATCGTTCTCTGCAAGCCTTTATAAGGAGATGt                                                       >  1:1242845/1‑140 (MQ=255)

                                                                               cATCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGTTGTAAAAAAGAAATGCA‑GTAAATAAAAAACGACCAGCTTGCAGAGAGCAATCGTTCTCTGCAAGCCTTTCTAAGGAGATGTAcc                                                    >  2:3030202/1‑140 (MQ=255)

                                                                                 tCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGTTGTAAAAAAGAAATGCA‑GTAAATAAAAAACGACCAGCTTGCAGAGAGCAATCGTTCTCTGCAAGCCTTTATAAGGAGATGTACCAg                                                  >  2:2907495/1‑140 (MQ=255)

                                                                                    tGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGTTGTAAAAAAGAAATGCA‑GTAAATAAAAAACGACCAGCTTGCAGAGAGCAATCGTTCTCTGCAAGCCTTTATAAGGAGATGTACCAGTGa                                               >  2:3352117/1‑140 (MQ=255)

                                                                                    tGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGTTGTAAAAAAGAAATGCA‑GTAAATAAAAAACGACCAGCTTGCAGAGAGAAATCGTTCTCTGCAAGACTTTATAAGTAGAAGTACCAgaga                                               >  2:2405377/1‑140 (MQ=255)

                                                                                                      tACTGATATTCTTTCGAAAGAAAAGCATCCGGTTGTAAAAAAGAAATGCA‑GTAAATAAAAAACGACCAGCTTGTAGAGAGCAATCGTTCTCTGCAAGCCTTTATAAGGAGATGTACCAGTGAAACATCAAATTTCTTCaa                             >  1:1989380/1‑140 (MQ=255)

                                                                                                            tatTCTTTCGAAAGAAAAGCATCCGGTTGTAAAAAAGAAATGCA‑GTAAATAAAAAACGACCAGCTTGCAGAGAGCAATCGTTCTCTGCAAGCCTTTATAAGGAGATGTACCAGTGAAACATCAAATTTCTTCAAAACGCt                       >  1:3336026/1‑140 (MQ=255)

                                                                                                            tatTCTTTCGAAAGAAAAGCATCCGGTTGTAAAAAAGAAATGCA‑GTAAATAAAAAACGACCAGCTTGCAGAGAGCAATCGTTCTCTGCAAGCCTTTATAAGGAGATGTACCAGTGAAACATCAAATTTCTTCAAAACGCt                       >  1:925670/1‑140 (MQ=255)

                                                                                                            tatTCTTTCGAAAGAAAAGCATCCGGTTGTAAAAAAGAAATGCA‑GTAAATAAAAAACGACCAGCTTGCAGAGAGCAATCGTTCTCTGCAAGCCTTTATAAGGAGATGTACCAGTGAAACATCAAATTTCTTCAAAACGCt                       >  1:945268/1‑140 (MQ=255)

                                                                                                            tatTCTTAAGAAACAAAAGCATCCGGTTGTAAAAAATAAATGAA‑GTAAATAAAAAACGACCAGCTTGCAGAGAGCAATCGTTCTCTGCAAGCCTTTATAAGGAGATGTACCAGTGAAACATCAAATTTCTTCAAAACGCt                       <  2:2582626/140‑1 (MQ=255)

                                                                                                            tatGCTTGCGAAAGAAAAGCATCCGGTTTAAAAAAAGAAAGCCA‑TTAAATAAAAAACGACCAGCTTGCAGAGAGCAATCGTTCTCTGCAAGCCTTTATAAGGAGATGTACCAGTGAAACATCAAATTTCTTCAAAACGCt                       <  2:3808297/140‑1 (MQ=255)

                                                                                                                   tCGAAAGAAAAGCATCCGGTTGTAAAAAAGAAATGCA‑GTAAATAAAAAACGACCAGCTTGCAGAGAGCAATCGTTCTCTGCAAGCCTTTATAAGGAGATGTACCAGTGAAACATCAAATTTCTTCAAAACGCTTATTGCg                >  1:4405555/1‑140 (MQ=255)

                                                                                                                   tCGAAAGAAAAGCATCCGGTTGTAAAAAAGAAATGCA‑GTAAATAAAAAACGACCAGCTTGCAGAGAGCAATCGTTCTCTGCAAGCCTTTATAAGGAGATGTACCAGTGAAACATCAAATTTCTTCAAAACGCTTATTGCg                >  1:282184/1‑140 (MQ=255)

                                                                                                                     gaaagaaaAGCATCCGGTTGTAAAAAAGAATTGCA‑GTAAATAAAAAACGACCAGCTTGCAGAGAGCAATCGTTCTCTGCAAGCCTTTATAAGGAGATGTACCAGTGAAACATCAAATTTCTTCAAAACGCTTATTGCGGc              <  2:730009/140‑1 (MQ=255)

                                                                                                                      aaccaaaaCCACCCGGTTGAAAAAAACAAAGGCA‑GTAAATAAACCACGACCAGCTTGCAGAGAGCAATCGTTCTCTGCAAGCCTTTATAAGGAGATGTACCAGTGAAACATCAAATTTCTTCAAAACGCTTATTGCGGct             <  1:2344043/136‑1 (MQ=255)

                                                                                                                      aaagtaaATCATCCTGTTTTAATATATAAATGCA‑CTAAATAAAAAACGACCAGCTTGCAGAGAGCAATGGTTCTCTGCAAGCCTTTATAAGGAGATGTACCAGTGAAACATCAAATTTCTTCAAAACGCTTATTGCGGct             <  2:3030807/140‑1 (MQ=255)

                                                                                                                      aaagaaaagaATCCGGTTGTAAAAAAGAAATCCA‑GTAAATAAAAAACGACCAGCTTGCAGAGAGCAATCGTTCTCTGCAAGCCTTTATAAGGAGATGTACCAGTGAAACATCAAATTTCTTCAAAACGCTTATTGCGGct             <  2:2473320/140‑1 (MQ=255)

                                                                                                                      aaagaaaAGCATCCGGTTTTAAAAAATAAATGCA‑GTAAATAAAACACGACCAGCTTGCAGAGAGCAATCGTTCTCTGCAAGCCTTTATAAGGAGATGTACCAGTGAAACATCAAATTTCTTCAAAACGCTTATTGCGGct             <  2:1590582/140‑1 (MQ=255)

                                                                                                                        ataaaaGCATCCTGTTTTAAAAAAGAATTCCA‑GTAAATAAAAAACGACCAGCTTGCAGAGAGCAATCGTTCTCTGCAAGCCTTTATAAGGAGATGTACCAGTGAAACATCAAATTTCTTCAAAACGCTTATTGCGGctct           <  2:3741632/138‑1 (MQ=255)

                                                                                                                        agaaaAGAATCCGGTTGTAAAAAAGAAATCCA‑GTAAATAAAACACGACCAGCTTGCAGAGAGCAATCGTTCTCTGCAAGCCTTTATAAGGAGATGTACCAGTGAAACATCAAATTTCTTCAAAACGCTTATTGCGGctct           <  2:1571836/140‑1 (MQ=255)

                                                                                                                          aaaaGCATCCGGTTGTAAAAAAGAAATCCA‑TTAAATAAAACACGACCAGCTTGCAGAGAGCAATCGTTCTCTGCAAGCCTTTATAAGGAGATGTACCAGTGAAACATCAAATTTCTTCAAAACGCTTATTGCGGCTCttt         <  1:4274297/140‑1 (MQ=255)

                                                                                                                          aaaaGAATCCGGTTGAAAAAAATAAATGCA‑GTAAATAAAAAACGACCAGCTTGCAGAGAGCAATCGTTCTCTGCAAGCCTTTATAAGGAGATGTACCAGTGAAACATCAAATTTCTTCAAAACGCTTATTGCGGCTCttt         <  1:4850806/140‑1 (MQ=255)

                                                                                                                             cgCAACCGGGTGAAAAAAAGACACCCA‑GTAAATAAACCACGCCCAGCTTGCAGAGAGCAATCGTTCTCTGCAAGCCTTTATAAGGAGATGTACCAGTGAAACATCAAATTTCTTCAAAACGCTTATTGCGGCTCTTtata      <  1:3778914/139‑1 (MQ=255)

                                                                                                                             accATCCGTGTTTAAAAAACAAATCAA‑CTAAATAAACCACGACCAGCTTGCAGAGAGCAATCGTTCTCTGCAAGCCTTTATAAGGAGATGTACCAGTGAAACATCAAATTTCTTCAAAACGCTTATTGCGGCTCTTtata      <  1:4801592/138‑1 (MQ=255)

                                                                                                                             aGCATCGGGTTTTAAAAAATAAATCCA‑TTAAATAAAACACGACCAGCTTGCAGAGAGCAATCGTTCTCTGCAAGCCTTTATAAGGAGATGTACCAGTGAAACATCAAATTTCTTCAAAACGCTTATTGCGGCTCTTtata      <  1:2585997/140‑1 (MQ=255)

                                                                                                                             aGCACCCGTTTCTAAAAAACAACTCAA‑TTAAATAAAAAACCACCAGCTTGCAGAGAGCAATCGTTCTCTGCAAGCCTTTATAAGGAGATGTACCAGTGAAACATCAAATTTCTTCAAAACGCTTATTGCGGCTCTTtata      <  1:4066813/140‑1 (MQ=255)

                                                                                                                                  ccGGTTGTAAAAAGGAAATGCA‑GTAAATAAAAAACGACCAGCTTGCAGAGAGCAATCGTTCTCTGCAAGc                                                                       >  1:3678712/1‑70 (MQ=255)

AATCTTTAAAGACGATCATTATGCCGATGTATCTTTAAACCAATGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGTTGTAAAAAAGAAATGCA‑GTAAATAAAAAACGACCAGCTTGCAGAGAGCAATCGTTCTCTGCAAGCCTTTATAAGGAGATGTACCAGTGAAACATCAAATTTCTTCAAAACGCTTATTGCGGCTCTTTATAAAGT  >  NZ_CP034943/3219661‑3219929

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`