BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	240,174	T→G	8.7%	N120H (AAC→CAC)	EO946_RS01360 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	240,174	0	T	G	8.7%	715.9 / 7.3	317	N120H (AAC→CAC)	EO946_RS01360	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (173/101); new base G (41/2); total (214/103)
Fisher's exact test for biased strand distribution p-value = 5.69e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 325 total aligned reads displayed.

GTCACGCGTGACAGTGCGGCTGTGGTGGAGAAGGTGTAAGAGAGAGAGAAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTA‑TTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCA  >  NZ_CP034943/240035‑240313

 tCACGCGTGACAGTGCGGCTGTGGTGGAGAAGGTGTAAGAGAGAGAGAAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTA‑TTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGgtt                                                                                                                                            >  2:4616520/1‑140 (MQ=255)

          aCAGTGCGGCTGTGGTGGAGAAGGTGTAAGAGAGAGAGAAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTA‑TTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGAtttt                                                                                                                                   >  1:4737394/1‑140 (MQ=255)

          aCAGTGCGGCTGTGGTGGAGAAGGTGTAAGAGAGAGAGAAGGAGCATCTCAGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGAAttt                                                                                                                                    >  1:4939602/1‑140 (MQ=255)

           cAGTGCGGCTGTGGTGGAGAAGGTGTAAGAGAGAGAGAAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTA‑TTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGAttttt                                                                                                                                  >  2:2942858/1‑140 (MQ=255)

           cAGTGCGGCTGTGGTGGAGAAGGTGTAAGAGAGAGAGAAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTA‑TTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTAGGAttttt                                                                                                                                  >  2:1708469/1‑140 (MQ=255)

           cAGTGCGGCTGTGGTGGAGAAGGTGTAAGAGAGAGAGAAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTA‑TTTTTGTTCTAATACGAAGTCGATCATTCCGAATATACGGGCGTTTTCGGAttttt                                                                                                                                  >  2:826155/1‑140 (MQ=255)

               gCGGCTGTGGTGGAGACGGTGTAAGAGAGAGAGAAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTA‑TTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTtttgttt                                                                                                                              <  2:2519459/140‑1 (MQ=255)

                   cTGTGGTGGCGAAGGTGTAAGAGAGAGAGAAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTA‑TTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAg                                                                                                                          >  1:4013442/1‑140 (MQ=255)

                   cTGTGGTGGAGAAGGTGTAAGAGAGAGAGAAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTA‑TTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAg                                                                                                                          >  2:3783697/1‑140 (MQ=255)

                      tggtggAGAAGGTGTAAGAGAGAGAGAAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTA‑TTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGttt                                                                                                                       >  1:4474249/1‑140 (MQ=255)

                        gtggAGAAGGTGTAAGAGAGAGAGAAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTA‑TTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAATttttt                                                                                                                     >  2:3679505/1‑139 (MQ=255)

                        gtggAGAAGGTGTAAGAGAGAGAGAAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTA‑TTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGttttt                                                                                                                     >  1:106155/1‑139 (MQ=255)

                        gtggAGAAGGTGTAAGAGAGAGAGAAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTA‑TTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTg                                                                                                                     >  2:4820453/1‑140 (MQ=255)

                        gtggAGAAGGTGTAAGAGAGAGAGAAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTA‑TTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTGTTAGTATTTTTGTTTGAAGttttt                                                                                                                     >  1:3245718/1‑139 (MQ=255)

                             gaAGGTGTAAGAGAGAGAGAAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTA‑TTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTGGGATTTTTGTTTGTAGTTTTggggtg                                                                                                                >  1:1675837/1‑137 (MQ=255)

                                  tgtAAGAGAGAGAGAAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTA‑TTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTAGGATTTTTTTTTTAAGTTTTTGGAGATTTGa                                                                                                           >  2:1460255/1‑140 (MQ=255)

                                  tgtAAGAGAGAGAGAAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGATGTTTTTGTA‑TTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGa                                                                                                           >  2:4939172/1‑140 (MQ=255)

                                   gtAAGAGAGAGAGAAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTA‑TTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTTTTTGAAGTTTTGGGAGATTTGaa                                                                                                          >  2:1908627/1‑140 (MQ=255)

                                   gtAAGAGAGAGAGAAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTA‑TTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGaa                                                                                                          >  2:879626/1‑140 (MQ=255)

                                   gtAAGAGAGAGAGAAGGAGCATCTCTGATGTTGGAATTATTCAGAGAGGCTGTTTTTGTA‑TTTTTGTTCTAATACTAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGaa                                                                                                          >  2:2870764/1‑140 (MQ=255)

                                   ggaaGAGAGAGAGAAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTA‑TTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTGTTCGGATTTTTTTTTTAAGTTTTGGGCGAGTTgga                                                                                                          >  2:2794371/3‑138 (MQ=255)

                                          agagagaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTA‑TTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTTAAGTTTTGGGAGATTTGAATGGGttt                                                                                                   >  2:242770/1‑140 (MQ=255)

                                          agagagaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTA‑TTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAAttgagtt                                                                                                   >  2:3143429/1‑134 (MQ=255)

                                           gagagaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTA‑TTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACTGGCGTTTTCGGATTTTTTTTTGAATTTTTTGGAGATTTGAATTGCtgtt                                                                                                  >  1:2305646/1‑140 (MQ=255)

                                            agagaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTA‑TTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTTTTTTAAGTTTTGGGAGATTTGAATGGCTTTTc                                                                                                 >  2:238221/1‑140 (MQ=255)

                                            agagaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTA‑TTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTTAAGTTTTTGGAGATTTTAATTGCTTTTc                                                                                                 >  1:5112/1‑140 (MQ=255)

                                            agagaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTA‑TTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTTAAGTTTTGGGAGATATGAATGGCtttta                                                                                                 >  2:3472006/1‑139 (MQ=255)

                                            agagaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTA‑TTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAATTTTTGGGAGATTTTAATTGCTTTTc                                                                                                 >  1:1194618/1‑140 (MQ=255)

                                            agagaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTA‑TTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTAGGATTTTTTTTTTAAGTTTTTGGAGATTTTAATTGCTTTTc                                                                                                 >  1:1527380/1‑140 (MQ=255)

                                            agagaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTA‑TTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTAGGATTTTTGTTTGAAATTTTTGGAGATTTTAATGGCTTTTc                                                                                                 >  1:912918/1‑140 (MQ=255)

                                            agagaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTA‑TTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTGTTGGGATTTTTGTTTGAAGTTTTGGGGGATTTGAATGGGTTTTc                                                                                                 >  1:1007400/1‑140 (MQ=255)

                                            agagaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTA‑TTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTGTTCGGATTTTTGTTTTAAGTTTTGGGAGATTTGAATGGCTTTTc                                                                                                 >  1:3730450/1‑140 (MQ=255)

                                            agagaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTA‑TTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTGTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTc                                                                                                 >  1:4359722/1‑140 (MQ=255)

                                            agagaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTA‑TTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTGTTAGGATTTTTTTTTTAAGTTTTTGGATATTTTAATGTCTTTTc                                                                                                 >  2:4166913/1‑140 (MQ=255)

                                            agagaAGGAGCATCTCTGATGGTGGAATTATTCAGAGATGCTGTTTTTGTA‑TTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTTAAGTTTTTGGAGAATTTAATTGCtttta                                                                                                 >  1:1635229/1‑139 (MQ=255)

                                                    aGCATCTCTGATGGTGGAATTATTCACCGATGGTGTGTTTGTA‑TTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTGTTCGGATTTTTGTTTGAAGTTTTTGGAGATTTGAATGGCTTTTCCGTTTTTa                                                                                         >  2:3801446/1‑140 (MQ=255)

                                                        tctcTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTA‑TTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAAc                                                                                     >  2:3373708/1‑140 (MQ=255)

                                                         ctctGATGTTGGAATTATTCAGAGATGCTGTTTTTGTA‑TTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACa                                                                                    <  1:3089206/140‑1 (MQ=255)

                                                         ctctGATGTTGGAATTATTCAGAGATGCTGTTTTTGTA‑TTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACa                                                                                    <  1:1752998/140‑1 (MQ=255)

                                                         ctctGATGTTGGAATTATTCAGAGATGCTGTTTTTGTA‑TTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACa                                                                                    <  1:4693899/140‑1 (MQ=255)

                                                           ctGATGTTGGAATTATTCAGAGATGCTGTTTTTGTA‑TTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAag                                                                                  >  2:3013945/1‑140 (MQ=255)

                                                              aTGTTGGAATTATTCAGAGATGCTGTTTTTGTA‑TTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTTTTTGAAGTTTTTGGTGATTTGAATGGCTTTTGCGTTTTTAAAAAAAGAgg                                                                               >  1:2792755/1‑140 (MQ=255)

                                                              aTGTTGGAATTATTCAGAGATGCTGTTTTTGTA‑TTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTACGTTTTTAAAACAAGAgg                                                                               >  1:1455407/1‑140 (MQ=255)

                                                              aTGTTGGAATTATTCAGAGATGCTGTTTTTGTA‑TTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTGTTAGGATTTTTTTTTGTAGTTTTGTGAGATTTTAATTGGTTTTGCCTTTTTAAAACAATAgg                                                                               >  1:1142609/1‑140 (MQ=255)

                                                                   ggAATTATTCAGAGATGCTGTTTTTGTA‑TTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTGGGATTTTTTTTTGTACTTTTTGGAGATTTGAATGGGGTTTACGTTTTTAAAACAATAGTCaaac                                                                          >  1:4433460/1‑138 (MQ=255)

                                                                   ggAATTATTCAGAGATGCTGTTTTTGTA‑TTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTTTTTTAATTTTTGGGATATTTTAATGGCTTTTCCGTTTTTTAAAAAAAAGGCAAGc                                                                          >  1:2903479/1‑140 (MQ=255)

                                                                   ggAATTATTCAGAGATGCTGTTTTTGTA‑TTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTTAAGTTTTGGGAGATTTGAATGGCTTTTACGTTTTTAAAAAAAGACGCAAGc                                                                          >  2:4460384/1‑140 (MQ=255)

                                                                   ggAATTATTCAGAGATGCTGTTTTTGTA‑TTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGTAGGTTTGGGAGATTTGAATTGCTTTTCCGTTTTTTAAAGAATAGGAAAGc                                                                          >  2:4075554/1‑140 (MQ=255)

                                                                   ggAATTATTCAGAGATGCTGTTTTTGTA‑TTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAATTTTTTGGCGTTTTGAATGGCTTTTACGTTGTTAAAACAAGAGGCAAGc                                                                          >  1:3175621/1‑140 (MQ=255)

                                                                   ggAATTATTCAGAGATGCTGTTTTTGTA‑TTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAATTTTTGGGAGATTTGAATGGCTTTTACGTTTTTAAAAAAAGATGCAAGc                                                                          >  2:2267818/1‑140 (MQ=255)

                                                                   ggAATTATTCAGAGATGCTGTTTTTGTA‑TTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTTAATTGCTTTTCCGTTTTTAAAACAAGAGGCAAGc                                                                          >  1:3598034/1‑140 (MQ=255)

                                                                   ggAATTATTCAGAGATGCTGTTTTTGTA‑TTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTAGGATTTTTTTTTTAACTTTTTGGAGATTTTAAATGCGTTTACGTTTTTAAAAAAAGATGCaatc                                                                          >  1:770652/1‑138 (MQ=255)

                                                                   ggAATTATTCAGAGATGCTGTTTTTGTA‑TTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTGTTCGGCTTTTTTTTTTTAGTTTTTGGAGATTTTAATTGCTTTTGCGTTTTTAAAAAAAGAGGCAAGc                                                                          >  1:342739/1‑140 (MQ=255)

                                                                   ggAATTATTCAGAGATGCTGTTTTTGTA‑TTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTGTTCGGATTTTTTTTTTTAGTTTTTGGAGATTTGAATTGCTTTTCCGTTTTTAAAAAAAGAGGCaatc                                                                          >  1:392302/1‑138 (MQ=255)

                                                                   ggAATTATTCAGAGATGCTGTTTTTGTA‑TTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTGTTCGGATTTTTGTTTTAAGTTTTGGGAGATTTTAATGGGTTTTACGTTTTTAAAACAAGAGGCAAGc                                                                          >  2:1642700/1‑140 (MQ=255)

                                                                   ggAATTATTCAGAGATGCTGTTTTTGTA‑TTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTGTTAGGATTTTTTTTTGAACTTTTTGGAGATTTTAAAGGCTTTTCCGTTTTTAAAAAAATAGGaaatc                                                                          >  1:3639551/1‑138 (MQ=255)

                                                                   ggAATTATTCAGAGATGCGGTTTTTGTA‑TTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTAGGATTTTTGTTTGTAGTTTTTGGAGATTTGAATGGCTTTTGCGTTTTTAGAACAATAGGCAAGc                                                                          >  1:2261835/1‑140 (MQ=255)

                                                                          ttCAGAGATGCTGTTTTTGTA‑TTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTTGGAGATTTGAATGGCTTTTACGTTTTTAAAACAAGAGGCAAGCCAGATaa                                                                   >  2:1179482/1‑140 (MQ=255)

                                                                               agaTGCTGTTTTTGTA‑TTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTGGGATTTTTTTTTTAAGTTTTTGGAGATTTTAATTGCTTTTCCGTTTTTAAAAAAAGAGGCAACCCAGATAAAcccc                                                              >  1:2570496/1‑139 (MQ=255)

                                                                                    cTGTTTTTTTA‑TTTTTGTTCTAATACGAAGTCGATCATTCCGACTATAAGGGCGTTTTCGGATTTTTTTTTGAAGTTTTTGGAGATTTGAATTGCTGTTCCGTTTTTTAAACAAGAGGCAAACCAGATAAAACAGacaac                                                         >  1:3071194/1‑140 (MQ=255)

                                                                                    cTGTTTTTGTA‑TTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTTTTTGAAGTTTTTGGAGTTTTGAATTTCGTTTCCGTTTTTTAAAAAACAGGCAACCCAGATAAACCCCacaac                                                         >  1:2994757/1‑140 (MQ=255)

                                                                                    cTGTTTTTGTA‑TTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTAGGATTTTTGTTTGAAGTTTTTGGGGATTTGAAATGGTTTTACGTTTTTTAAAAAAAAGTCAAGCCAGATAAAAACCacaac                                                         >  1:1370360/1‑140 (MQ=255)

                                                                                    cTGTTTTTGTA‑TTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTAGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATTGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCCacaac                                                         >  2:4334514/1‑140 (MQ=255)

                                                                                    cTGTTTTTGTA‑TTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTGTTCGGATTTTTGGTTGAAGTTTTTGGAGATTTGAATGGGATTTCCGTTTTTAAAAAAATAGGCAAGCCCGAGAAACCCCacaac                                                         >  1:689719/1‑140 (MQ=255)

                                                                                    cTGTTTTTGTA‑TTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTGTTCGGAATTTTTTTTGAAGTTTTGGGAGATTTGAAATGCGTTTACATTTTTAAAAAAAGAGGCAAGCCACATAAAACCGaaaaa                                                         >  1:2046654/1‑139 (MQ=255)

                                                                                       tttttGTA‑TTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTTGGAGATTTTAATTGCTTTTCCGTTTTTTAAAAAAGAGGCAAACCAGATAAAACCCacaaaaaa                                                      >  1:2639173/1‑139 (MQ=255)

                                                                                                  tttGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTTTTTTAACTTTTTGGAGATTTTAATTGCTTTTGCGTTTTTAAAAAAAGAGGCAAGCCAGAAAAAACCGACAAAAACAAAAaataaa                                            >  1:3499325/1‑139 (MQ=255)

                                                                                                  tttGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAaataat                                            <  2:1764499/140‑1 (MQ=255)

                                                                                                  tttGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTACGTTTTTAAAACAAGAGGCAAGCCAGATAAAACCGACAACAACAAAAaataat                                            >  2:2191250/1‑140 (MQ=255)

                                                                                                      ttCTACTACGAAGTCGATAATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTGTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAg                                        <  1:2385969/140‑1 (MQ=255)

                                                                                                      ttCGAATCCGACGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGCAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAg                                        <  2:3979574/140‑1 (MQ=255)

                                                                                                      gtCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAg                                        <  1:4119117/139‑1 (MQ=255)

                                                                                                      gtCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAg                                        <  2:1029349/139‑1 (MQ=255)

                                                                                                          catccGAAGTCGATCATTCCGACTATCCGGGCGTTTTCGGATTTTTGTTTGAAGTTGAGGGAGATTGGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACACAAAATAATCTAGCAAg                                    <  1:4845100/136‑1 (MQ=255)

                                                                                                           aTACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGt                                   <  2:76961/140‑1 (MQ=255)

                                                                                                           aTACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGt                                   <  1:603986/140‑1 (MQ=255)

                                                                                                            tACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTa                                  <  1:1880571/140‑1 (MQ=255)

                                                                                                             aCGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTaa                                 <  2:4946931/140‑1 (MQ=255)

                                                                                                                  gTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTcc                            <  1:1460255/140‑1 (MQ=255)

                                                                                                                  gTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTcc                            <  1:4514993/140‑1 (MQ=255)

                                                                                                                   tCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCca                           >  2:4245570/1‑140 (MQ=255)

                                                                                                                   tCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTACGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCca                           >  2:3064820/1‑140 (MQ=255)

                                                                                                                      aTCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGGTTTTACGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCcatgc                        >  2:2449039/1‑140 (MQ=255)

                                                                                                                      aTCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCcatgc                        <  2:2586231/140‑1 (MQ=255)

                                                                                                                      aTCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCcatgc                        <  2:1533859/140‑1 (MQ=255)

                                                                                                                       tccttCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTGGAATGGCTTTTCCGTTTTTAAAACAAGAGGAAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCcatgca                       <  1:2526183/137‑1 (MQ=255)

                                                                                                                       tCATTCCGACTATACGGGCGTTTTCGTATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCcatgca                       <  1:2449620/140‑1 (MQ=255)

                                                                                                                       tCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCcatgca                       <  1:854151/140‑1 (MQ=255)

                                                                                                                       tCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCcatgca                       <  1:4198048/140‑1 (MQ=255)

                                                                                                                       tCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCcatgca                       <  1:3783697/140‑1 (MQ=255)

                                                                                                                       tCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCcatgca                       <  1:1707815/140‑1 (MQ=255)

                                                                                                                       tCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCcatgca                       <  2:70137/140‑1 (MQ=255)

                                                                                                                       tCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCcatgca                       <  1:2669889/140‑1 (MQ=255)

                                                                                                                           tCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATggg                   <  1:4075644/140‑1 (MQ=255)

                                                                                                                             cGACTATACGGGCGTTTGCGGATTTTTGTTTGAAGTTTTGTGAGAGTTGAATGGCTTTTCCGTTTTTAAAAAAATAGGCAAGCCAGAGAAAAACCACAACAACAAAAAATAATAAAGAAACTAAACACCATGCATGGGGt                 >  2:125828/1‑140 (MQ=255)

                                                                                                                                   taCGGGCGTGTTCGGATTTTTTTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAAACCGACAACAACAAAAAATAATCTAGCAACTAAAAACCAAGCATGGGGTAattca           >  2:1341333/1‑140 (MQ=255)

                                                                                                                                     cGGGCGTTTTCTGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAattcatt         <  2:2678578/140‑1 (MQ=255)

                                                                                                                                           ttttCGGATTTTTGTTTGTAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGc   >  2:4962177/1‑140 (MQ=255)

                                                                                                                                            tttCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCa  <  2:2048023/140‑1 (MQ=255)

                                                                                                                                            tttCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCa  <  2:1124364/140‑1 (MQ=255)

GTCACGCGTGACAGTGCGGCTGTGGTGGAGAAGGTGTAAGAGAGAGAGAAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTA‑TTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAGTTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAGAGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCA  >  NZ_CP034943/240035‑240313

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`