BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	3,851,254	G→C	5.1%	L41V (CTT→GTT)	EO946_RS19820 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	3,851,254	0	G	C	5.1%	809.5 / 3.1	385	L41V (CTT→GTT)	EO946_RS19820	hypothetical protein
Reads supporting (aligned to +/- strand): ref base G (247/101); new base C (3/24); total (260/125)
Fisher's exact test for biased strand distribution p-value = 7.84e-10
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 400 total aligned reads displayed.

TGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTAT‑GCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATAAAATCTTCCCCTTATCAG  >  NZ_CP034943/3851118‑3851393

tGTAATATGGATTTGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTAT‑GCAAGccc                                                                                                                                          <  2:2853674/140‑1 (MQ=255)

tGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTCTCCTGATTCGATCGTAT‑GCAAGccc                                                                                                                                          >  1:1865240/1‑140 (MQ=255)

tGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATTATTCGATCGTAT‑GCAAGccc                                                                                                                                          >  1:4919346/1‑140 (MQ=255)

tGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTAT‑GCAAg                                                                                                                                             >  1:1027349/1‑137 (MQ=255)

tGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTAT‑GCAAg                                                                                                                                             <  2:1027349/137‑1 (MQ=255)

tGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTAT‑GCAATccc                                                                                                                                          >  1:3310339/1‑140 (MQ=255)

tGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTAT‑GCAAGccc                                                                                                                                          >  1:1152424/1‑140 (MQ=255)

tGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTAT‑GCAAGccc                                                                                                                                          >  1:279956/1‑140 (MQ=255)

     tatGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTAT‑GCAAGCCCTTccc                                                                                                                                     >  1:2334863/1‑140 (MQ=255)

     tatGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATAAATATTAGAACGTAT‑TCAATcccatacc                                                                                                                                     >  1:7037/1‑135 (MQ=255)

        ggATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTAT‑GCAAGCCCTTCCCaaa                                                                                                                                  >  2:4911131/1‑140 (MQ=255)

          aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTGTTCTATAAATGAACCAGTCGGTATTACCGGGATCATGATTCGATCGTCT‑GCAAGCGCTTCCCcaaaa                                                                                                                                >  1:839412/1‑140 (MQ=255)

          aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTAT‑GCAAGCCCTTCCCaaaaa                                                                                                                                >  1:3662037/1‑140 (MQ=255)

          aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTAT‑GCAAGCCCTTCCCaaaaa                                                                                                                                >  1:942644/1‑140 (MQ=255)

          aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTAT‑GCAAGCCCTTCCCaaaaa                                                                                                                                >  2:314743/1‑140 (MQ=255)

          aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTAT‑GCAAGCCCTTCCCaaaaa                                                                                                                                >  1:3642170/1‑140 (MQ=255)

          aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTAT‑GCAAGCCCTTCCCaaaaa                                                                                                                                >  1:1666965/1‑140 (MQ=255)

          aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTAT‑GCAAGCCCTTCCCaaaaa                                                                                                                                >  1:2211244/1‑140 (MQ=255)

            tGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTAT‑GCAATCCCATCCCAAAAACg                                                                                                                              >  2:3742092/1‑140 (MQ=255)

            tGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTAT‑GCAAGCCCTTCCCAAAAAcc                                                                                                                              >  1:2628110/1‑139 (MQ=255)

            tGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTAT‑GCAAGCCCTTCCCAAAAACg                                                                                                                              >  1:2977447/1‑140 (MQ=255)

            tGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATAATGATTCGATCGTAT‑GCAAGCCCTTCCCAAAAACg                                                                                                                              >  1:1731542/1‑140 (MQ=255)

                         ctctTTTCGATATTTTTATCACTTTTTAAGCATAACCTTCAATGTACTCGCACAGGTCTTAAATAAATCACCCCGCGGTTATTACCTGTATCATGATGCGATCGTAG‑CCAAGCCCTACCCAACAACTAAAACACGActct                                                                                                                 <  2:945369/140‑1 (MQ=255)

                              ttCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTAT‑GCAAGCCCTTCCCAAAAACGATAAAACGACTCTGctta                                                                                                            >  2:3108688/1‑139 (MQ=255)

                                          aTCACCTTGTAAGAATAACCTTAAAATTACTCGTACAGTTCTTTTATAAATCAACAAGTCTTTATTACGTTTATCATTATTCGATCGTGT‑GAAACCCCTCCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCa                                                                                                <  2:2052816/140‑1 (MQ=255)

                                                ttttAAGCATAACATTCAATGTACGCGGAATGGTTTTCTATAAATGAACCAGTCTTTATAACCTTTATATTGTTTCTCTGGTAT‑GAACGCCCTTCCCAAAACCCAAAACACGACTCTGCTTCTTCAAGTTCGCAAGACAt                                                                                          <  2:2977447/137‑1 (MQ=255)

                                                ttGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTTTATCATGATTCGATCGTAT‑GCAAGCCATTCCCAAAAAAGATAAAAAGACTCTGCTTCTTCAAGTTCGCAAGACAt                                                                                          >  2:2451758/1‑140 (MQ=255)

                                                ttGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTAT‑GCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACAt                                                                                          >  1:3570690/1‑140 (MQ=255)

                                                ttGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTAT‑GCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACAt                                                                                          >  1:1526136/1‑140 (MQ=255)

                                                ttGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTAT‑GCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGATCGCAAGACAt                                                                                          >  2:2536176/1‑140 (MQ=255)

                                                ttGGAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTCT‑GCACTCCCTTCCCAAAAACGATAAATCGACTCTGCTTATTCAAGTGCGCAAGACAt                                                                                          >  1:3705439/1‑140 (MQ=255)

                                                 tGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTAT‑GCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATa                                                                                         >  2:784391/1‑140 (MQ=255)

                                                     aGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTAT‑GCAAGCCCTTCCCAAAAACGATAAAAAGACTCTGCTTCTTCAAGTTCGCAAGACATaacaa                                                                                     >  2:4031431/1‑140 (MQ=255)

                                                         tAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTAT‑GCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtg                                                                                 >  1:4855333/1‑140 (MQ=255)

                                                         tAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTAT‑GCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtg                                                                                 >  1:3870039/1‑140 (MQ=255)

                                                         tAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCAAGATTCGATCGTAT‑GCAAGCCCTTCCCAAAAAAGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACAGAACAACttt                                                                                 >  1:3352005/1‑139 (MQ=255)

                                                            ccTTCAATGTACTCGCCCTGGTTTTCTATAAATGAACCCGTGGTTTTTACCTGTATCTTTTTTCGATCGTAT‑GAACGCCCGCCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtgatg                                                                              <  2:4646396/140‑1 (MQ=255)

                                                            ccTTCAAATTACTCGTACATGTATTCTATAAATGAACCAGTCGTTATTACCTGTATCATGTTTCGATCTTTT‑GCAAGCCCTTCCAAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtgatg                                                                              <  2:3857651/140‑1 (MQ=255)

                                                            ccTTCAAAGTACCCGCACCGTTCTTATATAAATCAACCCGTCGTTATTACCTGTATCTTGATTCGATCGTAT‑GAACGCCCTGCCCAAAAAGGATAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtgatg                                                                              <  2:2181853/140‑1 (MQ=255)

                                                            ccTTAAAAGTACTCGGACAGTTCTTATATAAATCAACCAGTCTTTATTACCTGTATCATGTTTCGATCGTAG‑GCACGCCCTGCCAAAAACCGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtgatg                                                                              <  2:3310339/140‑1 (MQ=255)

                                                            ccTACAAAGTAAGCGGACCTGTGTTATATACATTAACCATTCTTTATTACGTGTATCTTTATTCTTTCGTCT‑CAACCCCCTTCCCAAAAACCATAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtgatg                                                                              <  2:1768098/140‑1 (MQ=255)

                                                            cattaaaaTTACTCGGACTGTTCTTCTATAAATCAACCAGTCTTTATTACCTGTATCATGATTCTAGCGGAT‑GCACCCCCTACCAAAAAACCAAACAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtgatg                                                                              <  2:719188/135‑1 (MQ=255)

                                                            acTTAAAATTACTGGGACTGTTTTTATATAAATCAACCATTCGTTATTACCTTTATATTGATTCTATCTTTT‑GCACGCCCTCCCAAAAAACTATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtgatg                                                                              <  2:1582577/139‑1 (MQ=255)

                                                             cTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTGATTACCTGTATCATGATTCGATCGTAT‑GCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACCTAACAACTtgttga                                                                             >  1:358732/1‑140 (MQ=255)

                                                                 aaaGTACTCGGACAGGTCTTCTATAAATGAACCATTCGTTATTACCTGTATCATTATTCGATCGTAT‑GCAAGCCCTTCCCAAAAACGATAAAACGAAAATGCTTATTCAAGTTCGCAAGACATAACAACTTGATGAGGCt                                                                         >  2:4308850/1‑140 (MQ=255)

                                                                 aaaGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTAT‑GCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAATTTCGCAAGACATAACAACTTGATGAGGCt                                                                         >  2:1201131/1‑140 (MQ=255)

                                                                 aaaGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTAT‑GCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAATACATAAAAACATGATGAgggt                                                                         >  1:1306102/1‑138 (MQ=255)

                                                                 aaaGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTAT‑GCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCt                                                                         >  2:327222/1‑140 (MQ=255)

                                                                 aaaGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTAT‑GCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCt                                                                         >  2:3772594/1‑140 (MQ=255)

                                                                 aaaGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTAT‑GCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACAAAACAACTTGATGAGGCt                                                                         >  1:4413909/1‑140 (MQ=255)

                                                                 aaaGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTAT‑GCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACAAAACAACATGATGAGGCt                                                                         >  2:3879634/1‑140 (MQ=255)

                                                                 aaaGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCAATATTCGATCGTAT‑GCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCt                                                                         >  1:1075050/1‑140 (MQ=255)

                                                                       cTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTAT‑TCAAGCCCTTCCCAAAAACGATAAAACGACTCAGCTTCTTCAATTTCGCAAGACATAACAACTTGATGAGTCTTAatag                                                                   >  2:2335406/1‑140 (MQ=255)

                                                                       cTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTAT‑GCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAATTTCGCAAGAAATAACAACTTGATGATGCTTAagag                                                                   >  2:2931698/1‑140 (MQ=255)

                                                                       cTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTAT‑GCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAatag                                                                   >  1:501090/1‑140 (MQ=255)

                                                                       cTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTAT‑GCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAagag                                                                   >  2:1383732/1‑140 (MQ=255)

                                                                       cTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTAACTGTATGATGATTCGATCGTCT‑GCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCGTCAAGTTCGCCAGACATAACAACTTGATGAGGCTTAagag                                                                   >  2:4521414/1‑140 (MQ=255)

                                                                        tCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTAT‑GCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGc                                                                  >  2:4427628/1‑140 (MQ=255)

                                                                           gACAGGTATTAAATAAATCAACCAGTCTTTATTACGTGTATCATGATTCGCCCGCAC‑GCACGCCCTACCAAAAAACTATAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGAt                                                               <  2:899772/140‑1 (MQ=255)

                                                                           gACAGGTATAATATAAATCAACCAGTCGTTTTTACGTTTATAATGATTCTATCGTAT‑GCAAGCCCTTCCAAAAAACGATAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGAt                                                               <  2:1099865/140‑1 (MQ=255)

                                                                               ggTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTAT‑GCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAAAAACTTGATGAGGCTTAAGAGCGATCaag                                                           >  1:2300986/1‑138 (MQ=255)

                                                                               ggTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTAT‑GCAAGCCCTTCCCAAAAACCATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATccgg                                                           >  2:1258986/1‑137 (MQ=255)

                                                                               ggTCTTCTATAAATGAACCAGTCGTTATTACCTGTATAATGATTCGATCGTAT‑GCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTAGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCagg                                                           >  1:4171002/1‑138 (MQ=255)

                                                                                gTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTAT‑GCAATCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACCTAAAAACTTGATGAGGCTAAAGAGCGATCATGt                                                          >  1:4502798/1‑140 (MQ=255)

                                                                                gTCTTCTATAAATGAACCAGTCGGTATTACCTGTATCATTATTCGATCGTAT‑GCAAGCCCTTCCCCAAAAACAAAAAACGACTCTGCTTCTTCAACTTCCCAATACATAACAACTTTATGATGCTTAACAGCGAACAAGt                                                          >  1:3189551/1‑140 (MQ=255)

                                                                                    tctATAAATGAACCCGTGGTTATTACCTGTATCTTTATTCGTTCGTAT‑GAAACCCCTTCCAAAAAACGATAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTaa                                                      <  2:414654/140‑1 (MQ=255)

                                                                                      tataATTCACCCAGTGTTTATTACGGTTATCATGTTTCGATC‑TATGGCAAGCCCTTCCAAAAAACCAAAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAAt                                                    <  2:3468062/140‑1 (MQ=255)

                                                                                          aaTGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTAT‑GCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGcc                                                >  2:3974105/1‑140 (MQ=255)

                                                                                             caaCCAGTCCTTTTAACCTGTCTCATTATTCGATCGGAT‑CCACGCCCTACCCAAAAACGATAAAACGACTCTGCTTATTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGAt                                             <  1:1980226/139‑1 (MQ=255)

                                                                                              aaCCCGTGGTTATTCCGTTTATCTTTATTCGAGCGTAG‑CCAACCCCTTCCCAAAACCCATAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATa                                            <  1:2679423/140‑1 (MQ=255)

                                                                                              aaCCAGTCTTTTTTACTTTTATAATGATTCGATCTTAT‑GCAAGCCCTGCCAAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATa                                            <  2:2628110/140‑1 (MQ=255)

                                                                                               aCCAGTCGTTATTACCTGTATCATGATTCGATCGTAT‑GCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATAc                                           >  1:573552/1‑140 (MQ=255)

                                                                                                  aGTCGTTATTACCTGTATCATGATTCGATCGTAT‑GCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGc                                        >  1:3927489/1‑140 (MQ=255)

                                                                                                    tCGTTATTACCTGTATCATTATTCGATCGTAT‑TCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAATTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCtt                                      >  1:922222/1‑140 (MQ=255)

                                                                                                               tGTATCATGATTCGATCTTAG‑GCAAGCCCTTCCAAAAAACCACAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTAt                           <  2:4111502/140‑1 (MQ=255)

                                                                                                               tGTATCATGATTCGATCGTAT‑GCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTAt                           <  2:3595879/140‑1 (MQ=255)

                                                                                                                 tATCATGATTCGATCGTAT‑GCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTAttt                         >  1:2761966/1‑140 (MQ=255)

                                                                                                                  aTCATGATTCGATCGTAT‑GCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTc                        >  2:3563225/1‑140 (MQ=255)

                                                                                                                  aTCATGATTCGATCGTAT‑GCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTc                        >  2:4804370/1‑140 (MQ=255)

                                                                                                                      tttttCGATCTTAT‑GAAAGCCATTCCAAAAAACCATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATa                    <  2:332936/137‑1 (MQ=255)

                                                                                                                      tGATTCGATCGTAT‑GCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATa                    >  1:2070423/1‑140 (MQ=255)

                                                                                                                       gATTCGATCGTAT‑GCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATaa                   >  2:3659719/1‑140 (MQ=255)

                                                                                                                       gATTCGATCGTAT‑GCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATaa                   >  2:2741879/1‑140 (MQ=255)

                                                                                                                        aTTCGATCGTAT‑GCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACCTAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATaaa                  >  1:1393665/1‑140 (MQ=255)

                                                                                                                        aTTCGATCGTAT‑GCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATaaa                  >  1:4754467/1‑140 (MQ=255)

                                                                                                                        aTTCGATCGTAT‑GCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATaaa                  >  2:1474141/1‑140 (MQ=255)

                                                                                                                           cGATCGTAT‑GCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATAAAATc               >  2:2705688/1‑140 (MQ=255)

                                                                                                                           cGATCGTAT‑GCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATAAAATc               >  2:2014315/1‑140 (MQ=255)

                                                                                                                           cGATCGTAT‑GCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATAAAATc               >  2:1946109/1‑140 (MQ=255)

                                                                                                                             aTCGTAT‑GCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATAAAATCtt             >  1:4264411/1‑140 (MQ=255)

                                                                                                                             aTCGTAT‑GCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATAAAATCtt             >  2:4713032/1‑140 (MQ=255)

                                                                                                                                 ttatcaaaGCCCTCCCAAAAAACTAAAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATAAAATCTTccc          <  2:1336569/134‑1 (MQ=255)

                                                                                                                                  aT‑GCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATAAAATCTTCCCCt        >  1:2538165/1‑140 (MQ=255)

                                                                                                                                   t‑GCAACCCGTGCCCAACCACGATAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACGTGATGAGGCTTAAGAGCGATCATGTCGAAATTGCCGATACTGCTTATAGCGGTTATTTCCATAAAATCTTCCCCtt       <  2:4695883/140‑1 (MQ=255)

                                                                                                                                    tgaaaGCCGTACCAAAAAACGATAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATAAAATCTTCCCCtt       <  2:80273/137‑1 (MQ=255)

                                                                                                                                    tgaaaGCCCTACCAAAAAACGAAAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATAAAATCTTCCCCtt       <  2:4964388/137‑1 (MQ=255)

                                                                                                                                      aaaGCCCTTCCAAAAAACTATAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATAAAATCTTCCCCTTAt     <  2:657091/139‑1 (MQ=255)

                                                                                                                                        aGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTTATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATAAAATCTTCCCCTTATCa   >  1:752757/1‑140 (MQ=255)

                                                                                                                                         gCCCTTCCAAAAAACTATAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATAAAATCTTCCCCTTATCAg  <  1:4656088/140‑1 (MQ=255)

                                                                                                                                         gCCCTTCCAAAAAACGATAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATAAAATCTTCCCCTTATCAg  <  2:1666965/140‑1 (MQ=255)

TGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTAT‑GCAAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATAAAATCTTCCCCTTATCAG  >  NZ_CP034943/3851118‑3851393

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`