BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	3,851,237	G→T	10.1%	I46I (ATC→ATA)	EO946_RS19820 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	3,851,237	0	G	T	10.1%	428.5 / 5.0	196	I46I (ATC→ATA)	EO946_RS19820	hypothetical protein
Reads supporting (aligned to +/- strand): ref base G (128/38); new base T (8/22); total (136/60)
Fisher's exact test for biased strand distribution p-value = 1.74e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 200 total aligned reads displayed.

CAATGTCCCAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGA‑TAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATAA  >  NZ_CP034943/3851098‑3851376

cAATGTCCCAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATg                                                                                                                                              >  1:2592406/1‑140 (MQ=255)

   tGTCCCAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGAtt                                                                                                                                           <  2:1866452/140‑1 (MQ=255)

     tCCCAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCg                                                                                                                                         >  1:468192/1‑140 (MQ=255)

     tCCCAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCg                                                                                                                                         >  1:903632/1‑140 (MQ=255)

     tCCCAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCg                                                                                                                                         >  2:627742/1‑140 (MQ=255)

     tCCCAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGGCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCg                                                                                                                                         >  1:2422021/1‑140 (MQ=255)

        cactCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTGTCGCTAGTTGTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATACATGAACCAGTCGTTATTACCTGTATCATGATTCGATc                                                                                                                                      <  1:1054640/137‑1 (MQ=255)

        cAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATc                                                                                                                                      <  1:1055702/140‑1 (MQ=255)

        cAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATc                                                                                                                                      <  1:342242/140‑1 (MQ=255)

        cAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATc                                                                                                                                      <  1:991756/140‑1 (MQ=255)

        cAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATc                                                                                                                                      <  1:287593/140‑1 (MQ=255)

        cAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATc                                                                                                                                      <  1:1888309/140‑1 (MQ=255)

        cAATCTGCTCTCTGTAATATGGATTGGAAATGGAAGTCTCTTTTCGCTAGTTTTATCACCTTGTAACCATAACCTTCACAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATc                                                                                                                                      <  1:809985/140‑1 (MQ=255)

          aTCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGt                                                                                                                                    >  2:721340/1‑140 (MQ=255)

          aTCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTGATTACCTGTATAATGATTCGATCGt                                                                                                                                    >  1:2100262/1‑140 (MQ=255)

              gCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGc                                                                                                                                <  2:757620/140‑1 (MQ=255)

               ctctctGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATTATTCGATCGTATGCa                                                                                                                               >  2:1808589/1‑140 (MQ=255)

               ctctctGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCAAGATTCGATCGTATGCa                                                                                                                               >  1:373960/1‑140 (MQ=255)

               ctctctGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTAAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCa                                                                                                                               >  2:1863143/1‑140 (MQ=255)

                   ctGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGcc                                                                                                                           <  1:1890320/140‑1 (MQ=255)

                   ctGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGcc                                                                                                                           <  1:1166637/140‑1 (MQ=255)

                    tGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGccc                                                                                                                          >  2:911906/1‑140 (MQ=255)

                    tGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGccc                                                                                                                          <  2:1223255/140‑1 (MQ=255)

                    tGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTGATTAACTGTATCATGATTCGATCGTATGCAAGccc                                                                                                                          >  1:1194821/1‑140 (MQ=255)

                    tGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGAATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTAATACCAGTATCATGATTCGATCGTATGCAAGccc                                                                                                                          >  2:3088108/1‑140 (MQ=255)

                       aaTATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTAGATAGTATTCAAGCCCTTc                                                                                                                       >  1:1916887/1‑140 (MQ=255)

                            ggATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTGTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCaaa                                                                                                                  <  2:967075/140‑1 (MQ=255)

                              aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGTTCTTCTATAAATGAACCAGTCGTGAATACCTTTATAATGATTCCATCGTATGAAACCCCTTCCCaaaaa                                                                                                                >  1:2918208/1‑140 (MQ=255)

                              aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTTTATCATGATTCGATCGTATGCAAGCCCTTCCCaaaaa                                                                                                                >  1:2057122/1‑140 (MQ=255)

                              aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATTCAAGCCCTTCCCaaaaa                                                                                                                >  2:648245/1‑140 (MQ=255)

                              aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAATCCCTTCCCaaaaa                                                                                                                >  2:867519/1‑140 (MQ=255)

                              aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCaaaaa                                                                                                                >  1:335976/1‑140 (MQ=255)

                              aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCaaaaa                                                                                                                >  1:1179817/1‑140 (MQ=255)

                              aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCaaaaa                                                                                                                >  2:1295176/1‑140 (MQ=255)

                              aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCaaaaa                                                                                                                >  2:2613825/1‑140 (MQ=255)

                              aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTACCaaaaa                                                                                                                >  2:2924854/1‑140 (MQ=255)

                              aTTGGAAATGGAATTCTCTTTTCGCGAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCaaaaa                                                                                                                >  1:2128658/1‑140 (MQ=255)

                               ttGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAAc                                                                                                               >  1:2815594/1‑140 (MQ=255)

                                tGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACg                                                                                                              >  2:2358274/1‑140 (MQ=255)

                                tGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTAACATGAGTCGATCGTATGGAAGCCCTTACCaaaaaag                                                                                                              >  1:1489114/1‑138 (MQ=255)

                                           ttCTCTTTTCGTTATTTTTAACACCTTTTAAGCATAACCTTCAAATTACTCGGACTGGTTTTATATAAATGAACCAGTCTTTATTACCTTTATCATTATTCGATCGTATGCAAGCCCTTCCCAAAACCGA‑TAAAACGAct                                                                                                  <  2:1506280/140‑1 (MQ=255)

                                              tctTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTCCCTGTATCATGATTGGATCGTATGCAAGCCCTTCCCACAAACGA‑TAAAACGACTCTg                                                                                               >  2:1976355/1‑140 (MQ=255)

                                              tctTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGA‑TAAAACGACTCTg                                                                                               >  2:2201692/1‑140 (MQ=255)

                                              tctTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGA‑TAAAACGACTCTg                                                                                               >  2:991829/1‑140 (MQ=255)

                                                ttttCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCTGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATTCAAGCCCTTCCCAAAAACGA‑TAAAACGACTCTGct                                                                                             >  1:1312754/1‑140 (MQ=255)

                                                ttttCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAAAGA‑TAAAACGACTCTGct                                                                                             >  1:2966192/1‑140 (MQ=255)

                                                ttttCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACATGTATCATGAGTCGATCGTATTCAAGCCCTTCCCAAAAACGA‑TAAAAAGACTCTGct                                                                                             >  1:2679053/1‑140 (MQ=255)

                                                ttttCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTAATCGCACAGTTCTTCTATAAATGAACCAGTCGTGCTTACCTGGAGCATGATAGGGTGGTATGCAAGCACTTCCCAAACACGA‑GAATACGACTCTGct                                                                                             >  1:2966571/1‑140 (MQ=255)

                                                     gCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATTATTCGATCGTATGCAACCCCTTCCCAAAAACGA‑TAAAACGACTCTGCttattc                                                                                        >  1:449913/1‑140 (MQ=255)

                                                     gCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAAAGA‑TAAAACGACTCTGCttcttc                                                                                        >  1:2911093/1‑140 (MQ=255)

                                                       ttttttaATCACCTTGTAACCATAACCTGCAAAGTACTCGGACTGTTATTCTAAAAATCAACCAGACGTTTTTACCTGTAGCCTGATTCGATCGGATCCACGCCCTCCCAAAAAACCA‑TAACACGACTCTGCTTCTTCaa                                                                                      <  2:2095953/137‑1 (MQ=255)

                                                          ttttATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATAATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGA‑TAAAACGACTCTGCTTCTTCAAGtt                                                                                   >  1:742431/1‑140 (MQ=255)

                                                            ttttCACGTTTTAACCATAAACTTAAAATAAATCGTACAGTTATTCTATAAATCAACCTGTCTTTATTCCCTGTATCATGATTCTTTCGTATGAAAGCCCTACCAAAAAACCA‑TAAAACGACTCTGCTTCTTCAAGTTCg                                                                                 <  2:2004559/137‑1 (MQ=255)

                                                              aTCACTTTTTAACAATAACTTTCAACGTACTCGTACCGGTCTTCTATAAATGAACCAGTCGTTTTTACCTGTATAATTTTTGGATCGTATGCAAGCCCGTCCCAAAAACGA‑AAAAACGACTCTGCTTCTTCAAGTTCGCa                                                                               <  2:56666/140‑1 (MQ=255)

                                                              aTCACGTTGTAAGAATAACCTTCAAAGTACGCGGACAGGTATTATATAAATGAACCCGTCGTTATTACCTGTTTCATTTTTGGAGCGTATGCACGCCCTCCCCAAAAACGA‑AAACACGACTCTGCTTCTTCAAGTTCGCa                                                                               <  2:2774133/140‑1 (MQ=255)

                                                                aactttttAAGCATAACCTTAAATGTACTCGTACTTTTATTATAAAAATTAACCTGTTTTTTTTACTTTTATTTTTTTTCTATCTTTTGCAAGCCCTACACAAAAACTA‑AAAAACGACTCTGCTTCTTCAAGTTCGCAAg                                                                             <  1:1359884/133‑1 (MQ=255)

                                                                     tGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAATCCCTTCCCAAAAACGA‑TAAAACGACTCTGCTTCTTCAATTTAGCAAGACATa                                                                        >  1:1543601/1‑140 (MQ=255)

                                                                     tGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGA‑TAAAACGACTCTGCTTCTTCAAGTTAGCAAGACATa                                                                        >  1:2752771/1‑140 (MQ=255)

                                                                     tGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGA‑TAAAACGACACTGCTTCTTCAAGTTCGCAAGACATa                                                                        >  1:522051/1‑140 (MQ=255)

                                                                         ccaaaacccTCCAAAGTACTCGGACAGTTCTTATATAAATCCACCCGTGTTTATTACCTGTATCATTATTCCACCGTAGCCAACCCCTACCAAAAAACTA‑AAACACGACTCTGCTTCTTCAAGTTCGCAAGACATaacaa                                                                    <  2:1064307/133‑1 (MQ=255)

                                                                             tacccTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCGTCCCAAAAACGA‑TAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtg                                                                >  2:103065/4‑140 (MQ=255)

                                                                             tAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGA‑TAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtg                                                                >  2:735775/1‑140 (MQ=255)

                                                                             tAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGA‑TAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtg                                                                >  1:1011756/1‑140 (MQ=255)

                                                                             tAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGA‑TAAAACGACTCTGCTTCTTCAAGTTCGCAACACATAACAACGtg                                                                >  1:1131232/1‑140 (MQ=255)

                                                                             tAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGA‑AAAAAACACTCTGCATCTTCAAGTTCGCAAGACATAACAACAtg                                                                >  1:1829463/1‑140 (MQ=255)

                                                                             tAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCGTCCCAAAAAAGA‑TAAAACGACTCTGCTTCTTCAAGTTCGCAACACATAACAACTtg                                                                >  1:857268/1‑140 (MQ=255)

                                                                             tAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGAAAGCCCTTCCCAAAAACGA‑TAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtg                                                                >  2:114387/1‑140 (MQ=255)

                                                                             tAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTGATTACCGGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGA‑TAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtg                                                                >  1:1247180/1‑140 (MQ=255)

                                                                                ccTTAAATGTACGCGGACTGTTATTCTATAAATGACCCAGTGGTTATTACCTTTATATTTATTCGCGCGGTGGAACGCCGTTCCCAAAAACGA‑TACCACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtgatg                                                             <  2:2422021/140‑1 (MQ=255)

                                                                                ccTACACAGTACTCGGACTGGTGTTATATAAATCAACACGTCGTATTTCCCTGTATCTTTCTTCTATCGTCTGACAGCCCTTCCCAAACACTA‑TAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtgatg                                                             <  2:258238/140‑1 (MQ=255)

                                                                                     aaaGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGA‑TAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCt                                                        >  1:1685204/1‑140 (MQ=255)

                                                                                           ctgggACAGGTTTTTTATAAATCAACCAGTCGTTATTACCTTTATCATTATTCCATCGTATGAAACCCCTTCCAAAAAACTA‑TAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAagag                                                  <  2:2124660/137‑1 (MQ=255)

                                                                                           cTCGGACTGGTATTATAAAAATCAACCAGTCTTTATTACCTGTATCATGTTTCGATCGTAGCAAAGCCCTCCCCAAAAACCA‑AAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAagag                                                  <  1:2217459/140‑1 (MQ=255)

                                                                                           cTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGA‑TAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAagag                                                  >  1:3061506/1‑140 (MQ=255)

                                                                                               tactgtacttctATAAATCACCCTTTCGTTATTCCCTGTATATTTATTCGATCTTATGAAAGCCCTTCCAAAAAACTA‑TAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGAt                                              <  2:335976/133‑1 (MQ=255)

                                                                                                   ggTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGA‑TAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAATAGCGATCATg                                          >  1:551765/1‑140 (MQ=255)

                                                                                                   ggTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGA‑TAAAAAGACTCTGCTTCTTCAAGTTCGCAAGACATAAAAACTTGATGAGGCTTAAGAGctgtctct                                          >  1:2638203/1‑133 (MQ=255)

                                                                                                        tctATAAATTAACGATTTTTATTTACCTGTATCTTGATTCGATCGTATGCACCCCCTTCCAAAAAACTA‑TAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTaa                                     <  1:1728067/140‑1 (MQ=255)

                                                                                                        tcaaaaaaTCAACCAGTCGTTATTACCTGTATCATGATTCGATCGTTTGAACGCCC‑TCCCAAAAACCATTAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTaa                                     <  2:221167/135‑1 (MQ=255)

                                                                                                          tataAATGAACCAGTCGTTCTTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGA‑TAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAAt                                   >  2:388270/1‑140 (MQ=255)

                                                                                                          tataAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGA‑TAAAACGACTCTTCTTCTTAAAGTTAGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAAt                                   >  2:1597422/1‑140 (MQ=255)

                                                                                                             aaaTCAACCAGTCGTTTTTAACTTTATCATGATTCGATCGTATGAAAGCCCTTCCAAAAAACTA‑TAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGc                                <  2:596232/140‑1 (MQ=255)

                                                                                                                 cacacAGTCGTTTTTACGTTTATCATTATTCGATCTTATGCAAGCCCTTCCAAAAAACCA‑TAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGAt                            <  2:1751754/136‑1 (MQ=255)

                                                                                                                 caaCCAGTCGTTATTACATGTATCATGATGCGATCGTATGCAAGCCCTCCCAAAAAACGA‑TAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGAt                            <  1:1767081/139‑1 (MQ=255)

                                                                                                                  acccATTCTTTATTACCTGTAAAATGATTCTATCCTATGCAAGCCCTACCCAAAAACCA‑AAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATa                           <  2:1416040/138‑1 (MQ=255)

                                                                                                                  acccAGTATTTATTACCTTTAACATTATCCGAGCTTGTCAAAGCACTCCCAAAAAACCA‑TACCACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATa                           <  1:2315530/138‑1 (MQ=255)

                                                                                                                  aacacgTCGTTATTACCTTTATCATGATCCGACCGTATGCAACCCCTTCCAAAAAACTA‑AAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATa                           <  2:454279/135‑1 (MQ=255)

                                                                                                                  aaCCTGTCTTTATAACGTGTATAATGATTCTATCTTATGCAAGCCCTACCAAAAAACGA‑TAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATa                           <  1:3089914/140‑1 (MQ=255)

                                                                                                                  aaCCAGTCTTTTTTACCTTTATAATGATTCGAGGGTATGAAAGCCCTTCCAAAAAACTA‑TAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATa                           <  2:3068782/140‑1 (MQ=255)

                                                                                                                  aaCCAGTCGTTATTACCTGTATCATTATTCGATCGTATGCAAGCCCTTCCCAAAAACGA‑TAAAACCACTGTGCTTCTTCAAGTTAGAAAGACATAAAAACTTGATGAGGCTTAAGTGCGATAATGTCTAAATTGCCGATa                           >  2:1201826/1‑140 (MQ=255)

                                                                                                                   aCCAGTCGTTATTACCTGTATCATGATTCGGTCGTATGCAAGCCCTTCCCAAAAACGA‑TAAAACGAATCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCAAGTCTAAATTGCCGATAc                          >  1:315150/1‑140 (MQ=255)

                                                                                                                   aCCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGA‑TAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATTTCTAAATTGCCGATAc                          >  1:261323/1‑140 (MQ=255)

                                                                                                                      aGTCGTTATTACCGGTATCATTATTCGATCGTATGCAAGCCCTTCCCAAAAACGA‑TAAAACGACTCTGCTTCTTCAAGTTCGCAAGTCATAACAACTTGATGAGGCTTAAGAGCGATCATTTCTTAATTGCCGATACTGc                       >  1:1506344/1‑140 (MQ=255)

                                                                                                                        tCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGA‑TAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGATCGGTCATGTCTAAATTGCCGATACTGCtt                     >  1:655503/1‑140 (MQ=255)

                                                                                                                                  cTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGA‑TAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTa           >  1:197958/1‑140 (MQ=255)

                                                                                                                                   tGTATAATTTTTCGATCTTATTCAAGCCCGGCCAAAAAACTA‑TAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTAt          <  2:864619/140‑1 (MQ=255)

                                                                                                                                    gaaTCATTATTCCTTCTTATCCAAGCCAGTCCCTAAACCGA‑TAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTAtt         <  2:2608460/138‑1 (MQ=255)

                                                                                                                                     tATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGA‑TAAAACGACTCTGCTTCTTCAATTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGATTATAGCGGTTAtgt        >  1:678321/1‑138 (MQ=255)

                                                                                                                                     tATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGA‑TAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTAttt        >  2:2651055/1‑140 (MQ=255)

                                                                                                                                     tATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGA‑TAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTAttt        >  2:2551325/1‑140 (MQ=255)

CAATGTCCCAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAACGA‑TAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATAA  >  NZ_CP034943/3851098‑3851376

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`