BRESEQ :: Evidence

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	2,991,227	0	T	G	30.7%	242.6 / 42.9	163	intergenic (+28/‑179)	gbsR/bslA	transcriptional regulator GbsR/biofilm‑surface layer protein BslA
Reads supporting (aligned to +/- strand): ref base T (31/82); new base G (50/0); total (81/82)
Fisher's exact test for biased strand distribution p-value = 7.85e-21
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 167 total aligned reads displayed.

ATACGAGCAACTGGAAAGCTCTAAAGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTG‑CAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAA‑AACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATT‑ACT‑ACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCGGG  >  NZ_CP034943/2991091‑2991363

ataCGAGCAACTGGAAAGCTCTAAAGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTG‑CAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAA‑AACGCCTGGGAAACCGGGCGtgttt                                                                                                                                         >  1:2250350/1‑140 (MQ=255)

     aGCAACTGGAAAGCTCTAAAGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTG‑CAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAA‑AACGCCTGGGAAACCGGGCGTGtttttttt                                                                                                                                    >  1:1957863/1‑140 (MQ=255)

     aGCAACTGGAAAGCTCTAAAGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTG‑CAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAA‑AACGCCTGGGAAACCGGGCGTGTTTTTGtt                                                                                                                                    >  1:2434582/1‑140 (MQ=255)

     aGCAACTGGAAAGCTCTAAAGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTG‑CAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAA‑AACGCCTGGGAAACCGGGCGTGTTTTTGtt                                                                                                                                    >  2:2447216/1‑140 (MQ=255)

      gCAACTGGAAAGCTCTAAAGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTG‑CAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAA‑AACGCCTGGGAAACCGGGCGTGTTTTTGttg                                                                                                                                   >  1:3041133/1‑139 (MQ=255)

        aaCTGGAAAGCTCTAAAGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTG‑CAGAGCGGGGAAATTTTTAAATTTATAACGATTGAAACAAAGTAAAACAAA‑AACGCCTGGGAAACCGGGCGTTGTTTTggtttt                                                                                                                                 >  1:771050/1‑135 (MQ=255)

          cTGTAAACCTCTAAAGCTTAATATTATGGGCTGAAACGGCTTGCTTAATCGGTG‑CCCAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAACGTAAACCAAA‑ACCGCCTGGGAAACCGGGCGTTTTTTTGTTCtttt                                                                                                                               <  1:1647471/140‑1 (MQ=255)

          cTGGAAAGCTCTAAAGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTG‑CAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAA‑AACGCCTGGGAAACCGGGCGTGTtttttttttttt                                                                                                                               >  2:2753198/1‑140 (MQ=255)

          cTGCAAAGCTCTAAAGCTTACTATGATTGCCTCAAACGGCTTGCTCACACGGCG‑CAGAGCGGGGAAATTTTTAAATTTATACCGATTCAAACAAAGAAAAAAAAA‑CACGCCTGGGAAACCGGGCGATTTTTTGTTCtttt                                                                                                                               <  1:536240/140‑1 (MQ=255)

          cTGCAAAGCAATAAAGCTTACTATTTTTGGCTGAACCGGCTGGCTGAATCGGTG‑CCTCGCGGGGAATTTTTAAACTTTATACCGATTGAAACAACGTAACACAAA‑AACGCCTGGGAAACCGGGCGTTTTTTTGTTCtttt                                                                                                                               <  1:3393980/140‑1 (MQ=255)

                  ctctAAAGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTG‑CAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAA‑AACGCCTGGGAAACCGGGCGTTTTTTTTTTCTTTTTTagttac                                                                                                                       >  2:203245/1‑135 (MQ=255)

                  ctctAAAGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTG‑CAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAA‑AACGCCTGGGAAACCGGGCGTGTTTTTGTTGttttttttttta                                                                                                                       >  1:2002022/1‑134 (MQ=255)

                  ctctAAAGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTG‑CAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAA‑AACGCCTGGGAAACCGGGCGTGTTTTTGTTGTTTTTTATGTaa                                                                                                                       >  2:2501978/1‑140 (MQ=255)

                  ctctAAAGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTG‑CAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAA‑AACGCCTGGGAAACCGGGCGTGTTTTTGTTCTTTTTTATGTaa                                                                                                                       >  2:689399/1‑140 (MQ=255)

                   tctAAAGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTG‑CAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAA‑AACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAAt                                                                                                                      <  2:1073364/140‑1 (MQ=255)

                   tctAAAGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTG‑CAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAA‑AACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAAt                                                                                                                      <  2:1326930/140‑1 (MQ=255)

                     tAAAGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTG‑CAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAA‑AACGCCTGGGAAACCGGGCGTTTTTTTTTTGTTTTTTGTGttatt‑a                                                                                                                   >  1:776930/1‑140 (MQ=255)

                     tAAAGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTG‑CAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAA‑AACGCCTGGGAAACCGGGCGTGTTTTTGTTATTTTTTATGttatt‑a                                                                                                                   >  1:2239407/1‑140 (MQ=255)

                            tACTATGATTGGCTGAAACGGCTTGCTGAATCGGTG‑CAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAA‑AACGCCTGGGAAACCGGGCGTTTTTTTTTTGTTTTTTTTTTAAAT‑AAT‑ACaaa                                                                                                           >  2:508713/1‑140 (MQ=255)

                            tACTATGATTGGCTGAAACGGCTTGCTGAATCGGTG‑CAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAA‑AACGCCTGGGAAACCGGGCGTTTTTTTTTTGTTTTTTATGTAATT‑AAT‑Aaaaa                                                                                                           >  2:361701/1‑140 (MQ=255)

                            tACTATGATTGGCTGAAACGGCTTGCTGAATCGGTG‑CAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAA‑AACGCCTGGGAAACCGGGCGTTGTTTTGTTGTGTTTTATGTACTT‑ACT‑ACaaa                                                                                                           >  2:2807232/1‑140 (MQ=255)

                            tACTATGATTGGCTGAAACGGCTTGCTGAATCGGTG‑CAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAA‑AACGCCTGGGAAACCGGGCGTGTTTTTTTTGTTTTTTTTTTTATT‑AAA‑ACaaa                                                                                                           >  1:1006945/1‑140 (MQ=255)

                            tACTATGATTGGCTGAAACGGCTTGCTGAATCGGTG‑CAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAA‑AACGCCTGGGAAACCGGGCGTGTTTTTGTTTTTTTTTATTTAATt‑aaaaaaaa                                                                                                            >  1:439524/1‑133 (MQ=255)

                             aCTATGATTGGCTGAAACGGCTTGCTGAATCGGTG‑CAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAA‑AACGCCTGGGAAACCGGGCGTTTTTTTTTTGTTTTTTATTTTATT‑AAT‑AAAAAt                                                                                                          >  2:10587/1‑140 (MQ=255)

                             aCTATGATTGGCTGAAACGGCTTGCTGAATCGGTG‑CAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAA‑AACGCCTGGGAAACCGGGCGTTTTTTTGTTGTTTTTTATGTAATT‑ACT‑ACaaaa                                                                                                          >  1:2529535/1‑139 (MQ=255)

                             aCTATGATTGGCTGAAACGGCTTGCTGAATCGGTG‑CAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAA‑AACGCCTGGGAAACCGGGCGTGTTTTTTTTGTTTTTTTTGTTATT‑AAT‑Aaaaaa                                                                                                          >  1:3395718/1‑139 (MQ=255)

                             aCTATGATTGGCTGAAACGGCTTGCTGAATCGGTG‑CAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAA‑AACGCCTGGGAAACCGGGCGTGTTTTTGTTCTTTTTTATGTAATT‑ACT‑AAAAAt                                                                                                          >  2:2893778/1‑140 (MQ=255)

                                  taTTGGCTGAAACGGCTTGCTG‑ATGGGTGCCAGAGCGGTTAAATTTTTAAATTTATACCGATTGAACAAAAGTAAAAAAAA‑CACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATT‑ACT‑ACAAATTTGaa                                                                                                     <  1:2753198/139‑1 (MQ=255)

                                   aTTGGCTGAAACGGCTTGCTGAATCGGTG‑CAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAA‑AACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATT‑ACA‑AGaaaaattaaa                                                                                                    >  2:3274895/1‑133 (MQ=255)

                                   aTTGGCTGAAACGGCTTGCTGAATCGGTG‑CAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAA‑AACGCCTGGGAAACCGGGCGTGTTGTTGTTTTTTTTTTTTTAATT‑AAT‑ACaaaaatttaa                                                                                                    >  1:1200567/1‑133 (MQ=255)

                                    ttGGCTGAAACGGCTTGCTGAATCGGTG‑CAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAA‑AACGCCTGGGAAACCGGGCGTTTTTTTGTTGTTTTTTATGTAAAT‑AAT‑AAAAAATTTaaaa                                                                                                   >  2:446841/1‑140 (MQ=255)

                                    ttGGCTGAAACGGCTTGCTGAATCGGTG‑CAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAA‑AACGCCTGGGAAACCGGGCGTGTTTTTGTTATTTTTTTTTTAATT‑TAT‑ACAAAATTGaaaa                                                                                                   >  2:2174262/1‑140 (MQ=255)

                                       gCTGAAACGGCTTGCTGAATCGGTG‑CAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAA‑AACGCCTGGGAAACCGGGCGTTTTTTTGTTGTTTTTTATTTAATT‑AAT‑AACAATTTGAAAAATa                                                                                                >  2:2983850/1‑140 (MQ=255)

                                       gCTGAAACGGCTTGCTGAATCGGTG‑CAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAA‑AACGCCTGGGAAACCGGGCGTGTTTTTGTTGTTTTTTATGTAATT‑ACA‑ACAAAAATTaaaaaaa                                                                                                >  2:497499/1‑138 (MQ=255)

                                       gCTGAAACGGCTTGCTGAATCGGTG‑CAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAA‑AACGCCTGGGAAACCGGGCGTGTTTTTGTTCTTTTTTTTGTAATT‑AAT‑ACAAATTTTAAAAATa                                                                                                >  2:1171120/1‑140 (MQ=255)

                                         tGAAACGGCTTGCTGAATCGGTG‑CAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAA‑AACGCCTGGGAAACCGGGCGTTTTTTTGTTGTTTTTTATGTAATT‑ACT‑ACAAATTTTAAAAATACg                                                                                              >  2:2641599/1‑140 (MQ=255)

                                           aaaCGGCTTGCTGAATCGGTG‑CAGAGCGGGGAAATTTTTAAATTTATACCGAGTGAAACAAAGTAAAACAAA‑AACGCCTGGGAAACCGGGCGTGTTTTTGTTCTTTTTTATGTAAAT‑AGT‑AAAAAATTGAAAAATAAggg                                                                                            >  2:1512209/1‑140 (MQ=255)

                                             aCGGCTTGCTGAATCGGTG‑CAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAA‑AACGCCTGGGAAACCGGGCGTGTTTTTGTTGTTTTTTATGTAAAT‑ACT‑ACAAATTTTGAAAATACGGGaa                                                                                          >  2:1784573/1‑140 (MQ=255)

                                                 cTTGCTGAATCGGTG‑CAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAA‑AACGCCTGGGAAACCGGGCGTTTTTTTTTTGTTTTTTATTTAATT‑ACT‑AAAAAATTTAAAAATAAGGGaaaaaa                                                                                      >  2:1304103/1‑139 (MQ=255)

                                                   tGCTGAATCGGTG‑CAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAA‑AACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATT‑ACT‑ACAAATTTGAAAAATACGGGAAAAACAt                                                                                    <  2:1013521/140‑1 (MQ=255)

                                                         atgggTG‑CAGCGCGGGTAAATTTTTAAATTTATACCGATTTAAAAAAAGTAAAAAAAA‑AACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATT‑ACT‑ACAAATTTGAAAAATACGGGAAAAACATCCCAtt                                                                              <  1:123290/137‑1 (MQ=255)

                                                         atcttgg‑CAGATCTGGTAAATTTTTAAATTTATACCTATTGAAACAAATTAAAACAAA‑AACGCCTGGGAAACCGGGCTTTTTTTTGTTCTTTTTTATGTAATT‑ACT‑ACAAATTTGAAAAATACGGGAAAAACATCCCAtt                                                                              <  2:1406234/134‑1 (MQ=255)

                                                          tCGGTG‑CAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAA‑AACGCCTGGGAAACCGGGCGTGTTTTTGTTGGTTTTTATTTAAAT‑AAT‑ACAAATTTGAAAAATTCGGGAAAAAAATCCCAttt                                                                             >  2:458499/1‑140 (MQ=255)

                                                             gTG‑CAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAA‑AACGCCTGGGAAACCGGGCGTTTTTTTGTTGTTTTTTATGTTATT‑AAT‑ACAAATTTTTAAAATTCGGGAAAAAACTCCCAttttat                                                                          >  2:3260119/1‑137 (MQ=255)

                                                             gTG‑CAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAA‑AACGCCTGGGAAACCGGGCGTGTTTTTGTTGTTTTTTATGTAATT‑ACT‑ACACATTTTAAAAATAAGGGAAAAACATACCCGTTACt                                                                          >  2:2859944/1‑140 (MQ=255)

                                                             gTG‑CAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAA‑AACGCCTGGGAAACCGGGCGTGTTTTTGGTGTTTTTTTTTTAATT‑AGT‑ACAAATTTGTAAATTACTGGTAAAACATCCCCttttat                                                                          >  2:1703590/1‑137 (MQ=255)

                                                                 cAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAA‑AACGCCTGGGAAACCGGGCGTGTTTTTGTTCTTTTTTATTTAATT‑ACT‑ACAAAATTTTAAAATAAGGGAAAAAAATCACAATTACttac                                                                       >  2:2659325/1‑138 (MQ=255)

                                                                  agagCGGGGAAATTTTTAAATTCATACCGATTGAAACAAAGTAAAACAAA‑CACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATT‑ACT‑ACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCa                                                                      <  1:1339769/140‑1 (MQ=255)

                                                                        gggAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAA‑AACGCCTGGGAAACCGGGCGTGTTGTTGTTGTTTTTTATGTAATT‑AAT‑ACAAATTTGTAAAATACGGGAAAAACATCACAATTAATTAAAAAatat                                                                >  2:1892256/1‑140 (MQ=255)

                                                                         ggAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAA‑AACGCCTGGGAAACCGGGCGTGTTTTTGTTTTTTTTTTTTTTATTAAAA‑AAAAATTTTAAAAAAAGGGGAAAAA‑ATACCAGTTAATTCCaaaaaagt                                                               >  1:1611009/1‑136 (MQ=255)

                                                                         ggAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAA‑AACGCCTGGGAAACCGGGCGTGTTTTTGTTGTTTTTTTTGTTATT‑AATCAAAAATTTTAAAAAAAC‑GGACAAAAATACCATTTAATTGCAAAAGAtt                                                               >  1:213745/1‑140 (MQ=255)

                                                                         ggAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAA‑AACGCCTGGGAAACCGGGCGTGTTTTTGTTGTTTTTTATGTAATT‑ACT‑AAAAAATTTAAAAAAACGGGAAAAACATACCATTTACTTACAAAATAtt                                                               >  2:1619891/1‑140 (MQ=255)

                                                                         ggAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAA‑AACGCCTGGGAAACCGGGCGTGTTTTTGTTCTTTTTTATGTAATT‑AGT‑ACAAATTTGATAAATACGGGAAAAACTTCACAGTTTGTTCCAAACTAtt                                                               >  2:3314807/1‑140 (MQ=255)

                                                                         ggAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAA‑AACGCCTGGGAAACCGGGCGTGTTGTTGGTGTTTTTTTTTTTATT‑AAT‑TCAAAATTTTAAAATAAGGGCAAAAAATCCCAATTTCATAGAAAATAtt                                                               >  1:3004476/1‑140 (MQ=255)

                                                                         ggAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAA‑AACGCCTGGGAAACCGGGCGTGGTTTTGTTGTTTTTTAAGTAATT‑AAT‑ACAAAATTTAAAAAAAATGGAAAAACATACCATTTAATTCCAAAATAtt                                                               >  2:2248718/1‑140 (MQ=255)

                                                                               ttttAAATTTATACCGATTGAAACAATGTAAAACAAA‑CCCGGCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATT‑ACT‑TCAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATgg                                                         <  2:2685522/140‑1 (MQ=255)

                                                                               ttttAAATTTATACCGATTGAAAAAAAGTAAAACAAA‑AACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATT‑ACT‑ACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATgg                                                         <  1:2435365/140‑1 (MQ=255)

                                                                               ttttAAATGTAGCCCGATTGAAACAACGTAAAACAAA‑AACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATT‑ACT‑ACAAAATTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATgg                                                         <  2:1348795/140‑1 (MQ=255)

                                                                                        tataCCGATTGAAACAAAGTAAAACAAA‑AACGCCTGGGAAACCGGGCGTTTTTTTGTTGTTTTTTATGTTATT‑AAT‑AAAAAATTGAAAAAAAAGGGCAAAACATTCCCTTTAGATACAAAAAATTTTATTGTAAtttttt                                                >  2:573079/1‑138 (MQ=255)

                                                                                         ataCCTATTTACACAAA‑CAACACAAACAACGCCTGGGAAACCGCGCGTTTATTTGTTCTTTTTTATGTCATT‑ACC‑ACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTGGATGGTAATTTTATg                                               <  1:446841/140‑1 (MQ=255)

                                                                                         aaaCCGATTCAAACAAACTAAAACAAA‑CCCGCCAGGGAAACCGGGCGTTTTTTTTTTCTTTTTTATGTAATT‑ACT‑ACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATGTGATGGTAATTTTATg                                               <  1:43194/138‑1 (MQ=255)

                                                                                              gATTTAAACAACGTAAAACAAA‑CACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATT‑ACT‑ACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGt                                          <  1:3101051/140‑1 (MQ=255)

                                                                                              gATTGAAAAAAAGTAAAACAAA‑AACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATT‑ACT‑ACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGt                                          <  1:2659325/140‑1 (MQ=255)

                                                                                               aTTGAAACAAAGTAAAACAAA‑AACGCCTGGGAAACCGGGCGTTTTTTTTTTTTTTTTTTTGTAATT‑AAA‑AAAAAATTTTAAAATAAGGGAAAAAAAAACCCATTAATTGCAAAAAATTTTATGGTAATTTTATGTAgtga                                         >  2:933301/1‑137 (MQ=255)

                                                                                               aTTGAAACAAAGTAAAACAAA‑AACGCCTGGGAAACCGGGCGTTTTTTTGTTGTTTTTTATGTAATT‑AAT‑AAAAATTTTAAAAATAAGGGAAAAACATACCATTTACTTCCAAAAGTTTTGTTTGTAATTTTATGTAGGtt                                         >  2:2730686/1‑139 (MQ=255)

                                                                                               aTTGAAACAAAGTAAAACAAA‑AACGCCTGGGAAACCGGGCGTGTTTTTGTTGTTTTTTATGTTATT‑ACT‑ACAAAATTTAAAAATAAGGGAAAAAAATCCGATTTAATTACAAAATAATTTATTGTAATTTTTTTTGGGTa                                         >  2:1802371/1‑140 (MQ=255)

                                                                                               aTTGAAACAAAGAAAAACAAA‑AACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATT‑ACT‑ACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTa                                         <  1:508713/140‑1 (MQ=255)

                                                                                                   aaacaaacAAAAACAAC‑AACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATT‑ACT‑ACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAAt                                     <  1:1758167/140‑1 (MQ=255)

                                                                                                   aaaCAAAGTAAAACAAA‑AACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATT‑ACT‑ACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAAt                                     <  2:122849/140‑1 (MQ=255)

                                                                                                   aaaCAAAGAAAAACAAA‑CACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATT‑ACT‑ACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAAt                                     <  1:905738/140‑1 (MQ=255)

                                                                                                             aaccAAA‑AACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATT‑ACG‑ACAAATTTGAAAAATACGGGAAAAACATCCCATTTACGTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                           <  1:2682348/137‑1 (MQ=255)

                                                                                                             aaacaaa‑cCCGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATT‑ACT‑ACAAATGTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                           <  2:221491/140‑1 (MQ=255)

                                                                                                             aaacaaa‑cACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATT‑ACT‑ACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                           <  1:3166129/140‑1 (MQ=255)

                                                                                                             aaacaaa‑cACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATT‑ACT‑ACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                           <  2:552292/140‑1 (MQ=255)

                                                                                                             aaaaaaa‑aACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATT‑ACT‑ACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAACTATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                           <  1:1306122/140‑1 (MQ=255)

                                                                                                             aaaCACA‑ACCGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAAGT‑ACT‑ACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                           <  2:2100770/140‑1 (MQ=255)

                                                                                                             aaaCACA‑AACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATT‑ACT‑ACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                           <  1:1742119/140‑1 (MQ=255)

                                                                                                             aaaCAAA‑ACCGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATT‑ACT‑ACAAATTTTAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                           <  1:497499/140‑1 (MQ=255)

                                                                                                             aaaCAAA‑AACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATTTAATT‑ACT‑ACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                           <  2:377883/140‑1 (MQ=255)

                                                                                                             aaaCAAA‑AACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATT‑ACT‑ACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                           <  1:1445728/140‑1 (MQ=255)

                                                                                                             aaaCAAA‑AACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATT‑ACT‑ACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                           <  2:669826/140‑1 (MQ=255)

                                                                                                             aaaCAAA‑AACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATT‑ACT‑ACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                           <  2:754355/140‑1 (MQ=255)

                                                                                                             aaaCAAA‑AACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATT‑ACT‑ACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                           <  2:2250350/140‑1 (MQ=255)

                                                                                                             aaaCAAA‑AACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATT‑ACT‑ACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                           <  1:10587/140‑1 (MQ=255)

                                                                                                             aaaCAAA‑AACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATT‑ACT‑ACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                           <  2:2346507/140‑1 (MQ=255)

                                                                                                             aaaCAAA‑AACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATT‑ACG‑ACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                           <  2:2289731/140‑1 (MQ=255)

                                                                                                              aaCAAA‑AACGCCTGGGAAACCGGGCGTGTTTTTGTTGTTTTTTTTGTAAAT‑AAT‑ACAAATTTTAAAAATACGGGAAAAAAAAACCAATTAAGTGCAAAATATTTTATTGTAATTTTATGTTGGTAAAATTTGTttttttg                          >  2:1742119/1‑137 (MQ=255)

                                                                                                              aaCAAA‑AACGCCTGGGAAACCGGGCGTGTTTTTGGTGTTTTTTATGTAATT‑ACT‑AAAAAATTGAAAAATACTGGAAAAACCTAGCATTTAGGTACACAAGATTTGATTGTAAATTTATGTTGGTATAATTTGGTTTTGGc                          >  2:3149479/1‑140 (MQ=255)

                                                                                                                   aacaCGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATT‑ACT‑ACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGtt                      <  1:2239529/137‑1 (MQ=255)

                                                                                                                   aacaCGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATT‑ACT‑ACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGtt                      <  1:665243/137‑1 (MQ=255)

                                                                                                                      aCGCCTGGGAAACCGGGCGTTTTTTTTTTCTTTTTTATGTAATT‑ACT‑ACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCag                   <  2:776930/140‑1 (MQ=255)

                                                                                                                      aCGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATT‑ACT‑ACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCag                   <  2:853036/140‑1 (MQ=255)

                                                                                                                      aCGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATT‑ACT‑ACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCag                   <  2:887961/140‑1 (MQ=255)

                                                                                                                        gCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATT‑ACT‑ACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCagag                 <  2:4062/140‑1 (MQ=255)

                                                                                                                        gCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATT‑ACT‑ACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCagag                 <  2:3004476/140‑1 (MQ=255)

                                                                                                                        gCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATT‑ACT‑ACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCagag                 <  2:1510448/140‑1 (MQ=255)

                                                                                                                             ggAAACCGGGCTTTTTTTTGTTCTTTTTTATGTAATT‑ACT‑ACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGt            <  1:2495026/140‑1 (MQ=255)

                                                                                                                             ggAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATT‑ACT‑ACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGt            <  1:1279390/140‑1 (MQ=255)

                                                                                                                                       cGTTTTTTTGTTCTTTTTTCTGTAATG‑ACT‑ACAAATTTCAAAAATACGGGAAAAACATGCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCGGTTCAGAGGAGGTGAAAAGCggg  <  1:2461434/140‑1 (MQ=255)

                                                                                                                                       cGTTTTTTTGTTCTTTTTTATGTAATT‑ACT‑ACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCggg  <  2:2965009/140‑1 (MQ=255)

ATACGAGCAACTGGAAAGCTCTAAAGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTG‑CAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAA‑AACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATT‑ACT‑ACAAATTTGAAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCGGG  >  NZ_CP034943/2991091‑2991363

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`