BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	1,488,158	G→T	9.6%	D567Y (GAT→TAT)	ade →	adenine deaminase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	1,488,158	0	G	T	9.6%	373.2 / 8.2	168	D567Y (GAT→TAT)	ade	adenine deaminase
Reads supporting (aligned to +/- strand): ref base G (47/99); new base T (19/3); total (66/102)
Fisher's exact test for biased strand distribution p-value = 1.67e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 170 total aligned reads displayed.

GTGAATCAAAGCTTACTGACGCTGCATGATAAATTGTTATTAGTCGGTTTCACAGGCGGGTTTAATCCATTTTTGACGCTGTCGTTTTTAGCTTTGCCTGTCATTCCTGAC‑ATCAAAATGACGACAACAGGATTATTTGATGTAAAATCATTTCAGCAT‑ATTGCATTGCAATAAAAAAAGATGCACTCCAAAGTGAGTGCATC‑TTTTTTTTAAACTTCCATGATGATCGGAAGGATCATCGGACGGCGTTTCGTTTTTTCATATAAGAACGGCGCAAG  >  NZ_CP034943/1488020‑1488297

   aaTCAAAGCTTACTGACGCTGCATGATAAATTGTTATTAGTCGGTTTCACTGGCGGGTTTAATCCATTTTTGACGCTGTCGTTTTTAGCTTTGCCTGTCATTCCTGAC‑ATCAAAATGACGACAACAGGATTATTTGATGt                                                                                                                                           <  2:4334632/140‑1 (MQ=255)

   aaTCAAAGCTTACTGACGCTGCATGATAAATTGTTATTAGTCGGTTTCACAGGCGGGTTTAATCCATTTTTGACGCTGTCGTTTTTAGCTTTGCCTGTCATTCCTGAC‑ATCAAAATGACGACAACAGGATTATTTGATGt                                                                                                                                           <  2:1775271/140‑1 (MQ=255)

   aaTCAAAGCTTACTGACGCTGCATGATAAATTGTTATTAGTCGGTTTCACAGGCGGGTTTAATCCATTTTTGACGCTGTCGTTTTTAGCTTTGCCTGTCATTCCTGAC‑ATCAAAATGACGACAACAGGATTATTTGATGt                                                                                                                                           <  2:3277709/140‑1 (MQ=255)

   aaTCAAAGCTTACTGACGCTGCATGATAAATTGTTATTAGTCGGTTTCACAGGCGGGTTTAATCCATTTTTGACGCTGTCGTTTTTAGCTTTGCCTGTCATTCCTGAC‑ATCAAAATGACGACAACAGGATTATTTGATGt                                                                                                                                           <  2:1022412/140‑1 (MQ=255)

   aaTCAAAGCTTAATGACGCTGCATGATAAATTGTTATTAGTCGGTTTCACAGGCGTGATTAATCCATTTTTGACGCTGTCGTTTTTAGCTTTGCCTGTCATTCCTGAC‑ATCAAAATGACGACAACAGGATTATTTGATGt                                                                                                                                           <  2:4258646/140‑1 (MQ=255)

        aaGCTTACTGACGCTGCATGATAAATTGTTATTAGTCGGTTTCACAGGCGGGTTTAATGCATTTTTGACGCTGTCGTTTTTCGCTTTGGCTGGCATTCGGGTC‑ATCAAAATGACGTCAACAGGATTATTTGCTGTaaaag                                                                                                                                      >  2:3817275/1‑139 (MQ=255)

        aaGCTTACTGACGCTGCATGATAAATTGTTATTAGTCGGTTTCACAGGCGGGTTTAATCCATTTTTGACGCTGTCGTTTTTAGCTTTGCCTGTCATTCCTGAC‑ATCAAAATGACGACAACAGGATTATTTGATGTAAAAt                                                                                                                                      <  2:171772/140‑1 (MQ=255)

        aaGCTTACTGACGCTGCATGATAAATTGTTATTAGTCGGTTTCACAGGCGGGTTTAATCCATTTTTGACGCTGTCGTTTTTAGCTTTGCCTGTCATTCCTGAC‑ATCAAAATGACGACAACAGGATTATTTGATGTAAAAt                                                                                                                                      <  2:2884033/140‑1 (MQ=255)

          gCTTACTGACGCTGCATGATAAATTGTTATTAGTCGGTTTCACAGGCGGGTTTAATCCATTTTTGACGCTGTCGTTTTTAGCTTTGCCTGTCTTTCCTGAC‑ATCAAAATGACGACAACAGGATTATTTGATGTAAAATCa                                                                                                                                    <  1:3351068/140‑1 (MQ=255)

          gCTTACTGACGCTGCATGATAAATTGTTATTAGTCGGTTTCACAGGCGGGTTTAATCCATTTTTGACGCTGTCGTTTTTAGCTTTGCCTGTCATTCCTGAC‑ATCAAAATGACGACAACAGGATTATTTGATGTAAAATCa                                                                                                                                    <  1:1509897/140‑1 (MQ=255)

          gCTTACTGACGCTGCATGATAAATTGTTATTAGTCGGTTTCACAGGCGGGTTTAATCCATTTTTGACGCTGTCGTTTTTAGCTTTGCCTGTCATTCCTGAC‑ATCAAAATGACGACAACAGGATTATTTGATGTAAAATCa                                                                                                                                    <  1:387934/140‑1 (MQ=255)

             tACTGACGCTGCATGATAAATTGTTATTAGTCGGTTTCACAGGCGGGTTTAATCCATTTTTGACGCTGTCGTTTTTAGCTTTGCCTGTCATTCCTGAC‑ATCAAAATGACGACAACAGGATTATTTGATGTAAAATCAttt                                                                                                                                 <  2:4065976/140‑1 (MQ=255)

             tACTGACGCTGCATGATAAATTGTTATTAGTCGGTTTCACAGGCGGGTTTAATCCATTTTTGACGCTGTCGTTTTTAGCTTTGCCTGTCATTCCTGAC‑ATCAAAATGACGACAACAGGATTATTTGATGTAAAATCAttt                                                                                                                                 <  2:2529448/140‑1 (MQ=255)

             tACTGACGCTGCATGATAAATTGTTATTAGTCGGTTTCACAGGCGGGTTTAATCCATTTTTGACGCTGTCGTTTTTAGCTTTGCCTGTCATTCCTGAC‑ATCAAAATGACGACAACAGGATTATTTGATGTAAAATCAttt                                                                                                                                 <  1:2764059/140‑1 (MQ=255)

             tACTGACGCTGCATGATAAATTGTTATTAGTCGGTTTCACAGGCGGGTTTAATCCATTTTTGACGCTGTCGTTTTTAGCTTTGCCGGTCATTCCTGAC‑ATCAAAATGACGACAACAGGATTATTTGATGTAAAATCAttt                                                                                                                                 <  2:2717727/140‑1 (MQ=255)

              aCTGACGCTGCATGATAAATTGTTATTAGTCGGTTTCACAGGCGGGTTTAATCCATTTTTGACGCTGTCGTTTTTATCGTTTCCTGTCATTCCTGAC‑ATAAAAATTACGACAACAGGATTATTTGATGTAAAATCAatta                                                                                                                                >  1:1665435/1‑139 (MQ=255)

              aCTGACGCTGCATGATAAATTGTTATTAGTCGGTTTCACAGGCGGGTTTAATCCATTTTTGACGCTGTCGTTTTTATATTTTACATTCATTACAGAC‑AAAAAAATTAAGACAAACGGATTAATTGAATTaaaaaaaatta                                                                                                                                >  1:3741830/1‑133 (MQ=255)

              aCTGACGCTGCATGATAAATTGTTATTAGTCGGTTTCACAGGCGGGTTTAATCCATTTTTGACGCTGTCGTTTTTAGCTTTGGCTGGGATTCCTGTC‑ATCAAAATTAAGACAACAGGTTTATTTTATGTAAAATCATTTc                                                                                                                                >  1:3942958/1‑140 (MQ=255)

              aCTGACGCTGCATGATAAATTGTTATTAGTCGGTTTCACAGGCGGGTTTAATCCATTTTTGACGCTGTCGTTTTTAGCTTTGCCTGTCATTCCTGAC‑ATCAAAATGACGACAACAGGATTATTTGATTTAAAATAAttta                                                                                                                                >  2:3451510/1‑139 (MQ=255)

               cTGACGCTGCATGATAAATTGTTCTTAGTCGGTTTCACAGGCGGGTTTAATCCATTATTGACGCTGTCGTTTTTAGCTTTGCCTGTCATTCCTGAC‑ATCAAAATGACGAAAACAGGATTATTTGATGTAAAATCATTTCa                                                                                                                               <  2:2450489/140‑1 (MQ=255)

               cTGACGCTGCATGATAAATTGTTATTAGTCGGTTTCACAGGCGGGTTTAATCCATTTTTGACGCTGTCGTTTTTAGCTTTGCCTGTCATTCCTGAC‑ATCAAAATGACGACAACAGGATTATTTGATGTAAAATCATTTCa                                                                                                                               <  1:579733/140‑1 (MQ=255)

               cTGACGCTGCATGATAAATTGTTATTAGTCGGTTTCACAGGCGGGTTTAATCCATTTTTGACGCTGTCGTTTTTAGCTTTGCCTGTCATTCCTGAC‑ATCAAAATGACGACAACAGGATTATTTGATGTAAAATCATTTCa                                                                                                                               <  2:3641755/140‑1 (MQ=255)

                  aCGCTGCATGATAAATTGTTATTAGTCGGTTTCACAGGCGGGTTTAATCCATTTTTGACGCTGTCGTTTTTAGCTTTGCCTGTCATTCCTGAC‑ATCAAAATGACGACAACAGGATTATTTGATGTAAAATCATTTCAGCa                                                                                                                            <  2:4025997/140‑1 (MQ=255)

                     cTGCATGATAAATTGTTATTAGTCGGTTTCACAGGCGGGTTTAATCCATTTTTGACGCTGTCGTTTTTAGCTTTGCCTGTCATTCCTGAC‑ATCAAAATGACGACAACAGGATTATTTGATGTAAAATCATTTCAGCat‑at                                                                                                                        >  2:3547672/1‑140 (MQ=255)

                     cTGCATGATAAATTGTTATTAGTCGGTTTCACAGGCGGGTTTAATCCATTTTTGACGCTGTCGTGTTTAGCTTTGCCTGTCATTCCTGAC‑ATCAAAATGACGACAACAGGATTATTTGATGTAAAATCATTTCAGCat‑at                                                                                                                        >  2:556653/1‑140 (MQ=255)

                        cATGATAAATTGTTATTAGTCGGTTTCACAGGCGGGTTTAATCCATTTTTGACGCTGTCGTTTTTAGCTTTTCCTGTCATTCCTGAC‑ATCAAAATGACGACAACAGGATTAGTTGATGTAAAATAATTTCAGCAT‑attgc                                                                                                                     >  2:4062515/1‑140 (MQ=255)

                             tAAATTGTTATTAGTCGGTTTCACAGGCGGGTTTAATCCATTTTTGACGCTGTCGTTTTTATCATTTCCAGTCAATCCTGAC‑ATAAAAATTAAGAAAACAGGAATATTTGATTTAAAATCATTTCAGCAT‑attgaatttc                                                                                                                >  1:4220283/1‑138 (MQ=255)

                              aaaTTGTTATTAGTCGGTTTCACAGGCGGGTTTAATACATTTTTGACGCTGTCGTTTTTAGCTTTGCCTGTCATTCCTGAC‑ATCAAAATGACGACAACAGGATTATTTGATGTAAAATCATTTCAGCAT‑Attgcattgca                                                                                                               <  2:3125034/140‑1 (MQ=255)

                                aTTGTTATTAGTCGGTTTCACAGGCGGGTTTAATCCATTTTTGACGCTGTCGTTTTTTGCTTTGCCTGTCATTCCTGAC‑ATCAAAATGACGACAACAGGATTATTTGATGTAAAATCATTTCAGCAT‑ATTGCATTGCAAt                                                                                                             >  2:3988040/1‑140 (MQ=255)

                                aTTGTTATTAGTCGGTTTCACAGGCGGGTTTAATCCATTTTTGACGCTGTCGTTTTTAGCTTTGCCTGTCATTCCTGAC‑ATCAAAATGACGACAACAGGATTATTTGATGTAAAATCATTTCAGCAT‑ATTGCATTGCAAt                                                                                                             >  2:1803923/1‑140 (MQ=255)

                                aTTGTTATTAGTCGGTTTCACAGGCGGGTTTAATCCATTTTTGACGCTGTCGTTTTTAGCTTTGCCTGTCATTCCTGAC‑ATCAAAATGACGACAACAGGATTATTTGATGTAAAAAAATTTCATCAT‑ATTTCATTTCAAt                                                                                                             >  2:3813683/1‑140 (MQ=255)

                                aTTGTTATTAGTCGGTTTCACAGGCGGGTTTAATCCATTTTTGACGCTGTCGTGTTTTTCTTTTCCTGTCATTCCTGAC‑ATCAAAAAGACGACAACAGGATTATTTGATGTAAAATCATTTAATCAT‑ATTTCATTGCAAt                                                                                                             >  1:2289671/1‑140 (MQ=255)

                                aTTGTTATTAGTCGGTTTCACAGGCGGGTTTAATCCATTTTTGACGCTGTCGTGTTTAGCTTTTCCTGTCATTCCTGAC‑ATCAAAAAGACGACAACAGGATTATTTGATTTAAAAACATTTAAGCAA‑ATTTGATTGCAAt                                                                                                             >  1:3551061/1‑140 (MQ=255)

                                aTTGTTATTAGTCGGTTTCACAGGCGGGTTTAATCCATTTTTGACGCTGTCGTGTTTAGCTTTTCCTGTCATTACTGAA‑ATCAAAATTACGACAACAGGATTATTTTATGTAAAATAATTTAAGCAT‑ATTTGATTTCAAt                                                                                                             >  1:1147419/1‑140 (MQ=255)

                                aTTGTTATTAGTCGGTTTCACAGGCGGGTTTAATCCATTTTTGACGCTGTCGTGTTTAGCTTTGCCTGTCATTCCTGAC‑ATCAAAATGACGACAACAGGATTATTTGATGTAAAATCATTTCAGCAT‑ATTGCATTGCAAt                                                                                                             >  2:4150997/1‑140 (MQ=255)

                                    ttatgaGTCTGTTTCACACGCGGGTTTAATCCATTTTTGACGCTGTCGTTTTTAAAATTTCCTTTCCTTCCTTACAAACAAAATTA‑GAAAAAAGGATTAATTGATTTAAAATCAATTGAACAT‑AATGCAATGCAATaaaa                                                                                                         >  2:81897/1‑140 (MQ=255)

                                     tattaGTCGGTTTCACAGGCGGGTTTAATCCATTTTTGACGCTGTCGTTTTTAGCTTTGCCTGTCATTCCTGAC‑ATCAAAATGACGAAAACAGGATTATTTGATGTAAAATCATTTCAGCAT‑ATTGCATTGCAATaaaaa                                                                                                        >  2:290169/1‑140 (MQ=255)

                                      attaGTCGGTTTCACAGGCGGGTTTAATCCATTTTTGACGCTGTCGTTTTTTTCTTTTCCGTTCATTACTGAC‑ATAAAAATGTAGACAACAGGTTTATTTTATGTTAAATCATTTCAGCAA‑ATTTCATTGCAATaaaaaa                                                                                                       >  1:2169153/1‑140 (MQ=255)

                                      atgaGTCGGTGTCACAGGCGGGCTGAATCCATTTTTGACGCTGTCGTTTTTAGCTGTGCCGGTCATTCCTGAC‑ATCAAAATGACGACAACAGGATTATTTGATGTAAAATCATTTCAGCAT‑CTTGCATTGCAATaaaaaa                                                                                                       <  1:2668753/137‑1 (MQ=255)

                                         aGTCGGTTTCACAGGCGGGTTTAATCCATTTTTGACGCTGTCGTTTTTAGCTTTTCCTGTCATTACTGAA‑ATCAAAATTACGACAACAGGATTATTTTATGTAAAATAATTTAACAAT‑ATTGCATTGAAATAAAAAAAGa                                                                                                    >  1:2397968/1‑140 (MQ=255)

                                          gTCGGTTTCACAGGCGGGTTTAATCCATTTTTGACGCTGTCGTGTTTAGCTTTTCCTGTAATTACTGAA‑AAAAAAAAGACGACAACAGGATTATTTTATGTAAAATAATTTAAGCATAATTACA‑TGCAATAAAAAAAAAt                                                                                                   >  1:9790/1‑140 (MQ=255)

                                           tctgTTTCACAGGCGGGTTTAATCCATTTTTGACGCTGTCGTTTTTAGCTTTGCCTGTCATTCCTGAC‑ATCAAAATGACGACAACAGGATTATTTGATGTAAAATCATTTCAGCAT‑ATTGCATTGCAATAAAAAAAGATg                                                                                                  <  2:1828844/137‑1 (MQ=255)

                                           tCGGTTTCACAGGCGGGTTTAATCCATTTTTGACGCTGTCGTTTTTATCTTTGCCTGTCATTACTGAC‑ATCAAAATGAAGACAACAGGATTATTTGATTTAAAATAATTTAAGCAA‑ATTTCATTTCAATAAAAAAAAATg                                                                                                  >  2:1235332/1‑140 (MQ=255)

                                            cGGTTTCACAGGCGGGTTTAATCCATTTTTGACGCTGTCGTTTTTAGCTTTGCCTGTCATTCCTGAC‑ATCAAAATGACGACAACAGGATTATTTGATGTAAAATCATTTCAGCAT‑ATTGCATTGCAATAAAAAAAGATGc                                                                                                 <  2:2972584/140‑1 (MQ=255)

                                            cGGTTTCACAGGCGGCTTTAAAACATTTTGTACGCTGTCGTTTTTAGCTGTGCCTCTCAGTCCTGAG‑ATCAAAATGACGCCAACAGGATTATTTTATGTAAAATCATTTCAGCCT‑AGTGCATTGCATTAAAAAAACATga                                                                                                 <  2:514969/140‑2 (MQ=255)

                                              gTTTCACAGGCGGGTTTAATCCATTTTTGACGCTGGCGTGTTTTGCGTTGCCTGTGATTCCATTC‑ATCAAAATGACGACAACACGAATGTTTGATGTACAAACAGTGCAGCAA‑ATAGGAGTGCAATAAAAAAAGAttacg                                                                                               >  2:2853750/1‑136 (MQ=255)

                                                      ggCGGGTTTAATCCATTTTTGACGCTGTCGTTTTTAGCTTTGCCTGTCATTCCTGAC‑ATCAAAATGACGACAACAGGATTATTTGATGTAAAATCATTTCAGCAT‑ATTGCATTGCAATAAAAAAAGATGCACTCCAAagt                                                                                       <  2:1924044/140‑1 (MQ=255)

                                                      cgCGGGTTTAATCCATTTTTGACGCTGACGTTTATAGCTTTGCCTGTCATTCCTGAC‑ATCAAAATGACGACAACAGGATTATTTGATGTAAAAACATTTCAGCAT‑ATTGCATTGCAATAAAAAAAGATGCACTCCAAagt                                                                                       <  2:3393734/139‑1 (MQ=255)

                                                       gCGGGTTTAATCCATTTTTGACGCTGTCGTTTTTAGCTTTGCCTGTCATTCCTGAC‑ATCAAAATGACGACAACAGGATTATTTGATGTAAAATCATTTCAGCAT‑ATTGCATTGCAATAAAAAAAGATGCACTCCAAagtg                                                                                      <  1:1010260/140‑1 (MQ=255)

                                                           gTTTAATCCATTTTTGACGCTGTCGTTTTTAGCTTTGCCTGTCATTCCTGAC‑ATCAAAATGACGACAACAGGCTTATTTGATGTAAAATCATTTAAGCAT‑ATTGCCGTGCAATAAAAATAGATGCAATCCAAagtgagtg                                                                                  >  2:1061351/1‑140 (MQ=255)

                                                            tttAATCCATTTTTGACGCTGTCGTTTTTAGCTTTGCCTGTCATTCCTGAC‑ATCAAAATGACGACAACAGGATTATTTGATGTAAAATCATTTAAGCAT‑ATTTCATTGCAATAAAAAAATATGCACTACAAAGTGAGTGc                                                                                 >  2:872532/1‑140 (MQ=255)

                                                            tttAATCCATTTTTGACGCTGTCGTGTTTAGCTTTGCCTGTCATTCCTGAC‑ATCAAAAAGACGACAACAGGATTATTTTATGTAAAATAATTTCAGCAT‑ATTGCATTTCAAAAAGAAAAGATGCACTCCAAAGTGAGTGc                                                                                 >  1:3889677/1‑140 (MQ=255)

                                                                aTCCATTGTTGACGCGGGCGTTTTTAGCTTTCCCTGTCATTCCTGAC‑ATCACAATGACGACAACCGGATTATTTGATGTAAAAGCATTTCAGCAT‑ATAGCATTGCAATAAAAAAAGATGCACTCCACAGTGAGTGCATC‑t                                                                            <  2:3401964/140‑1 (MQ=255)

                                                                     tttttGACGCTGTCGTTTTTAGCTTTGCCTGTAATTCCTGAC‑ATCAAAATGAAGACAACACGATTATTTTATGTAAAATCAATTAAGCAT‑ATTGCAATGCAATAAAAAAAGATGCAATACAAAGTGAGTGAATC‑tttttt                                                                       >  2:229933/1‑140 (MQ=255)

                                                                        ttGACGCTGTCGTTTTTATCTTTGCCTGTCATTCCTCAC‑ATCAAAATGACGACAACAGGATTATTTGATGTAAAATCATTTCAGCAT‑ATTGCATTGCAATAAAACAAGATGCACTCCAAAGTGAGTGCATC‑TTTTTTTTa                                                                    <  1:859430/140‑1 (MQ=255)

                                                                        ttGACGCTGTCGTTTTTAGCTTTGCCTGTCATTCCTGAC‑ATCAAAATGACGACAACAGGATTATTTGATGTAAAATCATTTCAGCAT‑ATTGCATTGCAATAAAAAAAGATGCACTCCAAAGTGAGTGCATC‑TTTTTTTTa                                                                    <  1:1442741/140‑1 (MQ=255)

                                                                        ttGACGCTGTCGTTTTTAGCTTTGCCTGTCATTCCTGAA‑ATCAAAATGACGACAACAGGATTATTTGATGAAAAATCATTTCACCAT‑ATTGCATTGCAATAAAAAAAGATGCACTCCAAACTGAGTGCATC‑TTTTTTTTa                                                                    <  1:2306646/140‑1 (MQ=255)

                                                                                    tttttAGCTTTGCCTGTCATTCCTGAC‑ATCAAAATGACGACAACAGGATTATTTGATGTAAAATCATTTCAGCAT‑ATTGCATTGCAATAAAAAAAGATGCACTCCAAAGTGAGTGCATC‑TTTTTTTTAAACTTCCatgat                                                        <  2:1427972/140‑1 (MQ=255)

                                                                                    tttttAGCTTTGCCTGTCATTCCTGAC‑ATCAAAATGACGACAACAGGATTATTTGATGTAAAATCATTTCAGCAT‑ATTGCATTGCAATAAAAAAAGATGCACTCCAAAGTGAGTGCATC‑TTTTTTTTAAACTTCCatgat                                                        <  2:2992714/140‑1 (MQ=255)

                                                                                    tttttAGCTTTGCCTGTCATTCCTGAC‑ATCAAAATGACGACAACAGGATTATTTGATGTAAAATCATTTCAGCAT‑ATTGCATTGCAATAAAAAAAGATGCACTCCAAAGTGAGTGCATC‑TTTTTTTTAAACTTCCatgat                                                        <  2:2095697/140‑1 (MQ=255)

                                                                                           ctgtGCCTGTCATTCCTGAC‑ATCAAAATGACGACAACAGGATTATTTGATGTAAAATCATTTCAGCAT‑ATTGCATTGCAATAAAAAAAGATGCACTCCAAAGTGAGTGCATC‑TTTTTTTTAAACTTCCATGATGATCGGa                                                 <  2:2397968/137‑1 (MQ=255)

                                                                                           cTTTGCCTGTCATTCCTGAC‑ATCAAAATGACGACAACAGGATTATTTGATGTAAAATCATTTCAGCAT‑ATTGCATTGCAATAAAAAAAGATGCACTCCAAAGTGAGTGCATC‑TTTTTTTTAAACTTCCATGATGATCGGa                                                 <  2:2274367/140‑1 (MQ=255)

                                                                                           cTTTGCCTGTCATTCCTGAC‑ATCAAAATGACGACAACAGGATTATTTGATGTAAAATCATTTCAGCAT‑ATTGCATTGCAATAAAAAAAGATGCACTCCAAAGTGAGTGCATC‑TTTTTTTTAAACTTCCATGATGATCGGa                                                 <  1:3813683/140‑1 (MQ=255)

                                                                                           cTTTGCCTGTCATTCCTGAC‑ATCAAAATGACGACAACAGGATTATTTGATGTAAAATCATTTCAGCAT‑ATTGCATTGCAATAAAAAAAGATGCACTCCAAAGTGAGTGCATC‑TTTTTTTTAAACTTCCATGATGATCGGa                                                 >  2:781491/1‑140 (MQ=255)

                                                                                           cTTTGCCTGTCATTCCTGAC‑ATCAAAATGACGACAACAGGATTATTTGATGTAAAATCATTTCAGCAT‑ATTGCATTGCAATAAAAAAAGATGCACTCCAAAGTGAGTGCATC‑TTTTTTTTAAACTTCCATGATGATCGGa                                                 <  2:786273/140‑1 (MQ=255)

                                                                                           cTTTGCCTGTCATTCCTGAC‑ATCAAAATGACGACAACAGGATTATTTGATGTAAAATCATTTCAGCAT‑ATTGCATTGCAATAAAAAAAGATGCACTCCAAAGTGAGTGCATC‑TTTTTTTTAAACTTCCATGATGATCGGa                                                 <  1:789696/140‑1 (MQ=255)

                                                                                           cTTTGCCTGTCATTCCTGAC‑ATCAAAATGACGACAACAGGATTATTTGATGTAAAATCATTTCAGCAT‑ATTGCATTGCAATAAAAAAAGAGGCACTCCAAAGTGAGTGCATC‑TTTTTTTTAAACTTCCATGATGATCGGa                                                 <  1:4150997/140‑1 (MQ=255)

                                                                                            tttGCCTGTCATTCCTGAC‑ATCAAAATGACGACAACAGGATTATTTGATGTAAAATCATTTCAGCAT‑ATTGCATTGCAATAAAAAAAGATGCACTCCAAAGTGAGTGCATCTTTTTTTTTAAACTTCCATGATGATCGGa                                                 <  1:1803923/140‑1 (MQ=255)

                                                                                               gCCTGTCATTCCTGAC‑ATCAAAATGACGACAACAGGATTATTTGATGTAAAATCATTTCAGCAT‑ATTGCATTGCAATAAAAAAAGATGCACTCCAAAGTGAGTGCATC‑TTTTTTTTAAACTTCCATGATGATCGGAAGGa                                             <  1:2098693/140‑1 (MQ=255)

                                                                                               gCCTGTCATTCCTGAC‑ATCAAAATGACGACAACAGGATTATTTGATGTAAAATCATTTCAGCAT‑ATTGCATTGCAATAAAAAAAGATGCACTCCAAAGTGAGTGCATC‑TTTTTTTTAAACTTCCATGATGATCGGAAGGa                                             <  1:556653/140‑1 (MQ=255)

                                                                                               gCCTGTCATTCCTGAC‑ATCAAAATGACGACAACAGGATTATTTGATGTAAAATCATTTCAGCAT‑ATTGCATTGCAATAAAAAAAGATGCACTCCAAAGTGAGTGCATC‑TTTTTTTTAAACTTCCATGATGATCGGAAGGa                                             <  1:4314085/140‑1 (MQ=255)

                                                                                               gCCTGTCATTCCTGAC‑ATCAAAATGACGACAACAGGATTATTTGATGTAAAATCATTTCAGCAT‑ATTGCATTGCAATAAAAAAAGATGCACTCCAAAGTGAGTGCATC‑TTTTTTTTAAACTTCCATGATGATCGGAAGGa                                             <  2:1382756/140‑1 (MQ=255)

                                                                                               gCCTGTCATTCCTGAC‑ATCAAAATGACGACAACAGGATTATTTGATGTAAAATCATTTCAGCAT‑ATTGCATTGCAATAAAAAAAGATGCACTCCAAAGTGAGTGCATC‑TTTTTTTTAAACTTCCATGATGATCGGAAGGa                                             <  2:1382617/140‑1 (MQ=255)

                                                                                               gCCTGTCATTCCTGAC‑ATAAAAATGACGACAACAGGATTATTTGATGTAAAATCATTTCAGCAT‑ATTGCATTGCAATAAAAAAAGATGCACTCCAAAGTGAGTGCATC‑TTTTTTTTAAACTTCCATGATGATCGGAAGGa                                             <  2:2222231/140‑1 (MQ=255)

                                                                                                       ttCCTGAC‑ATCAAAATGACGACAACAGGATTATTTGATGTAAAATCATTTCAGCAT‑ATTGCATTGCAATAAAAAAAGATGCACTCCAAAGTGAGTGCATC‑TTTTTTTTAAACTTCCATGATGATCGGAAGGATCATCGGa                                     <  1:1021191/140‑1 (MQ=255)

                                                                                                         ccTGAC‑ATCAAAATGACGACAACAGGATTATTTGATGTAAAATCATTTCAGCAT‑ATTGCATTGCAATAAAAAAAGATGCACTCCAAAGTGAGTGCATC‑TTTTTTTTAAACTTCCATGATGATCGGAAGGATCATCGGACg                                   <  2:4220283/140‑1 (MQ=255)

                                                                                                         ccTGAC‑ATCAAAATGACGACAACAGGATTATTTGATGTAAAATCATTTCAGCAT‑ATTGCATTGCAATAAAAAAAGATGCACTCCAAAGTGAGTGCATC‑TTTTTTTTAAACTTCCATGATGATCGGAAGGATCATCGGACg                                   <  2:1434847/140‑1 (MQ=255)

                                                                                                         ccTGAC‑ATCAAAATGACGACAACAGGATTATTTGATGTAAAATCATTTCAGCAT‑ATTGCATTGCAATAAAAAAAGATGCACTCCAAAGTGAGTGCATC‑TTTTTTTTAAACTTCCATGATGATCGGAAGGATCATCGGACg                                   <  2:118311/140‑1 (MQ=255)

                                                                                                                aTCAAAATGACGACAACAGGATTATTTGATGTAAAATCATTTCAGCAT‑ATTGCATTGCAATAAAAAAAGATGCACTCCAAAGTGAGTGCATC‑TTTTTTTTAAACTTCCATGATGATCGGAAGGATCATCGGACGGcgttt                             >  1:1777649/1‑140 (MQ=255)

                                                                                                                     aaTGACGACAAGAGGATTATTTGATGTAAAATCATTTCAGCAT‑ATTGCATTGCAATAAAAAAAGATGCACTCCAAAGTGAGTGCATC‑TTTTTTTTAAACTTCCATGATGATCGGAAGGATCATCGGACGGcgtttcgttt                        <  1:1235332/140‑1 (MQ=255)

                                                                                                                       tGACGACAACAGGATTATTTGATGTAAAATCATTTCAGCAT‑ATTGCATTGCAATAAAAAAAGATGCACTCCAAAGTGAGTGCATC‑TTTTTTTTAAACTTCCATGATGATCGGAAGGATCATCGGACGGCGTTTCGttttt                      <  2:2822905/140‑1 (MQ=255)

                                                                                                                       tGACGACAACAGGATTATTTGATGTAAAATCATTTCAGCAT‑ATTGCATTGCAATAAAAAAAGATGCACTCCAAAGTGAGTGCATC‑TTTTTTTTAAACTTCCATGATGATCGGAAGGATCATCGGACGGCGTTTCGttttt                      <  2:517502/140‑1 (MQ=255)

                                                                                                                        gacgacAACAGGATTATTTGATGTAAAATCATTTCAGCAT‑ATTGCATTGCAATAAAAAAAGATGCACTCCAAAGTGAGTGCATC‑TTTTTTTTAAACTTCCATGATGATCGGAAGGATCATCGGACGGCGTTTCGtttttt                     <  1:1256408/140‑1 (MQ=255)

                                                                                                                          cgacAACAGGATTATTTGATGTAAAATCATTTCAGCAT‑ATTGCATTGCAATAAAAAAAGATGCACTCCAAAGTGAGTGCATC‑TTTTTTTTAAACTTCCATGATGATCGGAAGGATCATCGGACGGCGTTTCGTTTTTTCa                   <  1:864143/140‑1 (MQ=255)

                                                                                                                          cgacAACAGGATTATTTGATGTAAAATCATTTCAGCAT‑ATTGCATTGCAATAAAAAAAGATGCACTCCAAAGTGAGTGCATC‑TTTTTTTTAAACTTCCATGATGATCGGAAGGATCATCGGACGGCGTTTCGTTTTTTCa                   >  1:3786896/1‑140 (MQ=255)

                                                                                                                          cgacAACAGGATTATTTGATGTAAAATCATGTCAGCAT‑ATTGCATTGCAATAAAAAAAGATGCACTCCAAAGTGAGTGCATC‑TTTTTTTTAAACTTCCATGATGATCGGAAGGATCATCGGACGGCGTTTCGTTTTTTCa                   <  1:4065678/140‑1 (MQ=255)

                                                                                                                          cgaaAACAGTATTATTTGATGTAAAATCATTTCAGAAT‑ATTGCATTGAAATAAAAAAAGTTGCACTCCAAAGTGAGTGCATC‑TTTTTTTTAAACTTCCATGATGATCGGAAGGATCATCGGACGGCGTTTCGTTTTTTCa                   <  1:1953708/140‑1 (MQ=255)

                                                                                                                             caacaTGATTATTTGATGTAAAATCATTTCAGCAT‑ATTGCATTGCAATAAAAAAAGATGCACTCCAAAGTGAGTGCATC‑TTTTTTTTAAACTTCCATGATGATCGGAAGGATCATCGGACGGCGTTTCGTTTTTTCatat                <  2:1071386/140‑1 (MQ=255)

                                                                                                                                  tgATTATTTGATGTAAAATCATTTCAGCAT‑ATTGCATTGCAATAAAAAAAGATGCACTCCAAAGTGAGTGCATC‑TTTTTTTTAAACTTCCATGATGATCGGAAGGATCATCGGACGGCGTTTCGTTTTTTCATATAAGaa           <  1:3451510/139‑1 (MQ=255)

                                                                                                                                  gtattattTGATGTAAAATCATTTCAGCAT‑ATTGCATTGCAATAAAAAAAGATGCACTCCAAAGTGAGTGCATC‑TTTTTTTTAAACTTCCATGATGATCGGAAGGATCATCGGACGGCGTTTCGTTTTTTCATATAAGaa           <  2:1177109/138‑1 (MQ=255)

                                                                                                                                  gtattattTGATGTAAAATCATTTCAGCAT‑ATTGCATTGCAATAAAAAAAGATGCACTCCAAAGTGAGTGCATC‑TTTTTTTTAAACTTCCATGATGATCGGAAGGATCATCGGACGGCGTTTCGTTTTTTCATATAAGaa           <  2:1147419/138‑1 (MQ=255)

                                                                                                                                  ggATTATTTGATTTAAAATCATTTCAGCAT‑ATTGCATTGCAATAAAAAAAGATGCACTCCAAAGTGAGTGCATC‑TTTTTTTTAAACTTCCATGATGATCGGAAGGATCATCGGACGGCGTTTCGTTTTTTCATATAAGaa           <  2:3175280/140‑1 (MQ=255)

                                                                                                                                  ggATTATTTGATGTAAAATCATTTCAGCAT‑ATTGCATTGCAATAAAAAAAGATGCACTCCAAAGTGAGTGCATC‑TTTTTTTTAAACTTCCATGATGATCGGAAGGATCATCGGACGGCGTTTCGTTTTTTCATATAAGaa           <  2:2458365/140‑1 (MQ=255)

                                                                                                                                  ggATTATTTGATGTAAAATCATTTCAGCAT‑ATTGCATTGCAATAAAAAAAGATGCACTCCAAAGTGAGTGCATC‑TTTTTTTTAAACTTCCATGATGATCGGAAGGATCATCGGACGGCGTTTCGTTTTTTCATATAAGaa           <  2:268780/140‑1 (MQ=255)

                                                                                                                                  ggATTATTTGATGTAAAATCATTTCAGCAT‑ATTGCATTGCAATAAAAAAAGATGCACTCCAAAGTGAGTGCATC‑TTTTTTTTAAACTTCCATGATGATCGGAAGGATCATCGGACGGCGTTTCGTTTTTTCATATAAGaa           <  2:3551061/140‑1 (MQ=255)

                                                                                                                                  ggATTATTTGATGTAAAATCATTTCAGCAT‑ATTGCATTGCAATAAAAAAAGATGCACTCCAAAGTGAGTGCATC‑TTTTTTTTAAACTTCCATGATGATCGGAAGGATCATCGGACGGCGTTTCGTTTTTTCATATAAGaa           <  2:268875/140‑1 (MQ=255)

                                                                                                                                   gATTATTTGATGTAAAATCATTTCAGCAT‑ATTGCATTGCAATAAAAAAAGATGCACTCCAAAGTGAGTGCATC‑TTTTTTTTAAACTTCCATGATGATCGGAAGGATCATCGGACGGCGTTTCGTTTTTTCATATAAGAAc          >  2:282404/1‑140 (MQ=255)

                                                                                                                                   gATTATTTGATGTAAAATCATTTCAGCAT‑ATTGCATTGCAATAAAAAAAGATGCACTCCAAAGTGAGTGCATC‑TTTTTTTTAAACTTCCATGATGATAGGAAGGATCATCGGACGGCGTTTCGTTTTTTCATCTAAGAAc          >  2:3462104/1‑140 (MQ=255)

                                                                                                                                        tttGATGTAAAATCATTTCAGCAT‑ATTGCATTGCAATAAAAAAAGATGCACTCCAAAGTGAGTGCATC‑TTTTTTTTAAACTTCCATGATGATCGGAAGGATCATCGGACGGCGTTTCGTTTTTTCATATAAGAACGgcgc     >  1:2679634/1‑140 (MQ=255)

                                                                                                                                           gATGTAAAATCATTTCAGCAT‑ATTGCATTGCAATAAAAAAAGATGCACTCCAAAGTGAGTGCATC‑TTTTTTTTAAACTTCCATGATGATCGGAAGGATCATCGGACGGCGTTTCGTTTTTTCATATAAGAACGGCGCAAg  <  1:290169/140‑1 (MQ=255)

GTGAATCAAAGCTTACTGACGCTGCATGATAAATTGTTATTAGTCGGTTTCACAGGCGGGTTTAATCCATTTTTGACGCTGTCGTTTTTAGCTTTGCCTGTCATTCCTGAC‑ATCAAAATGACGACAACAGGATTATTTGATGTAAAATCATTTCAGCAT‑ATTGCATTGCAATAAAAAAAGATGCACTCCAAAGTGAGTGCATC‑TTTTTTTTAAACTTCCATGATGATCGGAAGGATCATCGGACGGCGTTTCGTTTTTTCATATAAGAACGGCGCAAG  >  NZ_CP034943/1488020‑1488297

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`