BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	1,514,881	G→T	7.4%	Q62K (CAA→AAA)	EO946_RS07920 ←	YlaI family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	1,514,881	0	G	T	7.4%	677.9 / 8.7	252	Q62K (CAA→AAA)	EO946_RS07920	YlaI family protein
Reads supporting (aligned to +/- strand): ref base G (46/179); new base T (25/2); total (71/181)
Fisher's exact test for biased strand distribution p-value = 1.11e-13
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 255 total aligned reads displayed.

CATTTACAAAATTCGGCTGTATCAGTCGAAAAAGGGACAGTCTGCAAAATCATAAAAAGACTCAGCTGAGAAGCTGAGTCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTT‑TTT‑C‑TTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGCACATATATGTATGGATTGGGCGGTTTCGAAGCCGTTTCGCGATCAGGGTATC  >  NZ_CP034943/1514742‑1515013

cATTTACAAAATTCGGCTGTATCAGTCGAAAAAGGGACAGTCTGCAAAATCATAAAAAGACTCAGCTGAGAAGCTGAGTCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTT‑TTT‑C‑TTTTTTTTg                                                                                                                                      <  1:4086273/140‑1 (MQ=255)

  tttACAAAATTCGGCTGTATCAGTCGAAAAAGGGACAGTCTGCAAAATCATAAAAAGACTCAGCTGAGAAGCTGAGTCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCGTTATTTTTTGACTT‑TTT‑G‑TTTTTTTTTCt                                                                                                                                    >  2:3817005/1‑140 (MQ=255)

  tttACAAAATTCGGCTGTATCAGTCGAAAAAGGGACAGTCTGCAAAATCATAAAAAGACTCAGCTGAGAAGCTGAGTCTGTTTTTGTGTTTTGTATGGTAAAGCGGCCGCTTTAGTTTTTGACTT‑TTT‑C‑TTTTTTTTTCt                                                                                                                                    >  2:1107011/1‑140 (MQ=255)

  tttACAAAAATCGGCTGTATCAGTCGAAAAAGGGACAGTCTGCAAAATCATAAAAAGACTCAGCTGAGAAGCTGAGTCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCTTTAATTTTTGACTT‑TTT‑CATTTTTTTTGc                                                                                                                                     >  2:4226869/1‑140 (MQ=255)

      cAAAATTCGGCTGTATGTGTAGAAAAAGGTACAGTCTGCAAAATCATAAATATACTCTGCTGAGAAGCTTATTCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCTCTTTATTTTTTGACTT‑TTT‑C‑TTTTTTTTGCTGTcc                                                                                                                                <  2:588818/140‑1 (MQ=255)

      cAAAATTCGGCTGTATCAGTCGAAAAAGGGACAGTCTGCAAAATCATAAAAAGACTCAGCTGAGAAGCTGAGTCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTT‑TTT‑C‑TTTTTTTTGCTGTcc                                                                                                                                <  2:2072331/140‑1 (MQ=255)

                        gTCGAAAAAGGGACAGTCTGCAAAATCATAAAAAGACTCAGCTGAGAAGCTGAGTCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCTTGTTTTTTTGTCTT‑TTTGT‑TTTTTTTTGGTGTACCGGGTGAAAAGTGAAtt                                                                                                               >  1:520250/1‑140 (MQ=255)

                        gTCGAAAAAGGGACAGTCTGCAAAATCATAAAAAGACTCAGCTGAGAAGCTGAGTCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCGTTATTTTTTGACTT‑TTT‑G‑TTTTTTTTTCTTTCCCGGGTGAAAAGTGAAttt                                                                                                              >  1:1341624/1‑140 (MQ=255)

                          cGAAAAAGGGACAGTCTGCAAAATCATAAAAAGACTCAGCTGAGAAGCTGAGTCTTTTTTTGTGTTTTGTATGGTAAATCGGCCGCTTTATTTTTTTCCTT‑TTT‑C‑TTTTTTTTGCTGTCCCGGGTGAAAAGTGACTTTTc                                                                                                            >  2:2952461/1‑140 (MQ=255)

                             aaaaGGGACAGTCTGCAAAATCATAAAAAGACTCAGCTGAGAAGCTGAGTCTTTTTTTGGGGTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTTGTTT‑T‑TTTGTTTTGTTG‑GCCGGGGGTAAAGTGTATTTTGCCg                                                                                                         >  1:3528286/1‑140 (MQ=255)

                                  ggACAGTCTGCAAAATCATAAAAAGACTCAGCTGAGAAGCTGAGTCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTT‑TTT‑C‑TTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTc                                                                                                    <  1:3214976/140‑1 (MQ=255)

                                     cAGTCTGCAAAATCATAAAAAGACTCAGCTGAGAAGCTGAGTCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCGTTTTTTTTTGTCTT‑TTT‑G‑TTTTTTTTGGTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTGAGc                                                                                                 >  1:3843337/1‑140 (MQ=255)

                                     cAGTCTGCAAAATCATAAAAAGACTCAGCTGAGAAGCTGAGTCTTTTTTGGTGTTTTGTATGGTAAAGCGGCCGCGGTTTTTTTTGACTT‑TTT‑G‑TTTTTTTTGGTGTCCCGGGTGTAAAGTGAATTTTCCCGTTTTGAGc                                                                                                 >  1:270954/1‑140 (MQ=255)

                                       gTCTGCAAAATCATAAAAAGACTCAGCTGAGAAGCTGAGTCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTTACTT‑TTT‑C‑TTTTTTTTGCTGTCCCGGGTGAAAATTGAATTTTCCCGTTTTAAGCCg                                                                                               >  2:3170616/1‑140 (MQ=255)

                                       gTCTGCAAAATCATAAAAAGACTCAGCTGAGAAGCTGAGTCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTT‑TTT‑C‑TTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCg                                                                                               >  2:2223437/1‑140 (MQ=255)

                                          tGCAAAATCATAAAAAGACTCAGCTGAGAAGGTGAGTCTTTTTTTGTGTTTTGGATGGTAAAGCGGCCGCGGTGTTGTTTTACGT‑TTT‑G‑GTTTTTTTTCTGTAACGGGTGTAACTTGAATTTTCCCGGGTTccgccggtg                                                                                            >  1:815983/1‑137 (MQ=255)

                                          tGCAAAATCATAAAAAGACTCAGCTGAGAAGCTGAGTCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTT‑TTT‑C‑TTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGtta                                                                                            >  2:1588021/1‑139 (MQ=255)

                                              aaaTCATAAAAAGACTCAGCTGAGAAGCTGAGTCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTT‑TTT‑C‑TTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTtcttct                                                                                        <  2:535837/140‑1 (MQ=255)

                                                  cATAAAAAGACTCAGCTGAGAAGCTGAGTCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTT‑TTT‑C‑TTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGttt                                                                                    >  2:4188607/1‑140 (MQ=255)

                                                   aTAAAAAGACTCAGCTGAGAAGCTGAGTCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTT‑TTT‑C‑TTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGtttt                                                                                   <  1:94455/140‑1 (MQ=255)

                                                   aTAAAAAGACTCAGCTGAGAAGCTGAGTCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTT‑TTT‑C‑TTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTGTCCCGTTTTCAGCCGTTCTTCTGtttt                                                                                   <  1:1772795/140‑1 (MQ=255)

                                                    tAAAAAGACTCAGCTGAGAAGCTGAGTCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTT‑TTT‑G‑TTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTGCCGGTTTGAGCCGGTCTTCTGttttt                                                                                  >  2:3293746/1‑140 (MQ=255)

                                                    tAAAAAGACTCAGCTGAGAAGCTGAGTCTTTTTTTGTGTTTTGGATGGTAAAGCGGCCGCGTTATTTTTTGTCTT‑TTT‑G‑TTTTTTTTTGTGTGCCGGGTGTAAAGTGAATTTTGCCGTTTTGATGCGGTGTTGTGttttt                                                                                  >  1:3411897/1‑140 (MQ=255)

                                                    tAAAAAGACTAAGCTGAGAAGCTGAGTCTTTTTGTGTGTTGTGTATGGTAAATCGGCCGCTTTATTTTTTGACTT‑TTT‑A‑TTTTTTTTGCTGTAACGGGTGAAAAGTGAATTTTCCCGTTTTGGTCCGTTGTTGTGttttt                                                                                  >  2:186005/1‑140 (MQ=255)

                                                        aaGACTCAGCTGAGAAGCTGTGTCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTT‑TTT‑C‑TTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTggg                                                                              <  1:836120/140‑1 (MQ=255)

                                                        aaGACTCAGCTGAGAAGCTGAGTCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTT‑TTT‑C‑TTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTggg                                                                              <  1:3696317/140‑1 (MQ=255)

                                                             tCAGCTGAGAAGCTGAGTCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTT‑TTT‑C‑TTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATc                                                                         <  2:194472/140‑1 (MQ=255)

                                                              cAGCTGAGAAGCTGAGTCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTT‑TTT‑CTTTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATc                                                                         <  2:3520813/140‑1 (MQ=255)

                                                                 cTGAGAAGCTGAGTCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTT‑TTT‑C‑TTTTTTTTGCTGTCCCGGGTGAAAAGTTAATTTTCCCCTTTTCAGGAGTTCTTCTTTTTTTTGGTCACTCCGGt                                                                     >  2:2442868/1‑140 (MQ=255)

                                                                     gaAGCTGATTATTTTTTTGTGTTTTGTATGGTAAAGCGGACGCTTTATTTTTTGACTT‑TTT‑C‑TTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTg                                                                 <  1:1073690/140‑1 (MQ=255)

                                                                      aaGCTGAGTCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTT‑TTT‑C‑TTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTgg                                                                <  2:489906/140‑1 (MQ=255)

                                                                      aaGCTGAGTCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTT‑TTT‑C‑TTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTgg                                                                <  2:1742671/140‑1 (MQ=255)

                                                                       aGCTGAGTCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTT‑TTT‑C‑TTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGc                                                               <  2:379769/140‑1 (MQ=255)

                                                                       aGCTGAGTCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTT‑TTT‑C‑TTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGc                                                               <  2:2356854/140‑1 (MQ=255)

                                                                       aGCTGAGTCTTTTTCTGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTT‑TTT‑C‑TTTTTTGTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGc                                                               <  2:2926172/140‑1 (MQ=255)

                                                                         cTGAGTCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTTACTT‑TTT‑G‑TTTTTTTTTGTGTGCCCGGTGAAAAGTGAAATTTACCGGTTTCAGCCGTTCTTCTGTTTTTTTGGCAAAGCGGTCGTGGGAt                                                             >  1:3344871/1‑140 (MQ=255)

                                                                           gAGTCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTT‑TTT‑C‑TTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGTGCAATACGGTCGTGGCATTc                                                           >  2:4303261/1‑140 (MQ=255)

                                                                           gAGTCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTT‑TTT‑C‑TTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTc                                                           <  1:1204797/140‑1 (MQ=255)

                                                                           gAGTCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCGTTTTTTTTTTTCTT‑TTT‑T‑TTTTTTTTTCTTTACCCGGTGAAAAGTTAATTTTACCCTTTTAAGCCGTTCTTCTGTTTTTTTTGCAATCACGTCGTGGCAATc                                                           >  1:1967984/1‑140 (MQ=255)

                                                                           gAGTCTTTTTTTGTGTTGTGTATGGTAAAGCGGCCGCTGTATTTTTTGCCTT‑TTT‑C‑TTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTGCCCGTTTTCAGCCGTTCTTCTGTTTATTGGGCAATCCGGTCGTGGCATTc                                                           <  1:3767294/140‑1 (MQ=255)

                                                                            aGTCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTT‑TTT‑C‑TTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTcc                                                          <  2:145214/140‑1 (MQ=255)

                                                                            aGTCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTT‑TTT‑C‑TTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTcc                                                          <  2:1425895/140‑1 (MQ=255)

                                                                              tCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTT‑TGT‑C‑TTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTg                                                        <  2:1560609/140‑1 (MQ=255)

                                                                               cTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTT‑TTT‑C‑TTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTgg                                                       <  1:34658/140‑1 (MQ=255)

                                                                               cTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTT‑TTT‑C‑TTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTgg                                                       <  2:793108/140‑1 (MQ=255)

                                                                               cTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTT‑TTT‑C‑TTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTgg                                                       <  1:4224607/140‑1 (MQ=255)

                                                                               cTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTT‑TTT‑C‑TTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTgg                                                       <  2:825936/140‑1 (MQ=255)

                                                                               cTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTT‑GTT‑C‑TTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCATCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTgg                                                       <  1:4237658/140‑1 (MQ=255)

                                                                               atttttttGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTT‑TTT‑C‑TTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTgg                                                       <  2:3114369/139‑1 (MQ=255)

                                                                                tttttttGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTT‑TTT‑C‑TTTTTTTTTCTTTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCATCATTTCTTATGTTTTTTGTTAAATCATGTCGTGGCATTCGTgtc                                                      <  2:2177164/140‑3 (MQ=255)

                                                                                tttttttGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTT‑TTT‑C‑TTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGc                                                      <  1:740549/140‑1 (MQ=255)

                                                                                tttttttGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTT‑TTT‑C‑TTTTTTTTGCTGTCCCGGGGGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGc                                                      <  1:2893421/140‑1 (MQ=255)

                                                                                 ttttttGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTT‑TTT‑C‑TTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGca                                                     <  2:2850492/140‑1 (MQ=255)

                                                                                 ttttttGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTT‑TTT‑C‑TTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGca                                                     <  1:1740807/140‑1 (MQ=255)

                                                                                 ttttttGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTT‑TTT‑C‑TTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGca                                                     <  1:354511/140‑1 (MQ=255)

                                                                                 ttttttGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTT‑TTT‑C‑TTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGca                                                     <  1:4072979/140‑1 (MQ=255)

                                                                                 ttttttGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTT‑TTT‑C‑TTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGca                                                     <  1:3302529/140‑1 (MQ=255)

                                                                                 ttttttGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTT‑TTT‑C‑TTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGca                                                     <  1:3142922/140‑1 (MQ=255)

                                                                                 ttttttGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTT‑TTT‑C‑TTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGca                                                     <  1:2810873/140‑1 (MQ=255)

                                                                                 ttttttGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTT‑TTT‑C‑TTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGca                                                     <  2:3994246/140‑1 (MQ=255)

                                                                                 ttttttGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTT‑TTT‑C‑TTTTTTTTGCTGTCCCGGGTGAAAAGTGAATGTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGca                                                     <  1:2260623/140‑1 (MQ=255)

                                                                                 ttttttGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTGTGACTT‑TTT‑C‑TTTTTTGTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGca                                                     <  2:3648813/140‑1 (MQ=255)

                                                                                   ttttGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTT‑TTT‑C‑TTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGcaca                                                   <  1:2560008/140‑1 (MQ=255)

                                                                                   ttttGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTT‑TTT‑C‑TTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGcaca                                                   <  2:498869/140‑1 (MQ=255)

                                                                                   ttttGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTT‑TTT‑C‑TTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGcaca                                                   <  1:1846854/140‑1 (MQ=255)

                                                                                   ttttGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTT‑TTT‑C‑TTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGcaca                                                   <  1:3034214/140‑1 (MQ=255)

                                                                                   ttttGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTT‑TTT‑C‑TTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGcaca                                                   <  1:1889215/140‑1 (MQ=255)

                                                                                    tttGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTT‑TTT‑C‑TTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTGTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGCACat                                                  <  2:264120/140‑1 (MQ=255)

                                                                                     ttGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTT‑TTT‑C‑TTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGCACata                                                 <  1:1456863/140‑1 (MQ=255)

                                                                                     ttGTGTGTTGTCTGGTAAAGCGGCCGCTTTATTTTTTGACTT‑TTT‑C‑TTTTGTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGCACata                                                 <  1:2098352/140‑1 (MQ=255)

                                                                                          ttttGTATGGTAAAGCGGCCGCTTTATTTTTTGACTT‑TTT‑C‑TTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGCACATAtatgt                                            <  1:650054/140‑1 (MQ=255)

                                                                                                  ggTAAAGCGGCCGCTTTATTTTTTGACTT‑TTT‑A‑TTTTTTTTTCTTTACCGGGTTAAAAGTGAAATTTCCCGTTTTCAGCCGTTAGTATGTTTTTTTGGCAATCCGGTCGTGGCATTACTGGCACATAAATTTATTGAttt                                    >  2:3754773/1‑139 (MQ=255)

                                                                                                    tAAAGCCGCAGATTAATTGTTGGACTT‑TGT‑C‑TTTTTATTGCTGTCCCGGGTGAAAAGTGAATGTTCACGTTTTCATCCGTTCTTCTGTTTTTTGGTCAATCCGGTCGTTTCATTAATTGAACATATATTTATGTATTggg                                  <  2:2506956/140‑1 (MQ=255)

                                                                                                     aaaGCGGCCGATTTATTTTTTGACTT‑TTT‑C‑TTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCAGTCCTGGCACATATATGTATGGATTGGGc                                 <  2:942112/140‑1 (MQ=255)

                                                                                                       aGCGGCCGCTTTATTTTTTGACTT‑TTT‑C‑TTTTTTTTTGTTTCCCGGGTGAAAAGTGAAATTTCCCCTTTTGATCCCTTAGTCTGTTTTTTTGGCAATACGGTCGTGGCATTGCTTGCACAAGAATGTATGGATTGGGCgg                               >  2:3699085/1‑140 (MQ=255)

                                                                                                       aGCGGCCGCTTTATTTTTTGACTT‑TTT‑C‑TTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATACGGTCGTGGCATTACTGGCACATATATGTATGGATTTGGCgg                               >  2:703805/1‑140 (MQ=255)

                                                                                                         cGGCCGCTTTATTTTTTGACTT‑TTT‑C‑TTTTTTTTTCTGTCCCGGTTGAAAAGTGAATTTTACCGTTTTGATCCGTGCTTCAGTTTTTTTGGCAATCCGGTCGTGGCATTCCTGGCACAAGAATGTAGGCTTGGGGCGGtt                             >  2:3472883/1‑140 (MQ=255)

                                                                                                              gCTTTATTTTTTGACTT‑TTT‑C‑TTTTTTTTGCTGTCCCGTGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATACGGTCGTGGCATTCCTGGCACATATATGTATGGATTGGGCGGTTTCgca                        >  2:4086642/1‑138 (MQ=255)

                                                                                                              gCTTTATTTTTTGACTT‑TTT‑C‑GTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTACCGTTTTCAGCCGTTCTGCTGTTTTTTGGGCAAACCGGTCGTGGCATTCCTGGCACATATATGTATGGATTTGGCGGTTTCGaa                        >  2:4240068/1‑140 (MQ=255)

                                                                                                                         tGACTT‑TTT‑C‑TTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGCACATATATGTATGGATTGGGCGGTTTCGAACCCGTTTcgcg             >  2:1206415/1‑140 (MQ=255)

                                                                                                                         tGACTT‑TTT‑C‑TTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTATTGTGTTTTTTGGGCAATCCGGTCGTGGCATTACTGGCACATATATGTATGGATTGGGCGGTTTCGAAGCAGTTTcgcg             >  2:3319119/1‑140 (MQ=255)

                                                                                                                         tGACTT‑TTT‑C‑TTTTTTTTGCTGTCCCGGGTGAAAAGTGAAGTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGCACATATATGTATGGATTGGGCGGTTTCGAAGCCGTTTcgcg             <  2:1390887/140‑1 (MQ=255)

                                                                                                                           agtt‑ttc‑c‑tttttGTGGCTGTCCCGGGTGAAAAGTGAATGTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGCACATATATGTATGGATTGGGCGGTTTCGAAGCCGTTTCGCGAt           <  2:1818632/138‑1 (MQ=255)

                                                                                                                           aCTT‑TTT‑C‑TTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGCACATATATGTATGGATTGGGCGGTTTCGAAGCCGTTTCGCGAt           <  2:1232857/140‑1 (MQ=255)

                                                                                                                           aCTT‑TTT‑C‑TTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGCACATATATGTATGGATTGGGCGGTTTCGAAGCCGTTTCGCGAt           <  1:1182424/140‑1 (MQ=255)

                                                                                                                           aCTT‑TTT‑C‑TTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGCACATATATGTATGGATTGGGCGGTTTCGAAGCCGTTTCGCGAt           <  1:1593100/140‑1 (MQ=255)

                                                                                                                           aCTT‑TTT‑C‑TTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGCACATATATGTATGGATTGGGCGGTTTCGAAGCCGTTTCGCGAt           <  2:3361633/140‑1 (MQ=255)

                                                                                                                           aCTT‑TTT‑C‑TTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGCACATATATGTATGGATTGGGCGGTTTCGAAGCCGTTTCGCGAt           <  2:153663/140‑1 (MQ=255)

                                                                                                                           aCTT‑TTT‑C‑TTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGCACATATATGTATGGATTGGGCGGTTTCGAAGCCGTTTCGCGAt           <  2:2710683/140‑1 (MQ=255)

                                                                                                                           aCTT‑TTT‑C‑TTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTGTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGCACATATATGTATGGATTGGGCGGTTTCGAAGCCGTTTCGCGAt           <  1:1107871/140‑1 (MQ=255)

                                                                                                                            ctt‑ttt‑c‑tttttTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGCACATATATGTATGGATTGGGCGGTTTCGAAGCCGTTTCGCGATc          <  2:3882559/140‑1 (MQ=255)

                                                                                                                            ctt‑ttt‑c‑tttttTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGCACATATATGTATGGATTGGGCGGTTTCGAAGCCGTTTCGCGATc          <  2:205394/140‑1 (MQ=255)

                                                                                                                             tt‑ttt‑c‑‑tttttTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGCACATATATGTATGGATTGGGCGGTTTCGAAGCCGTTTCGCGATCAg        <  1:2165468/140‑1 (MQ=255)

                                                                                                                              t‑ttt‑c‑tttttTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGCACATATATGTATGGATTGGGCGGTTTCGAAGCCGTTTCGCGATCAg        <  1:2665209/140‑1 (MQ=255)

                                                                                                                              t‑ttt‑c‑tttttTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGCACATATATGTATGGATTGGGCGGTTTCGAAGCCGTTTCGCGATCAg        <  1:1298591/140‑1 (MQ=255)

                                                                                                                                    c‑tttttTTTGCTGTCCCGGGTGAAAAGTTAATTGTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGCACATATATGTATGGATTGGGCGGTTTCGAAGCCGTTTCGCGATCAGGGTa    <  2:3597670/140‑1 (MQ=255)

                                                                                                                                    c‑tttttTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGCACATATATGTATGGATTGGGCGGTTTCGAAGCCGTTTCGCGATCAGGGTa    <  1:3945566/140‑1 (MQ=255)

                                                                                                                                     tttttttttGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGCACATATATGTATGGATTGGGCGGTTTCGAAGCCGTTTCGCGATCAGGGTa    <  2:4025155/139‑1 (MQ=255)

                                                                                                                                       tttttttGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGCACATATATGTATGGATTGGGCGGTTTCGAAGCCGTTTCGCGATCAGGGTatc  <  2:726581/140‑1 (MQ=255)

                                                                                                                                             tGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGCACata                                                 >  1:3627929/1‑87 (MQ=255)

CATTTACAAAATTCGGCTGTATCAGTCGAAAAAGGGACAGTCTGCAAAATCATAAAAAGACTCAGCTGAGAAGCTGAGTCTTTTTTTGTGTTTTGTATGGTAAAGCGGCCGCTTTATTTTTTGACTT‑TTT‑C‑TTTTTTTTGCTGTCCCGGGTGAAAAGTGAATTTTCCCGTTTTCAGCCGTTCTTCTGTTTTTTGGGCAATCCGGTCGTGGCATTCCTGGCACATATATGTATGGATTGGGCGGTTTCGAAGCCGTTTCGCGATCAGGGTATC  >  NZ_CP034943/1514742‑1515013

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`