BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	3,746,962	C→A	6.7%	D36Y (GAT→TAT)	EO946_RS19275 ←	galactokinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	3,746,962	0	C	A	6.7%	657.0 / 2.4	256	D36Y (GAT→TAT)	EO946_RS19275	galactokinase
Reads supporting (aligned to +/- strand): ref base C (118/105); new base A (27/3); total (148/108)
Fisher's exact test for biased strand distribution p-value = 5.77e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 260 total aligned reads displayed.

TCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAGCGCGCACGGAAACACAT‑GCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCG‑AAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATCCCCTTTAGCTCTTCCCGCATGTGCAATCACCTTTCATTGTTTTATTTTTTTAT  >  NZ_CP034943/3746823‑3747100

tcgtcTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAGCGCGCACGGAAACACAT‑GCCCGCCGTTATAATc                                                                                                                                             <  1:2129930/140‑1 (MQ=255)

   tcTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAGCGCGCACGGAAACACAT‑GCCCGCCGTTATAATCTGt                                                                                                                                          <  1:1008818/140‑1 (MQ=255)

     tAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTATGCAACAGCAGCATATGTAACCATTGTCAGCCCCCACGGAAACACAA‑TCCCCCCGTTAAAAAATGTAt                                                                                                                                        >  2:4163105/1‑140 (MQ=255)

            cATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTGGGCAACACCAGCATAAGTAACCAAGGTCAGCGCGAACGGAAACACAT‑TCCCCCCGTTATAAAAAGTATGTtcaac                                                                                                                                 >  2:346908/1‑137 (MQ=255)

            cATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCAAATGTACCCATTTTCAGCGCGCACGGAAACACAA‑GCCCCCCGTTATAATCTGTATGTtatca                                                                                                                                 >  2:2814107/1‑139 (MQ=255)

            cATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAAAAGCAGCATATGTACCCAATGTAAGCGCGCACGGAAACACAA‑GCCCCCCCTTATAAAATGTATGTTATcc                                                                                                                                 >  2:2730617/1‑140 (MQ=255)

            cATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTATGCAACAGCAGCAAATTTAACCATTTTCAGCGCGCACCGAAAAACAT‑GCCCGCCGTTATAATATGTATGttatac                                                                                                                                 >  2:1575078/1‑136 (MQ=255)

            cATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTATGCAACACCAGCAAATGTACCCATTGTCAGCGCGCACGGAAAAACAT‑GCCCCCCGTTATAAAATTTATGTTATcc                                                                                                                                 >  2:541194/1‑140 (MQ=255)

            cATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTGTCTTTCTGCAACAGCAGCATATGTACCCAATGTCAGCGCGCACGGAAACACAT‑GCCCGCCGTTATAAACTGTATGTTCTcc                                                                                                                                 >  2:966505/1‑140 (MQ=255)

            cATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTGTCTTTATGCAAAAGCACCATAAGTAACCATTGTCAGCCCGCCCCGAAACAAAA‑GCCCCCCCTTAAAATAGGTATTTTATcc                                                                                                                                 >  1:786286/1‑140 (MQ=255)

               tCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAGCGCGCACGGAAACACAT‑GCCCGCCGTTATAATCTGTATGTTCTCCAAt                                                                                                                              <  1:1279036/140‑1 (MQ=255)

                      tCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTGTTTCTGCAACACCACCAAATTTACACATTGTCAGACCGCACCGAAAAAAAT‑TCACCCCGGTATAAAAATTATGTTATCCAAACACAtta                                                                                                                       >  1:2480193/1‑139 (MQ=255)

                      tCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCATTCTGCAACACCACCATAAGTACCCATTGTCAGCGCGCACGGAAACACAA‑GCCCCCCCTTATAATATGTATGTTCTCCAAACACAtta                                                                                                                       >  2:1528679/1‑139 (MQ=255)

                       cccTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAGCGCGCACGGAAACACAT‑GCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCa                                                                                                                      <  2:3026753/140‑1 (MQ=255)

                       cccTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAGCGCGCACGGAAACACAT‑GCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCa                                                                                                                      <  2:1210484/140‑1 (MQ=255)

                          tACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACACCCCCATAATTACCCATTGTCAGCGCGCACCGAAACAAAA‑GCCCGCCGTTATAAACTGGATGTTCTCCAATCAGATTCActc                                                                                                                   >  2:1773891/1‑140 (MQ=255)

                           aCGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTTTGCAACAGCAGCATAAGTACACCTTGTGAGCGCGCCACGAAACAAAT‑GCCCGCCCTTATAAAATGGATTTTCTCCAATCATATTCAcact                                                                                                                  >  2:348475/1‑140 (MQ=255)

                           aCGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCAATGTCAGCCCGCACCGAAACACAT‑GCCCGCCGGTATAATATGTATGTTCTCCAATCACATTAAcact                                                                                                                  >  2:1804865/1‑140 (MQ=255)

                           aCGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTATTTAATAAACAACAGCAAATGTACCCATAGTCAGCGCGC‑CAGGAACAAATAGACCGCCGAAATAAACGGTCTGTTGTCAAATCACTATCActct                                                                                                                  >  2:3358712/1‑140 (MQ=255)

                                 ttAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTGTGATTGTTCAACAGCAGCCTATGTACCCATTGTAAGCGCGCACGGAAACACAA‑GCCCGCCGGTATAATCGGGATGTTGTCCAATGAGATTCACTATTCCAgg                                                                                                            >  2:1136637/1‑140 (MQ=255)

                                  tAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCACCAGAACCATATGTAACCAATGTCACCGCGCACGGAAACACAA‑GCCCGCCGTTATAATATGTATTTTCTCCAAAAAGATTAACTCTTCCAGGt                                                                                                           >  2:1880614/1‑140 (MQ=255)

                                  tAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTGTCTTTCTGCAACAGCAGCATATGTAACCATTGTCATCGCGCACGGAAACACAT‑GCCCGCCGTTATAATCTGTATTTTCTCAAAACAGATTCAATCTTACAGGt                                                                                                           >  2:4109664/1‑140 (MQ=255)

                                         gTCTGAATACATCCGGACAACCCCGTCGTGTCTATATTGAAAAGGAGCATATTTACACAATGTAAGCGCGCAACGAAACACAT‑GGCCGACGTTTTAATCTGGATGTTGACCAATAAGATTGAGTCTTGAAGGTTGG‑aaat                                                                                                   >  2:164053/1‑139 (MQ=255)

                                          tCTGAATACATCCGGACAACCCCGTCGTGTCTTTCTGCAAAACCACCATATGTACCCAGTTTCACCGCGCAAGGAAACACAA‑GCCCGCCGTTATAATATGTATGTTCTACCATCAGATTAACTATTACAGGTGCG‑aaaaa                                                                                                  >  2:629089/1‑140 (MQ=255)

                                           cggAATACATCCTGAAAACCCCGTCGTTTCTTTCGGCAACAGCAGCATATGTACCCATTGTCAGCGCGCACGGAAACACAT‑GCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCG‑aaaaaa                                                                                                 <  2:3297990/138‑1 (MQ=255)

                                           cTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAGCGCGCACGGAAACACAT‑GCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCG‑aaaaaa                                                                                                 <  1:2037386/140‑1 (MQ=255)

                                           cTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAGCGCGCACGGAAACACAT‑GCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCG‑aaaaaa                                                                                                 <  1:4107871/140‑1 (MQ=255)

                                                 aCATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAGCGCGCACGGAAACACAT‑GCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCG‑AAAAAAAACCGt                                                                                           <  1:3537093/140‑1 (MQ=255)

                                                    tCCGGACAACCCCGTCGTTTGTTTCTGCAACAGCAGCATATGTACCCATTGTCAGCGCGCACGGAAACACAT‑GCCCGCCGTTAAAATCTGTATGTTCGACAATCAGATTCACTCTTACAGGTGCG‑AAAAAAAACCGTAAc                                                                                        >  2:4302184/1‑140 (MQ=255)

                                                    tCCGGACAACCCCGTCGTTTGTTTAGTCAACAGCAGCAGAGGTACCCATTGTCACCGCGCCCGGAAACAAAT‑GCCCCCCGTTATAATATGTATGGTGTACAAGCAGATTCAACAGTCCAGGTGCG‑AAAAAAAAACGTAAc                                                                                        >  2:262189/1‑140 (MQ=255)

                                                      cGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAGCGCGCACGGAAACACAT‑GCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCG‑AAAAAAAACCGTAAccc                                                                                      <  2:3815237/140‑1 (MQ=255)

                                                        gACAACCCCGTCGTGTCTTTCTTCAACAGCAGCAAATGTACCCATTGTCAGCGCGCACGGAAACAAAT‑GCCCGCCCTTATAATCTGTATGTTCTCCAATAAGATTAACTCTTACATGTGCG‑AAAAAAAAACGTAAcaccg                                                                                    >  2:2456455/1‑139 (MQ=255)

                                                             ccccGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAGCGCGCACGGAAACACAT‑GCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCG‑AAAAAAAACCGTAACCCCTCtttt                                                                               <  2:3175897/140‑1 (MQ=255)

                                                             ccccGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAGCGCGCACGGAAACACAT‑GCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCG‑AAAAAAAACCGTAACCCCTCtttt                                                                               <  2:1457790/140‑1 (MQ=255)

                                                                cgtcgtTTCTTTCTGCAACCGCAGCATATGTACCCATTGTCAGCGCGCACGGAAACACAT‑GCCCGCCGTTATAATCGGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCG‑AAAAAAAACCGTACCCCCTCTTTTtct                                                                            <  1:1804865/140‑1 (MQ=255)

                                                                cgtcgtTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAGCGCGCACGGAAAGACAT‑GCCCGCCGTTATAGTCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCG‑AAAAAAAACCGTAACCCCTCTTTTtct                                                                            <  1:2688433/140‑1 (MQ=255)

                                                                cgtcgtTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAGCGCGCACGGAAACACAT‑GCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCG‑AAAAAAAACCGTAACCCCTCTTTTtct                                                                            <  2:3008629/140‑1 (MQ=255)

                                                                cgtcgtTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAGCGCGCACGGAAACACAT‑GCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCG‑AAAAAAAACCGTAACCCCTCTTTTtct                                                                            <  1:4163105/140‑1 (MQ=255)

                                                                      ttctttctGCAACAGCAGCATATGTACCCATTGTCAGCGCGCACGGAAACACAT‑GCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCG‑AAAAAAAACCGTAACCCCTCTTTTTCTCCGAAt                                                                      <  2:4322101/140‑1 (MQ=255)

                                                                       tctttctGCAACAGCAGCATATGTACCCATTGTCAGCGCGCACGGAAACACAT‑GCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCG‑AAAAAAAACCGTAACCCCTCTTTTTCTCCGAATa                                                                     <  1:953468/140‑1 (MQ=255)

                                                                       tctttctGCAACAGCAGCATATGTACCCATTGTCAGCGCGCACGGAAACACAT‑GCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCG‑AAAAAAAACCGTAACCCCTCTTTTTCTCCGAATa                                                                     <  2:3742222/140‑1 (MQ=255)

                                                                       tctttctGCAACAGCAGCATATGTACCCATTGTCAGCGCGCACGGAAACACAT‑GCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCG‑AAAAAAAACCGTAACCCCTCTTTTTCTCCGAATa                                                                     <  1:3331212/140‑1 (MQ=255)

                                                                       tctttctGCAACAGCAGCATATGTACCCATTGTCAGCGCGCACGGAAACACAT‑GCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCG‑AAAAAAAACCGTAACCCCTCTTTTTCTCCGAATa                                                                     <  2:3516709/140‑1 (MQ=255)

                                                                             tGCAACAGCAGCATATGTACCCATTGTCAGCGCGCACTGAAACACTT‑GCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCG‑AAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTg                                                               <  2:4132509/140‑1 (MQ=255)

                                                                             tGCAACAGCAGCATATGTACCCATTGTCAGCGCGCACGGAAACACAT‑GCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCG‑AAAAAAAACCGTAACCCCCCTTTTTCTCCGAATACACTTg                                                               <  2:1099681/140‑1 (MQ=255)

                                                                                   agcagcATATGTACCCATTGTCAGCGCGCACGGAAACACAT‑GCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCG‑AAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAata                                                         <  2:506292/140‑1 (MQ=255)

                                                                                     cagcaTATGTACCCATTGTCAGCGCGCACGGAAACACAT‑GCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCG‑AAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATc                                                       <  1:3388721/140‑1 (MQ=255)

                                                                                     cagcaTATGTACCCATTGTCAGCGCGCACGGAAACACAT‑GCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCG‑AAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATc                                                       >  2:292840/1‑140 (MQ=255)

                                                                                     cagcaTATGTACCCATTGTCAGCGCGCACGGAAACACAT‑GCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCG‑AAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATc                                                       <  1:2912170/140‑1 (MQ=255)

                                                                                     cagcaTATGTACCCATTGTCAGCGCGCACGGAAACACAT‑GCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCG‑AAAAAAAAACGTTAACCCTCTTTTTCTCCGAAAACACATGCAAatata                                                       >  2:2105344/1‑139 (MQ=255)

                                                                                         atatGTACCCATTGTCAGCGCGCACGGAAACACAT‑GCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCG‑AAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATCCCCt                                                   <  1:1575078/140‑1 (MQ=255)

                                                                                             gTACCCATTGTCAGCTCGCACGGAAACACAT‑GCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCG‑AAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATCCCCTTTAg                                               <  1:3161293/140‑1 (MQ=255)

                                                                                             gTACCCATTGTCAGCGCGCACGGAAACACAT‑GCCCGCCGTTATAATCTGTATGTtctc                                                                                                                                  <  2:359349/58‑1 (MQ=255)

                                                                                             gTACCCATTGTCAGCGCGCACGGAAACACAT‑GCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCG‑AAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATCCCCTTTAg                                               <  2:2329810/140‑1 (MQ=255)

                                                                                             gTACCCATTGTCAGCGCGCACGGAAACACAT‑GCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCG‑AAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATCCCCTTTAg                                               <  1:1880614/140‑1 (MQ=255)

                                                                                             gTACCCATTGTCAGCGCGCACGGAAACACAT‑GCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCG‑AAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATCCCCTTTAg                                               <  2:1168290/140‑1 (MQ=255)

                                                                                             gTACCCATTGTCAGCGCGCACGGAAACACAT‑GCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCG‑AAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATCCCCTTTAg                                               >  1:375655/1‑140 (MQ=255)

                                                                                               aCCCATTGTCAGCGCGCACGGAAACACAT‑GCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCG‑AAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATCCCCTTTAGct                                             >  2:362530/1‑140 (MQ=255)

                                                                                               aCCCATTGTCAGCGCGCACGGAAACACAT‑GCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCG‑AAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATCCCCTTTAGct                                             >  2:4013439/1‑140 (MQ=255)

                                                                                                cccATTGTCAGCGCGCACGGAAACACAT‑GCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCG‑AAAAAAAACCGTAAccc                                                                                      >  2:1360436/1‑98 (MQ=255)

                                                                                                cccATTGTCAGCGCGCACGGAAACACAT‑GCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCG‑AAAAAAAACCGTAAccc                                                                                      <  1:1360436/98‑1 (MQ=255)

                                                                                                  cATTGTCAGCGCGCACGGAAACACAT‑GCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCG‑AAAAAAAACCGTAACCCCTCtttt                                                                               <  2:3574784/103‑1 (MQ=255)

                                                                                                  cATTGTCAGCGCGCACGGAAACACAT‑GCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCG‑AAAAAAAACCGTAACCCCTCtttt                                                                               >  1:3574784/1‑103 (MQ=255)

                                                                                                  cATTGTCAGCGCGCACGGAAACACAT‑GCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCG‑AAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATCCCCTTTAGCTCtt                                          >  1:2322422/1‑140 (MQ=255)

                                                                                                  cATTGTCAGCGCGCACGGAAACACAT‑GCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCG‑AAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATCCCCTTTAGCTCtt                                          >  1:118174/1‑140 (MQ=255)

                                                                                                  cATTGTCAGCGCGCACGGAAACACAT‑GCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCG‑AAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATCCCCTTTAGCTCtt                                          >  1:3630269/1‑140 (MQ=255)

                                                                                                  cATTGTCAGCGCGCACGGAAACACAT‑GCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCG‑AAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATCCCCTTTAGCTCtt                                          >  1:746305/1‑140 (MQ=255)

                                                                                                  cATTGTCAGCGCGCACGGAAACACAT‑GCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCG‑AAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATCCCCTTTAGCTCtt                                          >  2:1767355/1‑140 (MQ=255)

                                                                                                  cATTGTCAGCGCGCACGGAAACACAT‑GCCCGCCGTTATAATCTGTATGTTCTCCAAGCAGATTCACTCTTCCAGGTGCG‑AAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATCCCCTTTAGCTCtt                                          >  2:765668/1‑140 (MQ=255)

                                                                                                   aTTGTCAGCGCGCACGGAAACACAT‑GCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAAACCGTACCCCCGCTTTTTCTCCGAATACACTTGCAAATATCCCCTTTAGCTGtt                                          >  1:1974931/1‑140 (MQ=255)

                                                                                                   aTTGTCAGCGCGCACGGAAACACAT‑GCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCG‑AAAAACAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATCGCCTTTAGATCTTc                                         >  2:2077751/1‑140 (MQ=255)

                                                                                                    ttGTCAGCGCGCACGCAAACACAT‑GCCCGCCGTTATAATCTGTATGTACTCCAATCAGATTAACTCTTCCAGGTGCG‑AAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATCACAGTTAGCTCTTcc                                        >  1:992466/1‑140 (MQ=255)

                                                                                                     tGTCAGCGCGCACGGAAACACAT‑GCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCG‑AAATAAATCCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATCCCCTTTAGCTCTTccc                                       >  2:2000331/1‑140 (MQ=255)

                                                                                                     tGTCAGCGCGCACGGAAACACAT‑GCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCG‑AAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATCCCCTTTAGCTCTTccc                                       >  2:2779789/1‑140 (MQ=255)

                                                                                                     tGTCAGCGCGCACGGAAACACAT‑GCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCG‑AAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATCCCCTTTAGCTCTTccc                                       >  2:883547/1‑140 (MQ=255)

                                                                                                     tGTCAGAGCGCACTGAAACACAT‑GCCCGCCGTTATAATATGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCG‑AAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATCCCCTTTAGCTCTTccc                                       >  2:914850/1‑140 (MQ=255)

                                                                                                      gTCAGCGCGCACGGAAACACAT‑GCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCG‑AAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATCCCCTTTAGCTCTTCCCg                                      >  2:831726/1‑140 (MQ=255)

                                                                                                        cAGCGCGCACGGAAACACAT‑GCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCG‑AAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATCCCCTTTAGCTCTTCCCGCa                                    <  1:3502480/140‑1 (MQ=255)

                                                                                                        cAGCGCGCACGGAAACACAT‑GCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCG‑AAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATCCCCTTTAGCTCTTCCCGCa                                    <  1:2807614/140‑1 (MQ=255)

                                                                                                        cAGCGCGCACGGAAACACAT‑GCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCG‑AAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATCCCCTTTAGCTCTTCCCGCa                                    >  2:3084664/1‑140 (MQ=255)

                                                                                                         aGCGCGCACGGAAACACAT‑GCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCG‑AAAAAAAACCGTACCCCCACTTTTTCTCCGAATACACTAGCAAATATCCCCTTTAGCTCTTCCGGCAt                                   >  2:2551050/1‑140 (MQ=255)

                                                                                                           cgcgcACGGAAACACAT‑GCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCG‑AAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATCCCCTTTAGCTCTTCCCGCAtgt                                 >  1:2188414/1‑140 (MQ=255)

                                                                                                            gcgcACGGAAACACAT‑GCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCG‑AAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATCCCCTTTAGCTCTTCCCGCAtgtg                                <  2:1242110/140‑1 (MQ=255)

                                                                                                             cgcACGGAAACACAT‑GCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCG‑AAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATCCCCTTTAGCTCTTCCCGCATGTGc                               >  1:2179081/1‑140 (MQ=255)

                                                                                                             cgcACGGAAACACAT‑GCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCG‑AAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATCCCCTTTAGCTCTTCCCGCATGTGc                               >  1:432550/1‑140 (MQ=255)

                                                                                                                          aT‑GCCCGCCGTTATAATCTGTATGTTCTCCAATCCGATTCACTCTTCCAGGTGCG‑AAAAAAAACCGTAACGCCTCTTTTTCGCCGAATACACTTGCAAATATCCCCTTTAGCTCTTCCCGCATGTGCAATCACCTTTcgt                  >  1:3204557/1‑138 (MQ=255)

                                                                                                                          aT‑GCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCG‑AAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTg                                                               >  1:2983338/1‑95 (MQ=255)

                                                                                                                          aT‑GCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCG‑AAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATCTCCCCTTTAGCTCTTCCCGCATGTGCCATCACCttgcct                  >  1:2723370/1‑136 (MQ=255)

                                                                                                                          aT‑GCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCG‑AAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATc                                                       <  1:1771326/103‑1 (MQ=255)

                                                                                                                          aT‑GCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCG‑AAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATCCCCTTTAGCTCTTCCCGCATGTGCACTCACCTTTCAt                  >  1:556182/1‑140 (MQ=255)

                                                                                                                          aT‑GCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCG‑AAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATCCCCTTTAGCTCTTCCCGCATGTGCAATCACCTTTCAt                  >  1:3799367/1‑140 (MQ=255)

                                                                                                                             gCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCG‑AAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATCCCCTTTAGCTCTTCCCGCATGTGCAATCACCTTTGATTg                >  2:2808476/1‑140 (MQ=255)

                                                                                                                             gCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCG‑AAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATCCCCTTTAGCTCTTCCCGCATGTGCAATCACCTTTCATTg                >  1:1936454/1‑140 (MQ=255)

                                                                                                                               ccgccgTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCG‑AAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATCCCCTTTAGCTCTTCCCGCATGTGCTATCACCTTTCATTGtt              <  1:2456455/140‑1 (MQ=255)

                                                                                                                               ccgccgTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCG‑AAAAAAAACCGTAAACCCTCTTTTTATCCGAATACACTTGCAAATATCCCCTTTAGCTCTTCCCTCATGTGCAATCACCTTTCATTGtt              <  1:3134219/140‑1 (MQ=255)

                                                                                                                                 gccgTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCG‑AAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATCCCCTTTAGCTATTCCCGCATGTTCAATCACCTTTCATTGtttt            >  2:3220994/1‑140 (MQ=255)

                                                                                                                                   cgTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCG‑AAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATCCCCTTTAGCTCTTCCCGCATGTGCAATCACCTTTCATTGttttat          >  2:4208405/1‑140 (MQ=255)

                                                                                                                                           tCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCG‑AAAAAAAACCGTAACCCCTCTTTTTCTCCGAATCCACTTGCAAATATCCCCTTTAGCTCTTCCCGCATGTGCAATCACCTTTCATTGttttatttttttat  <  2:2129093/140‑1 (MQ=255)

                                                                                                                                           tCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCG‑AAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATCCCCTTTAGCTCTTCCCGCATGTGCAATCACCTTTCATTGttttatttttttat  <  1:3656586/140‑1 (MQ=255)

                                                                                                                                           tCTGTAAGTTCGCCAATCCGATTCACTCTGCCAGGTGCT‑AAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATCCCCTTTAGCTCTTCCCGCATGTGCAATCACCTTTCATTGttttatttttttat  <  2:2197392/140‑1 (MQ=255)

TCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGTACCCATTGTCAGCGCGCACGGAAACACAT‑GCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCG‑AAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATCCCCTTTAGCTCTTCCCGCATGTGCAATCACCTTTCATTGTTTTATTTTTTTAT  >  NZ_CP034943/3746823‑3747100

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`