BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	2,301,730	C→G	5.3%	G157G (GGG→GGC)	lepB ←	signal peptidase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	2,301,730	0	C	G	5.3%	244.9 / 5.2	75	G157G (GGG→GGC)	lepB	signal peptidase I
Reads supporting (aligned to +/- strand): ref base C (35/35); new base G (2/2); total (37/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.46e-01

ATGAACGAAAAAAGAAATAAAAAAAGCAGCCTCCTGTTCAGGAAACTGCTTGATCCTAATTTGTTTTGCGCATGTCGTTAAACGGGTAGAAAACAAACTTAGACTTGCCCGCAATTTG‑TTTTTTCGTGAAGAGGCCAAGCCCGTTTCGGCTGTCCATTGAGTTCCGCCGGTTGTCACCCATCACAAAATACTTGTCATCAGGCACTTTGATCGGGCCGAAATCATCGGTTAAATTGTCAAAACCTTCATTTTTGGCTTTCTTTTTATATGCCTTCAAA  >  NZ_CP034943/2301591‑2301868

aTGAACGAAAAAAGAAATAAAAAAAGCAGCCTCCTGTTCAGGAAACTGCTTGATCCTAATTTGTTTTGCGCATGTCGTTAAACGGGTAGAAAACAAACTTAGACTTGCCCGCAATTTG‑TTTTTTCGTGAAGAGGCCAAGc                                                                                                                                            >  2:178689/1‑140 (MQ=255)

aTGAACGAAAAAAGAAATAAAAAAAGCAGCCTCCTGTTCAGGAAACTGCTTGATCCTAATTTGTTTTGCGCATGTCGTTAAACGGGTAGAAAACAAACTTAGACTTGCCCGCAATTTG‑TTTTTTCGTGAAGAGGCCAAGc                                                                                                                                            >  1:2026214/1‑140 (MQ=255)

aTGAACGAAAAAAGAAATAAAAAAAGCAGCCTCCTGTTCAGGAAACTGCTTGATCCTAATTTGTTTTGCGCATGTCGTTAAACGGGTAGAAAACAAACTTAGACTTGCCCGCAATTTG‑TTTTTTCGTGAAGAGGCCAAGc                                                                                                                                            >  1:220174/1‑140 (MQ=255)

aTGAACGAAAAAAGAAATAAAAAAAGCAGCCTCCTGTTCAGGAAACTGCTTGATCCTAATTTGTTTTGCGCATGTCGTTAAACGGGTAGAAAACAAACTTAGACTTGCCCGCAATTTG‑TTTTTTCGTGAAGAGGCCAAGc                                                                                                                                            >  2:32016/1‑140 (MQ=255)

aTGAACGAAAAAAGAAATAAAAAAAGCAGCCTCCTGTTCAGGAAACTGCTTGATCCTAATTTGTTTTGCGCATGTCGTTAAACGGGTAGAAAACAAACTTAGACTTGCCCGCAATTTG‑TTTTTTCGTGAAGAGGCCAAGc                                                                                                                                            >  1:259021/1‑140 (MQ=255)

aTGAACGAAAAAAGAAATAAAAAAAGCAGACTCCTGTTAAGGAAACTCCTAGAACCTAATTTGTTTTGCGCATGTCGTTAAACGGGTAGAAAACAAACTTAGACTTGCCCTCAATATG‑ATTTTTAGTGAAGAGGCCAAGc                                                                                                                                            >  2:1569937/1‑140 (MQ=255)

 tGAACGAAAAAAGAAATAAAAAAAGCAGCCTCCTGTTCAGGAAACTGCTTGATCCTAATTTTTTTTGCGCATGTCGTTAAACGGGTAGAAAACAAACTTATACTTGCCCGCAATTTG‑TTTTTTCGTGAAGAGGCAAATcc                                                                                                                                           >  2:2082894/1‑140 (MQ=255)

      gAAAAAAGAAATAAAAAAAGCAGCCTCCTGTTCAGGAAACTGCTTGATCCTAATTTGTTTTGCGCATGTCGTTAAACGGGTAGAAAACAAACTTAGACTTGCCCGCAATTTG‑TTTTTTCGTGAAGAGGCCAAGCCCGttt                                                                                                                                      >  1:1221576/1‑140 (MQ=255)

      gAAAAAAGAAATAAAAAAAGCAGCCTCCTGTTCAGGAAACTGCTTGATCCTAATTTGTTTTGCGCATGTCGTTAAACGGGTAGAAAACAAACTTAGACTTGCCCGCAATTTG‑TTTTTTCGTGAAGAGGCCAAGCCCGttt                                                                                                                                      >  1:405358/1‑140 (MQ=255)

        aaaaaGAAATAAAAAAAGCAGCCTCCTGTTCAGGAAACTGCTTGATCCTAATTTGTTTTGCGCATGTCGTTAAACGGGTAGAAAACAAACTTAGACTTGCCCGCAATTTG‑TTTTTTCGTGAAGAGGCCAAGCCCGTTTCg                                                                                                                                    >  2:740004/1‑140 (MQ=255)

           aaGAAATAAAAAAAGCAGCCTCCTGTTCAGGAAACTGCTTGATCCTAATTTGTTTTGCGCATGTCGTTAAACGGGTAGAAAACAAACTTAGACTTGCCCGCAATTTG‑TTTTTTCGTGAAGAGGCCAAGCCCGTTTCGGCt                                                                                                                                 <  1:621145/140‑1 (MQ=255)

           aaGAAATAAAAAAAGCAGCCTCCTGTTCAGGAAACTGCTTGATCCTAATTTGTTTTGCGCATGTCGTTAAACGGGTAGAAAACAAACTTAGACTTGCCCGCAATTTG‑TTTTTTCGTGAAGAGGCCAAGCCCGTTTCGGCt                                                                                                                                 <  1:621899/140‑1 (MQ=255)

           aaGAAATAAAAAAAGCAGCCTCCTGTTCAGGAAACTGCTTGATCCTAATTTGTTTTGCGCATGTCGTTAAACGGGTAGAAAACAAACTTAGACTTGCCCGCAATTTG‑TTTTTTCGTGAAGAGGCCAAGCCCGTTTCGGCt                                                                                                                                 <  2:2283282/140‑1 (MQ=255)

           aaGAAATAAAAAAAGCAGCCTCCTGTTCAGGAAACTGCTTGATCCTAATTTGTTTTGCGCATGTCGTTAAACGGGTAGAAAACAAACTTAGACTTGCCCGCAATTTG‑TTTTTTCGTGAAGAGGCCAAGCCCGTTTCGGCt                                                                                                                                 <  2:825584/140‑1 (MQ=255)

           aaGAAATAAAAAAAGCAGCCTCCTGTTCAGGAAACTGCTTGATCCTAATTTGTTTTGCGCATGTCGTTAAACGGGTAGAAAACAAACTTAGACTTGCCCGCAATTTG‑TTTTTTCGTGAAGAGGCCAAGCCCGTTTCGGCt                                                                                                                                 <  2:1291922/140‑1 (MQ=255)

           aaGAAATAAAAAAAGCAGCCTCCTGTTCAGGAAACTGCTTGATCCTAATTTGTTTTGCGCATGTCGTTAAACGGGTAGAAAACAAACTTAGACTTGCCCGCAATTTG‑TTTTTTCGTGAAGAGGCCAAGCCCGTTTCGGCt                                                                                                                                 <  2:407459/140‑1 (MQ=255)

           aaGAAATAAAAAAAGCAGCCTCCTGTTCAGGAAACTGCTTGATCCTAATTTGTTTTGCGCATGTCGTTAAACGGGTAGAAAACAAACTTAGACTTGCCCGCAATTTG‑TTTTTTCGTGAAGAGGCCAAGCCCGTTTCGGCt                                                                                                                                 <  1:1947360/140‑1 (MQ=255)

           aaGAAATAAAAAAAGCAGCCTCCTGTTCAGGAAACTGCTTGATCCTAATTTGTTTTGCGCATGTCGTTAAACGGGTAGAAAACAAACTTAGACTTGCCCGCAATTTG‑TTTTTTCGTGAAGAGGCCAAGCCCGTTTCGGCt                                                                                                                                 <  2:894981/140‑1 (MQ=255)

                      aaaGCAGCCTCCTGTTCAGGAAACTGCTTGATCCTAATTTGTTTTGCGCATGTCTTTAAACGGGTAGAAAACAAACTTAGACTTGCCCGCAATTTG‑TTTTTTCGTGAAGATTCAATTACAGTTTCGGCTGTCCATTgttt                                                                                                                      <  2:1491014/140‑4 (MQ=255)

                        agcagcCTCCTGTTCAGGAAACTGCTTGATCCTAATTTGTTTTGCGCATGTCGTTAAACGGGTAGAAAACAAACTTAGACTTGCCCGCAATTTG‑TTTTTTCGTGAAGAGGCCAAGCCCGTTTCGGCTGTCCATTGAGTTc                                                                                                                    >  2:754933/1‑140 (MQ=255)

                           agcCTCCTGTTCAGGAAACTGCTTGATCCTACTTTGTTTTGCGCAGGTCGTTAAACGGGTAGAAAACAAACTTAGACTTGCCCGCAATTTG‑TTTTTTCGTGAAGAGGCCAAGCCCGTTTCGGCTGTCCATTGAGTTccgc                                                                                                                 <  1:1039518/140‑1 (MQ=255)

                           agcCTCCTGTTCAGGAAACTGCTTGATCCTAATTTGTTTTGCGCATGTCGTTAAACGGGTAGAAAACAAACTTAGACTTGCCCGCAATTTG‑TTTTTTCGTGAAGAGGCCAAGCCCGTTTCGGCTGTCCATTGAGTTccgc                                                                                                                 <  1:2194278/140‑1 (MQ=255)

                           agcCTCCTGTTCAGGAAACTGCTTGATCCTAATTTGTTTTGCGCATGTCGTTAAACGGGTAGAAAACAAACTTAGACTTGCCCGCAATTTG‑TTTTTTCGTGAAGAGGCCAAGCCCGTTTCGGCTGTCCATTGAGTTccgc                                                                                                                 <  2:220174/140‑1 (MQ=255)

                           agcCTCCTGTTCAGGAAACTGCTTGATCCTAATTTGTTTTGCGCATGTCGTTAAACGGGTAGAAAACAAACTTAGACTTGCCCGCAATTTG‑TTTTTTCGTGAAGAGGCCAAGCCCGTTTCGGCTGTCCATTGAGTTccgc                                                                                                                 <  2:259021/140‑1 (MQ=255)

                           agcCTCCTGTTCAGGAAACTGCTTGATCCTAATTTGTTTTGCGCATGTCGTTAAACGGGTAGAAAACAAACTTAGACTTGCCCGCAATTTG‑TTTTTTCGTGAAGAGGCCAAGCCCGTTTCGGCTGTCCATTGAGTTccgc                                                                                                                 <  1:800325/140‑1 (MQ=255)

                           agcCTCCTGTTCAGGAAACTGCTTGATCCTAATTTGTTTTGCGCATGTCGTTAAACGGGGAGAAAACAAACTTAGACTTGCCCGCAATTTG‑TTTTTTCGTGAAGAGGCCAAGCCCGTTTCGGCTGTCCATTGAGTTccgc                                                                                                                 <  1:14442/140‑1 (MQ=255)

                                    ttCAGGAAACTGCTTGATCCTAATTTGTTTTGCGCATGTCGTTAAACGGGTAGAAAACAAACTTAGACTTGCCCGCAATTTG‑TTTTTTCGTGAAGAGGCCAAGCCCGTTTCGGCTGTCCATTGAGTTCCGCCGGTTGTCa                                                                                                        <  2:1075323/140‑1 (MQ=255)

                                        ggAAACTGCTTGATCCTAATTTGTTTTGCGCATGTCGTTAAACGGGTAGAAAACAAACTTAGACTTGCCCGCAATTTG‑TTTTTTCGTGAAGAGGCCAAGCCCGTTTCGGCTGTCCATTGAGTTCCGCCGGTTGTCACCCa                                                                                                    <  1:701589/140‑1 (MQ=255)

                                        ggAAACTGCTTGATCCTAATTTGTTTTGCGCATGTCGTTAAACGGGTAGAAAACAAACTTAGACTTGCCCGCAATTTG‑TTTTTTCGTGAAGAGGCCAAGCCCGTTTCGGCTGTCCATTGAGTTCCGCCGGTTGTCACCCa                                                                                                    <  1:270110/140‑1 (MQ=255)

                                        ggAAACTGCTTGATCCTAATTTGTTTTGCGCATGTCGTTAAACGGGTAGAAAACAAACTTAGACTTGCCCGCAATTTG‑TTTTTTCGTGAAGAGGCCAAGCCCGTTTCGGCTGTCCATTGAGTTCCGCCGGTTGTCACCCa                                                                                                    <  1:703555/140‑1 (MQ=255)

                                         gAAACTGCTTGATCCTAATTTGTTTTGCGCATGTCGTTAAACGGGTAGAAAACAAACTTAGACTTGCCCGCAATTTG‑TTTTTTCGTGAAGAGGCCAAGCCCGTTTCGGCTGTCCATTGAGTTCCGCCGGTTGTCACCCAt                                                                                                   >  2:799876/1‑140 (MQ=255)

                                            aCTGCTTGATCCTAATTTGTTTTGCGCATGTCGTTAAACGGGTAGAAAACAAACTTAGACTTGCCCGCAATTTG‑TTTTTTCGTGAAGAGGCCAAGCCCGTTTCGGCTGTCCATTGAGTTCCGCCGGTTGTCACCCATcac                                                                                                >  2:627344/1‑140 (MQ=255)

                                            aCTGCTTGATCCTAATTTGTTTTGCGCATGTCGTTAAACGGGTAGAAAACAAACTTAGACTTGCCCGCAATTTG‑TTTTTTCGTGAAGAGGCCAAGCCCGTTTCGGCTGTCCATTGAGTTCCGCCGGTTGTCACCCATcac                                                                                                >  2:2358732/1‑140 (MQ=255)

                                             cTGCTTGATCCTAATTTGTTTTGCGCATGTCGTTAAACGGGTAGAAAACAAACTTAGACTTGCCCGCAATTTG‑TTTTTTCGTGAAGAGGCCAAGCCCGTTTCGGCTGTCCATTGAGTTCCGCCGGTTGTCACCCATcaca                                                                                               <  1:2358732/140‑1 (MQ=255)

                                             cTGCTTGATCCTAATTTGTTTTGCGCATGTCGTTAAACGGGTAGAAAACAAACTTAGACTTGCCCGCAATTTG‑TTTTTTCGTGAAGAGGCCAAGCCCGTTTCGGCTGTCCATTGAGTTCCGCCGGTTGTCACCCATcaca                                                                                               <  1:627344/140‑1 (MQ=255)

                                                cTTGATCCTAATTTGTTTTGCGCATTTCGTTAAACGGGTAGAAAACAAACTTTGACGTACCCGCAATTTG‑TTTTTTCGTGAAGAGGCCAAGGCCGTTACGGCTGTCCa                                                                                                                            >  2:1729474/1‑108 (MQ=255)

                                                cTTGATCCTAATTTGTTTTGCGCATTTCGTTAAACGGGTAGAAAACAAACTTTGACGTACCCGCAATTTG‑TTTTTTCGTGAAGAGGCCAAGGCCGTTACGGCTGTCCa                                                                                                                            <  1:1729474/108‑1 (MQ=255)

                                                cTTGATCCTAATTTGTTTTGCGCATTTCGTTAAACGGGTAGAAAACAAACTTTGACGTACCCGCAATTTG‑TTTTTTCGTGAAGAGGCCAAGGCCGTTACGGCTGTCCa                                                                                                                            >  2:2095084/1‑108 (MQ=255)

                                                cTTGATCCTAATTTGTTTTGCGCATTTCGTTAAACGGGTAGAAAACAAACTTTGACGTACCCGCAATTTG‑TTTTTTCGTGAAGAGGCCAAGGCCGTTACGGCTGTCCa                                                                                                                            <  1:2095084/108‑1 (MQ=255)

                                                cTTGATCCTAATTTGTTTTGCGCATGTCGTTAAACGGGTAGAAAACAAACTTAGACTTGCCCGCAATTTG‑TTTTTTCGTGAAGAGGCCAAGCCCGTTTCGGCTGTCCATTGAGTTCCGCCGGTTGTCACCCATCACaaaa                                                                                            >  2:1434183/1‑140 (MQ=255)

                                                cTTGATCCTAATTTGTTTTGCGCATGTCGTTAAACGGGTAGAAAACAAACTTAGACTTGCCCGCAATTTG‑TTTTTTCGTGAAGAGGCCAAGCCCGTTTCGGCTGTCCATTGAGTTCCGCCGGTTGTCACCCATCACaaaa                                                                                            >  2:474955/1‑140 (MQ=255)

                                                              gTTTTGCGCATGTCGTTAAACGGGTAGAAAACAAACTTAGACTTGCCCGCAATTTG‑TTTTTTCGTGAAGAGGCCAAGCCCGTTTCGGCTGTCCATTGAGTTCCGCCGGTTGTCACCCATCACAAAATACTTGTCATCAgg                                                                              <  1:1245796/140‑1 (MQ=255)

                                                              gTTTTGCGCATGTCGTTAAACGGGTAGAAAACAAACTTAGACTTGCCCGCAATTTG‑TTTTTTCGTGAAGAGGCCAAGCCCGTTTCGGCTGTCCATTGAGTTCCGCCGGTTGTCACCCATCACAAAATACTTGTCATCAgg                                                                              <  1:2384809/140‑1 (MQ=255)

                                                              gTTTTGCGCATGTCGTTAAACGGGTAGAAAACAAACTTAGACTTGCCCGCAATTTG‑TTTTTTCGTGAAGAGGCCAAGCCCGTTTCGGCTGTCCATTGAGTTCCGCCGGTTGTCACCCATCACAAAATACTTGTCATCAgg                                                                              <  1:1983997/140‑1 (MQ=255)

                                                              gTTTTGCGAATGTCGTTAAACGGGTAGAAAACAAACTTAGACTTGCCCGCAATTTG‑TTTTTTCGTGAAGAGGCCAAGCCCGTTTCGGCTGTCCATTGAGTTCCGCCGGTTGTCACCCATCACAAAATACTTGTCATCAgg                                                                              <  2:1875371/140‑1 (MQ=255)

                                                                    cgcATGTCGTTAAACGGGTAGAAAACAAACTTAGACTTGCCCGCAATTTG‑TTTTTTCGTGAAGAGGCCAAGCCCGTTTCGGCTGTCCATTGAGTTCCGCCGGTTGTCACCCATCACAAAATACTTGTCATCAGGCACttt                                                                        >  2:1064952/1‑140 (MQ=255)

                                                                                   gggTAGAAAACAAACTTAGACTTGCCCGCAATTTGTTTTTTTCGGGAATTGGCCAAGCCCGTTTCGGCTTTCCATTGAGTGCCGCCGGTTGTCACCCAGCACAAAAAACTTTTCATCAGGCACTTTTATCGGGCCGAAat                                                          >  1:1796652/1‑140 (MQ=255)

                                                                                   gggTAGAAAACAAACTTAGACTTGCCCGCAATTTG‑TTTTTTCGTGAAGAGGCCAAGCCCGTTTCGGCTGTCCATTGAGTTCCGCCGGTTGTCACCCATCACAAAATACTTGTCATCAGGCACTTTGATCGGGCCGAAatc                                                         >  1:508952/1‑140 (MQ=255)

                                                                                   gggTAGAAAACAAACTTAGACTTGCCCGCAATTTG‑TTTTTTCGTGAAGAGGCCAAGCCCGTTTCGGCTGTCCAGTGAGTTCCGCCGGTTGTCACCCATCACAAAATACTTGTCATCAGGCACTTTGATCGGGCCGAAatc                                                         >  1:2364778/1‑140 (MQ=255)

                                                                                     gTAGAAAACAAACTTAGACTTGCCCGCAATTTG‑TTTTTTCGTGAAGAGGCCAAGCCCGTTTCGGCTGTCCATTGAGTTCCGCCGGTTGTCACCCATCACAAAATACTTGTCATCAGGCACTTTGATCGGGCCGAAatcat                                                       <  1:1453433/140‑1 (MQ=255)

                                                                                     gTAGAAAACAAACTTAGACTTGCCCGCAATTTG‑TTTTTTCGTGAAGAGGCCAAGCCCGTTTCGGCTGTCCATTGAGTTCCGCCGGTTGTCACCCATCACAAAATACTTGTCATCAGGCACTTTGATCGGGCCGAAatcat                                                       <  1:1254824/140‑1 (MQ=255)

                                                                                       aGAAAACAAACTTAGACTTGCCCGCAATTTG‑TTTTTTCGTGAAGAGGCCAAGCCCGTTTCGGCTGTCCATTGAGTTCCGCCGGTTGTCACCCATCACAAAATACTTGTCATCAGGCACTTTGATCGGGCCGAAATCATCg                                                     >  1:2336838/1‑140 (MQ=255)

                                                                                             caaacTTAGACTTGCCCGCAATTTG‑TTTTTTCGTGAAGAGGCCAAGCCCGTTTCGGCTGTCCATTGAGTTCCGCCGGTTGTCACCCATCACAAAATACTTGTCATCAGGCACTTTGATCGGGCCGAAATCATCGGTTaaa                                               <  1:474955/140‑1 (MQ=255)

                                                                                             caaacTTAGACTTGCCCGCAATTTG‑TTTTTTCGTGAAGAGGCCAAGCCCGTTTCGGCTGTCCATTGAGTTCCGCCGGTTGTCACCCATCACAAAATACTTGTCATCAGGCACTTTGATCGGGCCGAAATCATCGGTTaaa                                               <  1:1434183/140‑1 (MQ=255)

                                                                                                acTTAGACTTGCCCGCAATTTG‑TTTTTTCGTGAAGAGGCCAAGCCCGTTTCGGCTGTCCATTGAGTTCCGCCGGTTGTCACCCATCACAAAATACTTGTCATCAGGCACTTTGATCGGGCCGAAATCATCGGTTAAATTg                                            >  1:1621682/1‑140 (MQ=255)

                                                                                                acTTAGACTTGCCCGCAATTTG‑TTTTTTCGTGAAGAGGCCAAGCCCGTTTCGGCTGTCCATTGAGTTCCGCCGGTTGTCACCCATCACAAAATACTTGTCATCAGGCACTTTGATCGGGCCGAAATCATCGGTTAAATTg                                            >  1:1917328/1‑140 (MQ=255)

                                                                                                acTTAGACTTGCCCGCAATTTG‑TTTTTTCGTGAAGAGGCCAAGCCCGTTTCGGCTGTCCATTGAGTTCCGCCGGTTGTCACCCATCACAAAATACTTGTCATCAGGCACTTTGATCGGGCCGAAATCATCGGTTAAATTg                                            >  1:2183969/1‑140 (MQ=255)

                                                                                                          gCCCGCAATTTG‑TTTTTTCGTGAAGAGGCCAAGCCCGTTTCGGCTGTCCATTGAGTTCCGCCGGTTGTCACCCATCACAAAATACTTGTCATCAGGCACTTTGATCGGGCCGAAATCATCGGTTAAATTGTCAAAACCtt                                  >  2:2317135/1‑140 (MQ=255)

                                                                                                          gCCCGCAATTTG‑TTTTTTCGTGAAGAGGCCAAGCCCGTTTCGGCTGTCCATTGAGTTCCGCCGGTTGTCACCCATCACAAAATACTTGTCATCAGGCACTTTGATCGGGCCGAAATCATCGGTTAAATTGTCAAAACCtt                                  >  2:2267833/1‑140 (MQ=255)

                                                                                                          gCCCGCAATTTG‑TTTTTTCGTGAAGAGGCCAAGCCCGTTTCGGCTGTCCATTGAGTTCCGCCGGTTGTCACCCATCACAAAATACTTGTCATCAGGCACTTTGATCGGGCCGAAATCATCGGTTAAATTGTCAAAACCtt                                  >  2:903384/1‑140 (MQ=255)

                                                                                                          gCCCGCAATTTG‑TTTTTTCGTGAAGAGGCCAAGCCCGTTTCGGCTGTCCATTGAGTTCCGCCGGTTGTCACCCATCACAAAATACTTGTCATCAGGCACTTTGATCGGGCCGAAATCATCGGTTAAATTGTCAAAACCtt                                  >  2:2402544/1‑140 (MQ=255)

                                                                                                          gCCCGCAATTTG‑TTTTTTCGTGAAGAGGCCAAGCCCGTTTCGGCTGTCCATTGAGTTCCGCCGGTTGTCACCCATCACAAAATACTTGTCATCAGGCACTTTGATCGGGCCGAAATCATCGGTTAAATTGTCAAAACCtt                                  >  1:1069503/1‑140 (MQ=255)

                                                                                                          gCCCGCAATTTG‑TTTTTTCGTGAAGAGGCCAAGCCCGTTTCGGCTGTCCATTGAGTTCCGCCGGTTGTCACCCATCACAAAATACTTGTCATCAGGCACTTTGATCGGGCCGAAATCATCGGTTAAATTGTCAAAACCtt                                  >  2:2051608/1‑140 (MQ=255)

                                                                                                          gCCCGCAATTTG‑TTTTTTCGTGAAGAGGCCAAGCCCGTTTCGGCTGTCCATTGAGTTCCGCCGGTTGTCACCCATCACAAAATACTTGTCATCAGGCACTTTGATCGGGCCGAAATCATCGGTTAAATTGTCAAAACCtt                                  >  1:1119875/1‑140 (MQ=255)

                                                                                                          gCCCGCAATTTG‑TTTTTTCGTGAAGAGGCCAAGCCCGTTTCGGCTGTCCATTGAGTTCCGCCGGTTGTCACCCATCACAAAATACTTGTCATCAGGCACTTTGATCGGGCCGAAATCATCGGTTAAATTGTCAAAACCtt                                  >  1:1340576/1‑140 (MQ=255)

                                                                                                          gCCCGCAATTTG‑TTTTTTCGTGAAGAGGCCAAGCCCGTTTCGGCTGTCCATTGAGTTCCGCCGGTTGTCACCCATCACAAAATACTTGTCATCAGGCACTTTGATCGGGCCGAAATCATCGGTTAAATTGTCAAAACCtt                                  >  1:2200973/1‑140 (MQ=255)

                                                                                                          gCCCGCAATTTG‑TTTTTTCGTGAAGAGGCCAAGCCCGTTTCGGCTGTCCATTGAGTTCCGCCGGTTGTCACCCATCACAAAATACTTGTCATCAGGCACTTTGATCGGGCCGAAATCATCGGTTAAATTGTCAAAACCtt                                  >  1:2233744/1‑140 (MQ=255)

                                                                                                          gCCCGCAATTTG‑TTTTTTCGTGAAGAGGCCAAGCCCGTTTCGGCTGTCCATTGAGTTCCGCCGGTTGTCACCCATCACAAAATACTTGTCATCAGGCACTTTGATCGGGCCGAAATCATCGGTTAAATTGTCAAAACCtt                                  >  1:2252604/1‑140 (MQ=255)

                                                                                                          gCCCGCAATTTG‑TTTTTTCGTGAAGAGGCCAAGCCCGTTTCGGCTGTCCATTGAGTTCCGCCGGTTGTCACCCATCACAAAATACTTGTCATCAGGCACTTTGATCGGGCCGAAATCATCGGTTAAATTGTCAAAACCtt                                  >  1:2292682/1‑140 (MQ=255)

                                                                                                          gCCCGCAATTTG‑TTTTTTCGTGAAGAGGCCAAGCCCGTTTCGGCTGTCCATTGAGTTCCGCCGGTTGTCACCCATCACAAAATACTTGTCATCAGGCACTTTGATCGGGCCGAAATCATCGGTTAAATTGTCAAAACCtt                                  >  1:938823/1‑140 (MQ=255)

                                                                                                          gCCCGCAATTTG‑TTTTTTCGTGAAGAGGCCAAGCCCGTTTCGGCTGTCCATTGAGTTCCGCCGGTTGTCACCCATCACAAAATACTTGTCATCAGGCACTTTGATCGGGCCGAAATCATCGGTTAAATTGTCAAAACCtt                                  >  2:2051647/1‑140 (MQ=255)

                                                                                                           cccGCGATTTG‑TTTTTTCGTGAAGAGGCCAAGCCCGTTTCGGCTGTCCATTGAGTTCCGCCGGTTGTCACCCATCACAAAATACTTGTCATCAGGCACTTTGATCGGGCCGAAATCATCGGTTAAATTGTCAAAACCTTc                                 >  1:1927614/1‑140 (MQ=255)

                                                                                                           cccGCAATTTG‑TTTTTTCGTGAAGAGGCCAAGCCCGTTTCGGCTGTCCATTGAGTTCCGCCGGTTGTCACCCATCACAAAATACTTGTCATCAGGCACTTTGATCGGGCCGAAATCATCGGTTAAATTGTCAAAACCTTc                                 >  1:522265/1‑140 (MQ=255)

                                                                                                           cccGCAATTTG‑TTTTTTCGTGAAGAGGCCAAGCCCGTTTCGGCTGTCCATTGAGTTCCGCCGGTTGTCACCCATCACAAAATACTTGTCATCAGGCACTTTGATCGGGCCGAAATCATCGGTTAAATTGTCAAAACCTTc                                 >  1:1805666/1‑140 (MQ=255)

                                                                                                           cccGCAATTTG‑TTTTTTCGTGAAGAGGCCAAGCCCGTTTCGGCTGTCCATTGAGTTCCGCCGGTTGTCACCCATCACAAAATACTTGTCATCAGGCACTTTGATCGGGCCGAAATCATCGGTTAAATTGTCAAAACCTTc                                 >  1:1805374/1‑140 (MQ=255)

                                                                                                                        tttttCGTGAAGAGGCCAAGCCCGTTTCGGCTGTCCATTGAGTTCCGCCGGTTGTCACCCATCACAAAATACTTGTCATCAGGCACTTTGATCGGGCCGAAATCATCGGTTAAATTGTCAAAACCTTCATTTTTGGcttt                     <  1:2144334/140‑1 (MQ=255)

                                                                                                                         ttttCGTGAAGAGGCCAAGCCCGTTTCGGCTGTCCATTGAGTTCCGCCGGTTGTCACCCATCACAAAATACTTGTCATCAGGCACTTTGATCGGGCCGAAATCATCGGTTAAATTGTCAAAACCTTCATTTTTGGctttc                    <  2:938823/140‑1 (MQ=255)

                                                                                                                                 aaGAGGCCAAGCCCGTTTCGGCTGTCCATTGAGTTCCGCCGGTTGTCACCCATCACAAAATACTTGTCATCAGGCACTTTGATCGGGCCGAAATCATCGGTTAAATTGTCAAAACCTTCATTTTTGGCTTTCTTTTtata            <  1:2070572/140‑1 (MQ=255)

                                                                                                                                 aaGAGGCCAAGCCCGTTTCGGCTGTCCATTGAGTTCCGCCGGTGGTCACCCATCACAAAATACTTGTCAACAGGCACTTTGATCGGGCCGAAAACATCGGTTAAATTGTCAAAACCTTCATTTTTGGCTTTCTTTTtata            <  1:2404078/140‑1 (MQ=255)

                                                                                                                                      gCCAAGCCCGTTTCGGCTGTCCATTGAGTTCCGCCGGTTGTCACCCATCACAAAATACTTGTCATCAGGCACTTTGATCGGGCCGAAATCATCGGTTAAATTGTCAAAACCTTCATTTTTGGCTTTCTTTTTATATGCCt       <  2:405358/140‑1 (MQ=255)

                                                                                                                                      gCCAAGCCCGTTTCGGCTGTCCATTGAGTTCCGCCGGTTGTCACCCATCACAAAATACTTGTCATCAGGCACTTTGATCGGGCCGAAATCATCGGTTAAATTGTCAAAACCTTCATTTTTGGCTTTCTTTTTATATGCCt       <  2:1221576/140‑1 (MQ=255)

                                                                                                                                           gCCCGTTTCGGCTGTCCATTGAGTTCCGCCGGTTGTCACCCATCACAAAATACTTGTCATCAGGCACTTTGATCGGGCCGAAATCATCGGTTAAATTGTCAAAACCTTCATTTTTGGCTTTCTTTTTATATGCCTTCaaa  <  2:1731952/140‑1 (MQ=255)

ATGAACGAAAAAAGAAATAAAAAAAGCAGCCTCCTGTTCAGGAAACTGCTTGATCCTAATTTGTTTTGCGCATGTCGTTAAACGGGTAGAAAACAAACTTAGACTTGCCCGCAATTTG‑TTTTTTCGTGAAGAGGCCAAGCCCGTTTCGGCTGTCCATTGAGTTCCGCCGGTTGTCACCCATCACAAAATACTTGTCATCAGGCACTTTGATCGGGCCGAAATCATCGGTTAAATTGTCAAAACCTTCATTTTTGGCTTTCTTTTTATATGCCTTCAAA  >  NZ_CP034943/2301591‑2301868

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`