BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	2,301,736	T→A	5.5%	R155R (CGA→CGT)	lepB ←	signal peptidase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	2,301,736	0	T	A	5.5%	247.5 / 4.6	73	R155R (CGA→CGT)	lepB	signal peptidase I
Reads supporting (aligned to +/- strand): ref base T (33/36); new base A (2/2); total (35/38)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01

GAAAAAAGAAATAAAAAAAGCAGCCTCCTGTTCAGGAAACTGCTTGATCCTAATTTGTTTTGCGCATGTCGTTAAACGGGTAGAAAACAAACTTAGACTTGCCCGCAATTTG‑TTTTTTCGTGAAGAGGCCAAGCCCGTTTCGGCTGTCCATTGAGTTCCGCCGGTTGTCACCCATCACAAAATACTTGTCATCAGGCACTTTGATCGGGCCGAAATCATCGGTTAAATTGTCAAAACCTTCATTTTTGGCTTTCTTTTTATATGCCTTCAAATAAGG  >  NZ_CP034943/2301597‑2301873

gAAAAAAGAAATAAAAAAAGCAGCCTCCTGTTCAGGAAACTGCTTGATCCTAATTTGTTTTGCGCATGTCGTTAAACGGGTAGAAAACAAACTTAGACTTGCCCGCAATTTG‑TTTTTTCGTGAAGAGGCCAAGCCCGttt                                                                                                                                           >  1:1221576/1‑140 (MQ=255)

gAAAAAAGAAATAAAAAAAGCAGCCTCCTGTTCAGGAAACTGCTTGATCCTAATTTGTTTTGCGCATGTCGTTAAACGGGTAGAAAACAAACTTAGACTTGCCCGCAATTTG‑TTTTTTCGTGAAGAGGCCAAGCCCGttt                                                                                                                                           >  1:405358/1‑140 (MQ=255)

  aaaaaGAAATAAAAAAAGCAGCCTCCTGTTCAGGAAACTGCTTGATCCTAATTTGTTTTGCGCATGTCGTTAAACGGGTAGAAAACAAACTTAGACTTGCCCGCAATTTG‑TTTTTTCGTGAAGAGGCCAAGCCCGTTTCg                                                                                                                                         >  2:740004/1‑140 (MQ=255)

     aaGAAATAAAAAAAGCAGCCTCCTGTTCAGGAAACTGCTTGATCCTAATTTGTTTTGCGCATGTCGTTAAACGGGTAGAAAACAAACTTAGACTTGCCCGCAATTTG‑TTTTTTCGTGAAGAGGCCAAGCCCGTTTCGGCt                                                                                                                                      <  1:1947360/140‑1 (MQ=255)

     aaGAAATAAAAAAAGCAGCCTCCTGTTCAGGAAACTGCTTGATCCTAATTTGTTTTGCGCATGTCGTTAAACGGGTAGAAAACAAACTTAGACTTGCCCGCAATTTG‑TTTTTTCGTGAAGAGGCCAAGCCCGTTTCGGCt                                                                                                                                      <  1:621899/140‑1 (MQ=255)

     aaGAAATAAAAAAAGCAGCCTCCTGTTCAGGAAACTGCTTGATCCTAATTTGTTTTGCGCATGTCGTTAAACGGGTAGAAAACAAACTTAGACTTGCCCGCAATTTG‑TTTTTTCGTGAAGAGGCCAAGCCCGTTTCGGCt                                                                                                                                      <  1:621145/140‑1 (MQ=255)

     aaGAAATAAAAAAAGCAGCCTCCTGTTCAGGAAACTGCTTGATCCTAATTTGTTTTGCGCATGTCGTTAAACGGGTAGAAAACAAACTTAGACTTGCCCGCAATTTG‑TTTTTTCGTGAAGAGGCCAAGCCCGTTTCGGCt                                                                                                                                      <  2:1291922/140‑1 (MQ=255)

     aaGAAATAAAAAAAGCAGCCTCCTGTTCAGGAAACTGCTTGATCCTAATTTGTTTTGCGCATGTCGTTAAACGGGTAGAAAACAAACTTAGACTTGCCCGCAATTTG‑TTTTTTCGTGAAGAGGCCAAGCCCGTTTCGGCt                                                                                                                                      <  2:2283282/140‑1 (MQ=255)

     aaGAAATAAAAAAAGCAGCCTCCTGTTCAGGAAACTGCTTGATCCTAATTTGTTTTGCGCATGTCGTTAAACGGGTAGAAAACAAACTTAGACTTGCCCGCAATTTG‑TTTTTTCGTGAAGAGGCCAAGCCCGTTTCGGCt                                                                                                                                      <  2:407459/140‑1 (MQ=255)

     aaGAAATAAAAAAAGCAGCCTCCTGTTCAGGAAACTGCTTGATCCTAATTTGTTTTGCGCATGTCGTTAAACGGGTAGAAAACAAACTTAGACTTGCCCGCAATTTG‑TTTTTTCGTGAAGAGGCCAAGCCCGTTTCGGCt                                                                                                                                      <  2:894981/140‑1 (MQ=255)

     aaGAAATAAAAAAAGCAGCCTCCTGTTCAGGAAACTGCTTGATCCTAATTTGTTTTGCGCATGTCGTTAAACGGGTAGAAAACAAACTTAGACTTGCCCGCAATTTG‑TTTTTTCGTGAAGAGGCCAAGCCCGTTTCGGCt                                                                                                                                      <  2:825584/140‑1 (MQ=255)

                aaaGCAGCCTCCTGTTCAGGAAACTGCTTGATCCTAATTTGTTTTGCGCATGTCTTTAAACGGGTAGAAAACAAACTTAGACTTGCCCGCAATTTG‑TTTTTTCGTGAAGATTCAATTACAGTTTCGGCTGTCCATTgttt                                                                                                                           <  2:1491014/140‑4 (MQ=255)

                  agcagcCTCCTGTTCAGGAAACTGCTTGATCCTAATTTGTTTTGCGCATGTCGTTAAACGGGTAGAAAACAAACTTAGACTTGCCCGCAATTTG‑TTTTTTCGTGAAGAGGCCAAGCCCGTTTCGGCTGTCCATTGAGTTc                                                                                                                         >  2:754933/1‑140 (MQ=255)

                     agcCTCCTGTTCAGGAAACTGCTTGATCCTACTTTGTTTTGCGCAGGTCGTTAAACGGGTAGAAAACAAACTTAGACTTGCCCGCAATTTG‑TTTTTTCGTGAAGAGGCCAAGCCCGTTTCGGCTGTCCATTGAGTTccgc                                                                                                                      <  1:1039518/140‑1 (MQ=255)

                     agcCTCCTGTTCAGGAAACTGCTTGATCCTAATTTGTTTTGCGCATGTCGTTAAACGGGTAGAAAACAAACTTAGACTTGCCCGCAATTTG‑TTTTTTCGTGAAGAGGCCAAGCCCGTTTCGGCTGTCCATTGAGTTccgc                                                                                                                      <  2:259021/140‑1 (MQ=255)

                     agcCTCCTGTTCAGGAAACTGCTTGATCCTAATTTGTTTTGCGCATGTCGTTAAACGGGTAGAAAACAAACTTAGACTTGCCCGCAATTTG‑TTTTTTCGTGAAGAGGCCAAGCCCGTTTCGGCTGTCCATTGAGTTccgc                                                                                                                      <  1:2194278/140‑1 (MQ=255)

                     agcCTCCTGTTCAGGAAACTGCTTGATCCTAATTTGTTTTGCGCATGTCGTTAAACGGGTAGAAAACAAACTTAGACTTGCCCGCAATTTG‑TTTTTTCGTGAAGAGGCCAAGCCCGTTTCGGCTGTCCATTGAGTTccgc                                                                                                                      <  1:800325/140‑1 (MQ=255)

                     agcCTCCTGTTCAGGAAACTGCTTGATCCTAATTTGTTTTGCGCATGTCGTTAAACGGGTAGAAAACAAACTTAGACTTGCCCGCAATTTG‑TTTTTTCGTGAAGAGGCCAAGCCCGTTTCGGCTGTCCATTGAGTTccgc                                                                                                                      <  2:220174/140‑1 (MQ=255)

                     agcCTCCTGTTCAGGAAACTGCTTGATCCTAATTTGTTTTGCGCATGTCGTTAAACGGGGAGAAAACAAACTTAGACTTGCCCGCAATTTG‑TTTTTTCGTGAAGAGGCCAAGCCCGTTTCGGCTGTCCATTGAGTTccgc                                                                                                                      <  1:14442/140‑1 (MQ=255)

                              ttCAGGAAACTGCTTGATCCTAATTTGTTTTGCGCATGTCGTTAAACGGGTAGAAAACAAACTTAGACTTGCCCGCAATTTG‑TTTTTTCGTGAAGAGGCCAAGCCCGTTTCGGCTGTCCATTGAGTTCCGCCGGTTGTCa                                                                                                             <  2:1075323/140‑1 (MQ=255)

                                  ggAAACTGCTTGATCCTAATTTGTTTTGCGCATGTCGTTAAACGGGTAGAAAACAAACTTAGACTTGCCCGCAATTTG‑TTTTTTCGTGAAGAGGCCAAGCCCGTTTCGGCTGTCCATTGAGTTCCGCCGGTTGTCACCCa                                                                                                         <  1:703555/140‑1 (MQ=255)

                                  ggAAACTGCTTGATCCTAATTTGTTTTGCGCATGTCGTTAAACGGGTAGAAAACAAACTTAGACTTGCCCGCAATTTG‑TTTTTTCGTGAAGAGGCCAAGCCCGTTTCGGCTGTCCATTGAGTTCCGCCGGTTGTCACCCa                                                                                                         <  1:701589/140‑1 (MQ=255)

                                  ggAAACTGCTTGATCCTAATTTGTTTTGCGCATGTCGTTAAACGGGTAGAAAACAAACTTAGACTTGCCCGCAATTTG‑TTTTTTCGTGAAGAGGCCAAGCCCGTTTCGGCTGTCCATTGAGTTCCGCCGGTTGTCACCCa                                                                                                         <  1:270110/140‑1 (MQ=255)

                                   gAAACTGCTTGATCCTAATTTGTTTTGCGCATGTCGTTAAACGGGTAGAAAACAAACTTAGACTTGCCCGCAATTTG‑TTTTTTCGTGAAGAGGCCAAGCCCGTTTCGGCTGTCCATTGAGTTCCGCCGGTTGTCACCCAt                                                                                                        >  2:799876/1‑140 (MQ=255)

                                      aCTGCTTGATCCTAATTTGTTTTGCGCATGTCGTTAAACGGGTAGAAAACAAACTTAGACTTGCCCGCAATTTG‑TTTTTTCGTGAAGAGGCCAAGCCCGTTTCGGCTGTCCATTGAGTTCCGCCGGTTGTCACCCATcac                                                                                                     >  2:2358732/1‑140 (MQ=255)

                                      aCTGCTTGATCCTAATTTGTTTTGCGCATGTCGTTAAACGGGTAGAAAACAAACTTAGACTTGCCCGCAATTTG‑TTTTTTCGTGAAGAGGCCAAGCCCGTTTCGGCTGTCCATTGAGTTCCGCCGGTTGTCACCCATcac                                                                                                     >  2:627344/1‑140 (MQ=255)

                                       cTGCTTGATCCTAATTTGTTTTGCGCATGTCGTTAAACGGGTAGAAAACAAACTTAGACTTGCCCGCAATTTG‑TTTTTTCGTGAAGAGGCCAAGCCCGTTTCGGCTGTCCATTGAGTTCCGCCGGTTGTCACCCATcaca                                                                                                    <  1:627344/140‑1 (MQ=255)

                                       cTGCTTGATCCTAATTTGTTTTGCGCATGTCGTTAAACGGGTAGAAAACAAACTTAGACTTGCCCGCAATTTG‑TTTTTTCGTGAAGAGGCCAAGCCCGTTTCGGCTGTCCATTGAGTTCCGCCGGTTGTCACCCATcaca                                                                                                    <  1:2358732/140‑1 (MQ=255)

                                          cTTGATCCTAATTTGTTTTGCGCATTTCGTTAAACGGGTAGAAAACAAACTTTGACGTACCCGCAATTTG‑TTTTTTCGTGAAGAGGCCAAGGCCGTTACGGCTGTCCa                                                                                                                                 <  1:2095084/108‑1 (MQ=255)

                                          cTTGATCCTAATTTGTTTTGCGCATTTCGTTAAACGGGTAGAAAACAAACTTTGACGTACCCGCAATTTG‑TTTTTTCGTGAAGAGGCCAAGGCCGTTACGGCTGTCCa                                                                                                                                 <  1:1729474/108‑1 (MQ=255)

                                          cTTGATCCTAATTTGTTTTGCGCATTTCGTTAAACGGGTAGAAAACAAACTTTGACGTACCCGCAATTTG‑TTTTTTCGTGAAGAGGCCAAGGCCGTTACGGCTGTCCa                                                                                                                                 >  2:2095084/1‑108 (MQ=255)

                                          cTTGATCCTAATTTGTTTTGCGCATTTCGTTAAACGGGTAGAAAACAAACTTTGACGTACCCGCAATTTG‑TTTTTTCGTGAAGAGGCCAAGGCCGTTACGGCTGTCCa                                                                                                                                 >  2:1729474/1‑108 (MQ=255)

                                          cTTGATCCTAATTTGTTTTGCGCATGTCGTTAAACGGGTAGAAAACAAACTTAGACTTGCCCGCAATTTG‑TTTTTTCGTGAAGAGGCCAAGCCCGTTTCGGCTGTCCATTGAGTTCCGCCGGTTGTCACCCATCACaaaa                                                                                                 >  2:474955/1‑140 (MQ=255)

                                          cTTGATCCTAATTTGTTTTGCGCATGTCGTTAAACGGGTAGAAAACAAACTTAGACTTGCCCGCAATTTG‑TTTTTTCGTGAAGAGGCCAAGCCCGTTTCGGCTGTCCATTGAGTTCCGCCGGTTGTCACCCATCACaaaa                                                                                                 >  2:1434183/1‑140 (MQ=255)

                                                        gTTTTGCGCATGTCGTTAAACGGGTAGAAAACAAACTTAGACTTGCCCGCAATTTG‑TTTTTTCGTGAAGAGGCCAAGCCCGTTTCGGCTGTCCATTGAGTTCCGCCGGTTGTCACCCATCACAAAATACTTGTCATCAgg                                                                                   <  1:1245796/140‑1 (MQ=255)

                                                        gTTTTGCGCATGTCGTTAAACGGGTAGAAAACAAACTTAGACTTGCCCGCAATTTG‑TTTTTTCGTGAAGAGGCCAAGCCCGTTTCGGCTGTCCATTGAGTTCCGCCGGTTGTCACCCATCACAAAATACTTGTCATCAgg                                                                                   <  1:2384809/140‑1 (MQ=255)

                                                        gTTTTGCGCATGTCGTTAAACGGGTAGAAAACAAACTTAGACTTGCCCGCAATTTG‑TTTTTTCGTGAAGAGGCCAAGCCCGTTTCGGCTGTCCATTGAGTTCCGCCGGTTGTCACCCATCACAAAATACTTGTCATCAgg                                                                                   <  1:1983997/140‑1 (MQ=255)

                                                        gTTTTGCGAATGTCGTTAAACGGGTAGAAAACAAACTTAGACTTGCCCGCAATTTG‑TTTTTTCGTGAAGAGGCCAAGCCCGTTTCGGCTGTCCATTGAGTTCCGCCGGTTGTCACCCATCACAAAATACTTGTCATCAgg                                                                                   <  2:1875371/140‑1 (MQ=255)

                                                              cgcATGTCGTTAAACGGGTAGAAAACAAACTTAGACTTGCCCGCAATTTG‑TTTTTTCGTGAAGAGGCCAAGCCCGTTTCGGCTGTCCATTGAGTTCCGCCGGTTGTCACCCATCACAAAATACTTGTCATCAGGCACttt                                                                             >  2:1064952/1‑140 (MQ=255)

                                                                             gggTAGAAAACAAACTTAGACTTGCCCGCAATTTGTTTTTTTCGGGAATTGGCCAAGCCCGTTTCGGCTTTCCATTGAGTGCCGCCGGTTGTCACCCAGCACAAAAAACTTTTCATCAGGCACTTTTATCGGGCCGAAat                                                               >  1:1796652/1‑140 (MQ=255)

                                                                             gggTAGAAAACAAACTTAGACTTGCCCGCAATTTG‑TTTTTTCGTGAAGAGGCCAAGCCCGTTTCGGCTGTCCATTGAGTTCCGCCGGTTGTCACCCATCACAAAATACTTGTCATCAGGCACTTTGATCGGGCCGAAatc                                                              >  1:508952/1‑140 (MQ=255)

                                                                             gggTAGAAAACAAACTTAGACTTGCCCGCAATTTG‑TTTTTTCGTGAAGAGGCCAAGCCCGTTTCGGCTGTCCAGTGAGTTCCGCCGGTTGTCACCCATCACAAAATACTTGTCATCAGGCACTTTGATCGGGCCGAAatc                                                              >  1:2364778/1‑140 (MQ=255)

                                                                               gTAGAAAACAAACTTAGACTTGCCCGCAATTTG‑TTTTTTCGTGAAGAGGCCAAGCCCGTTTCGGCTGTCCATTGAGTTCCGCCGGTTGTCACCCATCACAAAATACTTGTCATCAGGCACTTTGATCGGGCCGAAatcat                                                            <  1:1254824/140‑1 (MQ=255)

                                                                               gTAGAAAACAAACTTAGACTTGCCCGCAATTTG‑TTTTTTCGTGAAGAGGCCAAGCCCGTTTCGGCTGTCCATTGAGTTCCGCCGGTTGTCACCCATCACAAAATACTTGTCATCAGGCACTTTGATCGGGCCGAAatcat                                                            <  1:1453433/140‑1 (MQ=255)

                                                                                 aGAAAACAAACTTAGACTTGCCCGCAATTTG‑TTTTTTCGTGAAGAGGCCAAGCCCGTTTCGGCTGTCCATTGAGTTCCGCCGGTTGTCACCCATCACAAAATACTTGTCATCAGGCACTTTGATCGGGCCGAAATCATCg                                                          >  1:2336838/1‑140 (MQ=255)

                                                                                       caaacTTAGACTTGCCCGCAATTTG‑TTTTTTCGTGAAGAGGCCAAGCCCGTTTCGGCTGTCCATTGAGTTCCGCCGGTTGTCACCCATCACAAAATACTTGTCATCAGGCACTTTGATCGGGCCGAAATCATCGGTTaaa                                                    <  1:474955/140‑1 (MQ=255)

                                                                                       caaacTTAGACTTGCCCGCAATTTG‑TTTTTTCGTGAAGAGGCCAAGCCCGTTTCGGCTGTCCATTGAGTTCCGCCGGTTGTCACCCATCACAAAATACTTGTCATCAGGCACTTTGATCGGGCCGAAATCATCGGTTaaa                                                    <  1:1434183/140‑1 (MQ=255)

                                                                                          acTTAGACTTGCCCGCAATTTG‑TTTTTTCGTGAAGAGGCCAAGCCCGTTTCGGCTGTCCATTGAGTTCCGCCGGTTGTCACCCATCACAAAATACTTGTCATCAGGCACTTTGATCGGGCCGAAATCATCGGTTAAATTg                                                 >  1:1621682/1‑140 (MQ=255)

                                                                                          acTTAGACTTGCCCGCAATTTG‑TTTTTTCGTGAAGAGGCCAAGCCCGTTTCGGCTGTCCATTGAGTTCCGCCGGTTGTCACCCATCACAAAATACTTGTCATCAGGCACTTTGATCGGGCCGAAATCATCGGTTAAATTg                                                 >  1:2183969/1‑140 (MQ=255)

                                                                                          acTTAGACTTGCCCGCAATTTG‑TTTTTTCGTGAAGAGGCCAAGCCCGTTTCGGCTGTCCATTGAGTTCCGCCGGTTGTCACCCATCACAAAATACTTGTCATCAGGCACTTTGATCGGGCCGAAATCATCGGTTAAATTg                                                 >  1:1917328/1‑140 (MQ=255)

                                                                                                    gCCCGCAATTTG‑TTTTTTCGTGAAGAGGCCAAGCCCGTTTCGGCTGTCCATTGAGTTCCGCCGGTTGTCACCCATCACAAAATACTTGTCATCAGGCACTTTGATCGGGCCGAAATCATCGGTTAAATTGTCAAAACCtt                                       >  2:2051647/1‑140 (MQ=255)

                                                                                                    gCCCGCAATTTG‑TTTTTTCGTGAAGAGGCCAAGCCCGTTTCGGCTGTCCATTGAGTTCCGCCGGTTGTCACCCATCACAAAATACTTGTCATCAGGCACTTTGATCGGGCCGAAATCATCGGTTAAATTGTCAAAACCtt                                       >  2:2051608/1‑140 (MQ=255)

                                                                                                    gCCCGCAATTTG‑TTTTTTCGTGAAGAGGCCAAGCCCGTTTCGGCTGTCCATTGAGTTCCGCCGGTTGTCACCCATCACAAAATACTTGTCATCAGGCACTTTGATCGGGCCGAAATCATCGGTTAAATTGTCAAAACCtt                                       >  2:2267833/1‑140 (MQ=255)

                                                                                                    gCCCGCAATTTG‑TTTTTTCGTGAAGAGGCCAAGCCCGTTTCGGCTGTCCATTGAGTTCCGCCGGTTGTCACCCATCACAAAATACTTGTCATCAGGCACTTTGATCGGGCCGAAATCATCGGTTAAATTGTCAAAACCtt                                       >  2:2317135/1‑140 (MQ=255)

                                                                                                    gCCCGCAATTTG‑TTTTTTCGTGAAGAGGCCAAGCCCGTTTCGGCTGTCCATTGAGTTCCGCCGGTTGTCACCCATCACAAAATACTTGTCATCAGGCACTTTGATCGGGCCGAAATCATCGGTTAAATTGTCAAAACCtt                                       >  1:938823/1‑140 (MQ=255)

                                                                                                    gCCCGCAATTTG‑TTTTTTCGTGAAGAGGCCAAGCCCGTTTCGGCTGTCCATTGAGTTCCGCCGGTTGTCACCCATCACAAAATACTTGTCATCAGGCACTTTGATCGGGCCGAAATCATCGGTTAAATTGTCAAAACCtt                                       >  2:2402544/1‑140 (MQ=255)

                                                                                                    gCCCGCAATTTG‑TTTTTTCGTGAAGAGGCCAAGCCCGTTTCGGCTGTCCATTGAGTTCCGCCGGTTGTCACCCATCACAAAATACTTGTCATCAGGCACTTTGATCGGGCCGAAATCATCGGTTAAATTGTCAAAACCtt                                       >  1:2292682/1‑140 (MQ=255)

                                                                                                    gCCCGCAATTTG‑TTTTTTCGTGAAGAGGCCAAGCCCGTTTCGGCTGTCCATTGAGTTCCGCCGGTTGTCACCCATCACAAAATACTTGTCATCAGGCACTTTGATCGGGCCGAAATCATCGGTTAAATTGTCAAAACCtt                                       >  1:2252604/1‑140 (MQ=255)

                                                                                                    gCCCGCAATTTG‑TTTTTTCGTGAAGAGGCCAAGCCCGTTTCGGCTGTCCATTGAGTTCCGCCGGTTGTCACCCATCACAAAATACTTGTCATCAGGCACTTTGATCGGGCCGAAATCATCGGTTAAATTGTCAAAACCtt                                       >  1:2233744/1‑140 (MQ=255)

                                                                                                    gCCCGCAATTTG‑TTTTTTCGTGAAGAGGCCAAGCCCGTTTCGGCTGTCCATTGAGTTCCGCCGGTTGTCACCCATCACAAAATACTTGTCATCAGGCACTTTGATCGGGCCGAAATCATCGGTTAAATTGTCAAAACCtt                                       >  1:2200973/1‑140 (MQ=255)

                                                                                                    gCCCGCAATTTG‑TTTTTTCGTGAAGAGGCCAAGCCCGTTTCGGCTGTCCATTGAGTTCCGCCGGTTGTCACCCATCACAAAATACTTGTCATCAGGCACTTTGATCGGGCCGAAATCATCGGTTAAATTGTCAAAACCtt                                       >  2:903384/1‑140 (MQ=255)

                                                                                                    gCCCGCAATTTG‑TTTTTTCGTGAAGAGGCCAAGCCCGTTTCGGCTGTCCATTGAGTTCCGCCGGTTGTCACCCATCACAAAATACTTGTCATCAGGCACTTTGATCGGGCCGAAATCATCGGTTAAATTGTCAAAACCtt                                       >  1:1340576/1‑140 (MQ=255)

                                                                                                    gCCCGCAATTTG‑TTTTTTCGTGAAGAGGCCAAGCCCGTTTCGGCTGTCCATTGAGTTCCGCCGGTTGTCACCCATCACAAAATACTTGTCATCAGGCACTTTGATCGGGCCGAAATCATCGGTTAAATTGTCAAAACCtt                                       >  1:1119875/1‑140 (MQ=255)

                                                                                                    gCCCGCAATTTG‑TTTTTTCGTGAAGAGGCCAAGCCCGTTTCGGCTGTCCATTGAGTTCCGCCGGTTGTCACCCATCACAAAATACTTGTCATCAGGCACTTTGATCGGGCCGAAATCATCGGTTAAATTGTCAAAACCtt                                       >  1:1069503/1‑140 (MQ=255)

                                                                                                     cccGCGATTTG‑TTTTTTCGTGAAGAGGCCAAGCCCGTTTCGGCTGTCCATTGAGTTCCGCCGGTTGTCACCCATCACAAAATACTTGTCATCAGGCACTTTGATCGGGCCGAAATCATCGGTTAAATTGTCAAAACCTTc                                      >  1:1927614/1‑140 (MQ=255)

                                                                                                     cccGCAATTTG‑TTTTTTCGTGAAGAGGCCAAGCCCGTTTCGGCTGTCCATTGAGTTCCGCCGGTTGTCACCCATCACAAAATACTTGTCATCAGGCACTTTGATCGGGCCGAAATCATCGGTTAAATTGTCAAAACCTTc                                      >  1:522265/1‑140 (MQ=255)

                                                                                                     cccGCAATTTG‑TTTTTTCGTGAAGAGGCCAAGCCCGTTTCGGCTGTCCATTGAGTTCCGCCGGTTGTCACCCATCACAAAATACTTGTCATCAGGCACTTTGATCGGGCCGAAATCATCGGTTAAATTGTCAAAACCTTc                                      >  1:1805374/1‑140 (MQ=255)

                                                                                                     cccGCAATTTG‑TTTTTTCGTGAAGAGGCCAAGCCCGTTTCGGCTGTCCATTGAGTTCCGCCGGTTGTCACCCATCACAAAATACTTGTCATCAGGCACTTTGATCGGGCCGAAATCATCGGTTAAATTGTCAAAACCTTc                                      >  1:1805666/1‑140 (MQ=255)

                                                                                                                  tttttCGTGAAGAGGCCAAGCCCGTTTCGGCTGTCCATTGAGTTCCGCCGGTTGTCACCCATCACAAAATACTTGTCATCAGGCACTTTGATCGGGCCGAAATCATCGGTTAAATTGTCAAAACCTTCATTTTTGGcttt                          <  1:2144334/140‑1 (MQ=255)

                                                                                                                   ttttCGTGAAGAGGCCAAGCCCGTTTCGGCTGTCCATTGAGTTCCGCCGGTTGTCACCCATCACAAAATACTTGTCATCAGGCACTTTGATCGGGCCGAAATCATCGGTTAAATTGTCAAAACCTTCATTTTTGGctttc                         <  2:938823/140‑1 (MQ=255)

                                                                                                                           aaGAGGCCAAGCCCGTTTCGGCTGTCCATTGAGTTCCGCCGGTTGTCACCCATCACAAAATACTTGTCATCAGGCACTTTGATCGGGCCGAAATCATCGGTTAAATTGTCAAAACCTTCATTTTTGGCTTTCTTTTtata                 <  1:2070572/140‑1 (MQ=255)

                                                                                                                           aaGAGGCCAAGCCCGTTTCGGCTGTCCATTGAGTTCCGCCGGTGGTCACCCATCACAAAATACTTGTCAACAGGCACTTTGATCGGGCCGAAAACATCGGTTAAATTGTCAAAACCTTCATTTTTGGCTTTCTTTTtata                 <  1:2404078/140‑1 (MQ=255)

                                                                                                                                gCCAAGCCCGTTTCGGCTGTCCATTGAGTTCCGCCGGTTGTCACCCATCACAAAATACTTGTCATCAGGCACTTTGATCGGGCCGAAATCATCGGTTAAATTGTCAAAACCTTCATTTTTGGCTTTCTTTTTATATGCCt            <  2:1221576/140‑1 (MQ=255)

                                                                                                                                gCCAAGCCCGTTTCGGCTGTCCATTGAGTTCCGCCGGTTGTCACCCATCACAAAATACTTGTCATCAGGCACTTTGATCGGGCCGAAATCATCGGTTAAATTGTCAAAACCTTCATTTTTGGCTTTCTTTTTATATGCCt            <  2:405358/140‑1 (MQ=255)

                                                                                                                                     gCCCGTTTCGGCTGTCCATTGAGTTCCGCCGGTTGTCACCCATCACAAAATACTTGTCATCAGGCACTTTGATCGGGCCGAAATCATCGGTTAAATTGTCAAAACCTTCATTTTTGGCTTTCTTTTTATATGCCTTCaaa       <  2:1731952/140‑1 (MQ=255)

                                                                                                                                          tttCGGCTGTCCATTGAGTTCCGCCGGTTGTCACCCa                                                                                                         >  2:498790/1‑37 (MQ=255)

                                                                                                                                          tttCGGCTGTCCATTGAGTTCCGCCGGTTGTCACCCa                                                                                                         <  1:498790/37‑1 (MQ=255)

                                                                                                                                          tttCGGCTGTCCATTGAGTTCCGCCGGTTGTCACCCCTCACAAAATACTTGTCATCAGGCACTTTGATCGGGCCGAAATCATCGGTTAAATTGTCAAAACCTTCATTTTTGGCTTTCTTTTTATATGCCTTCAAATAAgg  >  2:800851/1‑140 (MQ=255)

                                                                                                                                          tttCGGCTGTCCATTGAGTTCCGCCGGTTGTCACCCATCACAAAATACTTGTCATCAGGCACTTTGATCGGGCCGAAATCATCGGTTAAATTGTCAAAACCTTCATTTTTGGCTTTCTTTTTATATGCCTTCAAATAAgg  <  1:2222186/140‑1 (MQ=255)

                                                                                                                                          tttCGGCTGTCCATTGAGTTCCGCCGGTTGTCACCCATCACAAAATACTTGTCATCAGGCACTTTGATCGGGCCGAAATCATCGGTTAAATTGTCAAAACCTTCATTTTTGGCTTTCTTTTTATATGCCTTCAAATAAgg  >  2:657680/1‑140 (MQ=255)

                                                                                                                                          tttCGGCTGTCCATTGAGTTCCGCCGGTTGTCACCCATCACAAAATACTTGTCATCAGGCACTTTGATCGGGCCGAAATCATCGGTTAAATTGTCAAAACCTTCATTTTTGGCTTTCTTTTTATATGCCTTCAAATAAgg  >  1:1991146/1‑140 (MQ=255)

                                                                                                                                          tttCGGCTGTCCATTGAGTTCCGCCGGTTGTCACCCATCACAAAATACTTGTCATCAGGCACTTTGATCGGGCCGAAATCATCGGTTAAATTGTCAAAACCTTCATTTTTGGCTTTCTTTTTATATGCCTTCAAATAAgg  >  2:827820/1‑140 (MQ=255)

                                                                                                                                          tttCGGCTGTCCATTGAGTTCCGCCGGTTGTCACCCATCACAAAATACTTGTCATCAGGCACTTTGATCGGGCCGAAATCATCGGTTAAATTGTCAAAACCTTCATTTTTGGCTTTCTTTTTATATGCCTTCAAATAAgg  >  1:1544126/1‑140 (MQ=255)

                                                                                                                                          tttCGGCTGTCCATTGAGTTCCGCCGGTTGTCACCCATCACAAAATACTTGTCATCAGGCACTTTGATCGGGCCGAAATCATCGGTTAAATTGTCAAAACCTTCATTTTTGGCTTTCTTTTTATATGCCTTCAAATAAgg  <  1:148397/140‑1 (MQ=255)

                                                                                                                                          tttCGGCTGTCCATTGAGTTCCGCCGGTTGTCACCCATCACAAAATACTTGTCATCAGGCACTTTGATCGGGCCGAAATCATCGGTTAAATTGTCAAAACCTTCATTTTTGGCTTTCTTTTTATATGCATTCAAATAAgg  >  1:861924/1‑140 (MQ=255)

                                                                                                                                          tttCGGCTGTCCATTGAGTTCCGCCGGTTGTCACCCATCACAAAATACTTGTCATCAGGCACTTTGATCGGGCCGAAATCATCGGTTAAATTGTCAAAACCTTCAGTTTTGGCTTTCTTTTTATATGCCTTCAAATAAgg  >  1:1547494/1‑140 (MQ=255)

GAAAAAAGAAATAAAAAAAGCAGCCTCCTGTTCAGGAAACTGCTTGATCCTAATTTGTTTTGCGCATGTCGTTAAACGGGTAGAAAACAAACTTAGACTTGCCCGCAATTTG‑TTTTTTCGTGAAGAGGCCAAGCCCGTTTCGGCTGTCCATTGAGTTCCGCCGGTTGTCACCCATCACAAAATACTTGTCATCAGGCACTTTGATCGGGCCGAAATCATCGGTTAAATTGTCAAAACCTTCATTTTTGGCTTTCTTTTTATATGCCTTCAAATAAGG  >  NZ_CP034943/2301597‑2301873

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`