BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	240,198	G→T	12.0%	Q112K (CAA→AAA)	EO946_RS01360 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	240,198	0	G	T	12.0%	344.0 / 12.2	162	Q112K (CAA→AAA)	EO946_RS01360	hypothetical protein
Reads supporting (aligned to +/- strand): ref base G (57/80); new base T (23/2); total (80/82)
Fisher's exact test for biased strand distribution p-value = 1.69e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 164 total aligned reads displayed.

GTGGAGAAGGTGTAAGAGAGAGAGAAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAG‑TTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAG‑AGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCAAAATATAAAAATAGAGTTACTAAT  >  NZ_CP034943/240059‑240337

gtggAGAAGGTGTAAGAGAGAGAGAAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAG‑TTTTg                                                                                                                                              >  1:1699719/1‑140 (MQ=255)

   gagaAGGTGTAAGAGAGAGAGAAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAG‑TTTTGGga                                                                                                                                           <  1:2265990/140‑1 (MQ=255)

        ggTGTAAGAGAGAGAGAAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTTTTTTAAC‑TTTTTGGAGAttt                                                                                                                                      >  1:738576/1‑140 (MQ=255)

        ggTGTAAGAGAGAGAGAAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTTAAG‑TTTTGGGAGAttt                                                                                                                                      >  1:23590/1‑140 (MQ=255)

          tgtAAGAGAGAGAGAAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAG‑TTTTGGGAGATTTGa                                                                                                                                    >  2:9525/1‑140 (MQ=255)

           gtAAGAGAGAGAGAAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAG‑TTTTGGGAGATTTGaa                                                                                                                                   <  1:1046883/140‑1 (MQ=255)

           gtAAGAGAGAGAGAAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGATTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAG‑TTTTGGGAGATTTGaa                                                                                                                                   <  1:1047006/140‑1 (MQ=255)

                  agagagaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAG‑TTTTGGGATATTTGAATTGCttt                                                                                                                            >  2:770493/1‑140 (MQ=255)

                  agagagaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAG‑TTTTGGGAGATTTGAATGGCttt                                                                                                                            >  2:1301285/1‑140 (MQ=255)

                   gagagaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTTAAG‑TTTTTGGAGATTTGAATGGCtttt                                                                                                                           >  1:2272096/1‑140 (MQ=255)

                   gagagaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTAGGATTTTTTTTTTAAT‑TTTTTTGAGATTTTAAtttatttt                                                                                                                           >  1:1744057/1‑133 (MQ=255)

                   gagagaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCAATACGACTATACGGGCGTTTTAGGAATTTTTTTTTAAA‑TTTTTTGAGATTTTAATTGCtttt                                                                                                                           >  1:969934/1‑140 (MQ=255)

                   gagagaAGGAGCAACTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTTTAG‑TTTTGGGAGATTTGAATGGCtttt                                                                                                                           >  2:1216644/1‑140 (MQ=255)

                    agagaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTTTTTTTAT‑TTTTTTGAGATTTTAATGGCtttta                                                                                                                          >  1:330485/1‑139 (MQ=255)

                    agagaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTTAAG‑TTTTGGGAGATTTGAATGGCTTTTc                                                                                                                          >  2:593317/1‑140 (MQ=255)

                    agagaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAG‑TTTTGGGAGATTTTAATGGCTTTTc                                                                                                                          >  2:1108621/1‑140 (MQ=255)

                    agagaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAG‑TTTTGGGAGATTTGAATGGCTTTTc                                                                                                                          >  2:765262/1‑140 (MQ=255)

                    agagaAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTGTTCGGATTTTTGTTTGAAG‑TTTTGTGAGATTTGAATGGCTTTTc                                                                                                                          >  1:815188/1‑140 (MQ=255)

                        aaGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTGTTCGGATTTTTGTTTGAAG‑TTTTGGGAGATTTGAATGGGTTTTACGtt                                                                                                                      >  2:1265233/1‑140 (MQ=255)

                              cATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTTTTTGTAG‑TTTTGGGAGATTTTAATGTGTTTTCCGTTTTTaaa                                                                                                                >  1:118271/1‑140 (MQ=255)

                              cATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTTTTTGAAG‑TTTTGGGAGATTTGAATGGCTTTTCCGTTTTTaaa                                                                                                                >  2:1004291/1‑140 (MQ=255)

                              cATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAG‑TTTTTGGCGTTTTGAATGGGGTTTGCGTTTTTtaa                                                                                                                >  1:2125348/1‑140 (MQ=255)

                                tctcTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAATTCGATCATTCCGACTATGCGGGCGTTTTCGGATTTTTGTTTGAAG‑TTTTGGGAGATTTGAATGGCTTTTACGTTTTTaaaaa                                                                                                              >  2:933448/1‑139 (MQ=255)

                                tctcTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTTTTTTAAG‑TTTTTGGAGATTTGAATGGCTTTTACGTTTTTaaaaa                                                                                                              >  2:833501/1‑139 (MQ=255)

                                tctcTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGGTTTCGGATTTTTGTTTCAAG‑TTTTTGGCGTTTTGAATGGCTTTTCCGTTTTTAAAAc                                                                                                              >  2:1376945/1‑140 (MQ=255)

                                tctcTGAGGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAATTCGAGCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAG‑TTTTGGGAGGTTTGAATGGCTGTTACGTTTTTAAAAc                                                                                                              >  2:966129/1‑140 (MQ=255)

                                 ctctGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAG‑TTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACa                                                                                                             <  1:1007029/140‑1 (MQ=255)

                                        gTTGGCATTATTCCGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTGTTCGGATTTTTGTTTGAAG‑TTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAG‑AGGCa                                                                                                     >  2:1308371/1‑140 (MQ=255)

                                          tGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAG‑TTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAG‑AGGCAAg                                                                                                   <  1:1553196/140‑1 (MQ=255)

                                          tGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAG‑TTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAG‑AGGCAAg                                                                                                   <  2:1037380/140‑1 (MQ=255)

                                          tGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAG‑TTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAG‑AGGCAAg                                                                                                   <  1:2212956/140‑1 (MQ=255)

                                          tGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAG‑TTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAG‑AGGCAAg                                                                                                   <  1:222885/140‑1 (MQ=255)

                                          tGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAG‑TTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAG‑AGGCAAg                                                                                                   <  1:1252168/140‑1 (MQ=255)

                                          tGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAG‑TTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAG‑AGGCAAg                                                                                                   <  1:1882434/140‑1 (MQ=255)

                                           ggAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTTTTTTTAG‑TTTTGGGAGATTTTAATGGCTTTTCCGTTTTTAAAAAAAA‑AGGCaaac                                                                                                  >  1:2118955/1‑138 (MQ=255)

                                           ggAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTTTTTTAAT‑TTTTGGGAGATTTGAATGGCGTTTCCGTTTTTAAAAAAAG‑AGGCAAGc                                                                                                  >  1:1082831/1‑140 (MQ=255)

                                           ggAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTTAAG‑TTTTTGGAGATTTTAATTGCTTTTCCGTTTTTAAAAAAAG‑AGGCAAGc                                                                                                  >  2:632780/1‑140 (MQ=255)

                                           ggAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTTAAG‑TTTTGTGAGATTTTAATGGCTTTTCCGTTTTTAAAACAAT‑AGGAAAGc                                                                                                  >  2:2343453/1‑140 (MQ=255)

                                           ggAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAG‑TTTTGGGAGATTTGAATGGCTTTTACGTTTTTAAAACAAG‑AGGCAAGc                                                                                                  >  2:2133697/1‑140 (MQ=255)

                                           ggAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAG‑TTTTGGGAGATTTGAATGGCGTTTCCGTTTTTTAAAAAAG‑ATGCAAGc                                                                                                  >  1:2415417/1‑140 (MQ=255)

                                           ggAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTGTTAGGATTTTTGTTTGTAA‑TTTTGGGAGATTTTAATGTCTTTTACGTTTTTAAAACAAGCAGGCaaa                                                                                                   >  1:40827/1‑139 (MQ=255)

                                           ggAATTATACAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATAAGGGCGTTTTATGATTTTTGTTTGAAG‑TTTTTTGAGATTTAAATAGCGTTTCCGTTTTTAAAACAAA‑AGGCAAGc                                                                                                  >  2:2057305/1‑140 (MQ=255)

                                            gAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTAGGATTTTTGTTTGAAG‑TTTTGGGAGATTTGAATGGGTTTTCCCTTTTTAAAAAAAG‑AGGCAAAcc                                                                                                 >  2:1258815/1‑140 (MQ=255)

                                                 attCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAG‑TTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAG‑AGGCAAGCCAGATa                                                                                            >  2:715317/1‑140 (MQ=255)

                                                 attCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAG‑TTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAG‑AGGCAAGCCAGATa                                                                                            <  2:19687/140‑1 (MQ=255)

                                                 attCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAG‑TTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAG‑AGGCAAGCCAGATa                                                                                            <  2:1562811/140‑1 (MQ=255)

                                                 attCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTAGGATTTTTTTTTTAAG‑TTTTTGGAGATTTTAATTGCTTTTCCGTTTTTAAAACAAG‑AGGCAAGCCAGATa                                                                                            >  2:2161837/1‑140 (MQ=255)

                                                  ttCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAG‑TTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAG‑AGGCAAGCCAGATaa                                                                                           >  2:2162719/1‑140 (MQ=255)

                                                       agaTGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCAGATTTTTGTTTGAAT‑TTTTGGGTGAATTGAATGGCTTGTCCGTTTTTTAAAAAAG‑AGGCAAACCACGTAAAACCg                                                                                      >  1:1264449/1‑140 (MQ=255)

                                                         aTGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAG‑TTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAG‑AGGCAAGCCAGATAAACCCGac                                                                                    >  2:2097444/1‑140 (MQ=255)

                                                           gCTGTTTTTGTATTTTTGTTCTAATTCTAAGTCGATGATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAG‑TTTTGGGAGATTTGAATGTCTTGTCCGTTTTTACAACAAG‑AGGCAAGGCAGATAAACCCGacaa                                                                                  <  2:115797/140‑1 (MQ=255)

                                                                ttttGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAG‑TTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAG‑AGGCAAGCCAGATAAACCCGacaacaaca                                                                             <  1:1881133/140‑1 (MQ=255)

                                                                         tttGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAG‑TTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAG‑AGGCAAGCCAGATAAACCCGACAACAACAAAAaataat                                                                    >  2:2129827/1‑140 (MQ=255)

                                                                             ttCTACTACGAAGTCGCTCAGTCCGACTATCCGGGCGTTTTCGGATTTTTGTTTGAAG‑TTTTGGGAGCTTTGAATCGCTTGGCCGTATTTAAAACAAG‑AGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAg                                                                <  2:1214607/140‑1 (MQ=255)

                                                                             ttCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAG‑TTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAG‑AGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAg                                                                <  2:1353215/140‑1 (MQ=255)

                                                                                 aaTACGAAGTCGATCATTCCGACTATACGGGCGTGTTCGGATTTTTTTTTTTAT‑TTTTTGGAGATTTGAATGTGTTTTGCGTTTTTTAAACAAC‑AGGCAAGCCAGATAAAACCCACACCAACAAAAAATAATCTAGGAAg                                                            >  1:936773/1‑140 (MQ=255)

                                                                                   tACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAG‑TTTTGGGAGATTTGCATGGCTTTTCCGTTTTTAAAACAAG‑AGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTa                                                          <  1:1370430/140‑1 (MQ=255)

                                                                                   tACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAG‑TTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAG‑AGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTa                                                          <  1:1014586/140‑1 (MQ=255)

                                                                                   tACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAG‑TTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAG‑AGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTa                                                          <  1:1347906/140‑1 (MQ=255)

                                                                                   tACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAG‑TTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAG‑AGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTa                                                          <  2:330485/140‑1 (MQ=255)

                                                                                   tACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAG‑TTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAG‑AGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTa                                                          <  1:1665059/140‑1 (MQ=255)

                                                                                   tACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAG‑TTTTGGGAGATGTGAAGGGCTTTTCCGTTTTTAAAACAAG‑AGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTa                                                          <  1:1819418/140‑1 (MQ=255)

                                                                                    aCGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAG‑TTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAG‑AGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTaa                                                         <  2:2398840/140‑1 (MQ=255)

                                                                                    aCGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAG‑TTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAG‑AGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTaa                                                         <  2:40827/140‑1 (MQ=255)

                                                                                    aCGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAG‑TTTTGGGAGATTTGAATGGCTTGTCCGTTTTTAAAACAAG‑AGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTaa                                                         <  2:43362/140‑1 (MQ=255)

                                                                                       aaGTCGATCATTCCGACTATACGGGCGTGTTGGGTTTTTTGTTTTAAG‑TTTTGGGAGATTTGTATGGGGTTTGCGTTTTTATAAAAAG‑AGGCAAGCCCGATAAAACCCACAACAACAAAAAATAATAAATCAAGTaaaag                                                      >  1:898741/1‑138 (MQ=255)

                                                                                        aGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAG‑TTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAG‑AGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTc                                                     <  2:1619305/140‑1 (MQ=255)

                                                                                        aGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAG‑TTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAG‑AGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTc                                                     <  2:17762/140‑1 (MQ=255)

                                                                                        aGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAG‑TTTTGGGAGATTTGAAGGGCTGTTCCGTTTTTAAAACAAG‑AGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTc                                                     <  1:1632338/140‑1 (MQ=255)

                                                                                          tCGATCATTCCGACTATACGGGCGTGGTGGGCTTTTTGTTTGTAGTTTTTGGGGGGTTTTATTGGGTTTGCGGTTTTT‑AAACAAG‑AGGCCACCCACATAAACCCCACAACAACAAAAAATAATATAGCAAGTaaaataca                                                   >  1:303024/1‑135 (MQ=255)

                                                                                          tCGATCATTCCGACTATACGGGCGTGGTGGGATTTTTTTTTTTAG‑TTTTTGGGGCTTTTAATTGCTTTTCCGTTTTTAAAAAAAG‑AGGCAAGCCCCATAAAAACGACCAAAAAAAAAAAAAATATATCAACTaaaaacca                                                   >  1:1578646/1‑135 (MQ=255)

                                                                                           cGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAG‑TTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAG‑AGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCcat                                                  <  2:936773/140‑1 (MQ=255)

                                                                                             aTCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAG‑TTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAG‑AGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCcatgc                                                >  2:2358856/1‑140 (MQ=255)

                                                                                             aTCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAG‑TTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAG‑AGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCcatgc                                                >  2:1595037/1‑140 (MQ=255)

                                                                                              tCATTCCGCCTATACGGGCGTTTTCGGATTTTTGTTTGAAG‑TTTTGGGAGATTTGACTGGCTTTTCCGTTTTTAAAACAAG‑AGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCcatgca                                               <  1:1172244/140‑1 (MQ=255)

                                                                                              tCATTCCGACTATACGGGCTTTTTCGGATTTTTGTTTGAAG‑TTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAG‑AGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCcatgca                                               <  1:2116311/140‑1 (MQ=255)

                                                                                              tCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAG‑TTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAG‑AGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCcatgca                                               <  1:18662/140‑1 (MQ=255)

                                                                                              tCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAG‑TTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAG‑AGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCcatgca                                               <  1:715317/140‑1 (MQ=255)

                                                                                              tCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAG‑TTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAG‑AGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCcatgca                                               <  1:765262/140‑1 (MQ=255)

                                                                                              tCATTCCGACTATACGGGAGTTTTCTGTTTTTTGTATGAAG‑TTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAG‑AGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCcatgca                                               <  1:1124582/140‑1 (MQ=255)

                                                                                              tCAGTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAG‑TTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAG‑AGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCcatgca                                               <  1:2160820/140‑1 (MQ=255)

                                                                                                    cGACTATACGGGCGTTTTCGGATTTTTGTTTGAAG‑TTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAG‑AGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGt                                         <  2:2093272/140‑1 (MQ=255)

                                                                                                           aCGGGCGTGTTCGGATTTTTGTTTGAAG‑TTTTGGGCGATTTGAATGGCTTTTACGTTTTTAAAAAAAG‑AGGCAAGCCCGATAAACCCGACACCAACAAAAAATAATATAGCAAGTAAAATCAATGCATGGGGTAatacat                                  >  2:1503569/1‑140 (MQ=255)

                                                                                                                  ttttCGGATTTTTGTTTTAAG‑TTTTGGGAGATTTTAATTGCTTTTCCGTTTTTAAAAAAAT‑AGGCAATCCAGATAAACCCGACAAAAACAAAAAATAATCAAGCAACTAAAATACAATCATGGGGTAATTAATTCTTTGc                           >  2:1783554/1‑140 (MQ=255)

                                                                                                                  tgttCGGATTTTTGTTTGAAG‑TTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAG‑AGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGc                           >  2:472791/3‑140 (MQ=255)

                                                                                                                   tttCGGATTTTTGTTTGAAG‑TTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAG‑AGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCa                          <  1:2097444/140‑1 (MQ=255)

                                                                                                                    ttCGGATTTTTGTTTGAAG‑TTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAG‑AGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCaa                         <  2:1706014/140‑1 (MQ=255)

                                                                                                                    ttCGGATTTTTGTTTGAAG‑TTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAG‑AGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCaa                         <  1:360172/140‑1 (MQ=255)

                                                                                                                    ttCGGATTTTTGTTTGAAG‑TTTAGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAG‑AGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTACATGCATGGGGTAATTCATTCGTTGCaa                         <  2:1084069/140‑1 (MQ=255)

                                                                                                                    ttCGGATTTTTGTATTAAG‑TTTTGGGAGATTTGAATGGCTTGTCCGTTTTTAAAACAAG‑AGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCaa                         <  2:1676064/140‑1 (MQ=255)

                                                                                                                        gATTTTTGTTTGAAG‑TTTTGGGATATTTGAATGGCTTTTCCGTTTTTAAAACAAG‑AGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCAAAata                     <  2:2223121/140‑1 (MQ=255)

                                                                                                                               gtttgAAG‑TTTTTGGAGATTTGAATGGCTTTTACGTTTTTATAACAAG‑AGGCTAGCCAGATAAACCAGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCAAAATATAAAAAt              <  1:386429/140‑1 (MQ=255)

                                                                                                                               gtttgAAG‑TTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAG‑AGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCAAAATATAAAAAt              <  1:2162719/140‑1 (MQ=255)

                                                                                                                                ttttAAG‑TTTGTGGATTTTTGAATTTGTTTTCAGTTTTTAAAACAAG‑AGGCAAGCAAGTTAAACCCGAAAACAACAAAAAATAATCTAGGAAGTAAAATCCATGCATGGGGTAATTAATTCTTTGCAAAATATAAAAATa             <  2:333794/140‑1 (MQ=255)

                                                                                                                                tttgAAG‑TTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAG‑AGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCAAAATATAAAAATa             <  2:379491/140‑1 (MQ=255)

                                                                                                                                 ttgAAG‑TTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAG‑AGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCAAAATATAAAAATag            <  1:1242124/140‑1 (MQ=255)

                                                                                                                                 ttgAAG‑TTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAG‑AGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCAAAATATAAAAATag            <  1:2209457/140‑1 (MQ=255)

                                                                                                                                   gAAG‑TTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAG‑AGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCAAAATATAAAAATagag          >  1:260208/1‑140 (MQ=255)

                                                                                                                                   gAAG‑TTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAG‑AGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCAAAATATAAAAATagag          >  1:1781538/1‑140 (MQ=255)

                                                                                                                                            gggAGATTTGAATGGCTTTTCCGTTTTTAAAACAAG‑AGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCAAAATATAAAAATAGAGTTACTAAt  <  2:40234/140‑1 (MQ=255)

GTGGAGAAGGTGTAAGAGAGAGAGAAGGAGCATCTCTGATGTTGGAATTATTCAGAGATGCTGTTTTTGTATTTTTGTTCTAATACGAAGTCGATCATTCCGACTATACGGGCGTTTTCGGATTTTTGTTTGAAG‑TTTTGGGAGATTTGAATGGCTTTTCCGTTTTTAAAACAAG‑AGGCAAGCCAGATAAACCCGACAACAACAAAAAATAATCTAGCAAGTAAACTCCATGCATGGGGTAATTCATTCGTTGCAAAATATAAAAATAGAGTTACTAAT  >  NZ_CP034943/240059‑240337

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`