BRESEQ :: Evidence

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	2,991,236	0	C	G	27.8%	386.3 / 52.4	208	intergenic (+37/‑170)	gbsR/bslA	transcriptional regulator GbsR/biofilm‑surface layer protein BslA
Reads supporting (aligned to +/- strand): ref base C (14/113); new base G (49/0); total (94/114)
Fisher's exact test for biased strand distribution p-value = 3.46e-31
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 211 total aligned reads displayed.

GCAACTGGAAAGCTCTAAAGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGT‑TTTTTTGTTCTTTTTTATG‑TAA‑TTACTAC‑AAATTTGAAAAATACG‑GGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTA‑TGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCGGGCAGGCCTGAATA  >  NZ_CP034943/2991097‑2991375

    cTGGAAAGCTCTAAAGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGT‑GTTTTTGTtttttt                                                                                                                                              >  2:1052609/1‑140 (MQ=255)

     tGGAAAGCTCTAAAGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGT‑GTTTTTGTTGttttt                                                                                                                                             >  1:3761149/1‑140 (MQ=255)

         aaGCTCTAAAGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGT‑GTTTTTGTTTTTTTTTAtt                                                                                                                                         >  2:1815285/1‑139 (MQ=255)

           gCTCTAAAGCTTACTATGTTTGGCTTACACGGCTTGCTGAATCGGTGCAGAGCGGGTAAATTTTTAAATTTATACCGATTTAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGT‑TTTTTTGTTCTTTTTTATG‑Ta                                                                                                                                      <  1:1595629/140‑1 (MQ=255)

           gCTCTAAAGATTACTATGATTGGCTGAAACGGCTTGCTGACTCGGTGCAGCGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGT‑TTTTTTGTTCTTTTTTATG‑Ta                                                                                                                                      <  1:3971612/140‑1 (MQ=255)

            ctctAAAGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTCTACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGT‑GGTTTGGTTGTTTTTTATG‑Tat                                                                                                                                     >  1:659765/1‑139 (MQ=255)

            ctctAAAGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGT‑TTTTTTTTTGTTTTTTATT‑Taa                                                                                                                                     >  2:228634/1‑140 (MQ=255)

               tAAAGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGT‑GTTTTTGTTTTTTTTTTTT‑tta‑tta                                                                                                                                 >  1:1907629/1‑140 (MQ=255)

               tAAAGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGT‑GTTTGTGTTATTTTTTATG‑TAA‑Tta                                                                                                                                 >  2:1759764/1‑140 (MQ=255)

               tAAAGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGACTGGGAAACCTGGCGT‑GTTTTTGTTGTTTTTTATG‑tgagaa                                                                                                                                  >  1:1648495/1‑135 (MQ=255)

                 aaGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGT‑TTTTGTGTTGTTTTTTATG‑TAA‑Ttaat                                                                                                                               >  2:4184186/1‑138 (MQ=255)

                      tACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGTTTTTTTTTTTTTTTTTTT‑TAA‑Ttaaaaaaa                                                                                                                           >  1:1267576/1‑134 (MQ=255)

                      tACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGT‑TTTTTTGTTGTTTTTTTTT‑TAA‑TTAATAC‑aaa                                                                                                                         >  1:2772230/1‑140 (MQ=255)

                      tACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGT‑GTTTTTTTTGTTTTTTTTT‑TTA‑TTAATAa‑aaa                                                                                                                         >  1:1187797/1‑140 (MQ=255)

                       aCTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGT‑GTTTTTTTTGTTTTTTTTT‑TAA‑ATAATAa‑aaaa                                                                                                                        >  1:3529601/1‑139 (MQ=255)

                       aCTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGT‑GTTTTTTTTGTTTTTTTTGTTAA‑Ttataaaaaa                                                                                                                          >  1:2136569/1‑133 (MQ=255)

                       aCTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGT‑GTTTTTGTTATTTTTTATT‑TAA‑TTAATAC‑AAAt                                                                                                                        >  2:2006864/1‑140 (MQ=255)

                          attaTTGGCTTAAACGGGTTTATGAATGGGTGCACAGCGGGTAAATTTTTAAATTTATACCTATTGAAACAAATTAAAACAAAAACGCCTGGGAAACCGGGCGT‑TTTTTTGTTCTTTTTTATG‑TAA‑TTACTAC‑AAATTTg                                                                                                                     <  1:409255/137‑1 (MQ=255)

                          aTGTTTGGCTTAATCGGTTTGCTGAATCGGTGCAGAGCGGGTAAATTTTTAAATTTATACCGATTTAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGT‑TTTTTTGTTCTTTTTTATG‑TAA‑TTACTAC‑AAATTTg                                                                                                                     <  1:872480/140‑1 (MQ=255)

                             aTTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGT‑TTTTTTTTTGTTTTTTATT‑TAA‑TTACTAC‑AAATTTGtaa                                                                                                                  >  2:4012224/1‑140 (MQ=255)

                                 gCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGT‑TTTTTTGTTGTTTTTTATG‑TAA‑TTAATAC‑AAATTTGTAAAATa                                                                                                              >  2:1071007/1‑140 (MQ=255)

                                 gCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGT‑GTTTTTTTTTTGTTTTATT‑TAATTTATTAA‑AAATTTTaaaaaa                                                                                                               >  1:168714/1‑139 (MQ=255)

                                   tGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGT‑TTTTTTGTTGTTTTTTATG‑TAA‑TTAAAAC‑AAAATTTAAAAATAcc                                                                                                            >  2:1597764/1‑139 (MQ=255)

                                     aaccGGCTAGCTCAATCGGTGCAGAGCGGTGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAACACGCCTGGGAAACCGGGCGT‑TTTTTTGTTCTTTTTTATG‑TAA‑TTACGAC‑AACTTTGAAAAATACg‑gg                                                                                                         <  1:3085830/137‑1 (MQ=255)

                                       aCGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCAGGGAAACCGGGCGT‑TTTTTTGTTCTTTTTTATG‑TAA‑TTACTAA‑AAATTTGTAAAAAAAG‑GGaa                                                                                                       >  2:3119017/1‑140 (MQ=255)

                                        cGGCTTGCTGAATCGGTGCCGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGAAAAACAAACACGCCTGGGAAACCGGGCGT‑TTTTTTGTTCTTTTTTATG‑TAA‑TTACTAC‑AAATTTGAAAAATACG‑GGaaa                                                                                                      <  1:2423211/140‑1 (MQ=255)

                                           cTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGTTTTTTTTTTTTTTTTTTT‑TAT‑TTATAAA‑AAATTTAAAAAAAAAG‑Gaaaaaa                                                                                                    >  1:3698670/1‑140 (MQ=255)

                                           cTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGT‑TTTTTTGTTGTTTTTTATT‑TAA‑ATACAAA‑AAATTTGAAAAATAAG‑GGaaaaaa                                                                                                   >  2:629932/1‑139 (MQ=255)

                                           cTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGT‑TTTTTTGTTGTTTTTTATG‑TAA‑TTAATAC‑AAATTTTTAAAATACG‑GGAAAAAc                                                                                                   >  2:4168844/1‑140 (MQ=255)

                                           cTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGT‑GTTTTTTTTTTTTTTTATG‑TAA‑TTAATAC‑AAATTTTAAAAATAAC‑GGACAAAc                                                                                                   >  2:2676319/1‑140 (MQ=255)

                                           cTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGT‑GTTTTTGTTTTTTTTTTTG‑TAA‑TTAATAC‑AAAATTTTAAAATAAA‑GGaaaaaa                                                                                                   >  1:822288/1‑139 (MQ=255)

                                           cTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGT‑GTGTTTGTTCTTTTTTTTT‑TAA‑TTACTAA‑AAAAGTGAAAAATAAG‑GGAAAAAc                                                                                                   >  2:3849116/1‑140 (MQ=255)

                                                    tCGGTGCAGAGCGGGTAAATTTTTAAATTTATACCTATTGAAACAAATTAAAACAAAAACGCCTGGGAAACCGGGCGT‑TTTTTTGTTCTTTTTTATG‑TAA‑TTACTAC‑AAATTTGAAAAATACG‑GGAAAAACATCCCAttt                                                                                          <  1:1052609/140‑1 (MQ=255)

                                                    tCGGTCCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGT‑TTTTTTGTTCTTTTTTATG‑TAA‑TTACTAC‑AAATTTGAAAAATACG‑GGAAAAACATCCCAttt                                                                                          <  1:4018999/140‑1 (MQ=255)

                                                        tGCAGAGCGGGGAAATTTTTAAATTTATACCGAGTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGT‑GTGTTTGTTAGTTTTTATG‑TAA‑TTACATA‑AAATTTTTAAAATTCG‑GGGAAAACATCCCATTTAAtt                                                                                      >  2:2233763/1‑140 (MQ=255)

                                                           agagCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGT‑TTTTTTGTTCTTTTTTATG‑TAA‑TTACTAC‑AAATTTGAAAAATACG‑GGAAAAACATCCCATTTACTTCCa                                                                                   <  1:4120610/140‑1 (MQ=255)

                                                            gcgCGGGTAAATTTTAAAATTTATACCGATTGAAAAAAAGTAAAACAAACACGCCTGGGAAACCGGGCGT‑TTTTTTGTTCTTTTTTATG‑TAA‑TTACTAC‑AAATTTGAAAAATACG‑GGAAAAACATCCCATTTACTTCCaa                                                                                  <  1:366785/138‑1 (MQ=255)

                                                            gcgCGGGCAAATTTTTAAATTTATACCTATTGAAACAAAGTAAAACAAACACGCCTGGGAAACCGGGCGT‑TTTTTTGTTCTTTTTTATG‑TAA‑TTACTAC‑AAATTTGAAAAATACG‑GGAAAAACATCCCATTTACTTCCaa                                                                                  <  1:2289958/138‑1 (MQ=255)

                                                               cGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGT‑GTTTTTGTTCTTTTTTATG‑TTA‑TTTCTAA‑AAATTTGAAAAATACG‑GGAAAAACATCCCAGTTAGTTCCAAAat                                                                               >  2:3160591/1‑140 (MQ=255)

                                                                  ggAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGT‑TTTTGTGTTATTTTTTATT‑TAA‑TTAAAAC‑AAAATTGAAAAATAAC‑GGAAAAAAATCACAATTACATACaaaaattt                                                                            >  2:728329/1‑136 (MQ=255)

                                                                  ggAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGT‑GTTTTTTTTTTGTTTTTTT‑TAA‑TTTAAAA‑AAAATTTAAAAAAAAA‑GGAAAAACAAACCAATTAATTGAaaaaaaat                                                                            >  1:1613393/1‑136 (MQ=255)

                                                                  ggAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGT‑GTTTTTGTTCTTTTTTATG‑TAA‑TTACTAC‑AAAATTGAAAAATAAG‑GGAAAAACATACCCTTTTATTCCAAAATAtt                                                                            >  2:937725/1‑140 (MQ=255)

                                                                  ggAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGT‑GGTTTTTTTGTTTTTTATG‑TAA‑TTAATAC‑AAAATTGAAAAATAAG‑GGAAAAAAATACCAATTAATTCCAAAATAtt                                                                            >  2:556205/1‑140 (MQ=255)

                                                                      aTTTTTAAATTTTTCCATATTTAAAAAAAGACAACCACACACGCCTGGGAAACCGGGCTT‑TTTTTTGTTCTTTTTTATG‑TAA‑TTACTAC‑AAATTTGAAAAATACG‑GGAAAAACATCCCATTTACTTCCAAAATATTTGAt                                                                        <  1:3731503/140‑1 (MQ=255)

                                                                       tttttAATTTTATCCCGATTGAAACAACGTAAAACAAACCCGCCTGGGACACCGGGGGT‑TTTTTTGTTCTTTTTTATG‑TAA‑TTACTAC‑AAATTTGAAAAATACG‑GGAAACACATCCCATTTACTTCCAAAATATTTGATg                                                                       <  2:1774262/140‑1 (MQ=255)

                                                                       tttttAAATTTATCCCGATTCAAACAAAGTAAAACAAACACGCCTGGGAAACCGGGCGT‑TTTTTTGTTCTTTTTTATG‑TAA‑TTACTAC‑AAATTTGAAAAATACG‑GGAAAAACATCCCATTTACTTCCAAAATATTTGATg                                                                       <  1:3119017/140‑1 (MQ=255)

                                                                       tttttAAATTTATACCTATTTAAACAAAGTAAAAAAAAAACGCCTGGGAAACCGGGCGT‑TTTTTTGTTCTTTTTTATG‑TAA‑TTACTAC‑AAATTTGAAAAATACG‑GGAAAAACATCCCATTTACTACCAAAATATTTGATg                                                                       <  2:2136569/140‑1 (MQ=255)

                                                                        ttttAAATTAATACCTATTGAAACAACGTACAACAAAAACGCCTGGGAAACCGGGCGT‑TTTTTTGTTCTTTTTTATG‑TAA‑TTACTAC‑AAATTTGAAAAATACG‑GGAAAAACATCCCATTTACTTCCAAAATATTTGATgg                                                                      <  2:2658777/140‑1 (MQ=255)

                                                                        ttttAAATTAAAACCGATTGAAAAACAGGAAAACACAACCGCCTGGGAAACCGGGCGT‑TTTTTTGTTCTTTTTTATG‑GAA‑TTACTAC‑AAATTTGAAAAATACG‑GCAAAAACATCCCATTTACTTCCAAAATATTTGATgg                                                                      <  1:1343931/140‑1 (MQ=255)

                                                                         tttAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGT‑GTGTTTTTTGGTTTTTTTT‑TTA‑TTAATAG‑AAAATTTAAAAAAAAG‑GGTCAAAAATACCATTTTGTTCAAAAAAAttttgtttt                                                                     >  1:3855240/1‑134 (MQ=255)

                                                                            aaaTTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGT‑TTTTTTGTGGTTTTTTTTT‑TTT‑TTAATAA‑AAATTTTTTAAATAAGTGTAAAAAAAGCCCATTTGGTTAC‑AAATATGTTATGGtgtt                                                                  >  1:428180/1‑137 (MQ=255)

                                                                                      ctaTTGAAAAAAAGTAAAACAACAACGCCTGGGAAACCGGGCGT‑TTTTTTGTTCTTTTTTATG‑TAA‑TTACTAC‑AAATTTGAAAAATACG‑GGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTA‑TGTAgg                                                       <  1:4024543/138‑1 (MQ=255)

                                                                                        aTTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGT‑TTTTTTGTTGTTTTTTATG‑TAA‑TTACTAC‑AAATTTGAAAAATACG‑GGGAAAAAATCCCAATTAATTCCAAAATATTTGATTGTAATTTTT‑tgtatgta                                                     >  2:4086245/1‑140 (MQ=255)

                                                                                        aTTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGT‑GTTTTTTGTGTTTTTTTTG‑TTA‑TTTAAACAAAATTTGAAAAA‑ACG‑GGAAAAAAATACCAGTTACTTGAAAAATATTTTGTTGTAAGTTTT‑TTTTGGTa                                                     >  1:3029948/1‑140 (MQ=255)

                                                                                        aTTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGT‑GGTTTTGTTTTTTTTTATG‑TAA‑TTAATAA‑AAAATTTAAAAAAAAG‑GGAAAAAAAAACCCATTAAGGCAAAAAAATTTGATGTTAATTTTA‑TGTatgga                                                     >  2:1244137/1‑136 (MQ=255)

                                                                                            aaaaaaATTAAAAAAACAACGCCTGGGAAACCGGGCGT‑TTTTTTGTTCTTTTTTATG‑TAA‑TTACTAC‑AAATTTGAAAAATACG‑GGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTA‑TGTAGGTAAAAt                                                 <  1:417783/140‑1 (MQ=255)

                                                                                            aaaaaaAGTAAAACAAAAACGCCTGGGAAACCGGGCGT‑TTTTTTGTTCTTTTTTATG‑TAA‑TTACTAC‑AAATTTGAAAAATACG‑GGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTA‑TGTAGGTAAAAt                                                 <  1:3209671/140‑1 (MQ=255)

                                                                                            aaaaaaAGAAAAACAAAAACGCCTGGGAAACCGGGCGT‑TTTTTTGTTCATTTTTATG‑TAA‑TTACTAC‑AAATTTGAAAAATACG‑GGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTA‑TGTAGGTAAAAt                                                 <  1:626696/140‑1 (MQ=255)

                                                                                            aaaCAAATTAAAACAAAAACGCCTGGGAAACCGGGCGT‑TTTTTTGTTCTTTTTTATT‑TAA‑TTACTAC‑AAATTTGAAAAATACG‑GGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTA‑TGTAGGTAAAAt                                                 <  1:3833521/140‑1 (MQ=255)

                                                                                            aaaCAAATTAAAAAAAAAACGCCTGGGAAACCGGGCGT‑TTTTTTGTTCTTTTTTATG‑TAA‑TTACTAC‑AAATTTGAAAAATACG‑GGAAAAACATCCCATTTACGTCCAAAATATTTGATGGTAATTTTA‑TGTAGGTAAAAt                                                 <  2:1874774/140‑1 (MQ=255)

                                                                                            aaaCAAAGTAAACCAAAAACGCCTGGGAAACCGGGCGT‑TTTTTTGTTCTTTTTTATG‑TAA‑GTACTAC‑AAATTTGAAAAATACG‑GGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTA‑TGTAGGTAAAAt                                                 <  2:1650661/140‑1 (MQ=255)

                                                                                            aaaCAAAGTAAAACAAACACGCCTGGGAAACCGGGCGT‑TTTTTTGTTCTTTTTTATG‑TAA‑TTACTAC‑AAATTTGAAAAATACG‑GGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTA‑TGTAGGTAAAAt                                                 <  2:1190857/140‑1 (MQ=255)

                                                                                                  aGTAAAACAAAAACGCCTGGGAAACCGGGCGT‑GTTTTTGTTGGTTTTTATG‑TAA‑TTTCTAC‑AAATTTGAAAAATAAG‑GGAAAAAAATACCCTTTAATTCACAAATATTTTATGGTTAATTTT‑TGGAGGTAAAAatttttt                                           >  2:2459031/1‑136 (MQ=255)

                                                                                                      acaCAAAAACGCCTGGGAAACCGGGCGT‑TTTTTTGTTCTTTTTTATG‑TAA‑TTACTAC‑AAATTTGAAAAATACG‑GGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTA‑TGTAGGTAAAATTTGTATTTgg                                       <  2:539079/138‑1 (MQ=255)

                                                                                                      aaacaaacACGCCTGGGAAACCGGGCGT‑TTTTTTGTTCTTTTTTATG‑TAA‑TTACTAC‑AAATTTGAAAAATACG‑GGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTA‑TGTAGGTAAAATTTGTATTTgg                                       <  2:3631198/140‑1 (MQ=255)

                                                                                                      aaacaaacACGCCTGGGAAACCGGGCGT‑TTTTTTGTTCTTTTTTATG‑TAA‑TTACTAC‑AAATTTGAAAAATACG‑GGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTA‑TGTAGGTAAAATTTGTATTTgg                                       <  1:470988/140‑1 (MQ=255)

                                                                                                      aaacaaacACGCCTGGGAAACCGGGAGT‑TTTTTTGTTCTATTTTATG‑TAA‑TTACTAC‑AAATTTGACAAAGACG‑GGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTA‑TGTAGGTAAAATTTGTATTTgg                                       <  2:2797549/140‑1 (MQ=255)

                                                                                                      aaaCAACCACGCCTGCGAAACCGGGCGT‑TTTTTTGTTCTTTTTTATG‑TAA‑TTACTAC‑AAATTTGACAAATACG‑GGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTA‑TGTAGGTAAAATTTGTATTTgg                                       <  1:213491/140‑1 (MQ=255)

                                                                                                      aaaCAACAACGCCTGGGAAACCGGGCGT‑TTTTTTGTTCTTTTTTATG‑TAA‑TTACTAC‑AAATTTGAAAAATACG‑GGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTA‑TGTAGGTAAAATTTGTATTTgg                                       <  1:1815285/140‑1 (MQ=255)

                                                                                                      aaaCAAAAACGCCTGGGAAACCGGGCGT‑TTTTTTGTTCTTTTTTATG‑TAA‑TTACTAC‑AAATTTGAAAAATACG‑GGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTA‑TGTAGGTAAAATTTGTATTTgg                                       <  2:2254298/140‑1 (MQ=255)

                                                                                                      aaaCAAAAACGCCTGGGAAACCGGGCGT‑TTTTTTGTTCTTTTTTATG‑TAA‑TTACTAC‑AAATTTGAAAAATACG‑GGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTA‑TGTAGGTAAAATTTGTATTTgg                                       <  1:4072679/140‑1 (MQ=255)

                                                                                                      aaaCAAAAACGCCTGGGAAACCGGGCGT‑TTTTTTGTTCTTTTTTATG‑TAA‑TTACTAC‑AAATTTGAAAAATACG‑GGAAAAACATCCCATTTACTTCCAAAATATTGGATGGTAATTTTA‑TGTAGGTAAAATTTGTATTTgg                                       <  2:605003/140‑1 (MQ=255)

                                                                                                      aaaCAAAAACGCCTGGGAAACCGGGCGT‑TTTTTTGTCCATTTTTATG‑TAA‑TTACTAC‑AAATTTGAAAAATACG‑GGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTA‑TGTAGGTAAAATTTGTATTTgg                                       <  2:495193/140‑1 (MQ=255)

                                                                                                      aaaCAAAAACGCCTGGGAAACCGGGCGT‑GTTTTTTTTGTTTTTTATG‑TTA‑ATAATAC‑AAAATTTAAAAAAAAG‑GGAAAAAAAAACCCATTAATTCAAAAATATTTGTTGGTAATTTTA‑TGTAGGTAAAATTTGTtttttg                                       >  2:3381811/1‑138 (MQ=255)

                                                                                                      aaaCAAAAACGCCTGGGAAACCGGGCGT‑ATTTTTGTTCTTTTTTATG‑TAA‑TTACTAC‑AAATTTGAAAAATACG‑GGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTA‑TGTAGGTAAAATTTGTATTTgg                                       <  1:2663215/140‑1 (MQ=255)

                                                                                                       aaCAAAATCGCCTGGGAAACCGGGCGT‑GTTTTTGTTGTTTTTTATG‑TAA‑TTACTAC‑CAATTTGTAAAATACG‑GGAAAAACATCCCCTTTTCTTCCAAAATATTTGTTGGTAATTTTT‑TGTAGGGACAAGTTTTGTTTggg                                      >  2:3993282/1‑139 (MQ=255)

                                                                                                       aaCAAAAACGCCTGGGAAACCGGGCGT‑TTTTTTTTTATTTTTTATT‑TAA‑TTAATAA‑AAAAATGAAAAATAAG‑GGAAAAAAATCCCAATTTATTACAAAAAATTTTTTTGTAATTTTA‑TGTAGGTAAAATTTTTTTTTgga                                      >  2:4197965/1‑139 (MQ=255)

                                                                                                       aaCAAAAACGCCTGGGAAACCGGGCGT‑TTTTTTGTTCTTTTTTATT‑TAA‑TTAATAC‑AAATTTTAAAAATAAG‑GGAAAAACAAACCATTTAATTGCAAAAAAATTTATGGTAATTTTA‑TTTAGGTAAAATTTGTATTTTGc                                      >  2:906359/1‑140 (MQ=255)

                                                                                                       aaCAAAAACGCCTGGGAAACCGGGCGT‑GTTTTTGTTCTTTTTTATG‑TAA‑TTACTAC‑CAATTTTAAAAATACG‑GGAAAAACATCCCCTTTTCTTCCAAAATATTTGATGGTAATGTTA‑TGTAGGTAAAATTTGTATTTGGc                                      >  2:3423678/1‑140 (MQ=255)

                                                                                                         caacaaCGCCTGGGAAACCGGGCGT‑TTTTTTGTTCTTTTTTATG‑TAA‑TTACTAC‑AAATTTGAAAAATACG‑GGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTA‑TGTAGGTAAAATTTGTATTTGGCCg                                    <  1:2680281/140‑1 (MQ=255)

                                                                                                         cAAACACGCCTGGGAAACCGGGCGT‑TTTTTTGTTCTTTTTTATG‑TAA‑TTACTAC‑AAATTTGAAAAATACG‑GGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTA‑TGTAGGTAAAATTTGTATTTGGCCg                                    <  2:3557573/140‑1 (MQ=255)

                                                                                                         aaaaaaCGCCTGGGAAACCGGGCGT‑TTTTTTGTTCTTTTTTATG‑TAA‑TTACTAC‑AAATTTGAAAAATACG‑GGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTA‑TGTAGGTAAAATTTGTATTTGGCCg                                    <  2:306423/139‑1 (MQ=255)

                                                                                                           aaaaCGCCTGGGAAACCGGGCGT‑TTTTTTGTTCTTTTTTATG‑TAA‑TTACTAC‑AAATTTGAAAAATACG‑GGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTA‑TGTAGGTAAAATTTGTATTTGGCCGtt                                  <  1:3208709/140‑1 (MQ=255)

                                                                                                            aaaCGCCTGGGAAACCGGGCGT‑GGTGTTGGTGTTTTTTTTG‑TAA‑TTACTAC‑AAAATTTTAAAATAAG‑GGAAAAACAACCCCTTTACTTCCAAAATATTTGATGGTAATTTTAGTTTGGGAAAAATTTTTTTTTGGcgttg                                  >  2:202437/1‑136 (MQ=255)

                                                                                                              aCGCCTGGGAAACCGGGCGT‑TTTTTTGTTCTTTTTTATG‑TAA‑TTACTAC‑AAATTTGAAAAATACG‑GGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTA‑TGTAGGTAAAATTTGTATTTGGCCGTTCag                               <  1:3493027/140‑1 (MQ=255)

                                                                                                                gCCTGGGAAACCGGGCGT‑TTTTTTGTTCTTTTTTATG‑TAA‑TTACTAC‑AAATTTGAAAAATACG‑GGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTA‑TGTAGGTAAAATTTGTATTTGGCCGTTCagag                             <  1:2877748/140‑1 (MQ=255)

                                                                                                                gCCTGGGAAACCGGGCGT‑TTTTTTGTTCTTTTTTATG‑TAA‑TTACTAC‑AAATTTGAAAAATACG‑GGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTA‑TGTAGGTAAAATTTGTATTTGGCCGTTCagag                             <  2:2908564/140‑1 (MQ=255)

                                                                                                                cccTGGGAAACCGGGCGT‑TTTTTTGTTCTTTTTTATG‑TAA‑TTACTAC‑AAATTAGAAAAATACG‑GGAAAAACATCCCAGTTACTTCCAAAATATTTGATGGTAATTTTA‑TGTAGGTAAAATTTGTATTTGGCCGTTCagag                             <  2:1471947/139‑1 (MQ=255)

                                                                                                                      gAAACCGGGCGT‑TTTTTTGTTCTTTTTTATG‑TAA‑TTACTAC‑AAATTTGAAAAATACG‑GGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTA‑TGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTg                       <  1:2066134/140‑1 (MQ=255)

                                                                                                                          ccGGGCGT‑TTTTTTGTTATTTTTTATG‑TAA‑TTACTAC‑AAAATTTAAAAATACG‑GGAAAAAAATACCAATTACTTACAAAATAATTGATTGTAATTTTA‑TGTATGTTAAAATTTTATTTTGGCGTTGAGAGGCGGTGaaaa                   >  2:766237/1‑140 (MQ=255)

                                                                                                                               ctt‑ttttttGTTCTTTTTTATG‑TAA‑TTACTAC‑AAATTTGAAAAATACG‑GGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTA‑TGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCggg              <  2:242566/138‑1 (MQ=255)

                                                                                                                               cGT‑TTTTTTGTTCTTTTTTATG‑TAA‑TTACTAC‑AAATTTGAAAAATACG‑GGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTA‑TGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCggg              <  1:1443048/140‑1 (MQ=255)

                                                                                                                               cGT‑TTTTTTGTTCTTTTTTATG‑TAA‑TTACTAC‑AAATTTGAAAAATACG‑GGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTA‑TGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCggg              <  1:1111652/140‑1 (MQ=255)

                                                                                                                               cGT‑TTTTTTGTTCTTTTTTATG‑TAA‑TTACTAC‑AAATTTGAAAAATACG‑GGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTA‑TGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCggg              <  2:3698670/140‑1 (MQ=255)

                                                                                                                               cGT‑TTTTTTGTTCTTTTTTATG‑TAA‑TTACTAC‑AAATTTGAAAAATACG‑GGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTA‑TGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCggg              <  2:1698629/140‑1 (MQ=255)

                                                                                                                               agT‑TTTTTTGTTCTTTTTTATG‑TAA‑TTACTAC‑AAATTTGAAAAATACG‑GGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTA‑TGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCggg              <  2:4226015/139‑1 (MQ=255)

                                                                                                                                tt‑ttttttGTTCTTTTTTATG‑TAA‑TTACTAC‑AAATTTGAAAAATACG‑GGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTA‑TGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCGGGc             <  2:139612/139‑1 (MQ=255)

                                                                                                                                gT‑TTTTTTGTTCTTTTTTATG‑TAA‑TTACTAC‑AAATTTGAAAAATACG‑GGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTA‑TGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCGGGc             <  2:1993105/140‑1 (MQ=255)

                                                                                                                                           tCTTTTTTATG‑TAA‑TTACTAC‑AAATTTGAAAAATACG‑GGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTA‑TGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCGGGCAGGCCTGAat   <  1:728329/140‑1 (MQ=255)

                                                                                                                                            cTTTTTTATG‑TAA‑TTACTAC‑AAATTTGAAAAATACG‑GGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTA‑TGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCGGGCAGGCCTGAata  >  2:4069523/1‑140 (MQ=255)

GCAACTGGAAAGCTCTAAAGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGT‑TTTTTTGTTCTTTTTTATG‑TAA‑TTACTAC‑AAATTTGAAAAATACG‑GGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTA‑TGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCGGGCAGGCCTGAATA  >  NZ_CP034943/2991097‑2991375

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`