BRESEQ :: Evidence

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	2,991,252	0	C	A	29.8%	166.2 / 19.5	105	intergenic (+53/‑154)	gbsR/bslA	transcriptional regulator GbsR/biofilm‑surface layer protein BslA
Reads supporting (aligned to +/- strand): ref base C (20/53); new base A (31/0); total (52/53)
Fisher's exact test for biased strand distribution p-value = 2.77e-13
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 108 total aligned reads displayed.

TACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAA‑AACGCCTGGGAAACCGGGCGTTTTTTTGTT‑CTTTTTTATGT‑AATTACTAC‑AAA‑TTTGAAAAATACGGG‑AAAAAC‑ATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCGGGCAGGCCTGAATATTTTTATAACTTTTTA  >  NZ_CP034943/2991119‑2991391

tACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAA‑AACGCCTGGGAAACCGGGCGTTTTTTTTTT‑GTTTTTTATTT‑AAATAATAC‑aaa                                                                                                                                          >  2:1924537/1‑140 (MQ=255)

tACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAA‑AACGCCTGGGAAACCGGGCGTTTTTTTTTT‑ATTTTTTATTT‑AATTAATAa‑aaa                                                                                                                                          >  2:584730/1‑140 (MQ=255)

tACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAA‑AACGCCTGGGAAACCGGGCGTTTTTTTGTT‑GTTTTTTATGT‑AATTTATAC‑aaa                                                                                                                                          >  2:1905951/1‑140 (MQ=255)

tACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAA‑AACGCCTGGGAAACCGGGCGTGTGTTTTTT‑CTTTTTTTTTT‑AATTACaaa‑aaa                                                                                                                                          >  1:1225144/1‑140 (MQ=255)

tACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATAACGATTGAAACAAAGTAAAACAAA‑AACGCCTGGGAAACCGGGCGTTTTTTTTTT‑ATTTTTTTTGT‑AATTAATAC‑aaa                                                                                                                                          >  2:1902349/1‑140 (MQ=255)

 aCTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAA‑AACGCCTGGGAAACCGGGCGTGTTTTTTTT‑ATTTTTTATTT‑AAATAAAAC‑aaaa                                                                                                                                         >  2:2737643/1‑139 (MQ=255)

 aCTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAA‑AACGCCTGGGAAACCGGGCGTGGTTTTGTT‑ATTTTTTATTT‑TATTAATAa‑aaaa                                                                                                                                         >  2:2527910/1‑139 (MQ=255)

   tATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAA‑AAAGCCTGGGAAACCGGGCGTTTTTTTTTT‑GGTTTTTATGT‑AAATACTAA‑AAA‑ttt                                                                                                                                      >  2:1436082/1‑140 (MQ=255)

       aTTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAA‑AACGCCTGGGAAACCGGGCGTTTTTTTTTT‑ATTTTTTATGT‑AATTACTAA‑AAA‑TTTTaaa                                                                                                                                  >  2:1091654/1‑140 (MQ=255)

       aTTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAA‑AACGCCTGGGAAACCGGGCGTTTTTTTGTT‑ATTTTTTATTT‑AATTAATAA‑AAA‑TTTTaaa                                                                                                                                  >  2:1782778/1‑140 (MQ=255)

       aTTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAA‑AACGCCTGGGAAACCGGGCGTGTTTTTGGT‑GTTTTTTTTTT‑AATTAATAC‑AAA‑TTTGaaa                                                                                                                                  >  1:2617302/1‑140 (MQ=255)

        ttGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAA‑AACGCCTGGGAAACCGGGCGTTTTTTTTTT‑GTTTTTTTTTT‑AATTTCTAA‑AAA‑ATTTaaaa                                                                                                                                 >  1:1775109/1‑140 (MQ=255)

        ttGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAA‑AACGCCTGGGAAACCGGGCGTTTTTTTGTT‑CTTTTTTATTT‑AATTAATAA‑AAA‑tttttaaa                                                                                                                                 >  2:1017293/1‑135 (MQ=255)

        ttGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAA‑AACGCCTGGGAAACCGGGCGTGTTTTTTTT‑GTTTTTTATTT‑AATTAATAA‑AAA‑TTTTaaaa                                                                                                                                 >  1:3037669/1‑140 (MQ=255)

        ttGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAA‑AACGCCTGGGAAACCGGGCGTGTTTTTGTT‑TTTTTTTTTTT‑TAGTAAAAC‑AAA‑ATTTaaaa                                                                                                                                 >  1:1008440/1‑140 (MQ=255)

        ttGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAA‑AACGCCTGGGAAACCGGGCGTGGGTTTGTTGTTTTTTTTTGT‑AA‑TGCTAC‑AAA‑ATTGtaaa                                                                                                                                 >  1:1925375/1‑140 (MQ=255)

             tcaaaCGGCTTGCTGACTCGGTGCCGAGGGGGGAAATTTTTAAATTTATACCGATTTAAACAAAGTAAAACAAA‑CACGCCTGGGAAACCGGGCGTTTTTTTGTT‑CTTTTTTATGT‑AATTACTAC‑AAA‑TTTGAAAAATACg                                                                                                                            <  1:2542844/138‑1 (MQ=255)

                  cGGCTTGCTGATTCGGTGAAGAGCGGGTAAATTTTTAAATTTTTACCTATTGAAACAATGTAAAACAAA‑CACGCCTGGGAAACCGGGCGTTTTTTTGTT‑CTTTTTTATGT‑AATTACTAC‑AAA‑TTTGAAAAATACGGG‑aaa                                                                                                                      <  1:2520892/140‑1 (MQ=255)

                     tttgtttaaTGGGTGCAGAGCGGTTAAATTTTTAAATTTATACCTATTGAAACAATTAAAAAAAAA‑AACGCCTGGGAAACCGGGCGTTTTTTTGTT‑CTTTTTTATGT‑AATTACTAC‑AAA‑TTTGAAAAATACGGG‑AAAAAc                                                                                                                   <  1:1207431/133‑1 (MQ=255)

                     cTTGCTGAATCGGTGCAGAGCGGTTAAATTTTTAATTTTATACCGATTCAAACAAAGAAAAAAAAA‑CACGCCTGGGAAACCGGGCGTTTTTTTGTT‑CTTTTTTATGT‑AATTACTAC‑AAA‑TTTGAAAAATACGGG‑AAAAAc                                                                                                                   <  1:1231997/140‑1 (MQ=255)

                     cTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAA‑AACGCCTGGGAAACCGGGCGTTTTTTTGTT‑CTTTTTTATGT‑AATTACTAC‑AAA‑TTTGAAAAATACGGG‑AAAAAc                                                                                                                   <  2:1966676/140‑1 (MQ=255)

                     cTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAA‑AACGCCTGGGAAACCGGGCGTGGTTTTGTT‑TGTTTTTATGT‑TATTAAAACAAAA‑TTTAAAAAAAAGGGG‑aaaaa                                                                                                                    >  1:967528/1‑140 (MQ=255)

                        gCTTAATGGTTGCTGAGCGGTTTATTTTTTAAATTTTTACGGATTTAAACATATTAAAACAAA‑CACGCCTGGGAAACCGGGCGTTTTTTTGTT‑CTTTTTTATGT‑AATTCCTAG‑AAA‑TTTGAAAAATACGGG‑AAAAAC‑ATc                                                                                                               <  1:584730/140‑1 (MQ=255)

                              tCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAA‑AACGCCTGGGAAACCGGGCGGTTTTTTGTT‑GTTTTTTATGT‑TATTACTAC‑AAA‑TTTTAAAAATACGGG‑GAAAAA‑ATCCCAatt                                                                                                         >  2:613084/1‑140 (MQ=255)

                                 gTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAA‑AACGCCTGGGAAACCGGGCGTTTTTTTGTT‑CTTTGTTATGT‑AATTAATAA‑AAA‑ATTTAAAAATAAGGG‑AAAAAC‑AAACCATTTACt                                                                                                      >  2:2516010/1‑140 (MQ=255)

                                      gcgCGGGGAAATTTTTAAATTTATACCGATTGAAAAAAAGTAAAACAAA‑CACGCCTGGGAAACCGGGCGTTTTTTTGTT‑CTTTTTTATGT‑AATTACTAC‑AAA‑TTTGAAAAATACGGG‑AAAAAC‑ATCCCATTTACTTCCaa                                                                                                 <  1:2527910/138‑1 (MQ=255)

                                         cGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAA‑AACGCCTGGGAAACCGGGCGTGTTTTTGTT‑TTTTTTTATGT‑AAATAATAC‑CAA‑TTTGTCAAAAAGGGG‑AAAAAA‑ATGCCATTTTGTTACAAAat                                                                                              >  2:1649765/1‑140 (MQ=255)

                                           gggAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAA‑AACGCCTGGGAAACCGGGCGTGTTTTTTTT‑GATTTTTTTGT‑AATTTCGAC‑AAA‑TTTTAAAAATAAGGG‑AAAAAC‑ATGCCCTTTTCTTGCACAatat                                                                                            >  2:2369865/1‑140 (MQ=255)

                                           gggAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAA‑AACGCCTGGGAAACCGGGCGTGTTTTTGTT‑TTTTTTTTTTT‑AATTTCAAA‑AAA‑ATTGTAAAATAAGGG‑AAAAAA‑ATACCAATTACTTCCCAAatat                                                                                            >  2:445251/1‑140 (MQ=255)

                                            ggAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAA‑AACGCCTGGGAAACCGGGCGTTTTTTTTTT‑GTTTTTTATTT‑TATTACTAA‑AAA‑TTTGTAAAATAAGGG‑AAAAAA‑ATACCATTTAATTGCAAAAAAtt                                                                                           >  2:1370404/1‑140 (MQ=255)

                                            ggAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAA‑AACGCCTGGGAAACCGGGCGTTTTTTTGTT‑GTTTTTTATGT‑AATTAATAC‑AAA‑TTTGAAAAATACGGG‑AAAAAA‑AAAACATTTAATTACAAAATAtt                                                                                           >  2:2560471/1‑140 (MQ=255)

                                            ggAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAA‑AACGCCTGGGAAACCGGGCGTTTTTTTGTT‑CTTTTTTATTT‑AATTACTAA‑AAA‑TTTTAAAAATACGGG‑GAAAAA‑GTGCCATTTAGTTCCAAAAtttt                                                                                           >  2:3048997/1‑140 (MQ=255)

                                            ggAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAA‑AACGCCTGGGAAACCGGGCGTGTTTTTTTT‑CTTTTTTATTT‑AATTAATAC‑AAA‑TATTAAAAATACGGG‑AAAAAC‑ATCCCAATTAATTCAAAAATAtt                                                                                           >  2:2569753/1‑140 (MQ=255)

                                            ggAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAA‑AACGCCTGGGAAACCGGGCGTGTTTTTTGT‑GTTTTTTGTGT‑AATTTCTAC‑AAA‑GTTGGAAAAAACGGG‑AACAAA‑ATCCCAATTACTTCCAAAAtttt                                                                                           >  2:923355/1‑140 (MQ=255)

                                            ggAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAA‑AACGCCTGGGAAACCGGGCGTGTTTTTGTT‑GTTTTTTTTTT‑AATTTCAAA‑AAA‑AATTAAAAAAAAAGG‑TAAAAC‑AAAACAATTAATTGCAAAAAAtt                                                                                           >  2:2341309/1‑140 (MQ=255)

                                            ggAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAA‑AACGCCTGGGAAACCGGGCGTGTTTTTGTT‑CTTTTTTTTGT‑AATTAATAC‑AAA‑ATTTAAAAATACGGG‑AAAAAC‑ATACCAATTAATTGAAAAataat                                                                                           >  2:338643/1‑138 (MQ=255)

                                            ggAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAA‑AACGCCTGGGAAACCGGGCGTGTTTTTGTT‑CTTTTTTATTT‑AATTAATAA‑AAA‑TTTTAAAAATAAGGG‑AAAAAC‑ATCCAATTTAATTAAAAAATAtt                                                                                           >  2:1824815/1‑140 (MQ=255)

                                            ggAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAA‑AACGCCTGGGAAACCGGGCGTGTTTTTGGT‑GTTTTTTATGT‑TATTACTAC‑AAA‑TTTTTAAAATAAGGG‑AAAAACAAACCCATTAATTGCCaaaaatt                                                                                            >  2:1094487/1‑137 (MQ=255)

                                            ggAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAA‑AACGCCTGGGAAACCGGGCGTGTTGTTGTT‑GTTTTTTATGT‑AATTAATAC‑AAA‑ATTGAAAAATAACGC‑AAAAAC‑ATACCAATTAATTACAAAataat                                                                                           >  2:1045809/1‑138 (MQ=255)

                                            ggAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAA‑AACGCCTGGGAAACCGGGCGTGTGTTTTTT‑GTTTTTTTTGG‑AATTAATAC‑AAA‑TTTGAAAAATCAGGG‑CAAAAC‑AGCCCATTTACTTCCAAAatagt                                                                                           >  2:1137169/1‑138 (MQ=255)

                                            ggAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAA‑AACGCCTGGGAAACCGGGCGTGGTTTTTTT‑CTTTTTTATGT‑AATTACTAA‑AAA‑TTTGGAAAATACGGGCAAAAAA‑AACCCA‑TTAATTCCAAAAAAtt                                                                                           >  2:77422/1‑140 (MQ=255)

                                            ggAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAA‑AACGCCTGGGAAACCGGGCGTGGTTTTGTT‑GTTTTTTTTTT‑TATTTATACAAAA‑TTTTAAAAATAGGGG‑AAAAAA‑ACCCCA‑TTAGTTTCAAAAAAtt                                                                                           >  1:1506098/1‑140 (MQ=255)

                                            ggAAATTTTTAAATTTATACAGATTGAAACAAAGTAAAACAAA‑AACCACTGGGAAAACTGGCGTTTTTTTTTA‑GTCTTTTATTT‑AAATAATAA‑AAA‑TAAGTAAAAAACCGT‑CAAAAC‑CACCCATATAATTACAAAataaa                                                                                           >  2:1191774/1‑138 (MQ=255)

                                                  ttttAAATTTATACCTTTTGAAACAAAGTAAAACAAA‑CACGCCTGGGAAACCGGGCGTTTTTTTGTT‑CTTTTTTATGT‑AATTACTAC‑AAA‑TTTGAAAAATACGGG‑AAAAAC‑ATCCCATTTACTTCCAAAATATTTGATgg                                                                                     <  1:1559438/140‑1 (MQ=255)

                                                                 gATGGAAACAAAGCAAACCAAA‑CACGCCTGCGAAACCGGGCGTATTTTTGTT‑CTTTTTTATGT‑AAGTACTAC‑AAA‑TTTGAAAAATACGGG‑AAAAAC‑ATCCCATTTCCTTCCAAAATATTTGATGGTAATTTTATGTAGGt                                                                      <  1:2331723/140‑1 (MQ=255)

                                                                 catacaaaCACAGTAAAACAAA‑CACGCCTGGGAAACCGGGCGTTTTTTTGTT‑CTTTTTTATGT‑AATTACTAC‑AAA‑TTTGAAAAATACGGG‑AAAAAC‑ATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGt                                                                      <  1:2052165/135‑1 (MQ=255)

                                                                  aTTGAAACAAAGTAAAACAAA‑AACGCCTGGGAAACCGGGCGTGTTTTTTTT‑TGTTTTTTTGT‑ATATAGTAC‑AAAGTTTGAAAAAAACGGA‑CAAACA‑AGCCCATTT‑CGTAAAAAAAATTTTTTGGTAATTTTTTTTTGGTa                                                                     >  1:2179107/1‑140 (MQ=255)

                                                                  aTTGAAACAAAGTAAAACAAA‑AACGCCTGGGAAACCGGGCGTGTTTTTTTT‑GTTGTTTATTT‑AATTAAAAC‑AAA‑AATGTAAAATAAGGG‑AAAAAC‑ATAACATTTAGTTACCAAAAATTTTATGGGAATGTTATGTAGGTa                                                                     >  2:862871/1‑140 (MQ=255)

                                                                  aTTGAAACAAAGTAAAACAAA‑AACGCCTGGGAAACCGGGCGTGTTTTTGTT‑CTTTTTTATGT‑AATTAATAC‑CAA‑TTTTAAAAATACGGG‑AAAAAC‑ATACCAATTAATTCCAAAATATTTGATGGTAATTTTATGTAGGTa                                                                     >  2:2895685/1‑140 (MQ=255)

                                                                    tGAAACAAAGTAAAACAAA‑AACGCCTGGGAAACCGGGCGTGTTTTTGTT‑ATTTTTTATTT‑AAATAATAC‑AAA‑ATTGAAAAATAAGGG‑AAAAAC‑ATACCAATTAAATACAAAAAAATTTATTGTAATTTTATTTATGTaaa                                                                   >  2:1166562/1‑140 (MQ=255)

                                                                    tGAAACAAAGTAAAACAAA‑AACGCCTGGGAAACCGGGCGGTGTTTTTTT‑GTTTTTTATTT‑AATAAAAAA‑AAA‑TATTAAAAATAACGG‑AAAAAA‑AAAACAATTACATAAAAAATATATTATTGTAATTTTtagtagcttaa                                                                   >  2:1841580/1‑135 (MQ=255)

                                                                      aaaaaaAGTAAAACAAA‑AACGCCTGGGAAACCGGGCGTTTTTTTGTT‑CTTTTTTATGT‑AATTACTAC‑AAA‑TTTGAAAAATACGGG‑AAAAAC‑ATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAAt                                                                 <  2:2100927/140‑1 (MQ=255)

                                                                      aaaCAAAGTAAAACAAA‑AACGCCTGGGACACCGGGCGTTTTTTTGTT‑TTTTTTTATGTAAATTACAAA‑AAA‑TTT‑AAAAAAACCGG‑AAAAAC‑ATACCCTTTTATTCCAGTAAAATTTTTGGTAATTTTTtgtatgtagatt                                                                 >  2:2628833/1‑136 (MQ=255)

                                                                      aaaCAAAGTAAAACAAA‑AACGCCTGGGAAACCGGGCGTTTTTTTGTT‑CTTTTTTATGT‑AATTACTAC‑AAA‑TTTGAAAAATACGGG‑AAAAAC‑ATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAAt                                                                 <  2:1506098/140‑1 (MQ=255)

                                                                      aaaCAAAGTAAAACAAA‑AACGCCTGGGAAACCGGGCGTTTTTTTGTT‑CTTTTTTATGT‑AATTACTAC‑AAA‑TTTGAAAAATACGGG‑AAAAAC‑ATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAAt                                                                 <  2:1952345/140‑1 (MQ=255)

                                                                           aactAAAACAAA‑CACGCCTGGGAAACCGGGCGTTTTTTTGTT‑CTTTTTTATGT‑AATTACTAC‑AAA‑TTTGAAAAATACGGG‑AAAAAC‑ATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTa                                                            <  1:445251/137‑1 (MQ=255)

                                                                           aaGTAAAACAAA‑AACGCCTGGGAAACCGGGCGTGTTTTTTTT‑GTTTTTTTTTT‑TATTTATAC‑AAA‑ATTTTAAAATAAAGG‑AAAAAA‑AAACCAATTAAATACAAAAAAATTTTTTGTAATTTTATTTTGTTTAAAtttttt                                                            >  1:1558298/1‑137 (MQ=255)

                                                                            aGTAAAACAAA‑AACGCCTGGGAAACCGGGCGTGTGTTTGTT‑GTTTTTTATGT‑AATTTATAA‑AAA‑ATTGTAAAATACTGG‑CAAAAC‑ATCACAATTTATTGCAAAATATTTGTTGGTTATTTTATTGTTGTAAAAttttttt                                                           >  2:1550866/1‑136 (MQ=255)

                                                                                aaccAAA‑CACGCCTGGGAAACCGGGCGTTTTTTTGTT‑CTTTTTTATGT‑AATTACTCC‑AAA‑TTTGAAAAATACGGG‑AAAAAC‑ATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                                                       <  2:2224377/137‑1 (MQ=255)

                                                                                aaacaaa‑cACGCCTGGGAAACCGGGCGTTTTTTTGTT‑CTTTTTTATGT‑AATTACTAC‑AAA‑TTTGAAAAATACGGG‑AAAAAC‑ATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                                                       <  2:3037669/140‑1 (MQ=255)

                                                                                aaaCAAA‑ACCGCCTGGGAAACCGGGCGTTTTTTTGTT‑CTTTTTTATGT‑AATTACTAC‑AAA‑TTTGAACAATACGGG‑AAAAAC‑ATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                                                       <  2:2575025/140‑1 (MQ=255)

                                                                                aaaCAAA‑AACGCCTGTGAAACCGGGCGTTTTTTTGTT‑CTTTTTTATGT‑AATTACTAC‑AAA‑TTTGAAAAATACGGG‑AAAAAC‑ATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                                                       <  2:1653208/140‑1 (MQ=255)

                                                                                aaaCAAA‑AACGCCTGGGAAACCGGGCTTTTTTTTGTT‑CTTTTTTATGT‑AATTACTAC‑AAA‑TTTGAAAAATACGGG‑AAAAAC‑ATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                                                       <  2:2790428/140‑1 (MQ=255)

                                                                                aaaCAAA‑AACGCCTGGGAAACCGGGCGTTTTTTTGTT‑CTTTTTTATGT‑AATTACTAC‑AAA‑TTTGAAAAATACGGG‑AAAAAC‑ATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                                                       <  2:498750/140‑1 (MQ=255)

                                                                                aaaCAAA‑AACGCCTGGGAAACCGGGCGTTTTTTTGTT‑CTTTTTTATGT‑AATTACTAC‑AAA‑TTTGAAAAATACGGG‑AAAAAC‑ATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                                                       <  1:2903458/140‑1 (MQ=255)

                                                                                aaaCAAA‑AACGCCTGGGAAACCGGGCGTTTTTTTGTT‑CTTTTTTATGT‑AATTACTAC‑AAA‑TTTGAAAAATACGGG‑AAAAAC‑ATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                                                       <  2:518024/140‑1 (MQ=255)

                                                                                aaaCAAA‑AACGCCTGGGAAACCGGGCGTTTTTTTGTT‑CTTTTTTATGT‑AATTACTAC‑AAA‑TTTGAAAAATACGGG‑AAAAAC‑ATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                                                       <  1:1245469/140‑1 (MQ=255)

                                                                                aaaCAAA‑AACGCCTGGGAAACCGGGCGTTTTTTTGTT‑CTTTTTTATGT‑AATTACTAC‑AAA‑TTTGAAAAATACGGG‑AAAAAC‑ATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                                                       <  2:883360/140‑1 (MQ=255)

                                                                                aaaCAAA‑AACGCCTGGGAAACCGGGCGTTTTTTTGTT‑CTTTTTTATGT‑AATTACTAC‑AAA‑TTTGAAAAATACGGG‑AAAAAC‑ATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                                                       <  2:1956887/140‑1 (MQ=255)

                                                                                aaaCAAA‑AACGCCTGGGAAACCGGGCGTTTTTTTGTT‑CTTTTTTATGT‑AATTACTAC‑AAA‑TTTGAAAAATACGGG‑AAAAAC‑ATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                                                       <  2:2338632/140‑1 (MQ=255)

                                                                                aaaCAAA‑AACGCCTGGGAAACCGGGCGTTTTTTTGTT‑CTTTTTTATGT‑AATTACTAC‑AAA‑TTTGAAAAATACGGG‑AAAAAC‑ATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                                                       <  2:2873081/140‑1 (MQ=255)

                                                                                 aacaaacaACCCCTGGGAAACCGGGCGTTTTTTTGTT‑CATTTTTATGT‑AATTACTAC‑AAA‑TTTGAAAAATACGGG‑AAAAAC‑ATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                                                       <  1:868365/140‑1 (MQ=255)

                                                                                     aa‑aaCGCCTGGGAAACCGGGCGTTTTTTTGTT‑CTTTTTTATGT‑AATTACTAA‑AAA‑TTTGAAAAATACGGG‑AAAAAC‑ATCCCATTTACTTCCAAAATATTTGATGGTATTTTTATTTATGTAAAATTTGTAttttggagtt                                                  <  2:907168/140‑8 (MQ=255)

                                                                                           gCCTGGGAAACCGGGCGTTTTTTTGTT‑CTTTTTTATGT‑AATTACTAC‑AAA‑TTTGAAAAATACGGG‑AAAAAC‑ATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCagag                                             <  1:2737643/140‑1 (MQ=255)

                                                                                           gCCTGGGAAACCGGGCGTTTTTTTGTT‑CTTTTTTATGT‑AATTACTAC‑AAA‑TTTGAAAAATACGGG‑AAAAAC‑ATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCagag                                             <  1:2156822/140‑1 (MQ=255)

                                                                                           gCCTGGGAAACCGGGCGTTTTTTTGTT‑CTTTTTTATGT‑AATTACTAC‑AAA‑TTTGAAAAATACGGG‑AAAAAC‑ATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCagag                                             <  1:2858223/140‑1 (MQ=255)

                                                                                           gCCTGGGAAACCGGGCGTTTTTTTGTT‑CTTTTTTATGT‑AATTACTAC‑AAA‑TTTGAAAAATACGGG‑AAAAAC‑ATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCagag                                             <  1:1905951/140‑1 (MQ=255)

                                                                                           gCCTGGGAAACCGGGCGTTTTTTTGTT‑CTTTTTTATGT‑AATTACTAC‑AAA‑TTTGAAAAATACGGG‑AAAAAC‑ATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCagag                                             <  2:1578359/140‑1 (MQ=255)

                                                                                           cccTGGGAAACCGGGCGTTTTTTTGTT‑CTTTTTTATTT‑AATTACTAC‑AAA‑TTTGAAAAATACGGG‑AAAAAC‑ATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCagag                                             <  1:2797778/139‑1 (MQ=255)

                                                                                                     ccGGGCGTTTTTTTGTT‑CTTTTTTATGT‑AATTACTAC‑AAA‑TTTGAAAAATACGGG‑AAACAC‑ATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGaaaa                                   <  1:2076040/140‑1 (MQ=255)

                                                                                                     ccGGGCGTTTTTTTGTT‑CTTTTTTATGT‑AATTACTAC‑AAA‑TTTGAAAAATACGGG‑AAAAAC‑ATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGaaaa                                   <  1:2860744/140‑1 (MQ=255)

                                                                                                     ccGGGCGTTTTTTTGTT‑CTTTTTTATGT‑AATTACTAC‑AAA‑TTTGAAAAATACGGG‑AAAAAC‑ATCCCATTTACTACCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGaaaa                                   <  1:1597841/140‑1 (MQ=255)

                                                                                                          cgattaattGTT‑CTTTTTTATGT‑AATTACTCC‑AAA‑TTTGAAAAATACGGG‑CAAAAC‑ATCCCATTTACTTCCAAAATATATGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCggg                              <  1:613084/133‑1 (MQ=255)

                                                                                                          cGTTTTTTTGTT‑CTTTTTTATGT‑AATTACTAC‑AAA‑TTTGAAAAATACGGG‑AAAAAC‑ATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCggg                              <  1:1017293/140‑1 (MQ=255)

                                                                                                          cGTTTTTTTGTT‑CTTTTTTATGT‑AATTACTAC‑AAA‑TTTGAAAAATACGGG‑AAAAAC‑ATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCggg                              <  2:1512767/140‑1 (MQ=255)

                                                                                                          cGTTTTTTTGTT‑CTTTTTTATGT‑AATTACTAC‑AAA‑TTTGAAAAATACGGG‑AAAAAC‑ATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCggg                              <  2:48814/140‑1 (MQ=255)

                                                                                                          cGTTTTTTTGTT‑CTTTTTTATGT‑AATTACTAC‑AAA‑TTTGAAAAATACGGG‑AAAAAC‑ATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCggg                              <  1:1370404/140‑1 (MQ=255)

                                                                                                          cGTTTTTTTGTT‑CTTTTTTATGT‑AATCACTAC‑AAA‑TTTGAAAAATACGGG‑AAAAAC‑ATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCggg                              <  1:147348/140‑1 (MQ=255)

                                                                                                          cGTTTTTTTGTT‑ATTTTTTATGT‑AATTACTAC‑AAA‑TTTGAAAAATACGGG‑AAAAAC‑ATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCggg                              <  1:2706884/140‑1 (MQ=255)

                                                                                                           gTTTTTTTGTT‑CTTTTTTATGT‑AATTACTAC‑AAA‑TTTGAAAAATACGGG‑AAAAAC‑ATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCGGGc                             <  1:1924537/140‑1 (MQ=255)

                                                                                                            tttttttGTT‑CTTTTTTATGT‑AATTACTAC‑AAA‑TTTGAAAAATACGGG‑AAAAAC‑ATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCGGGCa                            <  1:1782778/140‑1 (MQ=255)

                                                                                                             ttttttGTT‑CTTTTTTATGT‑AATTACTAC‑AAA‑TTTGAAAAATACGGG‑AAAAAC‑ATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCGGGCAg                           <  2:889658/140‑1 (MQ=255)

                                                                                                                       cTTTTTTATGT‑AATTACTAC‑AAA‑TTTGAAAAATACGGG‑AAAAAC‑ATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCGGGCAGGCCTCAata                  >  1:1846263/1‑140 (MQ=255)

                                                                                                                          ttttATGT‑AATTACTAC‑AAT‑TGTGAAAAATACGGG‑AAAAAC‑ATCCCATTTACATCCGAAATATTTGATGTGAATTTTATGTAGGTGAAATTTGTAGTTGGCCGTTCAGAGGAGGTGGAAAGCGGGCAGGCCTGaagagtt               >  1:3069402/1‑135 (MQ=255)

                                                                                                                              aTGT‑CATTACTAC‑AAA‑TTTGAACAAGACGGG‑AAAAAC‑ATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCGGGCAGGCCTGAATATTTTtat           <  2:1671075/140‑1 (MQ=255)

                                                                                                                               tGT‑AATTACTAC‑AAA‑TTTGAAAAATACGGG‑AAAAAC‑ATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCGGGCAGGCCTGAATATTTTtata          >  1:1467110/1‑140 (MQ=255)

                                                                                                                                     ttACTAC‑AAA‑TTTGAAAAATACGGG‑AAAAAC‑ATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCGGGCCGGCCTGAATATTTTTATAACttt     >  1:438117/1‑140 (MQ=255)

                                                                                                                                       actac‑AAA‑TTTGAAAAATACGGG‑AAAAAC‑ATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCGGGCAGGCCTGAATATTTTTATAACttttt   >  1:1402905/1‑140 (MQ=255)

                                                                                                                                       actac‑AAA‑TTTGAAAAATACGGG‑AAAAAC‑ATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCGGGCAGGCCTGAATATTTTTATAACttttt   >  1:529590/1‑140 (MQ=255)

                                                                                                                                        ctac‑AAA‑TTTGAAAAATACGGG‑AAAAAC‑ATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCGGGCAGGCCTGAATATTTTTATAACTTTTTa  >  1:2996163/1‑140 (MQ=255)

TACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAA‑AACGCCTGGGAAACCGGGCGTTTTTTTGTT‑CTTTTTTATGT‑AATTACTAC‑AAA‑TTTGAAAAATACGGG‑AAAAAC‑ATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCGGGCAGGCCTGAATATTTTTATAACTTTTTA  >  NZ_CP034943/2991119‑2991391

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`