BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	2,897,849	A→C	6.5%	L106R (CTT→CGT)	EO946_RS14930 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	2,897,849	0	A	C	6.5%	718.3 / 5.7	268	L106R (CTT→CGT)	EO946_RS14930	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (173/65); new base C (2/28); total (175/93)
Fisher's exact test for biased strand distribution p-value = 1.47e-12
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 272 total aligned reads displayed.

CAAGCCGATCCCATTTATCAACATCAGCCCAAAAATCGTATTACCTAACGGTGCCCAGCCATTCTCACCTGTGAAACATAAGGACAGGACCACCTTCCCAACAAGTGTCACATTTAATAGGAATAAACCAAAT‑ACAAGCGACCGCTTCCAGAAAGCAGCCAAAATAAAACCGAGAAAAACCATCATGAAAGCTATTGTTAAAACCGCCGAGACCGGACTGCTTAAAAGCATTTGTTCAAATTCGAGCACTTTTTGCATTTGCAGATGGATCTTCTT  >  NZ_CP034943/2897713‑2897988

cAAGCCGATCCCATTTATCAACATCAGCCCAAAAATCGTATTACCTAACGGTGCCCAGCCATTCTCACCTGTGAAACATAAGGACAGGACCACCTTCCCAACAAGTGTCACATTTAATAGGAATAAACCAAAT‑ACAAGCg                                                                                                                                          >  2:5134129/1‑140 (MQ=255)

 aaGCCGATCCCATTTATCAACATCAGCCCAAAAATCGTATTACCTAACGGTGCCCAGCCATTCTCACCTGTGAAACATAAGGACAGGACCACCTTCCCAACAAGTGTCACATTTAATAGGAATAAACCAAAT‑ACAAGCGa                                                                                                                                         >  1:3840669/1‑140 (MQ=255)

  aGCCGATCCCATTTATCAACATCAGCCCAAAAATCGTATTACCTAACGGTGCCCAGCCATTCTCACCTGTGAAACATAAGGACAGGACCACCTTCCCAACAAGTGTCACATTTAATAGGAATAAACCAAAT‑ACAAGCGAc                                                                                                                                        >  2:2822411/1‑140 (MQ=255)

   gCCGATCCCATTTATCAACATCAGCCCAAAAATCGTATTACCTAACGGTGCCCAGCCATTCTCACCTGTGACACATAAGGACAGGACCACCTTCCCAACAAGTGTCACAGTGAATCCGACTCAACCCAAT‑ACAATCGAcc                                                                                                                                       >  2:3486915/1‑140 (MQ=255)

        tCCCATTTATCAACATCAGCCCAAAAATCGTATTACCTAACGGTGCCCAGCCATTCTCACCTGTGAAACATAAGGACAGGACCACCTTCCCAACAAGTGTCACATTTAATAGGAATAAACCAAAT‑ACAATCGACCGCTTc                                                                                                                                  >  2:2559397/1‑140 (MQ=255)

        tCCCATTTATCAACATCAGCCCAAAAATCGTATTACCTAACGGTGCCCAGCCATTCTCACCTGTGAAACATAAGGACAGGACCACCTTCCCAACAAGTGTCACATTTAATAGGAATAAACCAAAT‑ACAAGCGACCGCTTc                                                                                                                                  >  2:4219421/1‑140 (MQ=255)

        tCCCATTTATCAACATCAGCCCAAAAATCGTATTACCTAACGGTGCCCAGCCATTCTCACCTGTGAAACATAAGGACAGGACCACCTTCCCAACAAGTGTCACAGTTAATAGGAATAAACCAAAT‑ACAAGCGACCGCTTc                                                                                                                                  >  1:174131/1‑140 (MQ=255)

           cATTTATCAACATCAGCCCAAAAATCGTATTACCTAACGGTGCCCAGCCATTCTCACCTGTGAAACATAAGGACAGGACCACCTTCCCAACAAGTGTCACATTTAATAGGAATAAACCAAAT‑ACAAGCGACCGCTTCCAg                                                                                                                               >  1:4275611/1‑140 (MQ=255)

             tttATCAACATCAGCCCAAAAATCGTATTACCTAACGGTGCCCAGCCATTCTCACCTGTGAAACATAAGGACAGGACCACCTTCCCAACAAGTGTCACATTTAATAGGAATAAACCAAAT‑ACAAGCGACCGCTTCCAGaa                                                                                                                             >  2:1531495/1‑140 (MQ=255)

             tttATCAACATCAGCCCAAAAATCGTATTACCTAACGGTGCCCAGCCATTCTCACCTGTGAAACATAAGGACAGGACCACCTTCCCAACAAGTGTCACATTTAATAGGAATAAACCAAAT‑ACAAGCGACCGCTTCCAGaa                                                                                                                             >  1:3948707/1‑140 (MQ=255)

                     cATCAGCCCAAAAATCGTATTACCTAACGGTGCCCAGCCATTCTCACCTGTGAAACATAAGGACAGGACCACCTTCCCAACAAGTGTCACATTTAATAGGAATAAACCAAAT‑ACAAGCGACCGCTTCCAGAAAGCAGCCa                                                                                                                     >  2:1435832/1‑140 (MQ=255)

                     cATCAGCCCAAAAATCGTATTACCTAACGGTGCCCAGCCATTCTCACCTGTGAAACATAAGGACAGGACCACCTTCCCAACAAGTGTCACATTTAATAGGAATAAACCAAAT‑ACAAGCGACCGCTTCCAGAAAGCAGCCa                                                                                                                     >  2:3367098/1‑140 (MQ=255)

                           cccAAAAATCGTATTACCTAACGGTGCCCAGCCATTCTCACCTGTGAAACATAAGGACAGGACCACCTTCCCAACAAGTGTCACATTTAATAGGAATAAACCAAAT‑ACAAGCGACCGCTTCCAGAAAGCAGCCAAAATaa                                                                                                               >  1:4201935/1‑140 (MQ=255)

                           cccAAAAATCGTATTACCTAACGGTGCCCAGCCATTCTCACCTGTGAAACATAAGGACAGGACCACCTTCCCAACAAGTGTCACATTTAATAGGAATAAACCAAAT‑ACAAGCGACCGCTTCCAGAAAGCAGCCAAAATaa                                                                                                               >  1:5038566/1‑140 (MQ=255)

                                                cGGTGCCCAGCCATTCTCACCTGTGAAACATAAGGACAGGACCACCTTCCCAACAAGTGTCACATTTAATAGGAATAAACCAAAT‑ACAAGCGACCGCTTCCAGAAAGCAGCCAAAATAAAACCGAGAAAAACCATCATGa                                                                                          >  2:2062003/1‑140 (MQ=255)

                                                   tGCCCAGCCATTATAACCTGTTAAAAATACGGACAGGACCACCTTCCCAACAAGTGTCAAATTTATTAGGAATAAACCAAAT‑ACAAGCGACCGCTTCCAGAAAGCAGCCAAAATAAAACCGAGAAAAACCATCATGAAAg                                                                                       <  2:4453931/140‑1 (MQ=255)

                                                     cccAGCCATTCTCACCTGTGAAACATAAGGACAGGACCACCTTCCCAACAAGTGTCACATTTAATAGGAATAAACCAAAT‑ACAATCGACCGCTTCCAGAAAGCAGCCAAAATAAAACCGAGAAAAACCATCATGAAAGCt                                                                                     >  1:279574/1‑140 (MQ=255)

                                                     cccAGCCATTCTCACCTGTGAAACATAAGGACAGGACCACCTTCCCAACAAGTGTCACATTTAATAGGAATAAACCAAAT‑ACAAGCGACCGCTTCCAGAAAGCAGCCAAAATAAAACCGAGACAAACCATCATGAAAGCt                                                                                     >  1:4731474/1‑140 (MQ=255)

                                                     cccAGCCATTCTCACCTGTGAAACATAAGGACAGGACCACCTTCCCAACAAGTGTCACATTTAATAGGAATAAACCAAAT‑ACAAGCGACCGCTTCCAGAAAGCAGCCAAAATAAAACCGAGAAAAACCATCATGAAAGCt                                                                                     >  1:4384217/1‑140 (MQ=255)

                                                     cccAGCCATTCTCACCTGTGAAACATAAGGACAGGACCACCTTCCCAACAAGTGTCACATTTAATAGGAATAAACCAAAT‑ACAAGCGACCGCTTCCAGAAAGCAGCCAAAATAAAACCGAGAAAAACCATCATGAAAGCt                                                                                     >  2:2217776/1‑140 (MQ=255)

                                                     cccAGCCATTCTCACCTGTGAAACATAAGGACAGGACCACCTTCCCAACAAGTGTCACATTTAATAGGAATAAACCAAAT‑ACAAGCGACCGCTTCCAGAAAGCAGCCAAAATAAAACCGAGAAAAACCATCATGAAAGCt                                                                                     >  1:4054174/1‑140 (MQ=255)

                                                     cccAGCCATTCTCACCTGTGAAACATAAGGACAGGACCACCTTCCCAACAAGTGTCACATTTAATAGGAATAAACCAAAT‑ACAAGCGACCGCTTCCAGAAAGCAGCCAAAATAAAACCGAGAAAAACCATCATGAAAGCt                                                                                     >  2:213894/1‑140 (MQ=255)

                                                     cccAGCCATTCTCACCTGTGAAACATAAGGACAGGACCACCTTCCCAACAAGTGTCACATTTAATAGGAATAAACCAAAT‑ACAAGCGACCGCTTCCAGAAAGCAGCCAAAATAAAACCGAGAAAAACCATCATGAAAGCt                                                                                     >  1:692476/1‑140 (MQ=255)

                                                     cccAGCCATTCTCACCTGTGAAACATAAGGACAGGACCACCTTCCCAACAAGTGTCACATTTAATAGGAATAAACCAAAT‑ACAAGCGACCGCTTCCAGAAAGCAGCCAAAATAAAACCGAGAAAAACCATCATGAAAGCt                                                                                     >  1:3106744/1‑140 (MQ=255)

                                                     cccAGCCATTCTCACCTGTGAAACATAAGGACAGGACCACCTTCCCAACAAGTGTCACATTTAATAGGAATAAACCAAAT‑ACAAGCGACCGCTTCCAGAAAGCAGCCAAAATAAAACCGAGAAAAACCATCATGAAAGCt                                                                                     >  1:1094681/1‑140 (MQ=255)

                                                     cccAGCCATTCTCACCTGTGAAACATAAGGACAGGACCACCTTCCCAACAAGTGTCACATTTAATAGGAATAAACCAAAT‑ACAAGCGACCGCTTCCAGAAAGCAGCCAAAATAAAACCGAGAAAAACCATCATGAAAGCt                                                                                     >  1:1155374/1‑140 (MQ=255)

                                                     cccAGCCATTCTCACCTGTGAAACATAAGGACAGGACCACCTTCCCAACAAGTGTCACATTTAATAGGAATAAACCAAAT‑ACAAGCGACCGCTTCCAGAAAGCAGCCAAAATAAAACCGAGAAAAACCATCATGAAAGCt                                                                                     >  1:1189167/1‑140 (MQ=255)

                                                     cccAGCCATTCTCACCTGTGAAACATAAGGACAGGACCACCTTCCCAACAAGTGTCACATTTAATAGGAATAAACCAAAT‑ACAAGCGACCGCTTCCAGAAAGCAGCCAAAATAAAACCGAGAAAAACCATCATGAAAGCt                                                                                     >  1:2757288/1‑140 (MQ=255)

                                                     cccAGCCATTCTCACCTGTGAAACATAAGGACAGGACCACCTTCCCAACAAGTGTCACATTTAATAGGAATAAACCAAAT‑ACAAGCGACCGCTTCCAGAAAGCAGCCAAAATAAAACCGAGAAAAACCATCATGAAAGCt                                                                                     >  1:2018288/1‑140 (MQ=255)

                                                     cccAGCCATTCTCACCTGTGAAACATAAGGACAGGACCACCTTCCCAACAAGTGTCACATTTAATAGGAATAAACCAAAT‑ACAAGCGACCGCTTCCAGAAAGCAGCCAAAATAAAACCGAGAAAAACCATCATGAAAGCt                                                                                     >  1:2029509/1‑140 (MQ=255)

                                                     cccAGCCATTCTCACCTGTGAAACATAAGGACAGGACCACCTTCCCAACAAGTGTCACATTGAATAGGAATAAACCAAAT‑ACAAGCGACCGCTTCCAGAAAGCAGCCAAAATAAAACCGAGAAAAACCATCATGAAAGCt                                                                                     >  2:569669/1‑140 (MQ=255)

                                                             ttCTCACCTGTGAAACATAAGGACAGGACCACCTTCCCAACAAGTGTCACATTTAATAGGAATAAACCAAAT‑ACAAGCGACCGCTTCCAGAAAGCAGCCAAAATAAAACCGAGAAAAACCATCATGAAAGCTATTGTTaa                                                                             >  1:1457372/1‑140 (MQ=255)

                                                              tctcACCTTTGAAAAATAAGGACAGGACCACCTTCCAAAAAATTGTCAAATTTAATAGGAATAAACCAAAT‑ACAAGCGACCGCTTCCAGAAAGCAGCCAAAATAAAACCGAGAAAAACCATCATGAAAGCTATTGTTaaa                                                                            <  2:4384217/140‑1 (MQ=255)

                                                               ctcACCTGTGAAACATAAGGACAGGACCACCTTCCCAACAAGTGTCACATTTAATAGGAATAAACCAAAT‑ACAAGCGACCGCTTCCAGAAAGCAGCCAAAATAAAACCGAGAAAAACCATCATGAAAGCTATTGTTaaaa                                                                           >  2:1050917/1‑140 (MQ=255)

                                                                  aCCTGTGAAACATAAGGACAGGACCACCTTCCCAACAAGTGTCACATTTAATAGGAATAAACCAAAT‑ACAAGCGACCGCTTCCAGAAAGCAGCCAAAATAAAACCGAGAAAAACCATCATGAAAGCTATTGTTAAAAccg                                                                        >  1:3058609/1‑140 (MQ=255)

                                                                   ccTGTTAAAAATAAGGACCGGACCACCTTCCAAACACGTTTAAAATTTAATAGTAATAAACAAAAA‑ACACGCGACCGCTTCCAGAAAGCAGCCAAAATAAAACCGAGAAAAACCATCATGAAAGCTATTGTTAAAAccgc                                                                       <  1:1755344/140‑1 (MQ=255)

                                                                   ccTGTGAAACATAAGGACAGGACCACCTTCCCAACAAGTGTCAAATTTAATAGGAATAAACCAAAT‑ACAAGCGACCGCTTCCAGAAAGCAGCCAAAATAAAACCGAGAAAAACCATCATGAAAGCTATTGTTAAAAccgc                                                                       <  2:3895458/140‑1 (MQ=255)

                                                                   ccTGTGAAACATAAGGACAGCACCACCTTCCCAACAAGTGTCAAATTTAATAGGAATAAACCAAAT‑ACACGCGACCGCTTCCAGAAAGCAGCCAAAATAAAACCGAGAAAAACCATCATGAAAGCTATTGTTAAAAccgc                                                                       <  1:4581979/140‑1 (MQ=255)

                                                                      gttaaaCATACGGACAGCACCACCTTCCCAACAAGTGTCACATTTAATAGGAATAAACCAACT‑ACAAGCGACCGCTTCCAGAAAGCAGCCAAAATAAAACCGAGAAAAACCATCATGAAAGCTATTGTTAAAACCGCCga                                                                    <  2:3948707/137‑1 (MQ=255)

                                                                       tgAAACATAAGGACAGGACCACCTTCCCAACAAGTGTCACATTTAATAGGAATAAACCAAAT‑ACAAGCGACCGCTTCCAGAAAGCAGCCAAAATAAAACCGAGAAAAACCATCAAGAAAGCTATTGTTAAAACCGCCgag                                                                   >  2:3106581/1‑140 (MQ=255)

                                                                        taaaCATAAGGACAGGACCACCTTCCCAACAAGTGTCACATTTAATAGGAATAAACCAAAT‑ACAAGCGACCGCTTCCAGAAAGCAGCCAAAATAAAACCGAGAAAAACCATCATGAAAGCTATTGTTAAAACCGCCgaga                                                                  <  2:3058609/139‑1 (MQ=255)

                                                                            cATAAGGACAGGACCACCTTCCCAACAAGTGTCACATTTAATAGGAATAAACCAAAT‑ACAAGCGACCGCTTCCAGAAAGCAGCCAAAATAAAACCGAGAAAAACCATCATGAAAGCTATTGTTAAAACCGCCGAGACCgg                                                              >  1:2443870/1‑140 (MQ=255)

                                                                            cATAAGGACAGGACCACCTTCCCAACAAGTGTCACATTTAATAGGAATAAACCAAAT‑ACAAGCGACCGCTTCCAGAAAGCAGCCAAAATAAAACCGAGAAAAACCATCATGAAAGCTATTGTTAAAACCGCCGAGACCgg                                                              >  1:3850307/1‑140 (MQ=255)

                                                                            cATAAGGACAGGACCACCTTCCCAACAAGTGTCACATTTAATAGGAATAAACCAAAT‑ACAAGCGACCGCTTCCAGAAAGCAGCCAAAATAAAACCGAGAAAAACCATCATGAAAGCTATTGTTAAAACCGCCGAGACCgg                                                              >  1:367057/1‑140 (MQ=255)

                                                                             acacggacaggacCACCTTCCGACACAGTCTCACATCTAATCGGAAT‑AACCAAATAACACGCGACCGCTTCCAGAACGCAGCCAAAATAAAACCGAGAACAACCATCATGAAAGCTATTGTTAAAACCGCCGAGACCGGa                                                             <  1:5132594/136‑1 (MQ=255)

                                                                             aTACGGCCAGGACCACCTTCCCAACAAGTGTCAAATTTAATAGGAATAAACCAAAA‑ACAAGCGACCGCTTCCAGAAAGCAGCCAAAATAAAACCGAGAAAAACCATCATGAAAGCTATTGTTAAAACCGCCGAGACCGGa                                                             <  1:4088745/140‑1 (MQ=255)

                                                                             aTAAGCACAGCACCACCTTCCCAACACGGCTCACATTTAATAGGAAAAAACCAAAT‑ACCCGCGACCGCTTCCAGAAAGCAGCCAAAATAAAACCGAGAAAAACCATCATGAAAGCTATTGTTAAAACCGCCGAGACCGGa                                                             <  1:2990868/140‑1 (MQ=255)

                                                                                 ggacaggacCACCTTCCCAACAAGTGTCACATTTAATAGGAATAAACCAAAT‑ACAAGCGACCGCTTCCAGAAAGCAGCCAAAATAAAACCGAGAAAAACCATCATGAAAGCTATTGTTAAAACCGCCGAGACCGGACTGc                                                         >  1:322056/1‑140 (MQ=255)

                                                                                 ggacaggacCACATTCCCAAAAAGTGTCACATTTAATAGGAATAAACCAAAT‑ACAAGCGACCGCTTCCAGAAAGCAGCCAAAATAAAACCGAGAAAAACCATCATGAAAGCTATTGTTAAAACCGCCGAGACCGGACTGc                                                         >  1:1259988/1‑140 (MQ=255)

                                                                                       gacCACCTTCCCAACAAGTGTCACATTTAATAGGAATAAACCAAAT‑ACAAGCGACCGCTTCCAGAAAGCAGCCAAAATAAAACCGAGAAAAACCATCATGAAAGCTATTGTTAAAACCGCCGAGACCGGa                                                             >  1:3371880/1‑130 (MQ=255)

                                                                                       gacCACCTTCCCAACAAGTGTCACATTTAATAGGAATAAACCAAAT‑ACAAGCGACCGCTTCCAGAAAGCAGCCAAAATAAAACCGAGAAAAACCATCATGAAAGCTATTGTTAAAACCGCCGAGACCGGACTGCTTaaaa                                                   >  2:5165674/1‑140 (MQ=255)

                                                                                       gacCACCTTCCCAACAAGTGTCACATTTAATAGGAATAAACCAAAT‑ACAAGCGACCGCTTCCAGAAAGCAGCCAAAATAAAACCGAGAAAAACCATCATGAAAGCTATTGTTAAAACCGCCGAGACCGGACTGCTTaaaa                                                   >  2:3634684/1‑140 (MQ=255)

                                                                                       gacCACCTTCCCAACAAGTGTCACATTTAATAGGAATAAACCAAAT‑ACAAGCGACCGCTTCCAGAAAGCAGCCAAAATAAAACCGAGAAAAACCATCATGAAAGCTATTGTTAAAACCGCCGAGACCGGACTGCTTaaaa                                                   >  1:916170/1‑140 (MQ=255)

                                                                                       gacCACCTTCCCAACAAGTGTCACATTTAATAGGAATAAACCAAAT‑ACAAGCGACCGCTTCCAGAAAGCAGCCAAAATAAAACCGAGAAAAACCATCATGAAAGCTATTGTTAAAACCGCCGAGACCGGACTGCTTaaaa                                                   >  1:1006842/1‑140 (MQ=255)

                                                                                       gacCACCTTCCCAACAAGTGTCACATTTAATAGGAATAAACCAAAT‑ACAAGCGACCGCTTCCAGAAAGCAGCCAAAATAAAACCGAGAAAAACCATCATGAAAGCTATTGTTAAAACCGCCGAGACCGGACTGCTTaaaa                                                   >  1:138509/1‑140 (MQ=255)

                                                                                       gacCACCTTCCCAACAAGTGTCACATTTAATAGGAATAAACCAAAT‑ACAAGCGACCGCTTCCAGAAAGCAGCCAAAATAAAACCGAGAAAAACCATCATGAAAGCTATTGTTAAAACCGCCGAGACCGGACTGCTTaaaa                                                   >  1:1489745/1‑140 (MQ=255)

                                                                                           accTTCCAAACAAGTCTCACATTTAATAGCAAAAAACAAAAT‑ACACGCGACCGCTTCCAGAAAGCAGCCAAAATAAAACCGAGAAAAACCATCATGAAAGCTATTGTTAAAACCGCCGAGACCGGACTGCTTAAAAGCAt                                               <  2:1424680/140‑1 (MQ=255)

                                                                                            ccTTCCCAACAAGTGTCACATTTAATAGGAATAAACCAAAT‑ACAAGCGACCGCTTCCAGAAAGCCGCCAAAATAAAACCGAGAAAAACCATCATGAAAGCGATTGTTAAAACCGCCGAGACCGGACTGCTTAAAAGCagt                                              >  1:123210/1‑138 (MQ=255)

                                                                                            ccTTCCCAACAAGTGTCACATTTAATAGGAATAAACCAAAT‑ACAAGCGACCGCTTCCAGAAAGCAGCCAAAATAAAACCGAGAAAAACCATCATGAAAGCTATTGTTAAAACCGCCGAGACCGGACTGCTTAAAAGCAtt                                              >  2:2918196/1‑140 (MQ=255)

                                                                                            ccTTCCCAACAAGTGTCACATTTAATAGGAATAAACCAAAT‑ACAAGCGACCGCTTCCAGAAAGCAGCCAAAATAAAACCGAGAAAAACCATCATGAAAGCTATTGTTAAAACCGCCGAGACCGGACTGCTTAAAAGCAtt                                              >  1:4330939/1‑140 (MQ=255)

                                                                                            ccTTCCCAACAAGTGTCACATTTAATAGGAATAAACCAAAT‑ACAAGCGACCGCTTCCAGAAAGCAGCCAAAATAAAACCGAGAAAAACCATCATGAAAGCTATTGTTAAAACCGCCGAGACCGGACTGCTTAAAAGCAt                                               <  2:1234344/139‑1 (MQ=255)

                                                                                            ccTTCCCAACAAGTGTCACATTTAATAGGAATAAACCAAAT‑ACAAGCGACCGCTTCCAGAAAGCAGCCAAAATAAAACCGAGAAAAAACATCATGAAAGCTATTGTTAAAACCGCCGAGACCGGACTGCTTAAAAGCAtt                                              >  2:225255/1‑140 (MQ=255)

                                                                                               tCCCAACAAGTGGCACATTTAATAGGAATCAACCAAAT‑ACAAGCGACCGCTTCCAGAAAGCAGCCAAAATAAAACCGGGACAAACAATCATGAAAGCTATTGTGAAAACCGCCGAGGCGGGACGGCGTACAAGCATTTGt                                           >  2:1969292/1‑140 (MQ=255)

                                                                                                 ccAACAAGTGTCACATTTAATAGGAATAAACCAAAT‑ACAAGCGACCGCTTCCAGAAAGCAGCCAAAATAAAACCGAGAAAAACCATCATGAAAGCTATTGTTAAAACCGCCGAGACCGGa                                                             <  2:77190/120‑1 (MQ=255)

                                                                                                  caacacGTGTCACATTTAATACTAAAAAACCAAAT‑AAAAGCGACCGCTTCCAGAAAGCAGCCAAAATAAAACCGAGAAAAACCATCATGAAAGCTATTGTTAAAACCGCCGAGACCGGACTGCTTAAAAGCATTTGTTCa                                        <  1:1513135/140‑1 (MQ=255)

                                                                                                  caacaaGTGTCACATTTAATAGGAATAAACCAAAT‑ACAAGCGACCGCTTCCAGAAAGCAGCCAAAATAAAACCGAGAAAAACCATCATGAAAGCTATTGTTAAAACCGCCGAGACCGGACTGCTTAAAAGCATTTGTTCa                                        >  2:2599146/1‑140 (MQ=255)

                                                                                                  caacaaGTGTCACATTTAATAGGAATAAACCAAAT‑ACAAGCGACCGCTTCCAGAAAGCAGCCAAAATAAAACCGAGAAAAACCATCATGAAAGCTATTGTTAAAACCGCCGAGACCGGACTGCTTAAAAGCATTTGTTCa                                        >  2:380312/1‑140 (MQ=255)

                                                                                                  caacaaGTGTCACATTTAATAGGAATAAACCAAAT‑ACAAGCGACCGCTTCCAGAAAGCAGCCAAAATAAAACCGAGAAAAACCATCATGAAAGCTATTGTTAAAACCGCCGAGACCGGACTGCTTAAAAGCATTTGTTCa                                        >  2:3453968/1‑140 (MQ=255)

                                                                                                      aaGTGTCACATTTAATAGGAATAAACCAAAT‑ACAAGCGACCGCTTCCAGAAAGCAGCCAAAATAAAACCGAGAAAAACCATCATGAAAGCTATTGTTAAAACCGCCGAGACCGGACTGCTTAAAAGCATTTGTTCAAAtt                                    >  1:5180149/1‑140 (MQ=255)

                                                                                                          ttCACATTTAATAGCAACAAACAAAAT‑ACACGCGACCGCTTCCAGAAAGCAGCCAAAATAAAACCGAGAAAAACCATCATGAAAGCTATTGTTAAAACCGCCGAGACCGGACTGCTTAAAAGCATTTGTTCAAATTCGAg                                <  1:74334/139‑1 (MQ=255)

                                                                                                          gtcaattttAATAGCAATAAACCAAAT‑ACAAGCGACCGCTTCCAGAAAGCAGCCAAAATAACACCGAGAAAAACCATCATGAAAGCTATTGTTAAAACCGCCGAGACCGGACTGCTTAAAAGCATTTGTTCAAATTCGAg                                <  1:4961147/134‑1 (MQ=255)

                                                                                                          gtCACATTTAATAGGAATAAACCAAAT‑CCAAGCGACCGCTTCCAGAAAGCAGCCAAAATAAAACCGAGAAAAACCATCATGAAAGCTATTGTTAAAACCGCCGAGACCGGACTGCTTAAAAGCATTTGTTCAAATTCGAg                                >  2:4713101/1‑140 (MQ=255)

                                                                                                          gtCACATTTAATAGGAATAAACCAAAT‑ACAAGCGACCGCTTCCAGAAAGCAGCCAAAATAAAACCGAGAAAAACCATCATGAAAGCTATTGTTAAAACCGCCGAGACCGGACTGCTTAAAAGCATTTGTTCAAATTCGAg                                <  2:5038566/140‑1 (MQ=255)

                                                                                                          gtCACATTTAATAGCAATAAACCAAAT‑ACAAGCGACCGCTTCCAGAAAGCAGCCAAAATAAAACCGAGAAAAACCATCATGAAAGCTATTGTTAAAACCGCCGAGACCGGACTGCTTAAAAGCATTTGTTCAAATTCGAg                                <  1:2217776/140‑1 (MQ=255)

                                                                                                           tCACATTTAATAGGAATAAACCAAAT‑ACAAGCGACCGCTTCCAGAAAGCAGCCAAAATAAAACCGAGAAAAACCATCATGAAAGCTATTGTTAAAACCGCCGAGACCGGACTGCTTAAAAGCATTTGTTCAAATTCGAGc                               >  2:2462985/1‑140 (MQ=255)

                                                                                                             acaTTTAATAGGAATAAACCAAAT‑ACAAGCGACCGCTTCCAGAAAGCAGCCAAAATAAAACCGAGAAAAACCATCATGAAAGCTa                                                                                    >  1:4172964/1‑85 (MQ=255)

                                                                                                             acaTTTAATAGGAATAAACCAAAT‑ACAAGCGACCGCTTCCAGAAAGCAGCCAAAATAAAACCGAGAAAAACCATCATGAAAGCTa                                                                                    <  2:4172964/85‑1 (MQ=255)

                                                                                                                tttAATAGGAATAAACCAAAT‑ACAAGCGACCGCTTCCAGAAAGCAGCCAAAATAAAACCGAGAACCACCATCATGAAAGCTATTGTTAAAACCGCCGAGACCGGACTGATTAAAAGAATTTGTTCAAATTCGAGCACttt                          >  1:2836225/1‑140 (MQ=255)

                                                                                                                   aaTAGGAATAAACCAAAT‑ACAAGCGACCGCTTCCAGAAAGCAGCCAAAATAAAACCGAGAAAAACCATCATGAAAGCTATTGTTAAAACCGCCGAGACCGGa                                                             >  2:2393436/1‑102 (MQ=255)

                                                                                                                   aaTAGGAATAAACCAAAT‑ACAAGCGACCGCTTCCAGAAAGCAGCCAAAATAAAACCGAGAAAAACCATCATGAAAGCTATTGTTAAAACCGCCGAGACCGGACTGCTTAAAAGCATTTGTTCAAATTCGAGCACTTtttg                       >  1:2271892/1‑140 (MQ=255)

                                                                                                                       gcaaTAAACCAAAA‑ACACGCGACCGCTTCCAGAAAGCAGCCAAAATAAAACCGAGAAAAACCATCATGAAAGCTATTGTTAAAACCGCCGAGACCGGACTGCTTAAAAGCATTTGTTCAAATTCGAGCACTTtttgcatt                   <  2:138509/138‑1 (MQ=255)

                                                                                                                         aaTAAACCAAAT‑ACAAGCGACCGCTTCCAGAAAGCAGCCAAAATAAAACCGAGAAAAACCATCATGAAAGCTATTGTTAAAACCGCCGAGACCGGACTGCTTAAAAGCATTTGTTCAAATTCGAGCACTTtttgcatttg                 >  1:1849282/1‑140 (MQ=255)

                                                                                                                              aCCAAAT‑ACAAGCGACCGCTTCCAGAAAGCAGCCAAAATAAAACCGAGAAAAACCATCATGAAAGCTATTGTTAAAACCGCCGAGACCGGACTGCTTAAAAGCATTTGTTCAAATTCGAGCACTTTTTGCATTTGCAGAt            >  1:2359313/1‑140 (MQ=255)

                                                                                                                                cAAAT‑ACACGCGACCGCTTCCAGAAAGCAGCCAAAATAAAACCGAGAAAAACCATCATGAAAGCTATTGTTAAAACCGCCGAGACCGGACTGCTTAAAAGCATTTGTTCAAATTCGAGCACTTTTTGCATTTGCAGATgg          <  2:4054174/140‑1 (MQ=255)

                                                                                                                                 caaT‑ACAAGCGACCGCTTCCAGAAAGCAGCCAAAATACAACCGAGAAAAACCATCATGAAAGCTATTGTTAAAACCGCCGAGACCGGACTGCTTAAAAGCATTTGTTCAAATTCGAGCACTTTTTGCATTTGCAGATGGa         <  2:4520982/139‑1 (MQ=255)

                                                                                                                                 aaaT‑ACAAGCGACCGCTTCCAGAAAGCAGCCAAAATAAAACCGAGAAAAACCATCATGAAAGCTATTGTTAAAACCGCCGAGACCGGACTGCTTAAAAGCATTTGTTCAAATTCGAGCACTTTTTGCATTTGCAGATGGa         <  2:4803491/140‑1 (MQ=255)

                                                                                                                                 aaaT‑ACAAGCGACCGCTTCCAGAAAGCAGCCAAAATAAAACCGAGAAAAACCATCATGAAAGCTATTGTTAAAACCGCCGAGACCGGACTGCTTAAAAGCATTTGTTCAAATTCGAGCACTTTTTGCATTTGCAGATGGa         <  2:3147130/140‑1 (MQ=255)

                                                                                                                                 aaaT‑ACAAGCGACCGCTTCCAGAAAGCAGCCAAAATAAAACCGAGAAAAACCATCATGAAAGCTATGGTTAAAACCGCCGAGACCGGACTGCTTAAAAGCATTTGTTCAAATTCGAGCACTTTTTGCATTTGCAGATGGa         <  2:3437464/140‑1 (MQ=255)

                                                                                                                                 aaaC‑ACAAGCGACCGCTTCCAGACAGCAGCCAAACTAAAACCGAGAAAAACCATCATGAAAGCTCTTGTTAAAACCGCCGAGACCGGACTGCTTAAAAGCATTTGTTCAAATTCGAGCACTTTTTGCATTTGCAGATGGa         <  1:2559397/140‑1 (MQ=255)

                                                                                                                                  aaaaaaaaGCGACCGCTTACAGAAAGCAGCCAAAATAAAACCGAGAAAAACCATCATGAAAGCTATTGTTAAAAACGCAGAGACCGGACTGCTTACAAGCATTTGTTCTAATTCGAGCACTTTTTGCATTTGCAGATGGa         <  2:2765731/134‑1 (MQ=255)

                                                                                                                                  aaT‑ACAAGCGACCGCTTCCAGAAAGCAGCCAAAATAAAACCGAGAAAAACCATCATGAAAGCTATTGTTAAAACCGCCGAGACCGGACTGCTTAAAAGCATTTGTTCAAATTCGAGCACTTTTTGCATTTGCAGATGGAt        >  2:1697145/1‑140 (MQ=255)

                                                                                                                                   aT‑ACAAGCGACCGCTTCCAGAAAGCAGCCAAAATAAAACCGAGAAAAACCATCATGAAAGCTATTGTTAAAACCGCCGAGACCGGACTGCTTAAAAGCATTTGTTCAAATTCGAGCACTTTTTGCATTTGCAGATgg          >  2:1752758/1‑137 (MQ=255)

                                                                                                                                   aT‑ACAAGCGACCGCTTCCAGAAAGCAGCCAAAATAAAACCGAGAAAAACCATCATGAAAGCTATTGTTAAAACCGCCGAGACCGGACTGCTTAAAAGCATTTGTTCAAATTCGAGCACTTTTTGCATTTGCAGATGGa         <  1:3378101/138‑1 (MQ=255)

                                                                                                                                   aT‑ACAAGCGACCGCTTCCAGAAAGCAGCCAAAATAAAACCGAGAAAAACCATCATGAAAGCTATTGTTAAAACCGCCGAGACCGGACTGCTTAAAAGCATTTGTTCAAATTCGAGCACTTTTTGCATTTGCAGATGGa         <  1:3383377/138‑1 (MQ=255)

                                                                                                                                   aT‑ACAAGCGACCGCTTCCAGAAAGCAGCCAAAATAAAACCGAGAAAAACCATCATGAAAGCTATTGTTAAAACCGCCGAGACCGGACTGCTTAAAAGCATTTGTTCAAATTCGAGCACTTTTTGCATTTGCAGATGGa         >  2:3378101/1‑138 (MQ=255)

                                                                                                                                   aT‑ACAAGCGACCGCTTCCAGAAAGCAGCCAAAATAAAACCGAGAAAAACCATCATGAAAGCTATTGTTAAAACCGCCGAGACCGGACTGCTTAAAAGCATTTGTTCAAATTCGAGCACTTTTTGCATTTGCAGATGGa         >  2:3383377/1‑138 (MQ=255)

                                                                                                                                   aT‑ACAAGCGACCGCTTCCAGAAAGCAGCCAAAATAAAACCGAGAAAAACCATCATGAAAGCTATTGTTAAAACCGCCGAGACCGGACTGCTTAAAAGCATTTGTTCAAATTCGAGCACTTTTTGCATTTGCAGATGGAtc       >  1:1626506/1‑140 (MQ=255)

                                                                                                                                   aT‑ACAAGCGACCGCTTCCAGAAAGCAGCCAAAATAAAACCGAGAAAAACCATCATGAAAGCTATTGTTAAAACCGCCGAGACCGGACTGCTTAAAAGCATTTGTTCAAATTCGAGCACTTTTTGCATTTGCAGATGGAtc       <  2:2343494/140‑1 (MQ=255)

                                                                                                                                   aT‑ACAAGCGACCGCTTCCAGAAAGCAGCCAAAATAAAACCGAGAAAAACCATCATGAAAGCTATTGTTAAAACCGCCGAGACCGGACTGCTTAAAAGCATTTGTTCAAATTCGAGCAATTTTTGCATTTGCAGATGTAtc       >  2:4312776/1‑140 (MQ=255)

                                                                                                                                         aGCGACCGCTTCCAGAAAGCAGCCAAAATAAAACCGAGAAAAACCATCATGAAAGCTATTGTTAAAACCGCCGAGACCGGACTGCTTAAAAGCATTTGTTCAAATTCGAGCACTTTTTGCATTTGCAGATGGATcttctt  <  2:4862788/140‑1 (MQ=255)

CAAGCCGATCCCATTTATCAACATCAGCCCAAAAATCGTATTACCTAACGGTGCCCAGCCATTCTCACCTGTGAAACATAAGGACAGGACCACCTTCCCAACAAGTGTCACATTTAATAGGAATAAACCAAAT‑ACAAGCGACCGCTTCCAGAAAGCAGCCAAAATAAAACCGAGAAAAACCATCATGAAAGCTATTGTTAAAACCGCCGAGACCGGACTGCTTAAAAGCATTTGTTCAAATTCGAGCACTTTTTGCATTTGCAGATGGATCTTCTT  >  NZ_CP034943/2897713‑2897988

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`