BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	2,991,252	C→A	22.5%	intergenic (+53/‑154)	gbsR → / → bslA	transcriptional regulator GbsR/biofilm‑surface layer protein BslA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	2,991,252	0	C	A	22.5%	258.7 / 23.4	145	intergenic (+53/‑154)	gbsR/bslA	transcriptional regulator GbsR/biofilm‑surface layer protein BslA
Reads supporting (aligned to +/- strand): ref base C (32/72); new base A (34/3); total (70/75)
Fisher's exact test for biased strand distribution p-value = 5.31e-11
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 149 total aligned reads displayed.

AGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCG‑TTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAAT‑ACGGGAAAAACAT‑CCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCGGGCAGGCCTGAATATTTTTATAACTTT  >  NZ_CP034943/2991115‑2991388

    tACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCATAGCGGGGACATTTTTAAAGTTATACCGATTGATACAAAGTAAAACAAAAACGCCTGGGAACCCGGGCG‑TGATTTTGGTATTTGTTATTGAATtacgagcaa                                                                                                                                      >  2:3560676/1‑134 (MQ=255)

    tACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCG‑TTTTTTTTTTGTTTTTTTTTTTATTAATAaaaa                                                                                                                                      >  1:1467193/1‑140 (MQ=255)

    tACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCG‑TTTTTTTTTTGTTTTTTTTTTTATTAATACaaa                                                                                                                                      >  1:2494291/1‑140 (MQ=255)

    tACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCG‑TGTTTTTTTTTTTTTTTATGTTATTAATAaaaa                                                                                                                                      >  1:1116822/1‑140 (MQ=255)

    tACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCG‑TGTTTTTTTTTGTTTTTTTTTTATTTCTAaaaa                                                                                                                                      >  1:4283248/1‑140 (MQ=255)

    tACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCG‑TGTTTTTGTTTTTTTTTTTGTAATTAATACaaa                                                                                                                                      >  1:713163/1‑140 (MQ=255)

    tACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCG‑TGTTTTTGTTGTTTTTTATGTAATTAAAACaaa                                                                                                                                      >  1:1116903/1‑140 (MQ=255)

    tACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCG‑TGTTTTTGTTCTTTTTTATTTAAAtactaaaca                                                                                                                                      >  2:1684495/1‑136 (MQ=255)

     aCTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGACACCGGGCG‑TGTTTGTGGTAGTTTTTATGTAATTACTACaaaa                                                                                                                                     >  2:4293111/1‑139 (MQ=255)

     aCTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCG‑TTTTTTTGTTATTTTTTATTTAATTACAACaaaa                                                                                                                                     >  2:647241/1‑139 (MQ=255)

     aCTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCG‑TGTTTTTTGTGTTTTTTATGTAATtactaacaat                                                                                                                                     >  2:4281784/1‑135 (MQ=255)

     aCTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCG‑TGTTTTTGTTGTTTTTTATTTGATTACTAAAAAt                                                                                                                                     >  1:2928911/1‑140 (MQ=255)

            ttGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCG‑TGTTTTTTTTGTTTTTTTTTTAATTAATAAAAAATTTaaaa                                                                                                                              >  1:4783487/1‑140 (MQ=255)

               gCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCG‑TTTTTTTTTTCTTTTTTTTGTAATTTATAAAAATTTTAAAAAT‑a                                                                                                                          >  2:2084219/1‑140 (MQ=255)

                cTGAAACGGCTTGCTGACTCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGTGAAACCGGGCG‑TTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAAT‑Ac                                                                                                                         <  2:2235996/140‑1 (MQ=255)

                cTGAAACGGCTGGCTGACTCGGTGCAGAGCGGGGAATTTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCG‑TTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAAT‑Ac                                                                                                                         <  2:4557559/140‑1 (MQ=255)

                   aaaCGGCTTTCTCAATCGGTGCAGAGCGGGGACATTTTTAAATTTAAACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCG‑TGTTTTTGTTAATTTGTATTTAATAAATTAAAATATTAAAAAT‑ACggg                                                                                                                      >  2:2608564/1‑140 (MQ=255)

                   aaaCGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCG‑TTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAAT‑ACggg                                                                                                                      <  2:3203150/140‑1 (MQ=255)

                        gCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCG‑TGTTTTTGTTTTTTTTTTTTTAGTTATTACAAATTTTAAAAAT‑AAGGGaaaaa                                                                                                                 >  1:1815009/1‑140 (MQ=255)

                        gCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCG‑TGTGTTTGTTGTTTTTTTTTTTATTTATACAAAGTTGTAAAATAACGGGaaaa                                                                                                                  >  1:4160405/1‑140 (MQ=255)

                        gCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCG‑TGTGTTTGTTGGTTTTTTTGTTATTAAGACAAAATTGGAAAAT‑ACGGGacaac                                                                                                                 >  1:4592720/1‑139 (MQ=255)

                         gttGCTGAATCGGTCCAGAGCGGGTAAATTTTTAATTTTATACCGATTCAAACAAAGAAAAACAAAAACGCCTGGGAAACCGGGCG‑TTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAAT‑ACGGGAAAAAc                                                                                                                <  1:3938544/139‑1 (MQ=255)

                         cTTGCTTAATCGGTGCAGAGCGGGTAAATTTTTAAATTTATACCGATTCAAACAAAGTAAAACAAACACGCCTGGGAAACCGGGCG‑TTTTTTTGTTCTTTTTTATGTAAGTACTACAAATTTGAAAAAT‑ACGGGAAAAAc                                                                                                                <  1:3175694/140‑1 (MQ=255)

                         cTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAACCTAAAACAAAAACGCCTGGGAAACCGGGCG‑TTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAAT‑ACGGGAAAAAc                                                                                                                <  1:4770431/140‑1 (MQ=255)

                         cTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCG‑TGTTTTTGTTGTTTTTTTTGTAATTAATAAAAAATTGAAAAAT‑AAGGGaaaaaa                                                                                                                >  2:4133324/1‑139 (MQ=255)

                         cTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCG‑TGTTTTTGTTGTTTTTTATGTAATTTATACAAATTTGTAAAAA‑AAGGGaaaaaa                                                                                                                >  2:2223822/1‑139 (MQ=255)

                          ttGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCG‑TGGTTTTGTTTTTTTTTTTTTAATTAAAAAAAATTTTAAAAAA‑AAAGGaaaaaaa                                                                                                               >  1:159481/1‑138 (MQ=255)

                                       gCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTTTTTATTTTTTATTTAATTAATACAAAATTTAAAAAT‑AAAGGTAAAACAA‑ACCAATTaaat                                                                                                  >  2:2533517/1‑137 (MQ=255)

                                          gagGGGGGAAATTTTTAAATTTATACCGATTGAAACAAATTAAAACAAAAACGCCTGGGAAACCGGGCG‑TTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAAT‑ACGGGAAAAACAT‑CCCATTTACTTCCaa                                                                                              <  1:3799419/140‑1 (MQ=255)

                                             cGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCG‑TGTTTTTGGTGTGTTTTAGTTAAATTCTACAAATTATAAAAAA‑ACCGGAAAAACAA‑CACAGTTACTTCAaaaaa                                                                                           >  2:1855262/1‑139 (MQ=255)

                                                ggAAATTTTTAAATTTATACCGATTGAATCAAAGTAAAACAAAAACGCCTGGGACACCGGGCG‑TGTTGGTGTTATTTTTTGTGTGATTACGACAAATTTTTAAAAT‑AAGGGAAAAACAT‑AGGATTTGCTTACAATAGAtt                                                                                        >  2:1075889/1‑140 (MQ=255)

                                                ggAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCG‑TTTTTTTGGTCTTTTTTATGTAATTACTACAAATTTTAAAAAT‑ACGGGAAAAACAA‑GCCAGTTAATTACAAAAAAtt                                                                                        >  2:1243388/1‑140 (MQ=255)

                                                ggAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCG‑TTTTTGTGTTCTTTTTTTTGTAAATAATACAAAATTTTAAAAT‑ACGGGCCAAACAT‑CACAGTTACTTACAAAatagt                                                                                        >  2:4705987/1‑138 (MQ=255)

                                                ggAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCG‑TGTTTTTTTTGTTTTTTTTTTTATTTGTTAAAAATTTTAAAAT‑AAGGGTAAAAACT‑ACCATTTTATTTCAAAATAtt                                                                                        >  1:2336010/1‑140 (MQ=255)

                                                ggAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCG‑TGTTTTTGTTTTTTTTTTTTTTATTTATAAAAAATTTTAAAAA‑AAGGGTAAAAAAA‑ACCAGTTAATTACaaaaaagt                                                                                        >  1:2193709/1‑136 (MQ=255)

                                                ggAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCG‑TGTTTTTGTTGTTTTTTTTGTTAATAGTACAAAATTTTAAAAA‑AAGGGCAAAACAT‑GCCAATTAAGTTCAAAAAAtt                                                                                        >  1:111671/1‑140 (MQ=255)

                                                ggAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCG‑TGTTTTTGTTGTTTTTTATGTAAATACTACAAATTTGAAAAAT‑AACGGAAAAACAG‑AGCAATTAGTTCCAAAaaatg                                                                                        >  2:1519395/1‑139 (MQ=255)

                                                ggAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCG‑TGTTTTTGTTCTTTTTTATGTAATTACTAAAAATTTGTAAAAT‑ACGGGGAAAACAT‑ACCATTTACTTCCAAAATAtt                                                                                        >  2:1022795/1‑140 (MQ=255)

                                                ggAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCG‑TGTTTTTGTTATTTTTTTTGTAATTAATACAAATTTGAAAAAA‑AAGGGAAAAACAT‑ACCAATTTCGTCCCAAATAtt                                                                                        >  2:4818131/1‑140 (MQ=255)

                                                ggAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCG‑TGTTTTTGGTGTTTTTTTTTTTATTTCTACAAATTTTTAAAAT‑AAGGGAAAAAAAT‑GCCCTTTTATTGGAAAATAtt                                                                                        >  1:1732647/1‑140 (MQ=255)

                                                 taattttttAAATTTTTACCTATTTAAACAAAGTAAAAAAAAAACGACTGGGAAACCGGGCT‑TTTTTTTGTTCTTTTTTATGTAATTACGACAAATTTGAAAAAT‑AAGGGAAAAACAT‑CCCATTTACTTCCAAAATAttt                                                                                       <  2:129922/136‑1 (MQ=255)

                                                 gAATTTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCG‑TTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAAT‑ACGGGAAAAACAT‑CCCATTTACTTCCAAAATAttt                                                                                       <  2:1554557/140‑1 (MQ=255)

                                                   aaTTTTTAAATTTATACCTATTTAAAAAAATTAAAACAAAAACGCCTGTGAAACCGGGAG‑ATTTTTTTTTCTTTTTTATGTAATTACTACAAATTTGAAAAAT‑ACGGGAAAAACAT‑CCCATTTACTTCCAAAATATTTGa                                                                                     <  2:1611144/140‑1 (MQ=255)

                                                   aaTTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCG‑TTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAAT‑ACGGGAAAAACAT‑CCCATTTACTTCCAAAATATTTGa                                                                                     <  2:4424276/140‑1 (MQ=255)

                                                   aaTTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCG‑TTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAAT‑ACGGGAAAAACAT‑CCCATTTACTTCCAAAATATTTGa                                                                                     <  2:3997805/140‑1 (MQ=255)

                                                    aTTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCG‑TTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAAT‑ACGGGAAAAACAT‑CCCATTTACTTCCAAAATATTTGAt                                                                                    <  2:3017464/140‑1 (MQ=255)

                                                     tttttAAATTTATACCTATGCAAACAAAGTCAAAAAAAAACGCCTGGGAAACCGGGCG‑TTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAAT‑ACGGGAAAAACAT‑CCCATTGACTTCCAAAATATTTGATg                                                                                   <  1:1335668/140‑1 (MQ=255)

                                                      ttttAAATTTATCCCTATTGAAACAAAGTACAACAAAAACGCCTGGGAAACCGGGCG‑TTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAAT‑ACGGGAAAAACAT‑CCCATTTACTTCCAAAATATTTGATgg                                                                                  <  2:4199654/140‑1 (MQ=255)

                                                      ttttAAATTTATACCTATTGAAAAAAATTAAAACAAACACGCCTGGGAAACCGGGCG‑TTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAAT‑ACGGGAAAAACAT‑CCCATTTACTTCCAAAATATTTGATgg                                                                                  <  1:138617/140‑1 (MQ=255)

                                                       tttAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCG‑TGTTTTTTTTGTTTTTTATGTAATTACTACAAAATTTAAAAAT‑AAGGGGAAAAAAT‑CCCAGTTAATTCCAAAAAATTTTTTGGt                                                                                 >  2:4446289/1‑140 (MQ=255)

                                                                ataCCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCG‑TTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAAT‑ACGGGAAAAACAT‑CCCATTTACTTCCAAAATATTTGATGGTAATTTTATg                                                                        <  2:4032121/140‑1 (MQ=255)

                                                                ataCCGATTGAAACAAACTAAAAAAAAAACGCCTGGGAAACCGGGCG‑TTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAAT‑ACGGGAAAAACAT‑CCCATTTACTTCCAAAATATTTGATGGTAATTTTATg                                                                        <  2:1890746/140‑1 (MQ=255)

                                                                 taCCGATTCAAACACAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAAT‑ACGGGAAAAACAT‑CCCATTTACTTCCAAAATATTTGATGGTAATTTTATg                                                                        <  1:2533517/140‑1 (MQ=255)

                                                                     gATTTAAACAATTTAAAAAAAAACCGCCTGTGAAACCGTTCG‑TTTTTTTGTTCTTTTTTATGTAATTAAGACAAATATGCAAAAT‑ACGGGAAAAACAT‑CCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGt                                                                   <  1:2402917/140‑1 (MQ=255)

                                                                     gATTTAAACAAAGTAAAAAAAAAACGCCTGGGAAACCGGGCG‑TTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAAT‑ACGGGAAAAACAT‑CCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGt                                                                   <  2:950538/140‑1 (MQ=255)

                                                                     gATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCG‑TTGTTTTTTTGTTTTTTATGTTAATACTACAAATTTGAAAAAA‑ACGGGAAAAAAAA‑ACCATTTAATTAAAAAATATTTTATTGTAAATTTTTGTATGt                                                                   >  2:2510388/1‑140 (MQ=255)

                                                                     caTTTAAACAAAGTACACCAAACCCGCCTGGGAAACCGGGCG‑TTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAAT‑ACGGGAAAAACAT‑CCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGt                                                                   <  1:1537752/139‑1 (MQ=255)

                                                                      aTTGAAACAAAGTAAAACAAAAACTCCTGGGAAACCGGGCG‑TTTTTTTTTTATTTTTTATGTAATAAATAAAAAAATTAAAAAA‑AAAGGAAAAAAATAACAATTTAATACAAAAAAATTTTAT‑ATAATTTTATTTAGTTa                                                                  >  2:3742891/1‑140 (MQ=255)

                                                                      aTTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCG‑TTTTTTTGTTGGTTTTTATTTAATTAATACAAAATTGAAAAAT‑ACGGGAAAAAAAT‑CCCCTTTACTTGCAAAATATTTGATGGTTATTTTATGTAGGTa                                                                  >  2:3503970/1‑140 (MQ=255)

                                                                          aaaaaaAGTAAAACAAACACGCCGGGTAAACCGGGCT‑TTTTTTTGTTCTTTTTTATGTAATTACTACAACTTTGAAAAAT‑ACGGGAAAAACAT‑CCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAAt                                                              <  2:2748019/140‑1 (MQ=255)

                                                                          aaaCACAGTACAACAAACACGCCTGGGAAACCGGGCG‑TTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAAT‑ACGGGAAAAACAT‑CCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAAt                                                              <  2:3299327/140‑1 (MQ=255)

                                                                          aaaCAAAGTAAAACAAACACGCCTGGGAAACCGGGCG‑TTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAAT‑ACGGGAAAAACAT‑CCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAAt                                                              <  1:4133324/140‑1 (MQ=255)

                                                                          aaaCAAAGTAAAACAAAAACGCCTGGGAAACCGGGCG‑TTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAAT‑ACGGGAAAAACAT‑CCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAAt                                                              <  2:2628707/140‑1 (MQ=255)

                                                                               aaGTAAAACAAAAACGCCTGGGAAACCGGGCG‑TTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAAT‑ACGGGAAAAACAT‑CCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTa                                                         <  1:2097745/140‑1 (MQ=255)

                                                                               aaGTAAAACAAAAACGCCTGGGAAACCGGGCG‑TTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAAT‑ACGGGAAAAACAT‑CCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTa                                                         <  1:4660839/140‑1 (MQ=255)

                                                                                    aaacaaacACGCCTGGGAAACCGGGCG‑TTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAAT‑ACGGGAAAAACAT‑CCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                                                    <  1:2372401/140‑1 (MQ=255)

                                                                                    aaacaaacACGCCTGGGAAACCGGGCG‑TTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAAT‑ACGGGAAAAACAT‑CCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                                                    <  1:4917180/140‑1 (MQ=255)

                                                                                    aaacaaacACGCCTGGGAAACCGGGCG‑TTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAAT‑ACGGGAAAAACAT‑CCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                                                    <  1:4852113/140‑1 (MQ=255)

                                                                                    aaacaaacACGCCTGGGAAACCGGGCG‑TTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAAT‑ACGGGAAAAACAT‑CCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                                                    <  1:764544/140‑1 (MQ=255)

                                                                                    aaacaaacACGCCTGGGAAACCGGGCG‑TTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAAT‑ACGGGAAAAACAT‑CCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                                                    <  1:2652865/140‑1 (MQ=255)

                                                                                    aaaaaaACACGCCTGGGAAACCGGGCG‑TTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAAT‑ACGGGAAAAACAT‑CCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                                                    <  1:4806641/140‑1 (MQ=255)

                                                                                    aaaCAAAATCTCCTGGGAAACCGTTAG‑TTTTTTTGTTATTTTTTATGTAATTAATACATATTTTAAAATT‑ACGGGAAACACAT‑CCCATTTACTTCCAAAATATTTGATGTTTTTTTTATGTATTTAAAATTTGTtttttg                                                    <  2:2184387/140‑3 (MQ=255)

                                                                                    aaaCAAAAACGCCTGGGAAACCGGGCG‑TTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAAT‑ACGGGAAAAACAT‑CCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                                                    <  1:646570/140‑1 (MQ=255)

                                                                                    aaaCAAAAACGCCTGGGAAACCGGGCG‑TTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAAT‑ACGGGAAAAACAT‑CCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                                                    <  1:5068181/140‑1 (MQ=255)

                                                                                    aaaCAAAAACGCCTGGGAAACCGGGCG‑TTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAAT‑ACGGGAAAAACAT‑CCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                                                    <  1:980522/140‑1 (MQ=255)

                                                                                    aaaCAAAAACGCCTGGGAAACCGGGCG‑TTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAAT‑ACGGGAAAAACAT‑CCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                                                    <  2:2805371/140‑1 (MQ=255)

                                                                                     aaCAAAAACGCCTGGGAAACCGGGCG‑TTTTTTTGTTCTTTTTTTTGTAAATAAAAAAAATATTAAAAAT‑AAGGGAAAAAAAA‑CCCAATTAAATGAAAAAAAATTTTTTGTAATTTTAATTAGGGAAAATTTATAtttatc                                                   >  2:2262241/1‑137 (MQ=255)

                                                                                     aaCAAAAACGCCTGGGAAACCGGGCG‑TGTTTTTTTTGTTTTTTATTTAATTAAAAAAAATTTGAAAAAA‑ACGGGAAAAAAAT‑GCCATTTTAATCCCAAATATTTGTTGGTAATTTTTTGTAGTTTAAATTTGTAtttttg                                                   >  2:4315314/1‑137 (MQ=255)

                                                                                     aaCAAAAACGCCTGGGAAACCGGGCG‑TGGTTTTGTTGTTTTTTTTTTAAATACTAAAAAATTTAAAAAT‑AAGGGAAAAAAAT‑CCCATTTACTTCCAAAATAATTGATTGTAATTTTATGTAGGTAAAATTTTTATTTTGc                                                   >  2:4965216/1‑140 (MQ=255)

                                                                                         aaaaCGCCTGGGAAACCGGGCG‑TTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAAT‑ACGGGAAAAACAT‑CCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGtt                                               <  1:3779373/140‑1 (MQ=255)

                                                                                         aaaaCGCCTGGGAAACCGGGCG‑TTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAAT‑ACGGGAAAAACAT‑CCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGtt                                               <  1:3906075/140‑1 (MQ=255)

                                                                                              gCCTGGGAAACCGGGCG‑TTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAAT‑ACGGGAAAAACAT‑CCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCagag                                          <  1:2223822/140‑1 (MQ=255)

                                                                                              gCCTGGGAAACCGGGCG‑TTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAAT‑ACGGGAAAAACAT‑CCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCagag                                          <  1:815739/140‑1 (MQ=255)

                                                                                              gCCTGGGAAACCGGGCG‑TTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAAT‑ACGGGAAAAACAT‑CCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCagag                                          <  1:4474903/140‑1 (MQ=255)

                                                                                              gCCTGGGAAACCGGGCG‑TTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAAT‑ACGGGAAAAACAT‑CCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCagag                                          <  1:2262241/140‑1 (MQ=255)

                                                                                                        ccGGGCG‑TTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAAT‑ACGGGAAAAACAT‑CCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGaaaa                                <  2:925173/140‑1 (MQ=255)

                                                                                                             cG‑TTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAAT‑ACGGGAAAAACAT‑CCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCggg                           <  1:4198281/140‑1 (MQ=255)

                                                                                                             cG‑TTTTTTTGTTCTTTTTTATGTAATTACGACAAATTTGAAAAAT‑ACGGGAAAAACAT‑CCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCggg                           <  2:498031/140‑1 (MQ=255)

                                                                                                                   ttttGTTCATTTTTATGTAATGACTACAAATTTGAAAAAT‑ACGGGAAAAACAT‑CCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCGGGCAGGc                      <  1:2635101/140‑1 (MQ=255)

                                                                                                                     ttGTTCTTTTTTATGTAATTACTACAAATTTGAAAAAT‑ACGGGAAAAACAT‑CCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCGGGCAGGCCt                    <  2:3913255/140‑1 (MQ=255)

                                                                                                                       gtacTTTTTTATGTAATTACTACAAATTTGAAAAAT‑ACGGGAAAAACAT‑CCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCGGGCCGGCCTGa                  >  2:4108285/4‑140 (MQ=255)

                                                                                                                        ttCTTTTTTATGTAATTACTACAAATTTTAAAAAT‑ACGGGAAAAACAT‑CCCATTTACTTCCAAAATATTTTATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCGGGCAGGCCTGaa                 >  2:3834186/1‑140 (MQ=255)

                                                                                                                         tCTTTTTTATGTAATTACTACAAATTTGAAAAAT‑ACGGGAAAAACAT‑CCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCGGGCAGGCCTGAat                <  2:3774704/140‑1 (MQ=255)

                                                                                                                             ttttATGTAATTACTACAAATTTGAAAAAT‑ACGGGAAAAACAT‑CCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCGGGCAGGCCTGAATAttt            >  2:3161992/1‑140 (MQ=255)

                                                                                                                             ttttATGTAATTACTACAAATTTGAAAAAT‑ACGGGAAAAACAT‑CCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCGGGCAGGCCTGAATAttt            >  2:2944861/1‑140 (MQ=255)

                                                                                                                                  tGTAATTACTACAAATTTGAAAAAT‑ACGGGAAAAACAT‑CCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCGGGCAGGCCTGAATATTTTtata       <  1:736669/140‑1 (MQ=255)

                                                                                                                                       ttACTACAAATTTGAAAAAT‑ACGGGAAAAACAT‑CCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCGGGCAGGCCTGAATATTTTTATAACttt  >  2:4610973/1‑140 (MQ=255)

                                                                                                                                       ttACTACAAATTTGAAAAAT‑ACGGGAAAAACAT‑CCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCGGGCAGGCCTGAATATTTTTATAACttt  >  2:3246262/1‑140 (MQ=255)

                                                                                                                                       ttACTACAAATTTGAAAAAT‑ACGGGAAAAACAT‑CCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCGGGCAGGCCTGAATATTTTTATAACttt  >  2:512512/1‑140 (MQ=255)

                                                                                                                                       ttACTACAAATTTGAAAAAT‑ACGGGAAAAACAT‑CCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCGGGCAGGCCTGAATATTTTTATAACttt  >  2:2328568/1‑140 (MQ=255)

AGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCG‑TTTTTTTGTTCTTTTTTATGTAATTACTACAAATTTGAAAAAT‑ACGGGAAAAACAT‑CCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCGGGCAGGCCTGAATATTTTTATAACTTT  >  NZ_CP034943/2991115‑2991388

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`