BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	3,746,916	G→T	6.4%	T51K (ACA→AAA)	EO946_RS19275 ←	galactokinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	3,746,916	0	G	T	6.4%	771.2 / 7.3	302	T51K (ACA→AAA)	EO946_RS19275	galactokinase
Reads supporting (aligned to +/- strand): ref base G (87/183); new base T (29/3); total (116/186)
Fisher's exact test for biased strand distribution p-value = 1.27e-10
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 313 total aligned reads displayed.

GCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGT‑ACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATCCCCTTTAG  >  NZ_CP034943/3746777‑3747055

gCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATg                                                                                                                                              <  1:1497233/140‑1 (MQ=255)

            gTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTGTGCAACAGCCGCCTATTT‑ACCCAATGTCa                                                                                                                                 >  1:3816133/1‑140 (MQ=255)

            gTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTGTGGTTCTGGAACCGCAACAAATTT‑AACCAATGtgc                                                                                                                                 >  1:2023451/1‑138 (MQ=255)

            gTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTGTCTTTCTGCAACACCAGCATATTT‑ACCCATTGTCa                                                                                                                                 >  2:3112403/1‑140 (MQ=255)

             ttCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTGTGTTTGTGCAACAGCACCAAATTT‑ACCCATTGTAAg                                                                                                                                >  1:2875504/1‑140 (MQ=255)

               cGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGT‑ACCCATTGTCAgcg                                                                                                                              <  1:308837/140‑1 (MQ=255)

               cGCCCAGTAGTCTTCTTTTTGATAGAGGAGGTCGTCTAGGATGCAATCATTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGAAAACCCCGTCTTTTCTTTGAGCAAAACCAGCCAATTT‑ACCCAAAGTCatcc                                                                                                                              >  2:1380352/1‑137 (MQ=255)

                 cccAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTGTTTGTTCAAAAGCAGCAAAAGT‑ACCCCATGTAAgcgcc                                                                                                                            >  1:354506/1‑139 (MQ=255)

                 cccAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTTCAACAACAGCAAATGT‑AACCAATGTCaacccg                                                                                                                            >  1:4924674/1‑135 (MQ=255)

                 cccAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGT‑ACCCATTGTCAgcgcg                                                                                                                            <  1:1810414/140‑1 (MQ=255)

                 cccAGTCGTCTTCTTTTCGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTACAGTTGTCTGACTCCATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGT‑ACCCATTGTCAgcgcg                                                                                                                            <  1:2287592/140‑1 (MQ=255)

                   cAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGT‑ACCCCTTGTCAGCGCGCa                                                                                                                          <  1:3674288/140‑1 (MQ=255)

                     gtcgtcTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGACTACATCCGGACAACCCCGTCGTGTCTTTGTGAAACACCCGCATATTT‑AACCAATGTAAGCGCGCACg                                                                                                                        >  2:4533360/1‑140 (MQ=255)

                      tcgtcTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCATTAAGCAACACCACCAAATGT‑ACCCATTGTCACCGCGCaacg                                                                                                                       >  2:4717173/1‑137 (MQ=255)

                       cgtcTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGT‑ACCCATTGTCAGCGCGCACGGa                                                                                                                      <  2:192989/140‑1 (MQ=255)

                         tcttctTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATAAATCCGGACAACCCCGTCGTTTTTTTGTGGAAAATCAACAAAATT‑AACCCTTTTCAGCGCGCAACGaaa                                                                                                                    >  1:266456/1‑140 (MQ=255)

                                tttGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTGTCTTTGTGCAAAAGCACCAAATGTAACCCATT‑TCACCGCGCACGGAAAcacaatc                                                                                                             >  1:2309227/1‑137 (MQ=255)

                                 ttGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGT‑ACCCATTGTCAGCGCGCACGGAAACACATGcc                                                                                                            <  1:3131460/140‑1 (MQ=255)

                                 ttGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGT‑ACCCATTGTCAGCGCGCACGGAAACACATGcc                                                                                                            <  1:4415639/140‑1 (MQ=255)

                                 ttGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGT‑ACCCATTGTCAGCGCGCACGGAAACACATGcc                                                                                                            <  1:733793/140‑1 (MQ=255)

                                 ttGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGT‑ACCCATTGTCAGCGCGCACGGAAACACATGcc                                                                                                            <  1:2446621/140‑1 (MQ=255)

                                 ttGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGT‑ACCCATTGTCAGCGCGCACGGAAACACATGcc                                                                                                            <  2:1085337/140‑1 (MQ=255)

                                 ttGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTGTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGT‑ACCCATTGTCAGCGCGCACGGAAACACATGcc                                                                                                            <  2:2190260/140‑1 (MQ=255)

                                 ttGATAGCGGATGTCGTCTAGGCTGCAGTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGT‑ACCCATTGTCAGCGCGCACGGAAACACATGcc                                                                                                            <  1:235475/140‑1 (MQ=255)

                                   gagaGCGGATGTCGTCTAGGCTGCATTCCTTGCGCCCGCCCTCTGTACAGTTGTCGGAATACCTCCGGACAACCCCGGCGTGTCTTTCTGCAACAGCAGCATATGT‑ACCCCTTGTCAGCGCGCACGGAAACACATGCccg                                                                                                          <  2:256545/137‑1 (MQ=255)

                                   gATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGT‑ACCCATTGTCAGCGCGCACGGAAACACATGCccg                                                                                                          <  2:3627782/140‑1 (MQ=255)

                                   gATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGT‑ACCCATTGTCAGCGCGCACGGAAACACATGCccg                                                                                                          <  2:4233450/140‑1 (MQ=255)

                                   gATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGT‑ACCCATTGTCAGCGCGCACGGAAACACATGCccg                                                                                                          <  2:692455/140‑1 (MQ=255)

                                   gATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGT‑ACCCATTGTCAGCGCGCACGGAAACACATGCccg                                                                                                          <  2:4821470/140‑1 (MQ=255)

                                   gATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGT‑ACCCATTGTCAGCGCGCACGGAAACACATGCccg                                                                                                          <  2:1487590/140‑1 (MQ=255)

                                   gATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGGCGTGTCTTTCTGCAACAGCAGCATATGT‑ACCCCTTGTCAGCGCGCACGGAAACACATGCccg                                                                                                          <  1:873014/140‑1 (MQ=255)

                                   gATAGCGGATGTCGTCTAGGCTGCATTCCGTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGT‑ACCCATTGTCAGCGCGCACGGAAACACATGCccg                                                                                                          <  1:4643949/140‑1 (MQ=255)

                                   gATAGCGGATGTCGTCGAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGT‑ACCCATTGTCAGCGCGCACGGAAACACATGCccg                                                                                                          <  2:1563098/140‑1 (MQ=255)

                                   gATAGCGGAGGTCGTCTAGGCTGCATTCCTGGATCCCTCCGTCTTTAAAGTGGTCTGACTACATCCGGACAACCCCGTCGTTTCTTTCTGCAACACCAGCATATGT‑ACCCATTGTCAGCGCGCACGGAAACACATGCccg                                                                                                          <  1:3736874/140‑1 (MQ=255)

                                        cGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGT‑ACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGtt                                                                                                     <  1:4533360/140‑1 (MQ=255)

                                        cGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGT‑ACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGtt                                                                                                     <  2:4754510/140‑1 (MQ=255)

                                          gATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGT‑ACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTtat                                                                                                   <  2:588266/140‑1 (MQ=255)

                                          gATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTGTCTTTCTGCAACAGCAGCATATGT‑ACCCATTGTCAGCGCGCACGGAAACACAAGCCCGCCGTtat                                                                                                   >  2:4138487/1‑140 (MQ=255)

                                          gATGTCGTCTAGGCTGCAGTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGT‑ACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTtat                                                                                                   <  1:2570072/140‑1 (MQ=255)

                                           aTGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGT‑ACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTtata                                                                                                  <  2:2709058/140‑1 (MQ=255)

                                              tcgtcTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGT‑ACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATc                                                                                               <  1:501481/140‑1 (MQ=255)

                                                 tcTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTGTTTCTGCAACAGCAGCATATGT‑ACCCCTTTTCAGCGCGCCCGGAAAAACAAGCCCGCCGTTATAATCTGt                                                                                            >  2:2029352/1‑140 (MQ=255)

                                                 tcTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGGACCAGCAGCATATGTAACCCATT‑TCAGCGCGCACCGAAACACATGCCCGCCGTTATAATATGt                                                                                            >  2:265216/1‑140 (MQ=255)

                                                   tAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGTCAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGT‑ACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTAt                                                                                          <  1:1191329/140‑1 (MQ=255)

                                                   tAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGT‑ACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTAt                                                                                          <  1:661396/140‑1 (MQ=255)

                                                   tAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGT‑ACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTAt                                                                                          <  1:1424119/140‑1 (MQ=255)

                                                      gCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACGCCGTCGTTTCTTTCTGCAACAGCAGCATATGT‑ACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGtt                                                                                       <  1:2519943/140‑1 (MQ=255)

                                                        tGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTGTTTAATCAACCGCACCAAATGT‑ACCCATTGTCACCGCGCACCGAAACACAAGCCCGCCGTTATAAACTGTAttttat                                                                                     >  2:1123354/1‑138 (MQ=255)

                                                        tGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCGTTGTGCAACACCAGCATATGT‑AACCAATGTCAGCGCGCACGGAAACACAAGCCCGCCGTTATAATATGTATGTtct                                                                                     >  2:828495/1‑140 (MQ=255)

                                                          cATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTGTTTCTTCAACAGAAGCATATGT‑ACCCAATGTGAGCGCGCACGGAAACAAATGCACGCCCTTAAAATATGTATTTTATcc                                                                                   >  2:4163271/1‑140 (MQ=255)

                                                          cATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATTT‑AACCATTGTGAGCGCGCACGGAAACACATGACCGCCGTTATAATCTGTATGTTCTcc                                                                                   >  2:1440919/1‑140 (MQ=255)

                                                          cATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTGTCTTTCTTCAACAGCAGCATATGT‑ACACAATGTCAGCGCCCACGGAAAAAAAAGCCCGCCCGTATAAAATGTATGTtctac                                                                                   >  2:3216973/1‑138 (MQ=255)

                                                          cATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTGTCTTTCTGCAACAGCAGCATATGT‑AACCAATGTCAGCGCGCACGGAAACACAAGACCGCCCTTATAATCTGTATGTTATcc                                                                                   >  2:3566330/1‑140 (MQ=255)

                                                          cATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTGTCTTTAGGCACCAGCAGCCTATGT‑AACCAATGTCAGCGCCCACGGGAAAAACTGCCCGCCGTTATAAAATGTATGTTGTcc                                                                                   >  1:1966518/1‑140 (MQ=255)

                                                             tCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGT‑ACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAAt                                                                                <  2:4115349/140‑1 (MQ=255)

                                                             tCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGT‑ACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAAt                                                                                <  1:4803903/140‑1 (MQ=255)

                                                                    tCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATAAGT‑ACCCATTGTAAGCGCGCACGGAAACACATGCCCGCCGTTATAATATGTATGTTCTCCAATCAGAtta                                                                         >  2:2336057/1‑139 (MQ=255)

                                                                          cGTCTTTAAAGTTGTCTGAATACATCCGGACCACCCCGTCGTGTGGTTGTGCAACACCAGCATATTT‑ACCCCTGGTCAGCCTGCACCGAAACACAGGCCCGCCGTTATAATGGGTGTGTTGTCGCCTCCGATTCAcggtt                                                                   >  1:3278496/1‑136 (MQ=255)

                                                                          cGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTGTTTCTGCAAAACCAGCCAATGT‑AACCAATGTCAGCGCGCACCGAAACAAAATCCCGCCGGTAAAATAAGTATGTTATACAATAAGAtttaaaatt                                                                   >  1:5161893/1‑133 (MQ=255)

                                                                                tAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTGTGTTTGTGCAACAGCAGCATATTT‑ACCCAGTGTCAGCGCGCCCGGAAACAAATGCCCGCCGGTATAATATGTATGTTGTCCCATCAGATTCACTATTACAggg                                                             >  1:5060971/1‑139 (MQ=255)

                                                                                       gTCTGAATACATCCGGACACCCCCGTCGTTTCTTTATGCAACAGCCACATATTT‑ACCCAATGTAAGCGCGCACGGAAACAAAAGCCCGCCCTTATAATCTGTTTTTTATCCAAACACATTAACTCTTCCACGTGCGaaaa                                                      >  2:124871/1‑140 (MQ=255)

                                                                                       gTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGT‑ACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTtat                                                                                                   >  2:2793156/1‑95 (MQ=255)

                                                                                       gTCTGAAAACATCCGGACAACCCCGTCGTTTATTTCTGCAAAAGCATCAAATGT‑AAACATTGTCAGCCCGCACGGAAAAAAAAAGCCGCCGTTATAATAATTAAGTTCTACAATCAGTTTCACACATAAAGGTGAGaaaa                                                      >  2:1048791/1‑140 (MQ=255)

                                                                                        tCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGT‑ACCCATTGTCAGCGCGCACGGCAACACAAGCCCGCCGTTATAATCTGTATGTTCTCCAAAGAGATTCAAACTTACACGTTCGaaaaa                                                     >  2:3920822/1‑140 (MQ=255)

                                                                                        tCTGAATACATCCGGACAACCCCGTCGTGTCTTTCTGCAACAGCAGCATATGT‑ACCCCTTGTCAGCGCGCACGGAAACACCAGCCCGCCGTTATAATATGTATGTTGTCCCATCAGATTCACTATTCCAGGTGCGacaaa                                                     >  2:4009262/1‑140 (MQ=255)

                                                                                          tGAATACATCCGGACAACCCCGTCGTTTCTTTGTGCAACAGCAGCATATGT‑AACCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATGTGTATGTTATCCAATCAGATTCACACTTCCAGGTGCGaaaaaaa                                                   >  2:3339104/1‑140 (MQ=255)

                                                                                               aCATCCGGACAACCCCGTCGTTTGTTTATGCAACAGCAGCATATGT‑ACCCCTGGGCAGCGCGCACGGAAAAACAAGCCCGCCGTTAAAATCTGTATGTTGTCCCAACAGATTAACACTTACAGGGTCGAAAAAAAACCGt                                              >  2:5044/1‑140 (MQ=255)

                                                                                               aCATCCGGACAACCCCGTCGTTTCTTTAAGCAAAACCAGCAAATTT‑AACCATAGACAGCGCGCACCGAAAAACAAGCCCCCCCTTATAATATGTATGTTCTCCAATCAGATTAAAACATCCAGGTGCCaaaaaaaaacca                                              >  2:3734820/1‑136 (MQ=255)

                                                                                                  tCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGT‑ACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAAc                                           <  2:1640027/140‑1 (MQ=255)

                                                                                                  tCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGT‑ACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAAc                                           <  1:1301532/140‑1 (MQ=255)

                                                                                                  tCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGT‑ACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAAc                                           <  1:2775823/140‑1 (MQ=255)

                                                                                                  tCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGT‑ACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAAc                                           <  2:4830859/140‑1 (MQ=255)

                                                                                                       aCAACCCCGTCGTTTGTTTGTGCAACACCACCGTATGT‑TACCCTTGTCAGCGCCCACGGAAACACAAGCCCGCCGTTATAATCTGTATTTTATCCAATCAGATTTAATCTTACAGGTGCGAAAAAAAAACGGAAccccac                                      >  2:4330661/1‑138 (MQ=255)

                                                                                                           ccccGTCGTTTCTTTCTGCAACAACAGCAAATGT‑ACCCATTGTCACCGCGCCCGGAAACACAAGCCCCCCCTTATAAACTGTATTTTCTACAATCAGATTCAAAATTACAGGTGGGTAAAAAAAACGGAACACCTCgttt                                  >  2:1843013/1‑140 (MQ=255)

                                                                                                            cccGTCGTTTCTTTCTGCAACAGCAGCATATGT‑ACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAACCCCTCttttt                                 <  1:2799979/140‑1 (MQ=255)

                                                                                                             ccGTCGTTTCTTTCTGCAACAGCAGCATATGT‑ACCCAGTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCG‑AAAAAAACCGTAACCCCTCTTTTtct                               <  2:2374272/140‑1 (MQ=255)

                                                                                                              cgtcgtTTCTTTCTGCAACAGCAGCATATGT‑ACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAACCCCTCTTTTtct                               <  2:338005/140‑1 (MQ=255)

                                                                                                              cgtcgtTTCTTTCTGCAACAGCAGCATATGT‑ACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAACCCCTCTTTTtct                               <  1:2118173/140‑1 (MQ=255)

                                                                                                              cgtcgtTTCTTTCTGCAACAGCAGCATATGT‑ACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAACCCCTCTTTTtct                               <  2:2301241/140‑1 (MQ=255)

                                                                                                                     tgtttatGCAAAAACAGCATATTT‑ACCCAATGTCAGCGCGCACGGAAACAAAAGCCCCCCCTTATAATATGTAATTTATACAATAAGATTAAAGCATCCCAGTTCGAAAAAAAAACCTAAAACCACATTTTATACGaaaa                        >  2:1737063/3‑138 (MQ=255)

                                                                                                                     tctttctGCAAGAGCAGCATATGT‑ACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAACCCCTCTTTTTCTCCGAATa                        <  2:4427595/140‑1 (MQ=255)

                                                                                                                     tctttctGCAACAGCAGCATATGT‑ACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAACCCCTCTTTTTCTCCGAATa                        <  1:4764482/140‑1 (MQ=255)

                                                                                                                         tctGCAACAGCAGCATATGT‑ACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACt                    <  2:1186078/140‑1 (MQ=255)

                                                                                                                           tGCAACAGCAGCATATGT‑ACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTg                  <  2:86853/140‑1 (MQ=255)

                                                                                                                             cAACAGCAGCATATGT‑ACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCa                >  1:3942684/1‑140 (MQ=255)

                                                                                                                                cagcagCATATGT‑ACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAat             >  1:438965/1‑140 (MQ=255)

                                                                                                                                 agcagcATATGT‑ACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAata            <  1:3566330/140‑1 (MQ=255)

                                                                                                                                  gcagcaTATGT‑ACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAatat           <  1:4142568/140‑1 (MQ=255)

                                                                                                                                   cagcaTATGT‑ACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATc          <  2:2453357/140‑1 (MQ=255)

                                                                                                                                   cagcaTATGT‑ACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTAGGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATc          <  1:4300816/140‑1 (MQ=255)

                                                                                                                                   cagcaTATGT‑ACCCATTGTCAGCGAGCACGGAAACACATTCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATc          <  2:3500652/140‑1 (MQ=255)

                                                                                                                                       atatGT‑ACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATCCCCt      <  1:776996/140‑1 (MQ=255)

                                                                                                                                       atatGT‑ACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATCCCCt      <  1:4500042/140‑1 (MQ=255)

                                                                                                                                       atatGT‑ACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATCCCCt      <  1:1342011/140‑1 (MQ=255)

                                                                                                                                        tatGT‑ACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATCCCCtt     <  2:4376652/140‑1 (MQ=255)

                                                                                                                                        tatGT‑ACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATCCCCtt     >  1:1055445/1‑140 (MQ=255)

                                                                                                                                        tatGT‑ACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATCCCCtt     <  2:390139/140‑1 (MQ=255)

                                                                                                                                         atGT‑ACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATCCCCttt    <  1:1673740/140‑1 (MQ=255)

                                                                                                                                          tGT‑ACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATCCCCTTTa   >  2:4892138/1‑140 (MQ=255)

                                                                                                                                           gT‑ACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATCCCCTTTAg  <  2:2685265/140‑1 (MQ=255)

GCCTTTCGGATAGTTCGCCCAGTCGTCTTCTTTTTGATAGCGGATGTCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGT‑ACCCATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATCCCCTTTAG  >  NZ_CP034943/3746777‑3747055

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`