BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	3,746,962	C→A	8.8%	D36Y (GAT→TAT)	EO946_RS19275 ←	galactokinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	3,746,962	0	C	A	8.8%	646.9 / 9.7	266	D36Y (GAT→TAT)	EO946_RS19275	galactokinase
Reads supporting (aligned to +/- strand): ref base C (89/136); new base A (34/2); total (127/139)
Fisher's exact test for biased strand distribution p-value = 1.24e-10
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 272 total aligned reads displayed.

TCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGT‑ACCC‑‑ATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCG‑AAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATCCCCTTTAGCTCTTCCCGCATGTGCAATCACCTTTCATTGTTTTATTTTTTTATT  >  NZ_CP034943/3746823‑3747101

tcgtcTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGT‑ACCC‑‑ATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATc                                                                                                                                              <  2:1239623/140‑1 (MQ=255)

tcgtcTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGT‑ACCC‑‑ATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATc                                                                                                                                              <  2:4864871/140‑1 (MQ=255)

tcgtcTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGT‑ACCC‑‑ATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATc                                                                                                                                              <  1:501481/140‑1 (MQ=255)

   tcTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTGTTTCTGCAACAGCAGCATATGT‑ACCC‑‑CTTTTCAGCGCGCCCGGAAAAACAAGCCCGCCGTTATAATCTGt                                                                                                                                           >  2:2029352/1‑140 (MQ=255)

   tcTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGGACCAGCAGCATATGTAACCC‑‑ATT‑TCAGCGCGCACCGAAACACATGCCCGCCGTTATAATATGt                                                                                                                                           >  2:265216/1‑140 (MQ=255)

     tAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGTCAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGT‑ACCC‑‑ATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTAt                                                                                                                                         <  1:1191329/140‑1 (MQ=255)

     tAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGT‑ACCC‑‑ATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTAt                                                                                                                                         <  1:985119/140‑1 (MQ=255)

     tAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGT‑ACCC‑‑ATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTAt                                                                                                                                         <  1:770538/140‑1 (MQ=255)

     tAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGG‑ACCC‑‑ATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTAt                                                                                                                                         <  2:3575045/140‑1 (MQ=255)

        gCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACGCCGTCGTTTCTTTCTGCAACAGCAGCATATGT‑ACCC‑‑ATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGtt                                                                                                                                      <  1:2519943/140‑1 (MQ=255)

          tGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTGTTTAATCAACCGCACCAAATGT‑ACCC‑‑ATTGTCACCGCGCACCGAAACACAAGCCCGCCGTTATAAACTGTAttttat                                                                                                                                    >  2:1123354/1‑138 (MQ=255)

            cATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTGTTTCTTCAACAGAAGCATATGT‑ACCC‑‑AATGTGAGCGCGCACGGAAACAAATGCACGCCCTTAAAATATGTATTTTATcc                                                                                                                                  >  2:4163271/1‑140 (MQ=255)

            cATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTGTTTCTGCAACACCAGCATATTT‑AACC‑‑ATTGTCAGCGCGCACGGAAACAAATGCCCCCCGGTATAATATTTAttttatac                                                                                                                                  >  2:4425713/1‑136 (MQ=255)

            cATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTGTTTATGCAACAGCAGCAAAATT‑ACCCCAATTACCAGC‑C‑CACGGAAACACATTACCGCACTTATAATATTTAttttatac                                                                                                                                  >  2:2007108/1‑136 (MQ=255)

            cATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGT‑ACCC‑‑ATTGTCAGCGCGCACGGAAACACAAGCCCGCCGGTATAATAAGTATGTTGTcc                                                                                                                                  >  2:4347442/1‑140 (MQ=255)

            cATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCGTTCTGCAAAAGCAGCATATTT‑ACCC‑‑ATTGTCAGCCCGCACGGAAACACATTCCCCCCGGTATAATATGTATGttatac                                                                                                                                  >  2:2363228/1‑136 (MQ=255)

            cATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTGTTTTTAAGGCACAACCGCCTAAGT‑ACCC‑‑AATATCACCCCGCACGCAAACACAAACCCGCCCGTATAAACTGTAttttgtac                                                                                                                                  >  1:3108219/1‑136 (MQ=255)

               tCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGT‑ACCC‑‑ATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAAt                                                                                                                               <  1:2416839/140‑1 (MQ=255)

               tCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGT‑ACCC‑‑ATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAAt                                                                                                                               <  1:4592637/140‑1 (MQ=255)

               tCCTTGATCCCTACGACTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGT‑ACCC‑‑ATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAAt                                                                                                                               <  1:3790981/140‑1 (MQ=255)

                      tCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTGTTTCTGGAACATCACCATATGT‑AAAC‑‑AATTTCACAGCGCCCCGCAAAACCAGGCCGCCGGAATAATAGGTGTGTTGTCCACTCAgtatc                                                                                                                        >  1:4395397/1‑136 (MQ=255)

                      tCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGT‑ACCC‑‑ATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTc                                                                                                                        <  2:141248/140‑1 (MQ=255)

                      tCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATAAGT‑ACCC‑‑ATTGTAAGCGCGCACGGAAACACATGCCCGCCGTTATAATATGTATGTTCTCCAATCAGAtta                                                                                                                        >  2:2336057/1‑139 (MQ=255)

                      tCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCACCATAAGT‑AACC‑‑ATTGTCAGCGCGCAACGAAACACAATCCCGCCGTTATAATAATTATGTTCTACAAACAGAtta                                                                                                                        >  2:2285824/1‑139 (MQ=255)

                        ccTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGT‑ACCC‑‑ATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCAc                                                                                                                      <  2:1766978/140‑1 (MQ=255)

                           aCGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTGTCTTTCGTCAACACCAGCATATGT‑AACC‑‑ATTGTCAGCGCGCCAGGAAACACATGCCCGCCGTTAAAATATGTATTTTCTCCAATAAGATTccatat                                                                                                                   >  2:4821707/1‑135 (MQ=255)

                            cGTCTTTAAAGTTGTCTGAATACATCCGGACCACCCCGTCGTGTGGTTGTGCAACACCAGCATATTT‑ACCC‑‑CTGGTCAGCCTGCACCGAAACACAGGCCCGCCGTTATAATGGGTGTGTTGTCGCCTCCGATTCAcggtt                                                                                                                  >  1:3278496/1‑136 (MQ=255)

                            cGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTGTTTCTGCAAAACCAGCCAATGT‑AACC‑‑AATGTCAGCGCGCACCGAAACAAAATCCCGCCGGTAAAATAAGTATGTTATACAATAAGAtttaaaatt                                                                                                                  >  1:5161893/1‑133 (MQ=255)

                                         gTCTGAATACATCCGGACAACCCCGTCGTTTCGTTATGCAACACCAGCATATGT‑ACCC‑‑ATGGTCAGCGCGCACGGAAACACAAGCCCCCCGTTATAATCTGTATGTTCTCCAAACAGATTCAATGTTCCAGGTGCG‑aaaa                                                                                                    >  2:244685/1‑140 (MQ=255)

                                         gTCTGAAAACATCCGGACAACCCCGTCGTTTATTTCTGCAAAAGCATCAAATGT‑AAAC‑‑ATTGTCAGCCCGCACGGAAAAAAAAAGCCGCCGTTATAATAATTAAGTTCTACAATCAGTTTCACACATAAAGGTGAG‑aaaa                                                                                                    >  2:1048791/1‑140 (MQ=255)

                                            tGAATACATCCGGACAACCCCGTCGTTTCTTTGTGCAACAGCAGCATATGT‑AACC‑‑ATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATGTGTATGTTATCCAATCAGATTCACACTTCCAGGTGCG‑aaaaaaa                                                                                                 >  2:3339104/1‑140 (MQ=255)

                                                 aCATCCGGACAACCCCGTCGTTTCTTTAAGCAAAACCAGCAAATTT‑AACC‑‑ATAGACAGCGCGCACCGAAAAACAAGCCCCCCCTTATAATATGTATGTTCTCCAATCAGATTAAAACATCCAGGTGCC‑aaaaaaaaacca                                                                                            >  2:3734820/1‑136 (MQ=255)

                                                 aCAACCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGT‑ACCC‑‑ATAGTCAGCGCCCACGGAAACACCTGCACCCCCTTATAATAATTATGTTCACCAAACAGATTCACACTTACAGGTGCG‑aaaaaaaaaccg                                                                                            >  2:1340738/1‑136 (MQ=255)

                                                    tCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGT‑ACCC‑‑ATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCG‑AAAAAAAACCGTAAc                                                                                         <  2:4830859/140‑1 (MQ=255)

                                                    tCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGT‑ACCC‑‑ATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCG‑AAAAAAAACCGTAAc                                                                                         <  1:1301532/140‑1 (MQ=255)

                                                    tCCGGACAACCCCGTCGTTTATTTCTGCAACAGCAGCATATGT‑ACCC‑‑ATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCG‑AAAAAAAACCGTAAc                                                                                         <  1:3413546/140‑1 (MQ=255)

                                                      cGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGT‑ACCC‑‑ATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCG‑AAAAAAAACCGTAAccc                                                                                       <  2:4508515/140‑1 (MQ=255)

                                                      cGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGT‑ACCC‑‑ATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCG‑AAAAAAAACCGTAAccc                                                                                       <  1:1329852/140‑1 (MQ=255)

                                                         aCAACCCCGTCGTTTGTTTGTGCAACACCACCGTATGT‑TACC‑‑CTTGTCAGCGCCCACGGAAACACAAGCCCGCCGTTATAATCTGTATTTTATCCAATCAGATTTAATCTTACAGGTGCG‑AAAAAAAAACGGAAccccac                                                                                    >  2:4330661/1‑138 (MQ=255)

                                                             ccccGTCGTTTCTTTCTGCAACAGCAGCATATGT‑ACCC‑‑ATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTATTCCAGGTTCG‑AAAAAAAAACCTAAACCCTCtttt                                                                                >  2:2884753/1‑140 (MQ=255)

                                                             ccccGTCGTTTCTTTCTGCAACAACAGCAAATGT‑ACCC‑‑ATTGTCACCGCGCCCGGAAACACAAGCCCCCCCTTATAAACTGTATTTTCTACAATCAGATTCAAAATTACAGGTGGG‑TAAAAAAAACGGAACACCTCgttt                                                                                >  2:1843013/1‑140 (MQ=255)

                                                              cccGTCGTTTCTTTCTGCAACAGCAGCATATGT‑ACCC‑‑ATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCG‑AAAAAAAACCGTAACCCCTCttttt                                                                               <  1:2797504/140‑1 (MQ=255)

                                                              cccGTCGTTTCTTTCTGCAACAGCAGCATATGT‑ACCC‑‑ATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCG‑AAAAAAAACCGTAACCCCTCttttt                                                                               <  1:430646/140‑1 (MQ=255)

                                                                cgtcgtTTCTTTCTGCAACAGCAGCATATGT‑ACCC‑‑ATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCG‑AAAAAAAACCGTAACCCCTCTTTTtct                                                                             <  2:5015424/140‑1 (MQ=255)

                                                                     tttctttcTGCAACAGCAGCATATGT‑ACCC‑‑ATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCG‑AAAAAAAACCGTAACCCCTCTTTTTCTCCGaa                                                                        <  1:4500871/140‑1 (MQ=255)

                                                                       tctttctGCAACAGCAGCATATGT‑ACCC‑‑ATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCG‑AAAAAAAACCGTAACCCCTCTTTTTCTCCGAATa                                                                      <  1:4764482/140‑1 (MQ=255)

                                                                       tctttctGCAACAGCAGCATATGT‑ACCC‑‑ATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCG‑AAAAAAAACCGTAACCCCTCTTTTTCTCCGAATa                                                                      <  1:149001/140‑1 (MQ=255)

                                                                           tctGCAACAGCAGCATCTGT‑ACCC‑‑CTTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTCTGGTCTCCAATCAGATTCACTCTTGCAGGTGCG‑AAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACt                                                                  <  2:1474931/140‑1 (MQ=255)

                                                                             tGCAACAGCAGCATATGT‑ACCC‑‑ATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCG‑AAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTg                                                                <  1:199629/140‑1 (MQ=255)

                                                                             tGCAACAGCAGCATAGGT‑ACCC‑‑ATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATTTTCTCCAATCAGATTCACTCGTCCAGGTGCG‑AAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTg                                                                <  2:4719401/140‑1 (MQ=255)

                                                                               cAACAGCAGCATATGT‑ACCC‑‑ATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCG‑AAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCa                                                              >  2:3447127/1‑140 (MQ=255)

                                                                               cAACAGCAGCATATGT‑ACCC‑‑ATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCG‑AAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCa                                                              >  1:3942684/1‑140 (MQ=255)

                                                                               cAACAGCAGCATATGT‑ACCC‑‑ATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCG‑AAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCa                                                              >  1:2768221/1‑140 (MQ=255)

                                                                                aaCAGCAGCATATGT‑ACCC‑‑ATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCG‑AAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCaa                                                             <  1:1408692/140‑1 (MQ=255)

                                                                                   agcagcATATGT‑ACCC‑‑ATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCG‑AAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAata                                                          <  1:1108291/140‑1 (MQ=255)

                                                                                   agcagcATATGT‑ACCC‑‑ATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCG‑AAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAata                                                          <  2:1450544/140‑1 (MQ=255)

                                                                                   agcagcATATGT‑ACCC‑‑ATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCG‑AAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAata                                                          <  1:3566330/140‑1 (MQ=255)

                                                                                    gcagcaTATGT‑ACCC‑‑ATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCG‑AAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAatat                                                         <  1:4142568/140‑1 (MQ=255)

                                                                                     cagcaTATTT‑ACCC‑‑ATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCG‑AAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATc                                                        <  2:4218115/140‑1 (MQ=255)

                                                                                     cagcaTATGT‑ACCC‑‑ATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCG‑AAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATc                                                        <  1:1795418/140‑1 (MQ=255)

                                                                                     cagcaTATGT‑ACCC‑‑ATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCG‑AAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATc                                                        <  1:1896786/140‑1 (MQ=255)

                                                                                     cagcaTATGT‑ACCC‑‑ATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCG‑AAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATc                                                        <  2:402362/140‑1 (MQ=255)

                                                                                     cagcaTATGT‑ACCC‑‑ATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCG‑AAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATc                                                        <  2:2744719/140‑1 (MQ=255)

                                                                                     cagcaTATGT‑ACCC‑‑AGTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCG‑AAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATc                                                        <  2:3515931/140‑1 (MQ=255)

                                                                                     cagaaTTTGG‑ACCC‑‑ATTGTCAGCGCGCAGGGAAACAAATGCCCGCCGTTATAATCTGTATTTTCTCCAATCAGTTTCACTATTCCAGGTGCG‑AAAAAAAACCGTAAACCCTCTTTTTCTCCGAATACACTTGCAAATATc                                                        <  2:3177766/140‑1 (MQ=255)

                                                                                      agcaTATGT‑ACCC‑‑ATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCGAAAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATc                                                        <  2:113987/140‑1 (MQ=255)

                                                                                       gcaTATGT‑ACCC‑‑ATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCG‑AAAAAAAACCGTAACCCCGCTTTTTCTCCGAATACACTTGCCAAGATccc                                                      >  1:3733399/1‑140 (MQ=255)

                                                                                         atatGT‑ACCC‑‑ATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCG‑AAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATCCCCt                                                    <  1:4837016/140‑1 (MQ=255)

                                                                                         atatGT‑ACCC‑‑ATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCG‑AAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATCCCCt                                                    <  1:2838746/140‑1 (MQ=255)

                                                                                          tatGT‑ACCC‑‑ATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCG‑AAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATCCCCtt                                                   <  2:390139/140‑1 (MQ=255)

                                                                                           atGT‑ACCC‑‑ATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCG‑AAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATCCCCttt                                                  <  1:1673740/140‑1 (MQ=255)

                                                                                           atGT‑ACAC‑‑ATTGTCAGCGCGCACGGAAACACATGCCCGCCTTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCG‑AAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATCCCCttt                                                  <  2:4117629/140‑1 (MQ=255)

                                                                                                aCCC‑‑ATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCG‑‑AAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATCCCCTTTAGctc                                             <  2:2577115/140‑1 (MQ=255)

                                                                                                 ccc‑‑ATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCG‑AAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATCCCCTTTAGctc                                             >  2:3744291/1‑140 (MQ=255)

                                                                                                 ccc‑‑ATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCG‑AAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATCCCCTTTAGctc                                             >  2:16251/1‑140 (MQ=255)

                                                                                                   c‑‑ATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCG‑AAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATCCCCTTTAGCTCtt                                           >  2:3953513/1‑140 (MQ=255)

                                                                                                   c‑‑ATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCG‑AAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATCCCCTTTAGCTCtt                                           >  2:4620282/1‑140 (MQ=255)

                                                                                                      aTTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCG‑AAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATCCCCTTTAGCTCTTc                                          >  2:739620/1‑140 (MQ=255)

                                                                                                       ttGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCG‑AAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATCCCCTTTAGCTCTTcc                                         >  2:1532733/1‑140 (MQ=255)

                                                                                                        tGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCAc                                                                                                                      <  2:2099521/63‑1 (MQ=255)

                                                                                                        tGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCAc                                                                                                                      >  1:2099521/1‑63 (MQ=255)

                                                                                                        tGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCG‑AAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATCCCCTTTAGCTCTTccc                                        >  2:5143087/1‑140 (MQ=255)

                                                                                                        tGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCG‑AAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATACCCTTTAGCTCTTccc                                        >  2:2817885/1‑140 (MQ=255)

                                                                                                           cAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAAACACATTCACTCTTCCAGGTGCG‑AAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATCCCCTTTAGCTCTTCCCGCa                                     <  1:1021300/140‑1 (MQ=255)

                                                                                                            aGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCG‑AAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATCCCCTTTAGCTCTTCCCGCAt                                    >  2:3309058/1‑140 (MQ=255)

                                                                                                            aGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCG‑AAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATCCCCTTTAGCTCTTCCCGCAt                                    >  2:90465/1‑140 (MQ=255)

                                                                                                                  cACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCG‑AAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATCCCCTTTAGCTCTTCCCGCATGTGCaa                              <  1:2621245/140‑1 (MQ=255)

                                                                                                                  cACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCG‑AAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATCCCCTTTAGCTCTTCCCGCATGTGCaa                              <  1:722168/140‑1 (MQ=255)

                                                                                                                  cACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCG‑AAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATCCCCTTTAGCTCTTCCCGCATGTGCaa                              <  2:662002/140‑1 (MQ=255)

                                                                                                                               gCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCG‑AAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATCCCCTTTAGCTCTTCCCGCATGTGCAATCACCTTTCATTg                 >  1:2211863/1‑140 (MQ=255)

                                                                                                                               gCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCG‑AAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATCCCCTTTAGCTCTTCCCGCATGTGCAATCACCTTTCATTg                 >  2:4197267/1‑140 (MQ=255)

                                                                                                                                 ccgccgTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCG‑AAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATCCCCTTTAGCTCTTCCCGCATGTGCAATCACCTTTCATTGtt               >  2:4585020/1‑140 (MQ=255)

                                                                                                                                 ccgccgTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCG‑AAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATCCCCTTTAGCTCTTCCCGCATGTGCAATCACCTTTCATTGtt               >  2:2673343/1‑140 (MQ=255)

                                                                                                                                 ccgccgTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCG‑AAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATCCCCTTTAGCTCTTCCCGCATGTGCAATCACCTTTCATTGtt               >  1:4279565/1‑140 (MQ=255)

                                                                                                                                     cgTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCG‑AAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATCCCCTTTAGCTCTTCCCGCATGTGCAATCACCTTTCATTGttttat           <  2:612194/140‑1 (MQ=255)

                                                                                                                                             tCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCG‑AAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATCCCCTTTAGCTCTTCCCGCATGTGCAATCACCTTTCATTGttttatttttttat   <  1:2322521/140‑1 (MQ=255)

                                                                                                                                             tCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCG‑AAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATCCCCTTTAGCTCTTCCCGCATGTGCAATCACCTTTCATTGttttatttttttat   <  1:2363228/140‑1 (MQ=255)

                                                                                                                                             tCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCG‑AAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATCCCCTTTAGCTCTTCCCGCATGTGCAATCACCTTTCATTGttttatttttttat   <  1:828495/140‑1 (MQ=255)

                                                                                                                                             tCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCG‑AAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATCCCCTTTAGCTCTTCCCGCATGTGCAATCACCTTTCATTGttttatttttttat   <  1:4881540/140‑1 (MQ=255)

                                                                                                                                              cTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCG‑AAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATCCCCTTTAGCTCTTCCCGCATGTGCAATCACCTTTCATTGttttatttttttatt  <  2:1656705/140‑1 (MQ=255)

TCGTCTAGGCTGCATTCCTTGATCCCTACGTCTTTAAAGTTGTCTGAATACATCCGGACAACCCCGTCGTTTCTTTCTGCAACAGCAGCATATGT‑ACCC‑‑ATTGTCAGCGCGCACGGAAACACATGCCCGCCGTTATAATCTGTATGTTCTCCAATCAGATTCACTCTTCCAGGTGCG‑AAAAAAAACCGTAACCCCTCTTTTTCTCCGAATACACTTGCAAATATCCCCTTTAGCTCTTCCCGCATGTGCAATCACCTTTCATTGTTTTATTTTTTTATT  >  NZ_CP034943/3746823‑3747101

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`