BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	3,790,798	C→A	5.6%	E191D (GAG→GAT)	EO946_RS19525 ←	BglG family transcription antiterminator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	3,790,798	0	C	A	5.6%	760.5 / 2.8	277	E191D (GAG→GAT)	EO946_RS19525	BglG family transcription antiterminator
Reads supporting (aligned to +/- strand): ref base C (28/212); new base A (25/2); total (63/214)
Fisher's exact test for biased strand distribution p-value = 2.69e-18
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 282 total aligned reads displayed.

CCGGGAAAAGAGATACATAGTTTTGCGTTCTGATCCGTTTACAGGCGATAGCGATATGGATGATGAGGTTATTCAGTCCCAAGTTGGACAGAGGGATGCGGTCGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTT‑CAT‑TTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTTCTCTTTCATCGACAATGTACTCCGCCATACAGTACCTCATCTGCACCTCGTCCCCGCGCAGCTTGAAGCCGTAATTTGGGC  >  NZ_CP034943/3790659‑3790937

   ggAAAAGAGATACATAGTTTTGCGTTCTGATCCGTTTACAGGCGATAGCGATATGGATGATGAGGTTATTCAGTCCCAAGTTGGACAGAGGGATGCGGTCGTGTTTAATTTTTTTAAGGATGTCGGACAGGCTGATATCg                                                                                                                                            >  2:1477142/1‑140 (MQ=255)

                  aGTTTTGCGTTCTGATCCGTTTACAGGCGATAGAGATATGGATGATGAGGTTATTCAGTCCCAAGTTGGACAGAGGGATGCGGTCGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTT‑CAT‑TTGGc                                                                                                                           <  1:5123074/140‑1 (MQ=255)

                   gTTTTGCGTTCTGATCCGTTTACATGCGATAGAGATATGGATGATGAGGTTATTCAGTCCCAAGTTGGACAGAGGGATGCGGTCGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTT‑CAT‑TTGGCa                                                                                                                          <  2:4812382/140‑1 (MQ=255)

                   gTTTTGCGTTCTGATCCGTTTACAGGCGATAGCGATATGGATGATGAGGTTATTCAGTCCCAAGTTGGACAGAGGGATGCGGTCGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTT‑CAT‑TTGGCa                                                                                                                          <  2:1378401/140‑1 (MQ=255)

                   gTTTTGCGTTCTGATCCGTTTACAGGCGATAGCGATATGGATGATGAGGTTATTCAGTCCCAAGTTGGACAGAGGGATGCGGTCGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTT‑CAT‑TTGGCa                                                                                                                          <  1:1342314/140‑1 (MQ=255)

                   gTTTTGCGTTCTGATCCGTTTACAGGCGATAGCGATATGGATGATGAGGTTATTCAGTCCCAAGTTGGACAGAGGGATGCGGTCGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTT‑CAT‑TTGGCa                                                                                                                          <  1:1555762/140‑1 (MQ=255)

                   gTTTTGCGTTCTGATCCGTTTACAGGCGATAGCGATATGGATGATGAGGTTATTCAGTCCCAAGTTGGACAGAGGGATGCGGTCGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTT‑CAT‑TTGGCa                                                                                                                          <  1:25295/140‑1 (MQ=255)

                    ttttGCGTTCTGATCCGTTTACAGGCGATAGCGATATGGATGATGAGGTTATTCAGTCCCAAGTTGGACAGAGGGATGCGGTCGTGTTTTATTTTTTTTAGGATGGGGGGACGCGTTATATAGATTTATT‑GAt‑ttttgaa                                                                                                                         >  1:280732/1‑135 (MQ=255)

                         cGTTCTGATCCGTTTACAGGCGATAGCGATATGGATGATGAGGTTATTCAGTCCCAAGTTGGACAGAGGGATGCGGTCGTGTTTGATTTTTTTTATGTTTGGGGGACGGGAGTTCTAGATTTGTT‑AATATTGGTaaaaat                                                                                                                     >  1:2011915/1‑138 (MQ=255)

                            tCTGATCCGTTTACAGGCGATAGCGATCTGGATGATGAGGTTATTCAGTCCCAAGGTGGACAGAGGGATGCGGTCGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTT‑CAT‑TTGGCAAAATATCAg                                                                                                                 <  1:1192482/140‑1 (MQ=255)

                              tGATCCGTTTACAGGCGATAGCGATATGGATGATGAGGTTATTCAGTCCCAAGTTGGACAGAGGGATGCGGTCGTGTTTAAATTTTTTGAGGAAGGCGGCCAGGATGTTCACGATTTATTGGAT‑TTGGGaaaaatatggc                                                                                                                >  1:1628932/1‑133 (MQ=255)

                                 tcagTTTACAGGCGATAGCGATATGGATGATGAGGTTATTCAGTCCCAAGTTGGACAGAGGGATGCGGTCGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTT‑CAT‑TTGGCAAAATATCAGCCttt                                                                                                            <  1:3654831/137‑1 (MQ=255)

                                     tttACAGGCGATAGCGATATGGATGATGAGGTTATTCAGTCCCAAGTTGGACAGAGGGATGCGGTCGTTTTTAATTTTTTTAAGTATGGCGGAACGGATGATATAGAATTATT‑GAT‑TTTGGAAAATATCAGCCATttgat                                                                                                        >  2:1163996/1‑140 (MQ=255)

                                      tgaCAGGCGATAGCGAGATGGATGATGAGGGTATTCCGTCCCAAGTTGGACAGAGGGATGCGGTCGATTTACATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTT‑CAG‑TGGGCAAAATATCAGCCTTttcatt                                                                                                       <  1:1601056/138‑1 (MQ=255)

                                          aGGCGATAGCGATATGGATGATGAGGTTATTCAGTCCCAAGTTGGACAGAGGGATGCGGTCGTTTTTGATTTTTTTTAGGATTGGGGTAACGATGATATCGATTTATT‑AAT‑TTTGGAAAATATAATCCATTTGATTgaga                                                                                                   >  2:3105708/1‑140 (MQ=255)

                                          aGGCGATAGCGATATGGATGATGAGGTTATTCAGTCCCAAGTTGGACAGAGGGATGCGGTCGTGTTTAATTTTTTTAAGGATTGCGGAACCGATTATCTCGATTTATT‑GAT‑TTTTGAAAAAATAAACCTTttaaatcaaa                                                                                                   >  2:2195515/1‑138 (MQ=255)

                                            gcgatagcgataTGGATGATGAGGTTATTCAGTCCCAAGTTGGACAGAGGGATGCGGTCGTTTTTGATTTTTTTAAGGATGGGGGAACCGATGTACTAGTTTTGTT‑CAT‑TTTTGAAAAAATAAGCCTGTTAATTAAGAAc                                                                                                 >  2:231812/1‑140 (MQ=255)

                                               atagcgataTGGATGATGAGGTTATTCAGTCCCAAGTTGGACAGAGGGATGCGGTCGTTTTTTATTTTTTTGAGTATGGCGGAACGGATGATATCGAATTATT‑AAG‑TTTTGAAAATAACAGCCATTTAATTAAGAACaaa                                                                                              >  2:4259115/1‑138 (MQ=255)

                                               atagcgataTGGATGATGAGGTTATTCAGTCCCAAGTTGGACAGAGGGATGCGGTCGTTTTTTAATTTTTTTAGGATGTCGGAAACGATGATATCGAATTATT‑CAT‑TTTGGAAAAAATCAGACGTTTAATTTAGAAAAta                                                                                              >  2:3920273/1‑139 (MQ=255)

                                               atagcgataTGGATGATGAGGTTATTCAGTCCCAAGTTGGACAGAGGGATGCGGTCGTTTTTTAATTTTTTGAGGATTGGGGAACGGAATAATACGAATTATT‑AAT‑TTTGCAAAAAAAAACCCATTTTATTAAGaaaaaa                                                                                              >  2:4740759/1‑136 (MQ=255)

                                               atagcgataTGGATGATGAGGTTATTCAGTCCCAAGTTGGACAGAGGGATGCGGTCGTTTTTAATTTTTTTGCGGTTTGGGGGAAGGATGATATCGAGTTGGT‑GAT‑TTGGGAAAATATAAGCCTTTTGATTAATAACaac                                                                                              >  2:1782105/1‑138 (MQ=255)

                                               atagcgataTGGATGATGAGGTTATTCAGTCCCAAGTTGGACAGAGGGATGCGGTCGTGTTTGATTTTTTTGATGATGGCGGAAAGGCTGATATCGATTTGAT‑CAT‑TTGGCAAAATATCAGCCTTTTCATTAAGaaaaac                                                                                              >  2:5079249/1‑136 (MQ=255)

                                               atagcgataTGGATGATGAGGTTATTCAGTCCCAAGTTGGACAGAGGGATGCGGTCGTGTTTGATTTTTTTAAGGATGGCGGAACCGATTATATACATTTCTT‑AAA‑TTTGGAAAAAAAAAGCCATTTGATTCAgtaaacc                                                                                              >  2:5179566/1‑134 (MQ=255)

                                               atagcgataTGGATGATGAGGTTATTCAGTCCCAAGTTGGACAGAGGGATGCGGTCGTGTTTGATTTTTTTAAAGATGGCGGAAACGAATATCTCGATTTATT‑CAT‑TTTGGAAAAAATCAGCCTTTTAATTGAGaaaaac                                                                                              >  2:4064138/1‑136 (MQ=255)

                                               atagcgataTGGATGATGAGGTTATTCAGTCCCAAGTTGGACAGAGGGATGCGGTCGTGTTTAATTTTTTTGAGGATTGCGGAAAGGATGTTTTCGTTTTATT‑GAT‑TTTGCAAAAGATAATCATTTTGATTAATAAAATc                                                                                              >  2:3487857/1‑140 (MQ=255)

                                               atagcgataTGGATGATGAGGTTATTCAGTCCCAAGTTGGACAGAGGGATGCGGTCGTGTTTAATTTTTTTAAGGCGGGCGGCCCCGATGTTGTGTATTTCTT‑CAA‑TTGGGCCTATATCAGGCGGTTAATTGAGAACaga                                                                                              >  2:4508541/1‑138 (MQ=255)

                                               atagcgataTGGATGATGAGGTTATTCAGTCCCAAGTTGGACAGAGGGATGCGGTCGTGGTTGATTTTTTTGATGTTGGGGGGACGGGTGATGTGGATTTGGT‑AAA‑ATGTGAATATATAAGCCTGTTGATTGAGAACaga                                                                                              >  2:3532095/1‑138 (MQ=255)

                                                tagcgataTGGATGATGAGGTTATTCAGTCCCAAGTTGGACAGAGGGATGCGGTCGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTT‑CAT‑TTGGCAAAATATCAGCCTTTTCATTCAGAACATCa                                                                                             <  1:4161334/140‑1 (MQ=255)

                                                tagcgataTGGATGATGAGGTTATTCAGTCCCAAGTTGGACAGAGGGATGCGGTCGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTT‑CAT‑TTGGCAAAATATCAGCCTTTTCATTCAGAACATCa                                                                                             <  1:1500769/140‑1 (MQ=255)

                                                        tGGATGATGAGGTTATTCAGTCCCAAGTTGGACAGAGGGATGCGGTCGTTGTTGATTTTTTTTAGGATTGCGGAAAGGATGTTCTAGATTTCTT‑AAT‑TTTTGAAAATATAAGCCTTTTAATTGAGAAAATaaaaatcagt                                                                                     >  2:2696644/1‑133 (MQ=255)

                                                            tgatgaGGTTATTCAGTCCCAAGTTGGACAGAGGGATGCGGTCGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTT‑CAT‑TTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTtct                                                                                 <  1:2128191/140‑1 (MQ=255)

                                                            tgatgaGGTTATTCAGTCCCAAGTGGGACAGAGGGATGCGGTCGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTT‑CAT‑TTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTtct                                                                                 <  2:2808894/140‑1 (MQ=255)

                                                                   gTTATTCAGTCCCAAGTTGGACAGAGGGATGCGGTCGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTT‑CAT‑TTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTTCTCTTTCAt                                                                          <  1:3730240/140‑1 (MQ=255)

                                                                   gTTATTCAGTCCCAAGTTGGACAGAGGGATGCGGTCGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTT‑CAT‑TTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTTCTCTTTCAt                                                                          <  1:390501/140‑1 (MQ=255)

                                                                   gTTATTCAGTCCCAAGTTGGACAGAGGGATGCGGTCGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTT‑CAT‑TTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTTCTCTTTCAt                                                                          <  1:3950845/140‑1 (MQ=255)

                                                                   gTTATTCAGTCCCAAGTTGGACAGAGGGATGCGGTCGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTT‑CAT‑TTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTTCTCTTTCAt                                                                          <  2:3769619/140‑1 (MQ=255)

                                                                   gTTATTCAGTCCAAAGTTGGACAGAGGGATGCGGTCGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTT‑CAT‑TTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTTCTCTTTCAt                                                                          <  2:4031708/140‑1 (MQ=255)

                                                                        tCAGTCCCAAGTTGGACAGAGGGATGCGGTCGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTT‑CAT‑TTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTTCTCTTTCATCGACa                                                                     <  1:75368/140‑1 (MQ=255)

                                                                        tCAGTCCCAAGTTGGACAGAGGGATGCGGTCGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTT‑CAT‑TTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTTCTCTTTCATCGACa                                                                     <  1:74154/140‑1 (MQ=255)

                                                                        tCAGTCCCAAGTTGGACAGAGGGATGCGGTCGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTT‑CAT‑TTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTTCTCTTTCATCGACa                                                                     <  1:1070602/140‑1 (MQ=255)

                                                                        tCAGTCCCAAGTTGGACAGAGGGATGCGGTCGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTT‑CAT‑TTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTTCTCTTTCATCGACa                                                                     <  1:1702623/140‑1 (MQ=255)

                                                                        tCAGGCCCAAGTTGGACAGAGGGATGCGGTCGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTT‑CAT‑TTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTTCTCTTTCATCGACa                                                                     <  1:93293/140‑1 (MQ=255)

                                                                         cAGTCCCAAGTTGGACAGAGGGATGCGGTCGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTT‑CAT‑TTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTTCTCTTTCATCGAcct                                                                    <  1:157441/140‑3 (MQ=255)

                                                                             gccAAGTTGTAAAGAGGGATGCGGTCGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTT‑CAT‑TTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTTCTCTTTCATCGACAATGTa                                                                <  2:4002675/139‑1 (MQ=255)

                                                                             cccAAGTTGGACAGAGGGATGCGGTCGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTT‑CAT‑TTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTTCTCTTTCATCGACAATGTa                                                                <  1:4568292/140‑1 (MQ=255)

                                                                             cccAAGTTGGACAGAGGGATGCGGTCGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTT‑CAT‑TTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTTCTCTTTCATCGACAATGTa                                                                <  2:156441/140‑1 (MQ=255)

                                                                             cccAAGTTGGACAGAGGGATGCGGTCGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTT‑CAT‑TTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTTCTCTTTCATCGACAATGTa                                                                <  2:2505205/140‑1 (MQ=255)

                                                                             cccAAGTTGGACAGAGGGATGCGGTCGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTT‑CAT‑TTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTTCTCTTTCATCGACAATGTa                                                                <  1:2260182/140‑1 (MQ=255)

                                                                             cccAAGTTGGACAGAGGGATGCGGTCGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTT‑CAT‑TTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTTCTCTTTCATCGACAATGTa                                                                <  2:2175798/140‑1 (MQ=255)

                                                                             cccAAGTTGGACAGAGGGATGCGGTCGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTT‑CAT‑TTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTTCTCTTTCATCGACAATGTa                                                                <  1:2049325/140‑1 (MQ=255)

                                                                             cccAAGTTGGACAGAGGGATGCGGTCGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTT‑CAT‑TTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTTCTCTTTCATCGACAATGTa                                                                <  1:628000/140‑1 (MQ=255)

                                                                             cccAAGTTGGACAGAGGGATGCGGTCGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTT‑CAT‑TTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTTCTCTTTCATCGACAATGTa                                                                <  1:4064138/140‑1 (MQ=255)

                                                                             cccAAGTTGGACAGAGGGATGCGGTCGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTT‑CAT‑TTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTTCTCTTTCATCGACAATGTa                                                                <  1:4740759/140‑1 (MQ=255)

                                                                             cccAAGTTGGACAGAGGGACGCGGTCGTTTTTCATTTTTTTCCTGCTCGCGGAACGGATGATCTCGATTTCTT‑CAT‑TGGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGGTTCTCTTTCATCGACAATGTa                                                                <  2:2830748/140‑1 (MQ=255)

                                                                             ccaaatttGGACAGAGGGATGCGGTCGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTT‑CAT‑TTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTTCTCTTTCATCGACAATGTa                                                                <  2:3259997/134‑1 (MQ=255)

                                                                             ccaaaGTTGGACAGAGGTATGCGGTCGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTT‑CAT‑TTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTTCTCTTTCATCGACAATGTa                                                                <  1:314857/137‑1 (MQ=255)

                                                                             cacAAGTTGGACAGAGGGATGCGGTCGTTTCTCATTTTTTTCAGGAGGGCGGAACGGATGATCTCGATTTCTT‑CAT‑TTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTTCTCTTTCATCGACAATGTa                                                                <  1:3515145/138‑1 (MQ=255)

                                                                               cAAGTTGGACAGAGGGATGCGGTCGTTTTTCATTTTTTTCAGGCTGGCGGAACGGATGATCTCGATTTCTT‑CAT‑TTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTTCTCTTTCATCGACAATGTACt                                                              <  2:3009520/140‑1 (MQ=255)

                                                                               cAAGTTGGACAGAGGGATGCGGTCGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTT‑CAT‑TTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTTCTCTTTCATCGACAATGTACt                                                              <  2:4147698/140‑1 (MQ=255)

                                                                                 aGTTGGACAGAGGGATGCGGTCGTGGTTGATTTTTTTTAGGTTGGCGGAAACGTTGGTAGCCAGTTATT‑CAG‑TTGGCAGAATATGGCGCGTTTCAGTAATAACATGCAGCGCCGGGTGGGTTTCATCGACAATGttaggc                                                            >  2:1287801/1‑135 (MQ=255)

                                                                                  gTTGGACAGAGGGATGCGGTCGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTT‑CAT‑TTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTTCTCTTTCATCGACAATGTACTCCg                                                           <  1:4304138/140‑1 (MQ=255)

                                                                                  gTTGGACAGAGGGATGCGGTCGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTT‑CAT‑TTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTTCTCTTTCATCGACAATGTACTCCg                                                           <  1:2581362/140‑1 (MQ=255)

                                                                                  gTTGGACAGAGGGATGCGGTCGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTT‑CAT‑TTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTTCTCTTTCATCGACAATGTACTCCg                                                           <  1:1908830/140‑1 (MQ=255)

                                                                                  gTTGGACAGAGGGATGCGGTCGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTT‑CAT‑TTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTTCTCTTTCATCGACAATGTACTCCg                                                           <  2:4642480/140‑1 (MQ=255)

                                                                                  gTTGGACAGAGGGATGCGGTCGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTT‑CAT‑TTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTTCTCTTTCATCGACAATGTACTCCg                                                           <  1:1752044/140‑1 (MQ=255)

                                                                                  gTTGGACAGAGGGATGCGGTCGTTTTTCATTTTTTTCAGGAGGGCGGAACGGATGATCTCGATTTCTT‑CAT‑TGGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTTCTCTTTCATCGACAATGTACTCCg                                                           <  1:771120/140‑1 (MQ=255)

                                                                                  cagggACAGAGGGATGCGGTCGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTT‑CAT‑TTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTTCTCTTTCATCGACAATGTACTCCg                                                           <  1:2381929/137‑1 (MQ=255)

                                                                                   ttGGACAGAGGGATGCGGTCGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTT‑CAT‑TTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTTCTCTTTCATCGACAATGTACTCCGc                                                          <  1:2877393/140‑1 (MQ=255)

                                                                                    tgtaCAGAGGGATGCGGTCGTTTTTCATTTTTTTCATGATGGCGGAACGGATGATCTCGATTTCTT‑CAT‑TTGGCAAAATATCAGCCTTTTAATTCAGAACATCAATCTCCGTTTCTCTTTCATCGACAATGTACTACGcc                                                         <  1:1841081/137‑1 (MQ=255)

                                                                                    tGGACATAGGGATGCGGTCGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTT‑CAT‑TTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTTCTCTTTCATCGACAATGTACTCCGcc                                                         <  2:4392439/140‑1 (MQ=255)

                                                                                    tGGACAGAGGGATGCGGTAGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTT‑CAT‑TTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTTCTCTTTCATCGACAATGTACTCCGcc                                                         <  2:3657631/140‑1 (MQ=255)

                                                                                     tgACAGAGGGATGCGGTCGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGAAATCGATTTCTT‑CAT‑TTGTCAAAATATCAGCCTTTTCATACAGAACATCAAACTCCTTTTCTCTTTCATCGACAATGTACTCCGCCa                                                        <  1:3815928/139‑1 (MQ=255)

                                                                                     ggACAGAGGGATGCGGTCGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTT‑CAT‑TTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTTCTCTTTCATCGACAATGTACTCCGCCa                                                        <  1:3568150/140‑1 (MQ=255)

                                                                                        cAGAGGGATGCGGTCGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTT‑CAT‑TTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTTCTCTTTCATCGACAATGTACTCCGCCATAc                                                     <  2:1390093/140‑1 (MQ=255)

                                                                                        cAGAGGGATGCGGTCGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTT‑CAT‑TTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTTCTCTTTCATCGACAATGTACTCCGCCATAc                                                     <  1:992612/140‑1 (MQ=255)

                                                                                        cAGAGGGATGCGGTCGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTT‑CAT‑TTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTTCTCTTTCATCGACAATGTACTCCGCCATAc                                                     <  1:1008302/140‑1 (MQ=255)

                                                                                        cAGAGGGATGCGGTCGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTT‑CAT‑TTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTTCTCTTTCATCGACAATGTACTCCGCCATAc                                                     <  2:3153272/140‑1 (MQ=255)

                                                                                        cAGAGGGATGCGGTCGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTT‑CAT‑TTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTTCTCTTTCATCGACAATGTACTCCGCCATAc                                                     <  1:4882421/140‑1 (MQ=255)

                                                                                        cAGAGGGATGCGGTCGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTT‑CAT‑TTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTTCTCTTTCATCGACAATGTACTCCGCCATAc                                                     <  1:4216527/140‑1 (MQ=255)

                                                                                        cAGAGGGATGCGGTCGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTT‑CAT‑TTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTTCTCTTTCATCGACAATGTACTCCGCCATAc                                                     <  2:615414/140‑1 (MQ=255)

                                                                                        cAGAGGGATGAGGTCGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTT‑CAT‑TTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTTCTCTTTCATCGACAATGTACTCCGCCATAc                                                     <  2:5130872/140‑1 (MQ=255)

                                                                                          gagGGATGCGGTCGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTT‑CAT‑TTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTTCTCTTTCATCGACAATGTACTCCGCCATACAg                                                   <  1:4990665/140‑1 (MQ=255)

                                                                                             ggATGCGGTCGTTTTTCATTTTTTTCAGGATGGCGGAACTGATGATCTCGATTTCTT‑CAT‑TTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTTCTCTTTCATCGACAATGTACTCCGCCATACAGTAc                                                <  1:4102969/140‑1 (MQ=255)

                                                                                             ggATGCGGTCGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTT‑CAT‑TTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTTCTCTTTCATCGACAATGTACTCCGCCATACAGTAc                                                <  1:558804/140‑1 (MQ=255)

                                                                                             ggATGCGGTCGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTT‑CAT‑TTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTTCTCTTTCATCGACAATGTACTCCGCCATACAGTAc                                                <  1:1249045/140‑1 (MQ=255)

                                                                                             ggATGCGGTCGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATGTCTT‑CAT‑TTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTTCTCTTTCATCGACAATGTACTCCGCCATACAGTAc                                                <  2:3302503/140‑1 (MQ=255)

                                                                                               ttGCGGTTGTTTTTCATTTTTTTCATGAGGGCGTAACGTTTGATCTCGATTTCTT‑CAT‑TTCTCAAATTATCATCCTTTTGATTCATAACAACAATCTCCTTTTCTCTTTCATCGTCAATGTACTCGGCCATACAGTACCt                                              <  2:1716043/139‑1 (MQ=255)

                                                                                                          tttCATTTTTTTCAGGATGGCGGAACGGATTATCTCGATTTCTT‑CAT‑TTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTTCTCTTTCATCGACAATGTACTCCGCCATACAGTACCTCATCTGCACCt                                   <  1:3106014/140‑1 (MQ=255)

                                                                                                          tttCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTT‑CAT‑TTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTTCTCTTTCATCGACAATGTACTCCGCCATACAGTACCTCATCTGCACCt                                   <  1:4081738/140‑1 (MQ=255)

                                                                                                          tttCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTT‑CAT‑TTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTTCTCTTTCATCGACAATGTACTCCGCCATACAGTACCTCATCTGCACCt                                   <  1:2195515/140‑1 (MQ=255)

                                                                                                          tttCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTT‑CAT‑TTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTTCTCTTTCATCGACAATGTACTCCGCCATACAGTACCTCATCTGCACCt                                   <  1:1418879/140‑1 (MQ=255)

                                                                                                              aTTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTT‑CAT‑TTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTTCTCTTTCATCGACAATGTACTCCGCCATACAGTACCTCATCTGCACCTCGTc                               <  1:3287342/140‑1 (MQ=255)

                                                                                                                    ttCAGGATGGCGGAACGGATGATCTCGATTTCTT‑CAT‑TTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTTCTCTTTCATCGACAATGTACTCCGCCATACAGTACCTCATCTGCACCTCGTCCCcgcg                         <  2:1917818/140‑1 (MQ=255)

                                                                                                                    ttCAGGATGGCGGAACGGATGATCTCGATTTCTT‑CAT‑TTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTTCTCTTTCATCGACAATGTACTCCGCCATACAGTACCTCATCTGCACCTCGTCCCcgcg                         <  1:4835941/140‑1 (MQ=255)

                                                                                                                    ttCAGGATGGCGGAACGGATGATCTCGATTTCTT‑CAT‑TTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTTCTCTTTCATCGACAATGTACTCCGCCATACAGTACCTCATCTGCACCTCGTCCCcgcg                         <  1:3489417/140‑1 (MQ=255)

                                                                                                                    ttCAGGATGGCGGAACGGATGATCTCGATTTCTT‑CAT‑TTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGGTTCTCTTTCATCGACAATGTACTCCGCCATACAGTACCTCATCTGCACCTCGTCCCcgcg                         <  2:2069930/140‑1 (MQ=255)

                                                                                                                                 aaCGGATGATCTCGATTTCTT‑CAT‑TTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTTCTCTTTCATCGACAATGTACTCCGCCATACAGTACCTCATCTGCACCTCGTCCCCGCGCAGCTTGAAGCCg            <  1:2696644/140‑1 (MQ=255)

                                                                                                                                      atgatCTCGATTTCTT‑CAT‑TTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTTCTCTTTCATCGACAATGTACTCCGCCATACAGTACCTCATCTGCACCTCGTCCCCGCGCAGCTTGAAGCCGTAAtt       >  2:4844227/1‑140 (MQ=255)

                                                                                                                                           ctcGATTTCTT‑CAT‑TTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTTCTCTTTCATCGACAATGTACTCCGCCATACAGTACCTCATCTGCACCTCGTCCCCGCGCAGCTTGAAGCCGTAATTTGGGc  <  1:1168755/140‑1 (MQ=255)

                                                                                                                                           ctcGATTTCTT‑CAT‑TTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTTCTCTTTCATCGACAATGTACTCCGCCATACAGTACCTCATCTGCACCTCGTCCCCGCGCAGCTTGAAGCCGTAATTTGGGc  <  2:742300/140‑1 (MQ=255)

CCGGGAAAAGAGATACATAGTTTTGCGTTCTGATCCGTTTACAGGCGATAGCGATATGGATGATGAGGTTATTCAGTCCCAAGTTGGACAGAGGGATGCGGTCGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTT‑CAT‑TTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTTCTCTTTCATCGACAATGTACTCCGCCATACAGTACCTCATCTGCACCTCGTCCCCGCGCAGCTTGAAGCCGTAATTTGGGC  >  NZ_CP034943/3790659‑3790937

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`