BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	3,790,814	G→T	9.8%	P186Q (CCA→CAA)	EO946_RS19525 ←	BglG family transcription antiterminator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	3,790,814	0	G	T	9.8%	785.2 / 35.2	295	P186Q (CCA→CAA)	EO946_RS19525	BglG family transcription antiterminator
Reads supporting (aligned to +/- strand): ref base G (29/231); new base T (32/2); total (61/234)
Fisher's exact test for biased strand distribution p-value = 1.52e-23
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 295 total aligned reads displayed.

AGTTTTGCGTTCTGATCCGTTTACAGGCGATAGCGATATGGATGATGAGGTTATTCAGTCCCAAGTTGGACAGAGGGATGCGGTCGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTT‑CA‑T‑TTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTTCTCTTTCATCGACAATGTACTCCGCCATACAGTACCTCATCTGCACCTCGTCCCCGCGCAGCTTGAAGCCGTAATTTGGGCGTGTTTCCATGACGA  >  NZ_CP034943/3790677‑3790952

 gTTTTGCGTTCTGATCCGTTTACATGCGATAGAGATATGGATGATGAGGTTATTCAGTCCCAAGTTGGACAGAGGGATGCGGTCGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTT‑CA‑T‑TTGGCa                                                                                                                                         <  2:4812382/140‑1 (MQ=255)

 gTTTTGCGTTCTGATCCGTTTACAGGCGATAGCGATATGGATGATGAGGTTATTCAGTCCCAAGTTGGACAGAGGGATGCGGTCGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTT‑CA‑T‑TTGGCa                                                                                                                                         <  1:2169724/140‑1 (MQ=255)

 gTTTTGCGTTCTGATCCGTTTACAGGCGATAGCGATATGGATGATGAGGTTATTCAGTCCCAAGTTGGACAGAGGGATGCGGTCGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTT‑CA‑T‑TTGGCa                                                                                                                                         <  1:1555762/140‑1 (MQ=255)

  ttttGCGTTCTGATCCGTTTACAGGCGATAGCGATATGGATGATGAGGTTATTCAGTCCCAAGTTGGACAGAGGGATGCGGTCGTGTTTGATTTTTTTTAGTTTGGCGGACCGGCTGTTGTCGATTTGTT‑AA‑G‑TTGGcga                                                                                                                                        >  1:3857567/1‑138 (MQ=255)

    ttGCGTTCTGATCCGTTTACAGGCGATAGCGATATGGATGATGAGGTTATTCAGTCCCAAGTTGGACAGAGGGATGCGGTCGTGTTTTTTTTTTTTGATGATTGGGGGACGGATTATATCGCTTTGTT‑AA‑T‑TTTGGaaaa                                                                                                                                      >  1:2389955/1‑140 (MQ=255)

    ttGCGTTCTGATCCGTTTACAGGCGATAGCGATATGGATGATGAGGTTATTCACTCCCAAGTTGGACAGAGGGATGCGGTCGTGTTTGAAATTTTTCAGGGGGGCGGAACCGAAGATGGCGAATTGTT‑CA‑T‑TTGGCaaaa                                                                                                                                      >  2:3923812/1‑140 (MQ=255)

       cGTTCTGATCCGTTTACAGGCGATAGCGATATGGATGATGAGGTTATTCAGTCCCAAGTTGGACAGAGGGATGCGGTCGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTT‑CA‑T‑TTGGCAAAatat                                                                                                                                   <  2:2598344/140‑1 (MQ=255)

       cGTTCTGATCCGTTTACAGGCGATAGCGATATGGATGATGAGGTTATTCAGTCCCAAGTTGGACAGAGGGATGCGGTCGTGTTTGATTTTTTTTATGTTTGGGGGACGGGAGTTCTAGATTTGTT‑AA‑TATTGGTaaaaat                                                                                                                                    >  1:2011915/1‑138 (MQ=255)

       cGTTCTGATCCGTTTACAGGCGATAGCGATATGGATGATGAGGTTATTCAGTCCCAAGTTGGACAGAGGGATGCGGTCGTGTTTCAATTTTTTGAGGGAGGGGGTAACGATTATCTAGATTTGTT‑AA‑T‑TTTGGAAAaaat                                                                                                                                   >  2:622604/1‑140 (MQ=255)

            tGATCCGTTTACAGGCGATAGCGATATGGATGATGAGGTTATTCAGTCCCAAGTTGGACAGAGGGATGCGGTCGTGTTTAAATTTTTTGAGGAAGGCGGCCAGGATGTTCACGATTTATTGGA‑T‑TTGGGaaaaatatggc                                                                                                                               >  1:1628932/1‑133 (MQ=255)

               tcagTTTACAGGCGATAGCGATATGGATGATGAGGTTATTCAGTCCCAAGTTGGACAGAGGGATGCGGTCGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTT‑CA‑T‑TTGGCAAAATATCAGCCttt                                                                                                                           <  1:3654831/137‑1 (MQ=255)

               tCCGTTTACAGGCGATAGCGATATGGATGATGAGGTTATTCAGTCCCAAGTTGGACAGAGGGATGCGGTCGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTT‑CA‑T‑TTGGCAAAATATCAGCCttt                                                                                                                           <  2:4716431/140‑1 (MQ=255)

               tCCGTTGACAGGCGATAGCGATATGGATGATGAGGTTATTCAGTCCCAAGTTGGACAGAGGGATGCGGTCGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTT‑CA‑T‑TTGGCAAAATATCAGCCttt                                                                                                                           <  1:652968/140‑1 (MQ=255)

                             atagcgataTGGATGATGAGGTTATTCAGTCCCAAGTTGGACAGAGGGATGCGGTGTTTTTTGATATTTTTAAGGATGGCGGAACGGATTATATCTATTTGTT‑AA‑T‑TTTGGAAAATATCATCCTGTTCATTAAAAACaaa                                                                                                             >  2:5118091/1‑138 (MQ=255)

                             atagcgataTGGATGATGAGGTTATTCAGTCCCAAGTTGGACAGAGGGATGCGGTCGTTTTTGCTTTTTTTGAGGGTGGCGGAACCGCTGATCTCGATGTATT‑CA‑T‑TTGGGGAAATATAAGCGCTttgattcatcacaac                                                                                                             >  2:4053302/1‑133 (MQ=255)

                             atagcgataTGGATGATGAGGTTATTCAGTCCCAAGTTGGACAGAGGGATGCGGTCGTTTTTGATTTTTTTAAGGATGGCGGGACCGAAGATATAGATTTGTT‑AA‑T‑TTTTCAAAAAATAAGCAGTTTGATTCAGAACATc                                                                                                             >  2:375528/1‑140 (MQ=255)

                             atagcgataTGGATGATGAGGTTATTCAGTCCCAAGTTGGACAGAGGGATGCGGTCGTTTTTAATTTTTTTGCGGTTTGGGGGAAGGATGATATCGAGTTGGT‑GA‑T‑TTGGGAAAATATAAGCCTTTTGATTAATAACaac                                                                                                             >  2:1782105/1‑138 (MQ=255)

                             atagcgataTGGATGATGAGGTTATTCAGTCCCAAGTTGGACAGAGGGATGCGGTCGTGTTTGATTTTTTTGAGGATGGGGGAACGGGTGATCTCGATTTATT‑CA‑T‑TTTGCAAAATATCATCCTTTTAATTCAGAACAta                                                                                                             >  2:4194215/1‑139 (MQ=255)

                             atagcgataTGGATGATGAGGTTATTCAGTCCCAAGTTGGACAGAGGGATGCGGTCGTGTTTGATTTTTTTAATGATTGGGGAACGGATGATCTCGATTTGTT‑GA‑T‑TTTTGAAAAAAACAGCCTTTTAATTAAGAACaaa                                                                                                             >  2:558804/1‑138 (MQ=255)

                             atagcgataTGGATGATGAGGTTATTCAGTCCCAAGTTGGACAGAGGGATGCGGTCGTGTTTGAATTTTTTCATGATGGGGGAAAGGATGATATAGATTTCTT‑AA‑T‑TTTGAAAAAAATCATCCTTTTAAATCAGAAccac                                                                                                             >  2:3519735/1‑137 (MQ=255)

                             atagcgataTGGATGATGAGGGTATTCAGTCCCAAGTTGGACAGAGGGATGCGGTCGTTTTTGATTTTTTTGAGGATGGCGGCACGGATGATCACGCTTTATT‑AATT‑TTGGCAAAA‑ATAAGCCATTTAATTAAGAACaga                                                                                                             >  2:5133741/1‑138 (MQ=255)

                              tagcgataTGGATGATGAGGTTATTCAGTCCCAAGTTGGACAGAGGGATGCGGTCGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTT‑CA‑T‑TTGGCAAAATATCAGCCTTTTCATTCAGAACATCa                                                                                                            <  1:4161334/140‑1 (MQ=255)

                              tagcgataTGGATGATGAGGTTATTCAGTCCCAAGTTGGACAGAGGGATGCGGTCGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTT‑CA‑T‑TTGGCAAAATATCAGCCTTTTCATTCAGAACATCa                                                                                                            <  1:1500769/140‑1 (MQ=255)

                                    tatGGATGATGAGGTTATTCAGTCCCAAGTTGGACAGAGGGATGCGGTCGTGTTTCATTTTTTTGAGGATTGCGGAAACGATGATGTCGATTTATT‑AA‑T‑TTTGCAAAATATAAGCCTTTTGATTAAGAAAAACAAtatac                                                                                                      >  2:3611309/1‑136 (MQ=255)

                                          tgatgaGGTTATTCAGTCCCAAGTTGGACAGAGGGATGCGGTCGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTT‑CA‑T‑TTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTtct                                                                                                <  1:1588089/140‑1 (MQ=255)

                                          tgatgaGGTTATTCAGTCCCAAGTGGGACAGAGGGATGCGGTCGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTT‑CA‑T‑TTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTtct                                                                                                <  2:2808894/140‑1 (MQ=255)

                                                 gTTATTCAGTCCCAAGTTGGACAGAGGGATGCGGTCGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTT‑CA‑T‑TTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTTCTCTTTCAt                                                                                         <  1:205062/140‑1 (MQ=255)

                                                 gTTATTCAGTCCCAAGTTGGACAGAGGGATGCGGTCGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTT‑CA‑T‑TTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTTCTCTTTCAt                                                                                         <  1:3950845/140‑1 (MQ=255)

                                                 gTTATTCAGTCCCAAGTTGGACAGAGGGATGCGGTCGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTT‑CA‑T‑TTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTTCTCTTTCAt                                                                                         <  2:2345733/140‑1 (MQ=255)

                                                 gTTATTCAGTCCCAAGTTGGACAGAGGGATGCGGTCGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTT‑CA‑T‑TTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTTCTCTTTCAt                                                                                         <  1:5054980/140‑1 (MQ=255)

                                                   tATTCAGTCCCAAGTTGGACAGAGGGATGCGGTCGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTT‑CA‑T‑TTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTTCTCTTTCATCg                                                                                       <  1:2779504/140‑1 (MQ=255)

                                                      tCAGTCCCAAGTTGGACAGAGGGATGCGGTCGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTT‑CA‑T‑TTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTTCTCTTTCATCGACa                                                                                    <  1:1050652/140‑1 (MQ=255)

                                                      tCAGTCACAAGTTGGACAGAGGGATGCGGTCGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTT‑CA‑T‑TTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTTCTCTTTCATCGACa                                                                                    <  1:2827576/140‑1 (MQ=255)

                                                           ccccAGTTGGACAGAGGGATGCGGTCGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTT‑CA‑T‑TTGGCAAAATATCAGACTTTTCATTCAGAACATCAATCTCCGTTTCTCTTTCATCGACAATGTa                                                                               <  1:4064271/140‑1 (MQ=255)

                                                           cccAAGTTGGACAGAGGGATGCGTTCGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTT‑CA‑T‑TTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTTCTCTTTCATCGACAATGTa                                                                               <  2:131970/140‑1 (MQ=255)

                                                           cccAAGTTGGACAGAGGGATGCGGTCGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTT‑CA‑T‑TTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTTCTCTTTCATCGACAATGTa                                                                               <  2:1200650/140‑1 (MQ=255)

                                                           cccAAGTTGGACAGAGGGATGCGGTCGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTT‑CA‑T‑TTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTTCTCTTTCATCGACAATGTa                                                                               <  2:4259143/140‑1 (MQ=255)

                                                           cccAAGTTGGACAGAGGGATGCGGTCGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTT‑CA‑T‑TTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTTCTCTTTCATCGACAATGTa                                                                               <  2:3308955/140‑1 (MQ=255)

                                                           cccAAGTTGGACAGAGGGATGCGGTCGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTT‑CA‑T‑TTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTTCTCTTTCATCGACAATGTa                                                                               <  2:156441/140‑1 (MQ=255)

                                                           cccAAGTTGGACAGAGGGATGCGGTCGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTT‑CA‑T‑TTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTTCTCTTTCATCGACAATGTa                                                                               <  1:4568292/140‑1 (MQ=255)

                                                           cccAAGTTGGACAGAGGGATGCGGTCGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTT‑CA‑T‑TTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTTCTCTTTCATCGACAATGTa                                                                               <  1:4740759/140‑1 (MQ=255)

                                                           cccAAGTTGGACAGAGGGATGCGGTCGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTT‑CA‑T‑TTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTTCTCTTTCATCGACAATGTa                                                                               <  2:3124999/140‑1 (MQ=255)

                                                           cccAAGTTGGACAGAGGGATGCGGTCGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTT‑CA‑T‑TTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTTCTCTTTCATCGACAATGTa                                                                               <  2:2177931/140‑1 (MQ=255)

                                                           cccAAGTTGGACAGAGGGATGCGGTCGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTT‑CA‑T‑TTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTTCTCTTTCATCGACAATGTa                                                                               <  2:1138244/140‑1 (MQ=255)

                                                           cccAAGTTGGACAGAGGGATGCGGTCGTTTTTCATATTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTT‑CA‑T‑TTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTTCTCTTTCATCGACAATGTa                                                                               <  2:3779298/140‑1 (MQ=255)

                                                           ccaaatttGGACAGAGGGATGCGGTCGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTT‑CA‑T‑TTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTTCTCTTTCATCGACAATGTa                                                                               <  2:3259997/134‑1 (MQ=255)

                                                             cAAGTTGTACAGAGGGATGCGGTCGTTTTTCATTTTTTTCAGGATGGCGTAACGGATGATCTCTATTTATT‑CA‑T‑TTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTTCTCTTTCATCGACAATGTACt                                                                             <  2:4019334/140‑1 (MQ=255)

                                                             cAAGTTGGACAGAGGGATGCGGTCGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTT‑CA‑T‑TTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTTCTCTTTCATCGACAATGTAcg                                                                             <  1:2289389/140‑2 (MQ=255)

                                                             cAAGTTGGACAGAGGGATGCGGTCGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTT‑CA‑T‑TTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTTCTCTTTCATCGACAATGTACt                                                                             <  2:2732129/140‑1 (MQ=255)

                                                             cAAGTTGGACAGAGGGATGCGGTCGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTT‑CA‑T‑TTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTTCTCTTTCATCGACAATGTACt                                                                             <  2:1386741/140‑1 (MQ=255)

                                                                gctGGACAGAGGGATGCGGACGTTTTTCATTTTTTTCACGATGCCGGAACGGATGATCTCGATTTCGA‑CA‑T‑TTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTTCTCTTTCATCGACAATGTACTCCg                                                                          <  1:1241575/138‑1 (MQ=255)

                                                                gTTGGACAGAGGTATGCGGTCGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTT‑CA‑T‑TTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTTCTCTTTCATCGACAATGTACTCCg                                                                          <  2:2862443/140‑1 (MQ=255)

                                                                gTTGGACAGAGGGATGCGGTCGTTTTTCATTTTTTTCAGGATGGCGTAACGGATGATCTCGATTTCTT‑CA‑T‑TTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTTCTCTTTCATCGACAATGTACTCCg                                                                          <  1:3253789/140‑1 (MQ=255)

                                                                gTTGGACAGAGGGATGCGGTCGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTT‑CA‑T‑TTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTTCTCTTTCATCGACAATGTACTCCg                                                                          <  1:4304138/140‑1 (MQ=255)

                                                                gTTGGACAGAGGGATGCGGTCGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTT‑CA‑T‑TTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTTCTCTTTCATCGACAATGTACTCCg                                                                          <  1:1908830/140‑1 (MQ=255)

                                                                gTTGGACAGAGGGATGCGGTCGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTT‑CA‑T‑TTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTTCTCTTTCATCGACAATGTACTCCg                                                                          <  1:1752044/140‑1 (MQ=255)

                                                                  tgtaCAGAGGGATGCGGTCGTTTTTCATTTTTTTCATGATGGCGGAACGGATGATCTCGATTTCTT‑CA‑T‑TTGGCAAAATATCAGCCTTTTAATTCAGAACATCAATCTCCGTTTCTCTTTCATCGACAATGTACTACGcc                                                                        <  1:1841081/137‑1 (MQ=255)

                                                                  tGGACAGAGGGATGCGGTCGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTT‑CA‑T‑TTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTTCTCTTTCATCGACAATGTACTCCGcc                                                                        <  1:1187897/140‑1 (MQ=255)

                                                                  tGGACAGAGGGATGCGGTCGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTT‑CA‑T‑TTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTTCTCTTTCATCGACAATGTACTCCGcc                                                                        <  2:4392942/140‑1 (MQ=255)

                                                                   tgACAGAGGGATGCGGTCGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGAAATCGATTTCTT‑CA‑T‑TTGTCAAAATATCAGCCTTTTCATACAGAACATCAAACTCCTTTTCTCTTTCATCGACAATGTACTCCGCCa                                                                       <  1:3815928/139‑1 (MQ=255)

                                                                   ggACAGAGGGATGCGGTCGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTT‑CA‑T‑TTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTTCTCTTTCATCGACAATGTACTCCGCCa                                                                       <  1:3568150/140‑1 (MQ=255)

                                                                   ggACAGAGGGATGCGGTCGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTT‑CA‑T‑TTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTTCTCTTTCATCGACAATGTACTCCGCCa                                                                       <  1:1163996/140‑1 (MQ=255)

                                                                   ggACAGAGGGATGCGGTCGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTT‑CA‑T‑TTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTTCTCTTTCATCGACAATGTACTCCGCCa                                                                       <  1:3452130/140‑1 (MQ=255)

                                                                   ggACAGAGGGATGCGGTCGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTT‑CA‑T‑TTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTTCTCTTTCATCGACAATGTACTCCGCCa                                                                       <  1:1310928/140‑1 (MQ=255)

                                                                      cAGAGGGATGCGGTCGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTT‑CA‑T‑TTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTTCTCTTTCATCGACAATGTACTCCGCCATAc                                                                    <  2:3153371/140‑1 (MQ=255)

                                                                      cAGAGGGATGCGGTCGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTT‑CA‑T‑TTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTTCTCTTTCATCGACAATGTACTCCGCCATAc                                                                    <  2:3025631/140‑1 (MQ=255)

                                                                      cAGAGGGATGCGGTCGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTT‑CA‑T‑TTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTTCTCTTTCATCGACAATGTACTCCGCCATAc                                                                    <  1:4882479/140‑1 (MQ=255)

                                                                      cAGAGGGATGCGGTCGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTT‑CA‑T‑TTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTTCTCTTTCATCGACAATGTACTCCGCCATAc                                                                    <  1:222578/140‑1 (MQ=255)

                                                                      cAGAGGGATGCGGTCGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTT‑CA‑T‑TTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTTCTCTTTCATCGACAATGTACTCCGCCATAc                                                                    <  1:992612/140‑1 (MQ=255)

                                                                        gagGGATGCGGTCGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTT‑CA‑T‑TTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTTCTCTTTCATCGACAATGTACTCCGCCATACAg                                                                  <  1:3511935/140‑1 (MQ=255)

                                                                           ggATGCGGTCGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTT‑CA‑T‑TTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTTCTCTTTCATCGACAATGTACTCCGCCATACAGTAc                                                               <  1:921558/140‑1 (MQ=255)

                                                                           ggATGCGGTCGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGATCCCGATTTCTT‑CA‑T‑TTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTTCTCTTTCATCGACAATGTACTCCGCCATACAGTAc                                                               <  1:5133741/140‑1 (MQ=255)

                                                                                 ggTCGTTTTTCATTTTTTTCAGGCTGGCGGAACGGATGATCTCGATTTCTT‑CA‑T‑TTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTTCTCTTTCATCGACAATGTACTCCGCCATACAGTACCTCATc                                                         <  1:2531163/140‑1 (MQ=255)

                                                                                   tCGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTT‑CA‑T‑TTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTTCTCTTTCATCGACAATGTCCTCCGCCATACAGTACCTCATCTg                                                       <  1:770602/140‑1 (MQ=255)

                                                                                   tCGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTT‑CA‑T‑TTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTTCTCTTTCATCGACAATGTACTCCGCCATACAGTACCTCATCTg                                                       <  1:744214/140‑1 (MQ=255)

                                                                                        tttCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTT‑CA‑T‑TTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTTCTCTTTCATCGACAATGTACTCCGCCATACAGTACCTCATCTGCACCt                                                  <  1:2593635/140‑1 (MQ=255)

                                                                                        tttCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTT‑CA‑T‑TTGGCAAAATATCAGCCTTGTCATTCAGAACATCAATCTCCGTTTCTCTTTCATCGACAATGTACTCCGCCATACAGTACCTCATCTGCACCt                                                  <  1:1421373/140‑1 (MQ=255)

                                                                                                  ttCAGGATGGCGGAACGGATGATCTCGATTTCTT‑CA‑T‑TTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTTCTCTTTCATCGACAATGTACTCCGCCATACAGTACCTCATCTGCACCTCGTCCCcgcg                                        <  2:1917818/140‑1 (MQ=255)

                                                                                                  ttCAGGATGGCGGAACGGATGATCTCGATTTCTT‑CA‑T‑TTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTTCTCTTTCATCGACAATGTACTCCGCCATACAGTACCTCATCTGCACCTCGTCCCcgcg                                        <  2:1415363/140‑1 (MQ=255)

                                                                                                  ttCAGGATGGCGGAACGGATGATCTCGATTTCTT‑CA‑T‑TTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTTCTCTTTCATCGACAATGTACTCCGCCATACAGTACCTCATCTGCACCTCGTCCCcgcg                                        <  1:4835941/140‑1 (MQ=255)

                                                                                                  ttCAGGATGGCGGAACGGATGATCTCGATTTCTT‑CA‑T‑TTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGGTTCTCTTTCATCGACAATGTACTCCGCCATACAGTACCTCATCTGCACCTCGTCCCcgcg                                        <  2:2069930/140‑1 (MQ=255)

                                                                                                       gATGGCGGAACGGATGATCTCGATTTCTT‑CA‑T‑TTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTTCTCTTTCATCGACAATGTACTCCGCCATAc                                                                    <  1:1624033/107‑1 (MQ=255)

                                                                                                         tGGCGGAACGGATGATCTCGATTTCTT‑CA‑T‑TTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTTCTCTTTCATCGACAATGTACTCCGCCATACAGTACCTCATCTGCACCTCGTCCCCGCGCAGCTTg                                 <  1:3115382/140‑1 (MQ=255)

                                                                                                                 cGGATGATCTCGATTTCTT‑CA‑T‑TTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTTCTCTTTCATCGACAATGTACTCCGCCATACAGTACCTCATCTGCACCTCGTCCCCGCGCAGCTTGAAGCCGTa                         >  2:2218442/1‑140 (MQ=255)

                                                                                                                 cGGATGATCTCGATTTCTT‑CA‑T‑TTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTTCTCTTTCATCGACAATGTACTCCGCCATACAGTACCTCATCTGCACCTCGTCCCCGCGCAGCTAGAAGCCGTa                         >  2:4533758/1‑140 (MQ=255)

                                                                                                                    atgatCTCGATTTCTT‑CA‑T‑TTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTTCTCTTTCATCGACAATGTACTCCGCCATACAGTACCTCATCTGCACCTCGTCCCCGCGCAGCTTGAAGCCGTAAtt                      >  2:4844227/1‑140 (MQ=255)

                                                                                                                     tgatCTCGATTTCTT‑CA‑T‑TTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTTCTCTTTCATCGACAATGTACTCCGCCATACAGTACCTCATCTGCACCTCGTCCCCGCGCAGCTTGAAGCCGTAAttt                     >  1:4097448/1‑140 (MQ=255)

                                                                                                                     tgatCTCGATTTCTT‑CA‑T‑TTGGCAAAATATCAGCCTGTTCATTCAGAACATCAAGCTCGGTTTCTCTTTCATCGACATTGTACTCCGCCATACAGTACCTCATCTGCACCTCGTCCCCGCGCGGCTTGAAGCCGGAAttt                     >  1:3731194/1‑140 (MQ=255)

                                                                                                                         ctcGATTTCTT‑CA‑T‑TTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTTCTCTTTCATCGACAATGTACTCCGCCATACAGTACCTCATCTGCACCTCGTCCCCGCGCAGCTTGAAGCCGTAATTTGGGc                 <  2:742300/140‑1 (MQ=255)

                                                                                                                            gATTTCTT‑CA‑T‑TTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTTCTCTTTCATCGACAATGTACTCCGCCATACAGTACCTCATCTGCACCTCGTCCCCGCGCAGCTTGAAGCCGTAATTTGGGCgtg              <  2:4762931/140‑1 (MQ=255)

                                                                                                                             aTTTCTT‑CA‑T‑TTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTTCTCTTTCATCGACAATGTACTCCGCCATACAGTACCTCATCTGCACCTCGTCCCCGCGCAGCTTGAAGCCGTAATTTGGGCgtgt             >  1:3520872/1‑140 (MQ=255)

                                                                                                                             aTTTCTT‑CA‑T‑TTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTTCTCTTTCATCGACAATGTACTCCGCCATACAGTACCTCATCTGCACCTCGTCCCCGCGCAGCTTGAAGCCGTAATTTGGGCgtgt             <  1:3983199/140‑1 (MQ=255)

                                                                                                                             aTTTCTT‑CA‑T‑TTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTTCTCTTTCATCGACAATGTACTCCGCCATACAGTACCTCATCTGCACCTCGTCCCCGCGCAGCTTGAAGCCGTAATTTGGGCgtgt             <  1:4206554/140‑1 (MQ=255)

                                                                                                                                  tt‑cA‑T‑TTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTTCTCTTTCATCGACAATGTACTCCGCCATACAGTACCTCATCTGCACCTCGTCCCCGCGCAGCTTGAAGCCGTAATTTGGGCgt               >  1:1004714/1‑133 (MQ=255)

                                                                                                                                   t‑cA‑T‑TTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTTCTCTTTCATCGACAATGTACTCCGCCATACAGTACCTCATCTGCACCTCGTCCCCGCGCAGCTTGAAGCCGTAATTTGGGCGTGTTTCCAt       <  2:4129264/140‑1 (MQ=255)

                                                                                                                                   t‑cA‑T‑TTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTTCTCTTTCATCGACAATGTACTCCGCCATACAGTACCTCATCTGCACCTCGTCCCCGCGCAGCTTGAAGCCGTAATTTGGGCGTGTTTCCAt       <  1:620725/140‑1 (MQ=255)

                                                                                                                                   t‑cA‑T‑TTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTTCTCTTTCATCGACAATGTACTCCGCCATACAGTACCTCATCTGCACCTCGTCCCCGCGCAGCTTGAAGCCGTAATTTGGGCGTGTTTCCAt       >  2:1719130/1‑140 (MQ=255)

                                                                                                                                           tGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTTCTCTTTCATCGACAATGTACTCCGCCATACAGTACCTCATCTGCACCTCGTCCCCGCGCAGCTTGAAGCCGTAATTTGGGCGTGTTTCCATGACGa  <  1:3103781/140‑1 (MQ=255)

                                                                                                                                           tGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTTCTCTTTCATCGACAATGTACTCCGCCATACAGTACCTCATCTGCACCTCGTCCCCGCGCAGCTTGAAGCCGTAATTTGGGCGTGTTTCCATGACGa  <  1:2154554/140‑1 (MQ=255)

                                                                                                                                           tGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTTCTCTTTCATCGACAATGTACTCCGCCATACAGTACCTCATCTGCACCTCGTCCCCGCGCAGCTTGAAGCCGTAATTTGGGCGTGTTTCCATGACGa  <  1:1702698/140‑1 (MQ=255)

AGTTTTGCGTTCTGATCCGTTTACAGGCGATAGCGATATGGATGATGAGGTTATTCAGTCCCAAGTTGGACAGAGGGATGCGGTCGTTTTTCATTTTTTTCAGGATGGCGGAACGGATGATCTCGATTTCTT‑CA‑T‑TTGGCAAAATATCAGCCTTTTCATTCAGAACATCAATCTCCGTTTCTCTTTCATCGACAATGTACTCCGCCATACAGTACCTCATCTGCACCTCGTCCCCGCGCAGCTTGAAGCCGTAATTTGGGCGTGTTTCCATGACGA  >  NZ_CP034943/3790677‑3790952

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`