BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	3,851,230	G→T	6.7%	Q49K (CAG→AAG)	EO946_RS19820 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	3,851,230	0	G	T	6.7%	1048.0 / 14.2	392	Q49K (CAG→AAG)	EO946_RS19820	hypothetical protein
Reads supporting (aligned to +/- strand): ref base G (251/98); new base T (3/39); total (254/138)
Fisher's exact test for biased strand distribution p-value = 1.34e-16
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 394 total aligned reads displayed.

GTCCTGCAATGTCCCAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAA‑CGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATT  >  NZ_CP034943/3851092‑3851369

   cTGCAATGTCCCAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATc                                                                                                                                          >  1:2507112/1‑140 (MQ=255)

         tGTCCCAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGAtt                                                                                                                                    <  2:1339684/140‑1 (MQ=255)

           tCCCAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGTTATTCTATAAATGAACCAGTCGTTATTACATGTATCATGATTCg                                                                                                                                  >  2:2913208/1‑140 (MQ=255)

           tCCCAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCg                                                                                                                                  >  2:5144130/1‑140 (MQ=255)

           tCCCAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCg                                                                                                                                  >  1:1551625/1‑140 (MQ=255)

           tCCCAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCg                                                                                                                                  >  2:4041889/1‑140 (MQ=255)

           tCCCAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCg                                                                                                                                  >  2:3689874/1‑140 (MQ=255)

           tCCCAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCg                                                                                                                                  >  1:2382066/1‑140 (MQ=255)

           tCCCAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTATATAAATGAACCAGTCGTTATTAACTGTATCATGATTCg                                                                                                                                  >  2:4177069/1‑140 (MQ=255)

              cactCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATc                                                                                                                               <  1:805699/137‑1 (MQ=255)

              cAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATc                                                                                                                               <  1:3934323/140‑1 (MQ=255)

              cAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATc                                                                                                                               <  1:668836/140‑1 (MQ=255)

              cAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATc                                                                                                                               <  1:1863186/140‑1 (MQ=255)

              cAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATc                                                                                                                               <  2:2045012/140‑1 (MQ=255)

               aaTCTGCTCTCTGTAATCTGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGGAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCg                                                                                                                              <  2:4411783/140‑1 (MQ=255)

               aaTCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCCAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCg                                                                                                                              <  2:350152/140‑1 (MQ=255)

               aaTCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCg                                                                                                                              <  2:4488909/140‑1 (MQ=255)

               aaTCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCg                                                                                                                              <  2:812293/140‑1 (MQ=255)

                aTCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGt                                                                                                                             >  2:2562581/1‑140 (MQ=255)

                aTCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTAGATCGt                                                                                                                             >  2:2456382/1‑140 (MQ=255)

                    gCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGc                                                                                                                         <  2:1002091/140‑1 (MQ=255)

                     ctctctGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATTCa                                                                                                                        >  1:48632/1‑140 (MQ=255)

                     ctctctGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCa                                                                                                                        >  1:2032638/1‑140 (MQ=255)

                     ctctctGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCa                                                                                                                        >  1:3413074/1‑140 (MQ=255)

                     ctctctGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCa                                                                                                                        >  1:1450031/1‑140 (MQ=255)

                     ctctctGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCa                                                                                                                        >  2:4727446/1‑140 (MQ=255)

                     ctctctGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCa                                                                                                                        >  1:4214556/1‑140 (MQ=255)

                     ctctctGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTAGATCGTATGCa                                                                                                                        >  1:4279599/1‑140 (MQ=255)

                     ctctctGTAATATGGATTGGAAATGGAATTCTCTTGTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTCTAAATGAACCAGTCGTTATTACCTGTATCTTGAGTCGATCGTATGCa                                                                                                                        >  2:3007546/1‑140 (MQ=255)

                          tGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGccc                                                                                                                   >  1:3329612/1‑140 (MQ=255)

                          tGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGccc                                                                                                                   >  2:2861919/1‑140 (MQ=255)

                          tGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGccc                                                                                                                   <  2:1247448/140‑1 (MQ=255)

                          tGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTAACTGTATCATGATTAGATCGTATTCAATccc                                                                                                                   >  1:2927271/1‑140 (MQ=255)

                               tatGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATAATGATTCGATCGTATGCAAGCCCTTccc                                                                                                              >  2:4704327/1‑140 (MQ=255)

                                  ggATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCaaa                                                                                                           <  1:2250117/140‑1 (MQ=255)

                                  ggATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCaaa                                                                                                           <  1:443966/140‑1 (MQ=255)

                                  ggATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCaaa                                                                                                           <  1:3736090/140‑1 (MQ=255)

                                  ggATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCaaa                                                                                                           <  1:4176252/140‑1 (MQ=255)

                                    aTTGGACATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATAGTATTCAAGCCCTTCACaaaaa                                                                                                         >  1:1996392/1‑140 (MQ=255)

                                    aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATTATTCGATCGTATGCAAGCCCTTCCCaaaaa                                                                                                         >  1:3666524/1‑140 (MQ=255)

                                    aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGAtt                                                                                                                                    <  1:4563651/113‑1 (MQ=255)

                                    aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGAtt                                                                                                                                    >  1:2738588/1‑113 (MQ=255)

                                    aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCaaaaa                                                                                                         >  2:2076137/1‑140 (MQ=255)

                                    aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCaaaaa                                                                                                         >  1:5132688/1‑140 (MQ=255)

                                    aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCaaaaa                                                                                                         >  2:742006/1‑140 (MQ=255)

                                    aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCaaaaa                                                                                                         >  2:343715/1‑140 (MQ=255)

                                      tGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTGATTCCAGGTATCATTATTCGATCGTATGAAAGCCCTTCCCaaaaaag                                                                                                       >  1:610239/1‑138 (MQ=255)

                                        gAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATACATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAA‑CGAt                                                                                                    >  2:3081443/1‑140 (MQ=255)

                                                 ttCACTTTTCGCTATTTTTATCCCCTTTTAAGAATACCCTACAAAGTACTCGGACTGGTCTTCTATAAATGAACCAGTCTTTATTACCTGTATCATTATTCTATCGTATGCACCCCCTCCCCAAAAC‑CGATAACACGAct                                                                                           <  2:427321/140‑1 (MQ=255)

                                                   ctctTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAA‑CGATAAAACGActct                                                                                         >  2:704503/1‑140 (MQ=255)

                                                             tatttttATCACATTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTATATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCAAAAAA‑CGATAACACGACTCTGCTTCTTCaa                                                                               <  2:3866523/137‑1 (MQ=255)

                                                                  ttATCACCTTTTACCCATAACATTCAATGTACTCGGACAGGTATTATATAAATCACCCAGTCGTTATTACCTGTATCATGATTCGATGGTATGCACGCCCGTCCCAAAAC‑CGATAACACGACTCTGCTTCTTCAAGTTCg                                                                          <  2:4128235/140‑1 (MQ=255)

                                                                         cTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATAATGATTCGATCGTATGCAAGCCCTTCCCAAAAA‑CGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACa                                                                   >  2:4871638/1‑140 (MQ=255)

                                                                          ttGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAA‑CGATAAAACTACTCTGCTTCTTCAAGTTCGCAAGACaa                                                                  >  2:585164/1‑139 (MQ=255)

                                                                          ttGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAA‑CGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACAt                                                                  >  2:499108/1‑140 (MQ=255)

                                                                          ttGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAA‑CCATAAAACGACTCTGATACTTCAACTTCGCAAGACAt                                                                  >  1:5053321/1‑140 (MQ=255)

                                                                           tGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCACAAAAA‑CGATAAAACGACTCTTCTTCTTCAAGTTCGCAAGACaaa                                                                 >  1:1645958/1‑138 (MQ=255)

                                                                              aaGCAAAACCATCAACGAACCCGCACACGACTACAATAAAGCAACCCGACTTTATTACCTGTATCATGATACGCTCGTATCCAAGCCCTACCCAAAAA‑CGATAACACGACTCTGCTTCTTCAAGTTCGCAAGACATaaca                                                              <  2:1551625/140‑1 (MQ=255)

                                                                               aGCATAACCTTCATAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATAGTATGCAAGCCCTTCCCCAAAA‑CGATAAAACTACTCTGCTTCTTAAAGTTCGCAAGCCCTaacaa                                                             >  2:2640424/1‑140 (MQ=255)

                                                                                  aTAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAA‑CGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACtt                                                          >  1:259138/1‑140 (MQ=255)

                                                                                  aTAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAA‑CCATAAAACGACTCTGCTTATTCAAGTTCGCAAGACATAACAACtt                                                          >  1:120880/1‑140 (MQ=255)

                                                                                   tAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAA‑CGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtg                                                         >  2:33616/1‑140 (MQ=255)

                                                                                   tAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCGTCCCAAAAA‑CGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtg                                                         >  2:2155825/1‑140 (MQ=255)

                                                                                      ccTTCAACGTACTCGGACAGGTCTTATATAAATCAACCAGTCGTTATTACCTTTATCATTATTCGATCGTTTCCAAGCCCTCCCCAAAAA‑CCATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACACAACAACTtgatg                                                      <  2:3953448/140‑1 (MQ=255)

                                                                                      ccTTCAAAGTACTCGTACTGTTCTTAAATAAATTAACCAGTCTTTATTACCTGTATCATTTTTCGATCGTATGCAAGCCCTTCCCAAAAA‑CAATAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtgatg                                                      <  2:3550908/140‑1 (MQ=255)

                                                                                      ccTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAA‑CGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtgatg                                                      >  2:1424571/1‑140 (MQ=255)

                                                                                      ccTTCAAAGTACCGGGACCGGTCTTCTACAAAGTACCCCGTCGTTTTTACCTGTATCAGTTTTCGATGGTATGCAAGCCCTTCCCAAAAA‑CCATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtgatg                                                      <  2:4828202/140‑1 (MQ=255)

                                                                                      ccTACAAAGTACTCGTACAGTTCTTATAAAAATCAACCAGGGTTTATTACCTTTATAATTTTTCGATCTTCTCCACCCCCTCCCAAAAAC‑CCAAAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtgatg                                                      <  1:3787999/140‑1 (MQ=255)

                                                                                                aCTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATTCAAGCCCTTCCCAAAAA‑CGATAAAACGACTCTGCTTCTTCAATTTCGCAAGACATAACAACTTTATTAGGGTTaata                                            >  1:3954188/1‑138 (MQ=255)

                                                                                                 cTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAA‑CGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAAAAACTTGATGAGGCTTAatag                                           >  1:4365773/1‑140 (MQ=255)

                                                                                                 cTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAA‑CGATAAAAAGACTCTGCTTCTTCAAGTTGGCAATACATAACAACTTGATGAGGCTTAagag                                           >  1:528835/1‑140 (MQ=255)

                                                                                                 cTCGCACAGGTCTTCTATAAATCAACCAGTGTTAATTACCTTTAACATTTTTCGAGCTTTTGCAAGCCCTACCAAAAAA‑CTATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAagag                                           <  2:1890043/140‑1 (MQ=255)

                                                                                                    ggACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAA‑CGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGa                                        >  1:3568843/1‑140 (MQ=255)

                                                                                                     gACAGGTCTTCTATAAATGAACAAGTTGTTATTACCTGTATCATTTTTCGATGGTTTGCAAGCCCTTCCCAAAAA‑CTATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGAt                                       <  2:2480402/140‑1 (MQ=255)

                                                                                                         ggTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAA‑CGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATTAGGCTTAAGAGctgtctct                                   >  1:2573419/1‑133 (MQ=255)

                                                                                                         ggTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAA‑CGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGATGCTTAAGAGCGATAAtt                                   >  1:3120993/1‑139 (MQ=255)

                                                                                                         ggTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAA‑CGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAATAGCGATAATg                                   >  1:32010/1‑140 (MQ=255)

                                                                                                         ggTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAA‑CGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATg                                   >  1:2447441/1‑140 (MQ=255)

                                                                                                           tcttctATAAATGAACCAGTCGTTAGTACCTGTATCATGATTAGATCGTATGCCACCCCTTCCCAAAAA‑CGAAAAAACGACTCTGCATCTTCAATTTCGCAATACAAAACAACATGATGAGGCTTAAGAGCGATCAAGTc                                 >  1:1763273/1‑140 (MQ=255)

                                                                                                              tttataAATCACCCTGTGTTTTTTACGTGTAAAATGTTTCCAGCGTATCAACGCCCCACCAAAAAA‑CTAAAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTaa                              <  1:4120035/138‑1 (MQ=255)

                                                                                                                tataATTGAACCCGTCTTTATTCCCTTTATCAGTATTCGATCTTAGGCAAGCCATAACAAAAAA‑CGAAAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAAt                            <  1:1020851/140‑1 (MQ=255)

                                                                                                                 ataAATGAACCAGTCTTTATTACCTTTATCATTATGGGATCGTATGCAAGCCCTTCCCAAAAA‑CGATAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAAtt                           <  2:3587957/140‑1 (MQ=255)

                                                                                                                     aTGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAA‑CGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCg                       >  2:4566636/1‑140 (MQ=255)

                                                                                                                        cacacgTCTTTATTACGTTTATCTTTATTCCATTGTATCCAACCCTTTCCCAAAAA‑GCAAAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATa                    <  1:1269593/135‑1 (MQ=255)

                                                                                                                        accctggcGTTATTCCCTTTATCATTATTCGCGCGTATCCACGCCCTCCCAAAAAA‑CGATAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATa                    <  1:4727446/133‑1 (MQ=255)

                                                                                                                        aacacgTCTTTTTTACGTGTTTAATTATTCGATGGTCTGCAACCCCCACCCAAAAC‑CCATAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATa                    <  1:2083842/135‑1 (MQ=255)

                                                                                                                        aaCCCGTGGTTATTCCCTGTATCTGTATTCGATCGTCGGCAAGCCCTTCCAAAAAA‑CGATAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATa                    <  1:4988455/140‑1 (MQ=255)

                                                                                                                        aaCCATTCTTTATTACCTTTATCATGTTTGTATCTTA‑GCCACCCCATACCAAAAACCCAAACCACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATa                    <  1:1081272/140‑1 (MQ=255)

                                                                                                                        aaCCAGTGTTTATTACGTTTATCATTTTTCTATCGTCTGCAAGCCCTGCCAAAAAA‑CGATAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATa                    <  2:1450031/140‑1 (MQ=255)

                                                                                                                        aaCCAGTCTTTATAACCTTAATAATTATTCGATCGTATCCAACCCCTACCAAAAAA‑CCAAAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATa                    <  2:1939353/140‑1 (MQ=255)

                                                                                                                         aCCAGTCGTTATTACCTGTATCATTATTCGATCGTATGCAACCCCTTCCCAAAAA‑CGATAAAAAGACTCTGCTTCTTCAAGTTCGCAAGACAAAACAACTTTATGAGTCTTAATAGCGATCATGTCTAAATTGCCGATaa                   >  2:2248496/1‑139 (MQ=255)

                                                                                                                          ccAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAA‑CGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACt                  >  1:4790484/1‑140 (MQ=255)

                                                                                                                          ccAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAA‑CGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACt                  >  2:2777777/1‑140 (MQ=255)

                                                                                                                          ccAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAA‑CGATAAAAAGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACt                  >  1:2749280/1‑140 (MQ=255)

                                                                                                                              tCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAA‑CGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCtt              >  2:619323/1‑140 (MQ=255)

                                                                                                                              tCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAA‑CGATAAAAAGACTCTGCTTCTTCAAGGTCGCAAGACATAACAACATGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATAATGCtt              >  1:4652075/1‑140 (MQ=255)

                                                                                                                              tCGTGATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAA‑CGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGTGGCTTAATAGCGATCATGTCTAAATTTCCGATActtctt              >  1:4904330/1‑140 (MQ=255)

                                                                                                                                 ttattcCCTGTATCATGATTCGATCGTATGCACGCCCTTCCAAAAAA‑CGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTtata           <  2:3778837/140‑1 (MQ=255)

                                                                                                                                 ttattaCGTGTATATTTATTCGATCGTCTGCAAGCCCTTCCAAAAAA‑CCATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTtata           <  2:1662539/140‑1 (MQ=255)

                                                                                                                                   attaCCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAA‑CGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGc         >  2:321725/1‑140 (MQ=255)

GTCCTGCAATGTCCCAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAA‑CGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATT  >  NZ_CP034943/3851092‑3851369

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`