BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	3,851,254	G→C	7.8%	L41V (CTT→GTT)	EO946_RS19820 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	3,851,254	0	G	C	7.8%	709.4 / 8.7	353	L41V (CTT→GTT)	EO946_RS19820	hypothetical protein
Reads supporting (aligned to +/- strand): ref base G (214/97); new base C (4/29); total (227/126)
Fisher's exact test for biased strand distribution p-value = 2.26e-10
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 365 total aligned reads displayed.

CTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAA‑CGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATAAAATCTTCCCCTTATCAG  >  NZ_CP034943/3851115‑3851393

  ctGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGcc                                                                                                                                            <  1:946362/140‑1 (MQ=255)

   tGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAAGCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTGGTGAGTCCCTGTCTCATGATTCGATCGTCTGCAAGccc                                                                                                                                           >  1:3063286/1‑140 (MQ=255)

   tGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATTATTCGATCGTATTCAAGccc                                                                                                                                           >  1:2865043/1‑140 (MQ=255)

   tGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGccc                                                                                                                                           <  2:3184587/140‑1 (MQ=255)

   tGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGccc                                                                                                                                           >  1:4804700/1‑140 (MQ=255)

   tGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTAACTGTATCATGATTAGATCGTATTCAATccc                                                                                                                                           >  1:2927271/1‑140 (MQ=255)

           ggATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCaaa                                                                                                                                   <  1:4289094/140‑1 (MQ=255)

           ggATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCaaa                                                                                                                                   <  1:3736090/140‑1 (MQ=255)

             aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCaaaaa                                                                                                                                 >  2:3505073/1‑140 (MQ=255)

             aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCaaaaa                                                                                                                                 >  2:3431754/1‑140 (MQ=255)

             aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCaaaaa                                                                                                                                 >  1:4768492/1‑140 (MQ=255)

             aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCaaaaa                                                                                                                                 >  2:2076137/1‑140 (MQ=255)

             aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCGTGTAAGCATAACCTTCAAAGTACTCGGACACGTCTTCTATAAATGAACCAGTCGTTATTACCTGTAACATGATTCGATAGTATGCAAGCCCTTCCCaaaaa                                                                                                                                 >  2:2892215/1‑140 (MQ=255)

               tGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAA‑Cg                                                                                                                              >  1:722010/1‑140 (MQ=255)

               tGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAA‑Cg                                                                                                                              >  1:1393386/1‑140 (MQ=255)

                               ttttCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAA‑CGATAAAACGACTCTGct                                                                                                              >  1:4457699/1‑140 (MQ=255)

                                 ttCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATTCAAGCCCATCCCAAAAA‑CGATAAAACGACTCTGcttc                                                                                                            >  1:3587957/1‑140 (MQ=255)

                                         ttttATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAA‑CGATAAAACGACTCTGCTTCTTCAAGtt                                                                                                    >  2:727243/1‑140 (MQ=255)

                                           ttAACACCTTGAAACAATAAACTTCAAAGTACTCGCACTGTTTTTATAAAAATCAACCAGTCTTTATAACCTTTATCTTTTTTCTATCTTATGAACCCCCTACCCAAAAA‑CTATAAAACGACTCTGCTTCTTCAAGTTCg                                                                                                  <  2:518424/140‑1 (MQ=255)

                                             aTCACGTTGTAAGAATACCCTCCAAACGACTCGGACATTTTTTCAATAAATGACACAGTCTTTTTTACCGCTACCTTCATTCGATCGTATGAAAGCCCTGCCAAAAAA‑CTATAACACGACTCTGCTTCTTCAAGTTCGCa                                                                                                <  2:188531/140‑1 (MQ=255)

                                                  cTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAA‑CGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACa                                                                                           >  2:193312/1‑140 (MQ=255)

                                                   ttGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAATCCCTTCCCAAAAA‑CCATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACAt                                                                                          >  1:2272686/1‑140 (MQ=255)

                                                   ttGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAA‑CGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACaa                                                                                          >  2:3351529/1‑139 (MQ=255)

                                                   ttGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAA‑CGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACAt                                                                                          >  2:499108/1‑140 (MQ=255)

                                                    tttAATCATAACCTTAAATGTACGCGTACTGGTCTTTTTTAAATTAACAATTCTTTATTACCTTTATAATTTTTCTAGCGTATGAAAGCCCTACCCAAACA‑CCAAAACACGACTCTGCTTCTTCAAGTTCGCAAGACATa                                                                                         <  2:679178/138‑1 (MQ=255)

                                                    tGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAA‑CGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATa                                                                                         >  1:3217469/1‑140 (MQ=255)

                                                           aTAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAA‑CGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACtt                                                                                  >  1:2221442/1‑140 (MQ=255)

                                                            tAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAA‑CGATAAAACGACTCTGCTTATTCAAGTTCGCAAGACATAACAACttt                                                                                 >  1:556250/1‑139 (MQ=255)

                                                            tAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTAATAACGGTAACATGATTCGATCCTATGCAACCCCTTCCCAAAAA‑CCATAAAACGACTCTGCATCTTCAAGTTCGCAAGACATaacaacatt                                                                                 >  1:4603093/1‑137 (MQ=255)

                                                            tAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGGTATTACCTGTATAATGATTCGATCGTATGCAATCCCATCCCAAAAA‑CGAAAAAAAGACTCTGCTTCTTCAAGTTCGCAAGACATAAAAACttt                                                                                 >  2:2155883/1‑139 (MQ=255)

                                                            tAACCTTCAAAGTACACGGACAGGTCTTCTATAAATGAACCAGTCGATATTCCCTGTAACAACATTAGATAGTATGCAAGCCCTTCCCAAAAA‑AGATAAAAAGACTCTTCATCTTCAATTTAGCAAGACAaaaaaaaatt                                                                                 >  1:2071816/1‑136 (MQ=255)

                                                               tctacAAAGTACTCGGACCGGTGTTCTAAAAATCAACCAGTCTTTATTACCTGTATACTGATTCGATCGTCTCAACCCCCTGCCCAAACC‑CCAAAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtgatg                                                                              <  2:4236587/136‑1 (MQ=255)

                                                               ctttCAATTTACTCGGAATGGTTTTATATAAATTAACCCGTGGTTTTTCCCGTTATCTTTTTTCGTGCGTTTGCAACCCCGTCCCAAAAA‑CGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtgatg                                                                              <  2:1366277/138‑1 (MQ=255)

                                                               ccTTCAATTTACTGGCCCAGTTGTTCTATAAATCAACCAGTGTTTATTACCTTTATAATTATTCGAGCGTATCCAAGCCCTCCCAAAAAA‑CTAAAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtgatg                                                                              <  2:920147/140‑1 (MQ=255)

                                                               ccTTCAATGTACGCGGACCGGTGTTCTATAAATGACCCTGTGGTTATTACCTGTATCATTTTTGGAGCGTATGCAACCCCTCCCCAAAAA‑CCATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtgatg                                                                              <  2:3560685/140‑1 (MQ=255)

                                                               ccTTAAACGTCCTCGGTCCGGTCTTATATAAATGAACCAGTGGTTTTTACCTGTATAATGATACGATGGTATGCAAGCCCTACCAAAAAA‑CGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtgatg                                                                              <  2:3910895/140‑1 (MQ=255)

                                                               ccTTAAAATTACTCGGACTGTTCTTATATAAATCAACCAGTCTTTTTTACCTTTATAATGATTCGATCTTATGCAAGCCCTCCCAAAAAA‑CTATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtgatg                                                                              <  2:369278/140‑1 (MQ=255)

                                                               ccTCCAAAGTCCTCGCACAGTCGTTATATAAATCAACCAGTGTTTATTACCTGTATCATGTTTCGTTCGTATGCAAGCCCTCCCAAAAAA‑CGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtgatg                                                                              <  2:2970411/140‑1 (MQ=255)

                                                               ccTACAAAGTACGCGCACAGGTATTCTATAAATCAACCAGTCGTTTTTACCTGTATCTTTATTCGATCGTTTGCAAGCCCGGCCCAAAAA‑CGAAAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtgatg                                                                              <  2:4827512/140‑1 (MQ=255)

                                                               cattCAAATTACTGGGACAGTTCTTATATAATTGAACCATTCTTTATTACCTGTAACATTTTTCGATCGTATGCACGCCATTCCCAAAAA‑CGATAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtgatg                                                                              <  2:5060598/138‑1 (MQ=255)

                                                               cataaaaaTTACTCTGACTGTTTTTATAAAAATGAACCAGTGTTTATTACTTTTATAATGTTTCGATCTTAGGCACCCCCTCCCAAAAAA‑CTATACAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtgatg                                                                              <  1:4871638/135‑1 (MQ=255)

                                                                  tCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAA‑CGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAgg                                                                           >  1:4927787/1‑140 (MQ=255)

                                                                         aCTCGGACAGGTCTTCTATAAATGAACCAGTCGTGATTACCTGTATCATGATTCGATCGTATGCCAGCCCTTCCCAAAAA‑CGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAaga                                                                    >  1:3954347/1‑140 (MQ=255)

                                                                          cTCGGACTGGTTTTCTATAAATCAACCAGTCGTTATTCCGTGTATAATGATTCTATCGTAGGCAAGCCCTTCCAAAAAC‑CCATAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAagag                                                                   <  2:1436745/140‑1 (MQ=255)

                                                                          cTCGGACGGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCTTGATTCGATCGTATGCAAGCCCTTCCCAAAAA‑CGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACCTAACAACTTGTTGAGGCTTTagag                                                                   >  1:1993915/1‑140 (MQ=255)

                                                                          cTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAA‑CGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACCTCACAACTTGATGAGGCTTTagag                                                                   >  1:2579499/1‑140 (MQ=255)

                                                                          cTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAA‑CGATAAAACGACTCTGCTTATTCAAGTTCGCCAGACATAACAACTTGATGAGGCTTTagag                                                                   >  1:1230067/1‑140 (MQ=255)

                                                                          cTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAA‑CGATAAAAAGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAagag                                                                   >  1:3520260/1‑140 (MQ=255)

                                                                          cTCGGACAGGTCTTCTATAAATGAACCAGTCGTGATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAA‑CGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACAAAACAACTTGATGAGGCTTAagag                                                                   >  1:4700656/1‑140 (MQ=255)

                                                                           tCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAA‑CGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACAAAACAACTTGATGAGGCTTAATAGc                                                                  >  1:3953448/1‑140 (MQ=255)

                                                                           tCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTGCCCAAAAA‑CGATAAAACGACTCTGCTTATTCAAGTTCGCAAGTAATAACAACGTGATGAGGCTTAAGAGc                                                                  >  1:621868/1‑140 (MQ=255)

                                                                             ggACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAA‑CGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGa                                                                >  1:2018322/1‑140 (MQ=255)

                                                                             ggACAGGTCTTCTATAAATGAACCAGTCGAGATTACCGGGAACAAGAATCTAACGTATGCAAACCCATCCCAAAAA‑CGATAAAACGACTCAGCATCTTAAATTTCGCAACACATAACAACAATAAGACGCATAAGAGcca                                                                >  1:1262630/1‑138 (MQ=255)

                                                                              gACAGGTCTTCTATAAATGAACAAGTTGTTATTACCTGTATCATTTTTCGATGGTTTGCAAGCCCTTCCCAAAAA‑CTATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGAt                                                               <  2:2480402/140‑1 (MQ=255)

                                                                                  ggTCTTCTATAAATGAACCAGTCGTGATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAA‑CGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGCCATAACAACTTGATGAGGCTTAAGAGCGATCATg                                                           >  1:378768/1‑140 (MQ=255)

                                                                                   gtattatataAATGAACCAGTCTTTTTTACGTTTATAATGATTCGATCGTAGGCAAGCCCTGCCCAAAAA‑CGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGt                                                          <  2:1162379/134‑1 (MQ=255)

                                                                                    tcttctATAAATGAACCAGTCGTTAGTACCTGTATCATGATTAGATCGTATGCCACCCCTTCCCAAAAA‑CGAAAAAACGACTCTGCATCTTCAATTTCGCAATACAAAACAACATGATGAGGCTTAAGAGCGATCAAGTc                                                         >  1:1763273/1‑140 (MQ=255)

                                                                                       tttataAATCACCCTGTGTTTTTTACGTGTAAAATGTTTCCAGCGTATCAACGCCCCACCAAAAAA‑CTAAAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTaa                                                      <  1:4120035/138‑1 (MQ=255)

                                                                                       tatataAATCAACCAGTCTTTTTTACCTTTATCTTGATTCTATCTTCTGCAAGCCCTCCCAAAAAA‑CAAAAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTaa                                                      <  1:5144130/138‑1 (MQ=255)

                                                                                        ctATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAA‑CGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAAATTGATGATGCTTAAGATCGATCATGTCTaaa                                                     >  1:3861154/1‑140 (MQ=255)

                                                                                         tataATTGAACCCGTCTTTATTCCCTTTATCAGTATTCGATCTTAGGCAAGCCATAACAAAAAA‑CGAAAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAAt                                                    <  1:1020851/140‑1 (MQ=255)

                                                                                         tataAATTACCCTGTCTTTATTCCCTTTATATTTTTTCGATCGTATCAACGCCCTTCCAAAAAA‑CGATAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAAt                                                    <  1:4394771/140‑1 (MQ=255)

                                                                                         tataAATCAACCTGTGTTTTTTACCTTTAACATGATGCGAGCGTATGCACGCCCTCCCAAAAAC‑CCAAACCACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAAt                                                    <  1:2464782/140‑1 (MQ=255)

                                                                                             aaTGAAACAGTCCTTAATACATGTATCATGATTCGATCGTATGCAAGCCCTTGACAAAAA‑CGTTAAAACTCATCTGCTTATTCAATTTCGCAATAAAAAACAACTATATGAGGCTTAACAGCGAGCAAGGGTAAAtagac                                                >  2:272395/1‑136 (MQ=255)

                                                                                                 aaCCATTCTTTATTACCTTTATCATGTTTGTATCTTA‑GCCACCCCATACCAAAAACCCAAACCACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATa                                            <  1:1081272/140‑1 (MQ=255)

                                                                                                 aaCCAGTCGTTATTACCTGTATCATTATTCGAGCGTATGCACCCCCTTCCCAAAAA‑CGATAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATa                                            <  2:4736374/140‑1 (MQ=255)

                                                                                                  aCCAGTCGTTATTACCTGTATCATTATTCGATCGTATGCAACCCCTTCCCAAAAA‑CGATAAAAAGACTCTGCTTCTTCAAGTTCGCAAGACAAAACAACTTTATGAGTCTTAATAGCGATCATGTCTAAATTGCCGATaa                                           >  2:2248496/1‑139 (MQ=255)

                                                                                                   ccAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAA‑CGATAAAAAGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACt                                          >  1:2749280/1‑140 (MQ=255)

                                                                                                     aGTCTTTATTACCTGTATCATGATTAGATCGTATGCAAGCCCTTCCCAAAAA‑CGATAAAACGACTCTGCTTCTTCAAGTTCTCAAGACATAACAACTTGATGAGGCTTAAGATCGATAATGTCTCAATTGACGAAACAGc                                        >  2:4503150/1‑140 (MQ=255)

                                                                                                          ttattcCCTGTATCATGATTCGATCGTATGCACGCCCTTCCAAAAAA‑CGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTtata                                   <  2:3778837/140‑1 (MQ=255)

                                                                                                              taCCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAA‑CGATCAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGAGCCTGTCTAAATTGCCGATACTGCTTATAGCgg                               >  2:60994/1‑140 (MQ=255)

                                                                                                                    tATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAA‑CGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTAttt                         >  1:4200703/1‑140 (MQ=255)

                                                                                                                    tATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAA‑CGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTAttt                         >  1:2661215/1‑140 (MQ=255)

                                                                                                                    tATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAA‑CGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTAttt                         >  2:786020/1‑140 (MQ=255)

                                                                                                                     aTCATGATTAGATCGTATGCAAGCCCTTCCAAAAAA‑CGATAAAACGACTCTTCTTCTTCAAGTTCGCAAGACATAACAAATTGATGAGGATTAAGAGCGATCATGTCTAAATTGACGATACTGCTTATAGCGGTTAttta                        >  2:4445889/1‑139 (MQ=255)

                                                                                                                         ttttccGATGGTATCCAACCCCTCCCCAAAAA‑CCAAAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATa                    <  1:254935/135‑1 (MQ=255)

                                                                                                                         ttaTTCGATCGTAGGAAAGCCCTTCCAAAAAA‑CTATAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATa                    <  2:1514825/138‑1 (MQ=255)

                                                                                                                              ctaTCTTATGAAACCCCTTCAAAAAAA‑CTAAAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATAAAATc               <  2:3954188/138‑1 (MQ=255)

                                                                                                                              cgctCGTATCCACCCCCTTCCCAAAAA‑CTAAAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATAAAATc               <  2:1830368/137‑1 (MQ=255)

                                                                                                                              cGATCGTATGCAAGCCCTTCCCAAAAA‑CGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATAAAATc               >  1:1652840/1‑140 (MQ=255)

                                                                                                                              cGATCGTATGCAAGCCCTTCCCAAAAA‑CGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATAAAATc               >  1:160809/1‑140 (MQ=255)

                                                                                                                              cGATCGTATGCAAGCCCTTCCCAAAAA‑CGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATAAAATc               >  1:344580/1‑140 (MQ=255)

                                                                                                                                aTCGTATGCAAGCCCTTCCCAAAAA‑CGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATAAAATcgt             >  2:567249/1‑138 (MQ=255)

                                                                                                                                 tcttttGCAAGCCCTTCCCAAAAA‑CTATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATAAAATCTTc            <  2:2447441/135‑1 (MQ=255)

                                                                                                                                   ttATGCAACCCCTTCCCAAAAA‑CCATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATAAAATCTTccc          <  2:2529703/139‑1 (MQ=255)

                                                                                                                                   ctATGCACGCCCTTCCCCAAAA‑CGATAAAACGACTCTGCTTGTTCCAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTCCCGATACTGCTTATAGCGGTTATTTCCATAAAATCGTccc          <  2:2806586/139‑1 (MQ=255)

                                                                                                                                    tATGCAAGCCCTTCCCAAAAA‑CGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATAAAATCTTcccc         >  1:3150941/1‑140 (MQ=255)

                                                                                                                                     aTGCAAGCCCTTCCCAAAAA‑CGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATAAAATCTTCCCCt        >  1:3189646/1‑140 (MQ=255)

                                                                                                                                      tcaacgCCCTACCCAAAAA‑CTATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATAAAATCTTCCCCtt       <  1:3351529/135‑1 (MQ=255)

                                                                                                                                      tGCAAGCCCTTCCCAAAAA‑CGATAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATAAAATCTTCCCCtt       <  2:1465019/140‑1 (MQ=255)

                                                                                                                                      tGCAAGCCCTTCCCAAAAA‑CGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATAAAATCTTCCCCtt       >  2:4159220/1‑140 (MQ=255)

                                                                                                                                      tGCAAGCCCTTCAAAAAAA‑CTATAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATAAAATCTTCCCCtt       <  2:461557/140‑1 (MQ=255)

                                                                                                                                      gccAAGCCCTGCCCAAAAA‑CGAAAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATAAAATCTTCCCCtt       <  1:499108/138‑1 (MQ=255)

                                                                                                                                        cacgCCCGTCCCAAAAC‑CGATAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATAAAATCTTCCCCTTAt     <  2:3063286/137‑1 (MQ=255)

                                                                                                                                        cAAGCCGTTCCAAAAAC‑CGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATAAAATCTTCCCCTTAt     <  2:4109501/140‑1 (MQ=255)

                                                                                                                                        cAAGCCCTTCCCAAAAA‑CTATAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATAAAATCTTCCCCTTAt     <  2:2032638/140‑1 (MQ=255)

                                                                                                                                        cAAGCCCTTCCAAAAAC‑CTAAAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATAAAATCTTCCCCTTAt     <  2:1609062/140‑1 (MQ=255)

                                                                                                                                        cAAGCCCTCCACAAAAA‑CTATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATAAAATCTTCCCCTTAt     <  2:48632/140‑1 (MQ=255)

                                                                                                                                           gCCCTTCCCAAAAA‑CGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATAAAATCTTCCCCTTATCAg  >  2:4027881/1‑140 (MQ=255)

                                                                                                                                           gCCCTACCAAAAAA‑CTATAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATAAAATCTTCCCCTTATCAg  <  1:5108177/140‑1 (MQ=255)

CTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAA‑CGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATAAAATCTTCCCCTTATCAG  >  NZ_CP034943/3851115‑3851393

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`