BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	3,851,262	C→A	18.2%	W38L (TGG→TTG)	EO946_RS19820 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	3,851,262	0	C	A	18.2%	771.0 / 60.5	366	W38L (TGG→TTG)	EO946_RS19820	hypothetical protein
Reads supporting (aligned to +/- strand): ref base C (222/66); new base A (2/76); total (224/142)
Fisher's exact test for biased strand distribution p-value = 5.86e-36
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 374 total aligned reads displayed.

TATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAA‑GCCCTTCCCAAAAA‑CGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATAAAATCTTCCCCTTATCAGTTGGAT  >  NZ_CP034943/3851123‑3851399

   ggATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAA‑GCCCTTCCCaaa                                                                                                                                         <  1:1559283/140‑1 (MQ=255)

   ggATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAA‑GCCCTTCCCaaa                                                                                                                                         <  1:4176252/140‑1 (MQ=255)

     atacaaaaTGAAATTCTAATTTCGCTAGTTTTATCAAATTGTAAGAATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCTTTATTAAATTTATAATTATTCAATCGTATTAAA‑ACCCATCCCaaaaa                                                                                                                                       >  1:1386481/6‑140 (MQ=255)

     aTTGGACATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATAGTATTCAA‑GCCCTTCACaaaaa                                                                                                                                       >  1:1996392/1‑140 (MQ=255)

     aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAATCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTAGTTATTACCTGTATCATGATTCGATCGTATGCAA‑GCCCTTCCCaaaaa                                                                                                                                       >  2:1556155/1‑140 (MQ=255)

     aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCCA‑GCCCTTCCCcaaaa                                                                                                                                       >  1:4492998/1‑140 (MQ=255)

     aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAA‑GCCCTTCCCaaaaa                                                                                                                                       >  2:2076137/1‑140 (MQ=255)

     aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAA‑GCCCTTCCCaaaaa                                                                                                                                       >  1:4311467/1‑140 (MQ=255)

     aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAA‑GCCCTTCCCaaaaa                                                                                                                                       >  1:4768492/1‑140 (MQ=255)

     aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAA‑GCCCTTCCCaaaaa                                                                                                                                       >  2:343715/1‑140 (MQ=255)

     aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAA‑GCCCTTCCCaaaaa                                                                                                                                       >  1:3806954/1‑140 (MQ=255)

     aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAA‑GCCCTTCCCaaaaa                                                                                                                                       >  1:5132688/1‑140 (MQ=255)

     aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAA‑GCCCTTCCCaaaaa                                                                                                                                       >  1:3704535/1‑140 (MQ=255)

     aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAA‑GCCCTTCCCaaaaa                                                                                                                                       >  1:2549054/1‑140 (MQ=255)

     aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCGTGTAAGCATAACCTTCAAAGTACTCGGACACGTCTTCTATAAATGAACCAGTCGTTATTACCTGTAACATGATTCGATAGTATGCAA‑GCCCTTCCCaaaaa                                                                                                                                       >  2:2892215/1‑140 (MQ=255)

       tGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAA‑GCCCTTCCCAAAAA‑Cg                                                                                                                                    >  1:4408169/1‑140 (MQ=255)

       tGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAA‑GCCCTTACCAAAAA‑Cg                                                                                                                                    >  1:1805728/1‑140 (MQ=255)

                  ttCACTTTTCGCTATTTTTATCCCCTTTTAAGAATACCCTACAAAGTACTCGGACTGGTCTTCTATAAATGAACCAGTCTTTATTACCTGTATCATTATTCTATCGTATGCAC‑CCCCTCCCCAAAAC‑CGATAACACGAct                                                                                                                         <  2:427321/140‑1 (MQ=255)

                    ctctTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAA‑GCCCTTCCCAAAAA‑CGATAAAACGActct                                                                                                                       >  2:2799277/1‑140 (MQ=255)

                                          cTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAA‑GCCCTTCCCAAAAA‑CGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACa                                                                                                 >  2:3787999/1‑140 (MQ=255)

                                           ttGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAA‑GCCCTTCCCAAAAA‑CGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACAt                                                                                                >  2:499108/1‑140 (MQ=255)

                                            tttacgaaTAACCTTAAAAGTACTCGTACTGGTCTTCTATAAATGAACCAGTCTTTTTTACCTTTATATTTTTTCTATCGTATGCAC‑GCCCTTCCAAAAAA‑CGATAACACGACTCTGCTTCTTCAAGTTCGCAAGACATa                                                                                               <  2:1505626/133‑1 (MQ=255)

                                            tttAATCATAACCTTAAATGTACGCGTACTGGTCTTTTTTAAATTAACAATTCTTTATTACCTTTATAATTTTTCTAGCGTATGAAA‑GCCCTACCCAAACA‑CCAAAACACGACTCTGCTTCTTCAAGTTCGCAAGACATa                                                                                               <  2:679178/138‑1 (MQ=255)

                                            tGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAA‑GCCCTTCCCAAAAA‑CGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATa                                                                                               >  1:3217469/1‑140 (MQ=255)

                                            tGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAA‑GCCCTTCACAAAAA‑CGATAAAACGACTCTTCTTCTTCAAGTTCGCAAGACaaa                                                                                               >  1:1645958/1‑138 (MQ=255)

                                                    tAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTCCCTGTATCATGATTCGATCGTAAGCAA‑GCCCTTCCCAAAAA‑CTATAAAAAGAATCTGCTTCTTCAATTTCGCAAGACATAACAAAttt                                                                                       >  1:576677/1‑139 (MQ=255)

                                                    tAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAA‑GCCCTTCCCAAAAA‑CGATCAAACGACTCTGCTTCTTCAAGTTCGCAATCCATAACAACTtg                                                                                       >  2:719978/1‑140 (MQ=255)

                                                    tAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAA‑GCCCTTCCCAAAAA‑CGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtg                                                                                       >  2:33616/1‑140 (MQ=255)

                                                    tAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAA‑GCCCTTCCCAAAAA‑CGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAAAAACTtg                                                                                       >  2:3027327/1‑140 (MQ=255)

                                                    tAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAA‑GCCCTTCCCAAAAA‑CGATAAAACGACACTGCTTCTTAAAGTTAGCAAGACATAAAAAATtg                                                                                       >  1:210279/1‑140 (MQ=255)

                                                    tAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAA‑GCCCGTCCCAAAAA‑CGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtg                                                                                       >  2:2155825/1‑140 (MQ=255)

                                                       cttacAAATTCCTCGCACTGTTCTTATATAAAGCAACCAGTTGTTATTCCCTTTATATTTATTCTATGTTAGCCAA‑GCACTTCCAAAAAA‑CCAAAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtgatg                                                                                    <  1:2909511/136‑1 (MQ=255)

                                                       ccTTCAATTTACTGGCCCAGTTGTTCTATAAATCAACCAGTGTTTATTACCTTTATAATTATTCGAGCGTATCCAA‑GCCCTCCCAAAAAA‑CTAAAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtgatg                                                                                    <  2:920147/140‑1 (MQ=255)

                                                       ccTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAA‑GCCCTTCCCAAAAA‑CGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtgatg                                                                                    >  2:1424571/1‑140 (MQ=255)

                                                       ccTTAAACGTCCTCGGTCCGGTCTTATATAAATGAACCAGTGGTTTTTACCTGTATAATGATACGATGGTATGCAA‑GCCCTACCAAAAAA‑CGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtgatg                                                                                    <  2:3910895/140‑1 (MQ=255)

                                                       ccTTAAAATTACTCGGACTGTTCTTATATAAATCAACCAGTCTTTTTTACCTTTATAATGATTCGATCTTATGCAA‑GCCCTCCCAAAAAA‑CTATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtgatg                                                                                    <  2:369278/140‑1 (MQ=255)

                                                       ccTACAAAGTACTCGTACAGTTCTTCTAAAAATCAACCATAGCTTATTACATGTATCATGATTCGATCGTATGCAC‑CCCCTTCCCAAAAC‑CCAAAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtgatg                                                                                    <  2:194853/140‑1 (MQ=255)

                                                       ccTACAAAGTACGCGCACAGGTATTCTATAAATCAACCAGTCGTTTTTACCTGTATCTTTATTCGATCGTTTGCAA‑GCCCGGCCCAAAAA‑CGAAAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtgatg                                                                                    <  2:4827512/140‑1 (MQ=255)

                                                       cattCAAATTACTGGGACAGTTCTTATATAATTGAACCATTCTTTATTACCTGTAACATTTTTCGATCGTATGCAC‑GCCATTCCCAAAAA‑CGATAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtgatg                                                                                    <  2:5060598/138‑1 (MQ=255)

                                                            aaaGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAA‑GCCCGTCCCAAAAA‑CGATAAAACGACGCTGCTTCTTCAAGTTCGCAAGAAATAACAACATGATTAGGCt                                                                               >  1:461557/1‑140 (MQ=255)

                                                                  cTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAA‑GCCCTTCCCAAAAA‑CGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACCTCACAACTTGATGAGGCTTTagag                                                                         >  1:2579499/1‑140 (MQ=255)

                                                                  cTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAA‑GCCCTTCCCAAAAA‑CGATAAAACGACTCTGCTTCTTCAAGGTCGCAAGACATAACAACTTGATGAGGCTTAagag                                                                         >  1:4826565/1‑140 (MQ=255)

                                                                  cTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAA‑GCCCTTCCCAAAAA‑CGATAAAACGACTCTGCTTATTCAAGTTCGCCAGACATAACAACTTGATGAGGCTTTagag                                                                         >  1:1230067/1‑140 (MQ=255)

                                                                  cTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAA‑GCCCTTCCCAAAAA‑CGATAAAAAGACTCTGCTTCTTCAAGTTGGCAATACATAACAACTTGATGAGGCTTAagag                                                                         >  1:528835/1‑140 (MQ=255)

                                                                  cTCGGACAGGTCTTCTATAAATGAACCAGTCGTGATTACCTGTATCATGATTCGATCGTATGCAA‑GCCCTTCCCAAAAA‑CGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACAAAACAACTTGATGAGGCTTAagag                                                                         >  1:4700656/1‑140 (MQ=255)

                                                                   tCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAA‑GCCCTTCCCAAAAA‑CGATAAAACGACTCTGCTTCTTCCAGTTCGCAAGACATAACAACTTGATGAGGCTTAATAGc                                                                        >  1:4236587/1‑140 (MQ=255)

                                                                   tCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAA‑GCCCGTCCCAAAAA‑CGATAAAAAGACTCTGCTTCTTCAAGTTCGCAAGACAGAACAACTTTATGAGGCTTAAGAGc                                                                        >  1:3953749/1‑140 (MQ=255)

                                                                     ggACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAA‑GCCCTTCCCAAAAA‑CGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACATGATGAGGCTTAAGAGCGa                                                                      >  1:3574131/1‑140 (MQ=255)

                                                                      gACAGGTCTTCTATAAATGAACAAGTTGTTATTACCTGTATCATTTTTCGATGGTTTGCAA‑GCCCTTCCCAAAAA‑CTATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGAt                                                                     <  2:2480402/140‑1 (MQ=255)

                                                                      caCAGGTATTAAATAAATGAACCTGTCGTTATTACGTGTTTCATTTTTCGATCTCATGCAA‑GCCCTCCCCAAAAA‑CTATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGAt                                                                     <  2:5161962/139‑1 (MQ=255)

                                                                          ggTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAA‑GCCCTTCCCAAAAA‑CGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATg                                                                 >  1:2447441/1‑140 (MQ=255)

                                                                          ggTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAA‑GCCCTTCCCAAAAA‑CGATAAAACGACTCTGCTTATTAAAGTTAGCAAGACATAACAAATTGATGAGGCTTAATAGCGATCATg                                                                 >  1:3921415/1‑140 (MQ=255)

                                                                           gTCTTATAAAAATGAACCCGTCGTTTTTACCTGTATAATTATTCGAGCGTATGCAA‑CCCCGCCCCAAAAA‑CTATAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGt                                                                <  2:4417934/140‑1 (MQ=255)

                                                                               tataaaaaTTAACCCGACGTTATTACCTGAAACATCATTCCATCGTCTGAAA‑CCCCTACAAAAAAA‑CTAAAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTaa                                                            <  1:3745685/135‑1 (MQ=255)

                                                                                 tataATTGAACCCGTCTTTATTCCCTTTATCAGTATTCGATCTTAGGCAA‑GCCATAACAAAAAA‑CGAAAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAAt                                                          <  1:1020851/140‑1 (MQ=255)

                                                                                  ataAATGAACCAGTCTTTATTACCTTTATCATTATGGGATCGTATGCAA‑GCCCTTCCCAAAAA‑CGATAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAAtt                                                         <  2:3587957/140‑1 (MQ=255)

                                                                                         cacacgTCTTTATTACGTTTATCTTTATTCCATTGTATCCAA‑CCCTTTCCCAAAAA‑GCAAAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATa                                                  <  1:1269593/135‑1 (MQ=255)

                                                                                         aaCCCGTGGTTTTTACCTGTATAATGATTCGATCGTA‑GCAACGCCCTACCAAAAAA‑CCAAAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATa                                                  <  1:4988370/140‑1 (MQ=255)

                                                                                         aaCCATTCTTTATTACCTTTATCATGTTTGTATCTTA‑GCCA‑CCCCATACCAAAAACCCAAACCACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATa                                                  <  1:1081272/140‑1 (MQ=255)

                                                                                         aaCCAGTGTTTATTACGTTTATCATTTTTCTATCGTCTGCAA‑GCCCTGCCAAAAAA‑CGATAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATa                                                  <  2:1450031/140‑1 (MQ=255)

                                                                                         aaCCAGTCGTTATTACCTGTATCATTATTCGAGCGTATGCAC‑CCCCTTCCCAAAAA‑CGATAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATa                                                  <  2:4736374/140‑1 (MQ=255)

                                                                                          aCCAGTCGTTATTACCTGTATCATTATTCGATCGTATGCAA‑CCCCTTCCCAAAAA‑CGATAAAAAGACTCTGCTTCTTCAAGTTCGCAAGACAAAACAACTTTATGAGTCTTAATAGCGATCATGTCTAAATTGCCGATaa                                                 >  2:2248496/1‑139 (MQ=255)

                                                                                           ccAGTCGTTTTTAACTGTATCATTTTTCGAGCGTATGCAC‑CCCCTTCCAAAAAA‑CTATAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACt                                                <  2:2927271/140‑1 (MQ=255)

                                                                                           ccAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAA‑GCCCTTCCCAAAAA‑CGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAAAAACTTGATGAGGCGTAAGAGCGATCATTTCTAAATTGCCGATACt                                                >  1:78540/1‑140 (MQ=255)

                                                                                           ccAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAA‑GCCCTTCCCAAAAA‑CGATAAAAAGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACt                                                >  1:2749280/1‑140 (MQ=255)

                                                                                             aGTCTTTATTACCTGTATCATGATTAGATCGTATGCAA‑GCCCTTCCCAAAAA‑CGATAAAACGACTCTGCTTCTTCAAGTTCTCAAGACATAACAACTTGATGAGGCTTAAGATCGATAATGTCTCAATTGACGAAACAGc                                              >  2:4503150/1‑140 (MQ=255)

                                                                                               tCGTTATTACCTGTATCATGATTCGATCGTATGCAA‑GCCCTTCCCAAAAA‑CGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACCTAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCtt                                            >  1:3357019/1‑140 (MQ=255)

                                                                                               tCGTTATTACCTGTATCATGATTCGATCGTATGCAA‑GCCCTTCCCAAAAA‑CGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCtt                                            >  2:4162851/1‑140 (MQ=255)

                                                                                               tCGTTATTACCTGTATCATGATTCGATCGTATGCAA‑GCCCTTCCCAAAAA‑CCATAAAAAGACTCTGCTTATTCAAGTTCGCAAGACATAACAACATGATGAGGCTTAATAGCGATAATGTCTAAATTGCCGATACTGCtt                                            >  1:1686161/1‑140 (MQ=255)

                                                                                               tCGTGATTACCTGTATCATGATTCGATCGTATGCAA‑GCCCTTCCCAAAAA‑CGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGTGGCTTAATAGCGATCATGTCTAAATTTCCGATActtctt                                            >  1:4904330/1‑140 (MQ=255)

                                                                                                  ttattaCGTTAATAATGTTTCGACCGCAGGCAA‑GCCCGACCAAAAAC‑CAAAACAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTtata                                         <  1:661369/140‑1 (MQ=255)

                                                                                                  ttattaCGTGTATATTTATTCGATCGTCTGCAA‑GCCCTTCCAAAAAA‑CCATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTtata                                         <  2:1662539/140‑1 (MQ=255)

                                                                                                    attaCCTGTATCATGATTCGATCGTATGCAA‑GCCCTTCCCAAAAA‑CGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGc                                       >  2:321725/1‑140 (MQ=255)

                                                                                                      taCCTGTATCATGATTCGATCGTATGCAA‑GCCCTTCCCAAAAA‑CGATCAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGAGCCTGTCTAAATTGCCGATACTGCTTATAGCgg                                     >  2:60994/1‑140 (MQ=255)

                                                                                                            tATCATGATTCGATCGTATGCAA‑GCCCTTCCCAAAAA‑CGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATATCGGTTAttt                               >  1:5028383/1‑140 (MQ=255)

                                                                                                            tATCATGATTCGATCGTATGCAA‑GCCCTTCCCAAAAA‑CGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTAttt                               >  2:786020/1‑140 (MQ=255)

                                                                                                            tATCATGATTCGATCGTATGCAA‑GCCCTTCCCAAAAA‑CGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTAttt                               >  1:3186664/1‑140 (MQ=255)

                                                                                                            tATCATGATTCGATCGTATGCAA‑GCCCTTCCCAAAAA‑CGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCAGGTCTAAATTGCCGATACTGCTTATAGCGGTTAttt                               >  1:2222140/1‑140 (MQ=255)

                                                                                                            tATCATGATTCGATCGTATGCAA‑GCCCTTCCAAAAAA‑CGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGATTATAGCGGTTAttt                               >  1:353127/1‑140 (MQ=255)

                                                                                                                   aTTCGATCGTATGCAA‑GCCCTTCCCAAAAA‑CGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATaaa                        >  1:2055683/1‑140 (MQ=255)

                                                                                                                      ctaTCTTATGAAA‑CCCCTTCAAAAAAA‑CTAAAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATAAAATc                     <  2:3954188/138‑1 (MQ=255)

                                                                                                                      cGATCGTATGCAA‑GCCCTTCCCAAAAA‑CGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATAAAATc                     >  1:3049980/1‑140 (MQ=255)

                                                                                                                      cGATCGTATGCAA‑GCCCTTCCCAAAAA‑CGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATAAAATc                     >  1:344580/1‑140 (MQ=255)

                                                                                                                        aTCGTATGCAA‑GCCCTTCCCAAAAA‑CGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATAAAATCtt                   >  2:1864101/1‑140 (MQ=255)

                                                                                                                         tcttttGCAA‑GCCCTTCCCAAAAA‑CTATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATAAAATCTTc                  <  2:2447441/135‑1 (MQ=255)

                                                                                                                           ttATGCAA‑CCCCTTCCCAAAAA‑CCATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATAAAATCTTccc                <  2:2529703/139‑1 (MQ=255)

                                                                                                                            tATGCAA‑GCCCTTCCCAAAAA‑CGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATAAAATCTTcccc               >  1:3150941/1‑140 (MQ=255)

                                                                                                                             aTGCAA‑GCCCTTCCCAAAAA‑CGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATAAAATCTTCCCCt              >  1:2645827/1‑140 (MQ=255)

                                                                                                                              tGCAA‑GCCCTTCCAAAAAA‑CGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATAAAATCTTCCCCtt             <  2:1105616/140‑1 (MQ=255)

                                                                                                                              tGCAA‑GCCCTTCAAAAAAA‑CTATAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATAAAATCTTCCCCtt             <  2:461557/140‑1 (MQ=255)

                                                                                                                                cAA‑GCCGTTCCAAAAAC‑CGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATAAAATCTTCCCCTTAt           <  2:4109501/140‑1 (MQ=255)

                                                                                                                                cAA‑GCCCTCCACAAAAA‑CTATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATAAAATCTTCCCCTTAt           <  2:48632/140‑1 (MQ=255)

                                                                                                                                    gCCCTTCCCAAAAA‑CGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATAAAATCTTCCCCTTATCAg        >  2:4027881/1‑140 (MQ=255)

                                                                                                                                    gCCCTTCCCAAAAA‑CGAAAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTGGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATAAAATCTTCCCCTTATCAg        <  2:1906095/140‑1 (MQ=255)

                                                                                                                                    gCCCTTCCAAAAAC‑CGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATAAAATCTTCCCCTTATCAg        <  2:556250/140‑1 (MQ=255)

                                                                                                                                    ccccTTCCAAAAAA‑CTATACAACGACTCTGCTTCTTCAACTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATAAAATCTTCCCCTTATCAg        <  1:4041889/139‑1 (MQ=255)

                                                                                                                                       cTTCCCAAAAA‑CGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATAAAATCTTCCCCTTATCAGTTg     >  2:1357138/1‑140 (MQ=255)

                                                                                                                                        ttCCCAAAAA‑CGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATAAAATCTTCCCCTTATCAGtttg    >  2:204678/1‑138 (MQ=255)

                                                                                                                                        ttCCCAAAAA‑CGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATAAAATCTTCCCCTTATCAGTTgg    >  2:2718956/1‑140 (MQ=255)

                                                                                                                                          cccAAAAA‑CGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATAAAATCTTCCCCTTATCAGTTGGAt  >  2:3523488/1‑140 (MQ=255)

TATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAA‑GCCCTTCCCAAAAA‑CGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATAAAATCTTCCCCTTATCAGTTGGAT  >  NZ_CP034943/3851123‑3851399

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`