BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	2,991,252	C→A	26.2%	intergenic (+53/‑154)	gbsR → / → bslA	transcriptional regulator GbsR/biofilm‑surface layer protein BslA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	2,991,252	0	C	A	26.2%	199.6 / 25.4	127	intergenic (+53/‑154)	gbsR/bslA	transcriptional regulator GbsR/biofilm‑surface layer protein BslA
Reads supporting (aligned to +/- strand): ref base C (26/60); new base A (36/2); total (65/62)
Fisher's exact test for biased strand distribution p-value = 6.83e-12
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 127 total aligned reads displayed.

TACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTAC‑AAATTTGAAAAATAC‑GGGAAAAAC‑AT‑CCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCGGGCAGGCCTGAATATTTTTATAACT  >  NZ_CP034943/2991119‑2991386

tACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGTTTTTTTTTTTTTTTTTTTTATTAATAC‑aaa                                                                                                                                     >  1:65148/1‑140 (MQ=255)

tACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGTTTTTTTTTTTTTTTTTTTAATTAATAa‑aaa                                                                                                                                     >  1:281973/1‑140 (MQ=255)

tACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGTTTTTTTTGTTTTTTTTGTTATTAATAC‑aaa                                                                                                                                     >  1:3253949/1‑140 (MQ=255)

 aCTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGTTTTTTTTTTTTTTTTTTTTATTAATAa‑aaaa                                                                                                                                    >  1:669603/1‑139 (MQ=255)

 aCTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGTTTTTTTTGTTTTTTTTTTTATTAATAA‑AAAt                                                                                                                                    >  1:3630461/1‑140 (MQ=255)

 aCTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGGTTTTGTTGTTTTTTTTTTAAATAATAC‑aaaa                                                                                                                                    >  1:1265163/1‑139 (MQ=255)

        ttGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTTTTGTTTTTTATTTAATTAAAAA‑AAAATTTaaaa                                                                                                                             >  1:518108/1‑140 (MQ=255)

        ttGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTGTTTTTTTTTTAATTACAAA‑AAAATTGtaaa                                                                                                                             >  1:101594/1‑140 (MQ=255)

           gCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTTAAACAAAGTAAAACAAAAACGCCTGGGAAACAGGGCGTTTTTTTTTTCTTTTTTATTTAATTAATAA‑AAATTTTAAAAATa                                                                                                                          >  2:306359/1‑140 (MQ=255)

           gCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTTTTCTTTTTTTTTTAATTACTAC‑AAATTTTTAAAATa                                                                                                                          >  2:1061787/1‑140 (MQ=255)

           gCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTAC‑AAAATTTaaaaaaa                                                                                                                          >  2:306384/1‑138 (MQ=255)

           gCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGTTTTTTTTCGTGTGTTTGTGATTAATAA‑CAATTTGTAAAAtg                                                                                                                          >  1:1987969/1‑139 (MQ=255)

           gCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGTTTTTGTTGTTTTTTTTTTTATTTATAA‑AAAATTTTAAAATa                                                                                                                          >  1:1403928/1‑140 (MQ=255)

           gCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGGTTTTTTTGTTTTTTATTTAAATAAAAC‑AAAATTGAAAAATa                                                                                                                          >  1:1406380/1‑140 (MQ=255)

            cTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTTTTGTTTTTTATTTAATTACAAA‑AAATTTTAAAAAAAc                                                                                                                         >  2:468634/1‑140 (MQ=255)

             tGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTTTTGTTTTTTATGTAATTAATAC‑AAAATTGAAAAATAC‑g                                                                                                                       >  2:1187630/1‑140 (MQ=255)

             tGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTAATAC‑AAATTTTAAAAATAC‑g                                                                                                                       >  2:923328/1‑140 (MQ=255)

             tGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTGTTTGGTTGTTTTTTATGTTATTAATAC‑AAATTTGAAAAATAC‑g                                                                                                                       >  2:2370982/1‑140 (MQ=255)

             tGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGTTTTTTTTGTTTTTTATTTAATTAATAA‑ACATTTGTAAAATaag                                                                                                                        >  2:266532/1‑138 (MQ=255)

             tGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGTTTTTTTTATTTTTTATTTAAATAATAC‑AAATTTTaaaaaaaac                                                                                                                        >  2:6515/1‑136 (MQ=255)

             tGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGTTTTTGTTTTTTTTTATTTAATTAATAA‑AAATTTGTAAAATaag                                                                                                                        >  2:2316996/1‑138 (MQ=255)

             tGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAAAGCCTGGGAAACCGGGCGTTTGTTTGTTTTTGTTTTTGTAATTAAAAC‑AAATTTTaacaaaaag                                                                                                                        >  2:2211501/1‑136 (MQ=255)

                 aCGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATAGCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTGTTTTTTTTTTAATTTATAA‑AAATTTGTAAAATAA‑GGGaa                                                                                                                   >  2:1841881/1‑140 (MQ=255)

                 aCGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTTTTATTTTTTATGTTATTTATTA‑AAATTTTTAAAAAAA‑GGGaa                                                                                                                   >  2:551722/1‑140 (MQ=255)

                 aCGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGTTTTTGTTGGTTTTTTTGTTATTACTAC‑AAATTTGTAAAAttagggga                                                                                                                    >  2:1880645/1‑133 (MQ=255)

                    gCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGTTTTTTTTTTTTTTTTTTTTATTTAGAA‑AAAATTGTAAAATAA‑GGGcaaaa                                                                                                                >  1:2781800/1‑140 (MQ=255)

                     cTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGTTTTTGTTGTTTTTTATTTTATTAAGAA‑AAAATTTAAAAATTC‑GGGTAAAAc                                                                                                               >  2:71061/1‑140 (MQ=255)

                     cTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGTTTTTGTTGTTTTTTATGTAAATAATAC‑AAATTTTTAAAATAA‑GGGAAAAAc                                                                                                               >  2:4151266/1‑140 (MQ=255)

                      ttGCTGACTCGGTGCAGAGCGGTTAAATTTTTAAATTTATACCGATTTAAACAAAGTAAAACAAACACGCCTGGTAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTAC‑AAATTTGAAAAATAC‑GGGAAAAAC‑a                                                                                                             <  2:1504406/140‑1 (MQ=255)

                             aTCGGTGCAGAGCGGGTAAATTTTTAAATTTATACCTATTGAAACAAAGTAACACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTAC‑AAATTTGAAAAATAC‑GGGAAAAAC‑AT‑CCCAtt                                                                                                     <  1:1031920/140‑1 (MQ=255)

                             aTCCGTGCAGAGCGGGGAACTTTTTAAATTTATCCCGATTGAAACAAAGCAAAAAAAACACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTAC‑AAATTTGAAAAATAC‑GGGAAAAAC‑AT‑CCCAtt                                                                                                     <  1:1032024/140‑1 (MQ=255)

                               cGGTGCAGAGCTGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTAC‑AAATTTGAAAAATAC‑GGGAAAAAC‑AT‑CCCATTTa                                                                                                   <  2:801851/140‑1 (MQ=255)

                                 gTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACTCCTGGGAAACAGGGCGTTTTTTTGTTCTTTTTTATGTAAATACCAA‑AAATTTTAAAAATAC‑GGGGAAAAA‑AT‑CCCAATTACt                                                                                                 >  2:1326681/1‑140 (MQ=255)

                                 gTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAACCCGGGCGGGTTGTTTTTCTTTTTTATGTAATTAATAC‑AAATTTTAAAGATAA‑GGGAAAAAA‑AT‑CCCAATTaat                                                                                                 >  2:751947/1‑138 (MQ=255)

                                 gTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGTTGTTGTTCTTTTTTTTGTAATTACTAC‑AAATTTGAAAAATAC‑TGGCAAAAA‑CT‑ACCATTTTCt                                                                                                 >  2:3770641/1‑140 (MQ=255)

                                       agtgtggAAATTTTTAAATTTATACCGATTGAAAAAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTAATAC‑AAATTTGAAAAATAC‑GGGAAAAAC‑AT‑CCCATTTACTTCCaaa                                                                                           <  2:2217711/135‑1 (MQ=255)

                                       agCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGGTTTTGTTGTTTTTTTTTTTATTAGTACGAAATTTTAAAAA‑AA‑GGGAAAAACAAC‑CCCATTTattacaaa                                                                                            >  1:694888/1‑133 (MQ=255)

                                         cGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGTGTTTGTTGTTTGTTTTGTTATTGCTAC‑AAAATTTTAAAATAA‑GGGTACAAA‑CT‑CCCCGTTTCTTCCaaaag                                                                                         >  1:2157079/1‑139 (MQ=255)

                                            ggAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAAGCGGGCGTGTTGTTGTTTTTTTTTTTGTAATTACTAC‑AAATTTTTAAAATAA‑GGGAAAAAC‑AT‑GCCCTTTAATTAAAAAATAtt                                                                                      >  2:775158/1‑140 (MQ=255)

                                            ggAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTGGTTGTTATGTAAATAATAA‑AAATTTTAAAAATAA‑GGGAAAAAA‑AT‑CCCAATTTATTCAAAAATAtt                                                                                      >  2:1429486/1‑140 (MQ=255)

                                            ggAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGTTTTTTTTGTTTTTTATGTTAATAATAC‑AAAATTTAAAAATAA‑CTGAAAAAC‑AT‑ACCCTTTTATTCCAAAATAtt                                                                                      >  1:841291/1‑140 (MQ=255)

                                            ggAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGTTTTTGTTCTTTTTTATTTTATTAATAA‑AAATATTAAAAATAC‑GGGAAAACA‑AT‑CCCATTTACTTCCAACatagt                                                                                      >  2:3070939/1‑138 (MQ=255)

                                                 tttttAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGGTTTGGTTCTTTTTTATGTTATTACTAC‑AAATTTGTAAAATAA‑GGGAAAAAA‑AT‑ACCATTTACTTACAAAATATTTTATg                                                                                 >  2:2255524/1‑140 (MQ=255)

                                                            ataCCTATTCAAAAAAAGAAAAACAAACACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTAC‑AAATTTGAAAAATAC‑GGGAAAAAC‑AT‑CCCATTTACTTCCAAAATATTTGATGGTAATTTTATg                                                                      <  1:1176015/140‑1 (MQ=255)

                                                            ataCCGATTCAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTAC‑AAATTTGAAAAATAC‑GGGAAAAAC‑AT‑CCCATTTACTTCCAAAATATTTGATGGTAATTTTATg                                                                      <  2:1365771/140‑1 (MQ=255)

                                                              aCCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTAC‑AAATTTGAAAAATAC‑GGGAAAAAC‑AT‑CCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTa                                                                    <  2:3045012/140‑1 (MQ=255)

                                                              aCCGATAGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTAC‑AAATTTGAAAAATAC‑GGGAAAAAC‑AT‑CCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTa                                                                    <  2:500390/140‑1 (MQ=255)

                                                                 gATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTAC‑AAATTTGAAAAATAC‑GGGAAAAAC‑AT‑CCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGt                                                                 <  1:2262227/140‑1 (MQ=255)

                                                                 gATTGAAAAAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTAC‑AAATTTGAAAAATAC‑GGGAAAAAC‑AT‑CCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGt                                                                 <  1:620946/140‑1 (MQ=255)

                                                                  aTTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTAC‑AAATTTTAAAAATAC‑GGGAAAAAC‑AT‑ACCATTTAATTCCAAAAAATTTTATGGTAATTTAATGtctatt                                                                >  2:3125454/1‑135 (MQ=255)

                                                                  aTTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTAC‑AAATTTGAAAAATAC‑GGGAAAAAC‑AT‑CCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTa                                                                <  2:3003591/140‑1 (MQ=255)

                                                                  aTTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGTTTTTTTTTTTTTTTTTGTTATTTATAA‑AAAATTTAAAAAAAA‑GGGAAAAAA‑ATAGCCATTAATTACCAAAAAATTTTTTGTTATTTTTTTGtgtgt                                                                 >  1:3459865/1‑136 (MQ=255)

                                                                  aTTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGTTTTTGTTTTTTTTTTTTTTATTACTAA‑AAAATTTTAAAATACTGGGAAAAAA‑GA‑CCCTGTTA‑TTCGCAAATATTTTATGGTTATTTTAttgaggga                                                                >  1:1119287/1‑133 (MQ=255)

                                                                   ttGAAACAAAGTAAAACAAAAACGCCGGGGAAACCGGGCGTTTTTTTGTTATTTGTTATGTAATTACTTA‑AAATATGAAAAATAA‑GGGCACAAA‑AT‑ACCCTTTTATTCCATAATATATGTTGGTAATTTTATggcggtct                                                               >  2:4084094/1‑133 (MQ=255)

                                                                      aaaCAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATCTAATTACTAC‑AAATTTGAAAAATAC‑GGGAAAAAC‑AT‑CCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAAt                                                            <  1:3127500/140‑1 (MQ=255)

                                                                             gaaccacaacaCCGCCTGGGAAACCGGGCCTTTTATGGTTCTATTTTATGTAATTACTAC‑ACAATTGAAAACTAC‑GGGAAAAAC‑AT‑CCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTAtt                                                     <  1:4116135/135‑1 (MQ=255)

                                                                             ccaaaaCAACAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTAC‑ACATTTGAAAAATAC‑GGGAAACAC‑AT‑CCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTAtt                                                     <  1:4244274/138‑1 (MQ=255)

                                                                                acaCAAACACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTAC‑AAATTTGAAAAATAC‑GGGAAAAAC‑AT‑CCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                                                  <  1:260238/138‑1 (MQ=255)

                                                                                aaacaaacACGCCTGTGAAACCTGGCGTTTTTTTGTTCTTTTTTATGTAATTACTAC‑AAATTTGAAAAATAC‑GGGAAAAAC‑AT‑CCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                                                  <  1:1459799/140‑1 (MQ=255)

                                                                                aaacaaacACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTAC‑AAATTTGAAAAATAC‑GGGAAAAAC‑AT‑CCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                                                  <  2:1055001/140‑1 (MQ=255)

                                                                                aaacaaacACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTAC‑AAATTTGAAAAATAC‑GGGAAAAAC‑AT‑CCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                                                  <  2:914078/140‑1 (MQ=255)

                                                                                aaacaaacACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTAC‑AAATTTGAAAAATAC‑GGGAAAAAC‑AT‑CCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                                                  <  1:1817460/140‑1 (MQ=255)

                                                                                aaacaaacACGCATGGGAAACCGGGCGTTTTTTTGTTGTTTTTTATTTAATTACTAC‑AACTTTGAAAAATAC‑GGGAAAAAC‑AT‑CCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTATGTAAAATTTGTATTTgg                                                  <  2:2639403/140‑1 (MQ=255)

                                                                                aaaaaaACACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTAC‑AAATTTGAAAAATAC‑GGGAAAAAC‑AT‑CCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                                                  <  1:3506312/140‑1 (MQ=255)

                                                                                aaaCACAAACGCCGGGGACACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTAC‑AAATGTGAAAAATAC‑GGGAAAAAC‑AT‑CCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                                                  <  1:3366243/140‑1 (MQ=255)

                                                                                aaaCAACCACTCCTGTGACACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTAATAC‑ACATTTGAAAAATAC‑GGGAAAAAC‑AT‑CCCATTTACTTCCAAAATATTTGATGTTAATTTTATGTAGGTAAAATTTGTATTTgg                                                  <  1:3911628/140‑1 (MQ=255)

                                                                                aaaCAACCACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTAC‑AAATTTGAAAAATAC‑GGGAAAAAC‑AT‑CCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                                                  <  2:1987969/140‑1 (MQ=255)

                                                                                aaaCAACAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTAC‑AAATTTGAAAAATAC‑GGGAAAAAC‑AT‑CCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                                                  <  1:91522/140‑1 (MQ=255)

                                                                                aaaCAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTAC‑AAATTTGAAAAATAC‑GGGAAAAAC‑AT‑CCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                                                  <  1:224105/140‑1 (MQ=255)

                                                                                aaaCAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTAC‑AAATTTGAAAAATAC‑GGGAAAAAC‑AT‑CCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                                                  <  1:3959517/140‑1 (MQ=255)

                                                                                aaaCAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTAC‑AAATTTGAAAAATAC‑GGGAAAAAC‑AT‑CCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                                                  <  1:1728455/140‑1 (MQ=255)

                                                                                aaaCAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTAC‑AAATTTGAAAAATAC‑GGGAAAAAC‑AT‑CCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                                                  <  1:1459820/140‑1 (MQ=255)

                                                                                aaaCAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTAC‑AAATTTGAAAAATAC‑GGGAAAAAC‑AT‑CCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                                                  <  2:2773563/140‑1 (MQ=255)

                                                                                aaaCAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTAC‑AAATTTGAAAAATAC‑GGGAAAAAC‑AT‑CCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                                                  <  1:724300/140‑1 (MQ=255)

                                                                                 aaCAAAAACGCCTGGGAAACCGGGCGTGTTTTTTTTGTTTTTTATGTTATTACTAA‑AAATTTTTTAAATAC‑GGGCAAAAA‑AT‑ACCATTTAATTCCAAAAAATTTTATGGTAATTTTGTTTGTGTTAAATTTTTATTTTGc                                                 >  2:1548357/1‑140 (MQ=255)

                                                                                        accccTGGGAAACCGGGCGATTATTTGTTATTTTTTATGTAATTACTAC‑AAATTTGAAAAATAC‑GGGAAAAAC‑AT‑CCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCag                                          <  1:338046/137‑1 (MQ=255)

                                                                                        aCGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTAC‑AAATTTGAAAAATAC‑GGGAAAAAC‑AT‑CCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCag                                          <  1:3584384/140‑1 (MQ=255)

                                                                                        aCGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTAC‑AAATTTGAAAAATAC‑GGGAAAAAC‑AT‑CCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCag                                          <  1:1489699/140‑1 (MQ=255)

                                                                                        aCGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTAC‑AAATTTGAAAAATAC‑GGGAAAAAC‑AT‑CCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCag                                          <  1:1919472/140‑1 (MQ=255)

                                                                                          gCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTAC‑AAATTTGAAAAATAC‑GGGAAAAAC‑AT‑CCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCagag                                        <  2:1127704/140‑1 (MQ=255)

                                                                                          gCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTAC‑AAATTTGAAAAATAC‑GGGAAAAAC‑AT‑CCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCagag                                        <  1:3633913/140‑1 (MQ=255)

                                                                                          gCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTAC‑AAATTTGAAAAATAC‑GGGAAAAAC‑AT‑CCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCagag                                        <  1:1898412/140‑1 (MQ=255)

                                                                                          gCCTGGGAAACCGGGCGTTTTATTGTTCTTTTTTATGTAATTACTAC‑AAATTTGAAAAATAC‑GGGAAAAAC‑AT‑CCCATTTACTTCCAAAATATTGGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCagag                                        <  2:1220458/140‑1 (MQ=255)

                                                                                          gCCTGCGAAACCGGGCGAGATGTTGTTCCTTTTTATGTAATGACGAC‑ACATGGGAAAAATAC‑GGGAAAAAC‑AT‑CCCATTTACTACCAAAATATGTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCagag                                        <  2:2648633/140‑1 (MQ=255)

                                                                                                      gggCGTGTTTTTTTTGTTTTTTTTTTTATTACAAA‑AAATTTGAAAAATAA‑GGGGAAAAA‑AA‑CACAATTACTTACAAAAAAATTTATGGTAATTTTATGTAGGTAAAAATTGTATTTTGACGTTGAGAGGAGGTGaaaaac                            >  2:384194/1‑138 (MQ=255)

                                                                                                         cGTTTTTTTGTTCTTTTTTATGTAATTACTAC‑AAATTTGAAAAATAC‑GGGAAAAAC‑AT‑CCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCggg                         <  1:3616337/140‑1 (MQ=255)

                                                                                                         cGTTTTTTTGTTCTTTTTTATGTAATTACTAC‑AAATTTGAAAAATAC‑GGGAAAAAC‑AT‑CCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCggg                         <  2:638631/140‑1 (MQ=255)

                                                                                                         cGTTTTTTTGTTCTTTTTTATGTAATTACTAC‑AAATTTGAAAAATAC‑GGGAAAAAC‑AT‑CCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCggg                         <  1:2973711/140‑1 (MQ=255)

                                                                                                         cGTTTTTTTGTTATTTTTTATGTAATTACTAC‑AAATTTGAAAAATAC‑GGGAAAAAC‑AT‑CCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCggg                         <  2:1493546/140‑1 (MQ=255)

                                                                                                         cGTTTTTTTGTTATATTTTATGTACTTACTAC‑AAATTTGAAAAATAC‑GGGAAAAAC‑AT‑CCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCggg                         <  1:384426/140‑1 (MQ=255)

                                                                                                                    tCTTTTTTATGTAATTACTAC‑AAATTTGAAAAATAC‑GGGAAAAAC‑AT‑CCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCGGGCAGGCCTGAat              <  2:1914877/140‑1 (MQ=255)

                                                                                                                           tATGTAATTACTAC‑AAATTTGAAAAATAC‑GGGAAAAAC‑AT‑CCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGTTGAAAAGCGGGCAGGCCTGAATATTTTta       >  2:4172910/1‑140 (MQ=255)

                                                                                                                           tATGTAATTACTAC‑AAATTTGAAAAATAC‑GGGAAAAAC‑AT‑CCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCGGGCAGGCCTGAATATTTTta       >  2:34768/1‑140 (MQ=255)

                                                                                                                           tATGTAATTACTAC‑AAATTTGAAAAATAC‑GGGAAAAAC‑AT‑CCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCGGGCAGGCCTGAATATTTTta       >  2:1950750/1‑140 (MQ=255)

                                                                                                                           tATGTAATTACTAC‑AAATTTGAAAAATAC‑GGGAAAAAC‑AT‑CCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCGGGCAGGCCTGAATATTTTta       >  2:1836556/1‑140 (MQ=255)

                                                                                                                           tATGTAATTACTAC‑AAATTTGAAAAATAC‑GGGAAAAAC‑AT‑CCCATTTACTTCCAAAAGATTTGATGGTAATTTTATGTAGTTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCGGGCAGGCCTGAATATTTTta       >  2:1836586/1‑140 (MQ=255)

                                                                                                                            aTGTAATTACTAC‑AAATTTGAAAAATAC‑GGGAAAAAC‑AT‑CCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCGGGCAGGCCTGAATATTTTtat      <  1:2738280/140‑1 (MQ=255)

                                                                                                                            aTGTAATTACTAC‑AAATTTGAAAAATAC‑GGGAAAAAC‑AT‑CCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCGGGCAGGCCTGAATATTTTtat      <  1:6515/140‑1 (MQ=255)

                                                                                                                                aaTTACTAC‑AAATTTGAAAAATAC‑GGGAAAAAC‑AT‑CCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCGGGCAGGCCTGAATATTTTTATAACt  >  2:3929350/1‑140 (MQ=255)

                                                                                                                                aaTTACTAC‑AAATTTGAAAAATAC‑GGGAAAAAC‑AT‑CCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCGGGCAGGCCTGAATATTTTTATAACt  >  1:579427/1‑140 (MQ=255)

TACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACTAC‑AAATTTGAAAAATAC‑GGGAAAAAC‑AT‑CCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCGGGCAGGCCTGAATATTTTTATAACT  >  NZ_CP034943/2991119‑2991386

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`