BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	3,851,230	G→T	7.8%	Q49K (CAG→AAG)	EO946_RS19820 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	3,851,230	0	G	T	7.8%	722.3 / 16.7	279	Q49K (CAG→AAG)	EO946_RS19820	hypothetical protein
Reads supporting (aligned to +/- strand): ref base G (175/71); new base T (2/31); total (177/102)
Fisher's exact test for biased strand distribution p-value = 2.70e-13
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 284 total aligned reads displayed.

AGTCCTGCAATGTCCCAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGC‑AAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATT  >  NZ_CP034943/3851091‑3851369

 gTCCTGCAATGTCCCAATCTGCTCTCTGTACTATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGt                                                                                                                                             >  2:1833095/1‑140 (MQ=255)

 gTCCTGCAATGTCCCAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGt                                                                                                                                             >  2:526702/1‑140 (MQ=255)

 gTCCTGCAATGTCCCAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGt                                                                                                                                             >  2:3584682/1‑140 (MQ=255)

    cTGCAATGTCCCAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCGTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATc                                                                                                                                          >  1:2894024/1‑140 (MQ=255)

          tGTCCCAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGAtt                                                                                                                                    <  1:3421873/140‑1 (MQ=255)

            tCCCAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTg                                                                                                                                              >  2:2803894/1‑128 (MQ=255)

            tCCCAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCg                                                                                                                                  >  1:3718522/1‑140 (MQ=255)

            tCCCAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCg                                                                                                                                  >  1:4163345/1‑140 (MQ=255)

            tCCCAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCg                                                                                                                                  >  2:1893069/1‑140 (MQ=255)

            tCCCAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCg                                                                                                                                  >  1:2526261/1‑140 (MQ=255)

            tCCCAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCg                                                                                                                                  >  2:2675657/1‑140 (MQ=255)

            tCCCAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCg                                                                                                                                  >  1:1203060/1‑140 (MQ=255)

            tCCCAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCg                                                                                                                                  >  2:3140361/1‑140 (MQ=255)

               cAATGTGCTCTCTGTAATATGGCTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATc                                                                                                                               <  2:2299933/140‑1 (MQ=255)

               cAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTTTTACCTGTATCATGATTCGATc                                                                                                                               <  2:1628984/140‑1 (MQ=255)

               cAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATc                                                                                                                               <  1:3584682/140‑1 (MQ=255)

               cAATATGCTATCTGTAATATTGATTTTTAATGGAATTCTCTTTTCTCTAGTTTTATCACCTTGTAAGCATAACCTTCTATGTACTCGGACAGGTCTTCTATTAATGAACCAGGCGTTTTTACCTGTATCATGATTCGATc                                                                                                                               <  2:1805092/140‑1 (MQ=255)

                aaTCTGCTCTCTGTTATAGTGATTGTAAATGGATTTCTCTTTTCGCTAGTTTTATCAACTTGTAAGCATAACCTACAAAGTACTCGGACAGGTCGTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCg                                                                                                                              <  2:3768125/140‑1 (MQ=255)

                aaTCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCg                                                                                                                              <  1:2595936/140‑1 (MQ=255)

                      ctctctGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACATGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATAGTATGC‑a                                                                                                                       >  2:2579630/1‑140 (MQ=255)

                      ctctctGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCg                                                                                                                              <  1:949248/134‑1 (MQ=255)

                      ctctctGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGC‑a                                                                                                                       >  1:187280/1‑140 (MQ=255)

                      ctctctGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGC‑a                                                                                                                       >  1:2256670/1‑140 (MQ=255)

                      ctctctGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGC‑a                                                                                                                       >  2:1062143/1‑140 (MQ=255)

                      ctctctGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTGTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGC‑a                                                                                                                       >  1:1924970/1‑140 (MQ=255)

                          ctGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGC‑AAGcc                                                                                                                   <  2:2948811/140‑1 (MQ=255)

                          ctGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGC‑AAGcc                                                                                                                   <  2:3015697/140‑1 (MQ=255)

                          ctGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGC‑AAGcc                                                                                                                   <  2:2218217/140‑1 (MQ=255)

                          ctGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGC‑AAGcc                                                                                                                   <  2:2759213/140‑1 (MQ=255)

                           tGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGC‑AAGccc                                                                                                                  >  1:849664/1‑140 (MQ=255)

                           tGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTAACTGTATCATGATTCGATCGTATGC‑AATccc                                                                                                                  >  1:1268516/1‑140 (MQ=255)

                              aaTATGGATTGGAAATGGAATTCTCTTGTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGC‑AAGCCCTTc                                                                                                               >  1:313164/1‑140 (MQ=255)

                                tatGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGC‑AAGCCCTTccc                                                                                                             >  1:915190/1‑140 (MQ=255)

                                     aTTGGAAATGGCATTCTCTTTTCGCTAGTTTTATCACCTGGTACGCCTAACCTGCACTGTACTCGGACAGGGCTTCGATGATTGAACCCGTCGTTATTCCGTGGAGCGGGATTCGCTCGTATGC‑AAGCCCTTCCCaaaaa                                                                                                        >  2:3325412/1‑140 (MQ=255)

                                     aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGC‑AAGCCCTTCCCaaaaa                                                                                                        >  2:105365/1‑140 (MQ=255)

                                     aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGC‑AAGCCCTTCCCaaaaa                                                                                                        >  1:997507/1‑140 (MQ=255)

                                     aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGC‑AAGCCCTTCCCaaaaa                                                                                                        >  1:1100581/1‑140 (MQ=255)

                                     aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGC‑AAGCCCTTCCCaaaaa                                                                                                        >  1:1817169/1‑140 (MQ=255)

                                     aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGC‑AAGCCCTTCCCaaaaa                                                                                                        >  1:2550717/1‑140 (MQ=255)

                                     aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGC‑AAGCCCTTCCCaaaaa                                                                                                        >  1:2934414/1‑140 (MQ=255)

                                     aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGC‑AAGCCCTTCCCaaaaa                                                                                                        >  1:1286032/1‑140 (MQ=255)

                                       tGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTCTTACCTGTATCATGATTCGATCGTATGC‑AAGCCCTTCCCAAAAACg                                                                                                      >  2:3251679/1‑140 (MQ=255)

                                       tGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGC‑AAGCCCTTCCCAAAAACg                                                                                                      >  1:141224/1‑140 (MQ=255)

                                       tGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGC‑AAGCCCTTACCaaaaaag                                                                                                      >  1:1430414/1‑138 (MQ=255)

                                       tGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTAGATCGTATGC‑AAGCCCTTCCCAAAAACg                                                                                                      >  2:3399520/1‑140 (MQ=255)

                                       tGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTAACAGTATCATTATTAGATAGTATGC‑AAGCCCGTCCCCAAAACg                                                                                                      >  1:3236646/1‑140 (MQ=255)

                                                   tctcTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAATGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGC‑AAGCCCTTCCCAAACACGATAAAACGActc                                                                                          >  2:666950/1‑140 (MQ=255)

                                                    ctctTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGC‑AAGCCCTTCCCAAAAACGATAAAACGActct                                                                                         >  2:2159298/1‑140 (MQ=255)

                                                      ctTTGCGCTTGTTTTAACACCTTGAAACCCAAACCTTAAAAGTACCCGCACTGGTCTTCAAAAAAGAACCCCGTGTTTTTTACCTGTATCATGATTCGAGCGTAGGC‑AACCCCTTCCCAAAAACCAAACCACGACTCTGc                                                                                       <  2:1379093/140‑1 (MQ=255)

                                                       ttttCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGC‑AAGCCCTTCCCAAAAACGATAAAACGACTCTGct                                                                                      >  1:2119253/1‑140 (MQ=255)

                                                       ttttCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATAATGATTCGATCGTATGC‑AAGCCCATCCCAAAAACCATAAAACGACTCTGct                                                                                      >  1:572306/1‑140 (MQ=255)

                                                                           ttGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTTTATCATGATTCGATAGTATGC‑AAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACAt                                                                  >  1:461618/1‑140 (MQ=255)

                                                                            tttAAGCATAACCTTAAATTTACTCGGAATGTTCTTATATAAATCAACCAGTCTTTTTTACCTTTATCATTTTTCGATCTTCGGA‑ACGCCCTGCCCAAACCCCATAACACGACTCTGCTTCTTCAAGTTCGCAAGACATa                                                                 <  2:1121209/138‑1 (MQ=255)

                                                                            tGTAAGCATAACCTTAAAAGTACGCGTAAAGTTCTTCTATAATTTAACCAGTCGTTTTTACCTGTATCATTATTCGAGCGTATGC‑AAGCCCTTCCAAAAAACGATAACACGACTCTGCTTCTTCAAGTTCGCAAGACATa                                                                 <  2:795912/140‑1 (MQ=255)

                                                                                agaaTAACCTTCAAAGTACTCGCACAGTTCTTCTATAAATCAACCCGTCGTTATTACCTGTAACATTATTCGATCGTTTGC‑AAGCCCTCCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATaacaa                                                             <  2:1284436/137‑1 (MQ=255)

                                                                                    tAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGC‑AAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtg                                                         >  2:2074023/1‑140 (MQ=255)

                                                                                    tAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGC‑AAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtg                                                         >  1:4009961/1‑140 (MQ=255)

                                                                                       cctaaaaaTTACTCCCAAAGGTCTTATATAAAGTACCGATGCGTTTTTACCTGTATCTTTATTCGATCTTATGC‑ACGCCCTTCAAAAACACGAAAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtgatg                                                      <  1:2429998/135‑1 (MQ=255)

                                                                                       ccTTCAATGTACTCGGACTGTTCTTCTATAAATTAACCAGTGTTTTTTCCCTGTATATTTTTTCGATCGTATGC‑AAGCCCTTCCAAAAAACGATAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtgatg                                                      <  2:389431/140‑1 (MQ=255)

                                                                                       ccTTAAAAGTACTCGGACTGTTATTCTATAAATGAACCAGTCTTTATTACCTGTATCATGATTCGTTCGTATGC‑CAGCCCTACCCAAAACCTATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtgatg                                                      <  2:573353/140‑1 (MQ=255)

                                                                                       ccTACAAAGTAGTGCCACAGGTCTTATATAAATGAACCAGTCTTTATTCCCTGTATCATCATGCTATCGTATGC‑AACCCCTTCCCAAAAACGATAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtgatg                                                      <  2:2358304/140‑1 (MQ=255)

                                                                                       catccAATGTACTCGCACTGGTTTTCTATAAATTAACCAGTGTTTTTTACATTTATCATGATTCGATCGTTTGC‑ACGCCCTTCCAAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtgatg                                                      <  2:2517111/136‑1 (MQ=255)

                                                                                          tCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGC‑AAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtgatgatg                                                   >  2:2101533/1‑138 (MQ=255)

                                                                                            aaaGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTCTTACCTGTATCCTGATTCGCGCGTATGC‑CAGCCCGTCCCAAAAACGATAACACGACTCTGCTTGTTCCACTTCGCAAGACAGACACACGTGATGAGGCt                                                 >  1:622845/1‑140 (MQ=255)

                                                                                            aaaGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGC‑AAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACCTAACAACTTGATGAGGCt                                                 >  1:1591163/1‑140 (MQ=255)

                                                                                            aaaGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGC‑AAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCt                                                 >  1:3783697/1‑140 (MQ=255)

                                                                                            aaaGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGC‑AAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCt                                                 >  2:1737300/1‑140 (MQ=255)

                                                                                            aaaGTACTCGGACAGGTCTTCTATAAATGAACAAGTCGTTATTAACTGTATAATGATTCGATAGTATGC‑AAGCCCTTCCCAAAAAAGATAAAACGACTCAGCTTCTTAAACTTAGCAAGACAATACAAACTGATGAGGCt                                                 >  1:3356687/1‑140 (MQ=255)

                                                                                             aatcaCTCGCACAGGTCTTCTAAAAATCACCCCGTCTTTATAACCTGTATAATGATTCTATCGTATGC‑ACGCCCTCCCCAAAACCCATAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCtt                                                <  1:4043614/136‑1 (MQ=255)

                                                                                                  cTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGC‑AAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAagag                                           >  2:198565/1‑140 (MQ=255)

                                                                                                  cTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGC‑AAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAagag                                           >  2:462494/1‑140 (MQ=255)

                                                                                                  cTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATAGTATGC‑AAGCCCTTCCCAAATACGATAAAACGCCTCTGCTTCTTCAATATAGCAAGACCAAACAACTTGATGAGGCTTAacag                                           >  1:136256/1‑140 (MQ=255)

                                                                                                   tCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGC‑AAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGc                                          >  2:4261385/1‑140 (MQ=255)

                                                                                                     ggACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCAAGATTAGATCGTATGA‑AAGCCCGTCCCAAAAACGATAAAACCACACAGATTCTTAAAGTTCTCAAGACATAAAAACTTTTTGATGCTTAAGAGCGa                                        >  1:2321103/1‑140 (MQ=255)

                                                                                                      gaacggTCTAGTATAAATCAACCCGTCTTTTTTACCTGTATCTTTATTCGTTGTGATCA‑AACCCGTGCCAAAAAACTATAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGAt                                       <  1:3202441/136‑1 (MQ=255)

                                                                                                      cactgctcttatATAAATCAACCTGTGTTTATCACTTGTATCATTTTACCACCTTAGGA‑AAGCCCAACCCAAAAACTAAAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGAt                                       <  1:2732987/134‑1 (MQ=255)

                                                                                                      caccggTATTCAATAAATCAACCTGTCTTTATTACCTTTAACTTGTTTCGATCGTCTGC‑ACGCCGTCCCAAAAAACCAAAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGAt                                       <  1:105365/136‑1 (MQ=255)

                                                                                                      caCAGCTCTTCTATAAATCAACCACTCTTTATTACCGTAAAAATGATGCTATC‑TAAGCAAAGCCCTACCAAAAACCCAAAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGAt                                       <  2:2781504/139‑1 (MQ=255)

                                                                                                          ggTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGC‑AAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAAAAAATTGATGAGGCTTAAGAGCGATCATg                                   >  2:4140524/1‑140 (MQ=255)

                                                                                                          ggTCTTCTATAAATGAACCAGTCGTGATTACCGGTATCATGATTAGATCGTATGC‑AATCCCTTCCCAAAAAAGATAAAACGACTCTGCTTCTTCAATTTCGCAAGACATAACAACTTGATGAGGCGTAAGAGctgtctct                                   >  1:1301759/1‑133 (MQ=255)

                                                                                                                ttaaatttGAAACAGTTGTTATTAATTTTATATTGTTTTGAGCGTATGA‑ATGCACTTCCCAAAAACTATAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTaaa                             <  1:731050/133‑1 (MQ=255)

                                                                                                                ataaaaaTCAACCTGTGTTTTTTACTTTTATGATTATTCGATGGTTGGA‑ACGCCCTCCCAAACCCCCATAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTaaa                             <  1:1726110/136‑1 (MQ=255)

                                                                                                                 tataAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATTC‑AAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAAt                            >  1:3027135/1‑140 (MQ=255)

                                                                                                                  ataAATCAACCATTCGTTTTTACTTTTAACATTTTTCGATTGTATGA‑AAGCCCTACCCAAACCCCATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAAtt                           <  2:1299949/140‑1 (MQ=255)

                                                                                                                    aaaTGAACCAGTCTTTATTACCTTTATCTTGATTCGATCGTCTGA‑AAGCCCGTCCCAAAACCGATAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGc                         <  2:495772/140‑1 (MQ=255)

                                                                                                                      aTGAACCAGTCGTTATTACCTGTATCATGATTCGATCGGATGC‑AAGCCCTTGCCAAAAACGATAAAACGAAACTGCTTATTCAAGTTAGCAATTCATTACAAAATGCTGAGGCTTAAGATCGATCATTTATAAAATGACg                       >  1:2697108/1‑140 (MQ=255)

                                                                                                                      aTGAACCAGTCGTTATTACCTGTATCATGATTCGATAGTATGC‑AAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTAAAGTTAGCAAGACATAACAACTTGATGAGGCTTTAGAGCGATAATGTCTAAATTGCCg                       >  1:1689743/1‑140 (MQ=255)

                                                                                                                      aTGAACCAGTCGTTATTAACTGTATAATGATTCGATCGTATGC‑AAGCCCTTACCAAAAACGATAAAAAGACTCTGCTTCTTCAATTTAGCAAGACATAACAACTTGATGAGGGTTAATAGCGATCATGTCTAAATTGCCg                       >  1:3928441/1‑140 (MQ=255)

                                                                                                                        caaCCAGTCTTTATCACCTTTATCATTATGCGATCGTCTGC‑ACGCCCTTCCAAAAAACTATAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGAt                     <  2:1705780/139‑1 (MQ=255)

                                                                                                                         aaCCTGTCTTTATTACCTTTATCATTTTTCGTTGGTATGC‑AAGCCCTACCAAAAAACCATAAAACGACTCTGCTTATTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATa                    <  2:4179023/140‑1 (MQ=255)

                                                                                                                         aaCCCGTCTTTATTACGTGTATCATGATTCTATGTTATGC‑ACGCCCTACCCAAAAACGATAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATa                    <  1:2857758/140‑1 (MQ=255)

                                                                                                                         aaCCAGTCTTTATTACCTGTATAATGATTCTATCGTATGC‑AACCCCTTCCAAAAACCTATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATa                    <  2:915190/140‑1 (MQ=255)

                                                                                                                         aaCCAGTCGTTTTTCCCTGTATCATTTTTCGATCGTATGA‑AAGCCCTACCCAAAAACCATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATa                    <  2:1416527/140‑1 (MQ=255)

                                                                                                                         aaCCAGTCGTTATTACCTGTAGAATGATTCTATCTTATCC‑AACCCCTGCCAAAAAACTAAAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATa                    <  2:1198520/140‑1 (MQ=255)

                                                                                                                          aCCAGTCGTTATTACCTGTATCATGAGTCGATCGTATGC‑AAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCCAGTTCGCAACACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCCATAc                   >  1:2909232/1‑140 (MQ=255)

                                                                                                                           ccAGTCTTTTTTACCTTTATATTTATTCTATCGTATGC‑ACGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACt                  <  2:866982/140‑1 (MQ=255)

                                                                                                                              gTCGTTATTACCTGTATCATGATTCGATCGTATGC‑AAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTTATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCt               >  2:3491587/1‑140 (MQ=255)

                                                                                                                               tCGTTATTACCTGTATCATGATTCGATCGTATGC‑AAGCCCTTCCCAAAAACGATAAAACGACGCTGCTTCTTCAAGTTCGCAAGACATAAAAACTTGATGAGGCTTAAGAGCGATCCTGTCTAAATTGCCGATAATGCtt              >  1:3170036/1‑140 (MQ=255)

                                                                                                                                cGTTATAACCTTTATAATGATTCGATCGTATGA‑AAGCCCTTCCAAAAACCGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTta             <  2:4242366/140‑1 (MQ=255)

                                                                                                                                           gTATCATGATTCGATCGTATGC‑AAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTAtt  >  2:3996302/1‑140 (MQ=255)

AGTCCTGCAATGTCCCAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGC‑AAGCCCTTCCCAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATT  >  NZ_CP034943/3851091‑3851369

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`