BRESEQ :: Evidence

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	2,991,245	0	G	T	26.1%	190.4 / 27.8	119	intergenic (+46/‑161)	gbsR/bslA	transcriptional regulator GbsR/biofilm‑surface layer protein BslA
Reads supporting (aligned to +/- strand): ref base G (35/53); new base T (30/1); total (65/54)
Fisher's exact test for biased strand distribution p-value = 6.88e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 120 total aligned reads displayed.

AGCTCTAAAGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAA‑ACGCCTGGGAAACCGGGCGT‑TTTTTTGTTCTTTTTTATGTAATTACTACAAATTTG‑AAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCGGGCAGGCCTGAATATTTTTATAA  >  NZ_CP034943/2991107‑2991384

aGCTCTAAAGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAA‑ACGCCTGGGAAACCGGGCGT‑TTTTTTTTTATTTTTTATGt                                                                                                                                             >  2:577956/1‑140 (MQ=255)

aGCTCTAAAGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAA‑ACGCCTGGGAAACCGGGCGT‑TTTTTTGTTGTTTTTTATGt                                                                                                                                             >  2:275906/1‑140 (MQ=255)

  ctctCAAGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAA‑ACGCCTGGGAAACCGGGCGT‑TGTTTTGTTGTTTTTTATGTaa                                                                                                                                           >  2:1613705/1‑140 (MQ=255)

  ctctAAAGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAA‑ACGCCTGGGAAACCGGGCGT‑TTTTTTGTTCTTTTTTATTTaa                                                                                                                                           >  2:1890590/1‑140 (MQ=255)

  ctctAAAGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAA‑ACGCCTGGGAAACCGGGCGT‑GTTTTTTTTGTTTTTTTTGTaa                                                                                                                                           >  2:1324972/1‑140 (MQ=255)

  ctctAAAGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAA‑ACGCCTGGGAAACCGGGCGT‑GTTTTTGTTGTTTTTTTTTTaa                                                                                                                                           >  1:1493250/1‑140 (MQ=255)

    ctAAAGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAA‑ACGCCTGGGAAACCGGGCGT‑GTTTTTGTTGTTTTTTTTTTAAtt                                                                                                                                         >  1:158329/1‑140 (MQ=255)

     tAAAGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAA‑ACGCCTGGGAAACCGGGCGT‑GTTTTTTTTGTTTTTTATTttatta                                                                                                                                        >  1:2284723/1‑140 (MQ=255)

     tAAAGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAAAAAAA‑ACGCCTGGGAAAACGGGCGT‑TTTTTTGTTTTTTTTTATTttatta                                                                                                                                        >  2:1488302/1‑140 (MQ=255)

            tACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAA‑ACGCCTGGGAAACCGGGCGT‑TTTTTTTTTGTTTTTTTTTTAATTAATAaaaa                                                                                                                                 >  2:1850530/1‑140 (MQ=255)

            tACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAA‑ACGCCTGGGAAACCGGGCGT‑TTTTTTTTTGTTTTTTATTTAATTACTACaaa                                                                                                                                 >  2:1170309/1‑140 (MQ=255)

            tACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAA‑ACGCCTGGGAAACCGGGCGT‑TTTTTTTTTATTTTTTATGTAATTAATAaaaa                                                                                                                                 >  2:1832056/1‑140 (MQ=255)

            tACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAA‑ACGCCTGGGAAACCGGGCGT‑TTTTTTTTTATTTTTTATGTAATTAATACaaa                                                                                                                                 >  2:1850234/1‑140 (MQ=255)

            tACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAA‑ACGCCTGGGAAACCGGGCGT‑TTTTTTGTTGTTTTTTTTTTTATTAATAaaaa                                                                                                                                 >  1:2090291/1‑140 (MQ=255)

            tACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAA‑ACGCCTGGGAAACCGGGCGT‑GTTTTTTTTTTTTTTTTTGTTAATAATAaaaa                                                                                                                                 >  1:14805/1‑140 (MQ=255)

            tACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAA‑ACGCCTGGGAAACCGGGCGT‑GTTTTTTTTGTTTTTTATTTAATAAATAaaaa                                                                                                                                 >  1:19144/1‑140 (MQ=255)

            tACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAA‑ACGCCTGGGAAACCGGGCGT‑GTTTTTGTTGTTTTTTATGTAAATACTACaaa                                                                                                                                 >  2:2369181/1‑140 (MQ=255)

            tACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAA‑ACGCCTGGGAAACCGGGCGT‑GTTTTTGGTGTTTTTTTTGTAATTAATACaaa                                                                                                                                 >  1:1205990/1‑140 (MQ=255)

            tACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAA‑ACGCCTGGGAAAACGGGCGT‑TTTTTTGTTGTTTTTTATTTAAATAATAaaaa                                                                                                                                 >  2:357947/1‑140 (MQ=255)

             aCTCTGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAA‑ACGCCTGGGAAACCGGGCGT‑GTGTTTTTTTTTTTTTTTTTTATTAATAaaaaa                                                                                                                                >  1:783800/1‑139 (MQ=255)

             aCTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAA‑ACGCCTGGGAAACCGGGCGT‑TTTTTTGTTGTTTTTTATGTAATTACTACAAAt                                                                                                                                >  2:2105659/1‑140 (MQ=255)

             aCTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAA‑ACGCCTGGGAAACCGGGCGT‑GTTTTTTTTGTTTTTTATTTAAATAATAaaaaa                                                                                                                                >  1:681692/1‑139 (MQ=255)

             aCTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAA‑ACGCCTGGGAAACCGGGCGT‑GTTTTTGTTTTTTTTTTTTTTATTACTACAAAt                                                                                                                                >  1:1531768/1‑140 (MQ=255)

             aCTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAA‑ACGCCTGGGAAACCGGGCGT‑GTTTTTGTTGTTTTTTCTGTAATTACTACAAAt                                                                                                                                >  2:2108361/1‑140 (MQ=255)

             aCTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAA‑ACGCCTGGGAAACCGGGCGT‑GTTTTTGTTGTTTTTTATGTAATTAATACaaaa                                                                                                                                >  2:2035698/1‑139 (MQ=255)

               tATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAA‑ACGCCTGGGAAACCGGGCGT‑TTTTTTTTTCTTTTTTATTTAATTACTAAAAAttt                                                                                                                              >  2:1065755/1‑140 (MQ=255)

               tATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAA‑ACGCCTGGGAAACCGGGCGT‑TTTTTTGTTATTTTTTATTTAATTAAAAAAAAttt                                                                                                                              >  2:828133/1‑140 (MQ=255)

               tATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAA‑ACGCCTGGGAAACCGGGCGT‑GTTTTTTTTGTTTTTTTTTTAATTAGTACAAAatt                                                                                                                              >  1:142476/1‑140 (MQ=255)

                  gATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAA‑ACGCCTGGGAAACCGGGCGT‑TTTTTTGTTCTTTTTTATGTAATTACTACAAATTTG‑aa                                                                                                                          <  2:915300/140‑1 (MQ=255)

                  gATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAA‑ACGCCTGGGAAACCGGGCGT‑TTTTTTGTTCTTTTTTATGTAATTACTACAAATTTG‑aa                                                                                                                          <  2:915166/140‑1 (MQ=255)

                   aTTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAA‑ACGCCTGGGAAACCGGGCGT‑TTTTTTTTTGTTTTTTATTTAATTACTACAAAATTT‑aaa                                                                                                                         >  1:2281400/1‑140 (MQ=255)

                    ttGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAA‑ACGCCTGGGAAACCGGGCGT‑GTTTTTTTTGTTTTTTTTTTAATTACTAAAAATTTT‑aaaa                                                                                                                        >  1:128495/1‑140 (MQ=255)

                       gCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAA‑ACGCCTGGGAAACCGGGCGT‑TTTTTTGTTTTTTTTTATGTAATTAATACAAATTTT‑AAAAATa                                                                                                                     >  2:503598/1‑140 (MQ=255)

                       gCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAA‑ACGCCTGGGAAACCGGGCGT‑GTTTTTTTTGTTTTTTTTTTAATTAATACAAATTTT‑AAAAATa                                                                                                                     >  2:327750/1‑140 (MQ=255)

                       gCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAA‑ACGCCTGGGAAACCGGGCGT‑GTTTTTGTTGTTTTTTTTTTAAAAAAAACAAATTTA‑AAAAATa                                                                                                                     >  2:1496381/1‑140 (MQ=255)

                         tGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATGTATACCGATTGAAACAAAGTAAAACAAAA‑ACGCCTGGGAAACCGGGCGT‑GTTTGTGTTCTTTTTTATGTAATTACTACAAATTTG‑AAAAATaag                                                                                                                   >  2:1378282/1‑138 (MQ=255)

                           aaaCGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAA‑ACGCCTGGGAAACCGGGCGT‑GTTTTTTTTGTTTTTTATGTAATTACTAAAAATTTG‑TAAAATACggg                                                                                                                 >  2:2093592/1‑140 (MQ=255)

                               ggCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCTATTGAACCAAAGTAAAACAAAA‑ACCCCTGGGAAACCGGGCGT‑TTTTTTGTTCTTTTTTATGTAAGTACTACAAATTTG‑AAAAATACGGGaaaa                                                                                                             <  2:720525/140‑1 (MQ=255)

                               ggCTTGCTGAATCGGTGCAGAGCCGGGAAATTTTTAAATTTATACCGATTTAAAAAAAGTAAAACAAAA‑ACGCCTGGGAAACCGGGCGA‑TTTTTTGTTCTTTTTTATGTAAGTACTACAAATTTG‑AAAAATACGGGaaaa                                                                                                             <  2:721003/140‑1 (MQ=255)

                                 cTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAA‑ACGCCTGGGAAACCGGGCGT‑TTTTTTGTTCTTTTTTATGTAATTACTACAAATTTG‑AAAAATACGGGAAAAAc                                                                                                           <  1:605243/140‑1 (MQ=255)

                                 cTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAA‑ACGCCTGGGAAACCGGGCGT‑GTTTTTGTTGTTTTTTTTGTAATTACTACAAAATTT‑TAAAATAACGGACAAAc                                                                                                           >  1:2022610/1‑140 (MQ=255)

                                 cTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAA‑ACGCCTGGGAAACCGGGCGT‑GTTTTTGGTGTTTTTTTTGTAAATAGAAAAAATTTGTAAAAAAACGGCaaaaa                                                                                                            >  1:445843/1‑140 (MQ=255)

                                  ttGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAA‑ACGCCTGGGAAACCGGGCGT‑GTTTTTGTTGTTTTTTTTGTAATTAAAACAAAATTG‑AAAAAAAAGGGgaaaaaa                                                                                                          >  1:2793072/1‑138 (MQ=255)

                                     cTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAA‑ACGCCTGGGAAACCGGGCGT‑GTTTTTTTTGTTTTTTTTTTTATTAATAAAAAAAAT‑AAAAATACTGGaaaaaaaaca                                                                                                       >  1:1691981/1‑135 (MQ=255)

                                              tGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAA‑ACGCCTGGGAAACCGGGCGT‑GTTTTTTTTGTTTTTTTTGTAATTAATAAAAATTTT‑AAAAATACTGGAAAAAAATCACAGTTACtt                                                                                              >  2:1126555/1‑140 (MQ=255)

                                                 cgcggggggAAATTTTTAAATTTATACCGATTGAAACAACGTAAACAAAAA‑ACGCCTGGGAAACCGGGCGT‑TTTTTTGTTCTTTTTTATGTAATTACTACAAATTTG‑AAAAATACGGGAAAACCATCCCATTTACTTCCa                                                                                           <  1:344770/135‑1 (MQ=255)

                                                 agcgCGGGCAAATTTCAAAATTTAAACCGATTCAAACAAACTAACACAAAA‑ACGCCGGGGAAACCGGGCGT‑TTTTTTGTTCTTTTTTATGTAATTACTACAAATTTG‑AAAAATACGGGAAAAACATCCCATTTACTTCCa                                                                                           <  1:2438416/137‑1 (MQ=255)

                                                 agagCGGGGAAATTTTTAAATTTATACCGATTGAAAAAAAGTAAAACAAAA‑ACGCCTGGGAAACCGGGCGT‑TTTTTTGTTCTTTTTTATGTAATTACTACAAATTTG‑AAAAATACGGGAAAAACATCCCATTTACTTCCa                                                                                           <  1:2035698/140‑1 (MQ=255)

                                                        gtaaaTTTTTAAATTTATACCGATTGAAAAAAAGTAAAAAAAAA‑ACGCCTGGGAAACCGGGCGT‑CTTTTTGTTCTTTTTTATGTAATTACTACAAATTTG‑AAAAATACGGGAAAAACATCCCATTTACTTCCAAAATAtt                                                                                    <  1:1034386/138‑1 (MQ=255)

                                                        ggAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAA‑ACGCCTGGGAAACCGGGCGT‑TTTTTTTTTGTTTTTTTTGTAAATACTACAAATTTT‑TAAAATAAGGGGAAAAAATCCCATTTACTTGCAAAATAtt                                                                                    >  2:384362/1‑140 (MQ=255)

                                                        ggAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAA‑ACGCCTGGGAAACCGGGCGT‑TTTTTTGTTGTTTTTTTTTTAATTACTACCAATTTG‑TAAAATAAGGGAAAAACATCCCATTTAATTCCAAAATAtt                                                                                    >  2:2797176/1‑140 (MQ=255)

                                                        ggAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAA‑ACGCCTGGGAAACCGGGCGT‑GTTTTTTTTCTTTTTTATGTAATTACTAAAAATTTG‑TAAAATACGGGAAAAACATCCCATTTAGTTCCAAAAtttt                                                                                    >  2:2026476/1‑140 (MQ=255)

                                                        ggAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAA‑ACGCCTGGGAAACCGGGCGT‑GTTTTTGTTGTTTTTTATGTAATTAATACAAAATTG‑AAAAAAACGGGAAAAACATCCCATTTAATTCAAAAAAAtt                                                                                    >  2:1687221/1‑140 (MQ=255)

                                                        ggAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAA‑ACGCCTGGGAAACCGGGCGT‑GTTTTAGTTCTTTTTTATGTGATTACTACAAATTTG‑AAAAATAAGTGACAAACATACCAATTCAGTACAAAatata                                                                                    >  2:2639383/1‑139 (MQ=255)

                                                             tttttAAATTTATACCTATTTAAAAAACTTAAAACAAAA‑ACGCCTGGGAAACCGGGATT‑TTTTTTGTTCTTTTTTATGTAATTACTACATATTTG‑AAAAATTGGGGATTAAGATCACATTTAATTCCTAAATAttgtatg                                                                               <  2:1363073/140‑6 (MQ=255)

                                                              ttttAAATTAATACCGATTGAAACAAAGTAAAACAAAA‑ACGCCTGGGAAACCGGGCGT‑TTTTTTGTTCTTTTTTATGTAATTACTACAAATTTG‑AAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATgg                                                                              <  1:266976/140‑1 (MQ=255)

                                                                        cacctcaTTTAAAAAAAGTAAAACAAAA‑ACGCCTGGGAAACCGGGCGT‑TTTTTTGTTCTTTTTTATGTAATTACTACAAATTTG‑AAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATg                                                                    <  1:495376/134‑1 (MQ=255)

                                                                        ataCCGATTTAAACAACGAAAAACAAAA‑ACGCCTGGGAAACCGGGCGT‑TTTTTTGTTCTTTTTTATGTAATTACTCCAAATTTG‑AAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATg                                                                    <  2:486253/140‑1 (MQ=255)

                                                                        ataCCGATTGAAAAAAAGAAAAACAAAA‑ACGCCTGGGAAACCGGGCGT‑TTTTTTGTTCTTTTTTATGTAATTACTACAAATTTG‑AAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATg                                                                    <  2:1918191/140‑1 (MQ=255)

                                                                             gATTCAAACAAAGTAAACCAAAC‑ACGCCTGGGAAACCGGGCGT‑TTTTTTGTTCTTTTTTATGTAATTACTACAAATTTG‑AAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGt                                                               <  1:2369181/140‑1 (MQ=255)

                                                                               ttGAAACAAAGTAAAACAAAA‑ACGCCTGGGAAACCGGGCGT‑TGTTTTGTTCTGTTTTTTGTAATTACTACAAAATTG‑AAAAATACGGGGAAAAAATGCCAGTTTCTTCCCAAAGATTTGATTGTAATTTTTTGTAGGtga                                                             >  2:885271/1‑138 (MQ=255)

                                                                                  aaacaaacAACAACACAA‑ACCCCTGGGAAACCGGGCGT‑TTTTTTGTTCTTTTTTATGTAATTACTACAAATTTG‑AAAAATACGGGAAAAACATACCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAAt                                                          <  1:1832056/140‑1 (MQ=255)

                                                                                    aCAAATTAAAACAAAA‑ACGCCTGGGAAACCGGGCGT‑TTTTTTGTTCTTTTTTATGTAATTACTACAAATTTG‑AAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAAttt                                                        <  2:1146771/140‑1 (MQ=255)

                                                                                        aGTAAAACAAAA‑ACGCCTGGGAAACCGGGCGTGGTTTTTTTTTTTTTTTTTTTATTTATAAAAAATTTA‑AAAAAAAAGGGAAAAAAAACCAGTTTA‑TTACAAAAAATTTTTTTGTAATTTTTTTTTGGTGAAAttttttt                                                    >  1:652160/1‑136 (MQ=255)

                                                                                        aGTAAAACAAAA‑ACGCCTGGGAAACCGGGCGT‑TTTTTTGTTGTTTTTTATTTAATTACTAAAAAATTT‑AAAAATAAGGGTAAAAAAAACCATTTAGTTACAAAATATTTGTTGGTAATTTTATGTTTGTAAAATTTTTAt                                                    >  2:1410815/1‑140 (MQ=255)

                                                                                        aGTAAAACAAAA‑ACGCCTGGGAAACCGGGCGT‑TTTTTTGTTGTTTTTTATGTAATTACTACAAATTTT‑AAAAATAAGGGAAAAACATCCCATTTACTTGCAAAATATTTTATGGTAATTTTATGTAGGTAAAATTTGTAt                                                    >  2:1983105/1‑140 (MQ=255)

                                                                                        aGTAAAACAAAA‑ACGCCTGGGAAACCGGGCGT‑TTTTGTTTTATTTTTTATGTAATTATGAAAAAATTT‑AAAAATAAGGGAAAAACATACCCTTTACTTCCAAAATATTTTATGTTAATTTTATTTAGTTAAAAttttttt                                                    >  2:2464142/1‑136 (MQ=255)

                                                                                        aGTAAAACAAAA‑ACGCCTGGGAAACCGGGCGT‑TGTTTTTTTGTTTTTTATGTAATTACTAAAAATTTG‑TAAAATAAGGGAAAAACATCCCCTTTTATTCCCAAATATTTGTTTGTAATTTTATGTATGTGAAAttgtgtg                                                    >  2:1579105/1‑135 (MQ=255)

                                                                                        aGTAAAACAAAA‑ACGCCTGGGAAACCGGGCGT‑GTTTTTGTTTGTTTTTTTGTTATTACTAAAAAATTT‑AAAAATAAGGGAAAAACATAACAGTTAATTGAAAAAAATTTTTTTTTTATTTTATTTAGTTTATAtttttgt                                                    >  1:1981489/1‑136 (MQ=255)

                                                                                        aGTAAAACAAAA‑ACGCCTGGGAAACCGGGCGT‑GTTTTTGTTGTTTTTTATTTAATTAATACAAAATTT‑TAAAATAAGGGAAAAAAAACCCAATTTCTTCCAAAATATTTTATGGTAATTTTATTTAGGTAAAATTTGttt                                                    >  2:1455866/1‑138 (MQ=255)

                                                                                        aGTAAAACAAAA‑ACGCCTGGGAAACCGGGCGT‑GTTTTTGTTGTTTTTTATTTAAATAATAAAAAATTT‑AAAAATAAGGGAAAAAAATACCATTTAATTACAAAAAATTTTATGGTAATTTTATTTAGGTAAAATTTTTAt                                                    >  2:1481878/1‑140 (MQ=255)

                                                                                         caaacaCAAAA‑ACGCATGGGAAACCGGTCGT‑TTTTTTGTTCTTTTTTATGTAATTACTACAAATTTG‑AAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTAtt                                                   <  1:2673038/135‑1 (MQ=255)

                                                                                            acacaaca‑ACGCCTGGGAAACCGGGCGT‑TTTTTTGTTCTTTTTTATGTAATTACTACAAATTTG‑AAAAATACGGGAAAAACATCCCATGTACTTCCAAAATATTGGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                                                <  2:7907/138‑1 (MQ=255)

                                                                                            aaacaaac‑ACGCCTGGGAAACCGGGCGT‑TTTTTTGTTCTTTTTTATGTAATTACTACAAATTTG‑AAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                                                <  1:2797176/140‑1 (MQ=255)

                                                                                            aaaaaaaa‑ACGCCTGGGAAACCGGGCGT‑TTTTTTGTTCTTTTTTATGTAATTACTACAAATTTG‑AAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                                                <  2:1078721/140‑1 (MQ=255)

                                                                                            aaaaaaaa‑ACGCCTGGGAAACCGGGCGT‑TTTTTTGTTCTTTTTTATGTAATTACTACAAATTTG‑AAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                                                <  2:14805/140‑1 (MQ=255)

                                                                                            aaaaaaaa‑ACGCCTGGGAAACCGGGCGT‑TTTTTTGTTCTTTTTTATGTAATTACTACAAATTTG‑AAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                                                <  2:1558349/140‑1 (MQ=255)

                                                                                            aaaaaaaa‑ACGCCTGGGAAACCGGGCGT‑TTTTTTGTTCTTTTTTATGTAATTACTACAAATTTG‑AAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                                                <  2:290967/140‑1 (MQ=255)

                                                                                            aaaCAAAA‑ACGCCTGGGAAACCGGGCGT‑TTTTTTGTTCTTTTTTATGTAATTACTACAAATTTG‑AAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                                                <  1:1324972/140‑1 (MQ=255)

                                                                                            aaaCAAAA‑ACGCCTGGGAAACCGGGCGT‑TTTTTTGTTCTTTTTTATGTAATTACTACAAATTTG‑AAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                                                <  2:158329/140‑1 (MQ=255)

                                                                                            aaaCAAAA‑ACGCCTGGGAAACCGGGCGT‑TTTTTTGTTCTTTTTTATGTAATTACTACAAATTTG‑AAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                                                <  1:1850530/140‑1 (MQ=255)

                                                                                             aaCAAAACACGCCTGGGAAACCGGGCGT‑TTTTTTGTTCTTTTTTATGTAATTACTACAAATTTG‑AAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                                                <  1:1850234/140‑1 (MQ=255)

                                                                                                       gCCTGGGAAACCGGGCGT‑TTTTTTGTTCTTTTTTATGTAATTACTACAAATTTG‑AAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCagag                                      <  1:2114675/140‑1 (MQ=255)

                                                                                                       gCCTGGGAAACCGGGCGT‑TTTTTTGTTCTTTTTTATGTAATTACTACAAATTTG‑AAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCagag                                      <  1:2818005/140‑1 (MQ=255)

                                                                                                       gCCTGGGAAACCGGGCGT‑TTTTTTGTTCTTTTTTATGTAATTACTACAAATTTG‑AAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCagag                                      <  1:1481878/140‑1 (MQ=255)

                                                                                                       gCCTGGGAAACCGGGCGT‑TTTTTTGTTCTTTTTTATGTAATTACTACAAATTTG‑AAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCagag                                      <  1:384362/140‑1 (MQ=255)

                                                                                                       gCCTGGGAAACCGGGCGT‑TTTTTTGTTCTTTTTTATGTAATTACTACAAATTTG‑AAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCagag                                      <  1:2093592/140‑1 (MQ=255)

                                                                                                       gCCTGGGAAACCGGGCGT‑TTTTTTGTTCTTTTTTATGTAATTACTACAAATTTG‑AAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCagag                                      <  2:19144/140‑1 (MQ=255)

                                                                                                       cccTGGGAAACCGGGCGT‑TTTTTTGTTCTTTTTTATGTAATTACTACAAATTTG‑AAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCagag                                      <  2:188532/139‑1 (MQ=255)

                                                                                                            tgAAAGCGTGCGT‑TTTTTTTTTCTTTTTTATTTAATTAATACAAATTTG‑AAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGt                                 <  1:1687221/139‑1 (MQ=255)

                                                                                                            gcaaaCCGGGCGT‑TTTTTTGTTCTTTTTTATGTAATTACTACAAATTTG‑AAAAATACGGGAAAAACATCCCATTTACGTCCAAACTATTTGAGGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGt                                 <  1:2279903/138‑1 (MQ=255)

                                                                                                                aCCGGGCGT‑TTTTTTGTTCTTTTTTATGTAATTACTACAAATTTG‑AAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGaaa                             <  1:327750/140‑1 (MQ=255)

                                                                                                                 ccGGGCGT‑TTTTTTGTTCTTTTTTATGTAATTACTACAAATTTG‑AAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGaaaa                            <  1:1613705/140‑1 (MQ=255)

                                                                                                                 ccGGGCGT‑TTTTTTGTTCTTTTTTATGTAATTACTACAAATTTG‑AAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGaaaa                            <  1:1065755/140‑1 (MQ=255)

                                                                                                                      cGT‑TTTTTTGTTCTTTTTTATGTAATTACTACATATTTG‑AAAAATACGGGAAACACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCggg                       <  2:2753451/140‑1 (MQ=255)

                                                                                                                      cGT‑TTTTTTGTTCTTTTTTATGTAATTACTACAAATTTG‑AACAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCggg                       <  1:1496381/140‑1 (MQ=255)

                                                                                                                      cGT‑TTTTTTGTTCTTTTTTATGTAATTACTACAAATTTG‑AAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCggg                       <  2:681692/140‑1 (MQ=255)

                                                                                                                                         tATGTAATTACTACAAATTTG‑AAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCGGGCAGGCCTGAATATTTTta     <  1:577956/140‑1 (MQ=255)

                                                                                                                                           tGTAATTACTACAAATTTG‑AAAAATACGGGAAAAACATCCCATTTACTTCCAAAATAGTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCGGGCAGGCCTGAATATTTTtata   >  2:2711889/1‑140 (MQ=255)

                                                                                                                                            gTAATTACTACAAATTTG‑CAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCGGGCAGGCCTGAATATTTTTATaa  <  2:1423259/140‑1 (MQ=255)

AGCTCTAAAGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAA‑ACGCCTGGGAAACCGGGCGT‑TTTTTTGTTCTTTTTTATGTAATTACTACAAATTTG‑AAAAATACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCGGGCAGGCCTGAATATTTTTATAA  >  NZ_CP034943/2991107‑2991384

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`