BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	3,851,220	G→T	7.3%	T52K (ACG→AAG)	EO946_RS19820 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	3,851,220	0	G	T	7.3%	1605.0 / 36.8	574	T52K (ACG→AAG)	EO946_RS19820	hypothetical protein
Reads supporting (aligned to +/- strand): ref base G (381/128); new base T (2/62); total (383/191)
Fisher's exact test for biased strand distribution p-value = 8.71e-31
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 575 total aligned reads displayed.

GAAAGAGAAGTCCTGCAATGTCCCAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAAC‑GATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCT  >  NZ_CP034943/3851083‑3851356

gAAAGAGAAGTCCTGCAATGTCCCAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGtt                                                                                                                                         >  2:825356/1‑140 (MQ=255)

  aaGAGAAGTCCTGCAATGTCCCAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGttat                                                                                                                                       <  1:4803536/140‑1 (MQ=255)

  aaGAGAAGTCCTGCAATGTCCCAAACTGCTCTCTGTAATCTGGCTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATCACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGttat                                                                                                                                       <  2:5773874/140‑1 (MQ=255)

   agagAAGTCCTGCAATGTCCCAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGttatt                                                                                                                                      >  2:3658228/1‑140 (MQ=255)

   agagAAGTCCTGCAATGTCCCAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGttatt                                                                                                                                      >  1:253600/1‑140 (MQ=255)

     agaAGTCCTGCAATGTCCCAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTAc                                                                                                                                    <  2:1838075/140‑1 (MQ=255)

         gTCCTGCAATGTCCCAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGt                                                                                                                                >  1:1274001/1‑140 (MQ=255)

             tGCAATGTCCCAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCa                                                                                                                            <  2:4151040/140‑1 (MQ=255)

             tGCAATGTCCCAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCa                                                                                                                            <  1:6265161/140‑1 (MQ=255)

             tGCAATGTCCCAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCa                                                                                                                            <  2:534542/140‑1 (MQ=255)

             tGCAATGTCCCAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCa                                                                                                                            <  1:5128596/140‑1 (MQ=255)

                  tGTCCCAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGAtt                                                                                                                       <  1:3068896/140‑1 (MQ=255)

                    tCCCAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGAGAGGTCTTCTAGAAATGAACCAGTCGTTATTACCTGTATCTTGATTCg                                                                                                                     >  1:6765221/1‑140 (MQ=255)

                    tCCCAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTct                                                                                                                     >  2:2777871/1‑139 (MQ=255)

                     cccAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGa                                                                                                                    <  1:4685989/140‑1 (MQ=255)

                       cAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATc                                                                                                                  <  2:3260219/140‑1 (MQ=255)

                       cAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATc                                                                                                                  <  2:1981315/140‑1 (MQ=255)

                       cAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGGATCATGATTCGATc                                                                                                                  <  2:5278740/140‑1 (MQ=255)

                        aaTCTGCTCTCTGTAATATGGATTTGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCg                                                                                                                 <  2:619881/140‑1 (MQ=255)

                        aaTCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCg                                                                                                                 <  1:551605/140‑1 (MQ=255)

                        aaTCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCg                                                                                                                 <  1:1109063/140‑1 (MQ=255)

                        aaTCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCg                                                                                                                 <  2:4718698/140‑1 (MQ=255)

                              ctctctGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCt                                                                                                                                  <  2:1207586/117‑1 (MQ=255)

                              ctctctGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATTCa                                                                                                           >  1:2784698/1‑140 (MQ=255)

                              ctctctGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCa                                                                                                           >  1:1767791/1‑140 (MQ=255)

                              ctctctGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCa                                                                                                           >  1:6105217/1‑140 (MQ=255)

                              ctctctGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCa                                                                                                           >  1:2105539/1‑140 (MQ=255)

                              ctctctGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCa                                                                                                           >  1:2711609/1‑140 (MQ=255)

                              ctctctGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCa                                                                                                           >  1:531327/1‑140 (MQ=255)

                              ctctctGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTGATTACCTGTATCATGATTCGATCGTATGCa                                                                                                           >  1:7523409/1‑140 (MQ=255)

                              ctctctGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTAATCGGACAGGTGTTCTATAAATGAACCAGTCTGTATTACCTGTATCATTGTTCGATCGTATGCa                                                                                                           >  2:4259465/1‑140 (MQ=255)

                                 tctGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAACCATAACCTTCAAAGTACTCGGACATGTCTTCTATAAATGAACCAGTCGTTAATACCTGTATCATGATTAGATCGTaagcaagc                                                                                                        >  2:381885/1‑140 (MQ=255)

                                  ctGTAATATGGATTGGAAATGGAATTCTCTTTTCGCGAGTTTTATCACCTTGTAAGCATAACCTTCAAACTACTCGGACATGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGcc                                                                                                       <  1:1565779/140‑1 (MQ=255)

                                   tGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGccc                                                                                                      >  2:7672591/1‑140 (MQ=255)

                                   tGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGccc                                                                                                      >  2:4914148/1‑140 (MQ=255)

                                    gTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCt                                                                                                     >  1:5409218/1‑140 (MQ=255)

                                        tatGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAGTGAACCAGTCGTTAGTACCTGTATCATGAGTAGGTAGTATGCAAGGCCTTccc                                                                                                 >  2:5830415/1‑140 (MQ=255)

                                        tatGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATAATGATTCGATCGTATGCAAGCCATTccc                                                                                                 >  2:6286599/1‑140 (MQ=255)

                                           ggATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCaaa                                                                                              <  1:3671431/140‑1 (MQ=255)

                                           ggATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTAACTGTATCATGATTCGATCGTATGCAAGCCCTTCCCaaa                                                                                              >  2:3374291/1‑140 (MQ=255)

                                            gATTGGAAATGGAATTCTCATTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCaaaa                                                                                             <  2:6084378/140‑1 (MQ=255)

                                             aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATTATTCGATCGTATGCAAGCCCTTCCCaaaaa                                                                                            >  2:6880611/1‑140 (MQ=255)

                                             aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGAtt                                                                                                                       <  2:1070372/113‑1 (MQ=255)

                                             aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAATCCCTTCCCaaaaa                                                                                            >  1:7014843/1‑140 (MQ=255)

                                             aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCaaaaa                                                                                            >  1:2903892/1‑140 (MQ=255)

                                             aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCaaaaa                                                                                            >  1:2188348/1‑140 (MQ=255)

                                             aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCaaaaa                                                                                            >  2:5089192/1‑140 (MQ=255)

                                             aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCaaaaa                                                                                            >  2:6404896/1‑140 (MQ=255)

                                             aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCaaaaa                                                                                            >  1:4187835/1‑140 (MQ=255)

                                             aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCaaaaa                                                                                            >  1:164589/1‑140 (MQ=255)

                                             aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCaaaaa                                                                                            >  2:3058802/1‑140 (MQ=255)

                                             aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCaaaaa                                                                                            >  2:5970425/1‑140 (MQ=255)

                                             aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCaaa                                                                                              >  1:7422118/1‑138 (MQ=255)

                                             aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCaaa                                                                                              <  2:2962456/138‑1 (MQ=255)

                                             aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTGCCcaacaa                                                                                            >  1:7260136/1‑140 (MQ=255)

                                             aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTAGATCGTATTCAAGCCCTTCCCaaaaa                                                                                            >  1:6708329/1‑140 (MQ=255)

                                             aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTAACTGTATCATGATTCGATCGTATGCAAGCCCATCCCaaaaa                                                                                            >  1:330571/1‑140 (MQ=255)

                                               tGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATTCAAGCCCTTCCCAAAAAC‑g                                                                                         >  2:6642494/1‑140 (MQ=255)

                                               tGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAAC‑g                                                                                         >  1:4329361/1‑140 (MQ=255)

                                               tGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTAACTGTATCATGATTCGATCGTATGCAAGACCTTACCAAAAAC‑g                                                                                         >  1:3795313/1‑140 (MQ=255)

                                                       gAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAAC‑GATAAAACg                                                                                 >  2:6965303/1‑140 (MQ=255)

                                                       gAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAAC‑GATAAAACg                                                                                 >  2:6001231/1‑140 (MQ=255)

                                                         aTTCTATTTTCGATATTTTTAACACCTTGTAAGAATAACCTTAAATTTACTCGGACAGTTTTTCTAAAAATCAACCAGTCTTTATTACCTTTATCATGATTCGATCGTATGAAAGCCCTCCCCAAAAAC‑CATAAAACGAc                                                                               <  2:4549230/140‑1 (MQ=255)

                                                               ttttCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAAC‑GATAAAACGACTCTGct                                                                         >  1:5945086/1‑140 (MQ=255)

                                                                 ttCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTGAGTACCTGTATCATGATTGGATCGTATGCAAGCCCTTCCCAAAAAC‑GATAAAACGACTCTGcgtc                                                                       >  1:870571/1‑140 (MQ=255)

                                                                      ttgTTTTTTCACGTTGTACCAATAACCTTAAAAGTACTCGCACAGTGATTATATAAATCAACCAGTGGTTTTTCCGTGTATAATGTGTCGCTCGTCTGCAAGCCGTCCCAAAAAAC‑GATAACACGACTCTGCTTCTTCaa                                                                  <  2:2408601/138‑1 (MQ=255)

                                                                         ttttATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGccc                                                                                                      >  1:6403323/1‑102 (MQ=255)

                                                                             aTCCCCTTGTAACAATAACAGACAAAGTACGCGGACAGGTCTTATATAACTCAACCAGTCGTTATTACCTGTAAATTGATTCGATCGTTTCCACGCCCGTCCAAAAAAC‑TAAAAAACGACTCTGCTTCTTCAAGTTCGCa                                                           <  2:2558344/140‑1 (MQ=255)

                                                                                            tAACCTTCCAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAAC‑GATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACAAaaccacgtc                                            >  2:3955675/1‑137 (MQ=255)

                                                                                            tAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTTTGCAAGCCCGTCCCAAAAAC‑GATAAAACGCCTCTGCTTCTTCAAGTTCGCAAGACATAACACCTtg                                            >  1:4505523/1‑140 (MQ=255)

                                                                                            tAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATAGTATGCAAGCCCTTCCCAAAAAC‑GATAAAACGACACTGCTTCTTCAAGTTCGCAAGACATAACAACTtg                                            >  2:357107/1‑140 (MQ=255)

                                                                                               ccTTCAATGTACTCGGACAGGTTTTCTATAAATGAACCAGTCTTTTTTACCTGTATATTTTTTCGATCGTATGCAAGCCCTACCAAAAAAC‑CAAAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtgatg                                         <  2:7493798/140‑1 (MQ=255)

                                                                                               ccTTAAATGAACGCGGCCAGGTTTTCTATAAATGAACCCGTGGTTTTTACCTGTATCATTATTGGATGGTTGCCACGCCCTGCCCAAAAAC‑GATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtgatg                                         <  2:4078463/140‑1 (MQ=255)

                                                                                               ccTTAAAATTACTCGCACAGTTCTTCTATAATTGAACCAGTGGTTTTTACCTTTAACTTTTTTCGCTGGTCGGAACGCCCTTCCAAAAACC‑GATAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtgatg                                         <  2:1318126/140‑1 (MQ=255)

                                                                                               ccTTAAAAGTACTCGTACCGTATTTATATAAATCACCCTGTGTTTTTTACTTGTATCTTTATTCTATCTTATGCACCCCCTCCCCAAAAAC‑GAAAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtgatg                                         <  2:5500620/140‑1 (MQ=255)

                                                                                               ccTACAAAGTACTCGCACCGTTTTTCTAAAAATCAACCAGTCGTTTTAACCTTTATATTTATCCGAACGAATCCACCCCCAACCAAAAACC‑CAAAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtgatg                                         <  1:5217119/140‑1 (MQ=255)

                                                                                               cattCAAAGTACTCGCCCCGTTGTTCTATAAATTAACCCTGCGTTATTCCGTGTATCTTGTTTCGCGCGTAGGCAACCCCTCCCCAAAACC‑TATAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtgatg                                         <  2:2129398/138‑1 (MQ=255)

                                                                                                    aaaGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCCAGCCCTTCCCAAAAAC‑GATAAAACGACTCGGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCt                                    >  1:4014024/1‑140 (MQ=255)

                                                                                                          cTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAAC‑GATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAagag                              >  1:199233/1‑140 (MQ=255)

                                                                                                          cTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAAC‑GATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAagag                              >  1:356463/1‑140 (MQ=255)

                                                                                                          cTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAAC‑GATAAAACGACTCTGCGTCTTCAAGTTCGCAAGACATAACAACGTGATGAGGCTTAagag                              >  1:7097757/1‑140 (MQ=255)

                                                                                                          cTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAAA‑GATAAAACGACTCTGCTTCTTCAACTTCGCAAGACATAACAACTTGATGAGGCTTAagaa                              >  2:4354143/1‑139 (MQ=255)

                                                                                                                  ggTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAACAAC‑GATAAAAAGACTCTGCTTCTTCAAGTTCGCCAGACATAACAACATGATGAGGCTTAAGAGCGATCATg                      >  1:4952956/1‑140 (MQ=255)

                                                                                                                  ggTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCATCCCAAAAAC‑GATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACAAAAAAACATGATGAGGCATAAGAGCGATCATg                      >  1:6897231/1‑140 (MQ=255)

                                                                                                                  ggTCTTCTATAAATGAACCAGTCGTTATTACCAGTATCATGATTCGATCGTATGCAACCCCATCCCCAAAAC‑GATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATg                      >  1:3364048/1‑140 (MQ=255)

                                                                                                                  ggTCTTCTATAAATGAACCAGTCGTTATTACCAGTATCAATATTCGATAGTATGCAAGCCCTTCCCAAAAAC‑CAAAAAACGACTCTGCTTATTCAATTTCGCAAAAAAAAACAAATTTAAGATGCTTAAGAGCGATCATg                      >  1:5698876/1‑140 (MQ=255)

                                                                                                                       tctATAATTGAACCCTGCGTTATTCCCTGTATCATTATGCGTGCGTTTGCAAGCCATTCCCAAACAC‑GATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTaa                 <  2:1480357/140‑1 (MQ=255)

                                                                                                                       tctATAAATTAACCAGTGTTTTTTACCTGTAAAATTATTCTATGTTTTCCAAGCCCTCCCAAAAAAC‑TATAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTaa                 <  2:2511194/140‑1 (MQ=255)

                                                                                                                             aaTGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAAC‑GATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGcc           >  2:676812/1‑140 (MQ=255)

                                                                                                                              aTGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAAC‑GCTAAAACGACTCTGCTTCTTCAAGTTCGCACGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCg          >  2:5912108/1‑140 (MQ=255)

                                                                                                                              aTGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAAC‑GATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTCGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCg          >  1:2817355/1‑140 (MQ=255)

                                                                                                                                cacacAGTCTTTATTACCTTTATAATTTTTCTATCGTATGC‑ACCCCCTCCAAAAAACATAAAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGAt        <  1:3227360/136‑1 (MQ=255)

                                                                                                                                 aaCCCGTTGTTATTACGTTTATCTTGATTCGATCTTATGCAAGCCCTTCCCAAAAAC‑GATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATa       <  2:1608339/140‑1 (MQ=255)

                                                                                                                                 aaCCCGTCGTTTTTACCTGTATCATGTTTCGTTCGTCGGCAAGCCCTCCCAAAAACC‑CATAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATa       <  2:893149/140‑1 (MQ=255)

                                                                                                                                 aaCCAGTTTTTTTTACCTTTATAATGATTCGATCGTATCCACGCCCGCCCCAAAAAC‑CATACCACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATa       <  1:101234/140‑1 (MQ=255)

                                                                                                                                 aaCCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAAC‑GATAAAACGACTCTGCTTCTGCAAGGTCGCAAGACATAACAACGTGATGAGGCTTAAGAGCGCTCATGTCTCAAGTGCCGaga       >  2:4447855/1‑138 (MQ=255)

                                                                                                                                   cctgTCTTTATTACCTGTATAATGATTCGATCTTAGGCAACCCCTTCCAAAAAAC‑TATAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACt     <  2:7179559/137‑1 (MQ=255)

                                                                                                                                   ccAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAAC‑GATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACt     >  2:5661899/1‑140 (MQ=255)

                                                                                                                                   ccAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAAC‑GATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACt     >  2:1720767/1‑140 (MQ=255)

                                                                                                                                   catgTCTTTATTACCTGTATAATTATTCGATCGTATGAACCCCCTCCCAAAAAAC‑GATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACt     <  2:7280426/137‑1 (MQ=255)

GAAAGAGAAGTCCTGCAATGTCCCAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCCCAAAAAC‑GATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCT  >  NZ_CP034943/3851083‑3851356

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`