BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	3,851,237	G→T	9.0%	I46I (ATC→ATA)	EO946_RS19820 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	3,851,237	0	G	T	9.0%	1234.2 / 24.0	553	I46I (ATC→ATA)	EO946_RS19820	hypothetical protein
Reads supporting (aligned to +/- strand): ref base G (368/108); new base T (16/60); total (385/168)
Fisher's exact test for biased strand distribution p-value = 3.36e-21
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 559 total aligned reads displayed.

CAATGTCCCAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCA‑AGCCCTTCCCAAAAAC‑GATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATAA  >  NZ_CP034943/3851098‑3851376

cAATGTCCCAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACAAGTCGTTATTAACTGTATCATg                                                                                                                                               >  2:4083340/1‑140 (MQ=255)

   tGTCCCAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGAtt                                                                                                                                            <  2:3793770/140‑1 (MQ=255)

   tGTCCCAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGAtt                                                                                                                                            <  1:675031/140‑1 (MQ=255)

   tGTCCCAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGAtt                                                                                                                                            <  2:6036853/140‑1 (MQ=255)

     tCCCAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCg                                                                                                                                          >  1:7220259/1‑140 (MQ=255)

     tCCCAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCg                                                                                                                                          >  1:5657472/1‑140 (MQ=255)

     tCCCAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCg                                                                                                                                          >  1:3660576/1‑140 (MQ=255)

        cAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATc                                                                                                                                       <  2:3260219/140‑1 (MQ=255)

        cAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATc                                                                                                                                       <  1:493107/140‑1 (MQ=255)

        cAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGGATCATGATTCGATc                                                                                                                                       <  2:5278740/140‑1 (MQ=255)

         aaTCTGCTCTCTGTAATATGGATTTGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCg                                                                                                                                      <  2:619881/140‑1 (MQ=255)

         aaTCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCg                                                                                                                                      <  1:1109063/140‑1 (MQ=255)

               ctctctGTAATATGGATTTGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGAAATCGGACATGTCATCTATAAATGAACAAGTCGTTAGTACCTGAAAAATGATACCAGCGTATgaa                                                                                                                                >  1:2167200/1‑138 (MQ=255)

               ctctctGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCa                                                                                                                                >  1:2105539/1‑140 (MQ=255)

               ctctctGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCa                                                                                                                                >  1:2711609/1‑140 (MQ=255)

               ctctctGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGAACGTATGCa                                                                                                                                >  1:1780443/1‑140 (MQ=255)

               ctctctGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACATTCAAAGTACACGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGAATAGATAGTATTCa                                                                                                                                >  1:2798410/1‑140 (MQ=255)

               ctctctGTAATATGGATTCGAAATGGAATTCTCTTTTCGCTATTTTTATCACGTTGTAAGCATAATATTAGAAGTACTAGGACTGGTCTTATATAAATGAAACAGTCGTTATTAATTGTATCTTGTTTGGGTCGTTTgat                                                                                                                                >  1:1381436/1‑138 (MQ=255)

               ctctctGTAATATGGATAGGAAATGGAATTCACTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTCTAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCa                                                                                                                                >  2:4261068/1‑140 (MQ=255)

                 ctctGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATTCA‑Ag                                                                                                                             >  1:4267509/1‑140 (MQ=255)

                    tGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATAACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTAAGCA‑AGccc                                                                                                                          >  1:287101/1‑140 (MQ=255)

                     gTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCA‑AGCCCt                                                                                                                         >  1:4703549/1‑140 (MQ=255)

                         tatGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCA‑AGCCCTTccc                                                                                                                     >  2:7710513/1‑140 (MQ=255)

                         tatGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACATGTATCATGATTAGATCGTATGAA‑AGCCCTTacc                                                                                                                     >  2:4491921/1‑140 (MQ=255)

                            ggATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCA‑AGCCCTTCCCaaa                                                                                                                  >  2:1869417/1‑140 (MQ=255)

                            ggATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCA‑AGCCCTTCCCaaa                                                                                                                  >  1:1690563/1‑140 (MQ=255)

                              aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGTTGCGATCGTATGCA‑AGCCCTTCCCaaaaa                                                                                                                >  2:5346069/1‑140 (MQ=255)

                              aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCA‑AGCCCTTCCCaaaaa                                                                                                                >  1:4187835/1‑140 (MQ=255)

                              aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCA‑AGCCCTTCCCaaaaa                                                                                                                >  1:7396197/1‑140 (MQ=255)

                              aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCA‑AGCCCTTCCCaaaaa                                                                                                                >  1:7495814/1‑140 (MQ=255)

                              aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCA‑AGCCCTTCCCaaaaa                                                                                                                >  1:1579873/1‑140 (MQ=255)

                              aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCA‑AGCCCTTCCCaaaaa                                                                                                                >  2:3174899/1‑140 (MQ=255)

                              aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCA‑AGCCCTTCCCaaaaa                                                                                                                >  1:2511194/1‑140 (MQ=255)

                              aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATAATGATTCGATCGTATGCA‑AGCCCTTCCCaaaaa                                                                                                                >  2:2485608/1‑140 (MQ=255)

                              aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCAGTATCATGATTCGATCGTATTCA‑ACCCCTTACCaaaaa                                                                                                                >  2:4736869/1‑140 (MQ=255)

                              aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTAACTGTATCATGATTCGATCGTATGCA‑AGCCCTTCCCaaaaa                                                                                                                >  1:3058905/1‑140 (MQ=255)

                              aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTAACTGTATCATGATTCGATCGTATGCA‑AGCCCATCCCaaaaa                                                                                                                >  1:330571/1‑140 (MQ=255)

                              aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGGTAGTACCTGTATCATGATTCGATCGTATGCA‑AGCCCGTCCCaaaaa                                                                                                                >  1:544879/1‑140 (MQ=255)

                               ttGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCA‑AGCCCTTCCCAAAAAc                                                                                                               >  1:6841006/1‑140 (MQ=255)

                                tGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATc                                                                                                                                       <  1:5950688/116‑1 (MQ=255)

                                tGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATTCA‑AGCCCTTCCCAAAAAC‑g                                                                                                             >  2:5896421/1‑140 (MQ=255)

                                tGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCA‑AGCCCTTCCCAAAAAC‑g                                                                                                             >  2:3628252/1‑140 (MQ=255)

                                tGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACAAGTCGTTATTACCTGTATCATGATTCGATCGTATGCA‑AGCCCTTCCCaaaaaag                                                                                                              >  2:6793517/1‑138 (MQ=255)

                                  gAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCA‑AGCCCTTCCCAAAAAC‑GAt                                                                                                           >  2:3526176/1‑140 (MQ=255)

                                   aaaTGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCA‑AGCCCTTCCCAAAAAC‑GATa                                                                                                          >  2:150949/1‑140 (MQ=255)

                                        gAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCA‑AGCCCTTCCCAAAAAC‑GATAAAACg                                                                                                     >  2:6965303/1‑140 (MQ=255)

                                                        aGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCA‑AGCCCTTCCCAAAAAC‑GATAAAACGAATCTGCTTCTTCaat                                                                                     >  2:5075207/1‑139 (MQ=255)

                                                          ttttATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCA‑AGccc                                                                                                                          <  2:6403323/102‑1 (MQ=255)

                                                              aacACCTTGTATCTATAACCTAAAAATTACTGGCACAGGTATATTATTAATCAACGAGTGTTTTTTACCTTTTTCTTGTTTCGATGGT‑TGCACCGCCCTACCAACACAC‑GATACAACGACTCTGCTTCTTCAAGTTCGCa                                                                               <  2:6909370/138‑1 (MQ=255)

                                                                    ttGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCA‑AGCCCTTCCCAAAAAC‑GATAAAACGACTCTGCTTCTTAAAGTTCGCAAGACaa                                                                         >  2:4189083/1‑139 (MQ=255)

                                                                    ttGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCA‑AGCCCTTCCCAAAAAC‑GATAAAACGACTCTGCTTATTAAAGTTCGCAAGACAt                                                                         >  2:3334781/1‑140 (MQ=255)

                                                                    ttGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCA‑AGCCCTTCCCAAAAAC‑CATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACAt                                                                         >  1:6629005/1‑140 (MQ=255)

                                                                             tAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTTTGCA‑AGCCCGTCCCAAAAAC‑GATAAAACGCCTCTGCTTCTTCAAGTTCGCAAGACATAACACCTtg                                                                >  1:4505523/1‑140 (MQ=255)

                                                                             tAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCA‑AGCCCTTCCCAAAAAC‑GATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACttt                                                                >  1:5187589/1‑139 (MQ=255)

                                                                             tAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCA‑AGCCCTTCCCAAAAAC‑GATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtg                                                                >  2:5968135/1‑140 (MQ=255)

                                                                             tAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCA‑AGCCCTTCCCAAAAAC‑GATAAAAAGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtg                                                                >  1:2013688/1‑140 (MQ=255)

                                                                                cctaaaaaGTACGCGCACAGGTCTTATATAAATGACCCAGTGGTTTTTACCTGTATAATTTTGCGATCGTAGGAA‑AGCCCGTCCCAAAAAC‑GAAAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtgatg                                                             <  2:2711609/135‑1 (MQ=255)

                                                                                ccTTCAATGTCGTCGTAGATGTATTCAATAAAGTAACCCGTCTTTTTTACGTGTATGATTTTGCGTTCTTTTCAC‑AGCCCTTCCCAAAAAC‑GAAAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtgatg                                                             <  2:6708329/140‑1 (MQ=255)

                                                                                ccTTCAATGTACTCGGACAGGTTTTCTATAAATGAACCAGTCTTTTTTACCTGTATATTTTTTCGATCGTATGCA‑AGCCCTACCAAAAAAC‑CAAAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtgatg                                                             <  2:7493798/140‑1 (MQ=255)

                                                                                ccTTAAAAGTACTCGTACCGTATTTATATAAATCACCCTGTGTTTTTTACTTGTATCTTTATTCTATCTTATGCA‑CCCCCTCCCCAAAAAC‑GAAAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtgatg                                                             <  2:5500620/140‑1 (MQ=255)

                                                                                actaaaaaTTACTCGCAAAGGTCTTATATAACTCACCCTGTCTTTTTTACGTTTATCATGATTCGATCTTCTGCA‑CGCCCTTCCCAAAAAC‑GAAAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtgatg                                                             <  2:5981063/135‑1 (MQ=255)

                                                                                        gTACTCGGACAGGTCTACTATAATGGAACCACGCGTTATTACCTGTATCTTGATGCGAGCGTAGGCA‑AGCCCTTCCCAAAAAC‑GCAAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTaa                                                     <  2:4601528/140‑1 (MQ=255)

                                                                                           cTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCA‑AGCCCTTCCCAAAAAC‑GATAAAACGACTCTGCTTCTTCCAGTTCGCAAGACATAACAACTTGATGAGGCTTAagag                                                  >  1:782871/1‑140 (MQ=255)

                                                                                           cTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCA‑AGCCCGTCCCAAACAC‑GATAAAACGACTCTGCTTCTTCAAGTTGGCAAGACATAACAACTTGATTAGGCTTAatag                                                  >  1:5306144/1‑140 (MQ=255)

                                                                                               gACAGTTGTTCTATAAATTACCCCGTGTTTATTCCCTGTATCATTATTCGAGCGTATGAA‑AGCCCTTCCCAAAAAC‑TATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGAt                                              <  2:2188348/140‑1 (MQ=255)

                                                                                                   ggTCTTCTATAAATGAACCAGTCGTTATTACCAGTATCATGATTCGATCGTATGCA‑ACCCCATCCCCAAAAC‑GATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATg                                          >  1:3364048/1‑140 (MQ=255)

                                                                                                       ttctATAAATGAACCAGTCTTTATTACCTGTATCATGATTCTATCTTATGCA‑AGCCCTTCCAAAAAAC‑CAAAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTa                                      <  2:1274001/140‑1 (MQ=255)

                                                                                                        tctATAAATTAACCAGTCTTTATTAGATTTATCATGATTCTATCTTTGGCA‑AGCCCATCCAAAAAAC‑TATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTaa                                     <  2:3872233/140‑1 (MQ=255)

                                                                                                           ataCATGAACCCGTGGTTATTACCTGTATCTTTATTCTTTGTTCTCCA‑CCCCCTTCCCAAAAAC‑GATAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCAGGTCTAAAtt                                  <  2:870571/140‑1 (MQ=255)

                                                                                                             atattaaCCATTCGTTTTTACGTTTATCTTTTTTCGATCTTATGCA‑AGCCCTTCCCAAAAAC‑TATAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGc                                <  2:6266460/135‑1 (MQ=255)

                                                                                                             aaaTCACCCAGCCGTTATTACCTTTATAATTATTCTTTCGTATGAA‑AGCCCTCCACAAAACC‑GAAAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGc                                <  1:5373412/140‑1 (MQ=255)

                                                                                                              aaTGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCA‑AGCCCTTCCCAAAAAC‑GATAAAACGACACTGCTTAATGAAGTTCGCAAGAAATAACAACATGATGAGGCTTAAGAGCGATCATGTCTAAATTGcc                               >  2:2552709/1‑140 (MQ=255)

                                                                                                               aTGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCA‑AGCCCTTCCCAAAAAC‑GATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCg                              >  1:5794743/1‑140 (MQ=255)

                                                                                                               aTGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCA‑AGCCCTTACCAAAAAC‑GATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACAAAAAAACTAGATGAGGCTTAAGAGCGATCATGTCTAAAATGCCg                              >  1:623454/1‑140 (MQ=255)

                                                                                                                 cacacAGTCTTTATTACCTTTATAATTTTTCTATCGTATGC‑‑ACCCCCTCCAAAAAACATAAAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGAt                            <  1:3227360/136‑1 (MQ=255)

                                                                                                                  aaCCCGTTGTTATTACGTTTATCTTGATTCGATCTTATGCA‑AGCCCTTCCCAAAAAC‑GATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATa                           <  2:1608339/140‑1 (MQ=255)

                                                                                                                  aaCCATTCTTTTTTACTTTTATCATGTTTCGATCGTATCCA‑AGCCCTTCCAAAAAAC‑TATAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATa                           <  2:4926989/140‑1 (MQ=255)

                                                                                                                  aaCCATTCTTTTTTACCTTAATCATTATTCGATTGTTTCCA‑AGCCCTTCCAAAAAAC‑TATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATa                           <  2:7361788/140‑1 (MQ=255)

                                                                                                                  aaCCAGTTTTTTTTACCTTTATAATGATTCGATCGTATCCA‑CGCCCGCCCCAAAAAC‑CATACCACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATa                           <  1:101234/140‑1 (MQ=255)

                                                                                                                  aaCCAGTGTTTTTTACCTTTATCTTGTTTCGATCTTATGCA‑AGCCCTTCACAAAAAC‑GATAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATa                           <  2:4394148/140‑1 (MQ=255)

                                                                                                                  aaCCAGTCTTTATTACGTTTATATTTATTCCATCGCATGCA‑CGCCCTTCCAAAAAAC‑GATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATa                           <  2:5726021/140‑1 (MQ=255)

                                                                                                                  aaCCAGTCTTTATTACCTGTATCTTGATTCGATCGTCTGCA‑AGCCCTTCCCAAAAAC‑GATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATa                           <  2:719071/140‑1 (MQ=255)

                                                                                                                  aaCCAGTCTTTATTACCTGTATCATGATTCGATCGTATGAA‑AGCCCTTCCCAAAAAC‑GATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATa                           <  2:1038719/140‑1 (MQ=255)

                                                                                                                  aaCCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCA‑AGCCCTTCCCAAAAAC‑GATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATa                           >  2:5809579/1‑140 (MQ=255)

                                                                                                                   aCCAGTCGTTATTACCTGTATCATGATTAGATCGTATGCA‑ACCCCTTCCCAAAAAC‑GATAAAACCACTCAGCTTCTTCAAGTTAGCAAGACATAACAACTTTATGAGGCTTAATAGCGATCATGTCTAAATTGCCGATaa                          >  1:7031745/1‑139 (MQ=255)

                                                                                                                   aCCAGTCGTTATTAACAGTATCAAGATTCGATCGTATGCA‑AGCCCTTCCCAAAAAA‑GATAAAACGACTATGCTTCTTCAAATTCGCAAGAAATAACAACATGATGAGGCTTAAGATCTATCATGTCAAAATTTCCGATaa                          >  1:3788580/1‑139 (MQ=255)

                                                                                                                    ccAGTCGTTATTACCTGTATCATGATTCGATCGTATGCA‑AGCCCTTCCCAAAAAC‑GATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACt                         >  2:1720767/1‑140 (MQ=255)

                                                                                                                    ccAGTCGTTATTACCTGTATCATGATTCGATCGTATGCA‑AGCCCTTCCCAAAAAC‑GATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACt                         >  2:1989487/1‑140 (MQ=255)

                                                                                                                       gTCGTTATTACCTGTATCATGATTCGATCGTATGCA‑AGCCCTTCCCAAAAAC‑GATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCt                      >  2:6552612/1‑140 (MQ=255)

                                                                                                                       gTCGTTATTACCTGTATCATGATTCGATCGTATGCA‑AGCCCTTCCCAAAAAC‑GATAAAACGACTCTGCTTCTTCAAGTTCGCAACACATAACAACTTGATGAGGCTTAAGAGCGATCATGGCTAAATTGCCGATACAGCt                      >  2:2970742/1‑140 (MQ=255)

                                                                                                                            tattaCCTGTATCTTGATTCGATCTTATGCA‑CGCCCTGCCCAAAAAC‑GATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAg                 <  2:286124/140‑1 (MQ=255)

                                                                                                                             attaCCTGTATCATGATTCGATCGTATGCA‑AGCCCTTCCCAAAAAC‑GATAAAACGACTCTGCTTCTTAAAGTTCGCAAGACATAACAACTTGATTAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGc                >  2:1433666/1‑140 (MQ=255)

                                                                                                                                   tGTATAATTATTCGATCGTCTGAA‑ACCCCTCCCAAAAAAC‑CAAAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTAt          <  2:1143379/140‑1 (MQ=255)

                                                                                                                                    gTATCATGATTCGATCGTATGCA‑AGCCCTTCCCAAAAAC‑GATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTAtt         >  2:4359797/1‑140 (MQ=255)

                                                                                                                                    gTATCATGATTCGATCGTATGCA‑AGCCCTTCCCAAAAAC‑GATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTAtt         >  2:762482/1‑140 (MQ=255)

                                                                                                                                    gTATCATGATTCGATCGTATGCA‑AGCCCTTCCCAAAAAC‑GATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTAtt         >  1:3538830/1‑140 (MQ=255)

                                                                                                                                     tATCATGATTCGATCGTATGCA‑AGCCCTTCCCAAAAAC‑GATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAAt                                   >  1:5061007/1‑113 (MQ=255)

                                                                                                                                     tATCATGATTCGATCGTATGCA‑AGCCCTTCCCAAAAAC‑GATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTAttt        >  1:6360895/1‑140 (MQ=255)

                                                                                                                                     tATCATGATTCGATCGTATGCA‑AGCCCTTCCCAAAAAC‑GATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTAttt        >  1:6965593/1‑140 (MQ=255)

                                                                                                                                      aTCATGATTCGATCGTATGCA‑AGCCCTTCCCAAAAAC‑GATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTc       >  1:4013476/1‑140 (MQ=255)

CAATGTCCCAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCA‑AGCCCTTCCCAAAAAC‑GATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATTTCCATAA  >  NZ_CP034943/3851098‑3851376

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`