BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	886,754	A→C	8.3%	V45G (GTT→GGT)	EO946_RS04490 ←	MFS transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	886,754	0	A	C	8.3%	715.4 / 4.2	312	V45G (GTT→GGT)	EO946_RS04490	MFS transporter
Reads supporting (aligned to +/- strand): ref base A (175/93); new base C (2/41); total (177/135)
Fisher's exact test for biased strand distribution p-value = 6.77e-15
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 325 total aligned reads displayed.

ACCAAGTCCGAACAGGCAAACCTCAATGAGAAAAAGTTTCTTTCTGCCGTAGCGGTCGGAAAGCTTTCCGACGATCGGCACACTGACGCTAAGGCCGAGCGTATAGAGGGTAATGCCCCATGACCCCCAGGAAGGAGAAAC‑GGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCTTTTCTGCTCTGTCAATGCTTCAGACATAT  >  NZ_CP034943/886615‑886889

aCCAAGTCCGAACAGGCAAACCTCAATGAGAAAAAGTTTCTTTCTGCCGTAGCGGTCGGAAAGCTTTCCGACGATCGGCACACTGACGCTAAGGCCGAGCGTATAGAGGGTAATGCCCCATGACCCCCAGGAAGGAGaaa                                                                                                                                          <  2:345599/140‑1 (MQ=255)

aCCAAGTCCGAACACGCAAACATCCATCAGAAAAACTTTCTTTCGGGCGTAGCGGTCGGAAAGCTTTGCGACGATCGGCACACTGACGCTAAGGCCGAGCGTATAGAGGGTACTGCCCCATGACACCTAGGAAGGAGaaa                                                                                                                                          <  2:4975473/140‑1 (MQ=255)

         gAACAGGCAAACCTCAATGAGAAAAAGTTTCTTTCTGCCGTAGCGGTCGGAAAGCTTTCCGACGATCGGCACACTGACGCTAAGGCCGAGCGTATAGAGGGTAATGCCCCATGACCCCCAGGAAGGAGAAAC‑GGCAAACg                                                                                                                                >  2:7217369/1‑140 (MQ=255)

         gAACAGGCAAACCTCAATGAGAAAAAGTTTATTTCTGACGTAGCGGTCGGAAAGCTTTCCGACGATAGGCACACTGACGGTAAGGCCGAGCGTAGAGAGGGTAATGCCCCATGACACCCAGGAAGGAGAAAC‑GGCAAACg                                                                                                                                >  1:58951/1‑140 (MQ=255)

          accAGCCAACCCTCAATGAGAAAATGTTTCTTTCTGCGGTTGCGGTCGGAAAGCTTTCCGACGATGGGCACACTGACGCTAAGGCCGAGCGTATAGTGGGTATTGCCCAATGACCCCCAGGAAGGACAAAC‑GGCAAACGa                                                                                                                               <  2:245147/138‑1 (MQ=255)

               gccaaCCTCCATGAGAAAAAGTTTCTTTCTGCCGTAGCGGTCGGAAAGCTTTCCGACGATCGGCACACTGACGCTCAGGCCGAGCGTATAGAGGGTAATTCCCCATGAACCCCAGGAAGGAGAAAC‑GGCAAACGACTCGt                                                                                                                          >  2:1497780/4‑140 (MQ=255)

                              aaaaaGTTTGTTTGTGCCGTAGGGGTCGTAAAGCTTTCCGACGATCGGCACACTGACGATACGGCCGAGGTTTTTGAGGGTAATGCCCAATGACCCCCAGGAAGCAGAACC‑GGCAAACGACTCGTTTATCGTTGTTAAag                                                                                                           <  2:4375230/140‑1 (MQ=255)

                              aaaaaGTTTCTTTCTGCTTTCGCGGTCGGAAAGCTTTCCGACGATCGCCACAATGACGCTAAGGCCGAGCGTATAGAGGGTACTCCCCCATCACCCCACGGACGGAGAACC‑GCCAAACGACTCGTTTATCGTTGTTAAag                                                                                                           <  2:7317519/140‑1 (MQ=255)

                              aaaaaGTTTCTTTCTGCCGTAGCGGTCGGAAAGCTTTCCGACGATCGGCACACTGACGCTAAGGCCGAGCGTATAGAGGGTAATGCCCCATGACCCCCAGGAAGGAGAAAC‑GGCAAACGACTCGTTTATCGTTGTTAAag                                                                                                           >  2:1133313/1‑140 (MQ=255)

                               aaaaTTTTTTTTCTGCCTTCGCGGGCTCAAAGTTTTCCCACTATCGGCACACTGACGCTACGGCCGAGCGTATCTCGGGTAATGCCCCATGACCCCCCGCAAGGAGAAAG‑GGCAAACGACTCGTTTATCGTTGTTAAagc                                                                                                          <  2:2227351/140‑1 (MQ=255)

                                      tctttctGCCGTAGCGGTCGGAAAGCTTTCCGACGATCGGCACACTGACGCTAAGGCCGAGCGTAAAGAGGGTAATGCCCCATGACCCCCAGGACGGACAAAC‑GGCAAACGACTCGTTTATCGTTTGTAAAGCAGCAGaa                                                                                                   >  2:2081070/1‑140 (MQ=255)

                                          tctGCGGTCGGGTTCGAAAAGCTGCCCCACTATCGGCACACTGCCCCTACGGCCGAGGTTATTGAGGGTAATGCCCCATGACCCCCAGGAAGGACAACC‑GGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAaataa                                                                                               <  1:5295643/140‑1 (MQ=255)

                                          tctGCCGTAGGGGTCGGAAAGCTTTCCGACGATCGGCACACTGACGCTAAGGCCGAGCGTATAGAGGGTAATGCCCCATGACCCCCAGCACGGAGAAAC‑GGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAaataa                                                                                               <  2:6199032/140‑1 (MQ=255)

                                               tgtcggggTCGCAAAGCTTTCCGACTATCGGAACACTGACGCTACGGCCGCGGGAATAGCGGTTAATGCCCCATCCCCCCCAGTATGGACAAAC‑GCCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCg                                                                                          <  1:1993936/134‑1 (MQ=255)

                                                         gtaatgCTGTCCGACTATGGGCACACTGCCGCTAAGGCCGAGCTTTTAGAGGGTAATCCCCCATGACCCCCAGTAAGTAGAACG‑GGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAgcg                                                                                <  2:2645725/135‑1 (MQ=255)

                                                         ggAATGATTTCCCACGATCGGCACACGGACGCTACGGCCTAGCGTATAGAGGGTAATGCCCAATGACCCCCAGCAAGGCGAAAC‑GGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAgcg                                                                                <  1:6623493/140‑1 (MQ=255)

                                                         ggAACGCTTTCCGACGAGCGGCACACTGACGCTAAGGCCGAGCGTTTAGTGGTTAATGCCCCAGGACCCCATGGAAGGATAAAC‑GGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAgcg                                                                                <  2:3549145/140‑1 (MQ=255)

                                                         ggAAAGCTTTCCGACGATCGGCACACTGACGCTAAGGCCGCGGGTATAGAGGGTAAGGCCCAATGACCCGCAGTACGGAGAAAC‑GGAACACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAgcg                                                                                <  2:1309220/140‑1 (MQ=255)

                                                           atcgCTTCCCGACGATCGGCACACTGCCGCTAAGGCCGAGCGTATAGTGGTTAAGCCCCCATGACCCCCAGGACGGACAACC‑GCCACACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCa                                                                              <  1:2808350/137‑1 (MQ=255)

                                                             cgttttCGGCCGATCGGCACCCGGACGCTACGGCCGCGCTTATAGAGGGTAATCCCCCATGACCCCCCGTCAGGAGAACC‑GGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGc                                                                            <  2:7764679/137‑1 (MQ=255)

                                                             agatttCCGACGATGGGCACACTGCGGCTAAGGCCCAGCGTATAGTGGGTAATGCCCCATTCCCCCCATGAAGCACAAAC‑GGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGc                                                                            <  2:5260159/137‑1 (MQ=255)

                                                              gtttgccGACGATCGGCACCCTCACGCTAAGGCCGTGCTTATAGCGGGTATTGCCCCATGACCCCCAGGAAGGAGAACG‑GGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGcc                                                                           <  1:5799657/135‑1 (MQ=255)

                                                              ggttgcggacgtTCGGAACACTGACGCTACGGCGGTGCGTTTATTGGGTAATGCCCCATGCCCCCCAGCACGGAGAACC‑GGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGcc                                                                           <  1:381665/133‑1 (MQ=255)

                                                                 ttCCGACGATCGGCACACTGACGCTAAGGCCGAGCTTATAGAGGGTAATGCCCCATGACCCCCAGGAAGGAGAAAC‑GGAAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATa                                                                        <  2:4650256/140‑1 (MQ=255)

                                                                  cccGACGATCGGCACAAGGACGCTACGGCCGTGCGTATAGTGGTTAAGGCCCCATCACCCCCAGGAAGGAGACCC‑GGAAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATaa                                                                       <  1:1406861/139‑1 (MQ=255)

                                                                   cctaggaTCGCCACACTGACGCTACGGCCGAGCGTATAGAGGTTAATGCCCCATCACCCCCAGGAAGGAGAAAC‑GGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATaaa                                                                      <  2:6475876/135‑1 (MQ=255)

                                                                   ccGTCGATGGTCACACTTACGATACGGCCTTGCTTATATAGGGAATTGCCCCTTCACCCCCAGGTAGGACAAAC‑GTCAAACGACTCGTTTATAGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATaaa                                                                      <  2:5652302/140‑1 (MQ=255)

                                                                   ccGACGATCGGCACACTGACCCTAAGGCCTAGCTTATAGAGGTTAAGGCCCAATCACCCCCAGTAAGCACAACC‑GGAAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATaaa                                                                      <  2:2565293/140‑1 (MQ=255)

                                                                        gctCGCCACACTGACGCTACGGCCGAGCGTATCGTGGGTAAGGCCCCAGCACCCCCAGGACGGAGAAAG‑GCCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAacacac                                                                 <  2:5090982/138‑1 (MQ=255)

                                                                        gaTCGGCACACTCCCCCCACGCCCGAGCGTATCGCGGTTAAGCCCCCAGCCCCCCCCGCATGCACAAAC‑GGCACACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAacacac                                                                 <  1:1003507/140‑1 (MQ=255)

                                                                        gaTCGCCACCCTGCCGCTACGGCCGAGCTTTGAGCGGGTAAGCCCCCATCCCCCACTGCACGCACAACC‑GGCACACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAacacac                                                                 <  1:155550/140‑1 (MQ=255)

                                                                         aTCGGCACACTGACGCTAAGGCCGAGCGTATAGAGGGTAATGCCCCATGACCCCCAGGAAGGAGAAAC‑GGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACAcc                                                                >  1:7607559/1‑140 (MQ=255)

                                                                         aTCGGCACACTGACGCTAAGGCCGAGCGTATAGAGGGTAATGCCCCATGACCCCCAGGAAGGAGAAAC‑GGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGGTATCCAGCGCAGCCATAAACACAcc                                                                >  1:97591/1‑140 (MQ=255)

                                                                           gggCACACTGCCGCCACGGCGGAGCTTATTGTGGGTTTTCCCCATTGCCCCCCCGGATGTCCAACC‑GGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCaa                                                              <  1:2562895/139‑1 (MQ=255)

                                                                            ggCACACTGAGGCTAAGGCCGAGCTTTTTGTGTGTAATGCCCATTGCCCCCCAGCAAGGTGAAAG‑GGCACACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAAt                                                             <  1:6473288/140‑1 (MQ=255)

                                                                                  cttcctatAAGCCCGAGCTTATAGAGGTTAATGCCCCATGACCCCCAGTATGTCTAAAC‑GGCACACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTaaa                                                       <  2:4491198/133‑1 (MQ=255)

                                                                                  cTGACTATAAGGCCGAGCTTATAGAGGTTAATGCCCAATGACCCCAAGTAAGGATAAAC‑GGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTaaa                                                       <  2:4217512/140‑1 (MQ=255)

                                                                                   tGACGCTAAGGCCGAGCGTATAGAGGGTAATGCCCCATGACCCCCAGGAAGGAGAAAC‑GGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAgcg                                                                                <  2:376700/114‑1 (MQ=255)

                                                                                   tGACGCTAAGGCCGAGCGTATAGAGGGTAATGCCCCATGACCCCCAGGAAGGAGAAAC‑GGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAAc                                                      >  2:7015758/1‑140 (MQ=255)

                                                                                    gACGCTAAGGCCGAGCGTATAGAGGGTAATGCCCCATGACCCCCAGGAAGGAGAAAC‑GGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACa                                                     >  1:3156270/1‑140 (MQ=255)

                                                                                    gACGCTAAGGCCGAGCGTATAGAGGGTAATGCCCCATGACCCCCAGGAAGGAGAAAC‑GGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACa                                                     >  1:3719491/1‑140 (MQ=255)

                                                                                        cTAAGGCCGAGCGTATAGAGGGTAATGCCCCATGACCCCCAGGAAGGAGAAAC‑GGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTCAACAGGCt                                                 >  2:6771342/1‑140 (MQ=255)

                                                                                        cTAAGGCCGAGCGTATAGAGGGTAATGCCCCATGACCCCCAGGAAGGAGAAAC‑GGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCt                                                 >  1:5518525/1‑140 (MQ=255)

                                                                                        cTAAGGCCGAGCGTATAGAGGGTAATGCCCCATGACCCCCAGGAAGGAGAAAC‑GGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCt                                                 >  1:1195334/1‑140 (MQ=255)

                                                                                        cTAAGGCCGAGCGTATAGAGGGTAATGCCCCATGACCCCCAGGAAGGAGAAAC‑GGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCt                                                 >  2:4818148/1‑140 (MQ=255)

                                                                                           aGGCCGAGCGTATAGAGGGTAATGCCCCATGACCCCCAGGAAGGAGAAAC‑GGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGAt                                              >  1:4011705/1‑140 (MQ=255)

                                                                                           aGGCCGAGCGTATAGAGGGTAATGCCCCATGACCCCCAGGAAGGAGAAAC‑GGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGAt                                              >  1:6246431/1‑140 (MQ=255)

                                                                                               cgtgcttattGAGGGTATTGCCCCCTTCCGCCCTGGAAGCATCATC‑GGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTGCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGcc                                          <  1:6900876/134‑1 (MQ=255)

                                                                                                 aGCGTATAGAGGGTAATGCCCCATGACCCCCAGGAAGGAGAAAC‑GGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCa                                        >  1:6606545/1‑140 (MQ=255)

                                                                                                       taGAGGGTAATGCCCCATGACCCCCAGGAAGGAGAAAC‑GGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTg                                  >  2:311132/1‑140 (MQ=255)

                                                                                                           gggTAATGCCCCATGACCCCCAGGAAGGAGAAAC‑GGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCtt                              >  1:186541/1‑140 (MQ=255)

                                                                                                            ggTAATGCCCCATGACCCCCAGGAAGGAGAAAC‑GGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAacacac                                                                 >  1:6047146/1‑104 (MQ=255)

                                                                                                            ggTAATGCCCCATGACCCCCAGGAAGGAGAAAC‑GGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCttt                             >  1:4192857/1‑140 (MQ=255)

                                                                                                            ggTAATGCCCCATGACCCCCAGGAAGGAGAAAC‑GGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCttt                             >  1:4301135/1‑140 (MQ=255)

                                                                                                            ggTAATGCCCCATGACCCCCAGGAAGGAGAAAC‑GGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCttt                             >  2:5631932/1‑140 (MQ=255)

                                                                                                            ggTAATGCCCCATGACCCCCAGGAAGGAGAAAC‑GGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCttt                             >  2:5221973/1‑140 (MQ=255)

                                                                                                            ggTAATGCCCCATGACCCCCAGGAAGGAGAAAC‑GGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCttt                             >  1:1680259/1‑140 (MQ=255)

                                                                                                            ggTAATGCCCCATGACCCCCAGGAAGGAGAAAC‑GGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCttt                             >  2:4897950/1‑140 (MQ=255)

                                                                                                            ggTAATGCCCCATGACCCCCAGGAAGGAGAAAC‑GGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCttt                             >  1:3335298/1‑140 (MQ=255)

                                                                                                            ggTAATGCCCCATGACCCCCAGGAAGGAGAAAC‑GGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCttt                             >  2:4141167/1‑140 (MQ=255)

                                                                                                            ggTAATGCCCCATGACCCCCAGGAAGGAGAAAC‑GGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCttt                             >  2:392789/1‑140 (MQ=255)

                                                                                                            ggTAATGCCCCATGACCCCCAGGAAGGAGAAAC‑GGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCttt                             >  2:3408558/1‑140 (MQ=255)

                                                                                                            ggTAATGCCCCATGACCCCCAGGAAGGAGAAAC‑GGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCttt                             >  1:7390043/1‑140 (MQ=255)

                                                                                                            ggTAATGCCCCATGACCCCCAGGAAGGAGAAAC‑GGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCttt                             >  1:7219499/1‑140 (MQ=255)

                                                                                                            ggTAATGCCCCATGACCCCCAGGAAGGAGAAAC‑GGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCttt                             >  1:2911674/1‑140 (MQ=255)

                                                                                                            ggTAATGCCCCATGACCCCCAGGAAGGAGAAAC‑GGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCtgt                             >  1:7071576/1‑138 (MQ=255)

                                                                                                            ggTAATGCCCCATGACCCCCAGGAAGGAGAAAC‑GGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTGTTGGCttt                             >  1:4557291/1‑140 (MQ=255)

                                                                                                            ggTAATGCCCCATGACCCCCAGGAAGGAGAAAC‑GGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGGTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCttt                             >  2:4603715/1‑140 (MQ=255)

                                                                                                             gTAATGCCCCATGACCCCCATG‑ATGATAAACGGGAAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCtttt                            <  1:3207087/140‑1 (MQ=255)

                                                                                                             gTAAGGCCCAATGACCCCCAGGAAGGACAAAC‑GGCACACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCtttt                            <  1:7758926/140‑1 (MQ=255)

                                                                                                              tAATGCCCCATGACCCCCAGGACGGAGAAAC‑GGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTGTTGGCTTTTc                           >  2:6562058/1‑140 (MQ=255)

                                                                                                               aaTGCCCCATGACCCCCAGGAAGGAGAAAC‑GGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCTTTTCt                          >  2:4130369/1‑140 (MQ=255)

                                                                                                                aTGCCCCATTACCCCCAGGAAGGATAAAC‑GGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCTTTTCTg                         <  2:58951/140‑1 (MQ=255)

                                                                                                                aTGCCCCATGACCCCCAGGAAGGAGAAAC‑GGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTGTTGGCTTTTCTg                         >  1:4840617/1‑140 (MQ=255)

                                                                                                                 tGCCCCATGACCCCCAGGAAGGAGAAAC‑GGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCTTTTCTGc                        >  1:2296659/1‑140 (MQ=255)

                                                                                                                 ggCCCCATCCCCCCAAGCACGGACAACC‑GGAAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATCATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCTTTTCTGc                        <  1:3577380/139‑1 (MQ=255)

                                                                                                                  gCCCCATGACCCCCCGGAAGCAGAAAC‑GGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCTTTTCTGct                       <  2:1958814/140‑1 (MQ=255)

                                                                                                                   ccacaggACCCCCAGGACCTAGACAC‑GGCAAACGACTCCTTGATCGTTGTTAAAGCAGCAGAACTAATTCCCTGATCGAGCGCAGCCCTAAACACACCAATCGTACAAAGGCGGATCGCCCACTTTTGGCTTTTCGGctc                      <  2:7578698/134‑1 (MQ=255)

                                                                                                                    cccATGACCCCCAGGAAGGAGAAAC‑GGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCTTTTCTGctct                     >  1:3059701/1‑140 (MQ=255)

                                                                                                                    cccATGACCCCCAGGAAGGAGAAAC‑GGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCTTTTCTGctct                     >  1:4446427/1‑140 (MQ=255)

                                                                                                                      cATGACCCCCAGGAAGGAGAAAC‑GGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATaaa                                                                      >  1:6801504/1‑89 (MQ=255)

                                                                                                                      cATGACCCCCAGGAAGGAGAAAC‑GGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCTTTTCTGCTCTGt                   >  1:2503213/1‑140 (MQ=255)

                                                                                                                        tgccccccATGACTGAGAAAC‑GCCACACGAATCGTTTATCGTTGTTAAACCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCTTTTCTGCTCTGTCa                 <  2:2115007/137‑1 (MQ=255)

                                                                                                                        tGACCCCCAGGAAGGAGAAAC‑GGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCTTTTCTGCTCTGTCa                 >  2:7681867/1‑140 (MQ=255)

                                                                                                                        tGACCCCCAGGAAGGAGAAAC‑GGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCTTTTCTGCTCTGTCa                 >  1:5381661/1‑140 (MQ=255)

                                                                                                                        tGACCCCCAGGAAGGAGAAAC‑GGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCTTTTCTGCTCTGTCa                 >  2:3632117/1‑140 (MQ=255)

                                                                                                                        tGACCCCCAGCACGCAGAAAC‑GGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCTTTTCTGCTCTGTCa                 <  1:5099860/140‑1 (MQ=255)

                                                                                                                        tGACCCCCAGCAAGGACAAAC‑GCCACACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCTTTTCTGCTCTGTCa                 <  2:1680259/140‑1 (MQ=255)

                                                                                                                            ccccAGGAAGGAGACAC‑GGCAAACGACTCGTTTATCGTTGTTACAGCAGCAGAAATAATTCGGTTATCCAGCGCTGCCATACTCACTCCTCTCGTAAACCGGCTGTTCGCCCCCTTGTGGCTTTTCTGCTCTGTCAATGc             >  2:6802956/1‑140 (MQ=255)

                                                                                                                              ccAGGAAGGAGAAAC‑GGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCTTTTCTGCTCTGTCAATGCtt           >  2:4084855/1‑140 (MQ=255)

                                                                                                                              ccAGCAAGGAGAACG‑GCCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCTTTTCTGCTCTGTCAATGCtt           <  2:2891436/140‑1 (MQ=255)

                                                                                                                                    cgcacaaaC‑GGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCTTTTCTGCTCTGTCAATGCTTCAGACa     <  1:2262714/135‑1 (MQ=255)

                                                                                                                                    aggaCAACC‑GGCACACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCTTTTCTGCTCTGTCAATGCTTCAGACa     <  2:582339/140‑1 (MQ=255)

                                                                                                                                     ggaGAAAC‑GGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCTTTTCTGCTCTGTCAATGCTTCAGACat    >  1:470962/1‑140 (MQ=255)

                                                                                                                                      gagaAAC‑GGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCTTTTCTGCTCTGTCAATGCTTCAGACata   >  2:2390442/1‑140 (MQ=255)

                                                                                                                                      gagaAAC‑GGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCTTTTCTGCTCTGTCAATGCTTCAGACata   >  2:7413833/1‑140 (MQ=255)

                                                                                                                                      gagaAAC‑GGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCTTTTCTGCTCTGTCAATGCTTCAGACata   >  1:1747755/1‑140 (MQ=255)

                                                                                                                                      gagaAAC‑GGAAATCGAGTCGTTTATCGGTGTTAAACCATCAGTAATAATTCCGTTATCCAGCGCAGCCAGAAACAAACCAAACTTAAACAGTCTGATCGCCCACTTGTGGCTTTTCTGCTCTGTCAATGCTACAGACata   >  2:6014676/1‑140 (MQ=255)

                                                                                                                                       agaAAC‑GGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAacacac                                                                 >  2:6363525/1‑77 (MQ=255)

                                                                                                                                       agaAAC‑GGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAacacac                                                                 <  1:6363525/77‑1 (MQ=255)

ACCAAGTCCGAACAGGCAAACCTCAATGAGAAAAAGTTTCTTTCTGCCGTAGCGGTCGGAAAGCTTTCCGACGATCGGCACACTGACGCTAAGGCCGAGCGTATAGAGGGTAATGCCCCATGACCCCCAGGAAGGAGAAAC‑GGCAAACGACTCGTTTATCGTTGTTAAAGCAGCAGAAATAATTCCGTTATCCAGCGCAGCCATAAACACACCAATCGTAAACAGGCTGATCGCCCACTTTTGGCTTTTCTGCTCTGTCAATGCTTCAGACATAT  >  NZ_CP034943/886615‑886889

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`