BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	1,036,283	C→A	8.7%	S43Y (TCT→TAT)	EO946_RS05355 →	ABC transporter permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	1,036,283	0	C	A	8.7%	707.8 / 5.5	432	S43Y (TCT→TAT)	EO946_RS05355	ABC transporter permease
Reads supporting (aligned to +/- strand): ref base C (226/125); new base A (58/2); total (304/128)
Fisher's exact test for biased strand distribution p-value = 3.33e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 432 total aligned reads displayed.

GGGGGCCGTCTATGAATAAATTTTGGATCATGCTCTCTCATACATACAAAAATAAGATTATGGCTAAATCTTTTATTATTTCGACTGTCATCACAGTACTGCTTGTTTTAGTTGTAACGAATCTGGAGTCGATCATTTCTTTGTTTCAAGGTGATGATGCGAAAGAAAAAATCGC‑CGTTGTTGATGAAACAAACGAATTATATCCGATTTTCTCGAAGCAGCTGAAAGCGGTGGATACAAACGGTGATCTTGATGTGAAGCAATCAAAGCTATCGG  >  NZ_CP034943/1036145‑1036420

      cGTCTATGAATAAATTTTGGATCATGCTCTCTCATACATACAAAAATAAGATTATGGCTAAATCTTTTATTATTTCGACTGTCATCACAGTACTGCTTGTTTTAGTTGTAACGAATCTGGAGTCGATCATTTCTTTGttt                                                                                                                                     <  2:3387877/140‑1 (MQ=255)

        tCTATGAATAAATTTTGTATCATGCTCTATCATACATAAAAAAATAAGATTATGGCTAAATCTTTTATTATTTCGACTGTCATCACAGTACTGCTTGTTTTAGTTGTAACGAATCTGGAGTCGATCATTTCTTTGTTTCa                                                                                                                                   <  1:7221917/140‑1 (MQ=255)

        tCTATGAATAAATTTTGGATCATGCTCTCTCATACATACAAAAATAAGATTATGGCTAAATCTTTTATTATTTCGACTGTCATCACAGTACTGCTTGTTTTAGTTGTAACGAATCTGGAGTCGATCATTTATTTGTTTCa                                                                                                                                   >  1:2454712/1‑140 (MQ=255)

        tCTATGAATAAATTTTGGATCATGCTCTCTCATACATACAAAAATAAGATTATGGCTAAATCTTTTATTATTTCGACGGTCATCACAGTACTGCTTGTTTTAGTTGTAACGAATCTGGAGTCGATCATTTGTTTTTTTCa                                                                                                                                   >  1:1969078/1‑140 (MQ=255)

              aaaaaaTTTTGGATCATGCTCTCTCATACATACAAAAATAAGATTATGGCTAAATCTTTTATTATTTCGACTGTCATCACAGTACTGCTTGTTTTAGTTGTAACGAATCTGGAGTCGATCATTTCTTTGTTTCAAGGtga                                                                                                                             <  1:7379571/137‑1 (MQ=255)

              aaTAAATTTTGGATCATGCTCTCTCATACATACAAAAATAAGATTATGGCTAAATCTTTTATTATTTCGACTGTCATCACAGTACTGCTTGTTTTAGTTGTAACGAATCTGGAGTCGATCATTTCTTTGTTTCAAGGtga                                                                                                                             <  1:6076260/140‑1 (MQ=255)

              aaTAAATTTTGGATCATGATCTCTCATACATACAAAAATAAGATTATGGCTAAATCTTTTATTATTTCGACTGTCATCACAGTACTGCTTGTTTTAGTTGTAACGAATCTGGAGTCGATCATTTCTTTGTTTCAAGGtga                                                                                                                             <  1:5168007/140‑1 (MQ=255)

                        ggATCATGCTCTCTCATACATACAAAAATAAGATTATGGCTAAATCTTTTATTATTTCGACTGTCATCACAGTACTGCTTGTTTTAGTTGTAACGAATCTGGAGTCGATCATTTCTTTGTTTCAAGGTGATGATGCgaaa                                                                                                                   >  1:6160856/1‑140 (MQ=255)

                              tGCTCTCTCATACATACAAAAATAAGATTATGGCTAAATCTTTTATTATTTCGACTGTCATCACAGTACTGCTTGTTTTAGTTGTACCGAATCTGGAGTCGATCATTTCGATGTTTACAGGTGATGATGCGTCAGcaaaa                                                                                                             >  1:950359/1‑140 (MQ=255)

                                     tcATACATACAAAAATAAGATTATGGCTAAATCTTTTATTATTTCGACTGTCATCACAGTACTGCTTGTTTTAGTTGTAACGAATCTGGAGTCGATAATTTATTTGTTTAAATGTGTTGATGCGAAAGAAAAAATCGC‑Cg                                                                                                     >  1:5003029/1‑140 (MQ=255)

                                        tacatacaAAAATAAGATTATGGCTAAATCTTTTATTATTTCGACTGTCATCACAGTACTGCTTGTTTTAGTTGTAACGAATCTGGAGTCGATCATTTCTTTTTTTCAAGGTGATGATGCGAAAGAAAAAATAGC‑Cggtg                                                                                                  >  1:2163215/1‑140 (MQ=255)

                                        tacatacaAAAATAAGATTATGGCTAAATCTTTTATTATTTCGACTGTCATCACAGTACTGCTTGTTTTAGTTGTAACGAATCTGGAGTCGATCATTTCTTTGTTTGAAGGTGTTGATGCGAAAGAAAAAATCGC‑Cgggg                                                                                                  >  1:4784137/1‑137 (MQ=255)

                                        tacatacaAAAATAAGATTATGGCTAAATCTTTTATTATTTCGACTGTCATCACAGTACTGCTTGTTTTAGTTGTAACGAATCTGGAGTCGATCATTTCTTTGTTTCAAGGTGATGATGCGAAAGAAAAAATCGC‑Cgttg                                                                                                  >  2:7645179/1‑140 (MQ=255)

                                        tacatacaAAAATAAGATTATGGCTAAATCTTTTATTATTTCGACTGTCATCACAGTACTGCTTGTTTTAGTTGTAACGAATCTGGAGTCGATCATTTCTTTGTTTCAAGGTGATGATGCGAAAGAAAAAATCGC‑Cggtg                                                                                                  >  2:6740350/1‑140 (MQ=255)

                                        tacatacaAAAATAAGATTATGGCTAAATCTTTTATTATTTCGACTGTCATCACAGTACTGCTTGTTTTAGTTGTAACGAATCTGGAGTCGATCAATTCTTTGTGTCAAGGGGATTATGCGAAAGAAAAAAAAGC‑Cgtag                                                                                                  >  1:6909142/1‑138 (MQ=255)

                                             acaAAAATAAGATTATGGCTAAATCTTTTATTATTTCGACTGTCATCAGAGTACTGCTTGTTTTAGTTGTAACGAATCTGGAGTCGATCATTTCTTTGTTTGAAGGTGATGTTGCGAAAGAAAAAATCGC‑CGGTGTtgat                                                                                             >  1:3920577/1‑140 (MQ=255)

                                             acaAAAATAAGATTATGGCTAAATCTTTTATTATTTCGACTGTCATCACAGTACTGCTTGTTTTAGTTGTAACGAATCTGGAGTCGATCATTTCTTTGTTTCCAGGTGATGATGCGAAAGAAAAAATCGC‑CGTTGTtgat                                                                                             >  1:5746709/1‑140 (MQ=255)

                                             acaAAAATAAGATTATGGCTAAATCTTTTATTATTTCGACTGTCATCACAGTACTGCTTGTTTTAGTTGTAACGAATCTGGAGTCGATCATTTCTTTGTTTCAAGGTGATGATGCGAAAGAAAAAATCGC‑CGTTGTtgat                                                                                             >  2:6970324/1‑140 (MQ=255)

                                             acaAAAATAAGATTATGGCTAAATCTTTTATTATTTCGACTGTCATCACAGTACTGCTTGTTTTAGTTGTAACGAATCTGGAGTCGATCATTTCTTTGTTTCAAGGTGATGATGCGAAAGAAAAAATCGC‑CGTTGTtgat                                                                                             >  2:1860823/1‑140 (MQ=255)

                                             acaAAAATAAGATTATGGCTAAATCTTTTATTATTTCGACTGTCATCACAGTACTGCTTGTTTTAGTTGTAACGAATCTGGAGTCGATCATTTCTTTGTTTAAATGTGATGATGCGAAAGAAAAAATAGC‑CGTTGTtgat                                                                                             >  1:869077/1‑140 (MQ=255)

                                               aaaaaTAAGATTATGGCTAAATCTTTTATTATTTCGACTGTCATCCCAGTACTGCTTGTTTTAGTTGTAACGAATCTGGAGTCGATCATTTCTTTGTTTCAAGGTGATGATGCGAAAGAAAAAATAGC‑CGTTGTtgatga                                                                                           >  2:1544828/1‑140 (MQ=255)

                                               aaaaaTAAGATTATGGCTAAATCTTTTATTATTTCGACTGTCATCACAGTACTGCTTGTTTTAGTTGTAACGAATCTGGAGTCGATCCTTTCTTTGTTTAAAGGTGATGATGCGTAAGAAAAAAACGC‑CGGTGTtgatga                                                                                           >  2:4152198/1‑140 (MQ=255)

                                               aaaaaTAAGATTATGGCTAAATCTTTTATTATTTCGACTGTCATCACAGTACTGCTTGTTTTAGTTGTAACGAATCTGGAGTCGATCATTTGTTTGTTTCAAGGTGATGATGCGAAAGAAAAAATCGC‑CGTTGTtgatga                                                                                           >  1:75240/1‑140 (MQ=255)

                                               aaaaaTAAGATTATGGCTAAATCTTTTATTATTTCGACTGTCATCACAGTACTGCTTGTTTTAGTTGTAACGAATCTGGAGTCGATCATTTCTTTGTTTCAATGTGATGTTGCGAAAGAAAAAATCGG‑CGTTGGtgatga                                                                                           >  1:3481785/1‑140 (MQ=255)

                                               aaaaaTAAGATTATGGCTAAATCTTTTATTATTTCGACTGTCATCACAGTACTGCTTGTTTTAGTTGTAACGAATCTGGAGTCGATCATTTCTTTGTTTCAAGGTGATGATGCGAAAGAAAAAATCGC‑Cgtt                                                                                                   <  1:3688096/132‑1 (MQ=255)

                                               aaaaaTAAGATTATGGCTAAATCTTTTATTATTTCGACTGTCATCACAGTACTGCTTGTTTTAGTTGTAACGAATCTGGAGTCGATCATTTCTTTGTTTCAAGGTGATGATGCGAAAGAAAAAATCGC‑CGTTGTtgatga                                                                                           >  2:3741521/1‑140 (MQ=255)

                                               aaaaaTAAGATTATGGCTAAATCTTTTATTATTTCGACTGTCATCACAGTACTGCTTGTTTTAGTTGTAACGAATCTGGAGTCGATCATTTCTTTGTTTAAATGTGATGATGCGAAAGAAAAAATCGC‑CGTTGTtgatta                                                                                           >  2:6679469/1‑138 (MQ=255)

                                                 aaaTAAGATTATGGCTAAATCTTTTATTATTTCGACTGTCATCACAGTACTGCTTGGTTTAGTTGTAACGAATCTGGAGTCGATCATTTCTTTGTTTCCAGGGGATGATGCGACAGTAAAAATCGC‑CGTTGTTGATGaaa                                                                                         >  2:2940982/1‑140 (MQ=255)

                                                     aataTTATGGCTAAATCTTTTATTATTTCGACTGTCATCACAGTACTGCTTGTTTTAGTTGTAACGAATCTGGAGTCGATCATTTCTTTGTTTCAAGGTGATGATGCGAAAGAAAAAATCGC‑CGTTGTTGATGaaacaaa                                                                                     <  2:5483994/137‑1 (MQ=255)

                                                     aaGATTATGGCTAAATCTTTTATTATTTCGACTGTCATCACAGTACTGCTTGTTTTAGTTGTAACGAATCTGGAGTCGATCATTTCTTTGTTTCAAGGTGATGATGCGAAAGAAAAAATCGC‑CGTTGTTGATGaaacaaa                                                                                     <  1:5308744/140‑1 (MQ=255)

                                                         ttATGGCTAAATCTTTTATTATTTCGACTGTCATCACAGTACTGCTTGTTTTAGTTGTAACGAATCTGGAGTCGATCATTTGTTTGTTTCAAGGTGATGATGCGAAAGAAAAAATCGC‑CGTTGTTGATGAAACAAACGaa                                                                                 >  2:3996105/1‑140 (MQ=255)

                                                           aTGGCTAAATCTTTTATTATTTCGACTGTCATCACAGTACTGCTTGTTTTAGTTGTAACGAATCTGGAGTCGATGATTTATTTGTTTCAAGGTGATGATGCGAAAGTAAAAATAGC‑CGTTGTTGATGGAACAAACGAAtt                                                                               >  1:1330682/1‑140 (MQ=255)

                                                           aTGGCTAAATCTTTTATTATTTCGACTGTCATCACAGTACTGCTTGTTTTAGTTGTAACGAATCTGGAGTCGATCATTTCTTTGTTTGGAGGTGATGATGCGAAAGAAAAAATCGG‑CGTTGTTGTTGAAACAAACGAAtt                                                                               >  1:3355784/1‑140 (MQ=255)

                                                           aTGGCTAAATCTTTTATTATTTCGACTGTCATCACAGTACTGCTTGTTTTAGTTGTAACGAATCTGGAGTCGATCATTTCTTTGTTTCAAGGTGATGATGCGAAAGAAAAAATCGC‑CGTTGTTGATGAAACAAACGAAtt                                                                               >  2:3270702/1‑140 (MQ=255)

                                                           aTGGCTAAATCTTTTATTATTTCGACTGTCATCACAGTACTGCTTGTTTTAGTTGTAACGAATCTGGAGTCGATCATTTCTTTGTTTCAAGGTGATGATGCGAAAGAAAAAATCGC‑CGTTGTTGATGAAACAAACGAAtt                                                                               >  2:2749323/1‑140 (MQ=255)

                                                           aTGGCTAAATCTTTTATTATTTCGACTGTCATCACAGTACTGCTTGTTTTAGTTGTAACGAATCTGGAGTCGATCATTTCTTTGTTTCAAGGTGATGATGCGAAAGAAAAAATCGC‑CCGTGTTGATGAAACAAAAGAAtt                                                                               >  2:2693762/1‑140 (MQ=255)

                                                              gCTAAATCTTTTATTATTTCGACTGTCATCACAGTACTGCTTGTTTTAGTTGTAACGAATCTGGAGTCGATCATTTCTTTGTTTCAAGGTGATGATGCGAAAGAAAAAATCGC‑CGTTGTTGATGAAACAAACGAATtata                                                                            <  2:2577031/140‑1 (MQ=255)

                                                                  aaTCTTTTATTATTTCGACTGTCATCACAGTACTGCTTGTTTTAGTTGTAACGAATCTGGAGTCGATCATTTATTTGTTTCAAGGTGATGATGCGAAAGAAAAAATAGC‑CGTTGTTGATGAAACAAACGTATTATATACg                                                                        >  2:2172558/1‑140 (MQ=255)

                                                                        ttattaTTTCGACTGTCATCACAGTACTGCTTGTTTTAGTGGTAACGAATCTGGAGTCGATCATTTCTTTGTTTCAAGGTGAGGATGCGAAAGAAAAAATCGC‑CGTTGTTGATGAAACAAACGAATTAGTTCCGATTTtc                                                                  >  2:5317062/1‑140 (MQ=255)

                                                                              tttCGACTGTCATCACAGTACTGCTTGTTTTAGTTGTAACGAATCTGGAGTCGATCATTTCTTTGTTTCAAGGTGATGATGCGAAAGAAAAAATCGC‑CGTTGTTGATGAAACAAACGAATTATATCCGATTTTCTCGAag                                                            <  1:1459223/140‑1 (MQ=255)

                                                                              tttCGACTGTCATCACAGTACTGCTTGTTTTAGTTGTAACGAATCTGGAGTCGATCATTTCTTTGTTTCAAGGTGATGATGCGAAAGAAAAAATCGC‑CGTTGTTGATGAAACAAACGAATTATATCCGATTTTCTCGAag                                                            <  1:3190444/140‑1 (MQ=255)

                                                                              tttCGACTGTCATCACAGTACTGCTTGTTTTAGTTGTAACGAATCTGGAGTCGATCATTTCTTTGTTTCAAGGTGATGATGCGAAAGAAAAAATCGC‑CGTTGTTGATGAAACAAAAGAATTATATACGATTTTCTAGAag                                                            >  2:5170536/1‑140 (MQ=255)

                                                                              tttCGAATGTCATCACAGTACTTCTTTTTTTAGTTGTAACTAATCTGGAGTCGATCATTTCTTTGTTTCAAGGTGATGATGAGAAAGAAATAATCGC‑CGTTGTTGATGAAAAAAACGAATTATATCCGATTTTCTCGAag                                                            <  2:3828768/140‑1 (MQ=255)

                                                                                tCGACTGTCATCACAGTACTGCTTGTTTTAGTTGTAACGAATCTGGAGTCGATCATTTCTTTGTTTCAAGGTGATGATGCGAAAGAAAAAATCGC‑CGTTGTTGATTAAACAAAAGAATTATATACGATTTTCTCGAagca                                                          >  2:1934423/1‑140 (MQ=255)

                                                                                  gACTGTCATCACAGTACTGCTTGTTTTAGTTGTAACGAATCTGGAGTCGATCATTTCTTTGTTTCAAGGTGATGATGCGAAAGAAAAAATCGC‑CGTTGTTGATGAAACAAACGAATTATATCCGATTTTCTCGAagcagc                                                        <  1:2749323/140‑1 (MQ=255)

                                                                                       tcatcaCAGTACTGCTTGTTTTAGTTGTAACGAATCTGGAGTCGATCATTTCTTTGTTTCAAGGTGATGATGCGAAAGAAAAAATCGC‑CGTTGTTGATGAAACAAACGAATTATATCCGATTTTCTCGAAGCAGCTGaaa                                                   <  2:863503/140‑1 (MQ=255)

                                                                                       tcatcaCAGTACTGCTTGTTTTAGTTGTAACGAATCTGGAGTCGATCATTTCTTTGTTTCAAGGTGATGATGCGAAAGAAAAAATCGC‑CGTTGTTGATGAAACAAACGAATTATATCCGATTTTCTCGAAGCAGCTGaaa                                                   <  2:2412297/140‑1 (MQ=255)

                                                                                        catcaCAGTACTGCTTGTTTTAGTTGTAACGAATCTGGAGTCGATCATTTCTTTGTTTCAAGGTGATGTTGCGAAAGAAAAAAAAGC‑CGTTGTTGATTTAACAAACGAATTATATCCGATTTTCTCGAAGCAGCTGCAAg                                                  >  1:3557157/1‑140 (MQ=255)

                                                                                        catcaCAGTACTGCTTGTTTTAGTTGTAACGAATCTGGAGTCGATCATTTCTTTGTTTCAAGGTGATGATGCGAAAGAAAAAATCGC‑CGTTGTTGATGAAAAAAACGAATTATATCCGATTTTCTCGAAGCAGCTGAAAg                                                  >  1:1588739/1‑140 (MQ=255)

                                                                                         atcaCAGTACTGCTTGTTTTAGTTGTAACGAATCTGGAGTCGATCATTTCTTTGTTTCAAGGTGATGATGCGAAAGAAAAAATCGC‑CGTTGTTGTTGAAACAAACGAATTATATCCGATTTTCTAGAAGCAGCTGAAAGc                                                 >  2:6378956/1‑140 (MQ=255)

                                                                                         atcaCAGTACTGCTTGTTTTAGTTGTAACGAATCTGGAGTCGATCATTTCTTTGTTTCAAGGTGATGATGCGAAAGAAAAAATCGC‑CGTTGTTGATGAAACAAACGAATTATATCCGATTTTCTCGAAGCAGCTGAAAGc                                                 <  1:7429032/140‑1 (MQ=255)

                                                                                         atcaCAGTACTGCTTGTTTTAGTTGTAACGAATCTGGAGTCGATCATTTCTTTGTTTCAAGGTGATGATGCGAAAGAAAAAATCGC‑CGTTGTTGATGAAACAAACGAATTATATCCGATTTTCTCGAAGCAGCTGAAAGc                                                 <  1:2723448/140‑1 (MQ=255)

                                                                                         atcaCAGTACTGCTTGTTTTAGTTGTAACGAATCTGGAGTCGATAATTTCTTTGTTTCAAGGTGAGGATGCGAAAGAAAAAATCGC‑CGTTGTTTATGAAACAAAAGACTTATATCCGCTTTTCTCGCAGCAGCGGAAAGc                                                 >  2:6265712/1‑140 (MQ=255)

                                                                                         atcaCAGTACTGATTGTTTTAGTTGTAACGAATCTGGAGTCGATCATTTCTTTTTTTCAAGGTGATGATGCGAAAGAAAAAATCGC‑CGTTGTTGATGAAACAAACGAATTATATCCGATTTTCTCGAAGCAGCTGAAAGc                                                 <  1:5891717/140‑1 (MQ=255)

                                                                                          tcaCAGTACTGCTTGTTTTAGTTGTAACGAATCTGGAGTCGATCATTTCTTTGTTTCAAGGTGATGATGCGAAAGAAAAAATCGC‑CGTTGTTGATGAAACAAACGAATTATATCCGATTTTCTCGAAGCAGCTGAAAGCg                                                <  2:3350865/140‑1 (MQ=255)

                                                                                          tcaCAGTACGGCTTGTTTTAGTTGTAACGAATCTGGAGTCGATCATTTCTTTGTTTCAAGGTGATGATGCGAAAGAAAAAATCGC‑CGTTGTTGATGAAACAAACGAATTATATCCGATTTTCTCGAAGCAGCTGAAAGCg                                                <  2:6514029/140‑1 (MQ=255)

                                                                                             ccgTACTGCTTGTTTTATTTGTAACGAATCTGGAGGCGATCATTTCTTTGTTACAAGGTGATGATGCGAAAGAAAAAATCGC‑CGTTGTTGATGAAACAAACGAATTATATCCGATTTTCTCGAAGCAGCTGAAAGCGGTg                                             <  2:4772320/138‑1 (MQ=255)

                                                                                             caGTACTGCTTGTTTTTGTTGTAACGAATCTGGAGTCGATAATTTCTTTGTTTCAAGGTGATGATGCGAAAGAAAAAATCGC‑CGTTGTTGATGAAACAAACGAATTATATCCGATTTTCTCGAAGCAGCTGAAAGCGGTg                                             <  1:1761477/140‑1 (MQ=255)

                                                                                             aattACTGCTTTTTTTAGTTGTAACTAATCTGGAGTCGATCATTTCTTTTTTTCACGGTGATGATGCTAAAGAAAAAATCGC‑CGTTGTTGATGAAACAAACGAATTATATCCGATTTTCTCGAAGCAGCTGAAAGCGGTg                                             <  1:2970537/137‑1 (MQ=255)

                                                                                                   tGCTTGTTTTAGTTGTAACGAATCTGGAGTCGATCATTTCTTTGTTTCAAGGTGATGATGCGAAAGAAAAAATCGC‑CGTTGTTGATGAAACAAACGAATTATATCCGATTTTCTCGAAGCAGCTGAAAGCGGTGGATACa                                       >  2:3540507/1‑140 (MQ=255)

                                                                                                   tGCTTGTTTTAGTTGTAACGAATCTGGAGTCGATCATTTCTTTGTTTCAAGGTGATGATGCGAAAGAAAAAATCGC‑CGTTGTTGATGAAACAAACGAATTATATCCGATTTTCTCGAAGCAGCTGAAAGCGGTGGATACa                                       >  2:5962431/1‑140 (MQ=255)

                                                                                                   tGCTTGTTTTAGTTGTAACGAATCTGGAGTCGATCATTTCTTTGTTTCAAGGTGATGATGCGAAAGAAAAAATCGC‑CGTTGTTGATGAAACAAACGAATTATATCCGATTTTCTCGAAGCAGCTGAAAGCGGTGGATACa                                       >  2:5238734/1‑140 (MQ=255)

                                                                                                   tGCTTGTTTTAGTTGTAACGAATCTGGAGTCGATCATTTATTTTTTTCAAGGTGATGATGCGAAAGAAAAAATAGC‑CGTTGTTTATTAAACAAAACAAATATATACGATTTTCTCGAAGCAGCTGAAAGCGGTGGATACa                                       >  1:1180309/1‑140 (MQ=255)

                                                                                                     cTTGTTTTAGTTGTAACGAATCTGGAGTCGATCATTTCTTTGTTTGAAGGTGATGATGCGAAAGAAAAAATCGC‑CGTTGTTGATGAAACAAACTAATTATATCCGATTTTATCGAAGCAGCTGAAATCGGTGGATACaaa                                     >  1:5957786/1‑140 (MQ=255)

                                                                                                     cTTGTTTTAGTTGTAACGAATCTGGAGTCGATCATTTCTTTGTTTCAAGGTGATGATGCGAAAGAAAAAATCGC‑CGTTGTTGATGaaa                                                                                         >  1:4636439/1‑88 (MQ=255)

                                                                                                     cTTGTTTTAGTTGTAACGAATCTGGAGTCGATCATTTCTTTGTTTCAAGGTGATGATGCGAAAGAAAAAATCGC‑CGTTGTTGATGaaa                                                                                         <  2:4637235/88‑1 (MQ=255)

                                                                                                     cTTGTTTTAGTTGTAACGAATCTGGAGTCGATCATTTCTTTGTTTCAAGGTGATGATGCGAAAGAAAAAATCGC‑CGTTGTTGATGAAACAAACGAATTATATCCGATTTTGTCGAAGCAGCTGTAAGCGGTGGATACaaa                                     >  1:7754951/1‑140 (MQ=255)

                                                                                                     cTTGTTTTAGTTGTAACGAATCTGGAGTCGATCATTTCTTTGTTTCAAGGTGATGATGCGAAAGAAAAAATCGC‑CGTTGTTGATGAAACAAACGAATTATATCCGATTTTCTCGAATCAGCTGAAAGCGGTGGATACaaa                                     >  2:4290394/1‑140 (MQ=255)

                                                                                                     cTTGTTTTAGTTGTAACGAATCTGGAGTCGATCATTTCTTTGTTTCAAGGTGATGATGCGAAAGAAAAAATCGC‑CGTTGTTGATGAAACAAACGAATTATATCCGATTTTCTCGAAGCAGCTGAAAGCGGTGGATACaaa                                     >  2:7512774/1‑140 (MQ=255)

                                                                                                     cTTGTTTTAGTTGTAACGAATCTGGAGTCGATCATTTCTTTGTTTCAAGGTGATGATGCGAAAGAAAAAATCGC‑CGTTGTTGATGAAACAAACGAATTATATCCGATTTTCTCGAAGCAGCTGAAAGCGGTGGATACaaa                                     <  2:1703878/140‑1 (MQ=255)

                                                                                                     cTTGTTTTAGTTGTAACGAATCTGGAGTCGATCATTTCTTTGTTTCAAGGTGATGATGCGAAAGAAAAAATCGC‑CGTTGTTGATGAAACAAAAGAATTATATCCGATTTTCTCGAAGCAGCTGAAAGCGGTGGATACaaa                                     >  2:5723604/1‑140 (MQ=255)

                                                                                                     cTTGTTTTAGTTGTAACGAATCTGGAGTCGATCATTTCTTTGTTTCAAGGTGATGATGCGAAAGAAAAAATCGC‑CCTTGTTGATGAAACAAACGAATTATATCCGATTTTATCGAAGCAGCGGAAAGCGGTGGATACaca                                     >  1:4424635/1‑138 (MQ=255)

                                                                                                     cTTGTTTTAGTTGTAACGAATCTGGAGTCGATCATTTCTTTGTTTAAAGGTGATGATGCGAAAGAAAAAATCGC‑CGTTGTTTATGAAACAAACGAATTATATCCGATTTTCTCGAAGCAGCTGAAAGCGGTTGATACaaa                                     >  2:2079128/1‑140 (MQ=255)

                                                                                                     cTTGTTTTAGTTGTAACGAATCTGGAGTCGATCATTTATTTTTTTCAAGGTGATGATTCGAAATAAAAAATAGC‑CGTTGTTTATGAAAAAAACGAATTATATACGATTTTCTAGTAGCAACTTGAAGCGGTTGATACaaa                                     >  1:6690703/1‑140 (MQ=255)

                                                                                                     cTTGTTTTAGTTGTAACGAATCTGGAGTCGATCATTTATTTTTTTCAAGGTGATGATTCGAAAGAAAAAATCGC‑CGTTGTTGATGAAACAAACGAATTATATACGATTTTATCGAAGCAGCTGAAAGCGGTGGATACcaa                                     >  1:507466/1‑140 (MQ=255)

                                                                                                     cTTGTTTTAGTTGTAACGAATCTGGAGTCGATCATTTATTTGTTTGAAGGTGATGATTCGAAAGAAAAAAAAGC‑CGTTGTTGTTTAAACAAACGAAATATAAACGCTTTTCAAGAAACACATGAAAGCGGTGGATACaaa                                     >  1:1007950/1‑140 (MQ=255)

                                                                                                     cTTGTTTTAGTTGTAACGAATCTGGAGTCGATCATTTATTTGTTTGAAGGTGATGATGCGAAAGAAAAAATAGC‑CGTTGTTGATGAAACAAACGGAGTATATCCCATTTTCTCGAAGCAGCTGAAAGCGGTGGATACaaa                                     >  1:3089725/1‑140 (MQ=255)

                                                                                                     cTTGTTTTAGTTGTAACGAATCTGGAGTCGATCATTTATTTGTTTCAAGGTGGTGATGCGCAAGAAAAAATAGC‑CCTTGTTGGTTAAAAAAAAGAATTATATACGATTTTCTCGAAGCAGCTGAAAGCGGTGGATACaaa                                     >  1:2585410/1‑140 (MQ=255)

                                                                                                     cTTGTTTTAGTTGTAACGAATCTGGAGTCGATCATTTATTTGTTTAAATGTGATGATGCGAAAGTAAAAATCGC‑CGTTGTTGATGAAACAAACGACTTATATCCGAATTTATAGAAGCAGCTGAAACCGGTGGATACaaa                                     >  1:4255136/1‑140 (MQ=255)

                                                                                                        gTTTTAGTTGTAACGAATCTGGAGTCGATCATTTCTTTGTTTCAAGGTGATGATGCGAAAGAAAAAATCGC‑CGTTGTTGATGAAACAAACGAATTATATCCGATTTTCTCGAAGCAGCTGAAAGCGGTGGATACAAACgg                                  <  2:6815793/140‑1 (MQ=255)

                                                                                                        gTTTTAGTTGTAACGAATCTGGAGTAGATCATTTCTTTGTTTCAAGGTGATGATGCGAAAGAAAAAATCGC‑CGTTGTTGATGAAACAAACGATTTATATCCGATTTTCTCGAAGCAGCTGAAAGCGGTGGATACAAACgg                                  <  2:1434254/140‑1 (MQ=255)

                                                                                                          tttAGTTGTAACGAATCTGGAGTCGATCATTTATTTGGTTCAAGGTGATGATGCGAAAGCAAAAATCGC‑CCGTGTTGATTAAACAAACGACATATATACGATTTTCTAGTAGCAGCTGAAAGCGGGGGATACAAACgggg                                >  1:4124694/1‑138 (MQ=255)

                                                                                                             aGTTGTAACGAATCTGGAGTCGATCATTTCTTTTTTTCAAGGTGATGATGCGAAAGAAAAAATCGC‑CGTTGTTGATGAAACAAACGAATTATATCCGATTTTCTCGAAGCAGCTGAAAGCGGTGGATACAAACGGTGATc                             >  1:5463390/1‑140 (MQ=255)

                                                                                                             aGTTGTAACGAATCTGGAGTCGATCATTTCTTTGTTTGAAGGTGATGATGCGAAAGAAAAAATCGC‑CCTTGTTGATGTAAAAAAAGAATTATATCCGATTTTATAGAAGCAGCGGAAATCGGTGGATAAAAACGGTGATc                             >  2:3198082/1‑140 (MQ=255)

                                                                                                             aGTTGTAACGAATCTGGAGTCGATCATTTCTTTGTTTGAAGGTGATGATGCGAAAGAAAAAATCCC‑CGTTGTTGATGAAACAAACGCATTATCTCCGATTTTCTCGAAGCACCTGAAAGCGGTGGAGACAAACGGTGATc                             >  2:4927601/1‑140 (MQ=255)

                                                                                                              gTTGTAACGAATCTGGAGTCGATCATTTCTTTGTTTCAAGGTGATGATGCGAAAGAAAAAATCGC‑CGTTGTTGATGAAACAAACGAATTATATCCGATTTTCTCGAAGCAGCTGAAAGCGGTGGATACAAACGGTGATCt                            <  1:3741521/140‑1 (MQ=255)

                                                                                                              gTTGTAACGAATCTGCAGGCGATCATTTCTTTGTTTCAAGGTGATGATGCGAAATAAAAAATCGC‑CGTTGTTGATGAAACAAACGAATTATATCCGATTTTCTCGAAGCAGCTGAAAGCGGTGGATACAAACGGTGATCt                            <  2:6587144/140‑1 (MQ=255)

                                                                                                               ttGTAACGAATCTGGAGTCGATCATTTCTTTTTTTAAAGGTGATGATGCGAAAGTAAAAAAAGCACGTTTTTTAT‑AAACAAACGAATTATATCCGAATTTCTAGAATCACCTGAAAGCGGTGGATACAAACGGTGATCtt                           >  1:679016/1‑140 (MQ=255)

                                                                                                                 gTAACGAATCTGGAGTCGATCATTTATTTGTTTCAAGGTGATGATGCGAAAGAAAAAATCGC‑CGTTTTTGATGAAACAAAAGAATTATAACCGATTTTCTCGAAGCAGCTGAAAGCGGTGGATACAAACGGTGATCTTGa                         >  2:28265/1‑140 (MQ=255)

                                                                                                                    aCGAATCTGGAGTCGATCATTTATTTGTTTGAAGGTGATTATGCGAAATAAAAAATAGC‑CGGTGTTGATTAAAAAAACAAAATATATCCGATTTTCTCGAAGCACCTGAAAGCGGTGGATACAAACCGTGATATTGAtgt                      >  1:6231098/1‑140 (MQ=255)

                                                                                                                       aaTCTGTTGTCGATCATTTCTTTGTTTAAAGGTGATGATGCGAAATAAAAAATCGC‑CGTTGTTGATGAAACAAACGAATTATATCCGATTTTCTCGAAGCAGCTGAAAGCGGTGGATACAAACGGTGATCTTGATGTGaa                   <  2:2266687/140‑1 (MQ=255)

                                                                                                                       aaTCTGGAGTCGATCATTTCTTTGTTTCAAGGTGATGATGCGAAAGAAAAAATCGC‑CGTTGTTGATGAAACAAACGAATTATATCCGATTTTCTCGAAGCAGCTGAAAGCGGTGGATACAAACGGTGATCTTGATGTGaa                   <  2:6125586/140‑1 (MQ=255)

                                                                                                                       aaTCTGGAGTCGATCATTTCTTTGTTTCAAGGTGATGATGCGAAAGAAAAAATCGC‑CGTTGTTGATGAAACAAACGAATTATATCCGATTCTCTCGAAGCAGCTGAAAGCGGTGGATACAAACGGTGATCTTGATGTGaa                   <  2:7367522/140‑1 (MQ=255)

                                                                                                                         tCTGGAGTCGATCATTTCTTTGTTTCAAGGTGATGATGCGAAAGAAAAAATCGC‑CGTTGTTGATGAAACAAACGAATTATATCCGATTTTCTCGAAGCAGCTGAAAGCGGTGGATACACACGGTGATCTTGATGTGAAGc                 >  2:2352241/1‑140 (MQ=255)

                                                                                                                            ggAGTCGATCATTTCTTTGTTTCAAGGTGATGATGCGAAAGAAAAAATCGC‑CGTTGTTGATGAAACAAACGAATTATATCCGATTTTCTCGAAGCAGCTGAAAGCGGTGGATACAAACGGTGATCTTGATGTGa                    >  2:1317906/1‑134 (MQ=255)

                                                                                                                             gAGTCGATCATTTCTTTGTTTCAAGGTGATGATGCGAAAGAAAAAATCGC‑CGTTGTTGATGAAACAAACGAATTATATCCGATTTTCTCGAAGCAGCTGAAAGCGGTGGATACAAACGGTGATCTTGATGTGAAGCAATc             <  1:7645179/140‑1 (MQ=255)

                                                                                                                                 cGATCATTTCTTTGTTTCAAGGTGATGATGCGAAAGAAAAAATCGC‑CGTTGTTGATGAAACAAACGAATTATATCCGATGTTCTCGAAGCAGCTGAAAGCGGTGGATACAAACGGTGATCTTGATGTGAAGCAATCAAAg         <  2:4544571/140‑1 (MQ=255)

                                                                                                                                   aTCATTTCTTTGTTTCCACGTGATGATGCGAAAGAAAAAATCGC‑CGTTGTTGATGAAACAAACGAATTATATCCGATTTTCTCGAAGCAGCTGAAAGCGGTGGATACAAACGGTGATCTTGATGTGAAGCAATCAAAGCt       >  2:703020/1‑140 (MQ=255)

                                                                                                                                   aTCATTTCTTTGTTTCAAGGTGATGATGCGAAAGAAAAAATCGC‑CGTTGTTGATGAAACAAACGAATTATATCCGATTTTCTCGAAGCAGCTGAAAGCGGTGGATACAAACGGTGATCTTGATGTGAAGCAATCAAAGCt       <  1:2336687/140‑1 (MQ=255)

                                                                                                                                        ttCTTTGTTTCAAGGTGATGATGCGAAAGAAAAAATCGC‑CGTTGTTGATGAAACAAACGAATTATATCCGATTTTCTCGAAGCAGCTGAAAGCGGTGGATACAAACGGTGATCTTGATGTGAAGCAATCAAAGCTATCgg  <  2:5539276/140‑1 (MQ=255)

GGGGGCCGTCTATGAATAAATTTTGGATCATGCTCTCTCATACATACAAAAATAAGATTATGGCTAAATCTTTTATTATTTCGACTGTCATCACAGTACTGCTTGTTTTAGTTGTAACGAATCTGGAGTCGATCATTTCTTTGTTTCAAGGTGATGATGCGAAAGAAAAAATCGC‑CGTTGTTGATGAAACAAACGAATTATATCCGATTTTCTCGAAGCAGCTGAAAGCGGTGGATACAAACGGTGATCTTGATGTGAAGCAATCAAAGCTATCGG  >  NZ_CP034943/1036145‑1036420

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`