BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	1,720,748	C→A	8.9%	S224R (AGC→AGA)	spoIIIE →	DNA translocase SpoIIIE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	1,720,748	0	C	A	8.9%	686.3 / 7.8	282	S224R (AGC→AGA)	spoIIIE	DNA translocase SpoIIIE
Reads supporting (aligned to +/- strand): ref base C (137/107); new base A (5/33); total (142/140)
Fisher's exact test for biased strand distribution p-value = 4.17e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 297 total aligned reads displayed.

GCGCTCTCTTCAAGAAACGCTGAAAAAGTGGATGAGCCCGATTGGCCGCTTTATAAAAGAACAATGG‑TTT‑GCATTTATTGATGATATGAAATCCGTCAAAACCAATATGAAGTCATCGAAAAAAACGAAAGCGCCGAGCAAAAAACAAAAAACGGCCCGCAAAAAACAGCAAATGGAACCGGAGCCTTCTGCTGAAGAGGGGGATTTGGAAACGGTATCGCCTCTTATTCATTCAGAGCCGATTATCTCAAGCTTTTCAGATCGTAATGAAGATGAAG  >  NZ_CP034943/1720610‑1720887

   ctctctTCAAGAAACGCTGAAAAAGTGGATGAGCCCGATTGGCCGCTTTATAAAAGAACAATGG‑TTT‑GCATTTATTGATGATATGAAATCCGTCAAAACCAATATGAAGTCATCGAAAAAAACGAAAGCGCCGAGcaaaa                                                                                                                                         >  1:6268255/1‑140 (MQ=255)

       ctTCAAGAAACGCTGAAAAAGTGGATGAGCCCGATTGGCCGCTTTATAAAAGAACAATGG‑TTT‑GCATTTATTGATGATATGAAATCCGTCAAAACCAATATGAAGTCATCGAAAAAAACGAAAGCGCCGAGcaaaaaaca                                                                                                                                     >  1:1216363/1‑140 (MQ=255)

             gAAACGCTGAAAAAGTGGATGAGCCCGATTGGCCGCTTTATAAAAGAACAATGG‑TTT‑GCATTTATTGATGATATGAAATCCGTCAAAACCAATATGAAGTCATCGAAAAAAACGAAAGCGCCGAGAaaaaaacaaaaaac                                                                                                                               >  2:4744068/1‑140 (MQ=255)

             gAAACGCTGAAAAAGTGGATGAGCCACATTGGCCGCTTTATAAAAGAACAATGG‑TTT‑GCATTTATTGATGATATGAAATCCGTCAAAACCAATATGTAGTCATCCAAAAAAACGAAAGCTCGGAGCaaaaatcaaaaaac                                                                                                                               >  2:2982638/1‑140 (MQ=255)

                aCGCTGAAAAAGTGGATGAGCCCGATTGGCCGCTTTATAAAAGAACAATGG‑TTT‑GCATTTATTGATGATATGAAATCCGTCAAAACCAATATGAAGTCATCGAAAAAAACGAAAGCGCCGAGCAAAAAACAAAAAACGGc                                                                                                                            >  1:786203/1‑140 (MQ=255)

                aCGCTGAAAAAGTGGATGAGCCCGATTGGCCGCTTTATAAAAGAACAATGG‑TTT‑GCATTTATTGATGATATGAAATCCGGCAAAACCAATATGAAGTCATCGAAAAAAACGAAAGCGCCGAGCAAAAAACAAAAAACGGc                                                                                                                            >  2:5035681/1‑140 (MQ=255)

                  gCTGAAAAAGTGGATGAGCCCGATTGGCCGCTTTATAAAAGAACAATGG‑TTT‑GCATTTATTGATGATATGAAATCCGTCAAAACCAATATGAAGTCATCGAAAAAAACGAAAGCGCCGAGCAAAAAACAAAAAACGGccc                                                                                                                          >  1:5754766/1‑140 (MQ=255)

                  gCTGAAAAAGTGGATGAGCCCGATTGGCCGCTTTATAAAAGAACAATGG‑TTT‑GCATTTATTGATGATATGAAATCCGTCAAAACCAATATGAAGTCATCGAAAAAAACGAAAGCGCCGAGCAAAAAACAAAAAACGGccc                                                                                                                          >  2:1472070/1‑140 (MQ=255)

                  gCTGAAAAAGTGGATGAGCCCGATTGGCCGCTTTATAAAAGAACAATGG‑TTT‑GCATTTATTGATGATATGAAATCCGTCAAAACCAATATGAAGTCATCGAAAAAAACGAAAGCGCCGAGCAAAAAACAAAAAACGGccc                                                                                                                          >  2:4654776/1‑140 (MQ=255)

                  gCTGAAAAAGTGGATGAGCCCGATTGGCCGCTTTATAAAAGAACAATGG‑TTT‑GCATTTATTGATGATATGAAATCCGTCAAAACCAATATGAAGTCATCGAAAAAAACGAAAGCGCCGAGCAAAAAACAAAAAACGGccc                                                                                                                          >  2:5353088/1‑140 (MQ=255)

                  gCTGAAAAAGTGGATGAGCCCGATTGGCCGCTTTATAAAAGAACAATGG‑TTT‑GCATTTATTGATGATATGAAATCCGTCAAAACCAATATGAAGTCATCGAAAAAAACGAAAGCGCCGAGCAAAAAACAAAAAACGGccc                                                                                                                          >  2:6772089/1‑140 (MQ=255)

                  gCTGAAAAAGTGGATGAGCCCGATTGGCCGCTTTATAAAAGAACAATGG‑TTT‑GCATTTATTGATGATATGAAATCCGTCAAAACCAATATGAAGTCATCGAAAAAAACGAAAGCGCCGAGCAAAAAACAAAAAACGGccc                                                                                                                          >  1:3077097/1‑140 (MQ=255)

                  gCTGAAAAAGTGGATGAGCCCGATTGGCCGCTTTATAAAAGAACAATGG‑TTT‑GCATTTATTGATGATATGAAATCCGTCAAAACCAATATGAAGTCATCGAAAAAAACGAAAGCGCCGAGCAAAAAACAAAAAACGGccc                                                                                                                          >  2:6927151/1‑140 (MQ=255)

                  gCTGAAAAAGTGGATGAGCCCGATTGGCCGCTTTATAAAAGAACAATGG‑TTT‑GCATTTATTGATGATATGAAATCCGTCAAAACCAATATGAAGTCATCGAAAAAAACGAAAGCGCCGAGCAAAAAACAAAAAACGGccc                                                                                                                          >  2:699374/1‑140 (MQ=255)

                  gCTGAAAAAGTGGATGAGCCCGATTGGCCGCTTTATAAAAGAACAATCG‑TTT‑GCATTTATTGATGATATGAAATCCGTCAAAACCAAAATGAAGTCATCGAAAAAAACGAAAGCGCCGAGCAAAAAACAAAAAACGGccc                                                                                                                          >  1:2274396/1‑140 (MQ=255)

                            tGGATGAGCCCGATTGGCCGCTTTATAAAAGAACAATGG‑TTT‑GCATTTATTGATGATATGAAATCCGTCAAAACCAATATGAAGTCATCGAAAAAAACGAAAGCGCCGAGCAAAAAACAAAAAACGGCCCGCAAAAAACa                                                                                                                >  1:4550960/1‑140 (MQ=255)

                              gATGAGCCCGATTGGCCGCTTTATAAAAGAACAATGG‑TTT‑GCATTTATTGATGATATGAAATCCGTCAAAACCAATATGAAGTCATCGAAAAAAACGAAAGCGCCGAGCAAAAAACAAAAAACTGCCCGCAAAAAACAGc                                                                                                              >  2:1955955/1‑140 (MQ=255)

                              gATGAGCCCGATTGGCCGCTTTATAAAAGAACAATGG‑TTT‑GCATTTATTGATGATATGAAATCCGTCAAAACCAATATGAAGTCATCGAAAAAAACGAAAGCGCCGAGCAAAAAACAAAAAACGGCCCGCAAAAAACAGc                                                                                                              >  1:6104427/1‑140 (MQ=255)

                                 tcgCCCGTTTGGCCGCTTTATACAATAAAATTGG‑TTA‑ACATTTATGTATTATAGTAAATCCGTAAAACCAAATATGAAGTCATCGAAAAAAACCAAACCGCCGAGCAAAAAACAAAAAACGGCCCGCAAAAAACAGCaaa                                                                                                           <  1:5572627/138‑1 (MQ=255)

                                 gcgCCCGTTTGGCCGGTTTATAAAATAA‑AATGGTTTT‑TCTTTTATTGTTCATTTTAAATCCTTAAAAACAATTATGATGACATCTAAAAAAACGAACGCCCCGAGCAAAAAAAAAAAAACGGCCCGCAAAAAACAGCaaa                                                                                                           <  1:7058802/138‑1 (MQ=255)

                                  agccgtaTTGGCCGTTTTATAAAATAACAATGG‑TTT‑GAATTTATTTATGATATGAAATCCGACAAAACAAATATGAAGTCATCGAAAAAAACGAACGCGCCGAGCAAAAAACAAAAAACGGCCCGCAAAAAACAGCAAAt                                                                                                          <  2:6836617/134‑1 (MQ=255)

                                  aGCCCGATTGGCCGCTTTATAAAAGAACAATGG‑TTT‑GCATTTATTGATGATATGAAATCCGTCAAAACAAATATGAAGTCATCGAAAAAAACGAAAGCGCCGAGCAAAAAACAAAAAACGGCCCGCAAAAAACAGCAAAt                                                                                                          <  2:3840185/140‑1 (MQ=255)

                                      cGATTGGCCGCTTTATAAAAGAACAATGG‑TTT‑GCATTTATTGATGATATGAAATCCGTCAAAACCAATATGAAGTCATCGAAAAAAACGAAAGCGCCGAGCAAAAAACAAAAAACGGCCCGCAAAAAACAGCAAATGGaa                                                                                                      >  1:4814379/1‑140 (MQ=255)

                                      cGATTGGCCGCATTATAAAATAACAATGG‑TTT‑GCATTTATTGATCATATCAAAGCCGTAAAAACCAATATCAAGACATCGAAAAAAACGAAAGCGCCGAGCAAAAAACAAAAAACGGCCCGCAAAAAACAGCAAATGGaa                                                                                                      <  2:6598794/140‑1 (MQ=255)

                                        aTTGGCCGCTTTATAAAAGAACAATGG‑TTT‑GCATTTATTGATGATATGAAATCCGTCAAAACCAATATGAAGTCATCGAAAAAAACGAAAGCGCCGAGCAAAAAACAAAAAACGGCCCGCAAAAAACAGCAAATGGAAcc                                                                                                    >  1:1833991/1‑140 (MQ=255)

                                           ggCCGATTTAAAAAACAAAACTGG‑TTA‑CCATTTATTGATTATATCAAACCCGACAAAACAAATATCAACCCATCAAAAAAACCCCACGCCCCCCCCAAACAACAAAAAACGGCCCGCAAAAAACAGCAAATGGAACCGGa                                                                                                 <  1:2210169/140‑1 (MQ=255)

                                           gccctcTTTATAAAACAAACATGG‑TTT‑GAATTTATTGAGTATATTAAAGCCGACAAAACCAATATGAACTCATCTAAAAAAACCAAAGCGCCGAGCAAAAAACAAAACACGGCCCGCAAAAAACAGCAAATGGAACCGGa                                                                                                 <  1:4479905/135‑1 (MQ=255)

                                              ctatttATAAAAGAACATTGG‑TTT‑GCATTTATTGATGCTTTTAAATCCGAAAAAACAAATATGAAGACAGCTAAAAAAACGAACGCGCCGCGCAAAAAACAAAAAACGGCCCGCAAAAAACAGCAAATGGAACCGGAGcc                                                                                              <  1:5351131/137‑1 (MQ=255)

                                              cgttttATAAAACAAC‑ATGG‑TTTGGCTTTTATTTATCATATCAAACCCGACAAAACAAATATCAAGTAATCCAAAAAAACGAACGCGCCGAGAAAAAAACAAAACACGGCCCGCAAAAAACAGCAAATGGAACCGGAGcc                                                                                              <  1:218487/137‑1 (MQ=255)

                                              cgatttAAAAAATAACAATGG‑TTT‑GAATTTATTGATGATATGAAATCCGTCAAAACCAATATGAAGTCATCGAAAAAAACGAACGCGCCGAGCAAAAAACAAAACACGGCCCGCAAAAAACAGCAAATGGAACCGGAGcc                                                                                              <  2:7275850/137‑1 (MQ=255)

                                              cgatttAAAAAACAAAAATGT‑TTT‑GCATTTATTGATGATATCAAATCCGTCAAAACAAATATGAATTAATCTAAAAAAACGAAAGCGCCGAGAAAAAAACAAAAAACGGCCCGCAAAAAACAGCAAATGGAACCGGAGcc                                                                                              <  1:5679968/137‑1 (MQ=255)

                                              cGCTTTATAAAAGAACAATGG‑TTT‑GCATTTATTGATGATATGAAATCCGTCAAAACCAATATGAAGTCATCGAAAAAAACGAAAGCGCCGAGCAAAAAACAAAAAACGGCCCGCAAAAAACAGCAAATGGAACCGGAGcc                                                                                              >  2:2552099/1‑140 (MQ=255)

                                              cGCTTTATAAAAGAACAATGG‑TTT‑GCATTTATTGATGATATGAAATCCGTCAAAACCAATATGAAGTCATCGAAAAAAACGAAAGCGCCGAGCAAAAAACAAAAAACGGCCCGCAAAAAACAGCAAATGGAACCGGAGcc                                                                                              >  1:5523064/1‑140 (MQ=255)

                                              cGCTTTATAAAAGAACAATGG‑TTT‑GAATTTATTGATGATATGAAATCCGTCAAAACCAATATGAAGTCATCGAAAAAAACGAAAGCGCCGAGCAAAAAACAAAAAACGGCCCGCAAAAAACAGCAAATGGAACCGGAGcc                                                                                              >  1:274590/1‑140 (MQ=255)

                                              cGCTTTATAAAAGAACAATGG‑TTT‑GAATTTATTGATGATATCAAATCCGAAAAAACAAATATGAAGTCATCTAAAAAAACGAACGCGCCGAGCAAAAAACAAAAAACGGCCCGCAAAAAACAGCAAATGGAACCGGAGcc                                                                                              <  2:6214643/140‑1 (MQ=255)

                                                ttttAAAAAATAACATTGT‑TTT‑GAATTTATTGATTATTTCAAACCCTTAAAAACAAATATTAATTAATCTAAAAAAACCAAAGCGCCGAGAAAAAAACAAAAAACGGCCCGCAAAAAACAGCAAATGGAACCGGAGCCtt                                                                                            <  1:4012198/139‑1 (MQ=255)

                                                cTTTATAAAATAACATTGT‑TTT‑GAATTTATTGATGATAGCAAATCCGTCAAAACAAATATTAAGTCAGCTAAAAAAACGAACGCGCCGAGAAAAAAACAAAAAACGGCCCGCAAAAAACAGCAAATGGAACCGGAGCCtt                                                                                            <  1:3710269/140‑1 (MQ=255)

                                                cTTTATAAAACAACATTGG‑TTT‑GAATTTATTTATGATATCAAATCCGAAAAAACAAATATGAAGTCATCGAAAAAAACCACCGCGCCGACAAAAAAACAAAAAACGGCCCGCAAAAAACAGCAAATGGAACCGGAGCCtt                                                                                            <  1:5240866/140‑1 (MQ=255)

                                                 tttATAAAAGAACAATGG‑TTT‑GCATTTATTGATGATATGAAATCCGTCAAAACCAATATGAAGTCATCGAAAAAAACGAAAGCGCCGAGCAAAAAACAAAAAACGGCCCGCAAAAAACAGCAAATGGAACCGGAGCCTTc                                                                                           >  2:4472556/1‑140 (MQ=255)

                                                 tttATAAAAGAAAAATGG‑TTT‑GCATTTATTGATGATATGAAATCCGTCAAAACCAAAATGAAGTCATCGAAAAAAACGAAAGCGCCGAGCAAAAAACAAAAAACGGCCCGCAAAAAACAGCAAATGGAACCGGAGCCTTc                                                                                           >  2:3749485/1‑140 (MQ=255)

                                                   aacaaaaaaACCTTGG‑TTT‑GAATTTTTTGATGATATCAAATCCGACAAAACAAATATCAAGTCAGCTAAAAAACCCAACGCCCCGCCAAAAAAACAAAAAACGGCCCGCAAAAAACAGCAAATGGAACCGGAGCCTTctg                                                                                         <  1:6478881/137‑1 (MQ=255)

                                                     aaaaaCAACATTGT‑TTT‑TAATTTTTTGTTTATATCAAATCCGAAAAAACAAATTTGATGTAATCCAAAAAAACCAACCCGCCGCCCAAAAAACAAAAAACGGCCCGCAAAAAACAGCAAATGGAACCGGAGCCTTctgct                                                                                       <  1:725224/139‑1 (MQ=255)

                                                           aaCAATGT‑TTT‑GCATTTATTGATTATATGAAATCCTTCAAAACAAATATGAAGTCATAGAAAAAAACGAAAGCGCCGAGCAAAAAACAAAAAACGGCCCGCAAAAAACAGCAAATGGAACCGGAGCCTTCTGCTGAagag                                                                                 <  2:5555217/140‑1 (MQ=255)

                                                           aaCAATGG‑TTT‑GCATTTATTGATGATATGAAATCCGTCAAAACCAATATGAAGTCATCGAAAAAAACGAAAGCGCCGAGCAAAAAACAAAAAAACGCCCGCAAAAAACAGCAAATGGAACCGGAGCCTTCTGCAGTagag                                                                                 >  2:2138110/1‑140 (MQ=255)

                                                           aaCAATGG‑TTT‑GCATTTATTGAGGATATGAAATCCGTCAAAACCAATATGAAGTCATCGAAAAAAACGAAAGCGCCGAGCAAAAAACAAAAAACGGCCCGCAAAAAACACCAAATGGAACCGGAGCCTTCTGCTGAagag                                                                                 >  2:3354378/1‑140 (MQ=255)

                                                             cAATGG‑TTT‑GCATTTATTGATGATATGAAATCCGTCAAAACCAATATGAAGTCATCGAAAAAAACGAAAGCGCCGAGCAAAAAACAAAAAACGGCCCGCAAAAAACAGCAAATGGAACCGGAGCCTTCTGCTGAAGAggg                                                                               >  1:5289990/1‑140 (MQ=255)

                                                             cAATGG‑TTT‑GCATTTATTGATGATATGAAATCCGTCAAAACCAATATGAAGTCATCGAAAAAAACGAAAGCGCCGAGCAAAAAACAAAAAACGGCCCGCAAAAAACAGCAAATGGAACCGGAGCCTTCTGCTGAAGAggg                                                                               >  1:1412629/1‑140 (MQ=255)

                                                                ttg‑TTT‑GCATTTATTGATGATATGAAATCCGTCAAAACCAATATGACGTCATCGAAAAAAACGAAAGCGCCGAGCAAAAAACAAAAAACGGCCCGCAAAAAACAGCAAATGGAACCGGAGCCTTCTGCTGAAGAGGGGGa                                                                            <  2:1893780/138‑1 (MQ=255)

                                                                tGG‑TTT‑GCATTTATTGATGATATGAAATCCGTCAAAACCAATATGAAGTCATCGAAAAAAACGAAAGCGCCGAGCAAAAAACAAAAAACGGCCCGCAAAAAACAGCAAATGGAACCGGAGCCTTCTGCTGAAGAGGGGGa                                                                            <  2:5691473/140‑1 (MQ=255)

                                                                 tgtttt‑GCATTTATTGATGATATCAAATCCGTAAAAACAAATATGAAGTCATCGAAAAAAACGAACCCGCCGAGCAAAAAACAAAAAACGGCCCGCAAAAAACAGCAAATGGAACCGGAGCCTTCTGCTGAAGAGGGGGa                                                                            <  2:2777435/137‑1 (MQ=255)

                                                                      t‑GCATTTATTGATGATATGAAATCCGTCAAAACCAATATGAAGTCATCGAAAAAAACGAAAGCGCCGAGCAAAAAACAAAAAACGGCCCGCCAAAAACAGCAAATGGCACCGGAGCCTTCTGCTGAAGAGGGGGAGTTgg                                                                       >  1:4275327/1‑140 (MQ=255)

                                                                      t‑GCATTTATTGATGATATGAAATCCGTCAAAACCAATATGAAGTCATCGAAAAAAACGAAAGCGCCGAGCAAAAAACAAAAAACGGCCCGCAAAAAACAGCAAATGGAACCGGAGCCTTCTGCTGAAGAGGGGGATTTgg                                                                       >  1:6734897/1‑140 (MQ=255)

                                                                      t‑GCATTTATTGATGATATGAAATCCGTCAAAACCAATATGAAGTCATCGAAAAAAACGAAAGCGCCGAGCAAAAAACAAAAAACGGCCCGCAAAAAACAGCAAATGGAACCGGAGCCTTCTGCTGAAGAGGGGGATTTgg                                                                       >  2:1231730/1‑140 (MQ=255)

                                                                      t‑GCATTTATTGATGATATGAAATCCGTCAAAACCAATATGAAGTCATCGAAAAAAACGAAAGCGCCGAGCAAAAAACAAAAAACGGCCCGCAAAAAACAGCAAATGGAACCGGAGCCTTCTGCTGAAGAGGGGGATTTgg                                                                       >  1:961702/1‑140 (MQ=255)

                                                                      t‑GCATTTATTGATGATATGAAATCCGTCAAAACCAATATGAAGTCATCGAAAAAAACGAAAGCGCCGAGCAAAAAACAAAAAACGGCCCGCAAAAAACAGCAAATGGAACCGGAGCCTTCTGCTGAAGAGGGGGATTTgg                                                                       >  1:688603/1‑140 (MQ=255)

                                                                        taaTTTATTGTTTATATCAAATCCGAAAAAACCAATATGAAGACATCTAAAAAAACCAAAGCGCCGAGAAAAAAACAAAACACGGCCCGCAAAAAACAGCAAATGGAACCGGAGCCTTCTGCTGAAGAGGGGGATTTGGa                                                                      <  1:6184686/138‑1 (MQ=255)

                                                                         cATTTATTGTTTATATGAAATCCTTCAAAACCAATATGAATTCATCTAAAAAAACGAAAGCTCCGAGAAAAAAACAAAAAACGGCCCGCAAAAAACAGCAAATGGAACCGGAGCCTTCTGCTGAAGAGGGGGATTTGGaa                                                                     <  2:3044172/140‑1 (MQ=255)

                                                                          aTTTATTGATGATATGAAATCCGTCAAAACCAATATGAAGTCATCGAAAAAAACGAAAGCGCCGAGCAAAAAACAAAAAACGGCCCGCAAAAAACAGCAAATGGAACCGGAGCCTTCTGCTGAAGAGGGGGATTTGGaaa                                                                    <  2:3652263/140‑1 (MQ=255)

                                                                          aTTTATTGATGATAGGAACTCCGTCAAACCCAATTTGAAGTCATCGAAAAAAACGAAAGCGCCGAGCAAAAAACAAAAAACGGCCCGCAAAAAACAGCAAATGGAACCGGAGCCTTCTGCTGAAGAGGGGGATTTGGaaa                                                                    <  2:2616148/140‑1 (MQ=255)

                                                                              aTTGATGATATGAAATCCGTCAAAACCCATATGAAGTCATCGAAAAAAACGAAAGCGCCGAGCAAAAAACAAAAAACGGCCCGCACAAAACAGCAAATGGAACCGGAGCCTTCTGCTGAAGAGGGGGATTTGGAAACGGt                                                                >  1:2034128/1‑140 (MQ=255)

                                                                              aTTGATGATATGAAATCCGTCAAAACCAATATGAAGTCATCGAAAAAAACGAAAGCGCCGAGCAAAAAACAAAAAACGGCCCGCAAAAAACAGCAAATGGAACCGGAGCCTTCTGCTGAAGAGGGGGATTTGGAAACGGt                                                                >  1:1301983/1‑140 (MQ=255)

                                                                              aTTGATCATATGAAATCCGTCAAAACAAATATGAAGTCATCGAAAAAAACGAAAGCGCCGAGCAAAAAACAAAAAACGGCCCGCAAAAAACAGCAAATGGAACCGGAGCCTTCTGCTGAAGAGGGGGATTTGGAAACGGt                                                                <  2:3419328/140‑1 (MQ=255)

                                                                               ttGATGATATGAAATCCCTCAAAACCAATATGAAGTCATCGAAAAAAACGAAAGCGCCGAGCAAAAAACAAAAAACGGCCCGCAAAAAACAGCAAATGGAACCGGAGCCTTCTGCTGAAGAGGGGGATTTGGAAACGGTa                                                               <  2:4589475/140‑1 (MQ=255)

                                                                                 tattatatGAAATCCGTAAAACCCAATATGAAGTCATCTAAAAAAACGAACGCGCCGAGAAAAAAACAAAAAACGGCCCGCAAAAAACAGCAAATGGAACCGGAGCCTTCTGCTGAAGAGGGGGATTTGGAAACGGTATc                                                             <  2:1429629/136‑1 (MQ=255)

                                                                                 gatgatATGAAATCCGTCAAAACCAATATGAAGTCATCGAAAAAAACGAAAGCGCCGAGCAAAAAACAAAAAACGGCCCGCAAAAAACAGCAAATGGAACCGGAGCCTTCTGCTGAAGAGGGGGATTTGGAAACGGTATc                                                             >  1:1712503/1‑140 (MQ=255)

                                                                                   tgatATGAAATCCGTCAAAACCAATATGTAGTCATCTAAAAAAACGAAAGCGCCGAGAAAAAAACAAAAAACGGCCCGCAAAAAACAGCAAATGGAACCGGAGCCTTCTGCTGAAGAGGGGGATTTGGAAACGGTATCGc                                                           <  2:2679190/140‑1 (MQ=255)

                                                                                     atatGAAATCCGTCAAAACCAATATTAAGTCATCTAAAAAAACGAAAGCCCCGAGCAAAAAACAAAAAACGGCCCGCAAAAAACAGCAAATGGAACCGGAGCCTTCTGCTGAAGAGGGGGATTTGGAAACGGTATCGCct                                                         <  2:1712503/140‑1 (MQ=255)

                                                                                     atatGAAATCCGACAAAACCAATATGAAGTCATCGAAAAAAACGAAAGCGCCGAGCAAAAAACAAAAAACGGCCCGCAACAAACAGCAAATGGAACCGGAGCCTTCTGCTGAAGAGGGGGATTTGGAAACGGTATCGCct                                                         <  2:2906236/140‑1 (MQ=255)

                                                                                      tatGAAATCCGTCAAAACCAATATGAAGTCATCGAAAAAAACGAAAGCGCCGAGCAAAAAACAAAAAACGGCCCGCAAAAAACAGCAAATGGAACCGGAGCCTTCTGCTGAAGAGGGGGATTTGGACACGGTATCGCctc                                                        >  1:5871297/1‑140 (MQ=255)

                                                                                       atGAAATCCTTCAAAACCAATATGAAGTCATCTAAAAAAACGAAAGCGCCGAGCAAAAAACAAAAAACGGCCCGCAAAAAACAGCAAATGGAACCGGAGCCTTCTGCTGAAGAGGGGGATTTGGAAACGGTATCGCctct                                                       <  2:4947096/140‑1 (MQ=255)

                                                                                          aaaTCCGTCAAAACCAATATGAATTCATCTAAAAAAACGAAAGCGCCGAGCAAAAAACAAAAAACGGCCCGCAAAAAACAGCAAATGGAACCGGAGCCTTCTGCTGAAGAGGGGGATTTGGAAACGGTATCGCCTCTTat                                                    <  1:1608259/140‑1 (MQ=255)

                                                                                          aaaTCCGTCAAAACCAATATGAAGTCATCTAAAAAAACGAAAGCGCCGAGCAAAAAACAAAAAACGGCCCGCAAAAAACAGCAAATGGAACCGGAGCCTTCTGCTGAAGAGGGGGATTTGGAAACGGTATCGCCTCTTat                                                    <  2:7471015/140‑1 (MQ=255)

                                                                                          aaaGCCGTCAAAACAAATATGAATTCATCGAAAAAAACTAACGCGCCGAGAAAAAAACAAAAAACGGCCCGCAAAAAACAGCAAATGGAACCGGAGCCTTCTGCTGAAGAGGGGGATTTGGAAACGGTATCGCCTCTTat                                                    <  2:2026552/140‑1 (MQ=255)

                                                                                                 tCAAAACCAATATGAAGTCATCGAAAAAAACGAAAGCGCCGAGCAAAAAACAAAAAACGGCCCGCAAAAAACAGCAAATGGAACCTGATCCTTATGCTGAAGAGGGGGATTTGGAAACGGTATCCACTCTTAttaagtca                                             >  2:6088217/1‑140 (MQ=255)

                                                                                                 tCAAAACCAATATGAAGTCATCGAAAAAAACGAAAGCGCCGAGCAAAAAACAAAAAACGGCCCGCAAAAAACAGCAAATGGAACCGGAGCCTTCTGCTGAAGAGGGGGATTTGGAAACGGTATCGCCTCTTAttcattca                                             >  2:828281/1‑140 (MQ=255)

                                                                                                 tCAAAACCAATATGAAGTCATCGAAAAAAACGAAAGCGCCGAGCAAAAAACAAAAAACGGCCCGCAAAAAACAGCAAATGGAACCGGAGCCTTCTGCTGAAGAGGGGGATTTGGAAACGGTATCGCCTCTTAttcattca                                             >  1:3383369/1‑140 (MQ=255)

                                                                                                 tCAAAACCAATATGAAGTCATCGAAAAAAACGAAAGCGCCGAGCAAAAAACAAAAAACGGCCCGCAAAAAACAGCAAATGGAACCGGAGCCTTCTGCTGAAGAGGGGGATTTGGAAACGGTATCGCCTCTTAttcattca                                             >  2:4144001/1‑140 (MQ=255)

                                                                                                 tCAAAACCAATATGAAGTCATCGAAAAAAACGAAAGCGCCGAGCAAAAAACAAAAAACGGCCCGCAAAAAACAGCAAATGGAACCGGAGCCTTCTGCTGAAGAGGGGGATTTGGAAACGGTATCGCCTCTTAttcattca                                             >  2:1543963/1‑140 (MQ=255)

                                                                                                 tCAAAACCAATATGAAGTCATCGAAAAAAACGAAAGCGCAGAGCAAAAAACAAAAAACCGCCCGCAAAAAACAGCAAATGGAACCGGAGCCTTCTGCTGAAGAGGGGGATTTGGAAACGGTATCGCCTCTTAttcattca                                             >  1:3201338/1‑140 (MQ=255)

                                                                                                    aaaCAAATATGAAGTCATCGAAAAAAACGAAAGCGCCGAGCAAAAAACAAAAAACGGCCCGCAAAAAACAGCAAATGGAACCGGAGCCTTCTGCTGAAGAGGGGGATTTGGAAACGGTATCGCCTCTTATTCATTCagag                                          <  2:1134474/140‑1 (MQ=255)

                                                                                                        cAATATTAAGTCATCTAAAAAAACGAAAGCGCCGAGCAAAAAACAAAAAACGGCCCGCAAAAAACAGCAAATGGAACCGGAGCCTTCTGCTGAAGAGGGGGATTTGGAAACGGTATCGCCTCTTATTCATTCAGAGCCGa                                      <  1:3583973/140‑1 (MQ=255)

                                                                                                          atatGAAGTCATCGAAAAAAACGAAAGCGCCGAGCAAAAAACAAAAAACGGCCCGCAAAAAACAGCAAATGGAACCGGAGCCTTCTGCTGAAGAGGGGGATTTGGAAACGGTATCGCCTCTTATTCATTCAGAGCCGAtt                                    >  2:5969015/1‑140 (MQ=255)

                                                                                                             tGAAGTCATCGAAAAAAACGAAAGCGCCGAGCAAAAAACAAAAAACGGCCCGCAAAAAACAGCAAATGGAACCGGAGCCTTCTGCTGAAGAGGGGGATTTGGAAACGGTATCGCCTCTTATTCATTCAGAGCCGATTAtc                                 <  2:2274396/140‑1 (MQ=255)

                                                                                                                 gTCATCGAAAAAAACGAAAGCGCCGAGCCAAAAACAAAAAACGGCCCGCAAAAAACAGCAAATGGAACCGGAGCCTTCTGCTGAAGAGGGGGATTTGGAAACGGTATCGCCTCTTATTCATTCAGAGCCGATTATCTaaa                             >  2:3989605/1‑140 (MQ=255)

                                                                                                                  tCATCGAAAAAAACGAAAGCGCCGAGCAAAAAACAAAAAACGGCCCGCAAAAAACAGCAAATGGAACCGGAGCCTTCTGCTGAAGAGGGGGATTTGGAAACGGTATCGCCTCTTATTCATTCATAGCCGATTATCTCAAg                            >  2:3930502/1‑140 (MQ=255)

                                                                                                                  tCATCGAAAAAAACGAAAGCGCCGAGCAAAAAACAAAAAACGGCCCGCAAAAAACAGCAAATGGAACCGGAGCCTTCTGCTGAAGAGGGGGATTTGGAAACGGTATCGCCTCTTATTCATTCAGAGCCGATTATCTCAAg                            >  2:1035427/1‑140 (MQ=255)

                                                                                                                    aTCGAAAAAAACGAAAGCGACTATAAAAAAAAAAAAAAATTAAATCAAAAAAAAGCAAATGGAACCGGAGCCTTCTGCTGAAGAGGGGGATTTGGAAACGGTATCGCCTCTTATTCATTCAGAGCCGATTATCTCAAGCt                          >  2:6359201/1‑140 (MQ=255)

                                                                                                                     tCGAAAAAAACGAAAGCGCCGAGCAAAAAACAAAAAACGGCCCGCAAAAAACAGCAAATGGAACCGGAGCCTTCTGCTGAAGAGGGGGATTTGGAAACGGTATCGCCTCTTATTCATTCAGAGCCGATTATCTCAAGCtt                         >  2:3263720/1‑140 (MQ=255)

                                                                                                                         aaaaaaCGAAAGCGCCGAGCAAAAAACAAAAAACGGCCCGCAAAAAACAGCAAATGGAACCGGAGCCTTCTGCTGAAGAGGGGGATTTGGAAACGGTATCGCCTCTTATTCATTCAGAGCCGATTATCTCAAGCTTTTCa                     >  1:7164579/1‑140 (MQ=255)

                                                                                                                                 aaaGCGCCGAGCAAAAAACAAAAAACGGCCCGCAAAAAACAGCAAATGGAACCGGAGCCTTCTGCTGAAGAGGGGGATTTGGAAACGGTATCGCCTCTTATTCATTCAGAGCCGATTATCTCAAGCTTTTCAGATCGTaa             <  2:1833991/140‑1 (MQ=255)

                                                                                                                                 aaaGCGCCGAGCAAAAAACAAAAAACGGCCCGCAAAAAACAGCAAATGGAACCGGAGCCTTCTGCTGAAGAGGGGGATTTGGAAACGGTATCGCCTCTTATTCATTCAGAGCCGATTATCTCAAGCTTTTCAGATCGTaa             <  2:2495609/140‑1 (MQ=255)

                                                                                                                                   cgcgcCGAGAAAAAAACAAAAAACGGCCCGCAAAAAACAGCAAATGGAACCGGAGCCTTCTGCTGAAGAGGGGGATTTGGAAACGGTATCGCCTCTTATTCATTCAGAGCCGATTATCTCAAGCTTTTCAGATCGTAatg           <  1:6927151/139‑1 (MQ=255)

                                                                                                                                   aGCGCCGAGCAAAAAACAAAAAACGGCCCACAAAAAACAGCAAATGGAACCGGAGCCTTCTGCTGAAGAGGGGGATTTGGAAACGGTATCGCCTCTTATTCATTCAGAGCCGATTATCTCAAGCTTTTCAGATCGTAatg           <  1:7605458/140‑1 (MQ=255)

                                                                                                                                      gcCGAGCAAAAAACAAAAAACGGCCCGCAAAAAACAGCCCATCGCACCGGAGCCTTCTGCTGAAGAGGGGGATTTGGAAACGGTATCGCCTCTTATTCAGTCAGAGCCGATTATCTCAAGCTTTTCAGATCGTAatgaag        <  1:1527727/140‑1 (MQ=255)

                                                                                                                                      gcCGAGCAAAAAACAAAAAACGGCCCGCAAAAAACAGCAAATGGAACCGGAGCCTTCTGCTGAAGAGGGGGATTTGGAAACGGTATCGCCTCTTATTCATTCAGAGCCGATTATCTCAAGCTTTTCAGATCGTAatgaag        <  1:4669177/140‑1 (MQ=255)

                                                                                                                                      gcCGAGCAAAAAACAAAAAACGGCCCGCAAAAAACAGCAAATGGAACCGGAGCCTTCTGCTGAAGAGGGGGATTTGGAAACGGTATCGCCTCTTATTCATTCAGAGCCGATTATCTCAAGCTTTTCAGATCGTAatgaag        <  1:2969661/140‑1 (MQ=255)

                                                                                                                                        ccaGCAAAAAACAAAAAACGGCCCGCAAAAAACATCAAATGGAACCGGAGCCTTCTGCTGAAGAGGGGGATTTGGAAACGGTATCGCCTCTTATTCATTCAGAGCCGATTATCTCAAGCTTTTCAGATCGTAatgaagat      <  2:3531117/138‑1 (MQ=255)

                                                                                                                                          atcaaaaaacaaaaaaCGGCCCGCAAAAAACAGCAAATGGAACCGGAGCCTTCTGCTGAAGAGGGGGATTTGGAAACGGTATCGCCTCTTATTCATTCAGAGCCGATTATCTCAAGCTTTTCAGATCGTAatgaagatga    <  2:4847466/138‑1 (MQ=255)

                                                                                                                                          aGCAAAAAACAAAAAACGGCCCGCAAAAAACATCAAATGGAACCGGAGCCTTCTGCTGAAGAGGGGGATTTGGAAACGGTATCGCCTCTTATTCATTCAGAGCCGATTATCTCAAGCTTTTCAGATCGTAatgaagatga    <  2:3190989/140‑1 (MQ=255)

                                                                                                                                           ccaaaaaacaaaaaaCGGCCCGCAAAAAACAGCAAATGGAACCGGAGCCTTCTGCTGAAGAGGGGGATTTGGAAACGGTATCGCCTCTTATTCATTCAGAGCCGATTATCTCAAGCTTTTCAGATCGTAatgaagatgaa   <  1:656797/139‑1 (MQ=255)

GCGCTCTCTTCAAGAAACGCTGAAAAAGTGGATGAGCCCGATTGGCCGCTTTATAAAAGAACAATGG‑TTT‑GCATTTATTGATGATATGAAATCCGTCAAAACCAATATGAAGTCATCGAAAAAAACGAAAGCGCCGAGCAAAAAACAAAAAACGGCCCGCAAAAAACAGCAAATGGAACCGGAGCCTTCTGCTGAAGAGGGGGATTTGGAAACGGTATCGCCTCTTATTCATTCAGAGCCGATTATCTCAAGCTTTTCAGATCGTAATGAAGATGAAG  >  NZ_CP034943/1720610‑1720887

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`