BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	2,214,518	T→G	6.5%	K60T (AAA→ACA)	nth ←	endonuclease III

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	2,214,518	0	T	G	6.5%	1043.4 / 9.3	432	K60T (AAA→ACA)	nth	endonuclease III
Reads supporting (aligned to +/- strand): ref base T (78/305); new base G (37/12); total (115/317)
Fisher's exact test for biased strand distribution p-value = 2.66e-14
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 462 total aligned reads displayed.

CGGCACTTCTCCGCCGTAATCTTCAATAATCATTTTGCTCAGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGT‑CACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAACCAGTTCACACTCTGCGTGTGGAAACATGTCGCCTAT  >  NZ_CP034943/2214379‑2214657

     cTTCTCCGCCGTTATCTTCAATAATCATTTTGCTCAGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTatat                                                                                                                                         <  2:3948894/140‑1 (MQ=255)

       tctcCGCCGTAATCTTCAATAATCATTTTGCTCAGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATAttt                                                                                                                                       >  1:1003225/1‑140 (MQ=255)

       tctcCGCCGTAATCTTCAATAATCATTTTGCTCAGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATAttt                                                                                                                                       <  1:7288919/140‑1 (MQ=255)

             ccgTAATCTTCAATAATCATTTTGCTCAGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTGTTTATTTTTTaaa                                                                                                                                 >  1:2918798/1‑140 (MQ=255)

               gTAATCTTCAATCATCATTTTGCTCAGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAaata                                                                                                                               <  2:7477869/140‑1 (MQ=255)

               gTAATCTTCAATAATCATTTTGCTCAGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAaata                                                                                                                               <  2:1105967/140‑1 (MQ=255)

               gTAATCTTCAATAATCATTTTGCTCAGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAaata                                                                                                                               <  2:1716640/140‑1 (MQ=255)

                   tCTTCAATCATCATTTTGCTCAGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGt                                                                                                                           <  2:6270818/140‑1 (MQ=255)

                     ttCAATAATCATTTTGCTCAGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTTAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGttt                                                                                                                         <  2:5870366/140‑1 (MQ=255)

                     ttCAATAATCATTTTGCTCAGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGttt                                                                                                                         >  2:410740/1‑140 (MQ=255)

                          taatCATTTTGCTCAGTTTTTGGATGATTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGT‑c                                                                                                                   <  2:1287525/140‑1 (MQ=255)

                            atCATTTTGCTCAGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGT‑CAc                                                                                                                 <  1:5716703/140‑1 (MQ=255)

                            atCATTTTGCTCAGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGT‑CAc                                                                                                                 <  1:4156393/140‑1 (MQ=255)

                            atCATTTTGCTCAGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGT‑CAc                                                                                                                 <  1:1914087/140‑1 (MQ=255)

                             tCATTTTGCTCAGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGT‑CAct                                                                                                                <  1:3048211/140‑1 (MQ=255)

                             tCATTTTGCTCAGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGT‑CAct                                                                                                                <  1:7652574/140‑1 (MQ=255)

                             tCATTTTGCTCAGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGT‑CAct                                                                                                                <  1:2212381/140‑1 (MQ=255)

                             tCATTTTGCTCAGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGT‑CAct                                                                                                                <  1:2017093/140‑1 (MQ=255)

                             tCATTTTGCTCAGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGT‑CAct                                                                                                                <  2:7680191/140‑1 (MQ=255)

                             tCATTTTGCTCAGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGT‑CAct                                                                                                                <  2:2199051/140‑1 (MQ=255)

                             tCATTTTGCTCAGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGT‑CAct                                                                                                                <  1:4604929/140‑1 (MQ=255)

                             tCATTTTGCTCAGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGT‑CAct                                                                                                                <  1:6375419/140‑1 (MQ=255)

                                     cTCAGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGT‑CACTCTGTTTAc                                                                                                        <  1:952358/140‑1 (MQ=255)

                                        aGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGT‑CACTCTGTTTACAAg                                                                                                     <  2:4828276/140‑1 (MQ=255)

                                        aGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGT‑CACTCTGTTTACAAg                                                                                                     <  2:2251932/140‑1 (MQ=255)

                                        aGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGT‑CACTCTGTTTACAAg                                                                                                     <  2:5543508/140‑1 (MQ=255)

                                        aGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGT‑CACTCTGTTTACAAg                                                                                                     <  2:1943950/140‑1 (MQ=255)

                                        aGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGT‑CACTCTGTTTACAAg                                                                                                     <  2:3685942/140‑1 (MQ=255)

                                        aGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGT‑CACTCTGTTTACAAg                                                                                                     <  1:4405585/140‑1 (MQ=255)

                                        aGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATAATTTTGAAATAATGTTTTGGT‑CACTCTGTTTACAAg                                                                                                     <  1:2675389/140‑1 (MQ=255)

                                         gTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGT‑CACTCTGTTTACAAGt                                                                                                    <  1:6345976/140‑1 (MQ=255)

                                          tttttGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGT‑CACTCTGTTTACAAGTg                                                                                                   <  2:297905/140‑1 (MQ=255)

                                          tttttGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGT‑CACTCTGTTTACAAGTg                                                                                                   <  2:1440991/140‑1 (MQ=255)

                                          tttttGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGT‑CACTCTGTTTACAAGTg                                                                                                   <  2:4588997/140‑1 (MQ=255)

                                          tttttGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGT‑CACTCTGTTTACAAGTg                                                                                                   <  2:2061516/140‑1 (MQ=255)

                                          tttttGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGT‑CACTCTGTTTACAAGTg                                                                                                   <  1:6200911/140‑1 (MQ=255)

                                          tttttGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGT‑AAATCTGTTTACAAGTg                                                                                                   >  1:3857582/1‑140 (MQ=255)

                                          tttttGGATGTTTTTCGCTTTAGTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGT‑CACTCTGTTTACAAGTg                                                                                                   <  2:7588711/140‑1 (MQ=255)

                                          tttttGGATGTTTGTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGT‑CACTCTGTTTACAAGTg                                                                                                   <  2:5358531/140‑1 (MQ=255)

                                          tttttGGATGATTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGT‑CACTCTGTTTACAAGTg                                                                                                   <  1:5423608/140‑1 (MQ=255)

                                             ttGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGT‑CACTCTGTTTACAAGTGCAt                                                                                                <  2:6576275/140‑1 (MQ=255)

                                                    tttttCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTTGT‑CACTCTGTTTAAAAGTGCATCTTTACa                                                                                         >  2:4199553/1‑140 (MQ=255)

                                                         cGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGT‑CACTCTGTTTACAAGTGCATCTGTACATTgcg                                                                                    <  2:47752/140‑1 (MQ=255)

                                                         cGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGT‑CACTCTGTTTACAAGTGCATCTGTACATTgcg                                                                                    <  1:2403000/140‑1 (MQ=255)

                                                         cGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGT‑CACTCTGTTTACAAGTGCATCTGTACATTgcg                                                                                    <  2:3799803/140‑1 (MQ=255)

                                                         cGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTATTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGT‑CACTCTGTTTACAAGTGCATCTGTACATTgcg                                                                                    <  2:6199208/140‑1 (MQ=255)

                                                                      tACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTTTATTTTTGAAATAAAGTTTTGGT‑GACTCTGTTTAAAAGTGCATCTGTACCTTTCGCAGATAAATCGAc                                                                       >  1:4732857/1‑140 (MQ=255)

                                                                          aaCCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGT‑CACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCt                                                                   <  2:68434/140‑1 (MQ=255)

                                                                               attgattTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTGTTTATTTTTTTAAAAAATTTTTTGT‑GACTATGTTTAAAAATGCATCTGTACAATTCGCAGATAAAGCGACTGCTAacac                                                              >  1:5951457/1‑140 (MQ=255)

                                                                                ttgattTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGT‑CACTCTGTTTACAAGTg                                                                                                   <  1:6464854/102‑1 (MQ=255)

                                                                                ttgattTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGT‑CACTCTGTTTACAAGTg                                                                                                   >  2:6464854/1‑102 (MQ=255)

                                                                                ttgattTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTGTTTATTTTTTTAATAATTTTTTTGT‑GACTCTGTTTAAAAGTGGAACTGTAAAATTCGCAGATAAATCGACTGCTACCAca                                                             >  1:874479/1‑139 (MQ=255)

                                                                                ttgattTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCCGCCGTTTATATTTTTGAAAAAAAGTTTTTGT‑AAATATTTTTACAAATGCAACTGTAAAATGCGAAAATAAACCGACTGATAacaca                                                             >  2:4337487/1‑139 (MQ=255)

                                                                                       aatttgctgctgAAGCTCTACCAGCGAAACAGCAAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGT‑CACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCaa                                                      <  1:7034557/134‑1 (MQ=255)

                                                                                       aaTATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGT‑CACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCaa                                                      <  1:2108925/140‑1 (MQ=255)

                                                                                             ctgctgAAGCTCTTCCATCGAAACATCCAAATAGTCTTCCGGCCGTGTATATTTTTGAAATAATGTTTTGCT‑CACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGAt                                                <  2:2114828/140‑1 (MQ=255)

                                                                                             ctgctgAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGT‑CACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGAt                                                <  1:5230129/140‑1 (MQ=255)

                                                                                                  gAATCTCTTCCAGCGAAACATCCAAATAGTCTTCCTGCCGTTTATTTTTTTGATTTAATTTTTTGGT‑CACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAAGTCAAAAGGATTGGaa                                           <  2:1833361/140‑1 (MQ=255)

                                                                                                   aaGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGT‑CACTCTGTTTACAAGt                                                                                                    <  1:6727439/82‑1 (MQ=255)

                                                                                                   aaGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGT‑CACTCTGTTTACAAGt                                                                                                    <  1:4968489/82‑1 (MQ=255)

                                                                                                   aaGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGT‑CACTCTGTTTACAAGt                                                                                                    >  2:6727439/1‑82 (MQ=255)

                                                                                                   aaGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGT‑CACTCTGTTTACAAGt                                                                                                    <  1:3942723/82‑1 (MQ=255)

                                                                                                     gCTCTTCCAGCGAAACAGCCCAATAGTCTTCCGGCCGTTTATATTTTTTAAATAATTTTTTGGT‑CACTCGGGTTACAAGTGCATCTTTACATTGCGCACATAAAGCGACTGCTACCACTAATTCAAAAGCATTGGAATGa                                        >  2:5157717/1‑140 (MQ=255)

                                                                                                      ctctTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGT‑CACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGaa                                       <  2:1971850/140‑1 (MQ=255)

                                                                                                      ctctTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGT‑CACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGaa                                       <  1:3544577/140‑1 (MQ=255)

                                                                                                      ctctTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGT‑CACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGaa                                       <  1:7710100/140‑1 (MQ=255)

                                                                                                      ctctTCCAGCGAAACAGCAAAATAGTCTTCCGGCCTTTTATATTTTTGAAATAATGTTTTGGT‑CACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGaa                                       <  1:4550602/140‑1 (MQ=255)

                                                                                                      ctctTCCAGAGAAACATCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTGGGT‑CAGTGTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGaa                                       <  2:7115543/140‑1 (MQ=255)

                                                                                                        ctgccACCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGT‑CACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAAcc                                     <  1:1111337/137‑1 (MQ=255)

                                                                                                        ctTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTTAAATAATGTTTTGGT‑CACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAAcc                                     <  1:4199553/140‑1 (MQ=255)

                                                                                                        ctTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGT‑CACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAAcc                                     <  1:7447938/140‑1 (MQ=255)

                                                                                                        agtCCAGCGAAACAGCCAAATAGACTTCCGGCCGTGTCGATGTTTGAAATACTGTTTTGGT‑CACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAAcc                                     <  2:4946044/138‑1 (MQ=255)

                                                                                                          tCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGT‑CAct                                                                                                                >  2:4506090/1‑63 (MQ=255)

                                                                                                          tCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGT‑CACTCTGTTTACAAGTGCATCTGTACa                                                                                         <  1:1419542/86‑1 (MQ=255)

                                                                                                          tCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTGTTTTTTTTTTTAATAATTTTTTTGTGCAGCTTGTTTA‑AAATGGCTCTGTACAATTGGCAGATAAAGAGGCTGCTACCACAAATTGAAAAGGATTGGTATTaaaccg                                   >  1:3385852/1‑136 (MQ=255)

                                                                                                            cAGCGAAACAGCCAAATAGTCGTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGT‑CACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAACCAGtt                                 <  2:5717659/140‑1 (MQ=255)

                                                                                                            cAGCGAAACAGCAAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGT‑CACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAACCAGtt                                 <  1:3751164/140‑1 (MQ=255)

                                                                                                            cAGCGAAACAGCAAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGT‑CACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAACCAGtt                                 <  2:7235217/140‑1 (MQ=255)

                                                                                                             aGCGAAACAGCCAAATAGTCTTCCGGCCGTGTTTATTTTTTGAATAAAGTTTTGGT‑GACTCTGTTTTCAATTGCATCTGTACAGTTGGCCGATAAAGAGACTGCTACCACAAAATAAAAAGGATTTGCATTAACCAggta                                >  1:1656754/1‑137 (MQ=255)

                                                                                                              gCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGT‑CACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAACCAGTTca                               <  2:3307214/140‑1 (MQ=255)

                                                                                                              gCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGT‑CACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAACCAGTTca                               <  2:50234/140‑1 (MQ=255)

                                                                                                                gAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGT‑CACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAACCAGTTcaca                             <  2:3430929/140‑1 (MQ=255)

                                                                                                                     aGCCAAATAGTCTTCCGGCCGTTTATAGTTTTGAAATAATGTTTTGGT‑CACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAACCAGTTCACACTCTg                        <  2:4031389/140‑1 (MQ=255)

                                                                                                                             aGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGT‑CACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAACCAGTTCACACTCTGCGTGTGGa                <  2:6969424/140‑1 (MQ=255)

                                                                                                                               tCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGT‑CACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCCCTAATTCAAAAGGATTGGAATGAACCAGTTAACACTCTGCGTGTGGaaa              >  2:3071257/1‑140 (MQ=255)

                                                                                                                                    cGGCCGTTTATATTTTTGTAATAATGTTTTGGT‑CACTCTGTTTACAACTGCAACAGTACAAGGCGCAGATAAATCGACTTCTACCACTAATTAAAAAGGATTGGAAAGAACCACTTCACACACTGCGTGTGGCAACAAGt         >  2:2430533/1‑140 (MQ=255)

                                                                                                                                      gCCGTTTATATTTTTGAAAAAATGTTTTGGT‑AACTCTGTTTACAATTGCATCAGTACATTGCGCAGATAAAACGACTGCTCCCACAAATTCAAAAGGATTGGAATGAAACATGTCACACAATGCGTGTGGAAACATGTCg       >  2:799821/1‑140 (MQ=255)

                                                                                                                                       ccGTTTATATTTTTTAAATATTGTTTTGGT‑CAATCTGTTTACAAGTGCATCTGTACATTGCGGAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAACCAGTTCACACTCTGCGTGTGGAAACATGTCGc      <  2:3176341/140‑1 (MQ=255)

                                                                                                                                       ccGTTTATATTTTTGAAATAATGTTTTGGT‑CACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAACCAGTTCACACTCTGCGTGTGGAAACATGTCGc      <  2:3752985/140‑1 (MQ=255)

                                                                                                                                       ccGTTTATATTTTTGAAATAATGTTTTGGT‑CACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAACCAGTTCACACTCTGCGTGTGGAAACATGTCGc      <  2:5951457/140‑1 (MQ=255)

                                                                                                                                         gTTTATATTTTTGTAAAAAAGTTTTTGT‑CACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTGAAAAGGATTTGAATGAACCAGTTAACACACTGAGTGTGGAAAAATGTCGcca    >  2:6056482/1‑139 (MQ=255)

                                                                                                                                           ttATCTTTTTTAAATATTGTTTTTGT‑CACTCTTTTTACAAGTGCATATGTAATTTGCGCAGATAAAGCGACTGCTACCACTACTTCAAAAGGATTGGAATGAACCAGTTCACCCTCTGCGTGTGGAAACATGTCGCCTAt  <  1:6607832/140‑1 (MQ=255)

                                                                                                                                           ttATATTTTTGAAATAATGTTTTGGT‑CACTCTGTTTACAAGTGCATCTGTACATTGTGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAACCAGTTCACACTCTGCGTGTGGAAACATGTCGCCTAt  <  2:5590908/140‑1 (MQ=255)

                                                                                                                                           ttATATTTTTGAAATAATGTTTTGGT‑CACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAACCAGTTCACACTCTGCGTGTGGAAACATGTCGCCTAt  <  1:5062195/140‑1 (MQ=255)

                                                                                                                                           ttATATTTTTGAAATAATGTTTTGGT‑CACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAACCAGTTCACACTCTGCGTGTGGAAACATGTCGCCTAt  <  1:4841782/140‑1 (MQ=255)

                                                                                                                                           ttATATTTTTGAAATAATGTTTTGGT‑CACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAACCAGTTCACACTCTGCGTGTGGAAACATGTCGCCTAt  <  1:5352321/140‑1 (MQ=255)

                                                                                                                                           ttATATTTTTGAAATAATGTTTTGGT‑CACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAACCAGTTCACACTCTGCGTGTGGAAACATGTCGCCTAt  <  1:5479184/140‑1 (MQ=255)

                                                                                                                                           ttATATTTTTGAAATAATGTTTTGGT‑CACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAACCAGTTCACACTCTGCGTGTGGAAACATGTCGCCTAt  <  1:3124697/140‑1 (MQ=255)

                                                                                                                                           ttATATTTTTGAAATAATGTTTTGGT‑CACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAACCAGTTCACACTCTGCGTGTGGAAACATGTCGCCTAt  <  2:2867703/140‑1 (MQ=255)

                                                                                                                                           ttATATTTTTGAAATAATGTTTTGGT‑CACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGATACCACTAATTCAAAAGGATTGGAATGAACCAGTTCACACTCTGCGTGTGGAAACATGTCGCCTAt  <  2:1574993/140‑1 (MQ=255)

CGGCACTTCTCCGCCGTAATCTTCAATAATCATTTTGCTCAGTTTTTGGATGTTTTTCGCTTTATTACGATACAAACCGATTGATTTAATATCCTGCTGAAGCTCTTCCAGCGAAACAGCCAAATAGTCTTCCGGCCGTTTATATTTTTGAAATAATGTTTTGGT‑CACTCTGTTTACAAGTGCATCTGTACATTGCGCAGATAAAGCGACTGCTACCACTAATTCAAAAGGATTGGAATGAACCAGTTCACACTCTGCGTGTGGAAACATGTCGCCTAT  >  NZ_CP034943/2214379‑2214657

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`