BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	3,219,805	G→T	11.8%	K381N (AAG→AAT)	oxdC →	oxalate decarboxylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	3,219,805	0	G	T	11.8%	591.4 / 7.3	310	K381N (AAG→AAT)	oxdC	oxalate decarboxylase
Reads supporting (aligned to +/- strand): ref base G (152/69); new base T (3/43); total (155/155)
Fisher's exact test for biased strand distribution p-value = 9.73e-16
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 312 total aligned reads displayed.

AAAGACGATCATTATGCCGATGTATCTTTAAACCAATGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGT‑TGT‑AAAAAAGAAATGCAGTAAATAAAA‑AACGACCAGCTTGCAGAGAGCAATCGTTCTCTGCAAGCCTTTATAAGGAGATGTACCAGTGAAACATCAAATTTCTTCAAAACGCTTATTGCGGCTCTTTATAAAGTACTCGCTGGCCGCGA  >  NZ_CP034943/3219668‑3219944

       aTCATTATGCCGATGTATCTTTAAACCAATGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGT‑TGT‑AAAAAAGAAATGCAGt                                                                                                                                     >  1:7181461/1‑140 (MQ=255)

       aTCATTATGCCGATGTATCTTTAAACCAATGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGT‑TGT‑AAAAAAGAAATGCAGt                                                                                                                                     >  1:7548194/1‑140 (MQ=255)

              ttccGATGTATCTTTAAACCAATGGCTTGCAATGCTTCCTGAAACATTAGTTAAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGT‑TGT‑AAAAAATAAATGCAGaaaataaa                                                                                                                              >  1:1130064/3‑140 (MQ=255)

                ccGATGTATCTTTAAACCAATGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGT‑TGT‑AAAAAAGAAATGCAGTAAATaaaa‑a                                                                                                                           >  2:2859973/1‑140 (MQ=255)

                ccGATGTATCTTTAAACCAATGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGT‑TGT‑AAAAAAGAAATGCAGTAAATaaaa‑a                                                                                                                           >  1:6481048/1‑140 (MQ=255)

                ccGATGTATCTTTAAACCAATGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGT‑TGT‑AAAAAAGAAATGCAGTAAATaaaa‑a                                                                                                                           >  2:7107959/1‑140 (MQ=255)

                   aTGTATCTTTAAACCAATGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGT‑TGT‑AAAAAAGAAATGCAGTAAATAAAA‑AACg                                                                                                                        >  1:3347426/1‑140 (MQ=255)

                   aTGTATCTTTAAACCAATGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGT‑TGT‑AAAAAAGAAATGCAGTAAATAAAA‑AACg                                                                                                                        >  1:3481680/1‑140 (MQ=255)

                   aTGTATCTTTAAACCAATGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGT‑TGT‑AAAAAAGAAATGCAGTAAATAAAA‑AACg                                                                                                                        >  2:7198118/1‑140 (MQ=255)

                    ttttattttACACCATTGGGTTTAATTGTTTCCTTATACTTTATTTAAAAAGAATCTTTATTTTGTAAAACATTTTACTTATATTCTTTCTAAATAAAAGCATCGGGT‑TGT‑AAAAAAGAAATGCATTAAATAAAA‑AACGa                                                                                                                       <  2:4594555/134‑1 (MQ=255)

                    ttttacTTTAAACCAATGGTTTGCAATTCTTCCTCAAACATTATTTAAACAGCATATTGATTTAGTAAAAGACTTTACTGATTTTCTTTAGAAAGAAAAGCATCCGGT‑TGT‑AAAAAAGAAATGCATTAATTAAAA‑AACGa                                                                                                                       <  2:5685455/135‑1 (MQ=255)

                    tttataTTTAAACCTGTGTCTTTCAATGTTTCCTGAAACATTAGTTAAACATCTTCTTTATTTAGGAAAATACTTTACTGATATTCTTTCTAAATAAAAGAATCCGTT‑TGT‑AAAAAATAAATGCAGTAAATAAAA‑AACGa                                                                                                                       <  2:4683632/138‑1 (MQ=255)

                    tttataTTTAAACAATTGGCTTGCAATGGTTCCTGAAACTTTATTTCAACAGCATCTTGATTTAGTAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGT‑TGT‑AAAAAATAAATCCAGTAAATAAAA‑AACGa                                                                                                                       <  2:1704515/138‑1 (MQ=255)

                    tGTATCTTTAAACCAATGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGT‑TGT‑AAAAAAGAAATGCAGTAAATAAAA‑AActa                                                                                                                       >  1:6268620/1‑138 (MQ=255)

                    tGTATCTTTAAACCAATGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGT‑TGT‑AAAAAAGAAATGCAGTAAATAAAA‑AACGa                                                                                                                       >  2:4391307/1‑140 (MQ=255)

                    tGTATCTTTAAACCAATGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGT‑TGT‑AAAAAAGAAATGCAGTAAATAAAA‑AACGa                                                                                                                       >  1:4696172/1‑140 (MQ=255)

                    tGTATATTTAAACCAAGGGCTTGAAATGGTGCCTCAAACATTTGTAAAACAGAATGTTTATTTCGCAAAACACTTTACTGATATTCTTTCGAAACAAAAGCATCCGGT‑TGA‑AAAAAAGAAAGGCATTAAATAAAA‑CACGa                                                                                                                       <  2:2663505/140‑1 (MQ=255)

                        tCTTTAAACCAATGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGT‑TGT‑AAAAAATAAATGCAGTAAATAAAA‑AACGACCAg                                                                                                                   >  2:3045387/1‑140 (MQ=255)

                        tCTTTAAACCAATGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGT‑TGT‑AAAAAAGAAATGCAGTAAATAAAA‑AACGACCAg                                                                                                                   >  2:7000027/1‑140 (MQ=255)

                         ttttAAACCATGGGCTTGCATTGCGTCCTCAAACTTTAGTTCACCAGAATTTTGTTTTAGGAAAAGATTTTACTTATTTTCTTTCCAAAGAAAAGCATCCGTT‑TGT‑AAAAAACAAAGGCATTAAATAAAA‑AACGACCAGc                                                                                                                  <  2:2560521/139‑1 (MQ=255)

                              accccctGGCTTCAAAGGCTTCCTCAAACATTATTACCACACCATTTTGATTGAGGAAAAGACTTTACTCATATTCTTACGAACCAAAAGCACCCGTT‑TTT‑AAAAACTAAATGCCGAAAATAAAA‑AACGACCAGCTTGCa                                                                                                             <  2:3095479/134‑1 (MQ=255)

                              aaCCATTGGTTTGAAATGCTTCCTTAAATATTAGTACAACAGCATCTTTATTTAGCAAAATATTTTACTGATATTCTTTCCAAATAAAAGCATCCGTT‑TTT‑AAAAAAAAAATGCATTAAATAAAA‑AACGACCAGCTTGCa                                                                                                             <  2:4832304/140‑1 (MQ=255)

                              aaCCATTGGCTTTAAATTCTTCCTTAAACTTTATTAAAAAAGAATCTTGATTTAGTAAAATACTTTACTCATATTCTTCAGAATCAAAACCATCGGGT‑TTT‑AAAAAACAAATCAATTAAATAAAA‑AACGACCAGCTTGCa                                                                                                             <  2:2759606/140‑1 (MQ=255)

                              aaCCATGGGATTTCACTGCTTCCTTAAACATTAGTCCAACACTATCTTTTTTTAGCAAAATACTTTAGTGATTTTCTTTCCAAAGAAAAGCATCGGTT‑TGT‑AAAAAAGATATGCATTAAATAAAA‑AACGACCAGCTTGCa                                                                                                             <  2:1981313/140‑1 (MQ=255)

                              aaCCAATGGCTTCCATTGCTTCATGAAAAATTAGTACAACAGCATATTGATTTACGAAAAGACTTTACTGATATTCTTTCCAAAGAAAAGCATCCGGT‑TGT‑AAAAAATAAATGCAGTAAATAAAA‑CACGACCAGCTTGCa                                                                                                             <  2:3141564/140‑1 (MQ=255)

                                 cAATGGCTTGCAATGCTTCCTGAAACATTAGTCCAACAGCATCTTGATTTAGCAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCACCCGGT‑TTA‑AAAAAAGAAATGCAGTAAATAAAA‑CACGACCAGCTTGCagag                                                                                                          <  2:1856349/140‑1 (MQ=255)

                                  aaTGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGT‑TGT‑AAAAAAGAAATGCAGTAAATAAAA‑AACGACCAGCTTGCagaga                                                                                                         >  2:787210/1‑140 (MQ=255)

                                   aTGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGT‑TGT‑AAAAAAGAAATGCAGTAAATAAAA‑AACTACCAGCTTGCagagag                                                                                                        >  2:3788794/1‑140 (MQ=255)

                                   aTGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGT‑TGT‑AAAAAAGAAATGCAGTAAATAAAA‑AACGACCAGCTTGCagagag                                                                                                        >  2:766019/1‑140 (MQ=255)

                                   aTGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGT‑TGT‑AAAAAAGAAATGCAGTAAATAAAA‑AACGACCAGCTTGCagagag                                                                                                        >  1:5444185/1‑140 (MQ=255)

                                   aTGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGT‑TGT‑AAAAAAGAAATGCAGTAAATAAAA‑AACGACCAGCTTGCagagag                                                                                                        >  1:4577728/1‑140 (MQ=255)

                                   aTGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGT‑TGT‑AAAAAAGAAATGCAGTAAATAAAA‑AACGACCAGCTTGCagagag                                                                                                        >  2:6581326/1‑140 (MQ=255)

                                   aTGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGT‑TGT‑AAAAAAGAAATGCAGTAAATAAAA‑AACGACCAGCTTGCagagag                                                                                                        >  2:3696455/1‑140 (MQ=255)

                                   aTGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGT‑TGT‑AAAAAAGAAATGCAGTAAATAAAA‑AACGACCAGCTTGAagagag                                                                                                        >  1:7075722/1‑140 (MQ=255)

                                    tGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGT‑TGT‑AAAAAAGATATGCAGTAAATAAAA‑AACGACCAGCTTGCAGAGAGc                                                                                                       >  1:5318616/1‑140 (MQ=255)

                                    tGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGT‑TGT‑AAAAAAGAAATGCAGTAAATAAAA‑AACTACCAGCTTGCAGAGAGc                                                                                                       >  2:5388071/1‑140 (MQ=255)

                                    tGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGT‑TGT‑AAAAAAGAAATGCAGTAAATAAAA‑AACGACCAGCTTGCAGAGAGc                                                                                                       >  2:2081426/1‑140 (MQ=255)

                                    tGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGT‑TGT‑AAAAAAGAAATGCAGTAAATAAAA‑AACGACCAGCTTGCAGAGAGc                                                                                                       >  2:3476303/1‑140 (MQ=255)

                                    tGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGT‑TGT‑AAAAAAGAAATGCAGTAAATAAAA‑AACGACCAGCTTGCAGAGAGc                                                                                                       >  2:3964834/1‑140 (MQ=255)

                                    tGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGT‑TGT‑AAAAAAGAAATGCAGTAAATAAAA‑AACGACCAGCTTGCAGAGAGc                                                                                                       >  1:1568361/1‑140 (MQ=255)

                                           cAATCCTCCCTCAACAATTACTTCCACCCCATCTTGATTGAGGAAAAGACTTGAGTGATAGTCTGTCGAAAGAAACGCAGCCGGT‑GTT‑AAAAAACAAAGGCAGTAAATAAAA‑ACCGACCAGCTTGCAGAGAGCAATCGtt                                                                                                <  2:5164554/140‑1 (MQ=255)

                                           aattGCTACCTTAAACATTTTTTCAACACCATCTTGATTAAGTAAAAGACTTTACTTATATTCTTTCCAAAGAAACCCATCCGGT‑TGT‑AAAAAACAAAGGCATTAAATAAAA‑AACGACCAGCTTGCAGAGAGCAATCGtt                                                                                                <  2:6780161/137‑1 (MQ=255)

                                           aaaTGCTTCTTTAACCATTAGTTAATCATCTTCTTTTTTTAGTAAATTATTTTTCTTTTTTTCTTTCTAAATAAAAGCTTCGGTT‑TTT‑AAAAATTAATTGCAGTATTTAAAA‑AAAGAACAGATTGCAGAGAGCAATCGtt                                                                                                <  2:5494543/139‑1 (MQ=255)

                                                cTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGT‑TGT‑AAAAAAGAAATGCAGTAAATAAAA‑AACGACCAGCTTGCAGAGAGCAATCGTTCTCTg                                                                                           >  2:3252412/1‑140 (MQ=255)

                                                cTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGT‑TGT‑AAAAAAGAAATGCAGTAAATAAAA‑AACGACCAGCTTGCAGAGAGCAATCGTTCTCTg                                                                                           >  1:2324434/1‑140 (MQ=255)

                                                 ttCCTTAAACTTTATTACAAAATCATCTTGATTTATTAAAAGACTTTACTTATATTATTTCGAAATAAAAGATTCCGGT‑TTT‑AAAAAACAAAGGCATTAAATAAAA‑AACGACCAGCTTGCAGAGAGCAATCGTTCTCTGc                                                                                          <  2:4576525/140‑1 (MQ=255)

                                                  tCCTTAAACATTATTTCAACCGCATTTTGATTTCGTAAAAGACTTTACTGATATTGTTTCCAAAGAAAAGCATCCGTT‑TTT‑AAAAAAGAAAGGCATTAAATAAAA‑AACGACCAGCTTGCAGAGAGCAATCGTTCTCTGCa                                                                                         <  2:1057345/140‑1 (MQ=255)

                                                  tCCTGAAAAATTAGTTCAACACAATCTTTTTTTAGGAAAAGAATTTACTGATATTCTTTCGAAAGAAAAGCATCCGGT‑TTT‑AAAAAAGAAATGCATTAAATAAAA‑AACGACCAGCTTGCAGAGAGCAATCGTTCTCTGCa                                                                                         <  2:7601678/140‑1 (MQ=255)

                                                  accTAAAACATTATTACAACAGCTTCTTTATTTAGCAAAACACTTTACTTATTTTCTTTCGAAATAAAAGCATCCGTT‑TGT‑AAAAAACAAATGACGTAAATAAAA‑AACGACCAGCTTGCAGAGAGCAATCGTTCTCTGCa                                                                                         <  2:3582596/139‑1 (MQ=255)

                                                   cattaaaCATTAGTCAAAAAGCATGTTTATTACTCAAAACAATTTACTTTTATTGTTTCGAAATAAAAGAATCGGGT‑TGA‑AAAAAACAAATGCAGTAAATAAAA‑AACGACCAGCTTGCAGAGAGCAATCGTTCTCTGCaa                                                                                        <  2:5072188/136‑1 (MQ=255)

                                                    caaaaaCATTAGTACAACACAATGTTTATTTATCAAAAGATTTTACTCATATTATTACCAAATAAACCCAGCCGGT‑TTA‑AAAAAACAAATCCACTAAATAAAA‑CACGACCAGCTTGCAGAGAGCAATCGTTCTCTGCAAg                                                                                       <  1:4508256/137‑1 (MQ=255)

                                                     acaaaaATTAGTACAACACAATCTTTTTTTAGCAAAATAGTTTATTCATCTTCTTTCCAAACAAACGCAGCCGTT‑TG‑‑AAAAAAAAAACGCCATAAAAAAAACCACGACCAGCTTGCAGAGAGCAATCGTTCTCTGCAAGc                                                                                      <  1:4997971/138‑1 (MQ=255)

                                                          cATTAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGT‑TGT‑AAAAAAGAAATGCAGTAAATAAAA‑AACGACCAGCTTGCAGAGAGCAATCGTTCTCTGCAAGCGTTta                                                                                 >  1:1054316/1‑140 (MQ=255)

                                                            ttcgtacAACAGAACCTTGTTTTAGAAAAACAATTTACTCATATTCTTTCCAAAGAAACGCATCGGTT‑TTT‑AAACAAGAACTGCACAAAATAAAC‑CACGACCAGCTTGCAGAGAGCAATCGTTCTCTGCAAGCCTTtata                                                                               <  1:961841/134‑1 (MQ=255)

                                                            ttATTTCAACAGATTGTTTTTTTATTAAAATACTTTACTATTTTTCTTTCCAAATAAAAGCATCGGTT‑TGA‑AAAAAATAAAGCCAGAAAAAAAAA‑CACGACCAGCTTGCAGAGAGCAATCGTTCTCTGCAAGCCTTtata                                                                               <  1:6243766/140‑1 (MQ=255)

                                                            ttAGTACAACAGCATCTTTTTTTAGTAAAATACTTTACTTATATTATTTATAAATAAAAGCATCGGTT‑TTT‑AAAAAAGAAATCCAGTAAAAAAAA‑AACGACCAGCTTGCAGAGAGCAATCGTTCTCTGCAAGCCTTtata                                                                               <  1:7757509/140‑1 (MQ=255)

                                                             tAGTTCAACAGCATGTTGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGT‑TGT‑AAAAATGAAATGCAGTAAATAAAA‑AACGACCAGCTTGCAGAGAGCAATCGTGCTCTGCAAGCCTTtatat                                                                              >  1:25233/1‑139 (MQ=255)

                                                             tAGTTCAACAGCATCTTGATTTATGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGT‑TGT‑AAAAAAGAAATGCAGTAAATAAAA‑AACGACCAGCTTGCAGAGAGCAATCGTTCTCTGCAAGCCTTTATaa                                                                              >  2:900331/1‑140 (MQ=255)

                                                             tAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGT‑TGT‑AAAAAAGAAATGCAGTAAATAAAA‑AACGACCAGCTTGCAGAGAGCAATCGTTCTCTGCAAGCCTTTATaa                                                                              >  2:6889082/1‑140 (MQ=255)

                                                               gtacAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGT‑TGT‑AAAAAAGAAATGCAGTAAATAAAA‑AACGACCAGCTTGCAGAGAGCAATCGTTCTCTGCAAGCCTTTATAAgg                                                                            <  2:1608411/137‑1 (MQ=255)

                                                                          tCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGT‑TGT‑AAAAAAGAAATGCAGTAAATAAAA‑AACGACCAGCTTGCAGAGAGCAATCGTTCTCTGCAAGCCTTTATAAGGAGATGTACCAg                                                                 >  2:5444861/1‑140 (MQ=255)

                                                                          tCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGT‑TGT‑AAAAAAGAAATGCAGTAAATAAAA‑AACGACCAGCTTGCAGAGAGCAATCGTTCTCTGCAAGCCTTTATAAGGAGATGTACCAg                                                                 >  1:3178820/1‑140 (MQ=255)

                                                                          tCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGT‑TGT‑AAAAAAGAAATGCAGTAAATAAAA‑AACGACCAGCTTGCAGAGAGAAATCGTTCTCTGCAATCCTTTATAAGGAGATGTACCAg                                                                 >  1:1874575/1‑140 (MQ=255)

                                                                             ttaattATGAAAAGAGTTTCCTGATTTTCTTTCTAATTAAAAGCAACCGGT‑TTT‑AAAAAATATATGCAGTAAAAAAAA‑AACGACCAGCTTGCAGAGAGCAATCGTTCTCTGCAAGCCTTTATAAGGAGATGTACCAGTGa                                                              <  2:4537107/136‑1 (MQ=255)

                                                                               aTTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGT‑TGT‑AAAAAAGAAATGCAGTAAATAAAA‑AACGACCAGCTTGCAGAGAGCAATCGTTCTCTGCAAGCCTTTATAAGGAGATGTACCAGTGaaa                                                            >  2:7048281/1‑140 (MQ=255)

                                                                                  tAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGT‑TGT‑AAAAAAGAAATGCAGTAAATAAAA‑AACGACCAGCTTGCAGAGAGCAATCGTTCTCTGCAAGCCTTTATAAGGAGATGTACCAGTGAAACAt                                                         >  2:4633743/1‑140 (MQ=255)

                                                                                  tAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGT‑TGT‑AAAAAAGAAATGCAGTAAATAAAA‑AACGACCAGCTTGCAGAGAGCAATCGTTCTCTGCAAGCCTTTATAAGGAGATGTACCAGTGAAACAt                                                         >  2:3514327/1‑140 (MQ=255)

                                                                                  tAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGT‑TGT‑AAAAAAGAAATGCAGTAAATAAAA‑AACGACCAGCTTGCAGAGAGCAATCGTTCTCTGCAAGCCTTTATAAGGAGATGTACCAGTGAAACAt                                                         >  1:7307054/1‑140 (MQ=255)

                                                                                  tAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGT‑TGT‑AAAAAAGAAATGCAGTAAATAAAA‑AACGACCAGCTTGCAGAGAGCAATCGTTCTCTGCAAGCCTTTATAAGGAGATGTACCAGTGAAACAt                                                         >  1:4246906/1‑140 (MQ=255)

                                                                                  tAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGT‑TGT‑AAAAAAGAAATGCAGTAAATAAAA‑AACGACCAGCTTGCAGAGAGCAATCGTTCTCTGCAAGCCTTTATAAGGAGATGTACCAGTGAAACAt                                                         >  1:6867596/1‑140 (MQ=255)

                                                                                    ggAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGT‑TGT‑AAAAAAGAAATGCAGTAAATAAAA‑AACGACCAGCTTGCAGAGAGCAATCGTTCTCTGCAAGCCTTTATAAGGAGATGTACCAGTGAAACATCa                                                       >  2:119379/1‑140 (MQ=255)

                                                                                               tACTGATATTCTTTCGAAAGAAAAGCATCCGGT‑TGT‑AAAAAAGAAATGCAGTAAATAAAA‑AACGACCAGCTTGCAGAGAGCAATCGTTCTCTGCAAGCCTTTATAAGGAGATGTACCAGTGAAACATCAAATTTCTTCaa                                            >  1:5734228/1‑140 (MQ=255)

                                                                                                     ttttatttCTAAATAAAAGCAACCGTT‑TTA‑AAAAAACAAATCAATTAAATAAAA‑CACGACCAGCTTGCAGAGAGCAATCGTTCTCTGCAAGCCTTTATAAGGAGATGTACCAGTGAAACATCAAATTTCTTCAAAACGCt                                      <  1:2062475/135‑1 (MQ=255)

                                                                                                     aatTCTTTCCAAACAAAAG‑ATCCCGT‑TTTAAAAAAACAAATCCATAAAATAAAA‑AACGACCAGCTTGCAGAGAGCAATCGTTCTCTGCAAGCCTTTATAAGGAGATGTACCAGTGAAACATCAAATTTCTTCAAAACGCt                                      <  1:6582843/139‑1 (MQ=255)

                                                                                                       ttCTTTCGAAAGAAAAGCATCCGGT‑TGT‑AAAAAAGAAATGCAGTAAATAAAA‑AACGACCAGCTTGCAGAGAGCAATCGTTCTCTGCAAGCCTTTATAAGGAGATGTACCAGTGAAACATCAAATTTCTTCAAAACGCTTa                                    >  2:591479/1‑140 (MQ=255)

                                                                                                          tttCTAAACAAACGCATCGGGT‑TGT‑AAAAAATAAATGCAGTAAATAAAC‑CACGACCAGCTTGCAGAGAGCAATCGTTCTCTGCAAGCCTTTATAAGGAGATGTACCAGTGAAACATCAAATTTCTTCAAAACGCTTATTg                                 <  1:3763715/140‑1 (MQ=255)

                                                                                                          tttCCAAACAAAAGCATCCGTT‑TTT‑AAAAAACAAATGCAGTAAAAAAAA‑CACGACCAGCTTGCAGAGAGCAATCGTTCTCTGCAAGCCTTTATAAGGAGATGTACCAGTGAAACATCAAATTTCTTCAAAACGCTTATTg                                 <  1:3788794/140‑1 (MQ=255)

                                                                                                           ttCGAAAGAAAAGCATCCGGT‑TGT‑AAAAAAGAAATGCAGTAAATAAAA‑AACGACCAGCTTGCAGAGAGCAATCGTTCTCTGCAAGCCTTTATAAGGAGATGTACCAGTGAAACATAAAATTTCTTCAAAACGCTTATTGc                                >  1:7614210/1‑140 (MQ=255)

                                                                                                            tcctaagaaaTGCATGCTTT‑TTT‑AAAAATTAAATGAAGTAAATAAAA‑AAAGACCAGCTTTAAGAGAGCAATAGTTCTCTGAAAGACTTTATAAGGAGATGTACCAGTGAAACATCAAATTTCTTCAAAACGCTTATTGCg                               <  2:5717028/136‑1 (MQ=255)

                                                                                                            tCGAAAGAAAAGCATCCGGT‑TGT‑AAAAAAGAAATGCAGTAAATAAAA‑AACGACCAGCTTGCAGAGAGCAATCGTTCTCTGCAAGCCTTTATAAGGAGATGTACCAGTGAAACATCAAATTTCTTCAAAACGCTTATTGCg                               >  2:2440667/1‑140 (MQ=255)

                                                                                                            tCGAAAGAAAAGCATCCGGT‑TGT‑AAAAAAGAAATGCAGTAAATAAAA‑AACGACCAGCTTGCAGAGAGCAATCGTTCTCTGCAAGCCTTTATAAGGAGATGTACCAGTGAAACATCAAATTTCTTCAAAACGCTTATTGCg                               >  2:6393235/1‑140 (MQ=255)

                                                                                                              gaaagaaaCGCACCCGGT‑TTT‑AAAAAATAAATGATTTAAATAAAA‑CACGACCAGCTTGCAGAGAGCAATCGTTCTCTGCAAGCCTTTATAAGGAGATGTACCAGTGAAACATCAAATTTCTTCAAAACGCTTATTGCGGc                             <  2:4287898/140‑1 (MQ=255)

                                                                                                              caaagaaaCGCTTCCGGT‑TGT‑AAAAAAGAAAGGCAGTAAATAAAA‑AACGACCAGCTTGCAGAGAGCACTCGTTCTCTGCAAGCCTTTATAAGGAGATGTACCAGTGAAACATCAAATTTCTTCAAAACGCTTATTGCGGc                             <  2:2248074/139‑1 (MQ=255)

                                                                                                              caaagaaaAGCATCCGGT‑TGT‑AAAAAAGAAATGCAGTAAATAAAA‑AACGACCAGCTTGCAGAGAGCAATCGTTCTCTGCAAGCCTTTATAAGGAGATGTACCAGTGAAACATCAAATTTCTTCAAAACGCTTATTGCGGc                             <  2:4062882/139‑1 (MQ=255)

                                                                                                               ccagaaaAGCATCCGGG‑TCT‑AAAAAAGAAATGCAGTAAATAAAA‑AACGACCAGCTTGCAGAGAGCAATCGTTCTCTGCAAGCCTTGATACGGAGATGTACCAGTGAAACATCACATTTCGTCAAAACGCTTATTGCGGct                            <  2:4332100/138‑1 (MQ=255)

                                                                                                               aactaaaaGCATCCGGT‑TGT‑AAAAAAGAAATGCAGTAAATAAAA‑CACGACCAGCTTGCAGAGAGCAATCGTTCTCTGCAAGCCTTTATCAGGAGATGTCCCAGTGAAACATCAAATTTCTTCAAAACGCTTATTGCGGct                            <  2:6173256/136‑1 (MQ=255)

                                                                                                               aaataaaAGCA‑CCGGTGTGT‑AAAAAACAAATGCAGTAAATAAAA‑CACGACCAGCTTGCAGAGAGCAATCGTTCTCTGCAAGCCTTTATAAGGAGATGTACCAGTGAAACATCAAATTTCTTCAAAACGCTTATTGCGGct                            <  1:854915/140‑1 (MQ=255)

                                                                                                               aaacaaaAGCCTGCGGT‑TTT‑AAAAAACAAATGCAGTAAATAAAA‑AACGACCAGCTTGCAGAGAGCAATCGTTCTCTGCAAGCCTTGATAAGGAGATGTACCAGTGAAACATCAAATTTCTTCAAAACGCTTATTGCGGct                            <  1:4303956/140‑1 (MQ=255)

                                                                                                                    aaaGCATCCGGT‑TGT‑AAAAAAGAAATGCAGTAAATAAAA‑AACGACCAGCTTGCAGAGAGCAATCGTTCTCTGCAAGCCTTTATAAGGAGATGTACCAGTGAAACATCAAATTTCTTCAAAACGCTTATTGCGGCTCTTta                       <  2:7548194/140‑1 (MQ=255)

                                                                                                                     aaGCATCCGGT‑TTA‑AAAAAACATAGCCAGTAAAAAAAA‑CACGACCAGCTTGCAGAGAGCAATCGTTCTCTGCAAGCCTTTATAAGGAGATGTACCAGTGAAACATCAAATTTCTTCAAAACGCTTATTGCGGCTCTTtat                      <  1:2093647/140‑1 (MQ=255)

                                                                                                                      accacccGGT‑TTA‑AAAAAAAAAATCAATAAAATAAAA‑AACGACCAGCTTGCAGAGAGCAATCGTTCTCTGCAAGCCTTTATAAGGAGATGTACCAGTGAAACATCAAATTTCTTCAAAACGCTTATTGCGGCTCTTtata                     <  2:6623643/135‑1 (MQ=255)

                                                                                                                      accATCCGGT‑TTA‑AAAAAATAAAGCCAGTAAATAAAA‑AACGACCAGCTTGCAGAGAGCAATCGTTCTCTGCAAGCCTTTATAAGGAGATGTACCAGTGAAACATCAAATTTCTTCAAAACGCTTATTGCGGCTCTTtata                     <  1:362743/138‑1 (MQ=255)

                                                                                                                      aGCATCCGTT‑TGT‑AAAAAAGAACTCCAGAAAATAAAA‑AACGACCAGCTTGCAGAGAGCAATCGTTCTCTGCAAGCCTTTATAAGGAGATGTACCAGTGAAACATCAAATTTCTTCAAAACGCTTATTGCGGCTCTTtata                     <  2:5420725/140‑1 (MQ=255)

                                                                                                                      aGCATCCGGT‑TTA‑AAAAAACAAATGCAGAAAATAAAA‑AACGACCAGCTTGCAGAGAGCAATCGTTCTCTGCAAGCCTTTATAAGGAGATGTACCAGTGAAACATCAAATTTCTTCAAAACGCTTATTGCGGCTCTTtata                     <  1:1791489/140‑1 (MQ=255)

                                                                                                                         aTCCGGT‑TTT‑AAAAAACAAATGCAGTAAATAAAA‑AACGACCAGCTTGCAGAGAGCAATCGTTCTCTGCAAGCCTTTATAAGGAGATGTACCAGTGAAACATCAAATTTCTTCAAAACGCTTATTGCGGCTCTTTATAAAg                  <  2:586770/140‑1 (MQ=255)

                                                                                                                                   t‑AAAAAAGAAATGCAGTAAATAAAA‑AACGACCAGCTTGCAGAGAGCAATCGTTCTCTGCAAGCCTTTATAAGGAGATGTACCAGTGAAACATCAAATTTCTTCAAAACGCTTATTGCGGCTCTTTATAAAGTACTCGCTg         >  1:3893733/1‑140 (MQ=255)

                                                                                                                                      aaaaaGAAATGCAGTAAATAAAA‑AACGACCAGCTTGCAGAGAGCAATCGTTCTCTGCAAGCCTTTATAAGGAGATGTACCAGTGAAACATCAAATTTCTTCAAAACGCTTATTGCGGCTCTTTATAAAGTACTCGCTGGc       >  2:7350878/1‑140 (MQ=255)

                                                                                                                                      aaaaaGAAATGAAGTAAATAAAA‑AACGACCAGCTTGCAGAGAGCAATCGTTCTCTGCAAGCCTTTATAAGGAGATGTACCAGTGAAACATCAAATTTCTTCAAAACGCTTATTGCGGCTCTTTATAAAGTACTCGCTGGc       <  2:1482313/140‑1 (MQ=255)

                                                                                                                                         aaGAATTCCAGTAAATAAAA‑CACGACCAGCTTGCAGAGAGCAATCGTTCTCTGCAAGCCTTTATAAGGAGATGTACCAGTGAAACATCAAATTTCTTCAAAACGCTTATTGCGGCTCTTTATAAAGTACTCGCTGGCcgc    <  2:7155647/140‑1 (MQ=255)

                                                                                                                                           gAAATCAAGAAAAAAAAC‑AACGACCAGCTTGCAGAGAGCAATCGTTCTCTGCAAGCCATTAGAAGGAGAGGTACCAGTGAACCATCACATTTCTTCAAAACGCTTATTGCGGCTCTTTATAAAGTACTCGCTGGCCGCGa  <  1:287444/140‑1 (MQ=255)

AAAGACGATCATTATGCCGATGTATCTTTAAACCAATGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGT‑TGT‑AAAAAAGAAATGCAGTAAATAAAA‑AACGACCAGCTTGCAGAGAGCAATCGTTCTCTGCAAGCCTTTATAAGGAGATGTACCAGTGAAACATCAAATTTCTTCAAAACGCTTATTGCGGCTCTTTATAAAGTACTCGCTGGCCGCGA  >  NZ_CP034943/3219668‑3219944

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`