BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	3,219,734	C→A	7.1%	Q358K (CAA→AAA)	oxdC →	oxalate decarboxylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	3,219,734	0	C	A	7.1%	513.5 / 3.2	202	Q358K (CAA→AAA)	oxdC	oxalate decarboxylase
Reads supporting (aligned to +/- strand): ref base C (104/75); new base A (2/21); total (106/96)
Fisher's exact test for biased strand distribution p-value = 6.95e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 203 total aligned reads displayed.

TGTTCCATTTGCAATGGGTCATTACGTTGAAAACATCGGGGACGAACCGCTTGTCTTTTTAGAAATCTTTAAAGACGATCATTATGCCGATGTATCTTTAAACCAATGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGTTG‑T‑AAAAAAGAAATGCAGTAAATAAAA‑AACGACCAGCTTGCAGAGAGCAATCGTTCTCTGCAAGCCTTTATAAGG  >  NZ_CP034943/3219598‑3219870

tGTTCCATTTGCAATGGGTCATTACGTTGAAAACATCGGGGACGAACCGCTTGTCTTTTTAGAAATCTTTAAAGACGATCATTATGCCGATGTATCTTTAAACCAATGGCTTGCAATGCTTCCTGAAACATTAGTTCAAc                                                                                                                                          <  2:1185891/140‑1 (MQ=255)

tGTTCCATTTGCAATGGGTCATTACGTTGAAAACATCGGGGACGAACCGCTTGTCTTTTTAGAAATCTTTAAAGACGATCATTATGCCGATGTATCTTTAAACCAATGGCTTGCAATGCTTCCTGAAACATTAGTTCAAc                                                                                                                                          >  1:1472622/1‑140 (MQ=255)

 gTTCCATTTGCAATGGGTCATTACGTTGAAAACATCGGGGACGAACCGCTTGTCTTTTTAGAAATCTTTAAAGACGATCATTATGCCGATGTATCTTTAAACCAATGGCTTGCAATGCTTCCTGAAACATTAGTTCAACa                                                                                                                                         >  1:1176985/1‑140 (MQ=255)

  ttCCATTTGCAATGGGTCATTACGTTGAAAACATCGGGGACGAACCGCTTGTCTTTTTAGAAATCTTTAAAGACGATCATTATGCCGATGTATCTTTAAACCACTGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAg                                                                                                                                        >  2:1249443/1‑140 (MQ=255)

   tCCATTTGCAATGGGTCATTACGTTGAAAACATCGGGGACGAACCGCTTGTCTTTTTAGAAATCTTTAAAGACGATCATTATGCCGATGTATCTTTAAACCAATGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGc                                                                                                                                       >  1:2445102/1‑140 (MQ=255)

     cATTTGCAATGGGTCATTACGTTGAAAACATCGGGGACGAACCGCTTGTCTTTTTAGAAATCTTTAAAGACGATCATTATGCCGATGTATCTTTAAACCAATGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCAt                                                                                                                                     >  1:3113472/1‑140 (MQ=255)

          gCAATGGGTCATTACGTTGAAAACATCGGGGACGAACCGCTTGTCTTTTTAGAAATCTTTAAAGACGATCATTATGCCGATGTATCTTTAAACCAATGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGa                                                                                                                                >  1:1261521/1‑140 (MQ=255)

          gCAATGGGTCATTACGTTGAAAACATCGGGGACGAACCGCTTGTCTTTTTAGAAATCTTTAAAGACGATCATTATGCCGATGTATCTTTAAACCAATGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGa                                                                                                                                >  1:231745/1‑140 (MQ=255)

          gCAATGGGTCATTACGTTGAAAACATCGGGGACGAACCGCTTGTCTTTTTAGAAATCTTTAAAGAAGATCATTATGCCGATGTATCTTTAAACCAATGGCTTGCAATGCTTCCTGAAACATTAGTTCAAAAGCATCTTGa                                                                                                                                >  1:2702012/1‑140 (MQ=255)

            aaTGGGTCATTACGTTGAAAACATCGGGGACGAACCGCTTGTCATTTGAGAAATCTTTAAAGACGATCATTATGCCGATGTATCTTTAAACCAATGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGAtt                                                                                                                              <  2:2849513/140‑1 (MQ=255)

                 gTCATTACGTTGAAAACATCGGGGACGAACCGCTTGTCTTTTTAGAAATCTTTAAAGACGATCATTATGCCGATGTATCTTTAAACCAATGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGa                                                                                                                         <  1:820684/140‑1 (MQ=255)

                 gTCATTACGTTGAAAACATCGGGGACGAACCGCTTGTCTTTTTAGAAATCTTTAAAGACGATCATTATGCCGATGTATCTTTAAACCAATGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGa                                                                                                                         <  2:3578962/140‑1 (MQ=255)

                 gTCATTACGTTGAAAACATCGGGGACGAACCGCTTGTCTTTTTAGAAATCTTTAAAGACGATCATTATGCCGATGTATCTTTAAACCAATGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGa                                                                                                                         <  1:3741512/140‑1 (MQ=255)

                 gTCATTACGTTGAAAACATCGGGGACGAACCGCTTGTCTTTTTAGAAATCTTTAAAGACGATCATTATGCCGATGTATCTTTAAACCAATGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGa                                                                                                                         <  1:568655/140‑1 (MQ=255)

                  tCATTAATTTGAAAACATAGGTGACGAACCTCTTTTCTTTTTAGAAATCTTTAAAGACGATCATTATGCCGATTTATCTTTAAACCAATGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTTATTTAGGaa                                                                                                                        <  1:3583181/140‑1 (MQ=255)

                      tACGTTGAAAACATCGGGGACGAACCGCTTGTCTTTTTAGAAATCTTTAAAGACGATCATTATGCCGATGTATCTTTAAACCAATGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGa                                                                                                                    <  1:279539/140‑1 (MQ=255)

                      tACGTTGAAAACATCGGGGACGAACCGCTTGTCTTTTTAGAAATCTTTAAAGACGATCATTATGCCGATGTATCTTTAAACCAATGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGa                                                                                                                    >  1:2965682/1‑140 (MQ=255)

                         gTTGAAAACATCGGGGACGAACCGCTTGTCTTTTTAGAAATCTTTAAAGACGATCATTATGCCGATGTATCTTTAAACCAATGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACtt                                                                                                                 <  1:3656959/140‑1 (MQ=255)

                         gTTGAAAACATCGGGGACGAACCGCTTGTCTTTTTAGAAATCTTTAAAGACGATCATTATGCCGATGTATCTTTAAACCAATGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACtt                                                                                                                 <  2:1472622/140‑1 (MQ=255)

                          ttGAAAACATCGGGGACGAACCGCTTGTCTTTTTAGAAATCTTTAAAGACGATCATTATGCCGATGTATCTTTAAACCAATGGCTTGCAATGCTTCCTGATACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACttt                                                                                                                >  1:2675491/1‑140 (MQ=255)

                          ttGAAAACATCGGGGACGAACCGCTTGTCTTTTTAGAAATCTTTAAAGACGATCATTATGCCGATGTATCTTTAAACCAATGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACttt                                                                                                                >  1:3238501/1‑140 (MQ=255)

                          ttGAAAACATCGGGGACGAACCGCTTGTCTTTTTAGAAATCTTTAAAGACGATCATTATGCCGATGTATCTTTAAACCAATGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACttt                                                                                                                >  1:1381171/1‑140 (MQ=255)

                          ttGAAAACATCGGGGACGAACCGCTTGTCTTTTTAGAAATCTTTAAAGACGATCATTATGCCGATGTATCTTTAAACCAATGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACttt                                                                                                                >  2:2745161/1‑140 (MQ=255)

                          ttGAAAACATCGGGGACGAACCGCTTGTCTTTTTAGAAATCTTTAAAGACGATCATTATGCCGATGTATCTTTAAACCAATGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACttt                                                                                                                >  2:3085491/1‑140 (MQ=255)

                          ttGAAAACATCGGGGACGAACCGCTTGTCTTTTTAGAAATCTTTAAAGACGATCATTATGCCGATGTATCTTTAAACCAATGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACttt                                                                                                                >  2:1365757/1‑140 (MQ=255)

                           tGAAAACATCGGGGACGAACCGCTTGTCTTTTTAGAAATCTTTAAAGACGATCATTATGCCGATGTATCTTTAAACCAATGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTa                                                                                                               >  1:1822503/1‑140 (MQ=255)

                           tGAAAACATCGGGGACGAACCGCTTGTCTTTTTAGAAATCTTTAAAGACGATCATTATGCCGATGTATCTTTAAACCAATGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACGTTa                                                                                                               >  2:3336277/1‑140 (MQ=255)

                           tGAAAACATCGGGGACGAACCGCTTGTCTTTTTAGAAATCTTTAAAGACGATCATTATGCCGATGTATCTTTAAACCAATGGCTTGCAATGCTTCCTGAAACATTAGTTAAACAGCATCTTGATTTAGGAAAAGACTTTa                                                                                                               >  1:409285/1‑140 (MQ=255)

                           tGAAAACATCGGGGACGAACCGCTTGTCTTTTTAGAAATCTTTAAAGACGATCATTATGCCGATGTATCTTTAAAACAATGGCTTGCAATGCTTCCTGAAACATTAGTTCAAGATCATCTTGATTTAGGAAAAGACTTTa                                                                                                               >  1:2580898/1‑140 (MQ=255)

                           tGAAAACATCGGGGACGAACCGCTTGTCTGTTTAGAAATCTTTAAAGACGATCATTCTGCCGATGTATCGTTAAACCAATGGCTTGCAATGCTTCCTGACACATGAGTTCAAGAGCATCTGGAGTTAGGACAAGACTTTa                                                                                                               >  1:2841921/1‑140 (MQ=255)

                             aaaaCATCGGGGACGAACCGCTTGTCTTTTTAGAAATCTTTAAAGACGATCATTATGCCGATGTATCTTTAAACCAATGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTGAGGAAAAGACTTTACt                                                                                                             >  1:684191/1‑140 (MQ=255)

                                  aTCGGGGACGAACCGCTTGTCTTTTTAGAAATCTTTAAAGACGATCATTATGCCGATGTATCTTTAAACCAATGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGatat                                                                                                        >  2:2473531/1‑140 (MQ=255)

                                  aTCGGGGACGAACCGCTTGTCTTTTTAGAAATCTTTAAAGACGATCATTATGCCGATGTATCTTTAAACCAATGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGatat                                                                                                        >  2:2099190/1‑140 (MQ=255)

                                    cGGGGACGAACCGCTTGTCTTTTTAGAAATCTTTAAAGACGATCCTTATGCCGATGTATCTTTAAACCAATGGGTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTAATGATATTc                                                                                                      >  2:1507765/1‑140 (MQ=255)

                                       ggACGAACCTCTTGTCTTTTTAGAAATCTTTAAAGACGATCATTATGCCGATGTATCTTTAAACCAATGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCttt                                                                                                   <  2:2217416/140‑1 (MQ=255)

                                       ggACGAACCTCTTGTCTTTTTAGAAATATTTAAAGACGATCATTATGCCGATGTATCTTTAAACCAATGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCttt                                                                                                   <  2:3260543/140‑1 (MQ=255)

                                          cGAACCGCTTGTCTTTTTAGAAATCTTTAAAGACGATCATTATGCCGATGTATCTTTAAACCAATGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCga                                                                                                <  2:460743/140‑1 (MQ=255)

                                            aaCCGCTTGTCTTTTTAGAAATCTTTAAAGACGATCATTATGCGGATGTATCTTTAAACCAATTGCTTGCACTGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCgaaa                                                                                              >  2:2688288/1‑140 (MQ=255)

                                            aaCCGCTTGTCTTTTTAGAAATCTTTAAAGACGATCATTATGCCGATGTATCTTTAAACCAATGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCgaaa                                                                                              >  2:110613/1‑140 (MQ=255)

                                            aaCCGCTTGTCTTTTTAGAAATCTTTAAAGACGATCATTATGCCGATGTATCTTTAAACCAATGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCgaaa                                                                                              >  2:3621711/1‑140 (MQ=255)

                                                gCTTGTCTTTTTAGAAATCTTTAAAGACGATCATTATGCCGATGTATCTTTAAACCAATGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCgaaagaaa                                                                                          >  1:3028297/1‑140 (MQ=255)

                                                 cTTGTCTTTTTAGAAATCTTTAAAGACGATCATTATGCCGATGTATCTTTAAACCAATGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGaaaa                                                                                         >  1:3382654/1‑140 (MQ=255)

                                                         tttATAAATCTTTAAAGACGATCATTATGCCGATGTATCTTTAAACCAATGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCgg                                                                                 <  1:3360163/140‑1 (MQ=255)

                                                         tttAGAAATCTTTAAAGACGATCATTATGCCGATGTATCTTTAAACCAATGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCgg                                                                                 >  2:1669328/1‑140 (MQ=255)

                                                         tttAGAAATCTTTAAAGAAGATCATTATGCCGATGTATCTTTAAACCAATGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCgg                                                                                 >  2:162633/1‑140 (MQ=255)

                                                               aaTCTTTAAAGACGATCATTATGCCGATGTATCTTTAAACCAATGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGTTG‑T‑aa                                                                         <  1:646921/140‑1 (MQ=255)

                                                               aaTCTTTAAAGACGATCATTATGCCGATGTATCTTTAAACCAATGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGTTG‑T‑aa                                                                         <  2:716626/140‑1 (MQ=255)

                                                                    ttAAAGACGATCATTATGCCGATGTATCTTTAAACCAATGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGTTG‑T‑AAAAAAg                                                                    <  1:1669328/140‑1 (MQ=255)

                                                                    ttAAAGACGATCATTATGCCGATGTATCTTTAAACCAATGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGTTG‑T‑AAAAAAg                                                                    <  1:162633/140‑1 (MQ=255)

                                                                            gATCATTATGCCGATGTATCTTTAAACCAATGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGTTG‑T‑AAAAAAGAAATGCAg                                                            <  2:1236571/140‑1 (MQ=255)

                                                                            gATCATTATGCCGATGTATCTTTAAACCAATGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGTTG‑T‑AAAAAAGAAATGCAg                                                            <  2:1651935/140‑1 (MQ=255)

                                                                             aTCATTATGCCGATGTATCTTTAAACCAATGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAATAAAAGCATCCGGTTG‑T‑AAAAAAGAAATGCAGt                                                           >  2:2276588/1‑140 (MQ=255)

                                                                             aTCATTATGCCGATGTATCTTTAAACCAATGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGTTG‑TAAAAAAAGAAATGCAg                                                            <  2:342733/140‑1 (MQ=255)

                                                                             aTCATTATGCCGATGTATCTTTAAACCAATGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGTTG‑T‑AAAAAAGAAATGCAGt                                                           >  2:2819227/1‑140 (MQ=255)

                                                                             aTCATTATGCCGATGTATCTTTAAACCAATGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGTTG‑T‑AAAAAAGAAATGCAGt                                                           >  2:1768243/1‑140 (MQ=255)

                                                                             aTCATTATGCCGATGTATCTTTAAACCAATGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGTTG‑T‑AAAAAAGAAATGCAGt                                                           <  2:510532/140‑1 (MQ=255)

                                                                             aTCATTATGCCGATGTATCTTTAAACCAATGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGATG‑T‑AAAAAAGAAATGCAGt                                                           >  1:3506808/1‑140 (MQ=255)

                                                                                      ccGATGTATCTTTAAACCAATGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATGTTGATTTAGGAAAAGACTTTACGGATCTTCTTTCGAAAGAAAAGCATCCGGTTG‑T‑TAAAAAGAAATGCAGtaaataaaca                                                  >  2:2043975/1‑138 (MQ=255)

                                                                                      ccGATGTATCTTTAAACCAATGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGTTG‑T‑AAAAAAGAAATGCAGTACATaaaa‑a                                                 >  2:1750696/1‑140 (MQ=255)

                                                                                         aTGTATCTTTAAACCAATGGGTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGAGTTAGTAAAAGACTTTACTGATATTCTTTCGAAAGACCAGCAGCCGGTTG‑T‑ACACAAGAAATGCAGTAAATAAAA‑ACCg                                              >  2:1404332/1‑140 (MQ=255)

                                                                                         aTGTATCTTTAAACCAATGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGATCTTCTTTCGAAAGAAAATCATCCGGTTG‑T‑AAAAAAGAAATGCAGTAAATAAAA‑AAct                                              >  2:2924472/1‑139 (MQ=255)

                                                                                         aTGTATCTTTAAACCAATGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGTTG‑T‑AAAAAAGAAATGCAGTAAATAAAA‑AACg                                              >  2:2345560/1‑140 (MQ=255)

                                                                                          tttATTTTTAAACCATTGGCTTGCATTGCTTCCTGAAACATTATATAAACAGCATATTGATTTAGTAAAAGAATTTACTGATATTCTTTCGAAATAAAAGCAGCCGTTTG‑A‑AAAAAATAAATGCCTTAAATAAAA‑AACGa                                             <  2:1222114/138‑1 (MQ=255)

                                                                                          tttATTTTTAAACCAATGGCTTTCATTGCTTCCTTAAAAATTAGTTAAACAGAATCTTTATTTAGTAAAATAATTTACTGATATTCTTTCTAAACAAAAGAATCCGTTTG‑T‑AAAAAACAAATGCAGTAAATAAAA‑AACGa                                             <  2:2392706/138‑1 (MQ=255)

                                                                                          tttATTTTTAAACCAATGGCTTGCAATGTTTCATGAAAATTTATTTAAACAGAATCTTTATTTAGGAAAAGACTTTACTGATATTCTTTCTAAAGAAAAGAAGCGGGTTG‑T‑AAAAAAGAATTGCAGTAAATAAAA‑CACGa                                             <  2:2162666/138‑1 (MQ=255)

                                                                                          tttATCTTTAAACCATTGGCTTTCATTGATTCGTTAAACATTAGTTCAACAGCATTTTGATTTAGCAAAATACTTTACTTATATTCTTTCTAATTAAAAGCA‑GCGGTTGTT‑AAAAAACAAAGCCAGTAAATAAAA‑AACGa                                             <  2:2013006/138‑1 (MQ=255)

                                                                                          tttATCTTTAAAACATTGGCTTTAATTGCTTCCTGAAAATTTAGTTAAACAGAATCTTTATTTATTAAAATACTTTACTGATATTCTTTCTAAATAAAAGCATCGGGTTG‑T‑AAAAAAAAAATGCAGTAAATAAAA‑AACGa                                             <  2:2198179/138‑1 (MQ=255)

                                                                                          tGTATCTTTAAACCATTGGGTTGCAATGGTTCCGCAAAAATTAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAACAAAAGCTTCCGGTTT‑T‑AAAAAAGAAATGAAGTAAATAAAA‑AACGa                                             <  2:2500473/140‑1 (MQ=255)

                                                                                          tGTATCTTTAAACCAATGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGTTG‑T‑AAAAAAGAAATGCAGTAAATAAAA‑AACGa                                             >  2:3343696/1‑140 (MQ=255)

                                                                                          tGTATCTTTAAACCAATGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGTTG‑T‑AAAAAAGAAATGCAGTAAATAAAA‑AACGa                                             >  2:3336772/1‑140 (MQ=255)

                                                                                           gcaTCTTTAAACCAATGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGTTG‑T‑AAAAAAGAAATGCAGTAAATAAAA‑AACGAc                                            >  2:579695/3‑140 (MQ=255)

                                                                                            tATCTTTAAACCAATGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGTTG‑T‑AAAAAAGAAATGCAGTAAATAAAA‑AACGAcc                                           >  2:186626/1‑140 (MQ=255)

                                                                                                    aaCCAATGGCTTGCAATGATTCCTTAAACATTATTTAAAAAGCATCTTTTTTTAGGAAAACAATTTACTGATATTCTTTCGAAACAAAAGCATCCGGTTG‑T‑AAAAAACAAATCCAGTAAATAAAA‑AACGACCAGCTTGCa                                   <  2:2829090/140‑1 (MQ=255)

                                                                                                    aaCAAAGGGCTTCAATTGCTTCCTCAAACATTTGTTCAACAGCTACTTTATTTAGGAAAAGACTTTAATGATATTCTTTCCAAACAAAAGCATCGGGTTT‑T‑AAAAAATAAATGCAGTAAATAAAA‑AACGACCAGCTTGCa                                   <  2:669370/140‑1 (MQ=255)

                                                                                                       cAATGGATTGAAATGTTTCATTAAAAATTATTTAAACAGAATCTTTTTTTAGTAAAAGAATTTAATTATATTCTTTCTAAATAAAAGAATCCGTTTT‑T‑AAAAAAGAAATGCATTAAATAAAA‑AACGACCAGCTTGCagag                                <  2:2445102/140‑1 (MQ=255)

                                                                                                         tggggttGCATTGTTTCCTTAAAATTTATTTAAACAGCATCTTTTTTTAGGAAAACACTTTACTTTTCTTGTTTCGAACCAAAACCATCCGTTTT‑T‑AAAAAAGAAATGCTTTAAATAAAA‑AACGACCAGCTTGCagagag                              <  2:33202/135‑1 (MQ=255)

                                                                                                         aTGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGTTG‑T‑AAAAAAGAAATGCAGTAAATAAAA‑AACGACCAGCTTGAagagag                              >  2:3705577/1‑140 (MQ=255)

                                                                                                         aTGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCGTGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGTTG‑T‑AAAAAAGAAATGCAGTAAATAAAA‑AACGACCAGCTTGCagagag                              >  1:2876975/1‑140 (MQ=255)

                                                                                                         aTGGCTTGCAATGCTTCCTGAAAAATTATATCAACATCATCTAGATTTAGGAAAAGACTTTACTGATAATCTTTCGAAAGAAAAGCATCCGGTTG‑T‑AAAAAAGAAATGCAGTAAATAAAA‑AACGACCAGCTTGCagagag                              >  2:909201/1‑140 (MQ=255)

                                                                                                          tGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTTATATTCTTTCGAAAGAAAAGCATCCGGTTG‑T‑AAAAAAGTAATGCAGTACATAAAC‑AACGACCAGCTTGCAGAGAGc                             >  2:2570512/1‑140 (MQ=255)

                                                                                                            gcgtGCATTGCTCCCTGAAACATTCGTTCAACCGCATCTTTTTTTAGTAAAATACTTTACATATCTTCTTTCTAAATAAAAGCATCCGGTTG‑T‑AAAAACGAATTGCAGTAAATAAAA‑AACGACCAGCTTGCAGAGAGCaa                           <  2:3292459/137‑1 (MQ=255)

                                                                                                                 cAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGAATTTACTGATATTCTTTCGAAAGAAAAGCATCCGGTTG‑T‑AAAAAAGAAATGAAGTAAATAAAA‑AACTACCAGCTTGCAGAGAGCAATCGtt                      >  1:2066950/1‑140 (MQ=255)

                                                                                                                      cTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGATAATCTTTCGAAAGAAAAGCATCCGGTTG‑T‑AAAAAAGAAATGCAGTAAATAAAA‑AACGACCAGCATGCAGAGAGCAATCGTTCTCTg                 >  1:2608731/1‑140 (MQ=255)

                                                                                                                        tccaaaaaCATTATTACAACAGATTTTTTATTTAGAAAAAGATTTTACTTATATTCTTCCTAAACAAAACCAT‑CGGTTT‑T‑AAAAAAACAAATCAATAAAAAAAACAACGACCAGCTTGCAGAGAGCAATCGTTCTCTGCa               <  1:1365757/135‑1 (MQ=255)

                                                                                                                        tcattaaaCATTAGTTCAACAGCATCTTGTTTTAGTAAAAGACTTTACTGATTTTCTTTCGAAAGAAAAGCATCCGGTTT‑T‑AAAAAAGAAATGCAGTAAATAAAA‑AACGACCAGCTTGCAGAGAGCAATCGTTCTCTGCa               <  2:1786214/135‑1 (MQ=255)

                                                                                                                        tCCTCAAACTTTATTTCAACACAATCTTTATTTAGGAAAAGACTTTACTGATATTCTTTCGAAACAAAAGCATCCGGTTT‑T‑AAAAAAGAAATGCAGTAAATAAAA‑AACGACCAGCTTGCAGAGAGCAATCGTTCTCTGCa               <  2:3028297/140‑1 (MQ=255)

                                                                                                                         ccTCAAACATTTGTTCAACACCTTTTTTATTTTGAAAAAGACTTTACTGATATTCTTTCGAAATAAAAGATTCGGGTTT‑T‑AAAAAATAAAGCCATTAAATAAAA‑CACGACCAGCTTGCAGAGAGCAATCGTTCTCTGCaa              <  1:1797609/140‑1 (MQ=255)

                                                                                                                          cttaaaCATTTGTTAAACACAATCTTTCTTTAGTAAAAGATTTTAATGATATTCTTTCCAACGAAAAGCATCGTGTTT‑T‑AAAAAACAATTGAACTAAATAAAA‑AACGACCAGCTTGCAGAGAGCAATCGTTCTCTGCAAg             <  2:3135493/137‑1 (MQ=255)

                                                                                                                          cttaaaCATTAGTAAAACAGCAACTTGATTTAGCAAAAGACTTTACTGATATTCTTTCGAACGAAAAGCATCCGGTTG‑T‑AAAAAAGAAATGCAGTAAAAAAAA‑AACGACCAGCTTGCAGAGAGCAATCGTTCTCTGCAAg             <  2:2965682/137‑1 (MQ=255)

                                                                                                                          cacaaaCATTAGTTCAACAGAATCTTTATTTCGCAAAAGAGTTTACTTATTTTCTTTCAAAAGAAAAGCATCCGGTTG‑T‑AAAAAACAATTGAAGTAAATAAAA‑CACGACCAGCTTGCAGAGAGCAATCGTTCTCTGCAAg             <  1:2746741/137‑1 (MQ=255)

                                                                                                                          cTGAAACATTATTTCAACACAATCTTTATTTAGTAAAATACTTTAGTGATTTTCTTTCGAAAGAAAAGCAGCCGGTTT‑T‑AAAAAATAAATCCAGTAAATAAAA‑CACGACCAGCTTGCAGAGAGCAATCGTTCTCTGCAAg             <  2:389672/140‑1 (MQ=255)

                                                                                                                          cTGAAACATTAGTACAACAGAATCTTTTTTTAGCAAATGAGTTTCCTCATATTCTTTACAACAAAAACCATCGGGTTA‑T‑AAAAAAAAAATCCATTAAAAAAAA‑CACGACCAGCTTGCAGAGAGCAATCGTTCTCTGCAAg             <  1:1003750/140‑1 (MQ=255)

                                                                                                                           tcaaaCATTAGTTCAACTCCATCTTGATTTAGGAAAAGATTTTACTGATATTCTTTCCAAAGAAAAGCATCCGGTTG‑T‑AAAAAACAAATGCAGTAAATAAAA‑CACGACCAGCTTGCAGAGAGCAATCGTTCTCTGCAAGc            <  2:1730313/138‑1 (MQ=255)

                                                                                                                           tcaaaCATTAGTAAAAAATAATTTTTATTAACCAAAATATTTTACTTATATTATTTCCAAACAAAAGCATCCGTTTT‑A‑AAAAAACAAATCCAGAAAATAAAA‑CACGACCAGCTTGCAGAGAGCAATCGTTCTCTGCAAGc            <  1:3691301/138‑1 (MQ=255)

                                                                                                                           tGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGTTG‑T‑AAAAAAGAAATGCAGTAAATAAAA‑AACGACCAGATTGCAGAGAGCAATCGTTCTCTGCAAGc            >  2:1946148/1‑140 (MQ=255)

                                                                                                                                  tttgTTCAACATAATGTTGTTTTATTAAACTATTTTACTGTTTTTTTTTCTAAATAAAAGCATCCGGTTT‑T‑AAAAAATAAATGCATTAAATAAAA‑CACGACCAGCTTGCAGAGAGCAATCGTTCTCTGCAAGCCTTtata     <  1:988520/137‑1 (MQ=255)

                                                                                                                                  ttATTTCAAACGCTTCTTTATTTAGCAAAACACTTTACTTATTTTCTTTCGAAACAAACGCATCCGGTTT‑A‑AAAAAAAAAATGCAGTAAATAAAA‑AACGACCAGCTTGCAGAGAGCAATCGTTCTCTGCAAGCCTTtata     <  1:3621711/140‑1 (MQ=255)

                                                                                                                                   tAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGTTG‑T‑AAAAAAGAAATGCAGTAAATAAAA‑AACGACCAGCTTGCCGAGAGCACTCGTTCTCTGCAAGCCTTTATaa    >  1:3469528/1‑140 (MQ=255)

                                                                                                                                   tAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGTTG‑T‑AAAAAAGAAATGCAGTAAATAAAA‑AACGACCAGCTTGCAGAGAGCAATCGTTCTCTGCAAGCCTTTATaa    >  2:1939537/1‑140 (MQ=255)

                                                                                                                                   tAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGTTG‑T‑AAAAAAGAAATGCAGTAAATAAAA‑AACGACCAGCTTGCAGAGAGCAATCGTTCTCTGCAAGCCTTTATaa    >  1:2409744/1‑140 (MQ=255)

TGTTCCATTTGCAATGGGTCATTACGTTGAAAACATCGGGGACGAACCGCTTGTCTTTTTAGAAATCTTTAAAGACGATCATTATGCCGATGTATCTTTAAACCAATGGCTTGCAATGCTTCCTGAAACATTAGTTCAACAGCATCTTGATTTAGGAAAAGACTTTACTGATATTCTTTCGAAAGAAAAGCATCCGGTTG‑T‑AAAAAAGAAATGCAGTAAATAAAA‑AACGACCAGCTTGCAGAGAGCAATCGTTCTCTGCAAGCCTTTATAAGG  >  NZ_CP034943/3219598‑3219870

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`