BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	3,851,230	G→T	5.7%	Q49K (CAG→AAG)	EO946_RS19820 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	3,851,230	0	G	T	5.7%	809.7 / 2.3	309	Q49K (CAG→AAG)	EO946_RS19820	hypothetical protein
Reads supporting (aligned to +/- strand): ref base G (201/74); new base T (2/32); total (203/106)
Fisher's exact test for biased strand distribution p-value = 1.18e-14
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 313 total aligned reads displayed.

CCTGCAATGTCCCAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCC‑CAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATT  >  NZ_CP034943/3851094‑3851369

 cTGCAATGTCCCAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATc                                                                                                                                          >  1:3172844/1‑140 (MQ=255)

  tGCAATGTCCCAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCa                                                                                                                                         <  2:2264918/140‑1 (MQ=255)

       tGTCCCAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGAtt                                                                                                                                    <  1:3478012/140‑1 (MQ=255)

       tGTCCCAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGAtt                                                                                                                                    <  1:2571454/140‑1 (MQ=255)

         tCCCAATCTGCTCTCTGTCATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTAACTGTATCATGATTCg                                                                                                                                  >  2:2009099/1‑140 (MQ=255)

         tCCCAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGACCCAGTCGTGATTACCGGTATCATGATTCg                                                                                                                                  >  1:474976/1‑140 (MQ=255)

         tCCCAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTCTCATGATTCg                                                                                                                                  >  2:76779/1‑140 (MQ=255)

         tCCCAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCg                                                                                                                                  >  1:999742/1‑140 (MQ=255)

         tCCCAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCg                                                                                                                                  >  1:1571243/1‑140 (MQ=255)

         tCCCAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCg                                                                                                                                  >  1:2057309/1‑140 (MQ=255)

           ccAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGAt                                                                                                                                <  2:852307/140‑1 (MQ=255)

           ccAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGAt                                                                                                                                <  1:2767911/140‑1 (MQ=255)

           ccAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGAt                                                                                                                                <  1:1923103/140‑1 (MQ=255)

            cAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATc                                                                                                                               <  1:2529903/140‑1 (MQ=255)

            cAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATc                                                                                                                               <  1:1507480/140‑1 (MQ=255)

            cAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATc                                                                                                                               <  2:2746006/140‑1 (MQ=255)

             aaTCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCg                                                                                                                              <  1:2070956/140‑1 (MQ=255)

             aaTCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCg                                                                                                                              <  1:2373891/140‑1 (MQ=255)

              aTCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGt                                                                                                                             >  2:1560476/1‑140 (MQ=255)

                   ctctctGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAATGTACTCTGAGAGGTCTTCTATAAATGTACCAGTCGGTCTTTCCGGTAGCATGATTCGATAGTATGCa                                                                                                                        >  1:916312/1‑140 (MQ=255)

                   ctctctGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATTCa                                                                                                                        >  1:1741472/1‑140 (MQ=255)

                   ctctctGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCa                                                                                                                        >  1:3789372/1‑140 (MQ=255)

                   ctctctGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTAGATCGTATGCa                                                                                                                        >  1:824065/1‑140 (MQ=255)

                   ctctctGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTAGATCGTATGCa                                                                                                                        >  2:1703255/1‑140 (MQ=255)

                   ctctctGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTAACTGTATAATGATTCGATCGTATGCa                                                                                                                        >  1:3386200/1‑140 (MQ=255)

                     ctctGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAg                                                                                                                      <  2:2165468/140‑1 (MQ=255)

                        tGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTAGTACATGTATCATTATTAGATCGTATGCAAGccg                                                                                                                   >  1:1392255/1‑139 (MQ=255)

                             tatGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTcc‑c                                                                                                             >  2:3180967/1‑140 (MQ=255)

                                ggATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAATCCCTTCC‑Caaa                                                                                                          >  2:2879053/1‑140 (MQ=255)

                                ggATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCC‑Caaa                                                                                                          <  1:622629/140‑1 (MQ=255)

                                ggATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCC‑Caaa                                                                                                          <  1:1025574/140‑1 (MQ=255)

                                ggATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCGTCC‑Caaa                                                                                                          >  2:511229/1‑140 (MQ=255)

                                gcagtcgAAATGGAAGTATCTTTTCGATAGTTTTATCAAGTTTTAAGCATAACCATCAAATTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTCTGCAAGCCCTTCC‑Caaa                                                                                                          <  2:2655966/134‑1 (MQ=255)

                                gcagtGGAAATGGAATTCTCTTTTCGCTAGTTTTCTCACATTGTAAGCATAACCTTCAAAGTACTCGTACAGGTCTTCTAGAATTGTACCAGGCGTTATTACCTGTCTCATGATGCGATCCTATGCAACCCCTACC‑Caaa                                                                                                          <  2:1914170/136‑1 (MQ=255)

                                  aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGAtt                                                                                                                                    >  2:1461786/1‑113 (MQ=255)

                                  aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCC‑Caaaaa                                                                                                        >  2:342836/1‑140 (MQ=255)

                                  aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCC‑Caaaaa                                                                                                        >  2:1747825/1‑140 (MQ=255)

                                  aTTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCGTCC‑Caaaaa                                                                                                        >  2:2974200/1‑140 (MQ=255)

                                    tGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCg                                                                                                                              <  1:576823/117‑1 (MQ=255)

                                    tGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCg                                                                                                                              >  2:576823/1‑117 (MQ=255)

                                    tGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCC‑CAAAAACg                                                                                                      >  1:1633760/1‑140 (MQ=255)

                                    tGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCC‑CAAAAACg                                                                                                      >  2:2839445/1‑140 (MQ=255)

                                    tGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCC‑AAAAAAcc                                                                                                      >  1:3063853/1‑139 (MQ=255)

                                       aaaTGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGAtt                                                                                                                                    >  1:1988410/1‑108 (MQ=255)

                                       aaaTGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGAtt                                                                                                                                    <  2:3725038/108‑1 (MQ=255)

                                         aTGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCC‑CAAAAACGATaaa                                                                                                 >  2:1377087/1‑140 (MQ=255)

                                            gAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCC‑CAAAAACGATAAAACg                                                                                              >  1:3011217/1‑140 (MQ=255)

                                                    ttttCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCC‑CAAAAACGATAAAACGACTCTGct                                                                                      >  1:3738830/1‑140 (MQ=255)

                                                    ttttCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTGATTAACAGTATCATGATTCGATCGTATGCAAGCCCTTCC‑CAAAAACGAAAAAACGACTCTGct                                                                                      >  1:1318924/1‑140 (MQ=255)

                                                      ttCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCC‑CAAAAACGATAAAACGACTCTGcttc                                                                                    >  2:3241896/1‑140 (MQ=255)

                                                              ttttATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCC‑CAAAAACGATAAAACGACTCTGCTTCTTCAAGtt                                                                            >  1:2994845/1‑140 (MQ=255)

                                                                     cccTTGTACGAATACCCTACAAATTACTCGCACAGGTCTTATTTAAATCACCCAGTCTTTATTACGTGTACCTTTATTCGATCGGAGGCACGCCCTCCC‑CAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGa                                                                     <  2:3707809/139‑1 (MQ=255)

                                                                     acgttttAAGCATAACCTACAAAGTACTCGGACAGGTCTTCTAAAACACAACCAGTCTTTATTACCTGTTTCTTTTTTCGATCTTATGCAAGCCCTCCC‑AAAAAACTAAAACACGACTCTGCTTCTTCAAGTTCGCAAGa                                                                     <  2:3496918/134‑1 (MQ=255)

                                                                       cTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCC‑CAAAAACGATAAAACGACTCTGCTTCTTCAACTTCGCAAGACa                                                                   >  1:3672893/1‑140 (MQ=255)

                                                                             ataaTAACATTAAAAGTACTCGGACTTTTTTTATATAAATTAACCTGTGTTTTTTACGTTTATATTGTTTCGCTCGTATGAACGCCCTCCC‑AAAAAACCAAAACACGACTCTGCTTCTTCAAGTTCGCAAGACATaacaa                                                             <  1:3650035/137‑1 (MQ=255)

                                                                                 tAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCC‑CAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtg                                                         >  2:1776690/1‑140 (MQ=255)

                                                                                 tAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCC‑CAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtg                                                         >  1:3596665/1‑140 (MQ=255)

                                                                                 tAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCC‑CAAAAACGATAAAACGACTCTGCTTATTCAAGTTCGCAAGACATAACAACAtg                                                         >  1:716389/1‑140 (MQ=255)

                                                                                  aaCCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCC‑CAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtga                                                        >  2:2429104/1‑140 (MQ=255)

                                                                                  aaCCTACAATGTACGCGGCCAGGTCTTCTATAAATCACCCTGTGTTTTTTACCTTTATCTTGATTCTACGGTCGCCACCCCCTCCC‑AAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtga                                                        <  2:1474060/140‑1 (MQ=255)

                                                                                   cccTACAAAGTACGCGCACATGTATATTCTACTTCAACCTGACGTTCTTACCTGTATAATGATTCGCACGTAAGCAACCCCTGCC‑CAAAACCCATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATCACAACTtgat                                                       <  2:3822365/139‑1 (MQ=255)

                                                                                    ccTTCACAGTACAGGCACTGGTCTTCTATAATTTAACCAGTCTTTTTTACCTTTATCATTATACGAGCGTATGAAACCCCTTCA‑AAAAAACTATAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtgatg                                                      <  2:1306552/140‑1 (MQ=255)

                                                                                    ccTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGTTTCGATCGTATGCAAGCCCTTCC‑CCAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtgatg                                                      >  1:2124666/1‑140 (MQ=255)

                                                                                    ccTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCC‑CAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtgatg                                                      >  1:1796253/1‑140 (MQ=255)

                                                                                    cattCAAATTACTCGAACAGTTCTTATATAAATCAACCTGTCTTTATTCCCTGTATCATGATTCGATCGTATGCAACCCCTTCC‑CAAAAACGAAAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTtgatg                                                      <  2:3018963/138‑1 (MQ=255)

                                                                                         aaaGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCC‑CAAAAACGATAAAAAGACTCTGCTTCTTCAAGTTCGCAAGACATAAAAACTTGATGAGGCt                                                 >  2:3214534/1‑140 (MQ=255)

                                                                                          aaGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCC‑CAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCtt                                                >  2:3280356/1‑140 (MQ=255)

                                                                                               cTCGGACTGTTTTTCTTTAAATCAACCAGTCGTTATCACCTGTTTCATTATGCGTTCGTAGGCACGCCGTCCC‑CAAAAACGTTAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAagag                                           <  2:2504934/140‑1 (MQ=255)

                                                                                               cTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCC‑CAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAAAGTGATGAGGCTTAagag                                           >  1:285857/1‑140 (MQ=255)

                                                                                               cTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCC‑CAAAAACGATAAAAAGACTCTGCTTCTTCAAGTTCGCAACACATAACAACTTTATGAGGCTTAagag                                           >  1:1853845/1‑140 (MQ=255)

                                                                                               cTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTGCC‑CAAAAAAGATAAAACGACTCAGCTTCTTCAAGTTCGCCAGACAGAACCACTTGAAGAGGCTTAacag                                           >  1:2237331/1‑140 (MQ=255)

                                                                                               cTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGAGTCGATCGTATGCAAGCCCTTCC‑CAAAAACGATAAAACGACTCTGCTTCTTAAAGTTCGCAACACATAACAACTTGATGAGGCGTAacag                                           >  2:788309/1‑140 (MQ=255)

                                                                                               cTCGGACAGGTCTGCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCC‑CAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACCTAACAACGTGAGTAGGCGTAagat                                           >  2:840442/1‑139 (MQ=255)

                                                                                                tggcaCAGGTTTTCTAAAAATGAACCAGTCGTTTTTACATTTATCTTTATGCGTTCGTTTCCACGCCCCTCCAAAAAAACGATAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAagag                                           <  2:1661574/136‑1 (MQ=255)

                                                                                                tCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCC‑CAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGc                                          >  2:3355654/1‑140 (MQ=255)

                                                                                                tCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCC‑CAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAAAAACTTGATGAGGCTTAAGAGc                                          >  2:1023906/1‑140 (MQ=255)

                                                                                                       ggTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCC‑CAAAAACGATAAAACGACTGTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGATCGATCATg                                   >  2:2456388/1‑140 (MQ=255)

                                                                                                       ggTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCC‑CAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATg                                   >  1:3842889/1‑140 (MQ=255)

                                                                                                       ggTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCC‑CAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATg                                   >  2:418511/1‑140 (MQ=255)

                                                                                                       ggTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCC‑CAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGAGATCATg                                   >  2:236655/1‑140 (MQ=255)

                                                                                                       ggTCTTCTATAAATGAACCAGTCGTTATTAACTGTATCATGATTAGATCGTATGCAAGGCCTTCC‑CAAAAACGATAAAACGACTCTGCTTCTTCAATTTAGGCACACATAACAACTTGATGAGGCTTAATATCGAACATg                                   >  1:2727853/1‑140 (MQ=255)

                                                                                                            tctATAAATCAACCTGTCGTTATTACCTTTATCATGATTCGATGTTATGCACGCCCTTCC‑AAAAAACCATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTaa                              <  2:30679/140‑1 (MQ=255)

                                                                                                            tatataAATGAACCATGCTTTATTCCCTGTATCATGATTCGAGCGTATGCAAGCCCTTCC‑AAAAAACCATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTaa                              <  2:632883/138‑1 (MQ=255)

                                                                                                                 aaaTTAACCAGTGTTTATTCCCTTTATCTTTTTTCGATCGTCTCCAAGCCCTCCC‑AAAAAACTAAACCACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGc                         <  1:262443/140‑1 (MQ=255)

                                                                                                                 aaaTCAACCATTCGTTATTACGTTTATCATTATTCGATCGTATGCACCCCCTTCC‑CAAAAACGAAAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGc                         <  2:495195/140‑1 (MQ=255)

                                                                                                                  aaTGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCC‑CAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGcc                        >  2:1191401/1‑140 (MQ=255)

                                                                                                                   aTGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCC‑CAAAAACGATAAAACGACTCTGCTTCTTCAACTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCg                       >  2:712183/1‑140 (MQ=255)

                                                                                                                   aTGAACCAGTCGTGATTACCTGTATCATGATTCGATCCTATGCAAACCCATAC‑CAAAAACGATAAAACGACTCTGCTTCATCAAGTTCGCAAGACATAACAACAAGATGAGGCTTAACACCGATCATGGCTAAATTGCCg                       >  2:1269979/1‑140 (MQ=255)

                                                                                                                      caCCAGGCGTTTTTACCTTTATAATGATGCGAGGTTCGGAAACCCTTTCC‑CAAAAACCATAACACGACTGTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATa                    <  2:410059/139‑1 (MQ=255)

                                                                                                                      aaCCAGTCTTTTTTACCTTTATCATGATTCTCTCTTATGAAACCCCTCCC‑AAAAAACGAAAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATa                    <  1:2679807/140‑1 (MQ=255)

                                                                                                                       aCCAGTCGTTATTACCTGTATCATGATTAGATCGTATGCAAGCCCTTCC‑CAAAAACGATAAAACGACACTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATAc                   >  1:89870/1‑140 (MQ=255)

                                                                                                                          aGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCC‑CAAAAACGATACAACGACTCTCCTTCTTCAAGTTAGCAAGACATAACAACTTGATGAGGCATAAGAGCGATCATGTCAAAATTCCCGATACTGc                >  2:3116906/1‑140 (MQ=255)

                                                                                                                            tCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCC‑CAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACAAAACAACATGATGATGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCtt              >  1:1108878/1‑140 (MQ=255)

                                                                                                                                ttttaCCTTTATCATGATTCCCTCTTATCCAAGCCCTACC‑AAAAAACGAAAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAg          <  2:26119/138‑1 (MQ=255)

                                                                                                                                tattaCGTGTTTCATTTTTCGATCTTATGCAAGCCCTTCC‑AAAAAAGTATAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAg          <  2:3768334/140‑1 (MQ=255)

                                                                                                                                      cTGTATCATGATTCGATCGTATGCAAGCCCTTCC‑CAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTa    >  1:3267989/1‑140 (MQ=255)

                                                                                                                                       tGTATCATGATTCGCTCGTATGCACCCCCTTCC‑CAAAAACTATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTAt   <  2:3596665/140‑1 (MQ=255)

                                                                                                                                       tGTATCATCATTCGACCGTATCCAAGCCCTTCC‑CAAAAACCATAACACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTAt   <  2:2023087/140‑1 (MQ=255)

                                                                                                                                        gTATCATGATTCGATCGTATGCAAGCCCTTCC‑CAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTAtt  >  2:2822363/1‑140 (MQ=255)

                                                                                                                                        gTATCATGATTCGATCGTATGCAAGCCCTTCC‑CAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTAtt  >  1:3497973/1‑140 (MQ=255)

CCTGCAATGTCCCAATCTGCTCTCTGTAATATGGATTGGAAATGGAATTCTCTTTTCGCTAGTTTTATCACCTTGTAAGCATAACCTTCAAAGTACTCGGACAGGTCTTCTATAAATGAACCAGTCGTTATTACCTGTATCATGATTCGATCGTATGCAAGCCCTTCC‑CAAAAACGATAAAACGACTCTGCTTCTTCAAGTTCGCAAGACATAACAACTTGATGAGGCTTAAGAGCGATCATGTCTAAATTGCCGATACTGCTTATAGCGGTTATT  >  NZ_CP034943/3851094‑3851369

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`