BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	3,996,926	T→A	7.2%	N132Y (AAT→TAT)	EO946_RS20530 ←	VOC family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	3,996,926	0	T	A	7.2%	650.8 / 5.9	235	N132Y (AAT→TAT)	EO946_RS20530	VOC family protein
Reads supporting (aligned to +/- strand): ref base T (110/102); new base A (2/17); total (113/122)
Fisher's exact test for biased strand distribution p-value = 4.93e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 242 total aligned reads displayed.

TAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCC‑TTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAACCTCTTGATAGCCCATGTGTTTTAGCTTTGAAGTGATCGCTGTCAGTTTGTCTGCATTCGGC  >  NZ_CP034943/3996790‑3997065

taaattaaTGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCC‑TTTTGTATTCAt                                                                                                                                          >  2:1883462/1‑140 (MQ=255)

      aaTTTAAATAACTATTCGTTCGCTTCCATTCTTTTTACAAGCGTTTTCTCATCGCATTGTCATAAAAAGCTCGTCAAACTTTTATCCTTTAGCACTAAAAAGAGTTACTTGCCTGATATCCC‑TTTTGTATTCATAAAAAc                                                                                                                                    <  2:1985655/140‑1 (MQ=255)

        tGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCC‑TTTTGTATTCATAAAAACGa                                                                                                                                  >  1:2870812/1‑140 (MQ=255)

        tGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCC‑TTTTGTATTCATAAAAACGa                                                                                                                                  >  1:3404714/1‑140 (MQ=255)

        tGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCC‑TTTTGTATTCATAAAAACGa                                                                                                                                  >  2:250439/1‑140 (MQ=255)

          taaataaaTATTCGTTCGATACAATTGGTTTAAAAAGCGTTTTCTCATCGATTTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGATTTACTTCCCTGATATCCC‑TTTTTTATTCATAAAAACGACt                                                                                                                                <  2:3086184/140‑1 (MQ=255)

               cattatGCGTTGGCTCCCATAGTTTTTAAACGGGTTTTCTCATCGCTTTGTCCTAAAAAGCGCGTGAAACTTTTATCCTTTCGCACTAAAAAGAGTTAGTTGCCTTATAGCCC‑TTTGTTTTTCATAAAAACGACTCGCCa                                                                                                                           <  2:1632515/137‑1 (MQ=255)

                        ttcgCTTCCATTCTTTTTAAAAGCGTTTTATCATCGCTTTGTACTAAAAAGCTCTTTAAAATTTTATCCTTTAGCACTAAAAATAGTTACTTGCCTTATATCCC‑TTTTTTATTCATAAAAACGACTCGCCAACCGTCCgg                                                                                                                  <  2:394901/140‑1 (MQ=255)

                        ttcgCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCC‑TTTTGTATTCATAAAAACGACTCGCCAACCGTCCgg                                                                                                                  >  1:3146630/1‑140 (MQ=255)

                        ttcgCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCC‑TTTTGTATTCATAAAAACGACTCGCCAACCGTCCgg                                                                                                                  >  1:976575/1‑140 (MQ=255)

                        ttcgCTTCAATTCGTTTTACATGCGTTTGCTCACCGCATTGTCCTAAAACCCCCTAGAAAATTTTATCCTTTAGCACTAAAAATAGTTTCTTGCCTCATATCCCTTTTTGTA‑TAAAAAACACGACTCGCCAACCGTCCgg                                                                                                                  <  2:1056051/140‑1 (MQ=255)

                         tcgCTTCCTTTCTTTTTACATGCGTTTTCTCATCGCTTTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACTAAAAAGATTTAGTTGCCTGATATCCC‑TTTTGTTTTCATAAACACGACTCGCCAACCGTCCGGa                                                                                                                 <  2:1384062/140‑1 (MQ=255)

                         tcgCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCC‑TTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGa                                                                                                                 >  2:1741802/1‑140 (MQ=255)

                            cTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCC‑TTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATcc                                                                                                              >  1:1459248/1‑140 (MQ=255)

                            cTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCC‑TTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATcc                                                                                                              >  2:814504/1‑140 (MQ=255)

                            cTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCC‑TTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATcc                                                                                                              >  1:511428/1‑140 (MQ=255)

                             gtCCATTAGTTTTACAAGCGTTTTCTCAGCGATTTTTCCAAAAAAGCTCTTTAATCTTTTATCTTTTAGCACGAAAAAGAGTTACTGGCTTGATATCCC‑TTTTGTTTTCAAAAAAACGACTCGCCAACCGTCCGGATCct                                                                                                             <  2:3374084/139‑1 (MQ=255)

                                    ctttttACAAGCTTTTTCTATTCTCTTTGTCCTAAACAGCTCGTCAAAATTTTATCCTTAAGCACCAAAAAGAGTTACTTGCCTGATACCCC‑TTTTGTTTTAATAAACACGACTCGCCAACCGTCCGGATCCTCTATCGt                                                                                                      <  1:3005663/138‑1 (MQ=255)

                                      ttttAAAAGCGTTTTCTCATCGCATTTTCATAAAAACCTCTTTAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTTATATCCG‑TTTTTTATTCAAAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCa                                                                                                    <  2:1277392/140‑1 (MQ=255)

                                      tttaACACGCGTTTCCACAGCGCTTGGTCCCAAACACCTCGAGACAATTTAACCCTTTAGCACCAAAACCATTTAGTGGCCGTATATCCC‑TTTTGTATTCAAAAAAACGACTCGCCAACCGTGCGGATCCTCTATCGTCa                                                                                                    <  1:3505131/140‑1 (MQ=255)

                                       tttACATGCTTTTTCTCATGGCTTTTTCCTAAAAAGCCCGTGAAACTTTTATCCTTTAGAACTAAAAAGAGTTAGTTTCCTTATATCCC‑TTTTGTTTTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCAc                                                                                                   <  2:262650/140‑1 (MQ=255)

                                       tttACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCC‑TTTTGTATTCATAAAAACTACTCGCCAACCGTCCGGATCCTCTATCGTCAc                                                                                                   >  1:448830/1‑140 (MQ=255)

                                          acacGCGTTTTCACATGGAATTTTCATAAAAAGCTCGTGAAACTTTTATCCTTTAGCACTAAAAAGAGTTACTTGCCTCTTAGCCC‑TTTTTTATTAATAAACACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGcc                                                                                                <  1:3666960/140‑1 (MQ=255)

                                           cAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCC‑TGTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCt                                                                                               >  2:1345761/1‑140 (MQ=255)

                                             aGCGTTTTCTCATCGCTTTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACTAAAAAGAGTTAGTTGCCTGATATCCC‑TTTTGTATTCATAAACACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTcc                                                                                             <  2:1024664/140‑1 (MQ=255)

                                              gCGTTTTAACATCGCATTGTCCAAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGTTTTCCC‑TTTTGTATTAATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCa                                                                                            <  2:601753/140‑1 (MQ=255)

                                               gtttttCTCATCGATTTGTACTAAAAAGCTGGTCAAATTTTTATCCTTTACCACTAAAAAGTTTTAGTGGCCTGATACCCG‑TTTTTTTTTAAAAAACACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCAt                                                                                           <  1:1019653/138‑1 (MQ=255)

                                               ggTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAAGTTTTATCCTTTAGCACTAAAAAGAGTTACTTGCCTGATATCCC‑TTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCAt                                                                                           <  2:2728209/139‑1 (MQ=255)

                                               ggTTTTATCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTCGCACTAAAAAGAGTTAGTTGACTGATATCCC‑TTTTGTATTAATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCAt                                                                                           <  2:2756825/139‑1 (MQ=255)

                                               ctttttATCATCGAATTGTCCTAAAAAGCTCTTTAAACTTTTATCCTTTACCACCAAAATTTGTTACTTGCGTTATATCCC‑TTTTTTATCCAAAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCAt                                                                                           <  1:1741802/138‑1 (MQ=255)

                                                 ttttCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCC‑TTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATgg                                                                                         >  1:2767231/1‑140 (MQ=255)

                                                  tttCTCTTCGATTTTTCATAAAAAGCTCTTTAAATTTTTATCATTTACAACTAAAAATATTTATTTGCCTTATATCCC‑TTTTTTTTTAAAAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGc                                                                                        <  1:2109064/140‑1 (MQ=255)

                                                  ttactcAGCGAATTTTCAAAAAAAGCTCTTCAAATTTTTATCCTTTAGCACTAAAAATATTTCGTTGCGTTATATCCT‑TTTTGTTTTAAAAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGc                                                                                        <  1:3172186/137‑1 (MQ=255)

                                                          cGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCC‑TTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTAc                                                                                >  1:2277388/1‑140 (MQ=255)

                                                          cGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCC‑TTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTAc                                                                                >  1:1658536/1‑140 (MQ=255)

                                                                 cacaaaaacGCTCGTCAAACTTTTATCCTGTCCCCCTAAAACTAGTTACTTGCCTTTTAACCC‑TTTTTTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTtct                                                                         <  1:1174968/136‑1 (MQ=255)

                                                                      aaaaGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCC‑TTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGAt                                                                    >  2:205025/1‑140 (MQ=255)

                                                                        atgcgcGTTAAAGTTTTATCGTTTCGCCCCAAAAAGTGTTATTTGCCTGATAGCCC‑TTTTGTTTTCTTAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTc                                                                  <  2:2926627/135‑1 (MQ=255)

                                                                        acgCTGGTGAAAGTTTTTTCCTTTAGCACCAAAAATTTTTTGTGGCCTCTTAGCCT‑TTTTGTTTACAAAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTc                                                                  <  1:1485096/138‑1 (MQ=255)

                                                                        aaccTCGTGAAACTTTTATCCTTTCGCACTAAAACGATTGACTTGCCTGATAGCCC‑TTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTc                                                                  <  1:2654420/137‑1 (MQ=255)

                                                                        aaGCGCGTCAAACTTTAATCCTTTACCACTAAAAATAGTTACTTGCCTGATATCCG‑TTTTGTTTTCAAAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGGTCTCAGATTc                                                                  <  2:71473/140‑1 (MQ=255)

                                                                          gCTCGTGAAACTTTTTTCCTTTAGCACGAAAAGGTGTTAATTGCCTGATATGCC‑TTTTTTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCaa                                                                <  2:2870875/140‑1 (MQ=255)

                                                                           cgcGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCC‑TTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACg                                                                                                  <  2:3746997/103‑1 (MQ=255)

                                                                           cTCGTTAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCC‑TTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAAc                                                               <  2:2795195/140‑1 (MQ=255)

                                                                           cTCGTGAAACTTTTATCCTTTAGCACGAAAAATAGTTACTTGCCTGATATCCC‑TTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCCGTACGGGGTCTCAGATTCAAc                                                               >  1:1983305/1‑140 (MQ=255)

                                                                           cTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTAGTTGCCTGATATCCC‑TTTTTTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAAc                                                               <  2:1255525/140‑1 (MQ=255)

                                                                           cTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCC‑TTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGc                                                                                        <  2:3246158/115‑1 (MQ=255)

                                                                           cTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCC‑TTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGc                                                                                        >  1:3246158/1‑115 (MQ=255)

                                                                           cTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCC‑TTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAAc                                                               >  2:1608225/1‑140 (MQ=255)

                                                                           cTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCC‑TTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAAc                                                               >  1:1133519/1‑140 (MQ=255)

                                                                           cTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCC‑TTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAAc                                                               >  2:3037592/1‑140 (MQ=255)

                                                                           cTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCC‑TTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAAc                                                               >  2:2702442/1‑140 (MQ=255)

                                                                           cTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCC‑TTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAAc                                                               >  1:790253/1‑140 (MQ=255)

                                                                           cTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCC‑TTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAAc                                                               >  1:2410900/1‑140 (MQ=255)

                                                                           cTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCC‑TTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAAc                                                               >  1:3739028/1‑140 (MQ=255)

                                                                           cTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCC‑TTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAAc                                                               >  2:3310818/1‑140 (MQ=255)

                                                                           cTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCC‑TTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAAc                                                               >  1:3596253/1‑140 (MQ=255)

                                                                           cTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCC‑TTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAAc                                                               >  1:262688/1‑140 (MQ=255)

                                                                           cTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCC‑TTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAAc                                                               >  1:2994927/1‑140 (MQ=255)

                                                                           cTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCC‑TTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAAc                                                               >  1:3311811/1‑140 (MQ=255)

                                                                           cTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCC‑TTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATAGCTCCAGTACGGGTTCTCAGATTCAAc                                                               >  1:1009667/1‑140 (MQ=255)

                                                                           cTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCC‑TTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCCCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAAc                                                               >  1:3196484/1‑140 (MQ=255)

                                                                           cTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCC‑TTTTGTATTCATAAAAACGACTCGCCAACAGTCCGGATCCTCTATAGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCatc                                                               >  2:2223347/1‑138 (MQ=255)

                                                                           cTCGTGAAACTTTTATCATTTAGCACGAAAAAGAGTTACTTGCATTATATCCC‑TTTTGTATTCATAAAAACGACTCGCCAACCGTCAGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCATATTCaaa                                                               >  2:1933087/1‑139 (MQ=255)

                                                                            tCGTGAAAGTTTTACCAGTTACCACTAAAACCCGTTACTTCCCTCTTCGCCC‑TTTTGTTTTAATAAACACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCGCCATCGCTCCAGTACGGGTTCTCAGATTCAAcc                                                              <  1:396103/140‑1 (MQ=255)

                                                                             ctttaaaTTTTTTTCCTTTCTCACTAAAAATATTTACTTGCCTTATATCCT‑TTTTTTTTTAATAAAAACGACTCGCCAACCGTCCGTATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTAAACct                                                             <  2:1514303/136‑1 (MQ=255)

                                                                                   aCTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTTATATCCA‑TTTTGTATACAAAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAACCTCTTGAt                                                       <  1:2714798/140‑1 (MQ=255)

                                                                                         agccTTCACCACCAAAAAGAGGTAGTTGCCTGATATCCG‑TTTGGTATACAAAAACACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAACCTCTTGATAGCCCa                                                 <  1:2289146/138‑1 (MQ=255)

                                                                                         aTCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCC‑TTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAACCTCTTGATAGCCCa                                                 <  2:890730/140‑1 (MQ=255)

                                                                                          tCCTTTCCCACCAAAACTATTTACTTGCCTCATATCCC‑TTTTGTATTAATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAACCTCTTGATAGCTCAt                                                <  1:250439/140‑1 (MQ=255)

                                                                                          cccTTTACCACCAAAAACATTTACTTGCCTTATATCAC‑TTTTCTATTCAAAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAACCTCTTGATAGCCCAt                                                <  2:3689190/139‑1 (MQ=255)

                                                                                          accttgcgCACGAAAAACTTTTACTTGCCGGATACCCC‑TTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAACCTCTTGATAGCCCAt                                                <  2:459326/133‑1 (MQ=255)

                                                                                               tAGCACGAAAAAGAGTTACTTGCCTGATATCCC‑TTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAACCTCTTGATAGCCCATGTGtt                                           >  2:1944397/1‑140 (MQ=255)

                                                                                               tAGCACGAAAAAGAGTTACTTGCCTGATATCCC‑TTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAACCTCTTGATAGCCCATGTGtt                                           >  1:2645774/1‑140 (MQ=255)

                                                                                               tAGCACGAAAAAGAGTTACTTGCCTGATATCCC‑TTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAACCTCTTGATAGCCCATGTGtt                                           >  1:2803102/1‑140 (MQ=255)

                                                                                                cgCATGAAAAATTGTTACTTGCCTTTTACCCA‑TTTTTTTTTCATAACAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAACCTCTTGATAGCCCATGTGttt                                          <  1:3753059/139‑1 (MQ=255)

                                                                                                cccACGAAAAAGATTTATTTCCCTTATAGCCC‑TTTTGTAGTCATAAACACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAACCTCTTTATAGCCCATGTGttt                                          <  1:746229/138‑1 (MQ=255)

                                                                                                cccACCAAAAAGAGTTACTGGCCTGATAGCCC‑TTTTTTATTCAAAAACACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAACCTCTTGATAGCCCATGTGttt                                          <  1:3452000/138‑1 (MQ=255)

                                                                                                ccacccaaaaaTATTTAGTGGCCTCATATCCC‑TTTTTTATAAAAAAACACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAACCTCTTGATAGCCCATGTGttt                                          <  1:1304265/134‑1 (MQ=255)

                                                                                                accACCAAAAAGAGTTCCTGGCCTTATATCCC‑TTTGGTATACAAAAACACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAACCTCTTGATAGCCCATGTGttt                                          <  1:2459778/138‑1 (MQ=255)

                                                                                                acaactaaaaaGATTTCTTTGCCTGATATCCA‑TTTTGTTTTCAAAAACACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAACCTCTTGATAGCCCATGTGttt                                          <  1:2945252/134‑1 (MQ=255)

                                                                                                     caaaatGAGTTAGTGGCCTGATATCCC‑TTTTGTCTTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAACCTCTTGATAGCCCATGTGTTTTAGCt                                     <  2:1114337/139‑1 (MQ=255)

                                                                                                         aactgTTACTGGCCTCATATCCC‑TTTTTTTTTCATAAACACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAACCTCTTGATAGCCCATGTGTTTTAGCTTTGa                                 <  1:2086961/136‑1 (MQ=255)

                                                                                                             gTTACTTGCCTGATATCCC‑TTTGGTATTCATAAACACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAACCTCTTGATAGCCCATGTGTTTTAGCTTTGAAGTg                             <  2:2414676/140‑1 (MQ=255)

                                                                                                             gTTACTTGCCTGATATCCC‑TTGTGTATTAATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCAGGGCTCCAGTACGGGTTCTCAGATTCAACCTCTTGATAGCCCATGTGTTTTAGCTTTGAAGTg                             <  2:2546315/140‑1 (MQ=255)

                                                                                                                aCTTGCCTGATATCCC‑TTTTGTATACATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAACCTCTTGATAGCCCATGTGTTTTAGCTTTGAAGTGATc                          >  2:1042456/1‑140 (MQ=255)

                                                                                                                     ccTCATATCCC‑TTTTGTATTCATAAACACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAACCTCTTGATAGCCCATGTGTTTTAGCTTTGAAGTGATCGCTGt                     <  2:59536/140‑1 (MQ=255)

                                                                                                                            tCCC‑TTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGAGTCAACCTCTGGATAGCCCATGTGTTTGAGCTTTGAAGTGATCGCTGTCAGTTTg              >  1:2592840/1‑140 (MQ=255)

                                                                                                                               c‑TTTTTTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAACCTCTTGATAGCCCATGTGTTTTAGCTTTGAAGTGATCGCTGTCAGTTTGTCt           <  2:1725117/140‑1 (MQ=255)

                                                                                                                               c‑TTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAACCTCTTGATAGCCCATGTGTTTTAGCTTTGAAGTGATCGCTGTCAGTTTGTCt           >  1:928683/1‑140 (MQ=255)

                                                                                                                               c‑TTTTGTATTAAAAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAACCTCTTGATAGCCCATGTGTTTTAGCTTTGAAGTGATCGCTGTCAGTTTGTCt           <  1:40034/140‑1 (MQ=255)

                                                                                                                                    tGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAACCTCTTGATAGCCCATGTGTTTTAGCTTTGAAGTGATCGCTGTCAGTTTGTCTGCAt       >  1:1773314/1‑140 (MQ=255)

                                                                                                                                    tGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAACCTCTTGATAGCCCATGTGTTTTAGCTTTGAAGTGATCGCTGTCAGTTTGTCTGCAt       >  1:1047007/1‑140 (MQ=255)

                                                                                                                                    tGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGCCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAACCTCTTGATAGCCCATGTGTTTTAGCTTTGAAGTGATCGCTGTCCGTTTGTCTGCAt       >  1:1115445/1‑140 (MQ=255)

                                                                                                                                        ttCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAACCTCTTGATAGCCCATGTGTTTTAGCTTTGAAGTGATCGCTGTCAGTTTGTCTGCATTCgg   <  2:350847/140‑1 (MQ=255)

                                                                                                                                        ttCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAACCTCTTGATAGCCCATGTGTTTTAGCTTTGAAGTGATCGCTGTCAGTTTGTCTGCATTCgg   <  2:1486453/140‑1 (MQ=255)

                                                                                                                                        ttCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAACCTCTTGATAGCCCATGTGTTTTAGCTTTGAAGTGATCGCTGTCAGTTTGTCTGCATTCgg   <  2:788047/140‑1 (MQ=255)

                                                                                                                                         tCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAACCTCTTGATAGCCCATGTGTTTTAGCTTTGAAGTGAt                           >  2:385133/1‑115 (MQ=255)

                                                                                                                                         tCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAACCTCTTGATAGCCCATGTGTTTTAGCTTTGAAGTGAt                           <  1:385133/115‑1 (MQ=255)

                                                                                                                                         tCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAACCTCTTGATAGCCCATGTGTTTTAGCTTTGAAGTGATCGCTGTCAGTTTGTCTGCATTCGGc  >  2:3001958/1‑140 (MQ=255)

TAAATTAATGTAAATCAATATTCGTTCGCTTCCATTCGTTTTACAAGCGTTTTCTCATCGCATTGTCCTAAAAAGCTCGTGAAACTTTTATCCTTTAGCACGAAAAAGAGTTACTTGCCTGATATCCC‑TTTTGTATTCATAAAAACGACTCGCCAACCGTCCGGATCCTCTATCGTCACGCCTCCATGGCTCCAGTACGGGTTCTCAGATTCAACCTCTTGATAGCCCATGTGTTTTAGCTTTGAAGTGATCGCTGTCAGTTTGTCTGCATTCGGC  >  NZ_CP034943/3996790‑3997065

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`