BRESEQ :: Evidence

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	2,991,227	0	T	G	27.6%	272.0 / 53.4	182	intergenic (+28/‑179)	gbsR/bslA	transcriptional regulator GbsR/biofilm‑surface layer protein BslA
Reads supporting (aligned to +/- strand): ref base T (50/81); new base G (50/0); total (100/82)
Fisher's exact test for biased strand distribution p-value = 8.04e-17
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 188 total aligned reads displayed.

ATACGAGCAACTGGAAAGCTCTAAAGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAA‑ACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACT‑AC‑AAAT‑TTGAAAAAT‑ACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCGGGCA  >  NZ_CP034943/2991091‑2991365

ataCGAGCAACTGGAAAGCTCTAAAGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAA‑ACGCCTGGGAAACCGGGCgttgtt                                                                                                                                             >  1:4434021/1‑140 (MQ=255)

ataCGAGCAACTGGAAAGCTCTAAAGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAA‑ACGCCTGGGAAACCGGGCGttttt                                                                                                                                             >  1:2267693/1‑140 (MQ=255)

   cGAGCAACTGGAAAGCTCTAAAGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAA‑ACGCCTGGGAAACCGGGCGTTGTGTTg                                                                                                                                          >  2:1348141/1‑140 (MQ=255)

   cGAGCAACTGGAAAGCTCTAAAGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAA‑ACGCCTGGGAAACCGGGCGTGTTTTTg                                                                                                                                          >  1:1725351/1‑140 (MQ=255)

     cccAACGGGAAAGCTCTAAAGCTTACTAGGATGGGCTGAACCGGCTTGCTGACTCGGTGCCGAGCGGGGAAATTTTTAAATTTATCCCGATTGAAACAACGTAAAACAAAC‑CCGCCTGGGAAACCGGGCGTTTTTTTGtt                                                                                                                                        <  1:4282818/138‑1 (MQ=255)

     aGCAACTGGAAAGCTCTAAAGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAA‑ACGCCTGGGAAACCGGGCGTTTTTTTGtt                                                                                                                                        >  2:1844539/1‑140 (MQ=255)

     aGCAACTGGAAAGCTCTAAAGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAA‑ACGCCTGGGAAACCGGGCGTTTTTTTGtt                                                                                                                                        >  2:24433/1‑140 (MQ=255)

     aGCAACTGGAAAGCTCTAAAGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAA‑ACGCCTGGGAAACCGGGCGTGTTTTTGtt                                                                                                                                        >  1:3361189/1‑140 (MQ=255)

      gCAACTGGAAAGCTCTAAAGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAA‑ACGCCTGGGAAACCGGGCGTgttgttgttg                                                                                                                                       >  1:2144553/1‑139 (MQ=255)

      gCAACTGGAAAGCTCTAAAGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAA‑ACGCCTGGGAAACCGGGCGTTTTTTTGttg                                                                                                                                       >  2:3738816/1‑139 (MQ=255)

        aaCTGGAAAGCTCTAAAGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAA‑ACGCCTGGGAAACCGGGCGTTTTTTTgttgtt                                                                                                                                     >  2:1084494/1‑140 (MQ=255)

          cTGGAAAGCTCTAAAGCTTACTATTATGGGCTCAACCGGCTTGCTCAATCGGTGCAGCGCGGGTAAATTTTTAAATTTATCCCGATTCAAACAAAGAAAAACAAAC‑ACGCCTGGGAAACCGGGCGTTTTTTTGTTCtttt                                                                                                                                   <  1:2629712/140‑1 (MQ=255)

          cTGGAAAGCTCTAAAGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAA‑ACGCCTGGGAAACCGGGCGTGttgttggtgtgtt                                                                                                                                   >  1:2011462/1‑133 (MQ=255)

          cTGGAAAGCTCTAAAGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAA‑ACGCCTGGGAAACCGGGCGTGTGTTTGTTGtttt                                                                                                                                   >  1:642977/1‑140 (MQ=255)

                  ctctAAAGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAA‑ACGCCTGGGAAACCGGGCGTGTTTTTTTTGttttttttttta                                                                                                                           >  1:2787353/1‑134 (MQ=255)

                  ctctAAAGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAA‑ACGCCTGGGAAACCGGGCGTGTTTTTTTTCTTGTTTTTTTaa                                                                                                                           >  2:4042906/1‑140 (MQ=255)

                   tctAAAGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAA‑ACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAAt                                                                                                                          <  1:1976978/140‑1 (MQ=255)

                    ctAAAGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAA‑ACGCCTGGGAAACCGGGCGTTTTTTTGTTGTTTTTTATTTAAtt                                                                                                                         >  2:2776452/1‑140 (MQ=255)

                     tAAAGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCTGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAA‑ACGCCTGGGAAACCGGGCGTTTTTTTGTTATTTTTTATTTAATta                                                                                                                        >  2:2055659/1‑140 (MQ=255)

                     tAAAGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAA‑ACGCCTGGGAAACCGGGCGTTTTTTTTTTATTTTTTATTTAATta                                                                                                                        >  2:1300097/1‑140 (MQ=255)

                     tAAAGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAA‑ACGCCTGGGAAACCGGGCGTTTTTTTGTTGTTTTTTATGTAATta                                                                                                                        >  2:2566303/1‑140 (MQ=255)

                     tAAAGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAA‑ACGCCTGGGAAACCGGGCGTTGTTTTGTTGTTTTTTTTGTAAttt                                                                                                                        >  2:3001858/1‑139 (MQ=255)

                     tAAAGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAA‑ACGCCTGGGAAACCGGGCGTGTTTTTTTTTTTTTTTTTGTAAttt                                                                                                                        >  2:3692776/1‑139 (MQ=255)

                     tAAAGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAA‑ACGCCTGGGAAACCGGGCGTGTTTTTGTTGTTTTTTATGTAATta                                                                                                                        >  2:4665366/1‑140 (MQ=255)

                            tACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAA‑ACGCCTGGGAAACCGGGCGTGTTTTTTTTGTTTTTTATGTAATTAAT‑AC‑aaa                                                                                                               >  1:4555152/1‑140 (MQ=255)

                            tACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAA‑AAGCCTGGGAAACCGGGCGTTTTTTTTTTGTTTTTTATGTAATTAAA‑AC‑aaa                                                                                                               >  2:1516405/1‑140 (MQ=255)

                            tACTATGATTGGCTGAAACGGATTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAA‑AAGCCTGGGAAACCGGGCGTTTTTTTGTTGTTTTTTATGTAATTACT‑AC‑aaa                                                                                                               >  2:2486838/1‑140 (MQ=255)

                             aCTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAA‑ACGCCTGGGAAACCGGGCGTTTTTTTTTTGTTTTTTATGTAAATACT‑AC‑AAAt                                                                                                              >  2:386/1‑140 (MQ=255)

                             aCTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAA‑ACGCCTGGGAAACCGGGCGTGTTTTTTTTGTTTTTTTTTTTATTTAT‑AC‑aaaa                                                                                                              >  1:2471466/1‑139 (MQ=255)

                             aCTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAA‑ACGCCTGGGAAACCGGGCGTGTTTTTTTTGTTTTTTATTTTATTAAT‑AA‑AAAt                                                                                                              >  2:4158856/1‑140 (MQ=255)

                             aCTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAA‑ACGCCTGGGAAACCGGGCGTGTTTTTGTTGTGTTTTATGTAATTAAT‑AC‑CAAt                                                                                                              >  2:3986184/1‑140 (MQ=255)

                             aCTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAA‑ACGCCTGGGAAACCGGGCGTGTTTTTGTTATTTTTTATTTAATTAAT‑Aa‑aaaa                                                                                                              >  1:1573621/1‑139 (MQ=255)

                                ttGATTGGGTGAACCGGCTGGCTCAATCGGTGCCGAGCGGGGAATTTTTTAAATTTATACCGATTTAAACAAAGTAAAACAAAA‑ACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACT‑AC‑AAAT‑TTg                                                                                                          <  1:4664678/139‑1 (MQ=255)

                                aTGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAA‑ACGCCTGGGAATCCGGGGGTTTGTTTGGTGTTGTTTTTGTTGGTTCT‑AC‑AAAT‑TTg                                                                                                          >  1:3706889/1‑140 (MQ=255)

                                aTGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAA‑ACGCCTGGGAAACCGGGCGTGTTTTTTTTGTTTTTTTTTTGATTAGT‑AA‑AAAA‑TTg                                                                                                          >  1:883588/1‑140 (MQ=255)

                                   aTTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAA‑ACGCCTGGGAAACCGGGCGTTTTTTTGTTGTTTTTTATGTAAATACA‑AA‑AAAA‑TTTaaa                                                                                                       >  1:1134085/1‑140 (MQ=255)

                                   aTTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAA‑ACGCCTGGGAAACCGGGCGTGTTTTTTTTTTTTTTTTTTTTATTAAT‑AA‑AAAa‑ttttaa                                                                                                       >  1:4203892/1‑136 (MQ=255)

                                   aTTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAA‑ACGCCTGGGAAACCGGGCGTGTTTTTGTTGTTTTTTATGTAATTACT‑AA‑AAAA‑TTGaaa                                                                                                       >  2:3318103/1‑140 (MQ=255)

                                      ggCTTAAACGGCTTGCTGAATCGGTGCCCAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAA‑ACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACT‑AC‑AAAT‑TTGAAAAAt                                                                                                    <  2:228910/140‑1 (MQ=255)

                                       gCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAA‑ACGCCTGGGAAACCGGGCGTTTTTTTGTTAAATTTTATGTTATTAAT‑AC‑AAAT‑TTTaaaaaaa                                                                                                   >  2:187394/1‑138 (MQ=255)

                                        cTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAA‑ACGCCTGGGAAACCGGGCGTTTTTTTGTTTTTTTTTTTTTTATTAAT‑AC‑AAAA‑TTTataaaaaa                                                                                                  >  1:1857902/1‑137 (MQ=255)

                                        cTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAA‑ACGCCTGGGAAACCGGGCGTTTTTTGTTTCTTTTTTATTTAATTAAT‑AA‑AAAA‑TTGAAAAAT‑Ac                                                                                                 >  1:1095182/1‑140 (MQ=255)

                                        cTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAA‑ACGCCTGGGAAACCGGGCGTGTTTTTTTTGTTTTTTTTTTAATTAATAAA‑AAAT‑TTTaaaaaaa                                                                                                   >  1:1095163/1‑138 (MQ=255)

                                         tGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAA‑ACGCCTGGGAAACCGGGCGTGTTTTTGTTATTTTTTATGTTATTACG‑AC‑AAAT‑TTTGAAAAT‑aag                                                                                                >  2:1578662/1‑138 (MQ=255)

                                           aaaCGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAA‑ACGCCTGGGAAACCGGGCGTGTTTTTTGTGTTTTTTATTTAATTAAT‑AC‑AAAA‑TTTTAAAAT‑aacgg                                                                                              >  1:4650197/1‑136 (MQ=255)

                                             agggCTTGCTGAATCGGTGCAGAGCCGGGAAATTTTTAAAATTATACCTATTCAAACAAAGTAAAACAAAA‑ACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACT‑AC‑AAAT‑TTGAAAAAT‑ACGGGaa                                                                                            <  1:3078622/138‑1 (MQ=255)

                                               ggCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAA‑ACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACT‑AC‑AAAT‑TTGAAAAAT‑ACGGGaaaa                                                                                          <  2:2659387/140‑1 (MQ=255)

                                                 cTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAA‑ACGCCTGGGAAACCGGGCGTGGTTTTGTTGTTTTTTTTTTAATTAAT‑AA‑AAAA‑TTTAAAAAA‑AAGGGaaaaaa                                                                                        >  1:475529/1‑139 (MQ=255)

                                                    gcttattCGGTGCAGAGCGGGTAATTTTTTAAATTTATACCGATTGAAAAAAATTAAAACAAAC‑ACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACT‑AC‑AAAT‑TTGAAAAAT‑ACGGGAAAAACATc                                                                                     <  1:4320641/134‑1 (MQ=255)

                                                     cTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAA‑ACGCCTGGGAAACCGGGCGTGTTTTTTTTTTTTTTTTTGTTAATACT‑ACAAAAT‑TTAAAAAAA‑ACGGaaaaaaaaac                                                                                     >  2:2096635/1‑136 (MQ=255)

                                                         aTCGGTGCAGAGCGGGTAAATTTTTAAATTTATACCTATTTAAACAAAGTAAAAAAAAA‑ACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACT‑AC‑AAAT‑TTGAAAAAT‑ACGGGAAAAACATCCCAtt                                                                                <  2:642977/140‑1 (MQ=255)

                                                           cGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAA‑ACGCCTGGGAAACCGGGCGTGTTTTTGTTGTTTTTTATTTAATTACT‑AC‑AAAT‑TTTTAAAAT‑AAGGGAAAAACATACCATTTa                                                                              >  2:435542/1‑140 (MQ=255)

                                                             gTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAA‑ACGCCTGGGAAACCGGGCGTTTTTTTGTTGTTTTTTATGTAATTACT‑AC‑AAAT‑TTGAAAAAA‑ACGGGAAAAACAACACAATTaat                                                                            >  2:155477/1‑138 (MQ=255)

                                                             gTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAA‑ACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACT‑AC‑AAAT‑TTGAAAAAT‑ACGGGAAAAACATCCCATTTACt                                                                            >  2:4487926/1‑140 (MQ=255)

                                                                        ggAAATTTTTAAATTTATACCGATTGAAACAAAGTAATACAAAA‑ACGCCTGGGAAACCGGGCGTTGTTTTGTTGTTTTTTTTTTAATTTAT‑ACAAAAT‑TTTTAAAAA‑ACGGGAAAAACAACCC‑GTTAATTACAAAAAAtt                                                                 >  1:1835087/1‑140 (MQ=255)

                                                                        ggAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAA‑ACGCCTGGGAAACCGGGCGTTTTTTTTTTGTTTTTTATTTAATTAAT‑AA‑AAAA‑TTTAAAAAA‑AAGGGAAAAAAAAACCAATTAATTGCAAAATAtt                                                                 >  2:541282/1‑140 (MQ=255)

                                                                        ggAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAA‑ACGCCTGGGAAACCGGGCGTTTTTTTGTTGTTTTTTTTGTGATTTAT‑AC‑AAAT‑TTTTAAAAT‑TAGGGAAAAAAAACCCAATTTATTAAAAAATAtt                                                                 >  2:159719/1‑140 (MQ=255)

                                                                        ggAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAA‑ACGCCTGGGAAACCGGGCGTGTTTTTTTTGTTTTTTTTTTTATTTAA‑AA‑AAAT‑TTTTAAAATAAGGGGAAAAAAAACCCATTAAGTTCAaaaaatt                                                                  >  1:3231029/1‑137 (MQ=255)

                                                                        ggAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAA‑ACGCCTGGGAAACCGGGCGTGTTTTTGTTTTTTTTTATGTAATTAAT‑AC‑AAAT‑ATTTAAAAA‑AAGGGAAAAAAATACCCATTAATTACAAAATAtt                                                                 >  2:344269/1‑140 (MQ=255)

                                                                        ggAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAA‑ACGCCTGGGAAACCGGGCGTGTTTTTGTTTTTTTTTATGTAATTAAT‑AC‑AAAA‑TTGAAAAAA‑AAGGGAAAAACATCACATTTAATTACAAAAAAtt                                                                 >  2:3281123/1‑140 (MQ=255)

                                                                        ggAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAA‑ACGCCTGGGAAACCGGGCGTGGTTTTTTTGTTTTTTTTTTAAATAAT‑AA‑AAAA‑ATTAAAAAA‑AAGGGGAAAACAAACCAAATAATTACaaaaaaat                                                                 >  1:2028718/1‑136 (MQ=255)

                                                                             tttttAAATTTATACCGCTTGAAACAAAGTAAAACAAAA‑ACGCCTGGGAAACCGGGCGTTGTGTTGTTGTTTTTTATGTAATTAAT‑AA‑AAAT‑TTGATAAAT‑ACGGGAAATACATCCCATTTAGTTCAAAAATTGTTGATg                                                            >  2:2728496/1‑140 (MQ=255)

                                                                              ttttAAATTTATACCGATTGAAACAAATTAAAACAAAA‑ACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACT‑AC‑AAAT‑TTGAAAAAT‑ACGGGAAAAACATCCCATTTACTTCCAACATATTTGATgg                                                           <  2:2267693/140‑1 (MQ=255)

                                                                              ttttAAATTTATACCGATTGAAACAAAGTAAAACAAAA‑ACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACT‑AC‑AAAT‑TTGAAAAAT‑ACGGGACAAACATCCCATTTACTTCCAAAATATTTGATgg                                                           <  1:1945512/140‑1 (MQ=255)

                                                                               tttAAATTTATACCGATTGAAACAAAGTAAAACAAAA‑ACGCCTGGGAAACCGGGCGTGTTTTTGTTGTTTTTTATGTAAATACT‑AC‑AAAA‑TTGAAAAAT‑AAGGGAAAAACAAACCATATACTTCCAAAAAATTTGTTGGt                                                          >  2:4560767/1‑140 (MQ=255)

                                                                                        ataCCGATTCAAAAAAAGTAAAACAAAA‑ACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACT‑AC‑AAAT‑TTGAAAAAT‑ACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATg                                                 <  1:867029/140‑1 (MQ=255)

                                                                                             taTTCAAACAAATTAAAACAAAA‑ACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACT‑AC‑AAAT‑TTGAAAAAT‑ACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGt                                            <  2:1859773/139‑1 (MQ=255)

                                                                                             gATTTAAACAAAGTAAAAAAAAA‑ACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACT‑AC‑AAAT‑TTGAAAAAT‑ACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGt                                            <  2:1279061/140‑1 (MQ=255)

                                                                                             gATTCAAACAAAGTAAAACAAAC‑ACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACT‑AC‑AAAT‑TTGAAAAAT‑ACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGt                                            <  1:3318103/140‑1 (MQ=255)

                                                                                             caTTGAAACACAGTAAACCAACT‑ACGCCTGGGAAACCGGGCGTTTTTTTTTTCTTTTTTATGTAATTACT‑AC‑AAAT‑TTGAAAAAT‑ACGGGAAACACATCCAATTTACTTCCAAAATATTTGATGTTAATTTTATGTAGGt                                            <  1:3849088/139‑1 (MQ=255)

                                                                                              aTTGAAACAAAGTAAAACAAAA‑ACGCCTGGGAAACCGGGCGTTTTTTTGTTGTTTTTTTTGTTATTACT‑AC‑AAAT‑TTTAAAAAT‑AAGGGTAAAACATACCAATTAATTACAAAATATTTGATGGTAATTTTTtgtatgta                                           >  2:2000705/1‑140 (MQ=255)

                                                                                              aTTGAAACAAAGTAAAACAAAA‑ACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATTTAATTACT‑AC‑AAAT‑TTGAAAAAA‑ACGGGAAAAAAAACCCAGTTAATTCCAAAAAATTTGATGGTAATTTTAtgtatgta                                           >  2:409320/1‑140 (MQ=255)

                                                                                              aTTGAAACAAAGTAAAACAAAA‑ACGCCTGGGAAACCGGGCGTGTTTTTGTTTTTTTTTTTGTTATTTAT‑AA‑AAATATTTAAAAAA‑ATGGCCAAAAAAACCCATTAAGTACAAAAAAATTTTAT‑GTAATTTTAttgtggga                                           >  1:2860085/1‑133 (MQ=255)

                                                                                                tGAAACAAAGTAAAACAAAA‑ACGCCTGGGAAACCGGGCGTGGTTTTGTTGTTTTTTATTTAATTTAT‑AA‑AAAA‑TTTTAAAAT‑ACGGGAAAAAAATCCCATTTAATTACAAAATATTTGATTGTAAATTTATTTAGGTaaa                                         >  2:3970098/1‑140 (MQ=255)

                                                                                                  aaacaaacTAACACAAAA‑ACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACT‑CC‑AAAT‑TTGAAAAAT‑ACGGGAAAAACATCCCATTTACTGCCAAAATATTTGATGGTAATTTTATGTAGGTAAAAt                                       <  2:2950794/140‑1 (MQ=255)

                                                                                                  aaaaaaAGTAAAACAAAC‑ACGCCTGGGAAACCGGTCGTTTTTTTGTTCTTTTTTATGTAATTACT‑AC‑AAAT‑TTGAAAAAT‑ACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAAt                                       <  2:2787353/140‑1 (MQ=255)

                                                                                                  aaaCAAAGTAAAACAAAC‑ACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACT‑AC‑AAAT‑TTGAAAAAT‑ACGCGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAAt                                       <  1:4333734/140‑1 (MQ=255)

                                                                                                  aaaCAAAGTAAAACAAAA‑ACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACT‑AC‑AAAT‑TTGAAAAAT‑ACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAAt                                       <  2:883588/140‑1 (MQ=255)

                                                                                                  aaaCAAAGTAAAACAAAA‑ACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACT‑AC‑AAAT‑TTGAAAAAT‑ACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAAt                                       <  2:3361189/140‑1 (MQ=255)

                                                                                                         ctAACACAACA‑ACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACT‑AC‑AAAT‑TTGAAAAAT‑ACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTAtt                                <  2:3183286/139‑1 (MQ=255)

                                                                                                            caacacca‑aCGCCTGGGCAACCGGGCGTTTTTTTGTTCTTTTTTATGGACTTACT‑AC‑AAAT‑TTGAAACAT‑ACGGGAAAAACATCCCATTTACTTCCAAACTATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                             <  1:2095939/133‑1 (MQ=255)

                                                                                                            aaacaaac‑ACGCCTGGGAACCCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACT‑AC‑AAAT‑TTGAAAAAT‑ACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                             <  2:4203892/140‑1 (MQ=255)

                                                                                                            aaacaaac‑ACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACT‑AC‑AAAT‑TTGAAAAAT‑ACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                             <  2:1736751/140‑1 (MQ=255)

                                                                                                            aaaCAACC‑ACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACT‑AC‑AAAT‑TTGAAAAAT‑ACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                             <  2:4146427/140‑1 (MQ=255)

                                                                                                            aaaCAAAA‑ACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACT‑AC‑AAAT‑TTGAAAAAT‑ACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                             <  1:24433/140‑1 (MQ=255)

                                                                                                             aaCAAAACACGCCTGCGAACCCGCGCGTTTTTTTGTTCATGTTTATCTAATTACT‑AC‑AAAT‑TTGAAAAAT‑ACGCCAAAAACATCCCATGTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                             <  1:3986424/140‑1 (MQ=255)

                                                                                                             aaCAAAA‑ACGCCTGGGAAACCGGGCGTTTTTTTGTTGTTTTTTATTTAATTACT‑AA‑AAAT‑TTTAAAAAT‑ACGGGAAAAAAATCCCAATTACTTCCAAAATATTTTATGGTAATTTTATGTAGGTAAAAATTGTTTTTggg                            >  2:3215755/1‑139 (MQ=255)

                                                                                                                  aa‑aCGCCTGGGAAACCGGGCGTTTTTTTTTTGTTTTTTTTGTAATTACT‑AC‑AAAT‑TTGAAAATT‑ACGGGAAAAAAAACCCAGTTACTTGCAAAATATTTGATTGTTATTTTATGTATGTTAAATTTTTATTTTGCCGttg                       >  2:3489147/1‑139 (MQ=255)

                                                                                                                       gCCTGGGAAACCTGGCGTTTTTTTGTTCTTTTTTATGTAATTACT‑AC‑AAAT‑TTGAAAAAT‑ACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCagag                   <  1:2728496/140‑1 (MQ=255)

                                                                                                                       gCCTGGGAAACCGTGCGTTTTTTTGTTGTTTTTTATGTAATTACT‑AC‑TAAT‑TTGAAAAAT‑ACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTATGTAAATTTTGTATTTGGCCGTTCagag                   <  2:4331506/140‑1 (MQ=255)

                                                                                                                       gCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACT‑AC‑AAAT‑TTGAAAAAT‑ACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCagag                   <  2:2680140/140‑1 (MQ=255)

                                                                                                                       gCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACT‑AC‑AAAT‑TTGAAAAAT‑ACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCagag                   <  1:3425388/140‑1 (MQ=255)

                                                                                                                       gCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACT‑AC‑AAAT‑TTGAAAAAT‑ACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCagag                   <  1:2566303/140‑1 (MQ=255)

                                                                                                                       gCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACT‑AC‑AAAT‑TTGAAAAAT‑ACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCagag                   <  2:1095182/140‑1 (MQ=255)

                                                                                                                        ccTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACT‑AC‑AAAT‑TTGAAAAAT‑ACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAgagg                  <  1:1348141/140‑1 (MQ=255)

                                                                                                                             gAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACT‑AC‑AAAT‑TTGAAAAAT‑ACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTg             <  2:860910/140‑1 (MQ=255)

                                                                                                                                 ccGGGCGTTTTTTTGTTCTTTTTTATGTAATTACT‑AC‑AAAT‑TTGAAAAAT‑ACGGGAAACACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGaaaa         <  2:3053879/140‑1 (MQ=255)

                                                                                                                                  cGGGCGTTTTTTTGTTGTTTTTTATTTAAATAAT‑AC‑AAAA‑TTGAAAAAA‑AAGGGAAAAAAATACCAATTAATTCCAAAATATTTTATTGTAAATTTATTTATGTAAAATTTTTATTTTGCCGTTCAGAGGAGGTGAAAAg        >  2:2152018/1‑140 (MQ=255)

                                                                                                                                      cGTTTTTTTGTTCTTTTTTATGTAATTACT‑AC‑AAAT‑TTGAAAAAT‑ACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCggg    <  1:3552772/140‑1 (MQ=255)

                                                                                                                                      cGTTTTTTTGTTCTTTTTTATGTAATTACT‑AC‑AAAT‑TTGAAAAAT‑ACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCggg    <  2:511412/140‑1 (MQ=255)

ATACGAGCAACTGGAAAGCTCTAAAGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAA‑ACGCCTGGGAAACCGGGCGTTTTTTTGTTCTTTTTTATGTAATTACT‑AC‑AAAT‑TTGAAAAAT‑ACGGGAAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCGGGCA  >  NZ_CP034943/2991091‑2991365

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`