BRESEQ :: Evidence

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	2,991,236	0	C	G	37.9%	224.8 / 74.2	173	intergenic (+37/‑170)	gbsR/bslA	transcriptional regulator GbsR/biofilm‑surface layer protein BslA
Reads supporting (aligned to +/- strand): ref base C (10/85); new base G (57/1); total (87/86)
Fisher's exact test for biased strand distribution p-value = 2.59e-30
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 175 total aligned reads displayed.

AACTGGAAAGCTCTAAAGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTT‑CTTTTTTATGTAATTACT‑AC‑AAATTTGAAAAATACGGG‑AAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCGGGCAGGCCTGAAT  >  NZ_CP034943/2991099‑2991374

aaCTGGAAAGCTCTAAAGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTgtt‑gtt                                                                                                                                             >  2:1084494/1‑140 (MQ=255)

 aCTGGAAAGCTCTAAAGCTTACTATGATGGGCTGAAACGGCTTGCTGAATCGGTGCAGCGCGGGGAAATTTTTAAATTTATACCGATTGAACCAAAGTAAAAAAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTT‑Cttt                                                                                                                                            <  2:2453332/140‑1 (MQ=255)

  cTGGAAAGCTCTAAAGCTTACTATTATGGGCTCAACCGGCTTGCTCAATCGGTGCAGCGCGGGTAAATTTTTAAATTTATCCCGATTCAAACAAAGAAAAACAAACACGCCTGGGAAACCGGGCGTTTTTTTGTT‑Ctttt                                                                                                                                           <  1:2629712/140‑1 (MQ=255)

  cTGGAAAGCTCTAAAGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGTGTTTGTT‑Gtttt                                                                                                                                           >  1:642977/1‑140 (MQ=255)

   tGGAAAGATCTAAAGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGTTTTTGTT‑Cttttt                                                                                                                                          >  2:4159293/1‑140 (MQ=255)

          ctctAAAGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGTTTTTTTT‑Gttttttttttta                                                                                                                                   >  1:2787353/1‑134 (MQ=255)

          ctctAAAGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGTTTTTTTT‑CTTGTTTTTTTaa                                                                                                                                   >  2:4042906/1‑140 (MQ=255)

           tctAAAGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTT‑CTTTTTTATGTAAt                                                                                                                                  <  1:1976978/140‑1 (MQ=255)

             tAAAGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCTGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTT‑ATTTTTTATTTAATta                                                                                                                                >  2:2055659/1‑140 (MQ=255)

             tAAAGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTTTT‑GGTTTTTTTTTAATta                                                                                                                                >  1:272643/1‑140 (MQ=255)

             tAAAGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTTTT‑ATTTTTTATTTAATta                                                                                                                                >  2:1300097/1‑140 (MQ=255)

             tAAAGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTT‑ATTTTTTATGATATta                                                                                                                                >  2:3084294/1‑140 (MQ=255)

             tAAAGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGTTTTTTTT‑TTTTTTTTTGTAAttt                                                                                                                                >  2:3692776/1‑139 (MQ=255)

             tAAAGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGTTTTTGTT‑GTTTTTTATGTAATta                                                                                                                                >  2:4665366/1‑140 (MQ=255)

                   ttACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAATGTAAAACAAAAACGCCTGGGAAACCGGGCGTGGTTTTGTT‑TGTTTTTTTGTAATTACT‑AG‑aa                                                                                                                        >  1:4177440/1‑140 (MQ=255)

                    tACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAAAGCCTGGGAAACCGGGCGTTTTTTTTTT‑GTTTTTTATGTAATTAAA‑AC‑aaa                                                                                                                       >  2:1516405/1‑140 (MQ=255)

                     aCTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTTTT‑GTTTTTTATGTAAATACT‑AC‑AAAt                                                                                                                      >  2:386/1‑140 (MQ=255)

                     aCTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGTTTTTTTT‑GTTTTTTTTTTTATTTAT‑AC‑aaaa                                                                                                                      >  1:2471466/1‑139 (MQ=255)

                     aCTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGTTTTTTTT‑GTTTTTTATTTTATTAAT‑AA‑AAAt                                                                                                                      >  2:4158856/1‑140 (MQ=255)

                     aCTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGTTTTTGTT‑ATTTTTTATTTAATTAAT‑Aa‑aaaa                                                                                                                      >  1:1573621/1‑139 (MQ=255)

                     aCTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGGTGTTTTT‑GTTTTTTTTTTATTTAAT‑AA‑AAAt                                                                                                                      >  1:3214426/1‑140 (MQ=255)

                        ttGATTGGGTGAACCGGCTGGCTCAATCGGTGCCGAGCGGGGAATTTTTTAAATTTATACCGATTTAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTT‑CTTTTTTATGTAATTACT‑AC‑AAATTTg                                                                                                                   <  1:4664678/139‑1 (MQ=255)

                        aTGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAATCCGGGGGTTTGTTTGGT‑GTTGTTTTTGTTGGTTCT‑AC‑AAATTTg                                                                                                                   >  1:3706889/1‑140 (MQ=255)

                        aTGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGTTTTTTTT‑GTTTTTTTTTTGATTAGT‑AA‑AAAATTg                                                                                                                   >  1:883588/1‑140 (MQ=255)

                           aTTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTT‑GTTTTTTATGTAAATACA‑AA‑AAAATTTaaa                                                                                                                >  1:1134085/1‑140 (MQ=255)

                           aTTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGTTTTTGTT‑GTTTTTTATGTAATTACT‑AA‑AAAATTGaaa                                                                                                                >  2:3318103/1‑140 (MQ=255)

                            ttGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGGTTTTTGT‑GTTTTTTTTTTTATTTGT‑AA‑AAATTTTaaaa                                                                                                               >  1:257753/1‑140 (MQ=255)

                              ggCTTAAACGGCTTGCTGAATCGGTGCCCAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTT‑CTTTTTTATGTAATTACT‑AC‑AAATTTGAAAAAt                                                                                                             <  2:228910/140‑1 (MQ=255)

                                cTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTT‑TTTTTTTTTTTTATTAAT‑AC‑AAAATTTataaaaaa                                                                                                           >  1:1857902/1‑137 (MQ=255)

                                cTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGTTTTTTTT‑GTTTTTTTTTTAATTAATAAA‑AAATTTTaaaaaaa                                                                                                            >  1:1095163/1‑138 (MQ=255)

                                 tGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTGTTGTTGTTTTTTTTTGTAA‑TAAT‑AA‑AAATTTGaaaaaaaac                                                                                                          >  1:2160614/1‑136 (MQ=255)

                                 tGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGTTTTTGTT‑ATTTTTTATGTTATTACG‑AC‑AAATTTTGAAAATaag                                                                                                          >  2:1578662/1‑138 (MQ=255)

                                   aaaCGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGTTTTTTGT‑GTTTTTTATTTAATTAAT‑AC‑AAAATTTTAAAATaacgg                                                                                                        >  1:4650197/1‑136 (MQ=255)

                                     aCGGCTTGCTGAATCGGTGCAGAGCGGGGAAATATTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTGTTTGTTT‑GTTTTTTAAGTGAAAACA‑AC‑AAGTTTGATAAATACTGG‑aa                                                                                                     >  2:2495847/1‑140 (MQ=255)

                                       ggCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTT‑CTTTTTTATGTAATTACT‑AC‑AAATTTGAAAAATACGGG‑aaaa                                                                                                   <  2:2659387/140‑1 (MQ=255)

                                         cTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTTTT‑GTTTTTTATGTTATTAGA‑AA‑CAAATTGAAAAATACCGG‑AAAAAc                                                                                                 >  1:812638/1‑140 (MQ=255)

                                         cTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGGTTTTGTT‑GTTTTTTTTTTAATTAAT‑AA‑AAAATTTAAAAAAAAGGG‑aaaaaa                                                                                                 >  1:475529/1‑139 (MQ=255)

                                             cTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGTTTTTTTT‑TTTTTTTTTGTTAATACT‑ACAAAATTTAAAAAAAACGGa‑aaaaaaaac                                                                                              >  2:2096635/1‑136 (MQ=255)

                                                 aTCGGTGCAGAGCGGGTAAATTTTTAAATTTATACCTATTTAAACAAAGTAAAAAAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTT‑CTTTTTTATGTAATTACT‑AC‑AAATTTGAAAAATACGGG‑AAAAACATCCCAtt                                                                                         <  2:642977/140‑1 (MQ=255)

                                                 aTCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCTATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTT‑CTTTTTTATGTAATTACT‑AC‑AAATTTGAAAAATACGGG‑AAAAACATCCCAtt                                                                                         <  2:1617290/140‑1 (MQ=255)

                                                    ggTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTT‑CTTTTTTATGTAATTACT‑AC‑AAATTTGAAAAATACGGG‑AAAAACATCCCATTTAc                                                                                      <  2:606923/140‑1 (MQ=255)

                                                     gTGCAGAGCGGGGAAATTTTTAAGTTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGTTGTGTTT‑ATTTTTTATGTAAATAAA‑AA‑AAATTTGAAAAATACGGG‑TAAAAAATAACAATTACt                                                                                     >  2:2274091/1‑140 (MQ=255)

                                                     gTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTT‑GTTTTTTATGTAATTACT‑AC‑AAATTTGAAAAAAACGGG‑AAAAACAACACAATTaat                                                                                     >  2:155477/1‑138 (MQ=255)

                                                     gTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTT‑CTTTTTTATGTAATTACT‑AC‑AAATTTGAAAAATACGGG‑AAAAACATCCCATTTACt                                                                                     >  2:4487926/1‑140 (MQ=255)

                                                     gTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGGTTTTGTT‑TTTTTTTTTGTTATTAATAAA‑AAATTTAAAAAAAAAGGC‑AAAAAAAACCCagtaat                                                                                      >  1:2938461/1‑135 (MQ=255)

                                                           agCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGGGTTTTTT‑GTTTTTTATTTAATTAAT‑AC‑AAAATTTTAAAAAAAGGG‑AAAAAAATACCAGTTAATTACaaa                                                                               >  1:164065/1‑140 (MQ=255)

                                                             cGGGGAAATTTTTAAATTTATACCGATTGAAACAATGTAAAACAAATACGCCTGGGAAACCGGGCGTGGTTTTGGT‑GTGTGTTATGTACATAGT‑AC‑CAATTTGACACATACGGG‑AAAACCAGCCCAGTGACTTCCaaact                                                                             >  2:2956734/1‑138 (MQ=255)

                                                                ggAAATTTTTAAATTTATACCGATTGAAACAAAGTAATACAAAAACGCCTGGGAAACCGGGCGTTGTTTTGTT‑GTTTTTTTTTTAATTTAT‑ACAAAATTTTTAAAAAACGGG‑AAAAACAACCC‑GTTAATTACAAAAAAtt                                                                          >  1:1835087/1‑140 (MQ=255)

                                                                ggAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGTTTTTGTT‑TTTTTTTATGTAATTAAT‑AC‑AAATATTTAAAAAAAGGG‑AAAAAAATACCCATTAATTACAAAATAtt                                                                          >  2:344269/1‑140 (MQ=255)

                                                                ggAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGTTTTTGTT‑TTTTTTTATGTAATTAAT‑AC‑AAAATTGAAAAAAAAGGG‑AAAAACATCACATTTAATTACAAAAAAtt                                                                          >  2:3281123/1‑140 (MQ=255)

                                                                ggAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGTTTTTGTT‑GTTTTTTTTTTAATTAAT‑AC‑AAAATTTAAAAAAAAGGG‑AAAAAAAAACCAATTAGTTACAAAATAtt                                                                          >  1:2028467/1‑140 (MQ=255)

                                                                 taaaTTTTTAAATTTATACCGATTTAAACAAAGTAAACAAAACACGCCTGGGAAACCGGGCGTTTTTTTGTT‑CTTTTTTATGTAATTACT‑AC‑AAATTTGAAAAATACGGG‑AAAAACATCCCATTTACTTCCAAAATAttt                                                                         <  1:2096635/139‑1 (MQ=255)

                                                                     tttttAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTT‑CTTTTTTATGTAATTACT‑AC‑AAATTTGAAAAATACGGG‑AAAAACATCCCATTTACTTCCAAAATATTTGATg                                                                     <  2:1863606/140‑1 (MQ=255)

                                                                      ttttAAATTTATACCGATTTAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTT‑CTTTTTTATGTAATTACT‑AC‑AAATTTGAAAAATACGGG‑AAAAACATCCCATTTACTTCCAAAATATTTGATgg                                                                    <  1:3281123/140‑1 (MQ=255)

                                                                      ttttAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTT‑CTTTTTTATGTAATTACT‑AC‑AAATTTGAAAAATACGGG‑ACAAACATCCCATTTACTTCCAAAATATTTGATgg                                                                    <  1:1945512/140‑1 (MQ=255)

                                                                                ataCCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTT‑CTTTTTTATGTAATTACT‑AC‑AAATTTGAAAAATACGGG‑AAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATg                                                          <  2:2749362/140‑1 (MQ=255)

                                                                                     gATTTAAACAAAGTAAAAAAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTT‑CTTTTTTATGTAATTACT‑AC‑AAATTTGAAAAATACGGG‑AAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGt                                                     <  2:1279061/140‑1 (MQ=255)

                                                                                     gATTCAAACAAAGTAAAACAAACACGCCTGGGAAACCGGGCGTTTTTTTGTT‑CTTTTTTATGTAATTACT‑AC‑AAATTTGAAAAATACGGG‑AAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGt                                                     <  1:3318103/140‑1 (MQ=255)

                                                                                      aTTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTT‑GTTTTTTTTGTTATTACT‑AC‑AAATTTTAAAAATAAGGG‑TAAAACATACCAATTAATTACAAAATATTTGATGGTAATTTTTtgtatgta                                                    >  2:2000705/1‑140 (MQ=255)

                                                                                      aTTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTT‑CTTTTTTATTTAATTACT‑AC‑AAATTTGAAAAAAACGGG‑AAAAAAAACCCAGTTAATTCCAAAAAATTTGATGGTAATTTTAtgtatgta                                                    >  2:409320/1‑140 (MQ=255)

                                                                                      aTTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTGTTTTTTT‑GTTTTTTTTTTAAATAAT‑AA‑AAAAATTAAAAAAACGGG‑AAAAAAAAACCAATTAAATACAAAAAATTTTTAGGTAATTTTAtgtatgta                                                    >  2:4039830/1‑140 (MQ=255)

                                                                                      aTTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGTTTTTTTT‑GTTTTTTTTGTAAATAAT‑AC‑AAATTTTTAAAATAAGGG‑AAAAAAAAAACAATTAATTACAAAATATTTTATTGTAATTATGTGTatttt                                                    >  2:1374126/1‑136 (MQ=255)

                                                                                       ttGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGGT‑CTTTTTTATGTAATTACT‑AA‑AAATTTGAAAAATAAGGGTAAAAAAAACCCA‑TTAATTGCAAAATATTTTCTGGTAATTTTAATTAGGTaa                                                   >  2:4550720/1‑140 (MQ=255)

                                                                                        tGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTGGTTTTGTT‑GTTTTTTATTTAATTTAT‑AA‑AAAATTTTAAAATACGGG‑AAAAAAATCCCATTTAATTACAAAATATTTGATTGTAAATTTATTTAGGTaaa                                                  >  2:3970098/1‑140 (MQ=255)

                                                                                          aaacaaacTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTT‑CTTTTTTATGTAATTACT‑AC‑AAATTTGAAAAATACGGG‑AAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAAt                                                <  2:3706889/140‑1 (MQ=255)

                                                                                          aaaaaaATTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTT‑CTTTTTTATGTAATTACT‑AC‑AAATTTGAAAAATACGGG‑AAAAAAATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAAt                                                <  1:3970098/140‑1 (MQ=255)

                                                                                          aaaaaaAGTAAAACAAACACGCCTGGGAAACCGGTCGTTTTTTTGTT‑CTTTTTTATGTAATTACT‑AC‑AAATTTGAAAAATACGGG‑AAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAAt                                                <  2:2787353/140‑1 (MQ=255)

                                                                                          aaaCAAAGTAAAACAAACACGCCTGGGAAACCGGGCGTTTTTTTGTT‑CTTTTTTATGTAATTACT‑AC‑AAATTTGAAAAATACGGG‑AAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAAt                                                <  2:257753/140‑1 (MQ=255)

                                                                                          aaaCAAAGTAAAACAAACACGCCTGGGAAACCGGGCGTTTTTTTGTT‑CTTTTTTATGTAATTACT‑AC‑AAATTTGAAAAATACGCG‑AAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAAt                                                <  1:4333734/140‑1 (MQ=255)

                                                                                          aaaCAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTT‑CTTTTTTATGTAATTACT‑AC‑AAATTTGAAAAATACGGG‑AAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAAt                                                <  2:883588/140‑1 (MQ=255)

                                                                                          aaaCAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTT‑CTTTTTTATGTAATTACT‑AC‑AAATTTGAAAAATACGGG‑AAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAAt                                                <  2:3361189/140‑1 (MQ=255)

                                                                                                aGTAAAACAAAAACGCCTGGGACACCGGGCGTTTTTTTTGT‑GTTTTTTATGTAATTACG‑AA‑AAATTTTAAAAATACTGG‑AAAAAAAACCCAGTTACGTCCAAAATAGGTGATGGTAATTTTATGTAGGTAAAATTTGTAt                                          >  2:2195306/1‑140 (MQ=255)

                                                                                                 ctAACACAACAACGCCTGGGAAACCGGGCGTTTTTTTGTT‑CTTTTTTATGTAATTACT‑AC‑AAATTTGAAAAATACGGG‑AAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTAtt                                         <  2:3183286/139‑1 (MQ=255)

                                                                                                    aaacaaacACGCCTGGGAACCCGGGCGTTTTTTTGTT‑CTTTTTTATGTAATTACT‑AC‑AAATTTGAAAAATACGGG‑AAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                                      <  2:4203892/140‑1 (MQ=255)

                                                                                                    aaacaaacACGCCTGGGAAACCGGGCGTTTTTTTGTT‑CTTTTTTATGTAATTACT‑AC‑AAATTTGAAAAATACGGG‑AAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                                      <  2:1736751/140‑1 (MQ=255)

                                                                                                    aaacaaacACGCCTGGGAAACCGGGCGTTTTTTTGTT‑CTTTTTTATGTAATTACT‑AC‑AAATTTGAAAAATACGGG‑AAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                                      <  1:2832158/140‑1 (MQ=255)

                                                                                                    aaaCAACCACGCCTGGGAAACCGGGCGTTTTTTTGTT‑CTTTTTTATGTAATTACT‑AC‑AAATTTGAAAAATACGGG‑AAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                                      <  2:4146427/140‑1 (MQ=255)

                                                                                                    aaaCAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTT‑CTTTTTTATGTAATTACT‑AC‑AAATTTGAAAAATACGGG‑AAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                                      <  1:4487926/140‑1 (MQ=255)

                                                                                                    aaaCAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTT‑CTTTTTTATGTAATTACT‑AC‑AAATTTGAAAAATACGGG‑AAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                                      <  1:24433/140‑1 (MQ=255)

                                                                                                     aaCAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTT‑GTTTTTTATTTAATTACT‑AA‑AAATTTTAAAAATACGGG‑AAAAAAATCCCAATTACTTCCAAAATATTTTATGGTAATTTTATGTAGGTAAAAATTGTTTTTggg                                     >  2:3215755/1‑139 (MQ=255)

                                                                                                          aaaCGCCTGGGAAACCGGGCGTTTTTTTTTT‑GTTTTTTTTGTAATTACT‑AC‑AAATTTGAAAATTACGGG‑AAAAAAAACCCAGTTACTTGCAAAATATTTGATTGTTATTTTATGTATGTTAAATTTTTATTTTGCCGttg                                >  2:3489147/1‑139 (MQ=255)

                                                                                                          aaaCGCCTGGGAAACCGGGCGTGTTTTTGTT‑GTTTTTTATGTAATTAAT‑AA‑CAAATTGAAAAATAAGGG‑AAAAAAATAACAGTTTCTTCCAAAAAGTTTTATGGTAAGTTTATGTATGTAAAAGTTTGATTTTGCCggag                                >  2:3018318/1‑137 (MQ=255)

                                                                                                            aCGCCTGGGAAACCGGGCGTTTTTTTGTT‑CTTTTTTATGTAATTACG‑AC‑AAATTTGAAAAATACGGG‑AAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCag                              <  2:2860085/140‑1 (MQ=255)

                                                                                                              gCCTGGGAAACCGGGCGTTTTTTTGTT‑CTTTTTTATGTAATTACT‑AC‑AAATTTGAAAAATACGGG‑AAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCagag                            <  2:4352913/140‑1 (MQ=255)

                                                                                                              gCCTGGGAAACCGGGCGTTTTTTTGTT‑CTTTTTTATGTAATTACT‑AC‑AAATTTGAAAAATACGGG‑AAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCagag                            <  2:1095182/140‑1 (MQ=255)

                                                                                                              gCCTGGGAAACCGGGCGTTTTTTTGTT‑CTTTTTTATGTAATTACT‑AC‑AAATTTGAAAAATACGGG‑AAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCagag                            <  1:4283423/140‑1 (MQ=255)

                                                                                                              gCCTGGGAAACCGGGCGTTTTTTTGTT‑CTTTTTTATGTAATTACT‑AC‑AAATTTGAAAAATACGGG‑AAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCagag                            <  2:1857902/140‑1 (MQ=255)

                                                                                                              gCCTGGGAAACCGGGCGTTTTTTTGTT‑CTTTTTTATGTAATTACT‑AC‑AAATTTGAAAAATACGGG‑AAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCagag                            <  1:3425388/140‑1 (MQ=255)

                                                                                                              gCCTGGGAAACCGGGCGTTTTTTTGTT‑CTTTTTTATGTAATTACT‑AC‑AAATTTGAAAAATACGGG‑AAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCagag                            <  2:2680140/140‑1 (MQ=255)

                                                                                                               ccTGGGAAACCGGGCGTTTTTTTGTT‑CTTTTTTATGTAATTACT‑AC‑AAATTTGAAAAATACGGG‑AAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAgagg                           <  1:421641/140‑1 (MQ=255)

                                                                                                               ccTGGGAAACCGGGCGTTTTTTTGTT‑CTTTTTTATGTAATTACT‑AC‑AAATTTGAAAAATACGGG‑AAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAgagg                           <  1:2042555/140‑1 (MQ=255)

                                                                                                               ccTGGGAAACCGGGCGTTTTTTTGTT‑CTTTTTTATGTAATTACT‑AC‑AAATTTGAAAAATACGGG‑AAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAgagg                           <  1:1348141/140‑1 (MQ=255)

                                                                                                                    gAAACCGGGCGTTTTTTTGTT‑CTTTTTTATGTAATTACT‑AC‑AAATTTGAAAAATACGGG‑AAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTg                      <  2:860910/140‑1 (MQ=255)

                                                                                                                             cGTTTTTTTGTT‑CTTTTTTATGTAATTACT‑AC‑AAATTTGAAAAATACGGG‑AAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCggg             <  1:3552772/140‑1 (MQ=255)

                                                                                                                             cGTTTTTTTGTT‑CTTTTTTATGTAATTACT‑AC‑AAATTTGAAAAATACGGG‑AAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCggg             <  1:3838807/140‑1 (MQ=255)

                                                                                                                             cGTTTTTTTGTT‑CTTTTTTATGTAATTACT‑AC‑AAATTTGAAAAATACGGG‑AAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCggg             <  2:511412/140‑1 (MQ=255)

                                                                                                                             cGTATTTTTGTT‑CTTTTTTATGTAATTACT‑AC‑AAATTTGAAAAATACGCG‑AAAAACATCCCATTTACGTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCggg             <  1:4626028/140‑1 (MQ=255)

                                                                                                                                  ttttGTT‑CTTTTTTATGTAATTACT‑AC‑AAATTTGAAAAATACGGG‑AAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCGGGCAGGc        <  1:1084494/140‑1 (MQ=255)

                                                                                                                                    ttGTT‑CTTTTTTATGTAATTACT‑AC‑AAATTTGAAAAATACGGG‑AAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCGGGCAGGCCt      <  1:2755212/140‑1 (MQ=255)

                                                                                                                                     tGTT‑CTTTTTTATTTAATTAAT‑AC‑AAATTTGAAAAATAAGGG‑AAAAACATACCATTTACTTCCAAAATATTTTATTGTAATTTTATGTAGGTAAAATTTTTATTTTGCCGTTCAGAGGAGGTGAAAATCGGGCAGGCCTg     >  2:1136235/1‑140 (MQ=255)

AACTGGAAAGCTCTAAAGCTTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAAACGCCTGGGAAACCGGGCGTTTTTTTGTT‑CTTTTTTATGTAATTACT‑AC‑AAATTTGAAAAATACGGG‑AAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCGGGCAGGCCTGAAT  >  NZ_CP034943/2991099‑2991374

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`