BRESEQ :: Evidence

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	2,991,252	0	C	A	25.5%	295.6 / 27.5	162	intergenic (+53/‑154)	gbsR/bslA	transcriptional regulator GbsR/biofilm‑surface layer protein BslA
Reads supporting (aligned to +/- strand): ref base C (34/83); new base A (39/1); total (78/84)
Fisher's exact test for biased strand distribution p-value = 1.74e-15
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 164 total aligned reads displayed.

TTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAA‑ACGCCTGGGAAACCGGGCGTTTTTTTGTT‑CTTTTTTATGTAATTACT‑AC‑AAAT‑TTGAAAAATACGGG‑AAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCGGGCAGGCCTGAATATTTTTATAACTTTTT  >  NZ_CP034943/2991118‑2991390

 tACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAA‑ACGCCTGGGAAACCGGGCGTTTTTTTGTT‑GTTTTTTTTTTAATTAAT‑aa‑aat                                                                                                                                        >  2:4152612/1‑139 (MQ=255)

 tACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAA‑ACGCCTGGGAAACCGGGCGTGTTTTTTTT‑GTTTTTTATGTAATTAAT‑AC‑aaa                                                                                                                                        >  1:4555152/1‑140 (MQ=255)

 tACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAA‑ACGCCTGGGAAACCGGGCGTGTTTTTTTT‑ATTTTTTATTTAAATAAT‑Aa‑aaa                                                                                                                                        >  1:4352913/1‑140 (MQ=255)

 tACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAA‑ACGCCTGGGAAACCGGGCGTGTTTTTGTT‑GTTTTTTTTTTAATTAAA‑AC‑aaa                                                                                                                                        >  1:1766893/1‑140 (MQ=255)

 tACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAA‑AAGCCTGGGAAACCGGGCGTTTTTTTTTT‑GTTTTTTATGTAATTAAA‑AC‑aaa                                                                                                                                        >  2:1516405/1‑140 (MQ=255)

  aCTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAA‑ACGCCTGGGAAACCGGGCGTGTTTTTTTT‑GTTTTTTATTTTATTAAT‑AA‑AAAt                                                                                                                                       >  2:4158856/1‑140 (MQ=255)

  aCTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAA‑ACGCCTGGGAAACCGGGCGTGGTGTTTTT‑GTTTTTTTTTTATTTAAT‑AA‑AAAt                                                                                                                                       >  1:3214426/1‑140 (MQ=255)

     aTGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAA‑ACGCCTGGGAATCCGGGGGTTTGTTTGGT‑GTTGTTTTTGTTGGTTCT‑AC‑AAAT‑TTg                                                                                                                                   >  1:3706889/1‑140 (MQ=255)

     aTGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAA‑ACGCCTGGGAAACCGGGCGTGTTTTTTTT‑GTTTTTTTTTTGATTAGT‑AA‑AAAA‑TTg                                                                                                                                   >  1:883588/1‑140 (MQ=255)

        aTTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAA‑ACGCCTGGGAAACCGGGCGTTTTTTTGTT‑GTTTTTTATGTAAATACA‑AA‑AAAA‑TTTaaa                                                                                                                                >  1:1134085/1‑140 (MQ=255)

        aTTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAA‑ACGCCTGGGAAACCGGGCGTGTTTTTTTT‑TTTTTTTTTTTTATTAAT‑AA‑AAAa‑ttttaa                                                                                                                                >  1:4203892/1‑136 (MQ=255)

        aTTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAA‑ACGCCTGGGAAACCGGGCGTGTTTTTGTT‑AATTTTTATGTAATTACT‑AC‑ACAA‑TTGtaa                                                                                                                                >  2:1199559/1‑140 (MQ=255)

         ttGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAA‑ACGCCTGGGAAACCGGGCGTGGTTTTTGT‑GTTTTTTTTTTTATTTGT‑AA‑AAAT‑TTTaaaa                                                                                                                               >  1:257753/1‑140 (MQ=255)

           ggCTTAAACGGCTTGCTGAATCGGTGCCCAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAA‑ACGCCTGGGAAACCGGGCGTTTTTTTGTT‑CTTTTTTATGTAATTACT‑AC‑AAAT‑TTGAAAAAt                                                                                                                             <  2:228910/140‑1 (MQ=255)

            gCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGTAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAA‑ACGCCTGGGAAACCGGGCGTTTTTTTGTT‑CTTTTTTATGTAATTACT‑AC‑AAAT‑TTGAAAAATa                                                                                                                            <  1:4663924/140‑1 (MQ=255)

            gCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAA‑ACGCCTGGGAAACCGGGCGTTTTTTTGTT‑AAATTTTATGTTATTAAT‑AC‑AAAT‑TTTaaaaaaa                                                                                                                            >  2:187394/1‑138 (MQ=255)

             cTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAA‑ACGCCTGGGAAACCGGGCGTTTTTTTGTT‑GTTTTTTATTTACTTACT‑AA‑AAAT‑TTTTAAAATAc                                                                                                                           >  2:3852927/1‑140 (MQ=255)

             cTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAA‑ACGCCTGGGAAACCGGGCGTGTTTTTTTT‑GTTTTTTTTTTAATTAATAAA‑AAAT‑TTTaaaaaaa                                                                                                                            >  1:1095163/1‑138 (MQ=255)

              tGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAA‑ACGCCTGGGAAACCGGGCGTTTTGTTGTTGTTTTTTTTTGTAA‑TAAT‑AA‑AAAT‑TTGaaaaaaaac                                                                                                                          >  1:2160614/1‑136 (MQ=255)

              tGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAA‑ACGCCTGGGAAACCGGGCGTGTTTTTGTT‑ATTTTTTATGTTATTACG‑AC‑AAAT‑TTTGAAAATaag                                                                                                                          >  2:1578662/1‑138 (MQ=255)

                aaaCGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAA‑ACGCCTGGGAAACCGGGCGTGTTTTTTGT‑GTTTTTTATTTAATTAAT‑AC‑AAAA‑TTTTAAAATaacgg                                                                                                                        >  1:4650197/1‑136 (MQ=255)

                  agggCTTGCTGAATCGGTGCAGAGCCGGGAAATTTTTAAAATTATACCTATTCAAACAAAGTAAAACAAAA‑ACGCCTGGGAAACCGGGCGTTTTTTTGTT‑CTTTTTTATGTAATTACT‑AC‑AAAT‑TTGAAAAATACGGG‑aa                                                                                                                     <  1:3078622/138‑1 (MQ=255)

                  aCGGCTTGCTGAATCGGTGCAGAGCGGGGAAATATTTAAATTTATACCGATTGAAACAAAGTAAAACAAAA‑ACGCCTGGGAAACCGGGCGTTGTTTGTTT‑GTTTTTTAAGTGAAAACA‑AC‑AAGT‑TTGATAAATACTGG‑aa                                                                                                                     >  2:2495847/1‑140 (MQ=255)

                    ggCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAA‑ACGCCTGGGAAACCGGGCGTTTTTTTGTT‑CTTTTTTATGTAATTACT‑AC‑AAAT‑TTGAAAAATACGGG‑aaaa                                                                                                                   <  2:2659387/140‑1 (MQ=255)

                      cTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAA‑ACGCCTGGGAAACCGGGCGTTTTTTTTTT‑GTTTTTTATGTTATTAGA‑AA‑CAAA‑TTGAAAAATACCGG‑AAAAAc                                                                                                                 >  1:812638/1‑140 (MQ=255)

                         gcttattCGGTGCAGAGCGGGTAATTTTTTAAATTTATACCGATTGAAAAAAATTAAAACAAAC‑ACGCCTGGGAAACCGGGCGTTTTTTTGTT‑CTTTTTTATGTAATTACT‑AC‑AAAT‑TTGAAAAATACGGG‑AAAAACATc                                                                                                              <  1:4320641/134‑1 (MQ=255)

                         gCTGACTCGGCGCCGCGCGGGGAAATTTTTAAATTTATACCGATTCAAACAACGTAAAACAAAC‑ACGCCTGGGAAACCGGGCGTTTTTTTGTT‑CTTTTTTATGTAATTACT‑AC‑AAAT‑TTGAAAAATACGGG‑AAAAACATc                                                                                                              <  1:4665366/140‑1 (MQ=255)

                          cTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAA‑ACGCCTGGGAAACCGGGCGTGTTTTTTTT‑TTTTTTTTTGTTAATACT‑ACAAAAT‑TTAAAAAAAACGGa‑aaaaaaaac                                                                                                              >  2:2096635/1‑136 (MQ=255)

                              atggttGCAGAGCGGTGAAATTTTTTAATTTATACAGATTGAAAAAAAGTAAAACAAAA‑ACGCCTGGGAAACCGGGCGTTTTTTTGTT‑CTTTTTTATGTAATTACT‑AC‑AAAT‑TTGAAAAATACGGG‑AAAAACATCCCAtt                                                                                                         <  2:3486694/135‑1 (MQ=255)

                              aTCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCTATTGAAACAAAGTAAAACAAAA‑ACGCCTGGGAAACCGGGCGTTTTTTTGTT‑CTTTTTTATGTAATTACT‑AC‑AAAT‑TTGAAAAATACGGG‑AAAAACATCCCAtt                                                                                                         <  2:1617290/140‑1 (MQ=255)

                                cGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAA‑ACGCCTGGGAAACCGGGCGTGTTTTTGTT‑GTTTTTTATTTAATTACT‑AC‑AAAT‑TTTTAAAATAAGGG‑AAAAACATACCATTTa                                                                                                       >  2:435542/1‑140 (MQ=255)

                                 ggTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAA‑ACGCCTGGGAAACCGGGCGTTTTTTTGTT‑CTTTTTTATGTAATTACT‑AC‑AAAT‑TTGAAAAATACGGG‑AAAAACATCCCATTTAc                                                                                                      <  2:606923/140‑1 (MQ=255)

                                  gTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAA‑ACGCCTGGGAAACCGGGCGTGGTTTTGTT‑TTTTTTTTTGTTATTAATAAA‑AAAT‑TTAAAAAAAAAGGC‑AAAAAAAACCCagtaat                                                                                                      >  1:2938461/1‑135 (MQ=255)

                                        agCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAA‑ACGCCTGGGAAACCGGGCGTGGGTTTTTT‑GTTTTTTATTTAATTAAT‑AC‑AAAA‑TTTTAAAAAAAGGG‑AAAAAAATACCAGTTAATTACaaa                                                                                               >  1:164065/1‑140 (MQ=255)

                                          cGGGGAAATTTTTAAATTTATACCGATTGAAACAATGTAAAACAAAT‑ACGCCTGGGAAACCGGGCGTGGTTTTGGT‑GTGTGTTATGTACATAGT‑AC‑CAAT‑TTGACACATACGGG‑AAAACCAGCCCAGTGACTTCCaaact                                                                                             >  2:2956734/1‑138 (MQ=255)

                                             ggAAATTTTTAAATTTATACCGATTGAAACAAAGTAATACAAAA‑ACGCCTGGGAAACCGGGCGTTGTTTGGTT‑TTTTTTTTTGTAATTTCG‑AA‑AAAG‑TTTTTAAAAAAGGG‑CAAAACATCCCCTGTTATTGCAACATAtt                                                                                          >  1:1916522/1‑140 (MQ=255)

                                             ggAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAA‑ACGCCTGGGAAACCGGGCGTTTTTTTGTT‑GTTTTTTTTGTAATTACT‑AC‑AAAT‑TTTAAAAAAAAGGG‑AAAAACATACCATTTACTTCCAAAatatg                                                                                          >  2:2664118/1‑139 (MQ=255)

                                             ggAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAA‑ACGCCTGGGAAACCGGGCGTTTTTTTGTT‑GTTTTTTATTTAATTAGT‑AA‑AAAT‑TTGAAAAATAAGGG‑AAAAAAATCCCATTTACTTCCAAAATAtt                                                                                          >  2:1286928/1‑140 (MQ=255)

                                             ggAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAA‑ACGCCTGGGAAACCGGGCGTGTTTTTGTT‑TTTTTTTATGTAATTAAT‑AC‑AAAA‑TTGAAAAAAAAGGG‑AAAAACATCACATTTAATTACAAAAAAtt                                                                                          >  2:3281123/1‑140 (MQ=255)

                                             ggAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAA‑ACGCCTGGGAAACCGGGCGTGGTTTTTTT‑GTTTTTTTTTTAAATAAT‑AA‑AAAA‑ATTAAAAAAAAGGG‑GAAAACAAACCAAATAATTACaaaaaaat                                                                                          >  1:2028718/1‑136 (MQ=255)

                                              taaaTTTTTAAATTTATACCGATTTAAACAAAGTAAACAAAAC‑ACGCCTGGGAAACCGGGCGTTTTTTTGTT‑CTTTTTTATGTAATTACT‑AC‑AAAT‑TTGAAAAATACGGG‑AAAAACATCCCATTTACTTCCAAAATAttt                                                                                         <  1:2096635/139‑1 (MQ=255)

                                              gAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAAAAAAA‑ACGCCTGGGAAACCGGGCGTTTTTTTGTT‑CTTTTTTATGTAATTACT‑AC‑AAAT‑TTGAAAAATACGGG‑AAAAACATCCCATTTACTTCCAAAATAttt                                                                                         <  1:1355023/140‑1 (MQ=255)

                                                  tttttAAATTTATACCTATTGAAACAAAGAAAAACAAAC‑ACGCCTGGGAAACCGGGCGTTTTTTTGTT‑CTTTTTTATGTAATTACT‑AC‑AAAT‑TTGAAAAATACGGG‑AAAAACATCCCATTTACTTCCAAAATATTTGATg                                                                                     <  1:344269/140‑1 (MQ=255)

                                                  tttttAAATTTATACCGCTTGAAACAAAGTAAAACAAAA‑ACGCCTGGGAAACCGGGCGTTGTGTTGTT‑GTTTTTTATGTAATTAAT‑AA‑AAAT‑TTGATAAATACGGG‑AAATACATCCCATTTAGTTCAAAAATTGTTGATg                                                                                     >  2:2728496/1‑140 (MQ=255)

                                                  tttttAAATTTATACCGATTGAAACAAAGTAAAACAAAA‑ACGCCTGGGAAACCGGGCGTTTTTTTGTT‑CTTTTTTATGTAATTACT‑AC‑AAAT‑TTGAAAAATACGGG‑AAAAACATCCCATTTACTTCCAAAATATTTGATg                                                                                     <  2:1863606/140‑1 (MQ=255)

                                                   ttttAAATTTATACCTATTTAAAAAAATTAAAACAAAA‑ACGCCTGGGAAACCGGGCGTTTTTTTGTT‑CTTTTTTATGTAATTACT‑AC‑AAAT‑TTGAAAAATACGGG‑AAAAACATCCCATTTACTTCCAAAATATTTGATgg                                                                                    <  2:4127761/140‑1 (MQ=255)

                                                   ttttAAATTTATACCGATTTAAACAAAGTAAAACAAAA‑ACGCCTGGGAAACCGGGCGTTTTTTTGTT‑CTTTTTTATGTAATTACT‑AC‑AAAT‑TTGAAAAATACGGG‑AAAAACATCCCATTTACTTCCAAAATATTTGATgg                                                                                    <  1:3281123/140‑1 (MQ=255)

                                                   ttttAAATTTATACCGATTGAAACAAATTAAAACAAAA‑ACGCCTGGGAAACCGGGCGTTTTTTTGTT‑CTTTTTTATGTAATTACT‑AC‑AAAT‑TTGAAAAATACGGG‑AAAAACATCCCATTTACTTCCAACATATTTGATgg                                                                                    <  2:2267693/140‑1 (MQ=255)

                                                             ataCCGATTGAAACAAAGTAAAACAAAA‑ACGCCTGGGAAACCGGGCGTTTTTTTGTT‑GTTTTTTATGTAATTACT‑AA‑AAAT‑TTGTAAAATACGGG‑TAAAACATCCCATTTACTTGCAAAATATTTGATTGTAATTTTATg                                                                          >  2:3425388/1‑140 (MQ=255)

                                                                  gATTCAAACAAAGTAAAACAAAC‑ACGCCTGGGAAACCGGGCGTTTTTTTGTT‑CTTTTTTATGTAATTACT‑AC‑AAAT‑TTGAAAAATACGGG‑AAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGt                                                                     <  1:3318103/140‑1 (MQ=255)

                                                                  cactcaaacaaacAAAACCAAAC‑ACGCCTGGGAAACCGGGCGTTTTTTTGTT‑CTTTTTTATGTAATTACT‑AC‑AAAT‑TTGAAAAATACGGG‑AAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGt                                                                     <  1:3692776/135‑1 (MQ=255)

                                                                   aTTGAAACAAAGTAAAACAAAA‑ACGCCTGGGAAACCGGGCGTTTTTTTGTT‑CTTTTTTATTTAATTACT‑AC‑AAAT‑TTGAAAAAAACGGG‑AAAAAAAACCCAGTTAATTCCAAAAAATTTGATGGTAATTTTAtgtatgta                                                                    >  2:409320/1‑140 (MQ=255)

                                                                   aTTGAAACAAAGTAAAACAAAA‑ACGCCTGGGAAACCGGGCGTTGTTTTTTT‑GTTTTTTTTTTAAATAAT‑AA‑AAAA‑ATTAAAAAAACGGG‑AAAAAAAAACCAATTAAATACAAAAAATTTTTAGGTAATTTTAtgtatgta                                                                    >  2:4039830/1‑140 (MQ=255)

                                                                   aTTGAAACAAAGTAAAACAAAA‑ACGCCTGGGAAACCGGGCGTGTTTTTGTT‑TTTTTTTTTGTTATTTAT‑AA‑AAATATTTAAAAAAATGGC‑CAAAAAAACCCATTAAGTACAAAAAAATTTTAT‑GTAATTTTAttgtggga                                                                    >  1:2860085/1‑133 (MQ=255)

                                                                    ttGAAACAAAGTAAAACAAAA‑ACGCCTGGGAAACCGGGCGTTTTTTTGTT‑CTTTTTTAATTAATTACT‑AA‑CAAT‑TTGAAAAATACGGG‑AAAAACATAACAATTAATTACAAAAAATTTGATGGTAAATTTATggatgtta                                                                   >  2:693382/1‑133 (MQ=255)

                                                                    ttGAAACAAAGTAAAACAAAA‑ACGCCTGGGAAACCGGGCGTTTTTTTGGT‑CTTTTTTATGTAATTACT‑AA‑AAAT‑TTGAAAAATAAGGGTAAAAAAAACCCA‑TTAATTGCAAAATATTTTCTGGTAATTTTAATTAGGTaa                                                                   >  2:4550720/1‑140 (MQ=255)

                                                                     tGAAACAAAGTAAAACAAAA‑ACGCCTGGGAAACCGGGCGTGGTTTTGTT‑GTTTTTTATTTAATTTAT‑AA‑AAAA‑TTTTAAAATACGGG‑AAAAAAATCCCATTTAATTACAAAATATTTGATTGTAAATTTATTTAGGTaaa                                                                  >  2:3970098/1‑140 (MQ=255)

                                                                       acaCAAAGTAAAACAAAA‑CCGCCGGGGAAACCGGGCGTTTTTTTGTT‑CTTTTTTATTTAATTAAT‑AC‑AAAT‑TTGAAAAATACGGG‑AAAAACATCCCATTTACTTCCCAAATATTTGATGGTAATTTTATGTAGGTAAAAt                                                                <  1:1147651/138‑1 (MQ=255)

                                                                       aaaaaaATTAAAACAAAA‑ACGCCTGGGAAACCGGGCGTTTTTTTGTT‑CTTTTTTATGTAATTACT‑AC‑AAAT‑TTGAAAAATACGGG‑AAAAAAATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAAt                                                                <  1:3970098/140‑1 (MQ=255)

                                                                       aaaaaaAGTAAAACAAAC‑ACGCCTGGGAAACCGGTCGTTTTTTTGTT‑CTTTTTTATGTAATTACT‑AC‑AAAT‑TTGAAAAATACGGG‑AAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAAt                                                                <  2:2787353/140‑1 (MQ=255)

                                                                       aaaCAAAGTAAAACAAAA‑ACGCCTGGGAAACCGGGCGTTTTTTTGTT‑CTTTTTTATGTAATTACT‑AC‑AAAT‑TTGAAAAATACGGG‑AAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAAt                                                                <  2:3361189/140‑1 (MQ=255)

                                                                       aaaCAAAGTAAAACAAAA‑ACGCCTGGGAAACCGGGCGTTTTTTTGTT‑CTTTTTTATGTAATTACT‑AC‑AAAT‑TTGAAAAATACGGG‑AAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAAt                                                                <  2:883588/140‑1 (MQ=255)

                                                                             aGTAAAACAAAA‑ACGCCTGGGACACCGGGCGTTTTTTTTGT‑GTTTTTTATGTAATTACG‑AA‑AAAT‑TTTAAAAATACTGG‑AAAAAAAACCCAGTTACGTCCAAAATAGGTGATGGTAATTTTATGTAGGTAAAATTTGTAt                                                          >  2:2195306/1‑140 (MQ=255)

                                                                              ctAACACAACA‑ACGCCTGGGAAACCGGGCGTTTTTTTGTT‑CTTTTTTATGTAATTACT‑AC‑AAAT‑TTGAAAAATACGGG‑AAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTAtt                                                         <  2:3183286/139‑1 (MQ=255)

                                                                                 aaacaaac‑CCGCCTGGGAAACCGGGCGTTTTTTTGTT‑CTTTTTTATGTAATTACT‑AC‑AAAT‑TTGAAAAATACGGG‑AAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                                                      <  1:2776452/140‑1 (MQ=255)

                                                                                 aaacaaac‑ACGCCTGGGAACCCGGGCGTTTTTTTGTT‑CTTTTTTATGTAATTACT‑AC‑AAAT‑TTGAAAAATACGGG‑AAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                                                      <  2:4203892/140‑1 (MQ=255)

                                                                                 aaacaaac‑ACGCCTGGGAAACCGGGCGTTTTTTTGTT‑CTTTTTTATGTAATTACT‑AC‑AAAT‑TTGAAAAATACGGG‑AAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                                                      <  2:1736751/140‑1 (MQ=255)

                                                                                 aaaaaaaa‑CCGCCTGGGAAACCGGGCGTTTTTTTGTT‑CTTTTTTATGTAATTACT‑AC‑AAAT‑TTGAAAAATACGGG‑AAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                                                      <  2:3678644/140‑1 (MQ=255)

                                                                                 aaaCAAAA‑ACGCCTGGGAAACCGGGCGTTTTTTTGTT‑CTTTTTTATGTAATTACT‑AC‑AAAT‑TTGAAAAATACGGG‑AAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                                                      <  2:2396846/140‑1 (MQ=255)

                                                                                 aaaCAAAA‑ACGCCTGGGAAACCGGGCGTTTTTTTGTT‑CTTTTTTATGTAATTACT‑AC‑AAAT‑TTGAAAAATACGGG‑AAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                                                      <  1:4487926/140‑1 (MQ=255)

                                                                                 aaaCAAAA‑ACGCCTGGGAAACCGGGCGTTTTTTTGTT‑CTTTTTTATGTAATTACT‑AC‑AAAT‑TTGAAAAATACGGG‑AAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                                                      <  1:24433/140‑1 (MQ=255)

                                                                                 aaaCAAAA‑ACGCCTGGGAAACCGGGCGTTTTTTTGTT‑CTTTTTTATGTAATTACT‑AC‑AAAT‑TTGAAAAATACGGG‑AAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                                                      <  2:1343151/140‑1 (MQ=255)

                                                                                  aaCAAAACACGCCTGCGAACCCGCGCGTTTTTTTGTT‑CATGTTTATCTAATTACT‑AC‑AAAT‑TTGAAAAATACGCC‑AAAAACATCCCATGTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTgg                                                      <  1:3986424/140‑1 (MQ=255)

                                                                                  aaCAAAA‑ACGCCTGGGAAACCGGGCGTTTTTTTGTT‑GTTTTTTATTTAATTACT‑AA‑AAAT‑TTTAAAAATACGGG‑AAAAAAATCCCAATTACTTCCAAAATATTTTATGGTAATTTTATGTAGGTAAAAATTGTTTTTggg                                                     >  2:3215755/1‑139 (MQ=255)

                                                                                  aaCAAAA‑ACGCCTGGGAAACCGGGCGTGTTTTTTTT‑GTTTTTTTTTTAAATAAT‑AA‑AAAA‑TTTAAAAATAAGGG‑CAAAAAATACCATTTACATCCCAAAAAATTTATGGTAATTTTTTGTTGGTAAAATTTTTAttttgg                                                     >  2:3959745/1‑137 (MQ=255)

                                                                                       aa‑aCGCCTGGGAAACCGGGCGTTTTTTTGTT‑CTTTTTTATGTAATTACT‑AC‑AAAT‑TTGAAAAATACGGG‑AAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTc                                                <  2:1547870/140‑1 (MQ=255)

                                                                                          ccGCCTGGGAAACCGGGCGTTATTTTGTT‑CTTTTTTATGTAATTACT‑AC‑AAAT‑TTGAAAAATACGGG‑AAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCag                                              <  2:4668453/139‑1 (MQ=255)

                                                                                          aCGCCTGGGAAACCGGGCGTTTTTTTGTT‑CTTTTTTATGTAATTACG‑AC‑AAAT‑TTGAAAAATACGGG‑AAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCag                                              <  2:2860085/140‑1 (MQ=255)

                                                                                            gCCTGGGAAACCGGGCGTTTTTTTGTT‑CTTTTTTATGTAATTACT‑AC‑AAAT‑TTGAAAAATACGGG‑AAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCagag                                            <  2:4352913/140‑1 (MQ=255)

                                                                                            gCCTGGGAAACCGGGCGTTTTTTTGTT‑CTTTTTTATGTAATTACT‑AC‑AAAT‑TTGAAAAATACGGG‑AAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCagag                                            <  1:487799/140‑1 (MQ=255)

                                                                                            gCCTGGGAAACCGGGCGTTTTTTTGTT‑CTTTTTTATGTAATTACT‑AC‑AAAT‑TTGAAAAATACGGG‑AAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCagag                                            <  2:1095163/140‑1 (MQ=255)

                                                                                            gCCTGGGAAACCGGGCGTTTTTTTGTT‑CTTTTTTATGTAATTACT‑AC‑AAAT‑TTGAAAAATACGGG‑AAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCagag                                            <  2:1095182/140‑1 (MQ=255)

                                                                                            gCCTGGGAAACCGGGCGTTTTTTTGTT‑CTTTTTTATGTAATTACT‑AC‑AAAT‑TTGAAAAATACGGG‑AAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCagag                                            <  1:4283423/140‑1 (MQ=255)

                                                                                            gCCTGGGAAACCGGGCGTTTTTTTGTT‑CTTTTTTATGTAATTACT‑AC‑AAAT‑TTGAAAAATACGGG‑AAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCagag                                            <  1:2566303/140‑1 (MQ=255)

                                                                                            gCCTGGGAAACCGGGCGTTTTTTTGTT‑CTTTTTTATGTAATTACT‑AC‑AAAT‑TTGAAAAATACGGG‑AAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCagag                                            <  2:1857902/140‑1 (MQ=255)

                                                                                             ccTGGGAAACCGGGCGTTTTTTTGTT‑CTTTTTTATGTAATTACT‑AC‑AAAT‑TTGAAAAATACGGG‑AAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAgagg                                           <  1:2042555/140‑1 (MQ=255)

                                                                                                  gAAACCGGGCGTTTTTTTGTT‑CTTTTTTATGTAATTACT‑AC‑AAAT‑TTGAAAAATACGGG‑AAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTg                                      <  2:860910/140‑1 (MQ=255)

                                                                                                      ccGGGCGTTTTTTTGTT‑CTTTTTTATGTAATTACT‑AC‑AAAT‑TTGAAAAATACGGG‑AAACACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGaaaa                                  <  2:3053879/140‑1 (MQ=255)

                                                                                                      ccGGGCGTTTTTTTGTT‑CTTTTTTATGTAATTACT‑AC‑AAAT‑TTGAAAAATACGGG‑AAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGaaaa                                  <  1:1844539/140‑1 (MQ=255)

                                                                                                           cGTTTTTTTGTT‑CTTTTTTATGTAATTACT‑AC‑AAAT‑TTGAAAAATACGGG‑AAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCggg                             <  2:511412/140‑1 (MQ=255)

                                                                                                           cGTTTTTTTGTT‑CTTTTTTATGTAATTACT‑AC‑AAAT‑TTGAAAAATACGGG‑AAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCggg                             <  1:3838807/140‑1 (MQ=255)

                                                                                                             tttttttGTT‑CTTTTTTATGTAATTACT‑AC‑AAAT‑TTGAAAAATACGGG‑AAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCGGGCa                           <  1:2000705/140‑1 (MQ=255)

                                                                                                                ttttGTT‑CTTTTTTATGTAATTACT‑AC‑AAAT‑TTGAAAAATACGGG‑AAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCGGGCAGGc                        <  1:1084494/140‑1 (MQ=255)

                                                                                                                  ttGTT‑CTTTTTTATGTAATTACT‑AC‑AAAT‑TTGAAAAATACGGG‑AAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCGGGCAGGCCt                      <  1:2755212/140‑1 (MQ=255)

                                                                                                                      t‑CTTTTTTATGTAATTACT‑AC‑AAAT‑TTGAAAAATACGGG‑AAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCGGGCAGGCCTGAat                  <  1:2664118/140‑1 (MQ=255)

                                                                                                                      t‑CTTTTTTATGTAATTACT‑AC‑AAAT‑TTGAAAAATACGGG‑AAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCGGGCAGGCCTGAat                  <  2:3282313/140‑1 (MQ=255)

                                                                                                                                tGTAATTACT‑AC‑AAAT‑TTGAAAAATACGGG‑AAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCTTTCAGAGGAGGTGAAAATCGGGCAGGCCTGAATATTTTtata         >  1:3367427/1‑140 (MQ=255)

                                                                                                                                tGTAATTACT‑AC‑AAAT‑TTGAAAAATACGGG‑AAAAACATCCCATTTACTTCCAAAATATGGGATGGTAATTTTATGTAGTTAAAATTTGTATTTGGCCGTTCAGAGGAGGGGAAATGCGGGCCGGCGTGACTATTTTtata         >  1:3362416/1‑140 (MQ=255)

                                                                                                                                     ttACT‑AC‑AAAT‑TTGAAAAATACGGG‑AAAAACATCCCATTTAGTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCGGGCAGGCGTGAATATTTTTATTAGttt    >  2:3541852/1‑140 (MQ=255)

                                                                                                                                       act‑ac‑AAAT‑TTGCAAAATACGGG‑AAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCGGGCAGGCCTGAATATTTTTATAACttttt  <  1:1889678/140‑1 (MQ=255)

TTACTATGATTGGCTGAAACGGCTTGCTGAATCGGTGCAGAGCGGGGAAATTTTTAAATTTATACCGATTGAAACAAAGTAAAACAAAA‑ACGCCTGGGAAACCGGGCGTTTTTTTGTT‑CTTTTTTATGTAATTACT‑AC‑AAAT‑TTGAAAAATACGGG‑AAAAACATCCCATTTACTTCCAAAATATTTGATGGTAATTTTATGTAGGTAAAATTTGTATTTGGCCGTTCAGAGGAGGTGAAAAGCGGGCAGGCCTGAATATTTTTATAACTTTTT  >  NZ_CP034943/2991118‑2991390

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`