BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP034943	44,870	2 bp→TC	intergenic (‑51/‑449)	abrB ← / → metG	transition state genes transcriptional regulator AbrB/methionine‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	44,870	0	C	T	99.6%	880.1 / ‑4.5	228	intergenic (‑51/‑450)	abrB/metG	transition state genes transcriptional regulator AbrB/methionine‑‑tRNA ligase
Reads supporting (aligned to +/- strand): ref base C (1/0); new base T (113/114); total (114/114)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.85e-01
*	NZ_CP034943	44,871	0	G	C	99.1%	789.6 / ‑4.8	228	intergenic (‑52/‑449)	abrB/metG	transition state genes transcriptional regulator AbrB/methionine‑‑tRNA ligase
Reads supporting (aligned to +/- strand): ref base G (1/0); major base C (112/113); minor base A (1/1); total (114/114)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 228 total aligned reads displayed.

ATTCCAAGAGTACGACGCAATTCGATAGGAATAACTACACGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCGAC‑AAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAATTAGTTCTTATAGTCAGTAATGTATCAGAAACTACTGAAATAA  >  NZ_CP034943/44737‑45000

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   ccAAGAGTACGACGCAATTCGATAGGAATAACTACACGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCattatt                                                                                                                            >  2:2818338/1‑140 (MQ=255)

              acgCAATTCGATAGGAATAACTACACGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGAC‑AAAATcc                                                                                                                >  1:1819898/1‑140 (MQ=255)

               cgCAATTCGATAGGAATAACTACACGTCCTAGCTCATCAACTTTACGTACAATCACAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGAC‑AAAATCCt                                                                                                               <  2:3939779/140‑1 (MQ=255)

               cgCAATTCGATAGGAATAACTACACGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGTC‑AAAATCCt                                                                                                               <  2:1916268/140‑1 (MQ=255)

               cgCAATTCGATAGGAATAACTACACGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGAC‑AAAATCCt                                                                                                               >  2:3602064/1‑140 (MQ=255)

               cgCAATTCGATAGGAATAACTACACGTCCTAGCTCATCAACTAAACGTACAATACCAGTAGATTTCATAACATTTCTCCTCCCAATAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGAC‑AAAATCCt                                                                                                               >  1:3864152/1‑140 (MQ=255)

               cgCAATTCGATAGGAATAACTACACGTCCTAGCTCATCAAATTTACTTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGAC‑AAAATCCt                                                                                                               <  2:105178/140‑1 (MQ=255)

                gCAATTCGATAGGAATAACTACACGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGAC‑AAAATCCtt                                                                                                              <  1:4191504/140‑1 (MQ=255)

                  aaTTCGATAGGAATAACTACACGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGAC‑AAAATCCTTAc                                                                                                            >  1:3825148/1‑140 (MQ=255)

                  aaTTCGATAGGAATAACTACACGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGAC‑AAAATCCTTAc                                                                                                            >  1:3611078/1‑140 (MQ=255)

                  aaTTCGATAGGAATAACTACACGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGAC‑AAAATCCTTAc                                                                                                            >  1:1476100/1‑140 (MQ=255)

                  aaTTCGATAGGAATAACTACACGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGAC‑AAAATCCTTAc                                                                                                            >  1:1650150/1‑140 (MQ=255)

                     tCGATAGGAATAACTACACGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGAC‑AAAATCCTTACCtt                                                                                                         <  1:1019555/140‑1 (MQ=255)

                     tCGATAGGAATAACTACACGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGAC‑AAAATCCTTACCtt                                                                                                         <  2:2280878/140‑1 (MQ=255)

                       gATAGGAATAACTACACGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGAC‑AAAATCCTTACCTTCa                                                                                                       <  2:694162/140‑1 (MQ=255)

                       gATAGGAATAACTACACGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTACCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGAC‑AAAATCCTTACCTTCa                                                                                                       <  1:860831/140‑1 (MQ=255)

                         tAGGAATAACTACACGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGAC‑AAAATc                                                                                                                 >  1:3828188/1‑128 (MQ=255)

                         tAGGAATAACTACACGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGAC‑AAAATc                                                                                                                 <  2:3828188/128‑1 (MQ=255)

                         tAGGAATAACTACACGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGAC‑AAAATCCTTACCTTCATa                                                                                                     <  1:1372810/140‑1 (MQ=255)

                              aTAACTACACGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGAC‑AAAATCCTTACCTTCATAGCATa                                                                                                >  2:4686351/1‑140 (MQ=255)

                                 aCTACACGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGAC‑AAAATCCTTACCTTCATAGCATAACa                                                                                             >  1:3369420/1‑140 (MQ=255)

                                 aCTACACGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTATCATTATTCGAC‑AAAATCCTTACCTTCATAGCATAACa                                                                                             <  2:1109825/140‑1 (MQ=255)

                                 aCTACACGTCCTAGCTCATCAACTTTACGTACAACACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGAC‑AAAATCCt                                                                                                               <  1:1217739/122‑1 (MQ=255)

                                 aCTACACGTCCTAGCTCATCAACTTTACGTACAACACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGAC‑AAAATCCt                                                                                                               >  2:1217739/1‑122 (MQ=255)

                                      acGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAATTTATATTTATCTTTCTCTTTATTCGAC‑AAAATCCTTACCTTCATAGCATACCAAGGtt                                                                                        >  1:3636084/1‑140 (MQ=255)

                                      acGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGAC‑AAAATCCTTACCTTCATAGCATAACAAGGtt                                                                                        >  2:1737881/1‑140 (MQ=255)

                                       cGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGAC‑AAAATCCTTACCTTCATAGCATAACAAGGttt                                                                                       >  1:2976786/1‑140 (MQ=255)

                                       cGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGAC‑AAAATCCTTACCTTCATAGCATAACAAGGttt                                                                                       >  1:703577/1‑140 (MQ=255)

                                           cTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGAC‑a                                                                                                                      <  1:2675969/105‑1 (MQ=255)

                                           cTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGAC‑AAAATCCTTACCTTCATAGCATAACAAGGTTTCCaa                                                                                   >  2:1591234/1‑140 (MQ=255)

                                           cTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGAC‑AAAATCCTTACCTTCATAGCATAACAAGGTTTCCaa                                                                                   >  2:2634733/1‑140 (MQ=255)

                                             aGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGAC‑AAAATCCTTACCTTCATAGCATAACAAGGTTTCCaata                                                                                 >  1:212427/1‑140 (MQ=255)

                                             aGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGAC‑AAAATCCTTACCTTCATAGCATAACAAGGTTTCCaata                                                                                 >  2:1421142/1‑140 (MQ=255)

                                             aGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGAC‑AAAATCCTTACCTTCATAGCATAACAAGGTTTCCaata                                                                                 >  1:1954343/1‑140 (MQ=255)

                                                  atcaACTTTACTAACAAAAAAATTAAATATAAAAAACTTTCTCCTCCCAAGATATGATTATTTGTTTAAATTATAAATATATTTATAATTATTAGAA‑AAAATAATTAAATTAATATCATAAAAATGTTTAAAATAATctt                                                                            >  2:3796168/1‑138 (MQ=255)

                                                  atcaACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGAC‑AAAATCCTTACCTTCATAGCATAACATGGTTTCCAATAATCGt                                                                            >  1:2929810/1‑140 (MQ=255)

                                                    caACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATGTTTCTCATTATTCGAC‑AAAATCCTTACCTTCATAGCATACAAAGGTTTCCAATAATCGTcg                                                                          >  2:3539067/1‑139 (MQ=255)

                                                    caACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGAC‑AAAATCCTTACCTTCa                                                                                                       >  2:4026372/1‑111 (MQ=255)

                                                    caACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGAC‑AAAATCCTTACCTTCa                                                                                                       <  1:4026372/111‑1 (MQ=255)

                                                    caACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGAC‑AAAATCCTTACCTTCATAGCATAACAAg                                                                                           >  2:3996317/1‑123 (MQ=255)

                                                    caACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGAC‑AAAATCCTTACCTTCATAGCATAACAAg                                                                                           <  1:3996317/123‑1 (MQ=255)

                                                    caACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGAC‑AAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCa                                                                          >  1:2809000/1‑140 (MQ=255)

                                                    caACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGAC‑AAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCa                                                                          >  2:755843/1‑140 (MQ=255)

                                                          tACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGAC‑AAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCAtt                                                                    >  1:3117171/1‑140 (MQ=255)

                                                               aCAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGAC‑AAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATtttgtt                                                               <  2:377836/140‑1 (MQ=255)

                                                                  aTACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGAC‑AAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGc                                                            >  1:1534499/1‑140 (MQ=255)

                                                                  aTACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGAC‑AAAATCCTTACATTCATAGCATAACAAGGTTTCCAATAATCTTCAATCATtttgtttga                                                            >  1:3925439/1‑139 (MQ=255)

                                                                    aCCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGAC‑AAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTa                                                          <  1:3741965/140‑1 (MQ=255)

                                                                        gTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTCTTTGTTTAAATTATATTTATCTTTCTCATTATTCGAC‑AAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTattga                                                      >  1:2064772/1‑140 (MQ=255)

                                                                        gTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGAC‑AAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTattga                                                      >  2:4226933/1‑140 (MQ=255)

                                                                        gTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGAC‑AAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTattga                                                      >  1:2298095/1‑140 (MQ=255)

                                                                        gTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGAC‑AAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTa                                                          <  2:3612621/136‑1 (MQ=255)

                                                                         tAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGAC‑AAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTattgat                                                     >  1:3281408/1‑140 (MQ=255)

                                                                          aGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGAC‑AAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTattgatt                                                    <  2:3864152/140‑1 (MQ=255)

                                                                           gATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGAC‑AAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTattgattg                                                   >  1:952078/1‑140 (MQ=255)

                                                                           gATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGAC‑AAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTattgattg                                                   >  1:2406670/1‑140 (MQ=255)

                                                                            aTTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGAC‑AAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGt                                                  <  2:4112158/140‑1 (MQ=255)

                                                                             tttCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGAC‑AAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTa                                                 <  1:4226933/140‑1 (MQ=255)

                                                                             tttCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGAC‑AAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTa                                                 <  2:1521599/140‑1 (MQ=255)

                                                                             tttCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGAC‑AAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTa                                                 >  1:4367322/1‑140 (MQ=255)

                                                                              ttCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGACAAAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTa                                                 >  1:356440/1‑140 (MQ=255)

                                                                              ttCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGAC‑AAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTaa                                                >  2:1271974/1‑140 (MQ=255)

                                                                              ttCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGAC‑AAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTaa                                                >  2:4006222/1‑140 (MQ=255)

                                                                              ttCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGAC‑AAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTaa                                                >  1:649264/1‑140 (MQ=255)

                                                                              ttCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGAC‑AAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTaa                                                >  1:4436978/1‑140 (MQ=255)

                                                                                 aTAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGAC‑AAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGaa                                             <  2:1031972/140‑1 (MQ=255)

                                                                                   aaaCTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGAC‑AAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAAtt                                           <  2:3117171/140‑1 (MQ=255)

                                                                                   aaaCTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGAC‑AAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAAtt                                           <  2:3424568/140‑1 (MQ=255)

                                                                                    aaCTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGAC‑AAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAATTa                                          <  1:4013741/140‑1 (MQ=255)

                                                                                     aCTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGAC‑AAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATACTCGTCAATCATTTTGTTTGCTATTGATTGTAAGAATTAg                                         <  1:2634733/140‑1 (MQ=255)

                                                                                     aCTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGAC‑AAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAATTAg                                         <  2:2654947/140‑1 (MQ=255)

                                                                                     aCTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGAC‑AAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAATTAg                                         <  2:3281408/140‑1 (MQ=255)

                                                                                          ctcctcCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGAC‑AAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAATTAGTTCtt                                    <  2:952078/140‑1 (MQ=255)

                                                                                          ctcctcCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGAC‑AAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAATTAGTTCtt                                    <  2:4367322/140‑1 (MQ=255)

                                                                                          ctcctcCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGAC‑AAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAATTAGTTCtt                                    <  2:1650150/140‑1 (MQ=255)

                                                                                          ctcctcCCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGAC‑AAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAATTAGTTCtt                                    <  2:2406670/140‑1 (MQ=255)

                                                                                           tcctccCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGAC‑AAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAATTAGTTCTta                                   <  2:1009638/140‑1 (MQ=255)

                                                                                           tcctccCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGAC‑AAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAATTAGTTCTta                                   <  2:2089716/140‑1 (MQ=255)

                                                                                             ctccCAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGAC‑AAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAATTAGTTCTtata                                 <  2:3290523/140‑1 (MQ=255)

                                                                                               cccAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGAC‑AAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAATTAGTTCTTATAGt                               <  2:2809000/140‑1 (MQ=255)

                                                                                               cccAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGAC‑AAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAATTAGTTCTTATAGt                               <  2:4448676/140‑1 (MQ=255)

                                                                                               cccAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGAC‑AAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAATTAGTTCTTATAGt                               <  2:769480/140‑1 (MQ=255)

                                                                                               cccAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGAC‑AAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAATTAGTTCTTATAGt                               <  2:427500/140‑1 (MQ=255)

                                                                                               cccAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGAC‑AAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAATTAGTTCTTATAGt                               <  1:454370/140‑1 (MQ=255)

                                                                                               cccAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGAC‑AAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAATTAGTTCTTATAGt                               <  1:767001/140‑1 (MQ=255)

                                                                                               cccAAGAGATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGAC‑AAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAATTAGTTCTTATAGt                               <  2:1613301/140‑1 (MQ=255)

                                                                                                   agagATGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGAC‑AAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAATTAGTTCTTATAGTCAGt                           >  1:2255576/1‑140 (MQ=255)

                                                                                                    gagaTGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGAC‑AAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAATTAGTTCTTATAGTCAGTa                          <  2:2976786/140‑1 (MQ=255)

                                                                                                    gagaTGATTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGAC‑AAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAATTAGTTCTTATAGTCAGTa                          <  2:649264/140‑1 (MQ=255)

                                                                                                      gatgatTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGAC‑AAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAATTAGTTCTTATAGTCAGTAAt                        <  2:1819898/140‑1 (MQ=255)

                                                                                                      gatgatTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGAC‑AAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAATTAGTTCTTATAGTCAGTAAt                        <  2:2215389/140‑1 (MQ=255)

                                                                                                       atgatTATTTGTTTAAATTATATTTATCTTTCTCATTATTCGAC‑AAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAATTAGTTCTTATAGTCAGTAATg                       >  2:4403081/1‑140 (MQ=255)

                                                                                                          attattTGTTTAAATTATATTTATCTTTCTCATTATTCGAC‑AAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAATTAGTTCTTATAg                                <  1:1044571/128‑1 (MQ=255)

                                                                                                           ttattTGTTTAAATTATATTTATCTTTCTCATTATTCGAC‑AAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAATTAGTTCTTATAGTCAGTAATGTATc                   <  1:1719674/140‑1 (MQ=255)

                                                                                                            tattTGTTTAAATTATATTTATCTTTCTCATTATTCGAC‑AAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAATTAGTTCTTATAGTCAGTAATGTATCa                  >  1:3105631/1‑140 (MQ=255)

                                                                                                              tttGTTTAAATTATATTTATCTTTCTCATTATTCGAC‑AAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAATTAGTTCTTATAGTCAGTAATGTATCAGa                <  2:2064772/140‑1 (MQ=255)

                                                                                                                    tAAATTATATTTATCTTTCTCATTATTCGAC‑AAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTattga                                                      <  2:3255365/96‑1 (MQ=255)

                                                                                                                    tAAATTATATTTATCTTTCTCATTATTCGAC‑AAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTattga                                                      >  1:3255365/1‑96 (MQ=255)

                                                                                                                     aaaTTATATTTATCTTTCTCATTATTCGAC‑AAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAATTAGTTCTTATAGTCAGTAATGTATCAGAAactact         <  1:4686351/140‑1 (MQ=255)

                                                                                                                            atTTATCTTTCTCATTATTCGAC‑AAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAATTAGTTCTTATAGTCAGTAATGTATCAGAAACTACTGAAATaa  >  2:940755/1‑140 (MQ=255)

||

ATTCCAAGAGTACGACGCAATTCGATAGGAATAACTACACGTCCTAGCTCATCAACTTTACGTACAATACCAGTAGATTTCATAAACTTTCTCCTCCCAAGAGATGATTATTTGTTTAAATTATATTTTTCTTCGTCATTATTCGAC‑AAAATCCTTACCTTCATAGCATAACAAGGTTTCCAATAATCGTCAATCATTTTGTTTGCTATTGATTGTAAGAATTAGTTCTTATAGTCAGTAATGTATCAGAAACTACTGAAATAA  >  NZ_CP034943/44737‑45000

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`