BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	4,000,069	C→A	5.7%	F105L (TTC→TTA)	EO946_RS20555 →	MFS transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	4,000,069	0	C	A	5.7%	1797.4 / 14.5	720	F105L (TTC→TTA)	EO946_RS20555	MFS transporter
Reads supporting (aligned to +/- strand): ref base C (178/440); new base A (57/2); total (278/442)
Fisher's exact test for biased strand distribution p-value = 3.86e-26
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 727 total aligned reads displayed.

TTCGTTTGCAGTTCTTTCTCCGCTTGCGCCTAAGCTCGGACAGCGCCTCGGAAATGAGCGAACCTTATGGCTTGGTCTCCTGATCCTGTTTATCGGCGTTTTGATTCGTTCTGCCGGCTATACTGCCGCTTTATTTTTCGGAACGGCACTCATCGGAGTCGG‑AATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTGAAAAGTCAGGGATCATGATCAGCCTGTATACGACATCAATGAATATATTCGCTGCTTTGGCAT  >  NZ_CP034943/3999931‑4000208

  cGTTTGCAGTTCTTTCTCCGCTTGCGCCTAAGCTCGGACAGCGCCTCGGAAATGAGCGAACCTTATGGCTTGGTCTCCTGATCCTGTTTATCGGCGTTTTGATTCGTTCTGCCGGCTATACTGCCGCTTTATTTTTAGGa                                                                                                                                           >  1:6751705/1‑140 (MQ=255)

  cGTTTGCAGTTCTTTCTCCGCTTGCGCCTAAGCTCGGACAGCGCCTCGGAAATGAGCGAACCTTATGGCGTGGTCGCCTGATCCTGTTTATCGGCGTTTTGTTTCGTTCTGCCGGCTATACTGCCGCTTTATTTTTCGGa                                                                                                                                           >  2:2434497/1‑140 (MQ=255)

   gTTTGCAGTTCTTTCTCCGCTTGCGCCTAAGATCGGACATCGCCTCGGTAATGAGCGAACCTTATGGCTTGTTATCCTGATCCTGTTTATCGGCGTTTTGATTCGTTCTGCCGGCTATACTGCCGCTTTATTTTTCGGaa                                                                                                                                          <  1:5429424/140‑1 (MQ=255)

        cAGTTCTTTCTCCGCTTGCGCCTAAGCTCGGACAGCGCCTCGGAAATGAGCGAACCTTATGGCTTGGTCTCCTGATCCTGTTTATCGGCGTTTTGATTCGTTCTGCCGGCTATACTGCCGCTTTATTTTTAGGAACCGCa                                                                                                                                     >  2:4792676/1‑140 (MQ=255)

                                     ggACAGCGCCTCGGAAATGAGCGAACCTTATGGCTTGGTCTCCTGATCCTGTTTATCGGCGTTTTGATTCGTTCTGCCGGCTATACTGCCGCTTTATTTTTAGGTACGGCACTCATCGGAGTCGG‑AATTGCGATCGGaaa                                                                                                       >  2:7189345/1‑140 (MQ=255)

                                     ggACAGCGCCTCGGAAATGAGCGAACCTTATGGCTTGGTCTCCTGATCCTGTTTATCGGCGTTTTGATTCGTTCTGCCGGCTATACTGCCGCTTTATTTTTAGGAACGGCACTCATCGGAGTCGG‑AATTGCGATCGGaaa                                                                                                       >  2:6690579/1‑140 (MQ=255)

                                           cgcCTCGGAAATGAGCGAACCTTATGGCTTGGTCTCGTGATCCTGTTTATCGGCGTTTTGATTCGTTCTGCCGGCTATACTGCCGCTTTATTTTTAGGACCGGCCCTCAACGGAGTGGG‑GAGTGCGATCGGGAAAGttgt                                                                                                 >  1:942933/1‑138 (MQ=255)

                                           cgcCTCGGAAATGAGCGAACCTTATGGCTTGGTCTCCTGATCCTGTTTATCGGCGTTTTGATTCGTTCTGCCGGCTATACTGCCGCTTTATTTTTCGGTAAGGCACTCATCGGGGTCGG‑AATTGGGGTCGGAAACGGTCt                                                                                                 >  2:6818890/1‑140 (MQ=255)

                                           cgcCTCGGAAATGAGCGAACCTTATGGCTTGGTCTCCTGATCCTGTTTATCGGCGTTTTGATTCGTTCTGCCGGCTATACTGCCGCTTTATTTTTCGGAACGGCa                                                                                                                                     <  1:4137795/105‑1 (MQ=255)

                                           cgcCTCGGAAATGAGCGAACCTTATGGCTTGGTCTCCTGATCCTGTTTATCGGCGTTTTGATTCGTTCTGCCGGCTATACTGCCGCTTTATTTTTCGGAACGGCACTCATCGGAGTCGG‑AATTGCGATCGGAAACGTTCt                                                                                                 >  2:850414/1‑140 (MQ=255)

                                           cgcCTCGGAAATGAGCGAACCTTATGGCTTGGTCTCCTGATCCTGTTTATCGGCGTTTTGATTCGTTCTGCCGGCTATACTGCCGCTTTATTTTTCGGAACGGCACTCATCGGAGTCGG‑AATTGCGATCGGAAACGTTCt                                                                                                 >  1:5474559/1‑140 (MQ=255)

                                           cgcCTCGGAAATGAGCGAACCTTATGGCTTGGTCTCCTGATCCTGTTTATCGGCGTTTTGATTCGTTCTGCCGGCTATACTGCCGCTTTAATTTTAGGAAAGGCACTAATCGGCCGCGG‑AAATGCGATCGGaaaacttgt                                                                                                 >  1:5052021/1‑134 (MQ=255)

                                                 ggAAATGAGCGAACCTTATGGCTTGGTCTCCTGATCCTGTTTATCGGCGTTTTGATTCGTTCTGCCGGCTATACTGCCGCTTTATTTTTCGGAACGGCACTCATCGGAGTCGG‑AATTGCGATCGGAAACGTTCTTTTGcc                                                                                           <  1:2545872/140‑1 (MQ=255)

                                                        aGCGAACCTTATGGCTTGGTCTCCTGATCCTGTTTATCGGCGTTTTTATTCGTTCTGCCGGCTATACTGCCGCTTTATTTTTCGGAACGGCACTCTAACGAGTCCG‑CCTTGGGATCCGGAACGGTCTTTTGGCAAGCGTa                                                                                    >  1:4398249/1‑140 (MQ=255)

                                                        aGCGAACCTTATGGCTTGGTCTCCTGATCCTGTTTATCGGCGTTTTGATTCGTTCTGCTGGCTATACTGCCGCTTTATTTTTCGGAACGGCACTCATCGGAGTCGG‑AATTGCGATCTGAAAAGTTCTTTTGCAAAGCTTa                                                                                    >  2:7092309/1‑140 (MQ=255)

                                                        aGCGAACCTTATGGCTTGGTCTCCTGATCCTGTTTATCGGCGTTTTGATTCGTTCTGCCGGCTATACTGGCGCTTTATTTTTTGGAAAGGCACTCATAGGAGTCGG‑AATTGGGATAGGAAACGGTGTTTTTGCAAGCttt                                                                                    >  1:6665093/1‑139 (MQ=255)

                                                          cGAACCTTATGGCTTGGTCTCCTGATCCTGTTTATCGGCGTTTTGATTCGTTCTGCCGGCTATACTGCCGCTTTATTTTTCGGAACGGCACTCATCGGAGTCGG‑AATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAAt                                                                                  <  1:6902757/140‑1 (MQ=255)

                                                          cGAACCTTATGGCTTGGTCTCCTGATCCTGTTTATCGGCGTTTTGATTCGTTCTGCCGGCTATACTGCCGCTTTATTTTTCGGAACCGCACTCATCGGAGTCGG‑AATTTCGAGCGGAAACGTTCTTTTGCCAAGCTTAAt                                                                                  >  2:210698/1‑140 (MQ=255)

                                                          cGAACCTTATGGCTTGGTCTCCTGATCCTGTTTATCGGCGTTTTGATTCGTTCTGCCGGCTATACTGCCGCTTTATTTTTAGGAACGGCACACAACGGAGTCGG‑AATTGCGATAGGAAACGGTCTTTTGCCAATCTTaaa                                                                                  >  1:3846721/1‑139 (MQ=255)

                                                          cGAACCTTATGGCTTGGTCTCCTGATCCTGTTTATCGGCGTTTTGATTAGTTCTGCCGGCTATACTGCCGCTTTATTTTTCGGAAACGCCCTCATACGAATCCG‑GATTGACCTCTGAAACCTTATTTTTCCCAGCATaag                                                                                  >  1:4796207/1‑139 (MQ=255)

                                                            aaCCTTATGGCTTGGTCTCCTGATCCTGTTTATCGGCGTTTTGATTCGTTCTGCCGGCTATACTGCCGCTTTATTTTTCGGAACGGCACTCATCGGAGTCGG‑AATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCa                                                                                <  2:3615202/140‑1 (MQ=255)

                                                            aaCCTTATGGCTTGGTCTCCTGATCCTGTTTATCGGCGTTTTGATTCGTTCGGCCGGCTATACTGCCGCTTTATTTTTCGGAACGGCACTCATCGGAGTCGG‑AATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCa                                                                                <  2:6065221/140‑1 (MQ=255)

                                                             aCCTTATGGCTTGGTCTCCTGATCCTGTTTATCGGCGTTTTGATTCGTTCTGCCGGCTATACTGCCGCTTTTTTTTTGGGCACCGCACACAACGGAGGGGG‑GAGTGCGATCGGCAACGGTGTTTTTCCAAGCTTAATaaa                                                                               >  1:4677925/1‑140 (MQ=255)

                                                             aCCTTATGGCTTGGTCTCCTGATCCTGTTTATCGGCGTTTTGATTCGTTCTGCCGGCGATACTGCCGCTTTATTTTTAGGAACGGCCCGCATCGGAGTCGG‑GATTGCGATCGGAAACCTTATTTTTCCAAGCGTTATCaa                                                                               >  1:5290000/1‑140 (MQ=255)

                                                              ccTTATGGCTTGGTCTCCTGATCCTGTTTATCGGCGTTTTGATTCGTTCTGCCGGCTATACTGCCGCTTTATTTTTCGGAACGGCACTCATCGGAGTCGG‑AATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCaaa                                                                              <  1:7395610/140‑1 (MQ=255)

                                                                    ggCTTGGTCTCCTGATCATGTTTTTCGGCGTTTTGATTCGTTCTGCCGGATATACTGCCGCTTTATTGTTAGGAACGGGACTCATCGGAGTCGG‑AATTGCGATCGTAAACGTTCTTTTGCCAAGCTTAATCAAACATaaa                                                                        <  2:3753778/140‑1 (MQ=255)

                                                                     gCTTGGTCTCCTGATCCTGTTTATCGGCGTTTTGATTCGTTCTGCCGGCTATACTGCCGCTTTATTTTTCGGAACGGCACTCATCGGAGTCGG‑AATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAAt                                                                       <  2:310229/140‑1 (MQ=255)

                                                                         ggTCTCCTGATCCTGTTTATCGGCGTTTTTATTCGTTCTGCCGGCTATAATGCCGCTTTATTTTTTGGAAACGCACGCATAGGCGTGGG‑CAGTTCGATAGGAAAACTTGTTTTTGCAACAGTAATGAAAAATaaaaaaac                                                                   >  1:2665638/1‑135 (MQ=255)

                                                                         ggTCTCCTGATCCTGTTTATCGGCGTTTTGATTCGTTCTGCCGGCTATACTGCCGCTTTATTTTTCGTAACGGCAATAAACGGATTGGG‑AAATTCGGTCGGAAACGTTATTTTGCCAACATTAATCCATCAAAAATAacc                                                                   >  2:1047061/1‑140 (MQ=255)

                                                                         ggTCTCCTGATCCTGTTTATCGGCGTTTTGATTCGTTCTGCCGGCTATACTGCCGCTTTATTTTTCGGTCCGGCACTCAAAGGAGTGGGCAATTG‑GATGGGAAACGTTCTTTTGCCAAGCGTAATCAAACATAAAAAccc                                                                   >  1:7642915/1‑140 (MQ=255)

                                                                         ggTCTCCTGATCCTGTTTATCGGCGTTTTGATTCGTTCTGCCGGCTATACTGCCGCTTTATTTTTCGGAACGGCACTCATCGGAGTGGG‑AAATGCGATCGGAAACGTTCTTTTTCCAAGCTTAATCAAACATAAATAacc                                                                   >  2:517937/1‑140 (MQ=255)

                                                                         ggTCTCCTGATCCTGTTTATCGGCGTTTTGATTCGTTCTGCCGGCTATACTGCCGCTTTATTTTTCGGAACGGCACTCATCGGAGTCGG‑AATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATAccc                                                                   >  2:5721836/1‑140 (MQ=255)

                                                                         ggTCTCCTGATCCTGTTTATCGGCGTTTTGATTCGTTCTGCCGGCTATACTGCCGCTTTATTTTTCGGAACGGCACTCATCGGAGTCGG‑AATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATAccc                                                                   >  2:7670781/1‑140 (MQ=255)

                                                                         ggTCTCCTGATCCTGTTTATCGGCGTTTTGATTCGTTCTGCCGGCTATACTGCCGCTTTATTTTTCGGAACGGCACTCATCGGAGTCGG‑AATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATAacc                                                                   >  2:3902657/1‑140 (MQ=255)

                                                                         ggTCTCCTGATCCTGTTTATCGGCGTTTTGATTCGTTCTGCCGGCTATACTGCCGCTTTATTTTTCGGAACGGCACAAATAGGCGTCGG‑AAATGCGATCGGAAACGTTCTTTTTGCAAACGTAATCAAACATAAAAAccc                                                                   >  2:5532576/1‑140 (MQ=255)

                                                                         ggTCTCCTGATCCTGTTTATCGGCGTTTTGATTCGTTCTGCCGGCTATACTGCCGCTTTATTTTTAGGAAAGGCACTCATCGGAGTGGG‑CATTGCGATCGGGAACCTTATTTTGCCAAGCTTAATAAAACATAAATAccc                                                                   >  1:1692342/1‑140 (MQ=255)

                                                                         ggTCTCCTGATCCTGTTTATCGGCGTTTTGATTCGGTCTGGCGGGTATACTGCCGCTTTATTTTTAGGGAAGGCACGAAAACGCGGGGG‑GCTTGGGATAGGAAAACTTGTTTTTTCAAGCATAAACAAAAATAAATAcac                                                                   >  1:2820114/1‑138 (MQ=255)

                                                                         ggTCTCCTGATCCTGTTTATCGGCGTTTTGATTCGGTCTGCCGGCTATACTGGCGCTTTTTTTTTTGGGACGGCACTCATGGGAGTCGG‑CATTGGGATAGGGAACGTTCTTTTTGCAAGCTTAATAAAAAAAAAATAacc                                                                   >  1:611670/1‑140 (MQ=255)

                                                                             tcCTGATCCTGTTTATCGGCGTTTTGATTCGTTCTGCCGGCTATACTGCCGCTTTATTTTTCGGAACGGCACTCATCGGAGTCGG‑AATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTGaa                                                               <  2:2954974/140‑1 (MQ=255)

                                                                               cTGATCCTGTTTATCGGCGTTTTGATTCGGTCTGCCGGGTATACTGCCGCGTTATTTTTCGGTAAGGCACTCAAAGGAGTCGG‑AAATTCGAACGGTAACGTTCTTTTTGCCAGCTTAATAAAAAATAAATACCCAGaaaa                                                             >  1:5775607/1‑140 (MQ=255)

                                                                                 gATCCTGTTTATCGGCGTTTTGATTCGTTCTGCCGGCTATACTGCCGCTTTATTTTTCGGAACGGCACTCATCGGAGTCGG‑AATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTGAAAAGt                                                           <  1:406395/140‑1 (MQ=255)

                                                                                   tCCTGTTTATCGGCGTTTTGATTCGTTCTGCCGGCTATACTGCCGCTTTATTTTTCGGAACGGCACTCATCGGAGTCGG‑AATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTGAAAAGTCa                                                         <  2:1991827/140‑1 (MQ=255)

                                                                                    ccTGTTTATCGGCGTTTTGATACGTTCTGCCGGCTATACTGCCGCTTTATTTGTCGGAACGGCACTCATCGGAGTCGG‑AAGTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAAGACCCTGAAAAGTCAg                                                        <  1:3342093/140‑1 (MQ=255)

                                                                                       gTTTATCGGCGTTTTGATTCGTTCTGCCGGCTATACTGCCGCTTTATTTTTCGGAACGGCACTCATCGGAGTCGG‑AATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTGAAAAGTCAGGGa                                                     <  2:4198186/140‑1 (MQ=255)

                                                                                       gTTTATCGGCGTTTTGATTCGTTCTGCCGGCGATACTGCCGCGTTATTTTTAGGGACGGCCCACAACGGGGTGGG‑GATTTCGCTCGGAAACGTTGTTTTTGCAAGCGTAATAAAAAAAAAAAAACCAGAAAAGTCAGGGa                                                     >  1:1315116/1‑140 (MQ=255)

                                                                                        tttATCGGCGTTTTGATTCGTTCTGCCGGCTATACTGCCGCTTTATTTTTCGGAACGGCACTCATCGGAGTCGG‑AATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTGAAAAGTCAGGGAt                                                    <  1:5829167/140‑1 (MQ=255)

                                                                                        tttATCGGCGTTTTGATTCGTTCTGCCGGCTATACTGCCGCTTTATTTTTCGGAACGGCACTCATCGGAGTCGG‑AATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTGAAAAGTCAGGGAt                                                    <  2:2488428/140‑1 (MQ=255)

                                                                                         ttATCGGCGTTTTGATTCGTTCTGCCGGCTATACTGCCGCTTTATTTTTCGGAACGGCACTCATCGGAGTCGG‑AAATGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTGAAAAGTCAGGGATc                                                   >  2:6974778/1‑140 (MQ=255)

                                                                                              ggCGTTTTGATTCGTTCTGCCGGCTATACTGCCGCTTTATTTTTCGGAACGGCACTCATCGGAGTCGG‑AATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTGAAAAGTCAGGGATCATGAt                                              <  2:4944184/140‑1 (MQ=255)

                                                                                              ggCGTTTTGATTCGTTCTGCCGGCTATACTGCCGCTTTAGTTTTCGGAACGGCACTCATCGGAGTCGG‑AATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTGAAAAGTCAGGGATCATGAt                                              <  1:6571570/140‑1 (MQ=255)

                                                                                               gCGTTTTGATTCGTTCTGCCGGCTATACTGCCGCTTTATTTTTCGGAACGGCACTCATCGGAGTCGG‑AATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTGAAAAGTCAGGGATCATGATc                                             <  2:1216577/140‑1 (MQ=255)

                                                                                               gCGTTTTGATTCGTTCTGCCGGCTATACTGCCGCTTTATTTTTCGGAACGGCACTCATCGGAGTCGG‑AATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTGAAAAGTCAGGGATCATGATc                                             <  2:6898320/140‑1 (MQ=255)

                                                                                                cGTTTTGATTCGTTCTGCCGGCTATACTGCCGCTTTATTTTTCGGAACGGCACTCATCGGAGTCGG‑AATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTGAAAAGTCAGGGATCATGATCa                                            <  2:2785979/140‑1 (MQ=255)

                                                                                                  ttttGCTTCGTTCTGCCGGCTATACTGCCGCTTTATTTTTCGGAACGGCACTCATCGGAGTCGG‑AATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTGAAAAGTCAGGGATCATGATCAGc                                          <  2:4866787/140‑1 (MQ=255)

                                                                                                  ttttGATTCGTTCTGCCGGCTATACTGCCGCTTTATTTTTCGGAACGGCACTCATCGGGGTCGG‑AATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTGAAAAGTCAGGGATCATGATCAGc                                          <  1:627930/140‑1 (MQ=255)

                                                                                                  ttttGATTCGTTCTGCCGGCTATACTGCCGCTTTATTTTTCGGAACGGCACTCATCGGAGTCGG‑AATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTGAAAAGTCAGGGATCATGATCAGc                                          <  1:5552144/140‑1 (MQ=255)

                                                                                                  ttttGATTCGTTCTGCCGGCTATACTGCCGCTTTATTTTTCGGAACGGCACTCATCGGAGTCGG‑AATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTGAAAAGTCAGGGATCATGATCAGc                                          <  2:413221/140‑1 (MQ=255)

                                                                                                  ttttGATTCGTTCTGCCGGCTATACTGCCGCTTTATTTTTCGGAACGGCACTCATCGGAGTCGG‑AATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTGAAAAGTCAGGGATCATGATCAGc                                          <  1:4952919/140‑1 (MQ=255)

                                                                                                  ttttGATTCGTTCTGCCGGCTATACTGCCGCTTTATTTTTCGGAACGGCACTCATCGGAGTCGG‑AATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTGAAAAGTCAGGGATCATGATCAGc                                          <  1:7308135/140‑1 (MQ=255)

                                                                                                  ttttGATTCGTTCTGCCGGCTATACTGCCGCTTTATTTTTCGGAACGGCACTCATCGGAGTCGG‑AATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTGAAAAGTCAGGGATCATGATCAGc                                          <  2:3316479/140‑1 (MQ=255)

                                                                                                  ttttGATTCGTTCTGCCGGCTATACTGCCGCTTTATTTTTCGGAACGGCACTCATCGGAGTCGG‑AATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTGAAAAGTCAGGGATCATGATCAGc                                          <  2:2648333/140‑1 (MQ=255)

                                                                                                  ttttGATTCGTTCTGCCGGCTATACTGCCGCTTTATTTTTCGGAACGGCACTCATCGGAGTCGG‑AATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTGAAAAGTCAGGGATCATGATCAGc                                          <  1:233724/140‑1 (MQ=255)

                                                                                                  ttttGATTCGTTCTGCCGGCTATACTGCCGCTTTATTTTTCGGAACGGCACTCATCGGAGTCGG‑AATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTGAAAAGTCAGGGATCATGATCAGc                                          <  1:2129985/140‑1 (MQ=255)

                                                                                                  ttttGATTCGTTCTGCCGGCTATACTGCCGCTTTATTTTTCGGAACGGCACTCATCGGAGTCGG‑AATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTGAAAAGTCAGGGATCATGATCAGc                                          <  2:1891773/140‑1 (MQ=255)

                                                                                                  ttttGATTCGTTCTGCCGGCTATACTGCCGCTTTATTTTTCGGAACGGCACTCATCGGAGTCGG‑AATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTGAAAAGTCAGGGATCATGATCAGc                                          <  1:1792854/140‑1 (MQ=255)

                                                                                                  ttttGATTCGTTCTGCCGGCTATACTGCCGCTTTATTTTTCGGAACGGCACTCATCGGAGTCGG‑AATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTGAAAAGTCAGGGATCATGATCAGc                                          <  1:7127625/140‑1 (MQ=255)

                                                                                                  ttttGATTCGTTCTGCCGGCTATACTGCCGCTTTATTTTTCGGAACGGCACTCATCGGAGTCGG‑AATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAAGACCCTGAAAAGTCAGGGATCATGATCAGc                                          <  2:1410078/140‑1 (MQ=255)

                                                                                                  ttttGATTCGGTCTGCCGGCGATACTGCCGCTTTATGTTTCGGAACGGCACTCATCGGAGTCGG‑AATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTGAAAAGTCAGGGATCATGATCAGc                                          <  2:4444877/140‑1 (MQ=255)

                                                                                                         tCGTTCTGCCGGCTATACTGCCGCTTTATTTTTCGGAACGGCACTCATCTGAGTCGG‑AATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTGAAAAGTCAGGGATCATGATCAGCCTGtata                                   <  1:1042440/140‑1 (MQ=255)

                                                                                                         tCGTTCTGCCGGCTATACTGCCGCTTTATTTTTCGGAACGGCACTCATCGGAGTCGG‑AATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTGAAAAGTCAGGGATCATGATCAGCCTGtata                                   <  1:7020662/140‑1 (MQ=255)

                                                                                                            ttCTGCCGGATATACTGCCGCTTTATTTTTCGGAACGGCACTCATCGGAGTCTG‑AATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTGAAAAGTCAGGGATCATGATCAGCCTGTATAcct                                <  1:2763941/140‑3 (MQ=255)

                                                                                                             tCTGCCGGCTATACTGCCGCTTTATTTTTCGGAACGGCACTCATCGGAGTCGG‑AATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATCCCCTGAAAAGTCAGGGATCATGATCAGCCTGTATACGAc                               <  1:4189730/140‑1 (MQ=255)

                                                                                                              cTGCCGGCTATACTGCCGCTTTATTTTTCGGAACGGCACTCATCGGAGTCGG‑AATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTGAAAAGTCAGGGATCATGATCAGCCTGTATACGACa                              <  2:611670/140‑1 (MQ=255)

                                                                                                                 ccGGCTATACTGCCGCTTTATTTTTCGGAACGGCACTCATCGGAGTCGG‑AATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTGAAAAGTCAGGGATCATGATCAGCCTGTATACGACATCa                           <  1:1549050/140‑1 (MQ=255)

                                                                                                                 ccGGCTATACTGCCGCTTTATTTTTCGGAACGGCACTCATCGGAGTCGG‑AATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTGAAAAGTCAGGGATCATGATCAGCCTGTATACGACATCa                           <  2:1540536/140‑1 (MQ=255)

                                                                                                                 ccGGCTATACTGCCGCTTTATTTTTCGGAACGGCACTCATCGGAGTCGG‑AATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTGAAAAGTCAGGGATCATGATCAGCCTGTATACGACATCa                           <  2:1681915/140‑1 (MQ=255)

                                                                                                                 ccGGCTATACTGCCGCTTTATTTTTCGGAACGGCACTCATCGGAGTCGG‑AATTGCGATCGGAAACGTTCTTTTGCCAAGATTAATCAAACATAAATACCCTGAAAAGTCAGGGATCATGATCAGCCTGTATACGACATCa                           <  2:7383063/140‑1 (MQ=255)

                                                                                                                      tataCTGCCGCTTTATTTTTCGGAACGGCACTCATCGGAGTCGG‑AATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTGAAAAGTCAGGGATCATGATCAGCCTGTATACGACATCAATGaa                      <  2:6439942/140‑1 (MQ=255)

                                                                                                                         aCTGCCGCTTTATTTTTCGGAACGGCACTCATCGGAGTCGG‑AATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTGAAAAGTCAGGGATCATGATCAGCCTGTATACGACATCAATGAatat                   <  1:5660514/140‑1 (MQ=255)

                                                                                                                           tGCCGCTTTATTTTTCGGAACGGCACTCATCGGAGTCGG‑AATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCa                                                                                >  2:5921504/1‑77 (MQ=255)

                                                                                                                           tGCCGCTTTATTTTTCGGAACGGCACTCATCGGAGTCGG‑AATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCa                                                                                <  1:5921504/77‑1 (MQ=255)

                                                                                                                             ccGCTTTATTTTTCGGAACGGCACTCATCGGAGTCGG‑AATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTGAAAAGTCAGGGATCATGATCAGCCTGTAAACGACATCAATTAATATATTc               >  2:5432833/1‑140 (MQ=255)

                                                                                                                             ccGCTTTATTTTTCGGAACGGCACTCATCGGAGTCGG‑AATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTGAAAAGTCACGGATCATGATCAGCCTGTATAAGACATCAATGAATATATTc               >  2:7327258/1‑140 (MQ=255)

                                                                                                                             ccGCTTTATTTTTCGGAACGGCACTCATCGGAGTCGG‑AATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAAAACCCTGAAAAGTCAGGGATCATGATCAGCCTGTATACGACATCAATGAATATATTc               >  2:3018267/1‑140 (MQ=255)

                                                                                                                             ccGCTTTATTTTTCGGAACGGCACTCATCGGAGTCGG‑AATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAAACAAAAATAAATAACCTGAAAAGTAAGGGATAATGATAAGCCTGTAAACGACATAAAAGAATAAATTc               >  2:6295770/1‑140 (MQ=255)

                                                                                                                             ccGCTTTATTTTTAGGAACGGCACTCATAGGAGTCGG‑AATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAAAACCCTGAAAAGTCAGGGATCATGATCAGCCTGTATACGACATCCATGAATATATTc               >  2:2283379/1‑140 (MQ=255)

                                                                                                                              cGCTTTATTTTTCGGAACGGCACTCATCGGAGTCGG‑AATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTGAAAAGTCAGGGATCATGATCAGCCTGTATACGACATCAATGAATATATTCg              <  1:6406235/140‑1 (MQ=255)

                                                                                                                              cGCTTTATTTTTCGGAACGGCACTCATCGGAGTCGG‑AATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTGAAAAGTCAGGGATCATGATCAGCCTGTATACGACATCAATGAATATATTCg              <  2:4698679/140‑1 (MQ=255)

                                                                                                                              cGCTTTATTTTTCGGAACGGCACTCATCGGAGTCGG‑AATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTGAAAAGTCAGGGATCATGATCAGCCTGTATACGACATCAATGAATATATTCg              <  2:3395341/140‑1 (MQ=255)

                                                                                                                              cGCTTTATTCTTCGGACCGGCACGCATCGGCGTCGG‑AATTGCGATCGGAAACGTTCTTGTGCCAAGCTTAATCAAACATAAATACCCTGAAAAGTCAGGGATCATGATCAGCCTGTTTACGACATCAATGAATATATTCg              <  2:6511334/140‑1 (MQ=255)

                                                                                                                               gCTTTATTTTTCGGAACGGCACTCATCGGAGTCGG‑AATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTGAAAAGTCAGGGATCATGATCAGCCTGTATACGACATCAATGAATATATTctc             >  2:4548429/1‑138 (MQ=255)

                                                                                                                                cTTTATTTTTCGGAACGGCACTCATCGGAGTCGG‑AATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTGAAAAGTCAGGGATCATGATc                                             >  2:3481055/1‑107 (MQ=255)

                                                                                                                                  ttATTTTTCGGAACGGCACTCATCGGAGTCGG‑AATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTGAAAAGTCAGGGATCATGATCAGCCTGTATACGACATCAATGAATATATTCgctgc          >  2:753139/1‑140 (MQ=255)

                                                                                                                                   tATTTTTCGGAACGGCACTCATCGGAGTCGG‑AATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTGAAAAGTCAGGGATCATGATCAGCCTGTATACGACATCAATGAATATATTCgctgct         <  1:3568603/140‑1 (MQ=255)

                                                                                                                                    aTTTGTCGGAACGGCACTCATCGGAGTCGG‑AATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTGAAAAGTCAGGGATCATGATCAGCCTGTATACGACATCAATGAATATATTCGCTGCtt        <  1:5173582/140‑1 (MQ=255)

                                                                                                                                       tttCGGAACGGCACTCATCGGAGTCGG‑AATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTGAAAAGTCAGGGATCATGATc                                             <  2:5320855/100‑1 (MQ=255)

                                                                                                                                       tttCGGAACGGCACTCATCGGAGTCGG‑AATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTGAAAAGTCAGGGATCATGATCAGCCTGTATACGACATCAATGAATATATTCGCTGCTTTgg     <  2:4286198/140‑1 (MQ=255)

                                                                                                                                       tgtCGGAACGGCACTCATCGGAGTCGG‑AATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTGAAAAGTCAGGGATCATGATCAGCCTGTATACGACATCAATGAATATATTCGCTGCTTTgg     <  1:1472925/138‑1 (MQ=255)

                                                                                                                                          cGGAACGGCACTCATCGGAGTCGG‑AATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTGAAAAGTCAGGGATCATGATCAGCCTGTATACGACATCAATGAATATATTCGCTGCTTTGGCAt  <  1:3223570/140‑1 (MQ=255)

                                                                                                                                          cGGAACGGCACTCATCGGAGTCGG‑AATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTGAAAAGTCAGGGATCATGATCAGCCTGTATACGACATCAATGAATATAGTCGCTGCTTTGGCAt  >  1:3726364/1‑140 (MQ=255)

TTCGTTTGCAGTTCTTTCTCCGCTTGCGCCTAAGCTCGGACAGCGCCTCGGAAATGAGCGAACCTTATGGCTTGGTCTCCTGATCCTGTTTATCGGCGTTTTGATTCGTTCTGCCGGCTATACTGCCGCTTTATTTTTCGGAACGGCACTCATCGGAGTCGG‑AATTGCGATCGGAAACGTTCTTTTGCCAAGCTTAATCAAACATAAATACCCTGAAAAGTCAGGGATCATGATCAGCCTGTATACGACATCAATGAATATATTCGCTGCTTTGGCAT  >  NZ_CP034943/3999931‑4000208

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`