BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	1,036,330	G→T	6.8%	E59* (GAA→TAA)	EO946_RS05355 →	ABC transporter permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	1,036,330	0	G	T	6.8%	998.0 / 10.1	399	E59* (GAA→TAA)	EO946_RS05355	ABC transporter permease
Reads supporting (aligned to +/- strand): ref base G (204/152); new base T (41/2); total (245/154)
Fisher's exact test for biased strand distribution p-value = 1.72e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 419 total aligned reads displayed.

AAAAATAAGATTATGGCTAAATCTTTTATTATTTCGACTGTCATCACAGTACTGCTTGTTTTAGTTGTAACGAATCTGGAGTCGATCATTTCTTTGTTTCAAGGTGATGATGCGAAAGAAAAAATCGCCGTTGTTGATGAAACA‑AACGAATTATATCCGATTTTCTCGAAGCAGCTGAAAGCGGTGGATACAAACGGTGATCTTGATGTGAAGCAATCAAAGCTATCGGAGGATGAGGCCACAAAGCAAGTCAAAGATGAAACGCTTGATGGCATG  >  NZ_CP034943/1036192‑1036467

aaaaaTAAGATTATGGCTAAATCTTTTATTATTTCGACTGTCATCACAGTACTGCTTGTTTTAGTTGTGACGAATCTGGAGTCGATCATTTCTTTGTTTCAAGGTGATGATGCGAAAGAAAAAATCGCCGTTGTtgatga                                                                                                                                           >  2:1133505/1‑140 (MQ=255)

aaaaaTAAGATTATGGCTAAATCTTTTATTATTTCGACTGTCATCACAGTACTGCTTGTTTTAGTTGTAACGACTCTGGAGTCGATCATTTCTGTGTTTCAAGGTGATGATGCGAAAGAAAAAAACCCCGTTGTtgatga                                                                                                                                           >  2:6655529/1‑140 (MQ=255)

aaaaaTAAGATTATGGCTAAATCTTTTATTATTTCGACTGTCATCACAGTACTGCTTGTTTTAGTTGTAACGAATCTGGAGTCGATCATTTCTTTGTTTCAAGGTGCTGATGCGAAAGAAAAAATCGCCGTTGTtgatgt                                                                                                                                           >  1:6234986/1‑139 (MQ=255)

aaaaaTAAGATTATGGCTAAATCTTTTATTATTTCGACTGTCATCACAGTACTGCGTGTTTTAGTTGTAACGAATCTGGAGTCGATCCTTTCTTTGTTTCAAGGTGATGATGCGCAAGAAAAAATCGCCGGTGTtgatga                                                                                                                                           >  2:7637183/1‑140 (MQ=255)

          ttATGGCTAAATCTTTTATTATTTCGACTGTCATCACAGTACTGCTTGTTTTAGTTGTAACGAATCTGGAGTCGATCATTTCTTTGTTTCAAGGTGATGATGCGAAAGAAAAAATCGCCGTTGTTGATGAAACA‑AACGaa                                                                                                                                <  2:6893327/140‑1 (MQ=255)

            aTGGCTAAATCTTTTATTATTTCGACTGTCATCACAGTACTGCTTGTTTTAGTTGTAACGAATCTGGAGTCGATCATTTCTTTGTTTCAAGGTGATGATGCGAAAGAAAAAATCGCCGTTGTTGATGAAACA‑AACGAAtt                                                                                                                              >  2:1019097/1‑140 (MQ=255)

                  agaTCTTTTATTATTTCGACTGTCATCACAGTACTGCTTGTTTTAGTTGTAACGAATCTGGAGTCGATCATTTGTTTTTTTGAAGGTGATGATGCGAAAGAAAAAATCGCCGTTTTTGATGAAACA‑AACGAATTATTTcc                                                                                                                        >  1:4554459/3‑140 (MQ=255)

                   aaTCTTTTATTATTTCGACTGTCATCACAGTACTGCTTGTTTTAGTTGTAACGAATCTGGAGTCGATGATTTCTTTGTGTCAAGGTGATGATGCGATAGCAAAAATCGCCGTTGTTGA‑GCAACACAACGCATGATATcgg                                                                                                                       >  2:4516169/1‑138 (MQ=255)

                     tCTTTTATTATTTCGACTGTCATCACAGTACTGCTTGTTTTAGTTGTAACGAATCTGGAGTCGATCATTTCTTTGTTTCAAGGTGATGATGCGAAAGAAAAAATCGCCGTTGTTGATGAAACA‑AACGAATTATATCCGAt                                                                                                                     <  2:5127293/140‑1 (MQ=255)

                         ttattaTTTCGACTGTCATCACAGTACTGCTGGTTTTAGTTGTAACGAATCTGGAGTCGATCATTTGTTTGTTTGAAGGTGATGATGCGAAAGAAAAAATCCCCGTTGTTGATGAAAAA‑AACGAATTCTATCCGATTTtc                                                                                                                 >  2:6164137/1‑140 (MQ=255)

                          tattatTTCGACTGTCATCACAGTACTGCTTGTTTTAGTTGTAACGAATCTGGAGTCGATCATTTCTTTGTTTCAAGGTGATGATGCGAAAGAAAAAATCGCCGTTGTTGATGAAACA‑AACGAATTATATCCGATTTtct                                                                                                                <  2:7252817/140‑1 (MQ=255)

                               tttataCTTTCATAACAGTACTGCTTGATTTATTTGTAACGAATCTGTATTCGTTCATTTCTTTGTTTCAAGGTGATGATGCGAAAGAAAAAATCGCCGTTGTTGATGAAACA‑AACGAATTATATCCGATTTTCTCGAag                                                                                                           <  1:4468012/135‑1 (MQ=255)

                                 tCGACTGTCATCCCAGTACTGCGTGTTTTAGTTGTAACGAATCTGGAGTCGATCATTTCTTTGTTTCAATGTGATGATGCGAAAGAAAAAAACGCCGTTGGTGATGAACCA‑AACGAAATAAATCCCAGTTTCAAGAagca                                                                                                         >  2:3067739/1‑140 (MQ=255)

                                 tCGACTGTCATCACAGTACTGCTTGTTTTAGTTGTAACGAATCTGGAGTCGATCATTTCTTTTTTTCAAGGTGATGATGCCAAAGAAAAAATAGCCGTTGTTGATTAAACA‑AACGAATTATATCCGATTTTCTCGAagca                                                                                                         >  2:2764831/1‑140 (MQ=255)

                                    aCTGTCATCACAGTACTGCTTGTTTTAGTTGTAACGAATCTGGAGTCGATCATTTCTTTGTTTCAAGGTGATGATGCGAAAGAAAAAATCGCCGTTGTTGTTGAAACA‑AACGAATTATATCCGATTTTCTCGAAGCAGCt                                                                                                      >  2:6509959/1‑140 (MQ=255)

                                    aCTGTCATCACAGTACTGCTTGTTTTAGTTGTAACGAATCTGGAGTCGATCATTTCTTTGTTTCAAGGTGATGATGCGAAAGAAAAAATAGCCGTTGTTGATGAAACA‑AACGCATTATATCCGATTTTCTCGAAGCAGCt                                                                                                      >  2:2072489/1‑140 (MQ=255)

                                      tGTCATCACAGTACTGCTTGTTTTAGTTGTAACGAATCTGGAGTCGATCATTTCTTTGTTTGAAGGTGATGATGCGAAAGAAAAAATCGCCGTTGTTGATGAAACA‑AACGAATTATATCCGATTTTCTCGAAGCAGCTGa                                                                                                    >  2:2209257/1‑140 (MQ=255)

                                       gTCCTCACAGTACTGCTTGTTTTAGTTGTAACGACTCTGGAGTCGATCATTTCTTTGTTTCATGGTGTTGATGCGAAAGAAAAAATCGGCGTTGTTGATGTAACA‑AACGAATTCTATCCGATGTTGTCGAAGCAGCTGag                                                                                                   >  1:7572805/1‑139 (MQ=255)

                                       gTCATCACAGTACTGCTTGTTTTAGTTGTAACGAATCTGGAGTCGATCATTTCTTTGTTTCAAGGTGATGATGCGAAAGAAAAAATCGCCGTTGTTGATGAAACA‑AACGAATTATATCCGATTTTCTCGAAGCAGCTGaa                                                                                                   <  1:4677362/140‑1 (MQ=255)

                                        tcatcaCAGTACTGCTTGTTTTAGTTGTAACGAATCTGGAGTCGATCATTTCTTTGTTTCAAGGTGATGATGCGAAAGAAAAAATCGCCGTTGTTGATGAAACA‑AACGAATTATATCCGATTTTCTCGAAGCAGCTGaaa                                                                                                  <  1:6893385/140‑1 (MQ=255)

                                             acaGTACTGCTTGTTTTAGTTGTAACGAATCTGGAGTCGATCATTTCTTTGTTTCAAGGTGATGATGCGAAAGAAAAAATCGCCGTTGTTGATGAAACA‑AACGAATTATATCCGATTTTCTCGAAGCAGCTGAAAGCGGt                                                                                             <  2:1967219/140‑1 (MQ=255)

                                               aGTACTGCTTGTTTTAGTTGTAACGAATCTGGAGTCGATCATTTGTTTGTTTCAAGGTGATGATGCGAAAGAAAAAATCGCCGTTGTTGATGAAACA‑AACGAATTATATCCGATTTTATCGAAGCAGCTGAAAGCGGTgg                                                                                           >  1:7251017/1‑140 (MQ=255)

                                                  aCTGCTTGTTTTAGTTGTAACGAATCTGGAGTCGATCATTTCTTTGTTTCAAGGTGATGATGCGAAAGAAAAAATCGCCGTTGTTGATTAAACA‑AACGAATTATATCCGATTTTATCGAAGCAGCTGAAAGAGGTGGATa                                                                                        >  2:64780/1‑140 (MQ=255)

                                                  aCTGCTTGTTTTAGTTGTAACGAATCTGGAGTCGATCATTTCTTTGTTTCAAGGTGATGATGCGAAAGAAAAAATCGCCGTTGTTGATGAAAAA‑AACGAATTATATACGATTTTCTCGAAGCAGCTGAAAGCGGTGGATa                                                                                        >  2:6100794/1‑140 (MQ=255)

                                                   cTGCTTGTTTTAGTTGTAACGAATCTGGAGTCGATCATTTCTTTGTTTCAAGGTGATGATGCGAAAGAAAAAATCGCCGTTGTTGATTAAACA‑AACGAATTATATACGATTTTCTCGAAGCAGCTGAAAGCGGTGGATAc                                                                                       >  1:7559231/1‑140 (MQ=255)

                                                    tGCTTGTTTTAGTTGTAACGAATCTGGAGTCGATCCTTTATTTTTTTCAAGGTTTAGAAGCGAAAGAAAAAATACCCGTTTTTTATTAAACA‑AACGCATTTTATACAAATTAGTCGAAGCAGCCTAAAACGGAGGGTACa                                                                                      >  1:2601599/1‑140 (MQ=255)

                                                    tGCTTGTTTTAGTTGTAACGAATCTGGAGTCGATCATTTCTTTGTTTCAAGGTGATGATGCGAAAGAAAAAATCGCCGTTGTTGATGAAACA‑AACGAATTATATCCGATTTTCTCGAAGCAGCTGAAAGCGGTGGATACa                                                                                      <  1:482773/140‑1 (MQ=255)

                                                    tGCTTGTTTTAGTTGTAACGAATCTGGAGTCGATCATTTCTTTGTTTCAAGGTGATGATGCGAAAGAAAAAATCGCCGTTGTTGATGAAACA‑AACGAATTATATCCGATTTTCTCGAAGCAGCTGAAAGCGGTGGATACa                                                                                      <  1:680680/140‑1 (MQ=255)

                                                    tGCTTGTTTTAGTTGTAACGAATCTGGAGTCGATCATTTCTTTGTTTCAAGGTGATGATGCGAAACAAAAAATAGCCCTTGTTGATGAAACA‑AACGAATTATATCCGATTTTCTCGAAGCAGCTGAAAACGGTGGATACa                                                                                      >  2:4390130/1‑140 (MQ=255)

                                                      cTTGTTTTAGTTGTAACGAATCTGGAGTCGATCATTTCTTTTTTTCAAGGTGATGATGCGAAAGAAAAAATCGCCGTTGTTGTTTAAACA‑AACGAATTATATCCGATTTTCTCGAAGCAGCTGAAAGCGGTGGATACaaa                                                                                    >  1:5671012/1‑140 (MQ=255)

                                                      cTTGTTTTAGTTGTAACGAATCTGGAGTCGATCATTTCTTTGTTTGAAGGTGATGATGCGAAAGAAAAAAACGCCGTTGTTGATGAAACA‑AACGTATTATATCCGATTTTATAGAAGCAGCTGAAAACGGTGGATaacaa                                                                                    >  1:1276825/1‑136 (MQ=255)

                                                      cTTGTTTTAGTTGTAACGAATCTGGAGTCGATCATTTCTTTGTTTCAATGTGATGATGCGAAAGAAAAAATCGCCGTTTTTTATGAAAAA‑AACGAATTATATCCGATTTTCTCGAATCAGCTGAAAGCCGTGGATACaaa                                                                                    >  1:3558219/1‑140 (MQ=255)

                                                      cTTGTTTTAGTTGTAACGAATCTGGAGTCGATCATTTCTTTGTTTCAAGGTGGTTATGCGAAAGAAAAAATCGCCGTTGTTGATTAAACA‑AACGAATTATATACGATTTTATAGAAGCAGCTGAAAGCGGTGGGTACcaa                                                                                    >  1:3428719/1‑140 (MQ=255)

                                                      cTTGTTTTAGTTGTAACGAATCTGGAGTCGATCATTTCTTTGTTTCAAGGTGATTATGCGAAAGAAAAAATCGCCGTTGTTGATTAAACA‑AAAGACTTATATCCGATTTTAAAGAAGCAGCTGAAAGCGGTGGATACaaa                                                                                    >  2:3903061/1‑140 (MQ=255)

                                                      cTTGTTTTAGTTGTAACGAATCTGGAGTCGATCATTTCTTTGTTTCAAGGTGATGATGCGAAAGAAAAAATCGCCGTTGTTGATGAAACA‑AACGAATTATATCCGATTTTCTCGAAGCAGCTGAAAGCGGTGGATACaaa                                                                                    >  2:1664063/1‑140 (MQ=255)

                                                      cTTGTTTTAGTTGTAACGAATCTGGAGTCGATCATTTCTTTGTTTCAAGGTGATGATGCGAAAGAAAAAATCGCCGTTGTTGATGAAACA‑AACGAATTATATCCGATTTTCTCGAAGCAGCTGAAAGCGGTGGATACaaa                                                                                    >  2:5043577/1‑140 (MQ=255)

                                                      cTTGTTTTAGTTGTAACGAATCTGGAGTCGATCATTTCTTTGTTTCAAGGTGATGATGCGAAAGAAAAAATCGCCGTTGTTGATGAAACA‑AACGAATTATATACGATTTTCTCGAAGCAGCTGAAAGCGGTGGATACaaa                                                                                    >  2:3343453/1‑140 (MQ=255)

                                                      cTTGTTTTAGTTGTAACGAATCTGGAGTCGATCATTTCTTTGTTTCAAGGTGATGATGCGAAAGAAAAAATCGCCGTTGTTGATGAAACA‑AACGAAGTATATCCGATTTTCTCGACGCAGCTGAAAGCGGTGGATACaaa                                                                                    >  2:890712/1‑140 (MQ=255)

                                                      cTTGTTTTAGTTGTAACGAATCTGGAGTCGATCATTTCTTTGTTTCAAGGTGATGATGCGAAAGAAAAAATCGCCGTTGTTGATGAAACA‑AACCAATTATATCCGATTTTCTCGAAGCAGCTGAAAGCGGTGGATACaaa                                                                                    >  2:5874469/1‑140 (MQ=255)

                                                      cTTGTTTTAGTTGTAACGAATCTGGAGTCGATCATTTCTTTGTTTAAAGGTGATGATGCGAAAGTAAAAATCGCCGTTGTTGATTAAACA‑AACGACTTATATCCGTTTTTCTCGAAGCAGCTGAAAGCGGTgggtgccca                                                                                    >  1:4382139/1‑133 (MQ=255)

                                                      cTTGTTTTAGTTGTAACGAATCTGGAGTCGATCATTTATTTGTTTGAAGGTGATGATGCGAAAGAAAAAATAGCCGTTGTTGATGAAAAA‑AAAGAATTATATGCGATTTTCTCGAAGCAGCTGAAAGCGGTGGATACaaa                                                                                    >  1:2282998/1‑140 (MQ=255)

                                                      cTTGTTTTAGTTGTAACGAATCTGGAGTCGATCATTTATTTGTTTCAATGTGATGATGCGAAAGAAAAAATCGCCGTTGTTGATGAAACA‑AAAGAATTATATCCGATTTTCTAGAAGCAGCTGAAAGCGGTGGATACaaa                                                                                    >  1:296614/1‑140 (MQ=255)

                                                      cTTGTTTTAGTTGTAACGAATCTGGAGTCGATCATTTATTTGTTTCAAGGTGATGATGCGAAAGAAAAAATCGCCGTTTTTGATGAAACA‑AAAGAAATATATACGATTTTATAGAAACACCAGAAACCGGTGGATAaaaa                                                                                    >  1:4642899/1‑140 (MQ=255)

                                                      cTTGTTTTAGTTGTAACGAATCTGGAGTCGATAATTTATTTGTTTGAAGGTGATGATTCGAAAGAAAAAATCGCCGGTGTTGATGAAACA‑AACGAATTATATCCCATTTTCTCGAAGCAGCTGAAAGCGGTGGATACaaa                                                                                    >  2:3614924/1‑140 (MQ=255)

                                                      cTTGTTGTAGTTGTAACGAATCTGGAGTCGATCATTTCTTTATTTCAAGGTGATGATGCGAAAGAAAAAATAGCCGTTGTTGATGAAACA‑AAAGAATTATATCCGATATTCTCGAAGAAGCTGAAAGCTGTTGATACaaa                                                                                    >  2:5978912/1‑140 (MQ=255)

                                                      attGTTTTAGTTGTAACGAATCTGGAGTCGATCATTTATTTGTTTCAAGGTGATGATGCGAAAGAAAAAATCGCCGTTGTTGATTAAACA‑AACCAATTATATACGATTTTCTCGAAGCAGCTGAAAGCGGTGGATACaaa                                                                                    >  1:7085901/2‑140 (MQ=255)

                                                         gTTTTAGTTGTAACGAATCTGGAGTCGATCATTTCTTTGTTTCAAGGTGATGATGCGAAAGAAAAAATCGCCGTTGTTGATGAAACA‑AACGAATTATATCCGATTTTCTAGTATCAGCGGAAAGCGGTGGATACAAACgg                                                                                 >  1:5294089/1‑140 (MQ=255)

                                                         gTTTTAGTTGTAACGAATCTGGAGTCGATCATATCATTTTTTAAAATTTATGATGCGAAAAAAAAAATATCCTTTGTTTATGTAACA‑AACCAAATATATACCAATTTATCCAAGCAGCTGAAAGCTGTGGATACAAACgg                                                                                 >  1:4090606/1‑140 (MQ=255)

                                                           tttAGTTGTAACGAATCTGGAGTCGATCATTTGTTTGTTTACAGGTGATTATGCGAAATAAAAAATCGGCGTTGTTGATGAAACA‑AAAGAATTATATCCGATTTTCTAGAATCAGCTGAAAGCGGTGGATGCAAACGGTg                                                                               >  1:2065154/1‑140 (MQ=255)

                                                           tttAGTTGTAACGAATCTGGAGTCGATCATTTCTTTGTTTCAATGTGATGATGCGAAAGAAAAAATAGCCGTTGTTGATTAAACA‑AACGAATTATATCCGATTTTCTCGCAGCATCTGAAAGCGGTGGATAAAAACCGtt                                                                               >  1:976123/1‑139 (MQ=255)

                                                           tttAGTTGTAACGAATCTGGAGTCGATCATTTCTTTGTTTCAAGGTGTTGATGCGAAAGAAAAAATAGCCGTTGTTGATGAAACA‑AACGAATTATATCCGATTTTCTCGAAGCAGCTGAAAGCAGTGGATACAAACGGTg                                                                               >  2:3749928/1‑140 (MQ=255)

                                                           tttAGTTGTAACGAATCTGGAGTCGATCATTTCTTTGTTTCAAGGTGATTATGCGAAAGAAAAAATCGCCGTTGTTGATGTAACA‑AACGAATTATATACGATTTTCTCGAAGCAGCTGAAAGCGGTGGATACAAACGGTg                                                                               >  1:5977290/1‑140 (MQ=255)

                                                           tttAGTTGTAACGAATCTGGAGTCGATCATTTCTTTGTTTCAAGGTGATGATTCGAAAGAAAAAATAGCCGTTTTTGATTAAACA‑AACGAATTATATACCATTTTATCGAAGCAGCTGAAAGCGGTGGATACAAAAGGTg                                                                               >  1:6354328/1‑140 (MQ=255)

                                                           tttAGTTGTAACGAATCTGGAGTCGATCATTTCTTTGTTTCAAGGTGATGATTCGAAAGAAAAAATAGCCGTTGTTGATGAAACA‑AACGAATTATATCCGATTTTCTAGAAGCAGCTTAAAAAGGTGGATAAAAACAGTg                                                                               >  2:13131/1‑140 (MQ=255)

                                                           tttAGTTGTAACGAATCTGGAGTCGATCATTTCTTTGTTTCAAGGTGATGATGCGAAAGAAAAAATCGCCGTTGTTGATTAAACA‑AACGAATTATATCCGATTTTCTCGAAGCAGCTGAAAGCGGTGGATACAAACGGTg                                                                               >  1:6065689/1‑140 (MQ=255)

                                                           tttAGTTGTAACGAATCTGGAGTCGATCATTTCTTTGTTTCAAGGTGATGATGCGAAAGAAAAAATCGCCGTTGTTGATGAAACA‑AACGAATTATATCCGATTTTCTCGAAGCAGCTGAAAGCGGTGGATACAAACGGTg                                                                               >  2:6790886/1‑140 (MQ=255)

                                                           tttAGTTGTAACGAATCTGGAGTCGATCATTTCTTTGTTTCAAGGTGATGATGCGAAAGAAAAAATCGCCGTTGTTGATGAAACA‑AACGAATTATATCCGATTTTCTCGAAGCAGCTGAAAGCGGTGGATACAAACGGTg                                                                               >  2:2546764/1‑140 (MQ=255)

                                                           tttAGTTGTAACGAATCTGGAGTCGATCATTTCTTTGTTTCAAGGTGATGATGCGAAAGAAAAAATCGCCGTTGTTGATGAAACA‑AACGAATTATATCCGATTTTCTCGAAGCAGCGGTAAGCGGGGGAGACAAACGGTg                                                                               >  2:7689925/1‑140 (MQ=255)

                                                           tttAGTTGTAACGAATCTGGAGTCGATCATTTCTTTGTTTCAAGGTGATGATGCGAAAGAAAAAATCGCCGTTGTTGATGAAACA‑AACGAATTATATACGATTTTCTAGAATCAGCTGAAAGCGGTGGATACAAACGGTg                                                                               >  2:7338420/1‑140 (MQ=255)

                                                                   tAACGAATCTGGAGTCGATCATTTGTTTGTTTGCATGTGATGCTGCGAAAGAAAAAATCGCCGGTGTTGTTGAAACA‑AACGAGTTATATGCGATTTTCTGGAAGCAGCTGCAAGCGGGGGGGACCAACGGGGGTCTTggt                                                                       >  1:6094370/1‑138 (MQ=255)

                                                                   tAACGAATCTGGAGTCGATCATTTCTTTGTTTCAAGGTGATGATGCGAAAGAAAAAATCGCCGTTGTTGATGAAACA‑AACGAATTATATCCGATTTTCTCGAAGCAGCTGAAAGCGGTGGATACAAACGGTTATCTTGAt                                                                       >  2:5332677/1‑140 (MQ=255)

                                                                    aaCGAATCTGGAGTCGATCATTTGTTTTTTTAAAGGTGATTAAGCGAAAGAAAAAAAAGCCGTTGTTGATGAAACA‑AACGAATTATATCCGATTTTCGCGAAGCAGCTGAAACCCGTGGATAAAACAGGTGATGTTGAtt                                                                      >  1:6994231/1‑139 (MQ=255)

                                                                     aCGAATCTGGAGTCGATCATTTCTTTGTTTCAAGGTGATGATGCGAAAGAAAAAATCGCCGTTGTTGATGAAACA‑AACGAATTATATCCGATTTTCTCGAAGCAGCTGAAAGCGGTGGATAAAAACGGTGATCTTGAtgt                                                                     >  2:1418623/1‑140 (MQ=255)

                                                                     aCGAATCTGGAGTCGATCATTTCTTTGTTTCAAGGTGATGATGCGAAAGAAAAAATCGCCCTTTTTGATTAAACA‑AACGAATTATATCCGATTTTATCGAAGCAGCTGAAAGCGGTGGATACAAACGGTGATCTTGAtgt                                                                     >  2:3452799/1‑140 (MQ=255)

                                                                        attCTTGAGTCGATCATTTCTTTGTTTCAAGGTTATGATGCGAAAGAAAAAATCGCCGTTGTTGATGAAACA‑AACGAATTATATCCGATTTTCTCGAAGCAGCTGAAAGCGGTGGATACAAACGGTGATCTTGATGTGaa                                                                  <  2:5630272/138‑1 (MQ=255)

                                                                        aaTCTGGAGTCGATCATTTCTTTGTTTCAAGGTGATGATGCGAAAGAAAAAATCGCCGTTGTTGATGAAACA‑AACGAATTATATCCGATTTTCTCGAAGCAGCTGAAAGCGGTGGATACAAACGGTGATCTTGATGTGaa                                                                  <  1:1095034/140‑1 (MQ=255)

                                                                        aaTCTGGAGTCGATCATTTCTTTGTTTCAAGGTGATGATGCGAAAGAAAAAATCGCCGTTGTTGATGAAACA‑AACGAATTATATCCGATTTTCTCGAAGCAGCTGAAAGCGGTGGATACAAACGGTGATCTTGATGTGaa                                                                  <  1:1562943/140‑1 (MQ=255)

                                                                        aaTCTGGAGTCGATCATTTCTTTGTTTCAAGGTGATGATGCGAAAGAAAAAATCGCCGTTGTTGATGAAACA‑AACGAATTATATCCGATTTTCTCGAAGCAGCTGAAAGCGGTGGATACAAACGGTGATCTTGATGTGaa                                                                  <  2:4008035/140‑1 (MQ=255)

                                                                         aTCTGGAGTCGATCATTTCCTTGTTTCAAGGTGATGATGCGAAAGAAAAAATCGCCGTTGTTGATGAAACA‑AACGAATTATATCCGATTTTCTCGAAGCAGCTGAAAGCGGTGGATACAAACGGTGATCTTGATGTGAAg                                                                 <  1:358431/140‑1 (MQ=255)

                                                                          tCTGGAGTCGATCATTTCTTTGTTTCAATGTGATGATGCGAAAGAAAAAATCGCCGTTTTTGATTAAACA‑AACCAATTATATACGATTTTATAGAAGAAGCTGAAATCGGTTGATACAAAAGGTTATATTTATGTTaaac                                                                >  2:5826819/1‑138 (MQ=255)

                                                                               aGTCGATCATTTCTTTGTTTCAAGGTGATGATGCGAAAGAAAAAATCGCCGTTGTTGATGAAACA‑AACGAATTATATCCGATTTTCTCGAAGCAGCTGAAAGCGGTGGATACAAACGGTGATCTTGATGTGAAGCAATCa                                                           <  2:4927782/140‑1 (MQ=255)

                                                                                      cATTTCTTTGTTTCAAGGTGATGATGCGAAAGAAAAAATCGCCGTTGTTGATGAAACA‑AACGAATTATATCCGATTTTCTCGAAGCAGCTGAAAGCGGTGGATACAAACGGTGATCTTGATGTGAAGCAATCAAAGCTAt                                                    <  2:4250962/140‑1 (MQ=255)

                                                                                        tttCTTTGTTTCAAGGTGATGATGCGAAAGAAAAAATCGCCGTTGTTGATGAAACA‑AACGAATTATATCCGATTTTCTCGAAGCAGCTGAAAGCGGTGGATACAAACGGTGATCTTGATGTGAAGCAATCAAAGCTATCg                                                  <  1:6600387/140‑1 (MQ=255)

                                                                                        tttCTTTGTTTCAAGGTGATGATGCGAAAGAAAAAATCGCCGTTGTTGATGAAACA‑AACGAATTATATCCGATTTTCTCGAAGCAGCTGAAAGCGGTGGATACAAACGGTGATCTTGATGTGAAGCAATCAAAGCTATCg                                                  <  2:5387005/140‑1 (MQ=255)

                                                                                           cTTTGTTTCAAGGTGATGATGCGAAAGAAAAAATCGCCGTTGTTGATGAAACA‑AACGAATTATATCCGATTTTCTCGAAGCAGCTGAAAGCGGTGGATACAAACGGTGATCTTGATGTGAAGCAATCAAAGCTATCggag                                               >  2:5472497/1‑140 (MQ=255)

                                                                                              tGTTTCAAGGTGATGATGCGAAAGAAAAAATCGCCGTTGTTGATGAAACA‑AACGAATTATATCCGATTTTCTCGAAGCAGCTGAAAGCGGTGGATACAAACGGTGATCTTGATGTGAAGCAATCAAAGCTATCGGAGGAt                                            >  1:5207775/1‑140 (MQ=255)

                                                                                                tttCAAGGTGATTATGCGAAAGAAAAAATCGCCGTTGTTGATGAAACA‑AACGAATTATATCCGATTTTCTCGAAGCAGCTGAAAGCGGTGGATACAAACGGTGATCTTGATGTGAAGCAATCAAAGCTATCGGAGGATGa                                          <  1:142493/140‑1 (MQ=255)

                                                                                                tttCAAGGTGATGATGCGAAAGAAAAAATCGCCGTTGTTGATGAAACA‑AACGAATTATATCCGATTTTCTCGAAGCAGCTGAAAGCGGTGGATACAAACGGTGATCTTGATGTGAAGCAATCAAAGCTATCGGAGGATGa                                          <  1:4201437/140‑1 (MQ=255)

                                                                                                   cAAGGTGATGATGCGAAAGAAAAAATCGCCGTTGTTGATGAAACA‑AACGAATTATATCCGATTTTCTCGAAGCAGCTGAAAGCGGTGGATACAAACGGTGATCTTGATGTGAAGCAATCAAAGCt                                                      >  1:1001948/1‑125 (MQ=255)

                                                                                                   cAAGGTGATGATGCGAAAGAAAAAATCGCCGTTGTTGATGAAACA‑AACGAATTATATCCGATTTTCTCGAAGCAGCTGAAAGCGGTGGATACAAACGGTGATCTTGATGTGAAGCAATCAAAGCt                                                      >  1:5777368/1‑125 (MQ=255)

                                                                                                   cAAGGTGATGATGCGAAAGAAAAAATCGCCGTTGTTGATGAAACA‑AACGAATTATATCCGATTTTCTCGAAGCAGCTGAAAGCGGTGGATACAAACGGTGATCTTGATGTGAAGCAATCAAAGCt                                                      <  2:5777368/125‑1 (MQ=255)

                                                                                                     aGGTGATGATGCGAAAGAAAAAATCGCCGTTGTTGATGAAACA‑AACGAATTATATCCGATTTTCTCGAAGCAGCTGAAAGCGGTGGATACAAACGGTGATCTTGATGTGAAGCAATCAAAGCTATCGGAGGATGAGGCca                                     <  2:2837036/140‑1 (MQ=255)

                                                                                                     aGGTGATGATGCGAAAGAAAAAATCGCCGTTGTTGATGAAACA‑AACGAATTATATCCGATTTTCTCGAAGCAGCTGAAAGCGGTGGATACAAACGGTGATCTTGATGTGAAGCAATCAAAGCTATCGGAGGATGAGGCca                                     <  2:187739/140‑1 (MQ=255)

                                                                                                          atgatgCGAAAGAAAAAATCGCCGTTGTTGATGAAACA‑AACGAATTATATCCGATTTTCTCGAAGCAGCTGAAAGCGGTGGATACAAACGGTGATCTTGATGTGAAGCAATCAAAGCTATCGGAGGATGa                                          <  1:3906295/130‑1 (MQ=255)

                                                                                                          atgatgCGAAAGAAAAAATCGCCGTTGTTGATGAAACA‑AACGAATTATATCCGATTTTCTCGAAGCAGCTGAAAGCGGTGGATACAAACGGTGATCTTGATGTGAAGCAATCAAAGCTATCGGAGGATGAGGCCACAAAg                                >  1:4016991/1‑140 (MQ=255)

                                                                                                          atgatgCGAAAGAAAAAATCGCCGTTGTTGATGAAACA‑AACGAATTATATCCGATTTTCTCGAAGCAGCTGAAAGCGGTGGATACAAACGGTGATCTTGATGTGAAGCAATCAAAGCTATCGGAGGATGAGGCCACAAAg                                >  2:7081444/1‑140 (MQ=255)

                                                                                                                cGAAAGAAAAAATCGCCGTTGTTGATGAAACA‑AACGAATTATATCCGATTTTCTCGAagcagc                                                                                                       <  2:4588682/63‑1 (MQ=255)

                                                                                                                cGAAAGAAAAAATCGCCGTTGTTGATGAAACA‑AACGAATTATATCCGATTTTCTCGAAGCAGCTGAAAGCGGGGGATACAAACGGTGATCTTGATGTGAAGCAATCAAAGCTATCGGAGGATGAGGCCACAAAGCAAGTc                          >  1:498874/1‑140 (MQ=255)

                                                                                                                       aaaaaTCGCCGTTGTTGATGAAACA‑AACGAATTATATCCGATTTTCTCGAAGCAGCTGAAAGCGGTGGATACAAACGGTGATCTTGATGTGAAGCAATCAAAGCTATCGGAGGATGAGGCCACAAAGCAAGTCAAAGATg                   <  1:5448989/140‑1 (MQ=255)

                                                                                                                         aaaTCGCCGTTGTTGATGAAACA‑AACGAATTATATCCGATTTTCTCGAAGCAGCTGAAAGCGGTGGATACAAACGGTGATCTTGATGTGACGCAATCAAAGCTATCGGAGGATGAGGCCACAAAGCAAGTCAAAGATGaa                 <  1:31181/140‑1 (MQ=255)

                                                                                                                         aaaTCGCCGTTGTTGATGAAACA‑AACGAATTATATCCGATTTTCTCGAAGCAGCTGAAAGCGGTGGATACAAACGGTGATCTTGATGTGAAGCAATCAAAGCTATCGGAGGATGAGGCCACAAAGCAAGTCAAAGATGaa                 <  1:2673777/140‑1 (MQ=255)

                                                                                                                         aaaTCGCCGTTGTTGATGAAACA‑AACGAATTATATCCGATTTTCTCGAAGCAGCTGAAAGCGGTGGATACAAACGGTGATCTTGATGTGAAGCAATCAAAGCTATCGGAGGATGAGGCCACAAAGCAAGTCAAAGATGaa                 <  2:7181392/140‑1 (MQ=255)

                                                                                                                         aaaTCGCCGTTGTTGATGAAACA‑AACGAATTATATCCGATTTTCTCGAAGCAGCTGAAAGCGGTGGATACAAACGGTGATCTTGATGTGAAGCAATCAAAGCTATCGGAGGATGAGGCCACAAAGCAAGTCAAAGATGaa                 <  2:755206/140‑1 (MQ=255)

                                                                                                                         aaaTCGCCGTTGTTGATGAAACA‑AACGAATTATATCCGATTTTCTCGAAGCAGCTGAAAGCGGTGGATACAAACGGTGATCTTGATGTGAAGCAATCAAAGCTATCGGAGGATGAGGCCACAAAGCAAGTCAAAGATGaa                 <  1:7172873/140‑1 (MQ=255)

                                                                                                                          aaTCGCCGTTGTTGATGAAACA‑AACGAATTATATCCGATTTTCTCGAAGCAGCTGAAAGCGGTGGATACAAACGGTGATCTTGATGTGAAGCAATCAAAGCTATCGGAGGATGAGGCCACAAAGCAAGTCAAAGATGaaa                <  2:2386136/140‑1 (MQ=255)

                                                                                                                           aTCGCCGTTGTTGATGAAACA‑AACGAATTATATCCGATTTTCTCGAAGCAGCTGAAAGCGGTGGATACAAACGGTGATCTTGATGTGAAGCAATCAAAGCTATCGGAGTATGAGGCCACAAAGCAAGTCAAAGATGAAAc               <  1:1191079/140‑1 (MQ=255)

                                                                                                                             cGCCGTTGTTGATGAAACA‑AACGAATTATATCCGATTTTCTCGAAGCAGCTGAAAGCGGTGGATACAAACGGTGATCTTGATGTGAAGCAATCAAAGCTATCGGAGGATGAGGCCACAAAGCAAGTCAAAGATGAAACGc             <  1:1967097/140‑1 (MQ=255)

                                                                                                                              gCCGTTGTTGATGAAACA‑AACGAATTATATCCGATTTTCTCGAAGCAGCTGAAAGCGGTGGATACAAACGGTGATCTTGATGTGAAGCAATCAAAGCTATCGGAGGATGAGGCCACAAAGCAAGTCAAAGATGAAACGCt            <  2:480675/140‑1 (MQ=255)

                                                                                                                                cGTTGTTGATGAAACA‑AACGAATTATATCCGATTTTCTCGAAGCAGCTGAAAGCGGTGGATACAAACGGTGATCTTGATGTGAAGCAATCAAAGCTATCGGAGGATGAGGCCACAAAGCAAGTCAAAGATGAAACGCTTg          <  1:832614/140‑1 (MQ=255)

                                                                                                                                cGTTGTTGATGAAACA‑AACGAATTATATCCGATTTTCTCGAAGCAGCTGAAAGCGGTGGATACAAACGGTGATCTTGATGTGAAGCAATCAAAGCTATCGGAGGATGAGGCCACAAAGCAAGTCAAAGATGAAACGCTTg          >  1:7430469/1‑140 (MQ=255)

AAAAATAAGATTATGGCTAAATCTTTTATTATTTCGACTGTCATCACAGTACTGCTTGTTTTAGTTGTAACGAATCTGGAGTCGATCATTTCTTTGTTTCAAGGTGATGATGCGAAAGAAAAAATCGCCGTTGTTGATGAAACA‑AACGAATTATATCCGATTTTCTCGAAGCAGCTGAAAGCGGTGGATACAAACGGTGATCTTGATGTGAAGCAATCAAAGCTATCGGAGGATGAGGCCACAAAGCAAGTCAAAGATGAAACGCTTGATGGCATG  >  NZ_CP034943/1036192‑1036467

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`