BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	2,350,843	T→G	6.3%	pseudogene (938/1182 nt)	EO946_RS12020 ←	IS3 family transposase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	2,350,843	0	T	G	6.3%	1291.5 / 11.7	453	pseudogene (938/1182 nt)	EO946_RS12020	IS3 family transposase
Reads supporting (aligned to +/- strand): ref base T (61/344); new base G (46/2); total (107/346)
Fisher's exact test for biased strand distribution p-value = 1.39e-30
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 480 total aligned reads displayed.

GAGCTCTTTTTCGGTTTGACACGTTGAAAAATATGCATTCTCTGTTTTGAGATGCGAGAAAAACCATTCGATACAGGCATTATCTAGGCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGATAT‑GCTTTTGTTGTGTATTGGAAGCCTTGGTCAGAATGGAGGATAGCTCCTTGGACGT‑CCCGTTGGGCTGTCATACTTTCCACTGTCTTCATGACCAGTTCAAGATCATTTCGTTTTGACAGCTTCCATGCGAC  >  NZ_CP034943/2350704‑2350982

gAGCTCTTTTTCGGTTTGACACGTTGAAAAATATGCATTCTCTGTTTTGAGATGCGAGAAAAACCATACGATACAGGCATTATCTAGGCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCt                                                                                                                                               >  2:3304141/1‑140 (MQ=255)

   ctctTTTTCGGTTTGACACGTTGAAAAATATGCATTCTCTGTTTTGAGATGCGAGAAAAACCATTCGATACAGGCATTATCTAGGCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCtttt                                                                                                                                            >  2:5965360/1‑140 (MQ=255)

   ctctTTTTCGGTTTGACACGTTGAAAAATATGCATTCTCTGTTTTGAGATGCGAGAAAAACCATTCGATACAGGCATTATCTAGGCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCtttt                                                                                                                                            >  2:2263428/1‑140 (MQ=255)

   ctctTTTTCGGTTTGACACGTTGAAAAATATGCATTCTCTGTTTTGAGATGCGAGAAAAACCATTCGATACAGGCATTATCTAGGCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCgttt                                                                                                                                            >  1:1082650/1‑140 (MQ=255)

   ctctTTTTCGGTTTGACACGTTGAAAAATATGCATTCTCTGTTTTGAGATGCGAGAAAAACCATTCGATACAGGCATTATCTAGGCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCgttt                                                                                                                                            >  2:3702850/1‑140 (MQ=255)

   ctctTTTTCGGTTTGACACGTTGAAAAATATGCATTCTCTGTTTTGAGATGCGAGAAAAACCATTCGATACAGGCATTATCTAGGCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCgttt                                                                                                                                            >  1:7259741/1‑140 (MQ=255)

     ctTTTTCGGTTTGACACGTTGAAAAATATGCATTCTCTGTTTTGAGATGCGAGAAAAACCATTCGATACAGGCATTATCTAGGCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGa                                                                                                                                          <  2:6040506/140‑1 (MQ=255)

     ctTTTTCGGTTTGACACGTTGAAAAATATGCATTCTCTGTTTTGAGATGCGAGAAAAACCATTCGATACAGGCATTATCTAGGCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGa                                                                                                                                          <  2:4156030/140‑1 (MQ=255)

       ttttCGGTTTGACACGTTGAAAAATATGCATTCTCTGTTTTGAGATGCGAGAAAAACCATTCGATACAGGCATTATCTAGGCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGata                                                                                                                                        <  2:4233666/140‑1 (MQ=255)

       ttttCGGTTTGACACGTTGAAAAATATGCATTCTCTGTTTTGAGATGCGAGAAAAACCATTCGATACAGGCATTATCTAGGCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGata                                                                                                                                        <  2:1153801/140‑1 (MQ=255)

       ttttCGGTTTGACACGTTGAAAAATATGCATTCTCTGTTTTGAGATGCGAGAAAAACCATTCGATACAGGCATTATCTAGGCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGata                                                                                                                                        <  1:2525255/140‑1 (MQ=255)

            ggTTTGACACGTTGAAAAATATGCATTCTCTGTTTTGAGATGCGAGAAAAACCATTCGATACAGGCATTATCTAGGCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGATAT‑GCtt                                                                                                                                  <  1:3755609/140‑1 (MQ=255)

             gTTTGACACGTTGAAAAATATGCATTCTCTGTTTTGAGATGCGAGAAAAACCATTCGATACAGGCATTATCTAGGCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGATAT‑GAttt                                                                                                                                 >  2:174419/1‑140 (MQ=255)

               ttGACACGTTGAAAAAGATGCATTCTCTGTTTTGAGATGCGAGAAAAACCATTCGATACAGGCATTATCTAGGCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGATAT‑GCTTttg                                                                                                                               <  1:4545587/140‑1 (MQ=255)

                tGACACGTTGAAAAATATGCATTCTCTGTTTTGAGATGCGAGAAAAACCATTCGATACAGGCATTATCTAGGCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTTGTAT‑TCTTttgt                                                                                                                              >  1:6004214/1‑140 (MQ=255)

                 gACACGTTGAAAAATATGCATTCTCTGTTTTGAGATGCGAGAAAAACCATTCGATACAGGCATTATCTAGGCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGATAT‑GCTTttgtt                                                                                                                             <  1:3079247/140‑1 (MQ=255)

                 gACACGTTGAAAAATATGCATTCTCTGTTTTGAGATGCGAGAAAAACCATTCGATACAGGCATTATCTAGGCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGATAT‑GCTTttgtt                                                                                                                             <  1:922387/140‑1 (MQ=255)

                 gACACGTTGAAAAATATGCATTCTCTGTTTTGAGATGCGAGAAAAACCATTCGATACAGGCATTATCTAGGCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGATAT‑GCTTttgtt                                                                                                                             <  2:1817094/140‑1 (MQ=255)

                 gACACGTTGAAAAATATGCATTCTCTGTTTTGAGATGCGAGAAAAACCATTCGATACAGGCATTATCTAGGCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGATAT‑GCTTttgtt                                                                                                                             <  1:4794623/140‑1 (MQ=255)

                 gACACGTTGAAAAATATGCATTCTCTGTTTTGAGATGCGAGAAAAACCATTCGATACAGGCATTATCTAGGCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGATAT‑GCTTttgtt                                                                                                                             <  2:4460615/140‑1 (MQ=255)

                 gACACGTTGAAAAATATGCATTCTCTGTTTTGAGATGCGAGAAAAACCATTCGATACAGGCATTATCTAGGCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGATAT‑GCTTttgtt                                                                                                                             <  1:1965763/140‑1 (MQ=255)

                  acacGTTGAAAAATATGCATTCTCTGTTTTGAGATGCGAGAAAAACCATTCGATACAGGCATTATCTAGGCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGATAT‑GCTTttgttg                                                                                                                            <  2:5082870/140‑1 (MQ=255)

                       ttGAAAAATATGCATTCTCTGTTTTGAGATGCGAGAAAAACCATTCGATACAGGCATTATCTAGGCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCGTTTTATAT‑GCTTTTGTTTTGTAt                                                                                                                       >  2:4767690/1‑140 (MQ=255)

                          aaaaaTATGCATTCTCTGTTTTGAGATGCGAGAAAAACCATTCGATACAGGCATTATCTAGGCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGATAT‑GCTTTTGTTGTGTATTgg                                                                                                                    <  1:4170622/140‑1 (MQ=255)

                          aaaaaTATGCATTCTCTGTTTTGAGATGCGAGAAAAACCATTCGATACAGGCATTATCTAGGCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGATAT‑GCTTTTGTTGTGTATTgg                                                                                                                    <  1:2684598/140‑1 (MQ=255)

                          aaaaaTATGCATTCTCTGTTTTGAGATGCGAGAAAAACCATTCGATACAGGCATTATCTAGGCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGATAT‑GCTTTTGTTGTGTATTgg                                                                                                                    <  1:1424279/140‑1 (MQ=255)

                          aaaaaTATGCATTCTCTGTTTTGAGATGCGAGAAAAACCATTCGATACAGGCATTATCTAGGCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGATAT‑GCTTTTGTTGTGTATTgg                                                                                                                    <  1:1002079/140‑1 (MQ=255)

                          aaaaaTATGCATTCTCTGTTTTGAGATGCGAGAAAAACCATTCGATACAGGCATTATCTAGGCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGATAT‑GCTTTTGTTGTGTATTgg                                                                                                                    <  2:159930/140‑1 (MQ=255)

                               tatGCATTCTCTGTTTTGAGATGCGAGAAAAACCATTCGATACAGGCATTATCTAGGCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGATAT‑GCTTTTGTTGTGTATTGGAAGcc                                                                                                               <  1:742650/140‑1 (MQ=255)

                               tatGCATTCTCTGTTTTGAGATGCGAGAAAAACCATTCGATACAGGCATTATCTAGGCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGATAT‑GCTTTTGTTGTGTATTGGAAGcc                                                                                                               <  2:5558779/140‑1 (MQ=255)

                               tatGCATTCTCTGTTTTGAGATGCGAGAAAAACCATTCGATACAGGCATTATCTAGGCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGATAT‑GCTTTTGTTGTGTATTGGAAGcc                                                                                                               <  1:3494153/140‑1 (MQ=255)

                                atGCATTGTCTGTTTTGAGATGCGAGAAAAACCATTCGATACAGGCATTATCTAGGCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTTTTAT‑GGGTTTGTGTGGGATTGGTAGccg                                                                                                              >  2:6457716/1‑139 (MQ=255)

                                 tGCATTCTCTGTTTTGAGATGCGAGAAAAACCATTCGATACAGGCATTATCTAGGCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCGTTTGTTAT‑TGTTTTGTTTTGTTTTTGAAGccgt                                                                                                             >  1:7001159/1‑138 (MQ=255)

                                    gagacaggTTTTGAGATGCGAGAAAAACCATTCGATACAGGCATTATCTAGGCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGATAT‑GCTTTTGTTGTGTATTGGAAGCCTTGGt                                                                                                          <  1:7648279/133‑1 (MQ=255)

                                    gagacaggTTTTGAGATGCGAGAAAAACCATTCGATACAGGCATTATCTAGGCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGATAT‑GCTTTTGTTGTGTATTGGAAGCCTTGGt                                                                                                          <  1:6506894/133‑1 (MQ=255)

                                    aTTCTCTGTTTTGAGATGCGAGAAAAACCATTCGATACAGGCATTATCTAGGCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGATAT‑GCTTTTGTTGTGTATTGGAAGCCTTGGt                                                                                                          <  1:3302177/140‑1 (MQ=255)

                                    aTTCTCTGTTTTGAGATGCGAGAAAAACCATTCGATACAGGCATTATCTAGGCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGATAT‑GCTTTTGTTGTGTATTGGAAGCCTTGGt                                                                                                          <  2:3455058/140‑1 (MQ=255)

                                    aTTCTCTGTTTTGAGATGCGAGAAAAACCATTCGATACAGGCATTATCTAGGCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGATAT‑GCTTTTGTTGTGTATTGGAAGCCTTGGt                                                                                                          <  1:4277221/140‑1 (MQ=255)

                                    aTTCTCTGTTTTGAGATGCGAGAAAAACCATTCGATACAGGCATTATCTAGGCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGATAT‑GCTTTTGTTGTGTATTGGAAGCCTTGGt                                                                                                          <  2:74870/140‑1 (MQ=255)

                                    aTTCTCTGTTTTGAGATGCGAGAAAAACCATTCGATACAGGCATTATCTAGGCAGTTTGCCTTACGGGAATGGCTGCCTGTCAAACCAACGGTTTCGAGTCGCTTTTGATAT‑GCTTTTGTTGTGTATTGGAAGCCTTGGt                                                                                                          <  1:5226918/140‑1 (MQ=255)

                                      tctcTGTTTTGAGATGCGAGAAAAACCATTCGATACAGGCATTATCTAGGCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGATAT‑GCTTTTGTTGTGTATTGGAAGCCTTGGTCa                                                                                                        <  2:1428999/140‑1 (MQ=255)

                                           gTTTTGAGATGCGAGAAAAACCATTCGATACAGGCATTATCTAGGCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGATAT‑GCTTTTGTTGTGTATTGGAAGCCTTGGTCAGAATg                                                                                                   <  1:1340728/140‑1 (MQ=255)

                                           gTTTTGACATGCGAGAAAAACCAGTCGATACCGGCATTATCTAGGCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGATAT‑GCTTTTGTTGTGTATTGGAAGCCTTGGTCAGAATg                                                                                                   <  1:5093635/140‑1 (MQ=255)

                                             tttGAGATGCGAGAAAAACCATTCGATACAGGCATTATCTAGGCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGATATGGATTTTGTT‑TGTATTTTAAGCCTTTGTCAGAATggt                                                                                                 >  2:1029016/1‑139 (MQ=255)

                                              ttGAGATGCGAGAAAAACCATTCGATACAGGCATTATCTAGGCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTTTTAT‑GCTTTTGTTTTGTATTGGAAGGCGTGGTGAGAATtgag                                                                                                >  2:126433/1‑140 (MQ=255)

                                                 agaTGCGAGAACAACCATTCGATACAGGCATTATCTAGGCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGATAT‑GCTTTTGTTGTGTATTGGAAGCCTTGGTCAGAATGGAGGAt                                                                                             <  2:7437692/140‑1 (MQ=255)

                                                 agaTGCGAGAAAAACCATTCGATACAGGCATTATCTAGGCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGTTAT‑TCTTTTGTTGTTGATTTGTAGCCATGGTGAGAATTGAGGAt                                                                                             >  1:5496775/1‑140 (MQ=255)

                                                 agaTGCGAGAAAAACCATTCGATACAGGCATTATCTAGGCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGATAT‑GCTTTTGTTGTGTATTGGAAGCCTTGGTCAGAATGGAGGAt                                                                                             <  2:7193516/140‑1 (MQ=255)

                                                  gaTGCGAGAAAAACCATTCGATACAGGCATTATCTAGGCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGATAT‑GCTTTTGTTGTGTATTGGAAGCCTTGGTCAGAATGGAGGATa                                                                                            <  2:5308759/140‑1 (MQ=255)

                                                   aTGCGAGAAAAACCATTCGATACAGGCATTATCTAGGCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGATAT‑GCTTTTGTTGTGTATTGGAAGCCTTGGTCAGAATGGAGGATAg                                                                                           <  1:1699840/140‑1 (MQ=255)

                                                      cGAGAAAAACCATTCGATACAGGCATTATCTAGGCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTTATAT‑TCGTTTTTTTTGTATTGGAAGCCATGGTCATAATtgaggctgtctc                                                                                        >  2:6506894/1‑133 (MQ=255)

                                                      cGAGAAAAACCATTCGATACAGGCATTATCTAGGCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTTATAT‑GCTTTTGTTTTTTTTTGGTAGCCTTTGTCAGAATGGAGGATAGCta                                                                                        >  2:2768503/1‑139 (MQ=255)

                                                      cGAGAAAAACCATTCGATACAGGCATTATCTAGGCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGTTTT‑GGGTTTGTTGTGTATTTGAAGCCGTGGTCAGAATTTAGGATAGCTc                                                                                        >  2:5672377/1‑140 (MQ=255)

                                                      cGAGAAAAACCATTCGATACAGGCATTATCTAGGCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCGTTTGGTAT‑GCGTGTGTTGTGTATTTGTAGCCTTGGTCCGAATGGAGGATAGcgc                                                                                        >  1:3842769/1‑138 (MQ=255)

                                                              gccATTCGATACAGGCATTATCTAGGCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGATAT‑GCTTTTGTTGTGTATTGGAAGCCTTGGTCAGAATGGAGGATAGCTCCTTGGACg                                                                                <  1:7131573/139‑1 (MQ=255)

                                                                  ttCGATACAGGCATTATCTAGGCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGATAT‑GGTTTTGGTGTGTAGTTGGAGCCGTGGGGCGCAGTGAGGTTAGCTCCTTGGACGT‑ccc                                                                           >  2:4265482/1‑140 (MQ=255)

                                                                      aTACAGGCATGATCTAGGCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGATAT‑GCTTTTGTTGTGTATTGGAAGCCTTGGTCAGAATGGAGGATAGCTCCTTGGACGT‑CCCGTTg                                                                       <  1:5781136/140‑1 (MQ=255)

                                                                        aCAGGCATTATCTAGGCAGTTTGCCTTACGGGACTGGCTGCCTGTCAAACCAACGGTTTCGAGTCGCTTTTGATAT‑GCTTTTTTTTTTTATTGGAAGCCTTGGTCAGAATGGAGGTTAGCTCCTTGGACTT‑CCCGTTggg                                                                     <  1:2428516/140‑1 (MQ=255)

                                                                        aCAGGCATTATCTAGGCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGATAT‑GCTTTTGTTGTGTATTGGAAGCCTTGGTCAGAATGGAGGATAGCTCCTTGGACGT‑CCCGTTggg                                                                     <  2:4103435/140‑1 (MQ=255)

                                                                        aCAGGCATTATCTAGGCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGATAT‑GCTTTTGTTGTGTATTGGAAGCCTTGGTCAGAATGGAGGATAGCTCCTTGGACGT‑CCCGTTggg                                                                     <  1:2834064/140‑1 (MQ=255)

                                                                        aCAGGCATTATCTAGGCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGATAT‑GCTTTTGTTGTGTATTGGAAGCCTTGGTCAGAATGGAGGATAGCTCCTTGGACGT‑CCCGTTggg                                                                     <  2:3574195/140‑1 (MQ=255)

                                                                        aCAGGCATTATCTAGGCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGATAT‑GCTTTTGTTGTGTATTGGAAGCCTTGGTCAGAATGGAGGATAGCTCCTTGGACGT‑CCCGTTggg                                                                     <  1:5960052/140‑1 (MQ=255)

                                                                          aGGCATTATCTAGGCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCGTTTTTTAT‑TGTTTTTTTTTGTATTTGAAGCCGTTGTGAGAATGGAGGATAGCTACGTTGTCGTCCCCGTTgggt                                                                    >  1:2249652/1‑139 (MQ=255)

                                                                            gCATTATCTAGGCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTGTTGTTTT‑TCGTTTGTTGTGTATTTGGAGCCGTTGTGAGGATGGAGGATAGGTCCTTGGACGT‑CCCGGTGGGgtgt                                                                 >  2:2391290/1‑140 (MQ=255)

                                                                                  tCTAGGCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGATAT‑GCTTTTGTTGTGTATTGGAAGCCTTGGTCAGAATGGAGGATAGCTCCTTGGACGT‑CCCGTTGGGCTGTCATACt                                                           <  1:2309239/140‑1 (MQ=255)

                                                                                  tCTAGGCAGTTTGCCGTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGATAT‑GCTTTTGTTGTGTATTGGAAGCCTTGGTCAGAATGGAGGATAGCTCCTTGGACGT‑CCCGTTGGGCTGTCATACt                                                           <  1:6391982/140‑1 (MQ=255)

                                                                                      ggCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTTATAT‑TCTTTTGTTGTGTATTTGAAGCCGTTGTCAGAAAGGAGGTTAGCTCCATGGAAGT‑GCCGGTGGGCGGTCAAAAtttac                                                       >  2:2859491/1‑138 (MQ=255)

                                                                                      ggCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCGTTTTTTAT‑TGTTTTTTTTTGTTTTTGTAGCCTTTGTGAGAATTGAGGATAGCTCCTTTGACCT‑CCCCGTGGGCTGTGATaaattac                                                       >  1:6262695/1‑134 (MQ=255)

                                                                                      ggCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCGTTTTATAT‑TCTTTTTTTTTGTATTTGAAGCCGTTTTGAGAATGGAGGATATCTCCTTGTTCGT‑CCCCTTGGGCTGTCATAAtttag                                                       >  2:2511284/1‑138 (MQ=255)

                                                                                      ggCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCGTTGTTTTT‑GCGTTTTTGGTGTTTTTTTAGCCTTGGTGTGATTGGGGGGTAGCTCCGTGGTCGT‑GCCGTTGGGCGGTGCTTCTTTcc                                                       >  1:6870453/1‑140 (MQ=255)

                                                                                       gcagcgtGCCTTACGGGACTGGCTGCCTTTCCAACCAACGGTTTCGAGTCGCGTTTGAGAT‑GCTGTTGTTGTTTATTGGAAGCCTTGGTCAGAATGGAGGATAGCTCCTTGGACGT‑CCCGTTGGGCTGTCATACTTTCCa                                                      <  2:4910345/134‑1 (MQ=255)

                                                                                       gCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTTATAT‑TGTTTTGTTGTGTTTTTGTAGCCGTGGTCAGAATGGACGATATCTCCTTGGACGT‑CCCGGTGGGGGGGCATAATTTACa                                                      >  2:7528956/1‑140 (MQ=255)

                                                                                       gCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGATAT‑GCTTTTGTTGTGTATTGGAAGCCTTGGTCAGAATGGAGGATAGCTCCTTGGACGT‑CCCGTTGGGCTGTCATACTTTCCa                                                      <  2:2489108/140‑1 (MQ=255)

                                                                                       gCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGATAT‑GCTTTTGTTGTGTATTGGAAGCCTTGGTCAGAATGGAGGATAGCTCCTTGGACGT‑CCCGTTGGGCTGTCATACTTTCCa                                                      <  2:2676503/140‑1 (MQ=255)

                                                                                        cAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGATAT‑GCTTTTGTTGTGTATTGGAAGCCTTGGTCAGAATGGAGGATAGCTCCTTGGACGT‑CCCGTTGGGCTGTCATACTTTCCAc                                                     >  2:1881364/1‑140 (MQ=255)

                                                                                         aGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGATAT‑GCTTTTGTTGTGTATTGGAAGCCTTGGTCAGAATGGAGGATAGCTCCTTGGACGT‑CCCGTTGGGCTGTCATACTTTCCACt                                                    <  2:3819274/140‑1 (MQ=255)

                                                                                         aGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGATAT‑GCTTTTGTTGTGTATTGGAAGCCTTGGTCAGAATGGAGGATAGCTCCTTGGACGT‑CCCGTTGGGCTGTCATACTTTCCACt                                                    <  2:2003984/140‑1 (MQ=255)

                                                                                          gTTTGCCTTACGGGAATGGCTGCCTTTCAAACCCACGGTTTCGAGTCGCTTTTGATAT‑GCTTTTGTTGTGTATTGGAAGCCTTGGTCAGAATGGAGGATAGCTCCTTGGACGT‑CCCGTTGGGCTGTCATACTTTCCACTg                                                   <  1:5597433/140‑1 (MQ=255)

                                                                                          gTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGATAT‑GCTTTTGTTGTGTATTGGAAGCCTTGGTCAGAATGGAGGATAGCTCCTTGGACGT‑CCCGTTGGGCTGTCATACTTTCCACTg                                                   <  1:671575/140‑1 (MQ=255)

                                                                                          gTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGATAT‑GCTTTTGTTGTGTATTGGAAGCCTTGGTCAGAATGGAGGATAGCTCCTTGGACGT‑CCCGTTGGGCTGTCATACTTTCCACTg                                                   <  2:5159405/140‑1 (MQ=255)

                                                                                           tttGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTTGTAT‑GCTTTTTTTGTGTATTGGAAGCCTTGGTCAGAATGGAGGAGAGCGCCTTTGACGT‑CCCCGTGGGGTGTCATAAtttgaacggt                                                  >  2:3994932/1‑133 (MQ=255)

                                                                                           tttGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCGTTTGATTT‑GCTTTTGTTTTGTATTGGAAGCCGTTGTGCGAATGGAGGCTAGCTGCTTTGACGT‑CCCGTTGGGGTGGGCTAGTTTACACTga                                                  >  2:5860357/1‑139 (MQ=255)

                                                                                                  tACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGATAT‑GCTTTTGTTGTGTATTGGAAGCCTTGGTCAGAATGGAGGATAGCTCCTTGGACGT‑CCCGTTGGGCTGTCATACTTTCCACTGTCTTCATg                                           <  2:635892/140‑1 (MQ=255)

                                                                                                    cGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGATAT‑GCTTTTGTTGTGTATTGGAAGCCTTGGTCAGAATGGAGGATAGCTCCTTGGACGT‑CCCGTTGGGCTGTCATACTTTCCACTGTCTTCATGAc                                         <  2:3032867/140‑1 (MQ=255)

                                                                                                    cGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGATAT‑GCTTTTGTTGTGTATTGGAAGCCTTGGTCAGAATGGAGGATAGCTCCTTGGACGT‑CCCGTTGGGCTGTCATACTTTCCACTGTCTTCATGAc                                         <  2:3183786/140‑1 (MQ=255)

                                                                                                    cGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGATAT‑GCTTTTGTTGTGTATTGGAAGCCTTGGTCAGAATGGAGGATAGCTCCTTGGACGT‑CCCGTTGGGCTGTCATACTTTCCACTGTCTTCATGAc                                         <  2:4633526/140‑1 (MQ=255)

                                                                                                    cGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGATAT‑GCTTTTGTTGTGTATTGGAAGCCTTGGTCAGAATGGAGGATAGCTCCTTGGACGT‑CCCGTTGGGCTGTCATACTTTCCACTGTCTTCATGAc                                         <  1:767465/140‑1 (MQ=255)

                                                                                                    cGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGATAT‑GCTTTTGTTGTGTATTGGAAGCCTTGGTCAGAATGGAGGATAGCTCCTTGGACGT‑CCCGTTGGGCTGTCATACTTTCCACTGTCTTCATGAc                                         <  2:6601363/140‑1 (MQ=255)

                                                                                                    cGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGATAT‑GCTTTTGTTGTGTATTGGAAGCCTTGGTCAGAATGGAGGATAGCTCCTTGGACGT‑CCCGTTGGGCTGTCATACTTTCCACTGTCTTCATGAc                                         <  2:7069838/140‑1 (MQ=255)

                                                                                                    cGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGATAT‑GCTTTTGTTGTGTATTGGAAGCCTTGGTCAGAATGGAGGATAGCTCCTTGGACGT‑CCCGTTGGGCTGTCATACTTTCCACTGTCTTCATGAc                                         <  1:1881364/140‑1 (MQ=255)

                                                                                                    cGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGATAT‑GCTTTTGTTGTGTATTGGAAGCCTTGGTCAGAATGGAGGATAGCTCCTTGGACGT‑CCCGTTGGGCTGTCATACTTTCCACTGTCTTCATGAc                                         <  2:7345026/140‑1 (MQ=255)

                                                                                                                  tttCAACCCCCCGGTTTCGACTCGCGTTTGAGAT‑GCGTTTGTTGTGTATTGGAAGCCTTGGTCAGAATGGAGGATAGCTCCTTGGACGT‑CCCGTTGGGCTGTCATACTTTCCACTGTCTTCATGACCAGTTCAAGATCAt                           <  1:4971454/140‑1 (MQ=255)

                                                                                                                  tttCAAACCAACGGTTTCGAGTCGCGTTTTTTAT‑GCGTTTGTTTTGTATTTGAAGCCTTGGTGAGAATGGAGGATATCTCCGTGGACGT‑CCCGGGGGGCGGTCATACTTTGCCCAGTGTTCATTAACACTTAAAGATAAt                           >  2:6759702/1‑140 (MQ=255)

                                                                                                                  tttCAAACCAACGGTTTCGAGTCGCGTTTTATAT‑GGTTTTGTTGTGTATTGGAAGCCGTGGTCAGGATGGAGGATAGCTGCTTGGACGT‑CCCGGTGGGCGGTCATACTTTACACGGTGTTCATGTCCAGTTAAAGATCAt                           >  2:3473698/1‑140 (MQ=255)

                                                                                                                            aCGGTTTCGAGTCGCTTTTGATAT‑GCTTTTGTTGTGTATTGGAAGCCTTGGTCAGAATGGAGGATAGCTCCTTGGACGT‑CCCGTTGGGCTGTCATACTTTCCACTGTCTTCATGACCAGTTCAAGATCATTTCGTTTTGa                 <  1:2094307/140‑1 (MQ=255)

                                                                                                                            aCGGTTTCGAGTCGCTTTTGATAT‑GCTTTTGTTGTGTATTGGAAGCCTTGGTCAGAATGGAGGATAGCTCCTTGGACGT‑CCCGTTGGGCTGTCATACTTTCCACTGTCTTCATGACCAGTTCAAGATCATTTCGTTTTGa                 <  1:5082967/140‑1 (MQ=255)

                                                                                                                                          cTTTTGATAT‑GCTTTTGTTGTGTATTGGAAGCCTTGGTCAGAATGGAGGATAGCTCCTTGGACGT‑CCCGTTGGGCTGTCATACTTTCCACTGTCTTCATGACCAGTTCAAGATCATTTCGTTTTGACAGCTTCCATGCGa   <  1:3141205/140‑1 (MQ=255)

                                                                                                                                          cTTTTGATAT‑GCTTTTGTTGTGTATTGGAAGCCTTGGTCAGAATGGAGGATAGCTCCTTGGACGT‑CCCGTTGGGCTGTCATACTTTCCACTGTCTTCATGACCAGTTCAAGATCATTTCGTTTTGACAGCTTCCATGCGa   <  2:6262695/140‑1 (MQ=255)

                                                                                                                                          cTTTTGATAT‑GCTTTTGTTGTGTATTGGAAGCCTTGGTCAGAATGGAGGATAGCTCCTTGGACGT‑CCCGTTGGGCTGTCATACTTTCCACTGTCTTCATGACCAGTTCAAGATCATTTCGTTTTGACAGCTTCCATGCGa   <  2:2575794/140‑1 (MQ=255)

                                                                                                                                           ttttGATAT‑GCTTTTGTTGTGTATTGGAAGCCTTGGTCAGAATGGAGGATAGCTCCTTGGACGT‑CCCGTTGGGCTGTCATACTTTCCACTGTCTTCATGACCAGTTCAAGATCATTTCGTTTTGACAGCTTCCATGCGAc  <  2:6908879/140‑1 (MQ=255)

GAGCTCTTTTTCGGTTTGACACGTTGAAAAATATGCATTCTCTGTTTTGAGATGCGAGAAAAACCATTCGATACAGGCATTATCTAGGCAGTTTGCCTTACGGGAATGGCTGCCTTTCAAACCAACGGTTTCGAGTCGCTTTTGATAT‑GCTTTTGTTGTGTATTGGAAGCCTTGGTCAGAATGGAGGATAGCTCCTTGGACGT‑CCCGTTGGGCTGTCATACTTTCCACTGTCTTCATGACCAGTTCAAGATCATTTCGTTTTGACAGCTTCCATGCGAC  >  NZ_CP034943/2350704‑2350982

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`