BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NZ_CP034943	3,285,713	G→A	31.1%	L230L (CTA→TTA)	EO946_RS16995 ←	IS3 family transposase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP034943	3,285,713	0	G	A	31.1%	788.0 / inf	534	L230L (CTA→TTA)	EO946_RS16995	IS3 family transposase
Reads supporting (aligned to +/- strand): ref base G (172/194); new base A (91/74); total (265/269)
Fisher's exact test for biased strand distribution p-value = 9.16e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.27e-01

Only 100 of 534 total aligned reads displayed.

AAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTG‑AAAGTTCCGATCCAATATATTATCGACCACGGCATATGGCTGCCCAT  >  NZ_CP034943/3285576‑3285784

     tGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAATTCTAATATACTGGAAAGGTACACTTGTTTCTGTCCATCTGGCCAATATGTGATGTCCGTTACTAATGTTTcc                                                                   >  1:4689107/1‑139 (MQ=11)

     tGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCCAATATGTGATGTCCGTTACTAATTTTTCa                                                                   >  1:439743/1‑140 (MQ=255)

     tGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAATTTTTCa                                                                   >  1:1838010/1‑140 (MQ=21)

       tCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTCCAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAag                                                                 >  2:2321684/1‑140 (MQ=255)

       tCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTAGAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAag                                                                 >  1:6778050/1‑140 (MQ=255)

       tCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAag                                                                 >  2:2223843/1‑140 (MQ=40)

       tCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAag                                                                 >  2:4437208/1‑140 (MQ=40)

       tCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAag                                                                 >  1:6268767/1‑140 (MQ=40)

       tCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAag                                                                 >  2:7267371/1‑140 (MQ=40)

       tCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAag                                                                 >  1:1960249/1‑140 (MQ=40)

       tCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAGGTAGAATTGTTTCTGTCCATAAGGCAAATATGGGATGTCCGTTACTAATTTTTCAag                                                                 >  1:1348560/1‑140 (MQ=255)

       tCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAAACGTAATAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATTTCCGGTACTAGTTTTTCAag                                                                 >  2:5608817/1‑140 (MQ=255)

           aGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAggg                                                             >  1:2987088/1‑140 (MQ=40)

               aaaGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGAt                                                         <  1:2240527/140‑1 (MQ=40)

               aaaGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGAt                                                         <  2:7054991/140‑1 (MQ=40)

               aaaGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAATTTTTCAAGAGGGCGAt                                                         <  2:1311151/140‑1 (MQ=21)

                 aGTCTTTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAACGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAATTTTTAAATAGGGCGATCa                                                       >  2:3491160/1‑140 (MQ=11)

                 aGTCTGTGTCCTGCTTATCCCCAATCGTAAATGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAATTTTTCAAGAGGGCGATCa                                                       >  2:433206/1‑140 (MQ=255)

                 aGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGTGCGATCa                                                       >  2:4885632/1‑140 (MQ=255)

                 aGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCa                                                       >  2:741963/1‑140 (MQ=40)

                 aGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCa                                                       >  1:1531268/1‑140 (MQ=40)

                 aGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCa                                                       >  1:3504824/1‑140 (MQ=40)

                 aGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCa                                                       >  2:621718/1‑140 (MQ=40)

                 aGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAATTTTTCAAGAGGGCGATCa                                                       >  2:6358336/1‑140 (MQ=21)

                 aGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAATTTTTCAAGAGGGCGATCa                                                       >  2:6071365/1‑140 (MQ=21)

                 aGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTGACTAATTTTTCAAGAGGGCGATCa                                                       >  1:1589022/1‑140 (MQ=255)

                 aGTCTGTGTCCTGCTCATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCa                                                       >  2:2746868/1‑140 (MQ=255)

                 aGTCTGTGTCCTGCGTATCCCCAATCGTAAACGCACTCACTTCTCCATTGTATACATCTAATATACTGTAACGGTAGAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCACGAGGGCGATCa                                                       >  1:7640879/1‑140 (MQ=14)

                     tgtgTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACt                                                   >  2:5070744/1‑140 (MQ=40)

                       tgtCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAATTTTTCAAGAGGGCGATCAGACTG‑a                                                <  1:7117921/140‑1 (MQ=21)

                           cTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAATTTTTCAAGAGGGCGATCAGACTG‑AAAGt                                            >  1:417302/1‑140 (MQ=21)

                             gCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTG‑AAAGTTc                                          >  2:6616011/1‑140 (MQ=40)

                             gCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTG‑AAAGTTc                                          <  1:5685189/140‑1 (MQ=40)

                             gCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAATTTTTCAAGAGGGCGATCAGACTG‑AAAGTTc                                          >  2:6876269/1‑140 (MQ=21)

                             gCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAATTTTTCAAGAGGGCGATCAGACTG‑AAAGTTc                                          <  2:2858806/140‑1 (MQ=21)

                             gCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAATTTTTCAAGAGGGCGATCAGACTG‑AAAGTTc                                          <  2:2699803/140‑1 (MQ=21)

                             gCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAATTTTTCAAGAGGGCGATCAGACTG‑AAAGTTc                                          >  1:2025047/1‑140 (MQ=21)

                             gCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATAGACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAATTTTTCAAGAGGGCGATCAGACTG‑AAAGTTc                                          <  1:3316430/140‑1 (MQ=255)

                             gCTAATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTCTAAATCTAATATACTGGAAAGGTACAATGGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAATTTTTCAAGAGGGCGATCAGACTG‑AAAGTTc                                          <  2:5775849/140‑1 (MQ=11)

                              cTTATCCCCAATCGTAAAAGCACTCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTG‑AAAGTTcc                                         <  1:5530893/140‑1 (MQ=255)

                              cTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTG‑AAAGTTcc                                         <  1:264042/140‑1 (MQ=40)

                              cTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAATTTTTCAAGAGGGCGATCAGACTG‑AAAGTTcc                                         >  1:490572/1‑140 (MQ=21)

                               ttATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATCCTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAATTTTTCAAGAGGGCGATCAGACTG‑AAAGTTCCg                                        <  2:3923281/140‑1 (MQ=255)

                                tATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTG‑AAAGTTCCGa                                       <  2:864356/140‑1 (MQ=39)

                                 aTCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAATTTTTCAAGAGGGCGATCAGACTG‑AAAGTTCCGAt                                      >  1:4825068/1‑140 (MQ=18)

                                 aTCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAATTTTTCAAGAGGGCGATCAGACTG‑AAAGTTCCGAt                                      >  1:4269777/1‑140 (MQ=18)

                                  tCCCCACTCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTG‑AAAGTTCCGATc                                     <  2:1441910/140‑1 (MQ=37)

                                  tCCCCACTCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTG‑AAAGTTCCGATc                                     <  2:4806147/140‑1 (MQ=37)

                                  tCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTG‑AAAGTTCCGATc                                     <  2:3384057/140‑1 (MQ=37)

                                  tCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTG‑AAAGTTCCGATc                                     <  1:2511023/140‑1 (MQ=37)

                                  tCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTG‑AAAGTTCCGATc                                     <  2:5339483/140‑1 (MQ=37)

                                  tCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAATTTTTCAAGAGGGCGATCAGACTG‑AAAGTTCCGATc                                     <  2:3128863/140‑1 (MQ=17)

                                  tCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAATTTTTCAAGAGGGCGATCAGACTG‑AAAGTTCCGATc                                     <  1:5056484/140‑1 (MQ=17)

                                   ccccAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTG‑AAAGTTCCGATcc                                    >  1:3225755/1‑140 (MQ=37)

                                   ccccAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTG‑AAAGTTCCGATcc                                    >  1:2808696/1‑140 (MQ=37)

                                   ccccAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTG‑AAAGTTCCGATcc                                    >  1:387605/1‑140 (MQ=37)

                                   ccccAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTG‑AAAGTTCCGATcc                                    >  1:1023460/1‑140 (MQ=37)

                                   ccccAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATAGGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTG‑AAAGTTCCGATcc                                    >  1:2461444/1‑140 (MQ=37)

                                   ccccAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAATTTTTCAAGAGGGCGATCAGACTG‑AAAGTTCCGATcc                                    >  1:1070974/1‑140 (MQ=14)

                                   ccccAATCGTAAAAGCAATCACTTCTCCATTGTATAAATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAATTTTTCAAGAGGGCGATCAGACTG‑AAAGTTCCGATcc                                    >  1:2091253/1‑140 (MQ=14)

                                    cccAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTG‑AAAGTTCCGATCCa                                   <  2:1715594/140‑1 (MQ=35)

                                    cccAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTG‑AAAGTTCCGATCCa                                   <  2:4543816/140‑1 (MQ=35)

                                      cAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTG‑AAAGTTCCGATCCAat                                 <  2:541012/140‑1 (MQ=34)

                                      cAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTG‑AAAGTTCCGATCCAat                                 <  2:592715/140‑1 (MQ=34)

                                      cAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTGTCAAGAGGGCGATCAGACTG‑AAAGTTCCGATCCAat                                 <  2:2013380/140‑1 (MQ=21)

                                        aTCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTGCGTGACTAGTTTTTCAAGAGGGCGATCAGACTG‑AAAGTTCCGATCCAatat                               >  1:839811/1‑140 (MQ=21)

                                        aTCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTG‑AAAGTTc                                          >  2:6816451/1‑129 (MQ=35)

                                        aTCCTAAAAGCAATCACTTCGCCATTGTATACATCTAATATACTGGAAAGTTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTATTTTTTCAAGAGGGCGATCAGACTG‑AAAGTTCCTATCCAatat                               <  1:3966070/140‑1 (MQ=11)

                                                   aaccACTTCTCCATTGTATACATCTAATATCCTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTG‑AAAGTTCCGATCCAATATATTATCGACCa                    <  1:4341924/137‑1 (MQ=17)

                                                   aaacACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATACGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTG‑AAAGTTCCGATCCAATATAGTATCGACCa                    <  1:3774097/137‑1 (MQ=12)

                                                   aaTCTCGTCTCCATTGTATACATCTAATATACTGGATAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTG‑AAAGTTCCGATCCAATATATTATCGACCa                    <  2:4739896/140‑1 (MQ=12)

                                                   aaTCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTG‑AAAGTTCCGATCCAATATATTATCGACCa                    <  1:2337434/140‑1 (MQ=32)

                                                   aaTCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTG‑AAAGTTCCGATCCAATATATTATCGACCa                    <  1:3136767/140‑1 (MQ=32)

                                                   aaTCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTG‑AAAGTTCCGATCCAATATATTATCGACCa                    <  1:391210/140‑1 (MQ=32)

                                                   aaTCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTG‑AAAGTTCCGATCCAATATATTATCGACCa                    <  2:6510414/140‑1 (MQ=32)

                                                   aaTCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTG‑AAAGTTCCGATCCAATATATTATCGACCa                    <  1:4086736/140‑1 (MQ=32)

                                                   aaTCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTG‑AAAGTTCCGATCCAATATATTATCGACCa                    <  2:7515448/140‑1 (MQ=32)

                                                   aaTCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTG‑AAAGTTCCGATCCAATATATTATCGACCa                    <  1:5152627/140‑1 (MQ=32)

                                                   aaTCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTG‑AAAGTTCCGATCCAATATATTATCGACCa                    <  1:1264451/140‑1 (MQ=32)

                                                   aaTCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTG‑AAAGTTCCGATCCAATATATTATCGACCa                    <  1:7506629/140‑1 (MQ=32)

                                                   aaTCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTG‑AAAGTTCCGATCCAATATATTATCGACCa                    <  1:7387521/140‑1 (MQ=32)

                                                   aaTCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTG‑AAAGTTCCGATCCAATATATTATCGACCa                    <  1:6658000/140‑1 (MQ=32)

                                                   aaTCACTTCTCCATTGTATACATCTAATAGACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATCTGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTG‑AAAGTTCCGATCCAATATATTATCGACCa                    <  2:1877842/140‑1 (MQ=17)

                                                   aaTCACTTCTCCATTGTATACATATAAGATACTGGAAAGGTACCATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTG‑AAAGTTCCGATCCAATATATTATCGACCa                    <  2:4212520/140‑1 (MQ=12)

                                                   aaTCACTTCTCCAGTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTG‑AAAGTTCCGATCCAATATATTATCGACCa                    <  2:5659877/140‑1 (MQ=17)

                                                    aTCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTGAAAAGTTCCGATCCAATATATTATCGACCa                    <  2:1077429/140‑1 (MQ=17)

                                                           ctcCATTGTATACATCTAATATACTGGAAAGGTCCAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTG‑AAAGTTCCGATCCAATATATTATCGACCACGGCatat            >  1:5246657/1‑140 (MQ=17)

                                                            accATTGTATACATCGAATATCCTGGAAAGGTACAATTGTTTCTCTCCATCAGGCAAATCTGTGAGGTCCGTTACTAGTTTTTCAAGCGGGCGATCAGACTG‑AAAGTTCCGATCCAATATATGATCGACCACGGCATATg           <  1:6350778/139‑1 (MQ=11)

                                                                  gTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTG‑AAAGTTCCGATCCAATATATTATCGACCACGGCATATGGCTGcc     <  1:6136138/140‑1 (MQ=32)

                                                                   tataCATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATCAGGCCAATATGTGATGTCCGGTACTAGTTTTTCAAGAGGGCGATCAGACTG‑AAAGTTCCGATCCAATATCTTATCGACCACGGCATATGGCTGccc    >  1:6549744/1‑140 (MQ=255)

                                                                   tataCATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTG‑AAAGTTCCGATCCAATATATTATCGACCACGGCATATGGCTGccc    >  1:7001389/1‑140 (MQ=32)

                                                                   tataCATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTG‑AAAGTTCCGATCCAATATATTATCGACCACGGCATATGGCTGccc    >  1:5804731/1‑140 (MQ=32)

                                                                   tataCATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTG‑AAAGTTCCGATCCAATATATTATCGACCACGGCATATGGCTGccc    >  2:3033847/1‑140 (MQ=32)

                                                                   tataCATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTG‑AAAGTTCCGATCCAATATATTATCGACCACGGCATATGGCTGccc    >  2:118671/1‑140 (MQ=32)

                                                                     taCATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTCGTTTTTCAAGAGGGCGATCAGACTG‑AAAGTTCCGATCCAATATATTATCGACCACGGCATAGGGCTGCCCAt  >  2:3234678/1‑140 (MQ=17)

                                                                     taCATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTG‑AAAGTTCCGATc                                     <  2:7690406/105‑1 (MQ=32)

                                                                     taCATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTG‑AAAGTTCCGATCCAATATATTATCGACCACGGCATATGGCTGCCCAt  >  1:7224010/1‑140 (MQ=32)

                                                                     taCATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTG‑AAAGTTCCGATCCAATATATTATCGACCACGGCATATGGCTGCCCAt  >  1:5899617/1‑140 (MQ=32)

                                                                     taCATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTG‑AAAGTTCCGATCCAATATATTATCGACCACGGCATATGGCTGCCCAt  >  1:6428916/1‑140 (MQ=32)

                                                                     taCATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTG‑AAAGTTCCGATCCAATATATAATCGACCACGGCATATGGCTGCCCAt  >  2:4956993/1‑140 (MQ=17)

AAGTATGTCTAAGACAAAGTCTGTGTCCTGCTTATCCCCAATCGTAAAAGCAATCACTTCTCCATTGTATACATCTAATATACTGGAAAGGTACAATTGTTTCTGTCCATAAGGCAAATATGTGATGTCCGTTACTAGTTTTTCAAGAGGGCGATCAGACTG‑AAAGTTCCGATCCAATATATTATCGACCACGGCATATGGCTGCCCAT  >  NZ_CP034943/3285576‑3285784

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`